Diversity and Tropism of HIV-1 Plasma Rebound Virus after Treatment Discontinuation

Transcription

1 Diversity and Tropism of HIV-1 Plasma Rebound Virus after Treatment Discontinuation By: Blake M. Hauser Senior Honors Thesis Department of Biology College of Arts and Sciences The University of North Carolina at Chapel Hill April 20, 2016 Approved: Dr. Ronald Swanstrom, Thesis Advisor Distinguished Professor of Biochemistry and Biophysics

2 ABSTRACT Modern antiretroviral therapies can confer effective suppression of HIV-1 infection. However, HIV-infected people discontinuing antiretroviral therapy experience a rebound of virus from a persistent reservoir. Characterizing this reservoir constitutes a crucial step towards developing a cure, and we hypothesized that assessing the diversity and tropism of rebound virus would provide insight into the types of cells that likely house the viral reservoir as well as reservoir diversity. We examined 10 rebound samples from a project within the large-scale AIDS Clinical Trials Group and used single genome amplification and Primer ID deep sequencing to assess the genetic diversity of the env gene, which encodes the HIV-1 envelope protein, among rebounding virus. Samples from the same patients with viral suppression due to antiretroviral therapy were also available. Most rebound virus populations showed significant diversity. All env genes examined were isoforms that would require cells to express high surface levels of the CD4 receptor for entry, consistent with the current hypothesis that virus is selectively replicated in CD4+ T cells. These results indicate that most people discontinuing therapy release a diverse population of virus, and this released virus targets CD4+ T cells rather than myeloid cells, which tend to last longer. Research indicates that a small proportion of viruses have evolved to efficiently enter myeloid cells. Current HIV-1 cure strategies focus on reactivating latent HIV-1 and targeting the resultant virus. It is essential to understand the features of rebound viruses because they are the first such targets. 2

3 INTRODUCTION HIV-1 causes approximately 6,300 new infections daily. Successful antiretroviral therapy (ART) reduces HIV-1 viral load in the blood down to stable low levels; however ART does not eliminate virus. Developing a cure for HIV-1 constitutes one of the top priorities in the HIV-1 research field today, and such a treatment has the potential to end the dependency on ART that thousands currently have. Since ART does not fully eliminate HIV-1 infection, most people discontinuing therapy experience a rebound of virus from a persistent reservoir [1-4]. The variable timing of rebound, where the virus is coming from, and the complexity of the rebound virus are poorly understood. One potential source of virus is latently infected resting CD4+ T cells. These immune cells are well-established targets of HIV-1 infection. There is also a continual production of virus at low levels in spite of ART, and other cell types could be the source of rebound virus. This includes myeloid cells, which are differentiated from the bone marrow and can perform immune functions. In a largely quiescent reservoir, the rebound virus could be clonal as the result of virus from a single cell leaving a latent state. However, the number/complexity of the rebound virus within a person has recently been predicted to be much higher than predicted based on a largely quiescent reservoir [4]. To this end we have examined peripheral blood samples from ACTG study A5068 [5] to investigate the diversity and entry phenotype of rebound virus. Subjects in A5068 discontinued ART as part of an analytic treatment interruption (ATI) and had virologic rebound detectable in the blood on average between 2-4 weeks after discontinuation of therapy. To study the complexity of the rebound virus we assessed the genetic diversity of the viral env gene and also examined viral entry phenotype. Phylogenetic analysis showed a high degree of diversity in the 3

4 rebound virus population. In addition, all Env proteins examined required high levels of CD4 for efficient entry, consistent with prior selection for replication in CD4+ T cells. Our analysis suggests that a viral population replicating in myeloid cells is not a significant source of rebound virus found early in the blood. 4

5 METHODS Study Population The AIDS Clinical Trails Group (ACTG) study A5068 examined whether structured treatment interruptions and/or vaccination with an exogenous HIV vaccine that consisted of an ALVAC-HIV vector was associated with control of viral replication during a subsequent analytical treatment interruption. This study included a control group with no vaccination or structured interruption control for comparison (Arm A). The study showed a modest effect in reduced peak and set point viral load of the rebound virus in the arms that included the structured treatment interruptions [5]. Subjects with the analytical treatment interruption only (Arm A) had a median CD4+ T cell count of 779 copies/ml, and viral RNA levels of less than 50 copies/ml at entry into the study while still on therapy. For our study, we used blood plasma samples from 10 of these subjects early after the initiation of the analytical treatment interruption when viral load was at or above 1,000 copies/ml (Figure 6). A pre-therapy sample was also available for one subject. Single Genome Amplification Sequences of virion RNA were examined using single genome amplification (SGA) [6] of the full-length env gene. SGA aims to isolate cdna specific to a single virion, allowing researchers to obtain individual sequences corresponding to single virions within a patient sample. Prior to HIV-1 RNA isolation from blood plasma using the QIAmp Viral RNA Mini Kit (Qiagen), samples were pelleted by centrifugation at 25,000 xg for 2 hours at 4 ºC. Isolated vrna was reverse transcribed using Superscript III Reverse transcriptase (Invitrogen) and an oligo-d(t) primer. SGA was accomplished by endpoint dilutions and semi-nested PCR using 5

