US EAS 801 UGANDA STANDARD. First Edition Soya protein products Specification. Reference number US EAS 801: 2014

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UGANDA STANDARD US EAS 801 First Edition 2014-10-15 Soya protein products Specification Reference number US EAS 801: 2014 UNBS 2014

US EAS 801: 2014 Compliance with this standard does not, of itself confer immunity from legal obligations A Uganda Standard does not purport to include all necessary provisions of a contract. Users are responsible for its correct application UNBS 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written permission from UNBS. Requests for permission to reproduce this document should be addressed to The Executive Director Uganda National Bureau of Standards P.O. Box 6329 Kampala Uganda Tel: 256 41 505 995 Fax: 256 41 286 123 E-mail: unbs@infocom.co.ug Web: www.unbs.go.ug ii UNBS 2014 - All rights reserved

US EAS 801: 2014 National foreword Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Tourism, Trade and Industry established under Cap 327, of the Laws of Uganda. UNBS is mandated to co-ordinate the elaboration of standards and is (a) a member of International Organisation for Standardisation (ISO) and (b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and (c) the National Enquiry Point on TBT/SPS Agreements of the World Trade Organisation (WTO). The work of preparing Uganda Standards is carried out through Technical Committees. A Technical Committee is established to deliberate on standards in a given field or area and consists of representatives of consumers, traders, academicians, manufacturers, government and other stakeholders. Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the general public for comments. The committee reviews the comments before recommending the draft standards for approval and declaration as Uganda Standards by the National Standards Council. This Uganda Standard, US EAS 801: 2014, Soya protein products Specification, is identical with and has been reproduced from an East African Standard, EAS 801: 2014, Soya protein products Specification, and adopted as a Uganda Standard. Wherever the words, East African Standard" appear, they should be replaced by "Uganda Standard." UNBS 2014 - All rights reserved iii

EAS 801: 2014 ICS 67.060 EAST AFRICAN STANDARD Soya protein products Specification EAST AFRICAN COMMUNITY EAC 2014 First Edition 2014

EAS 801: 2014 Copyright notice This EAC document is copyright-protected by EAC. While the reproduction of this document by participants in the EAC standards development process is permitted without prior permission from EAC, neither this document nor any extract from it may be reproduced, stored or transmitted in any form for any other purpose without prior written permission from EAC. Requests for permission to reproduce this document for the purpose of selling it should be addressed as shown below or to EAC s member body in the country of the requester: East African Community 2014 All rights reserved East African Community P.O.Box 1096 Arusha Tanzania Tel: 255 27 2504253/8 Fax: 255 27 2504481/2504255 E-mail: eac@eachq.org Web: www.eac-quality.net Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. Violators may be persecuted ii EAC 2014 All rights reserved

EAS 801: 2014 Foreword Development of the East African Standards has been necessitated by the need for harmonizing requirements governing quality of products and services in the East African Community. It is envisaged that through harmonized standardization, trade barriers that are encountered when goods and services are exchanged within the Community will be removed. In order to achieve this objective, the Community established an East African Standards Committee mandated to develop and issue East African Standards. The Committee is composed of representatives of the National Standards Bodies in Partner States, together with the representatives from the private sectors and consumer organizations. Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the Partner States. The comments received are discussed and incorporated before finalization of standards, in accordance with the procedures of the Community. East African Standards are subject to review, to keep pace with technological advances. Users of the East African Standards are therefore expected to ensure that they always have the latest versions of the standards they are implementing. EAS 801 was prepared by Technical Committee EAS/TC 014, Cereal, pulses and derived products In preparation of this standard assistance was derived from: Codex Standard 175, General Standard for Soy Protein Products. EAC 2014 All rights reserved iii

EAS 801: 2014 Introduction Soya products are becoming a mainstream part of regional dietary items. The benefit associated with the products includes among others the richness in protein supply. This is of great benefit especially as an alternative protein source. However, it is important to note that soya has naturally high level of anti-nutritional properties related to trypsin inhibitor and tannins. The manufacturers are advised to apply appropriate processing technologies to reduce these anti-nutrient properties to acceptable safe levels for human consumption. Soya Protein Products (SPP) are foods produced by removing or decreasing certain major non-protein constituents (water, oil, carbohydrates) from soya beans or edible full fat soya flour in a manner that achieves high protein commercial products with different protein content such as Soya protein flour / Defatted Soya Protein flour (SPF or DSF), Soya Protein Concentrates (SPC), and Soya Protein Isolates (SPI). This standard has been developed to ensure the safety and quality of edible full fat soya flour for human consumption. iv EAC 2014 All rights reserved

EAST AFRICAN STANDARD EAS 801: 2014 Soya protein products Specification 1 Scope This East African Standard specifies requirements and methods of sampling and test for soya protein products intended for human consumption. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. AOAC 972.25, Lead in Food, atomic absorption spectrophotometric method EAS 38, Labelling of pre-packaged foods Specification EAS 39, Hygiene in the food and drink manufacturing industry Code of practice EAS 762, Dry soybeans Specification EAS 799, Edible full fat soya flour Specification ISO 2171, Cereals, pulses and by-products Determination of ash yield by incineration ISO 5498, Agricultural food products Determination of crude fibre content General method ISO 6579, Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp ISO 7251, Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of presumptive Escherichia coli Most probable number technique ISO 11085, Cereals, cereals-based products and animal feeding stuffs Determination of crude fat and total fat content by the Randall extraction method ISO 16050, Foodstuffs Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products High-performance liquid chromatographic method ISO 20483, Cereals and pulses Determination of the nitrogen content and calculation of the crude protein content Kjeldahl method ISO 21527-2, Microbiology of food and animal feedstuffs Horizontal method for the enumeration of yeasts and moulds Part 2: Colony count technique in products with water activity less than or equal to 0.95 ISO 24333, Cereals, and cereal products Sampling ISO 24557, Pulses Determination of moisture content Air oven EAC 2014 All rights reserved 1

