Key words: Rapid diagnosis technique, Co-agglutination, Latex agglutination Counter immunoelectrophoresis, Cupsul antigens

Transcription

1 Key words: Rapid diagnosis technique, Co-agglutination, Latex agglutination Counter immunoelectrophoresis, Cupsul antigens,

2 Table 1 Sensitivity of CoA, and CIE

3 Table 2 Results of detection of infectious agents in clinical specimens by CoA and LA as compared with conventional method Table 3 Comparison of serological and conventional techniques for detection of H. influenzae from a patient with meningitidis purulenta note: NT is not tested

4 Table 4 Comparison of serological and conventional techniques for detection of S.pneumoniae from a patient with meningitidis purulenta note: NT is not tested Table 5 Comparison of CoA and LA for rapid detection of specific antigens in clinical specimens

5 Table 6 Infectious agents detected in sputum with a pneumonia

6 1) Chistensen, P., Kalmeter, G., Jonsson, S. & Kronvall, G.: A new method for the serological grouping of streptococci with specific antibodies to protein A-containing sta- phylococci. Infect. Immunity, 7: , ) Edwards, E. A. & Larson, G.: Serological grouping of hemolytic streptococci by counterimmunoelectrophoresis. Appl. Microbiol., 26: , ) Kronvall, G.: A rapid slide-agglutination method for typing pneumococci by means of specific antibody absorbed to protein A- containing staphylococci. J. Med. Microbiol., 6: , ) Danielsson, D. & Kronvall, G.: Slide agg-

7 lutination method for serological identification of Neisseria gonorrhoeae with anti-gonorrheal antibody absorbed to protein A-containing staphylococci. Appl. Microbiol., 27: , )Olcen, P.: Serological methods for rapid diagnosis of Haemophilus influenzae, Neisseria meningitidis an Streptococcus pneumoniae in cerebrospinal fluid: A comparison of co-agglutination, immunofluorescence and immunoelectroosmophoresis. Scand. J. Infect. Dis., 10: , ) Thirumoorthi, M. C. & Dajani, A. S.: Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunolectrophoresis for bacterial antigen detection. J. Clin. Microbiol., 9: 28-32, ) Edwards, E. A., Kilpartrick, M. E. & Hooper, D.: Rapid detection of pneumococcal antigens in sputum and blood serum using a coagglutination test. Milit. Med., 145 : , ) Beneddict, L., Wasilauskas & Kenneth, D. Hampton: Determination of bacterial meningitis: A retrospective study of 80 cerebrospinal fluid specimens evaluated by four in vitro methods. J. Clin. Microbiol., 16: , ) Fones, D.M.: The rapid detection of bacterial anterial antigens. Recnt Advances in Infection. Pub. Churchill: , ) Dirks-Go, S. I. S. & Zanen,H. C.: Latex agglutination, counterimmunoelectrophoresis and protein A co-agglutination in diagnosis of bacterial meningitis. J. Clin. Path., 31: , ) Lindberg, A. A.: Methods for the laboratory investigation of bacterial respiratory infections. Meeting on rapid diagnosis in acute respiratory infections. World Health Organization, BAC/ARI/ ) Kalin, M., Lindberg, A. A. & Olausson, E. H.: Diagnosis of pneumococcal by coagglutination and counterimmunoelectrophoresis of sputum samples. Eur. J. Clin. Microbiol., 1: 91-96, ) Suksanong, M. & Danjiani, A. S.: Detection of haemophilus infleunzae type b antigens in body fluids, using specific antibody-coated staphylococci. J. Clin. Microbiol., 5: 81-85, ) David, L., Ingram., et al.: Detection of the capsular polysaccharide of haemophilus influenzae, type b, in body fluids. In. S. H. Sell & Kerzon, D. T. Haemophilus influenzae Vanderbilt University Press, Nashrille, 37-43, 1973.

8 Serological Techniques for Detection of Capsul Antigens on Clinical Specimens Masanori AIHARA, Yoshikazu HASHIMOTO, Tamaki NAKAMURA, Masako MATSUMOTO, Akihiko UEHARA,Masanari JHOKI,Midori ARAI,Michiko SAKAI, Hisae NADA & Hiroshi TAKAHASHI Department of Clinical Pathology,Tenri Hospital As a rapid method of diagnosis of bacterial infections, co-agglutination (CoA), latex agglutination (LA) and counter immunoelectrophoresis (CIE) were tested in order to detect the capsul antigens of pneumococcus and H.influenzae in specimens from patients. A comparative study with these three serological tests showed that CoA test was four to thirty-two times sensitive as LA and CIE. From 136 specimens of cerebrospinal fluid (CSF),two cases of meningitidis purulenta demonstrated H.influenzae and pneumococcus respectively by both serological and conventional methods.in the case of pneumococcal meningitidis,the antigen was detected by CoA and LA during two days after chemotherapy commenced, although negative results were obtained by the conventional method. From 97 sputum specimens,capsul antigens were detected by CoA and LA in 16 specimens; especially CoA tests were positive for H.influenzae type b in two cases, for H. influenzae type a, cf in seven cases and for pneumococcal antigens in 11 cases,whereas nine antigen were detected by LA test. Positive CIE test was seen in only two clinical specimens;csf from H. influenzae type b meningitis and sputum from pneumococcal pneumoniae. CoA test was found to be a useful tool for a rapid diagnosis of these bacterial infections as compared with other serological tests such as LA and CIE for detection of pneumococcus and H.influenzae antigens on clinical specimens.