6 Platinum Taq High Fidelity polymerase (Invitrogen) and primers B3F3 (5 - TGGAAAGGTGAAGGG GCAGTAGTAATAC-3 ) LTR DN (5 - GACTCTCGAGAAGCACTCAAGGCAAGCTTTATTGAG-3 ) and B5957 UP1 (5 - GATCAAGCTTTAGGCATCTCCTATGGCAGGAAGAAG-3 ) and LTR DN for round one and two of PCR, respectively. Sequencing of individual PCR amplified env amplicons was performed, and it was confirmed that no double peaks were present in the sequencing output prior to phylogenetic analysis (as this would have indicated the simultaneous detection of more than one viral sequence). Next Generation Sequencing using Primer ID Sequences of virion RNA were further examined using deep sequencing with Primer ID [7] of the V1-V3 region of the env gene. Primer ID uses unique barcodes to tag each DNA template during the PCR process [7]. This allows investigators to validate the number of templates initially present in addition to lowering the sequencing error rate to 1 in 10,000 bases [7]. We used the previously described protocol to construct Primer ID sequencing libraries from viral RNA. [7] In brief, the cdna primer with a block of 9 degenerate nucleotides (Primer ID) was used to tag each viral template with a unique Primer ID. After purification of cdna using magnetic beads, we used two rounds of PCR to amplify Primer ID tagged cdna and to add Illumina barcoded adaptor sequences for multiplexing. We used MiSeq 300 bp pair-end platform to sequence the constructed library. Raw sequencing reads were initially de-multiplexed by the Illumina pipeline. We used the previously described in-house pipeline to retrieve template consensus sequences [7]. 6

7 Determination of Major Lineages The minimum number of rebound viruses was estimated by analyzing distinct sequence lineages using both the phylogenetic tree analysis and highlighter plots (Figures 1D, 2A, 3, 4) of each subject. Both distinct lineages and complex clusters of linked sequence differences were used. This means that we initially counted major clusters on the phylogenetic trees, and we then used the highlighter plot to analyze the diversity of different regions (5 in total of about 600 basepairs per region) of the env gene and to counting clusters of similarly mutated regions. The lowest number of lineages was chosen. This was all done to avoid interpreting complexity that could have been the result of post-rebound recombination. These values are minimum estimates, as we took the lowest number of lineages counted per subject of the rebound virus complexity. Virus Characterization Phylogenetic analysis of the full-length env genes was used to select a representative sampling of amplicons for cloning to assess viral entry phenotype. env genes cloned in an expression vector were used in cotransfection with the pnl4-3.lucr-e reporter backbone plasmid into 293T cells to generate an Env-pseudotyped luciferase reporter virus as described previously [8-10]. By comparing the ability of the pseudotyped virus to infect Affinofile cells expressing high versus low levels of surface CD4, we are able to consistently assess viral CD4 entry phenotype. This allows an assignment of viral entry tropism as being largely restricted to CD4+ T cells when requiring high levels of CD4 for entry (T cell-tropic) versus being able to efficiently infect cells with a low density of CD4, as is found on macrophages (macrophagetropic) [8]. Sensitivity to soluble CD4 was used to confirm the CD4 entry phenotype. Additionally, coreceptor usage was analyzed using Maraviroc inhibition of CCR5. 7

8 RESULTS We analyzed eleven plasma samples from ten subjects (subject had a pre-therapy ( ) and rebound (R1) sample available; Figure 1A). We used plasma from the first available time point with a viral load above 1,000 copies/ml to focus on the early rebound virus. Most samples had viral loads less than 10,000 copies/ml (Figure 1B). Eight of ten subjects had nadir CD4+ T cell counts prior to therapy recorded as between cells/ml, while one (sample R10) was <201 cells/ml. In order to determine the viral sequence diversity of the rebound samples, we employed deep sequencing using Primer ID to sequence the V1-V3 region of the env gene (Figure 1D), in addition to doing SGA of the full length env gene. Deep sequencing results were achieved for eight of ten rebound samples and the pre-therapy sample (however, the matching rebound sample R1 was unsuccessful). We estimated the minimum number of rebound viruses as distinct sequence lineages in the phylogenetic tree analysis and highlighter plots (Figures 1D, 2A, 3, 4). We used both distinct lineages and complex clusters of linked sequence differences in forming this estimate while trying to avoid interpreting complexity that could have been the result of post-rebound recombination. We consider these values as minimum estimates of the rebound virus complexity since the viruses considered as potential recombinants could have pre-existed in the reservoir and been independently reactivated. Analysis of the minimum number of lineages versus time (in weeks) to rebound demonstrated no obvious correlation between time to rebound and complexity of the rebound virus population (Figure 1C). For virus that rebounded in the first few weeks we estimated between 1 and 5 distinct lineages by deep sequencing, with a mean of 3.1 lineages, for these early rebound viruses. R2 took the longest to rebound (10 weeks) and showed relatively low diversity with only 2 lineages. SGA results of full length env genes 8

9 showed between 1 and 4 distinct lineages, with a mean of 3.1 lineages, which is similar to the results obtained with deep sequencing (Figure 1C). The mean intrapatient diversity was also determined by pairwise analysis using full-length env and ranged from 0.2% to 3.1%. Additional analyses of subjects early in infection (10 subjects) [11] and chronically infected subjects (7 subjects, unpublished observation) were also done and ranged between 0.16% to 1.2% for early infection subjects and 1.1% to 4.1% for chronically infected subjects not on therapy. These numbers suggest that the rebound virus is more similar to virus in chronically infected subjects where multiple viral lineages form a genetically complex population. We subsequently cloned individual env genes (51 total; average 4 amplicons per subject) from the major lineages in each participant to determine the entry phenotype of the rebound virus using the Affinofile cell line (Figures 2A, 4). The pre-therapy sample ( ) was also included; no clones were obtained for sample R5. Affinofile cells allow controlled expression of CD4 and are currently the most consistent method to assess CD4-dependent entry phenotype [8]. Entry phenotype was evaluated based on the ability of viruses pseudotyped with the Env proteins from the rebound virus to infect Affinofile cells expressing either high or low levels of surface CD4. The phenotype was confirmed by sensitivity to inhibition by soluble CD4 at a concentration of 5.0 µg/ml when infecting TZM-bl cells [12], a level of soluble CD4 that neutralizes macrophage-tropic viruses but not R5 T cell-tropic viruses. None of the Env proteins tested showed any ability to infect cells expressing low levels of CD4 compared to their infectivity on cells expressing high levels of CD4 as all exhibited less than 2% relative infectivity (Figure 2B). All Env proteins tested were also resistant to inhibition by soluble CD4 (Figure 2D). In addition, the CCR5 antagonist Maraviroc inhibited all pseudotyped viruses. Therefore, all isolated Env 9