EAS 801: 2014 3 Terms and definitions For the purposes of this standard, the following terms and definitions shall apply 3.1 soya bean whole mature dry bean of Glycine max (L) Merr. varieties 3.2 Soya Protein Product (SPP) foods produced by removing or decreasing certain major non-protein constituents (water, oil, carbohydrates) from soya beans or edible full fat soya flour in a manner that achieves high protein products with different protein content 4 Classification of soya protein products Soya protein products shall be classified based on different protein content as follows: a) Defatted Soya protein Flour or Soya Protein Flour (DSF or SPF); b) Soya Protein Concentrate (SPC); and c) Soya Protein Isolate (SPI), 5 Requirements 5.1 Raw material Raw material shall include: a) dry soya bean complying with EAS 762; or b) edible full fat soya flour complying with EAS 799, 5.2 Optional ingredients In the preparation of soya protein products, the following ingredients, complying with relevant East African and CODEX standards, may be used: a) salt; b) carbohydrates, including sugars; c) edible oils; d) minerals and vitamins; e) herbs and spices; or f) other protein products. 5.3 General requirements Soya protein products shall: 2 EAC 2014 All rights reserved

EAS 801: 2014 a) have taste and odour characteristic of product and any approved food additives used; b) be free from rancidity and mustiness; c) be free from extraneous and foreign matter; d) be free from pathogenic micro-organisms and substances originating from micro-organisms; and e) free from any other deleterious substances in amounts which may constitute a health hazard. 5.4 Specific quality requirements Soya protein products shall comply with the quality requirements specified in Table 1. Table 1 Specific quality requirements for soya protein products S/N Characteristic Requirement Soya Protein Flour Test method Soya Protein Concentrate Soya Protein Isolate (SPC) (SPI) 10.0 10.0 10.0 ISO 24557 50-65 65-90 >90 ISO 20483 (SPF or DSF) i. Moisture, % m/m, max. ii. Protein (N x 6.25)*, % m/m iii. Soya oil (fats on dry basis), % m/m, max. 1.0 1.0 1.0 ISO 11085 iv. Total ash, % m/m, max. 8.0 8.0 8.0 ISO 2171 v. Crude fibre, % m/m, max. 5.0 6.0 0.5 ISO 5498 * Soya protein products shall meet the requirements basing on Protein content on a dry weight basis excluding added vitamins, minerals, amino acids and food additives 6 Hygiene Soya protein products shall be prepared and handled in a hygienic manner in accordance with EAS 39 and shall conform to the microbiological limits stipulated in Table 2. Table 2 Microbiological limits for soya protein products S/N Micro-organism Maximum limit Test method I. Escherichia coli, MPN/g Not detected ISO 7251 II. Salmonella spp per 25 g Absent ISO 6579 III. Yeast and moulds, cfu/g EAC 2014 All rights reserved 10 3 ISO 21527-2 3

EAS 801: 2014 7 Contaminants 7.1 Pesticide residues Soya protein products shall comply with those maximum pesticide residue limits established by Codex Alimentarius Commission for similar commodities. NOTE Where the use of certain pesticide is prohibited by some Partner States, it shall be notified to all other Partner States accordingly. 7.2 Other contaminants When tested in accordance with AOAC 972.25, the level of Lead (Pb) in soya protein products shall not exceed 0.2 mg/kg. The product shall not contain other heavy metal contaminants in amounts which may represent a hazard to health. 7.3 Aflatoxins The maximum content of aflatoxins in soya protein products shall not exceed 5 µg/kg (ppb) for aflatoxin B 1 and 10 µg/kg for total aflatoxins when tested in accordance with ISO 16050. 8 Packaging 8.1 Soya protein products shall be packaged in, suitable, clean, sound, free from insect, fungal infestation food grade material. 8.2 Soya protein products shall be packed in containers which will safeguard the hygienic, nutritional, technological and organoleptic qualities of the products. 8.3 Each package shall be securely sealed. 8.4 The quantity and fill of Soya protein products shall comply with the Weights and measures regulations of Partner States or equivalent legislation. 9 Labelling In addition to the requirements given in EAS 38, containers packed with soya protein products shall be legibly and indelibly labelled with the following information; a) name of the product shall be Soya protein flour/defatted soya flour or Soya protein concentrate or Soya protein isolate ; b) brand name/trade name; c) name and physical address of the manufacturer; d) lot identification/ batch/code number; e) net weight in metric units; f) list of ingredients in descending order; g) list of any food additives used; h) date of manufacture; 4 EAC 2014 All rights reserved

EAS 801: 2014 i) country of origin; j) storage conditions; k) expiry date; and l) language shall be English. Any additional official language may be used. 10 Method of sampling Sampling shall be done in accordance with ISO 24333.. EAC 2014 All rights reserved 5

EAS 801: 2014 EAC 2014 All rights reserved