10 proteins used CCR5 as the coreceptor. Taken together, all Env proteins tested from the rebound virus of all subjects had a phenotype consistent with R5 T cell-tropic virus. 10

11 DISCUSSION In this report we describe entry phenotype and population complexity of the initial virus that rebounds from the latent or low-level viremia reservoir after therapy discontinuation. These features are relevant to developing strategies of HIV-1 eradication because they are features of the first virus that must be targeted. We evaluated entry phenotype in order to determine what cell type this virus had been predominantly replicating in at the time therapy was initiated and/or when the virus entered the latent reservoir. In addition, we examined the complexity of the rebound population as a measure of the minimum number of viruses that rebound from the reservoir and can be detected in the blood. The genetic diversity of the viral reservoir and rebound virus has been debated, but it is perceived to have low diversity and to be primarily clonal or multiclonal [13, 14]. Our results are consistent with the observation of Rothenberger et al.[4] who proposed a multifocal model of virus reactivation. We observed a mean of 3.1 distinct lineages when counting major lineages with both deep sequencing and SGA. This points to a minimum of 3 viruses rebounding within a short period after treatment interruption. While this is significantly less than the 5-25 rebound/founder viruses suggested by Rothenberger et al. [4], when we reanalyzed the sequence data from that study for the 3 subjects with SGA of virion RNA using the same criteria as used in this report, we found the mean number of distinct lineages to be 3.3. This is similar to what we observed. However, since we did not count potential recombinants in our analysis, this is a minimum estimate. The number of rebound viruses could be affected by factors that affect viral reservoir size, such as duration of ART and time after infection of ART initiation. These data are not available for the subjects included in this report. Reservoir size, as measured by cell-associated viral RNA and DNA, has been shown to be correlated with set point after therapy 11

12 discontinuation [15]. Sample R2 did show one of the lowest sequence diversities (0.59%) and took the longest to have a measurable rebound virus population, but we only analyzed one subject who took longer than four weeks for rebound to be detected. The use of viral outgrowth assays from subjects on therapy after isolation of resting CD4+ T cells and reactivation in culture has established this cell type as a reservoir for HIV-1 latency [1-3]. We sought to evaluate the rebound virus population by using entry phenotype. Distinct lineages of HIV-1 can evolve the ability to use low levels of CD4 for entry, but these have been found primarily in the cerebral spinal fluid [8,11]; presumably, this localized evolution is due to selective pressure to enhance replication in cells such as macrophages, which express low levels of CD4. Here, we assessed the CD4 entry phenotype in conjunction with soluble CD4 sensitivity (a feature of low CD4-using Envs) to determine that the rebound viruses needed high levels of CD4 for efficient entry (Figure 2B). Sensitivity to inhibition by Maraviroc of all Env proteins showed that all of the viruses were CCR5-using (Figure 2C), making these viruses R5 T cell-tropic. Based on this phenotypic characterization, we can conclude that the rebound virus was adapted to replicating in CD4+ T cells at the time the reservoir was formed. These results do not define the cell type that harbored the latent virus prior to rebound (T cells or macrophages); however, these results exclude the possibility that the rebound virus was replicating extensively in a myeloid cell lineage prior to latency since this would have selected for the ability to use low levels of CD4 for entry. It should be noted that viral rebound in isolated compartments of the body, such as the brain, or viruses released later from smaller reservoirs, could have phenotypes that are distinct from the initial rebound virus that predominates in the blood. 12

13 In summary, we have shown that the early rebounding virus is more genetically diverse than previously thought. Additionally, we were able to show that all virus isolates from the plasma used the CCR5 co-receptor in the cell entry process and required high levels of CD4 for entry. Therefore, these virus isolates are R5 T cell-tropic. This excludes a population of virus replicating in myeloid cells as a significant contributor to the viral reservoir that repopulates blood early after therapy discontinuation. Elucidating the nature of this viral reservoir constitutes a crucial prerequisite to eliminating the latent reservoir, which will be required in order to cure HIV. By providing considerable insight into the genetic diversity of rebounding virus populations in addition to characterizing the tropism phenotypes of this virus, our findings also lay the groundwork for future research aimed at targeting the rebound virus population for eradication. 13

14 References 1. Finzi D, Hermankova M, Pierson T, et al. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 1997; 278: Wong JK, Hezareh M, Gunthard HF, et al. Recovery of replication-competent HIV despite prolonged suppression of plasma viremia. Science 1997; 278: Finzi D, Blankson J, Siliciano JD, et al. Latent infection of CD4+ T cells provides a mechanism for lifelong persistence of HIV-1, even in patients on effective combination therapy. Nat Med 1999; 5: Rothenberger MK, Keele BF, Wietgrefe SW, et al. Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption. Proc Natl Acad Sci U S A 2015; 112:E Jacobson JM, Pat Bucy R, Spritzler J, et al. Evidence That Intermittent Structured Treatment Interruption, but Not Immunization with ALVAC-HIV vcp1452, Promotes Host Control of HIV Replication: The Results of AIDS Clinical Trials Group Journal of Infectious Diseases 2006; 194: Salazar-Gonzalez JF, Bailes E, Pham KT, et al. Deciphering human immunodeficiency virus type 1 transmission and early envelope diversification by single-genome amplification and sequencing. Journal of Virology 2008; 82: Zhou S, Jones C, Mieczkowski P, Swanstrom R. Primer ID Validates Template Sampling Depth and Greatly Reduces the Error Rate of Next-Generation Sequencing of HIV-1 Genomic RNA Populations. Journal of Virology 2015; 89: Joseph SB, Arrildt KT, Swanstrom AE, et al. Quantification of entry phenotypes of macrophage-tropic HIV-1 across a wide range of CD4 densities. J Virol 2014; 88:

15 9. Ping LH, Cohen MS, Hoffman I, et al. Effects of genital tract inflammation on human immunodeficiency virus type 1 V3 populations in blood and semen. J Virol 2000; 74: Keele BF, Giorgi EE, Salazar-Gonzalez JF, et al. Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proc Natl Acad Sci U S A 2008; 105: Sturdevant CB, Joseph SB, Schnell G, Price RW, Swanstrom R, Spudich S. Compartmentalized replication of R5 T cell-tropic HIV-1 in the central nervous system early in the course of infection. PLoS Pathog 2015; 11:e Montefiori DC. Evaluating neutralizing antibodies against HIV, SIV, and SHIV in luciferase reporter gene assays. Curr Protoc Immunol 2005; Chapter 12:Unit Kearney MF, Spindler J, Shao W, et al. Lack of detectable HIV-1 molecular evolution during suppressive antiretroviral therapy. PLoS Pathog 2014; 10:e Joos B, Fischer M, Kuster H, et al. HIV rebounds from latently infected cells, rather than from continuing low-level replication. Proceedings of the National Academy of Sciences 2008; 105: Li JZ, Heisey A, Ahmed H, et al. Relationship of HIV reservoir characteristics with immune status and viral rebound kinetics in an HIV therapeutic vaccine study. AIDS 2014; 28:

16 0.02 ATCTAAAG_286_WM12A15_r2_combined AGGATGTG_193_WM12A15_r2_combined TCCCAGTC_104_WM12A15_r2_combined TGCCGTAC_453_WM12A15_r2_combined TATCTTGT_743_WM12A15_r2_combined TAGTCTGG_633_WM12A15_r2_combined GAGGTAAG_498_WM12A15_r2_combined CCCACCCG_3012_WM12A15_r2_combined CGGTCTTA_167_WM12A15_r2_combined GAATTGAC_3315_WM12A15_r2_combined GTATTGGA_3322_WM12A15_r2_combined CACCCGGA_2525_WM12A15_r2_combined ATTAACAG_2419_WM12A15_r2_combined CCCAGGTC_2234_WM12A15_r2_combined GAGTGGTC_2598_WM12A15_r2_combined GTGTGTTG_1444_WM12A15_r2_combined ACATTTTC_71_WM12A15_r2_combined GAGACGAG_66_WM12A15_r2_combined TGTTCCAG_1917_WM12A15_r2_combined CTGTGAAG_2789_WM12A15_r2_combined GAGGCGGT_413_WM12A15_r2_combined GAAGAAGT_57_WM12A15_r2_combined ATCGTTAC_3161_WM12A15_r2_combined GTCGACCA_1822_WM12A15_r2_combined AACCTCAG_2011_WM12A15_r2_combined CGTTTACC_830_WM12A15_r2_combined GGATGTGG_79_WM12A15_r2_combined GTTCACAC_1474_WM12A15_r2_combined CTCAGAAC_1203_WM12A15_r2_combined ACGCATTC_552_WM12A15_r2_combined TAACACGT_2003_WM12A15_r2_combined CTTCTGAT_1955_WM12A15_r2_combined ACCTAGTT_225_WM12A15_r2_combined ATTCAATT_2555_WM12A15_r2_combined TGTCCATA_82_WM12A15_r2_combined AGCCCAAA_399_WM12A15_r2_combined AGAGTTCA_132_WM12A15_r2_combined TGCTTGTA_1594_WM12A15_r2_combined AGCCAGCC_2783_WM12A15_r2_combined ATCGTGGG_2852_WM12A15_r2_combined CACTTCCC_1061_WM12A15_r2_combined CTGGGGGT_258_WM12A15_r2_combined TATGTTTA_1708_WM12A15_r2_combined CACAACGC_149_WM12A15_r2_combined CCGACGCT_147_WM12A15_r2_combined ACGTGTAA_3014_WM12A15_r2_combined CTTGCCGA_1167_WM12A15_r2_combined AAGCGCTT_64_WM12A15_r2_combined TCCTTTGT_480_WM12A15_r2_combined ACTACGTT_3310_WM12A15_r2_combined ACTCAATA_1880_WM12A15_r2_combined GTAGTAAT_1316_WM12A15_r2_combined GATGCCAG_2313_WM12A15_r2_combined TTCTAGCT_1428_WM12A15_r2_combined GGTCCCTG_2987_WM12A15_r2_combined CCGCTTCT_1207_WM12A15_r2_combined CCAGCTCA_2171_WM12A15_r2_combined AGATAGCG_789_WM12A15_r2_combined GGTCGTCA_1536_WM12A15_r2_combined GAGAATGC_2825_WM12A15_r2_combined TTCGTCTC_3596_WM12A15_r2_combined CCCTGCTT_1951_WM12A15_r2_combined ACACTAGT_3597_WM12A15_r2_combined TGAATACG_905_WM12A15_r2_combined TATGTGTA_1355_WM12A15_r2_combined CCAACTAT_1025_WM12A15_r2_combined TGTGGACG_2972_WM12A15_r2_combined GTCGATCA_78_WM12A15_r2_combined CTCATGCC_184_WM12A15_r2_combined GCAGGCAA_112_WM12A15_r2_combined TCGTTAGC_2731_WM12A15_r2_combined GTGGAGTT_105_WM12A15_r2_combined TAACAGAC_50_WM12A15_r2_combined TCGGTTTG_252_WM12A15_r2_combined GCCTCAAG_2520_WM12A15_r2_combined TAATCTGA_2226_WM12A15_r2_combined TCTCGTCA_3122_WM12A15_r2_combined GCAATTTA_370_WM12A15_r2_combined CCTAATTG_1581_WM12A15_r2_combined TCCGCGAC_255_WM12A15_r2_combined AACCCCCC_1654_WM12A15_r2_combined AGAGGTCA_3310_WM12A15_r2_combined GAAGTGGC_59_WM12A15_r2_combined GGATCTTT_163_WM12A15_r2_combined TCTCGCAG_145_WM12A15_r2_combined CCGGTCCG_84_WM12A15_r2_combined CGGTTGTT_1326_WM12A15_r2_combined AAAACTAC_46_WM12A15_r2_combined AGAGTAGT_1190_WM12A15_r2_combined CCGACTTA_2078_WM12A15_r2_combined TACTTCCA_3278_WM12A15_r2_combined ATACATAC_471_WM12A15_r2_combined CCAATGTA_2603_WM12A15_r2_combined TATAAGGA_2923_WM12A15_r2_combined TCTCCGCC_1847_WM12A15_r2_combined TCACGCGA_3105_WM12A15_r2_combined GTATTGTT_2770_WM12A15_r2_combined ACGAGATT_134_WM12A15_r2_combined GTATCAGC_278_WM12A15_r2_combined GAGAGGCT_2230_WM12A15_r2_combined TTACATCA_411_WM12A15_r2_combined GCACGACA_246_WM12A15_r2_combined ACTGAAAT_125_WM12A15_r2_combined GGCATGTT_136_WM12A15_r2_combined TACTGGAT_66_WM12A15_r2_combined GAAATCAG_2874_WM12A15_r2_combined CGGATCCT_3165_WM12A15_r2_combined TCTTTTCG_2254_WM12A15_r2_combined TGGATCAG_519_WM12A15_r2_combined AAGATGTT_89_WM12A15_r2_combined CACTAGGA_1658_WM12A15_r2_combined TATCGGTA_1389_WM12A15_r2_combined TACGGTCG_2724_WM12A15_r2_combined GAAAAGCG_2966_WM12A15_r2_combined TGGTATTG_2219_WM12A15_r2_combined ATCGGCTG_525_WM12A15_r2_combined GGATTTCG_61_WM12A15_r2_combined AGTATTCA_218_WM12A15_r2_combined GTACTCCC_2616_WM12A15_r2_combined CAGCTCGG_69_WM12A15_r2_combined AAAGCCGG_77_WM12A15_r2_combined AAAAATGA_2886_WM12A15_r2_combined CCCTGGTC_3401_WM12A15_r2_combined TTATTTCT_1363_WM12A15_r2_combined GGAGGGTC_2959_WM12A15_r2_combined TTAAAATA_66_WM12A15_r2_combined TGATGTCA_219_WM12A15_r2_combined CGCGGTCC_345_WM12A15_r2_combined TGAGGCTC_2739_WM12A15_r2_combined TAATGCAG_155_WM12A15_r2_combined CCCTCAAA_2543_WM12A15_r2_combined ACTAGCTA_48_WM12A15_r2_combined TCTTATAT_1176_WM12A15_r2_combined TCACAGGG_1998_WM12A15_r2_combined AAATGTGC_2488_WM12A15_r2_combined AACATCGA_2879_WM12A15_r2_combined GTTCAACT_75_WM12A15_r2_combined AAGTGATT_342_WM12A15_r2_combined CGGCCTCT_46_WM12A15_r2_combined CCTGCGTT_963_WM12A15_r2_combined GCGCTTGA_149_WM12A15_r2_combined GCTCCATA_383_WM12A15_r2_combined TGCTACGA_62_WM12A15_r2_combined ACCCACGG_95_WM12A15_r2_combined GATCGACG_3614_WM12A15_r2_combined ACTATTAT_2579_WM12A15_r2_combined GGCCTAAT_720_WM12A15_r2_combined GTGATGTC_472_WM12A15_r2_combined TTCTCTCC_63_WM12A15_r2_combined ATTCATGA_1633_WM12A15_r2_combined TTCATGCG_1988_WM12A15_r2_combined CGGCCTAA_388_WM12A15_r2_combined CAGCCTCT_3165_WM12A15_r2_combined CACAGTGT_64_WM12A15_r2_combined AATCCGGC_271_WM12A15_r2_combined GAACTGGA_1136_WM12A15_r2_combined CAAGCGCC_54_WM12A15_r2_combined AGCTATGA_1705_WM12A15_r2_combined TGCATCCA_112_WM12A15_r2_combined GATTGATT_1739_WM12A15_r2_combined TACCCCGG_2849_WM12A15_r2_combined CGAGAGCT_2636_WM12A15_r2_combined CTTTTTGC_84_WM12A15_r2_combined ATCTTAGG_893_WM12A15_r2_combined TGATTCTA_2340_WM12A15_r2_combined AGCGGTTT_3270_WM12A15_r2_combined GTCCTGGG_1837_WM12A15_r2_combined TAGAAGTT_1325_WM12A15_r2_combined TAAACTCT_1359_WM12A15_r2_combined CTTAGTAT_864_WM12A15_r2_combined AGATCAAA_1441_WM12A15_r2_combined ACTACCCG_65_WM12A15_r2_combined GTACTAGA_57_WM12A15_r2_combined ATGTTTGC_111_WM12A15_r2_combined GGCTCTCC_3027_WM12A15_r2_combined CTCGATAA_671_WM12A15_r2_combined ATGGCTAT_73_WM12A15_r2_combined TGTCCCCT_555_WM12A15_r2_combined ACCATGGC_2254_WM12A15_r2_combined TAACTTGA_1352_WM12A15_r2_combined AGGCTTAT_3363_WM12A15_r2_combined AGAACTTC_163_WM12A15_r2_combined ATCCCGCT_521_WM12A15_r2_combined TGAGCAGA_2172_WM12A15_r2_combined TTCTCCCC_3716_WM12A15_r2_combined GGCTCGCC_115_WM12A15_r2_combined CGCCGAAG_3050_WM12A15_r2_combined GTATCAGT_3462_WM12A15_r2_combined CGGTCTAA_3075_WM12A15_r2_combined CCAGATCG_525_WM12A15_r2_combined TTCTTATA_57_WM12A15_r2_combined TTCAATCA_1021_WM12A15_r2_combined GCGTGGGT_140_WM12A15_r2_combined GTCCCTGA_249_WM12A15_r2_combined TAGTGCTT_88_WM12A15_r2_combined AAGAGCAG_522_WM12A15_r2_combined GCCCACAT_895_WM12A15_r2_combined CTATCAGG_112_WM12A15_r2_combined AGATCCGA_865_WM12A15_r2_combined ATGCTAAA_520_WM12A15_r2_combined TGTTATTA_1197_WM12A15_r2_combined GATGTGTG_3588_WM12A15_r2_combined TCCCACGA_2678_WM12A15_r2_combined CTACCGAC_2694_WM12A15_r2_combined TGAAAGGG_49_WM12A15_r2_combined ACCAGGTC_939_WM12A15_r2_combined CACAATTG_224_WM12A15_r2_combined ACTTCAAT_362_WM12A15_r2_combined ACTTTAGA_261_WM12A15_r2_combined TGGTAGGC_937_WM12A15_r2_combined AAATTAGC_429_WM12A15_r2_combined CGTCGTGG_3618_WM12A15_r2_combined AGATCCGT_2192_WM12A15_r2_combined ACCTAATT_2080_WM12A15_r2_combined TGTTAGAT_1989_WM12A15_r2_combined ACCCGCCC_3378_WM12A15_r2_combined TCACTGAT_3298_WM12A15_r2_combined GCATAAAA_3728_WM12A15_r2_combined TGTGTGCC_2864_WM12A15_r2_combined CACGGCAT_2411_WM12A15_r2_combined AACATTCG_110_WM12A15_r2_combined GGGGCTAA_3315_WM12A15_r2_combined TTCAGTTT_131_WM12A15_r2_combined GAAGACAG_3469_WM12A15_r2_combined CCCTGAGT_257_WM12A15_r2_combined GTGCGGTC_1558_WM12A15_r2_combined CCACGAGT_1647_WM12A15_r2_combined GCATTTCA_1664_WM12A15_r2_combined CGCATTTA_1111_WM12A15_r2_combined CGACCGTG_456_WM12A15_r2_combined GGCCGTCT_2676_WM12A15_r2_combined TCAAGACG_913_WM12A15_r2_combined GCCCGCAT_3324_WM12A15_r2_combined AAACCGTA_102_WM12A15_r2_combined GTACTATA_74_WM12A15_r2_combined TATATATT_173_WM12A15_r2_combined GTAGCGAC_1247_WM12A15_r2_combined CGTCCGGC_65_WM12A15_r2_combined TAGCTTGT_938_WM12A15_r2_combined ATATATAA_249_WM12A15_r2_combined GAAGAGCC_198_WM12A15_r2_combined TCCTGACT_2801_WM12A15_r2_combined TAAAGTCC_2732_WM12A15_r2_combined ACGTAGCG_962_WM12A15_r2_combined CTGTGGGT_1505_WM12A15_r2_combined AAGCGATA_59_WM12A15_r2_combined TGAAGGCT_83_WM12A15_r2_combined ATAGAAGA_3297_WM12A15_r2_combined GCAATCAT_2927_WM12A15_r2_combined CAGCTTTC_3982_WM12A15_r2_combined ACACCGGA_628_WM12A15_r2_combined TGTAGTTT_2369_WM12A15_r2_combined CAAAATTT_581_WM12A15_r2_combined AATGTATA_1031_WM12A15_r2_combined TGTTAAGC_2557_WM12A15_r2_combined TTTCACCC_2018_WM12A15_r2_combined ATATGTTG_772_WM12A15_r2_combined CCTTCGGT_159_WM12A15_r2_combined ATTGCTAT_367_WM12A15_r2_combined CGCCTAAC_3555_WM12A15_r2_combined ACAGTCCC_1223_WM12A15_r2_combined CCCCTCTC_60_WM12A15_r2_combined GTATAGTA_1899_WM12A15_r2_combined CATTCGGT_3226_WM12A15_r2_combined GCCAGGCT_566_WM12A15_r2_combined TGCGTAGT_169_WM12A15_r2_combined TGACTTAC_642_WM12A15_r2_combined AATGGGTC_502_WM12A15_r2_combined CGGACCGT_253_WM12A15_r2_combined CCTAGGTG_557_WM12A15_r2_combined AGTAAATC_1432_WM12A15_r2_combined TTGATGAG_2311_WM12A15_r2_combined TGTCTAGG_220_WM12A15_r2_combined ACAATTCT_72_WM12A15_r2_combined AAAAAAAC_243_WM12A15_r2_combined TATTGGAA_77_WM12A15_r2_combined TTAGGCCC_963_WM12A15_r2_combined TCTCGAAG_2121_WM12A15_r2_combined ACTGGTCA_1259_WM12A15_r2_combined CGCAGTTC_61_WM12A15_r2_combined TAAGGTAT_51_WM12A15_r2_combined TGGTCGAT_422_WM12A15_r2_combined AGAAAGTC_123_WM12A15_r2_combined GAAGGAAA_2420_WM12A15_r2_combined GTCCCTTC_484_WM12A15_r2_combined GGTTTCCC_265_WM12A15_r2_combined AACGATCC_1400_WM12A15_r2_combined TCTACATT_372_WM12A15_r2_combined ACCACACC_111_WM12A15_r2_combined CAACTTAT_2550_WM12A15_r2_combined ACTTGTTG_834_WM12A15_r2_combined CTACCATA_656_WM12A15_r2_combined TTTCATAC_1407_WM12A15_r2_combined GGACCTTA_159_WM12A15_r2_combined TACAACTG_1355_WM12A15_r2_combined CGACATGG_2328_WM12A15_r2_combined TCTCGTAA_2046_WM12A15_r2_combined TTCCTGAC_113_WM12A15_r2_combined GGCACTCC_2203_WM12A15_r2_combined CTATCTGA_3151_WM12A15_r2_combined TTGCAACG_107_WM12A15_r2_combined AGCCTAGT_1963_WM12A15_r2_combined GCGTGTAA_324_WM12A15_r2_combined GTATGCCG_664_WM12A15_r2_combined CCCATGAT_2521_WM12A15_r2_combined ACCCCATT_3626_WM12A15_r2_combined TAAGCAAG_2075_WM12A15_r2_combined CATCTAGG_3489_WM12A15_r2_combined TGCGAATG_257_WM12A15_r2_combined GACCAACT_3235_WM12A15_r2_combined GATTTTGT_2790_WM12A15_r2_combined CCCACAAG_271_WM12A15_r2_combined CTTGGTCC_1160_WM12A15_r2_combined GCTGTTAG_1757_WM12A15_r2_combined TATGAACT_2703_WM12A15_r2_combined CAACACTA_1713_WM12A15_r2_combined TTAGCATA_1561_WM12A15_r2_combined TCTGTCCG_2998_WM12A15_r2_combined TCCCCCAT_1481_WM12A15_r2_combined GGCATGAC_947_WM12A15_r2_combined TATCACCC_122_WM12A15_r2_combined GCGGGTAA_3336_WM12A15_r2_combined TGACACCG_2693_WM12A15_r2_combined CCGTAAGT_2696_WM12A15_r2_combined CGGGTAGT_264_WM12A15_r2_combined AGATAACG_98_WM12A15_r2_combined AGCAGGTA_135_WM12A15_r2_combined CACTCGTG_1039_WM12A15_r2_combined TCTGTTTA_3150_WM12A15_r2_combined CCAGGGTC_2998_WM12A15_r2_combined AACTGTTT_1456_WM12A15_r2_combined CCGTCTCT_775_WM12A15_r2_combined CTCCCGGC_52_WM12A15_r2_combined GCAAGGAG_3324_WM12A15_r2_combined GTAACTGT_2283_WM12A15_r2_combined GCCCGCTA_1828_WM12A15_r2_combined GGTTGCCC_3319_WM12A15_r2_combined TCTCTCCC_203_WM12A15_r2_combined CTCCGACA_736_WM12A15_r2_combined CTCTATTA_1936_WM12A15_r2_combined CCGCCACT_178_WM12A15_r2_combined TGTACGTC_61_WM12A15_r2_combined TAAGATTA_66_WM12A15_r2_combined GGCCTGAA_877_WM12A15_r2_combined TACAAGGG_2722_WM12A15_r2_combined CTGAAAGC_177_WM12A15_r2_combined GTTAGGTT_1851_WM12A15_r2_combined AGACCTAG_297_WM12A15_r2_combined AGTCGCGG_489_WM12A15_r2_combined GACTATTA_1317_WM12A15_r2_combined ATGTGCGA_1383_WM12A15_r2_combined GGCTAGGT_425_WM12A15_r2_combined CCTTCGTG_377_WM12A15_r2_combined ATTCGTAG_3144_WM12A15_r2_combined GGAAATTC_148_WM12A15_r2_combined TCACATCG_104_WM12A15_r2_combined TGGGAGTG_117_WM12A15_r2_combined GGTATCAT_1577_WM12A15_r2_combined 0.02 GAAGCCAC_11697_WM12A23_r2_combined CCTTTGTA_646_WM12A23_r2_combined TATACAAG_9934_WM12A23_r2_combined AGGGTACG_2407_WM12A23_r2_combined ACATCTCA_6966_WM12A23_r2_combined GGATTGTA_5757_WM12A23_r2_combined TCAGTCTA_198_WM12A23_r2_combined ATGTCGTA_534_WM12A23_r2_combined GCGTACGC_399_WM12A23_r2_combined AAATTTTC_6818_WM12A23_r2_combined TAGCGTTT_227_WM12A23_r2_combined ACAACGAA_577_WM12A23_r2_combined CTCTCATG_7336_WM12A23_r2_combined GCCTCCCT_6915_WM12A23_r2_combined CAATTTAC_8941_WM12A23_r2_combined CGGATGGC_1572_WM12A23_r2_combined ATCGGCTG_10323_WM12A23_r2_combined TATCTTCC_2755_WM12A23_r2_combined ATGACCCG_230_WM12A23_r2_combined TTTTAGAT_4960_WM12A23_r2_combined TGTAATCC_278_WM12A23_r2_combined CTGCTTGT_8788_WM12A23_r2_combined TACTCTCG_4220_WM12A23_r2_combined CCTTAGTT_7157_WM12A23_r2_combined TAACACGA_10239_WM12A23_r2_combined TTATTGTA_10993_WM12A23_r2_combined GTTTAGCC_8468_WM12A23_r2_combined TATTGATA_1289_WM12A23_r2_combined TCGGATAC_6998_WM12A23_r2_combined AATGTCTG_675_WM12A23_r2_combined GAAAAGCT_752_WM12A23_r2_combined AAGCGAGC_11231_WM12A23_r2_combined CACAGCTC_7610_WM12A23_r2_combined AGGGCAAA_130_WM12A23_r2_combined ACCCACTA_10543_WM12A23_r2_combined GTGTCTCC_4040_WM12A23_r2_combined TCTCGCCC_2467_WM12A23_r2_combined ACTTCAAT_299_WM12A23_r2_combined AGTGCAAA_9226_WM12A23_r2_combined TTCAAGGA_196_WM12A23_r2_combined GCGTACGT_6909_WM12A23_r2_combined AGGATTGA_1325_WM12A23_r2_combined CCGGTAAC_1212_WM12A23_r2_combined ATCCTTAT_10292_WM12A23_r2_combined TATTGCAG_9815_WM12A23_r2_combined CGTATGAC_11260_WM12A23_r2_combined TAATGGGT_12207_WM12A23_r2_combined GACGTGCC_13191_WM12A23_r2_combined TCAAGTCC_9012_WM12A23_r2_combined GCGAGACC_10752_WM12A23_r2_combined AGAAGTTT_6633_WM12A23_r2_combined CATGTAAG_8115_WM12A23_r2_combined GATCCAGT_552_WM12A23_r2_combined GTTACAGC_1708_WM12A23_r2_combined GTAGGCTT_204_WM12A23_r2_combined Week Analyzed Weeks to Viral Rebound Weeks Sample ID R1 R10 R9 R8 R7 R6 R5 R4 R3 R2 R10 R9 R8 R5 R3 R2 C. Minimum Number of Viral Lineages vs. Time to Rebound B. Sample Analyzed Compared to Rebound Occurance D. Deep Sequencing Phylogenetic Trees A. Sample and Patient information R4 R6 Sample ID PID Nadir CD4 Cell Count (categorized) CD4 at Time of Sample VL of Sample R R R R R R R R Data Not Avalible R R < Sample ID Weeks After Therapy Interuption Number of Major Lineages in Deep Sequencing Tree Minimum Number of Major Lineages in SGA Tree R0 N/A 5 5 R1 3 3 R R R R R R7 4 3 R R R Minimum 0.02 CAAGGTTG_111_WM12A24_r2_combined GAGACAGC_1801_WM12A24_r2_combined TTATCGTG_1687_WM12A24_r2_combined TATTGTAA_1355_WM12A24_r2_combined CTGGAACA_1399_WM12A24_r2_combined TACGTTTG_37_WM12A24_r2_combined CACACGTA_1577_WM12A24_r2_combined ACAGCACA_721_WM12A24_r2_combined TATCGTGT_171_WM12A24_r2_combined GTTAGCAC_1049_WM12A24_r2_combined TCAGTAAG_298_WM12A24_r2_combined AAAAAAAC_140_WM12A24_r2_combined GTATGGGA_2357_WM12A24_r2_combined GGTGAATT_1629_WM12A24_r2_combined CTTCAGGC_1444_WM12A24_r2_combined GGTGGCAA_1572_WM12A24_r2_combined TAATGTCA_178_WM12A24_r2_combined ACACCCGC_81_WM12A24_r2_combined GTTCCCGA_49_WM12A24_r2_combined GTATCATG_158_WM12A24_r2_combined CCTCGGTC_1592_WM12A24_r2_combined TCTCTCCG_1383_WM12A24_r2_combined CATTGCCA_2103_WM12A24_r2_combined TGCATAAC_1520_WM12A24_r2_combined GAAGACAT_836_WM12A24_r2_combined CCGCAGCA_1528_WM12A24_r2_combined GAGCCTAG_2191_WM12A24_r2_combined ACGGAGAT_2605_WM12A24_r2_combined AACATTTG_1419_WM12A24_r2_combined TACGCTTT_286_WM12A24_r2_combined GGTAACTA_896_WM12A24_r2_combined TACGTCGG_1501_WM12A24_r2_combined CTAAGGGG_1622_WM12A24_r2_combined GCAAGGGC_765_WM12A24_r2_combined ATCGGGTA_1525_WM12A24_r2_combined GTCTTGTC_1147_WM12A24_r2_combined TTGCAACA_1825_WM12A24_r2_combined AAACTATG_1863_WM12A24_r2_combined ATTGTATG_79_WM12A24_r2_combined GATCCTTT_1746_WM12A24_r2_combined GGTACACC_42_WM12A24_r2_combined TAAAAGTT_2762_WM12A24_r2_combined CCTTAACG_79_WM12A24_r2_combined AATAGACT_176_WM12A24_r2_combined GGCGTGAA_1670_WM12A24_r2_combined ACGGTAAA_2151_WM12A24_r2_combined GCCGTTTC_1570_WM12A24_r2_combined TAGTGCAA_2199_WM12A24_r2_combined CCCGCCAT_824_WM12A24_r2_combined TCACCCAT_368_WM12A24_r2_combined TGGCGTTC_452_WM12A24_r2_combined AGTAGGCT_2031_WM12A24_r2_combined GGTACACT_1718_WM12A24_r2_combined TCGTGTAA_1771_WM12A24_r2_combined TGGTACGA_609_WM12A24_r2_combined ATGCTAGA_984_WM12A24_r2_combined AGGTAATT_2056_WM12A24_r2_combined TCGAGCAT_1262_WM12A24_r2_combined CTTTATAC_36_WM12A24_r2_combined CGCTTAGA_1359_WM12A24_r2_combined CTTATGGA_1718_WM12A24_r2_combined CGCGAATT_504_WM12A24_r2_combined GTGAACCA_1632_WM12A24_r2_combined AAAACATC_558_WM12A24_r2_combined TCTTTTTC_1821_WM12A24_r2_combined GAGTTAAT_1760_WM12A24_r2_combined GGTATAGC_77_WM12A24_r2_combined CCCGTTAG_1092_WM12A24_r2_combined GAACGAGC_42_WM12A24_r2_combined CACCCACA_36_WM12A24_r2_combined CGTGTATA_57_WM12A24_r2_combined GTAGCTCC_1537_WM12A24_r2_combined TGACCTGC_106_WM12A24_r2_combined ACTCGATT_1324_WM12A24_r2_combined CGCGATAC_114_WM12A24_r2_combined ATCAGGGA_1496_WM12A24_r2_combined TCCTTGCC_1408_WM12A24_r2_combined AGCCTTCC_1239_WM12A24_r2_combined ATACACCC_1521_WM12A24_r2_combined TATTAGTT_88_WM12A24_r2_combined ATTTCGAG_2470_WM12A24_r2_combined GTGACGCG_1642_WM12A24_r2_combined ACACTTCC_2177_WM12A24_r2_combined GTGGGGTG_43_WM12A24_r2_combined GAGTTTAT_59_WM12A24_r2_combined TATAGGAA_309_WM12A24_r2_combined ACTATCCC_1943_WM12A24_r2_combined GCTTTGAC_1814_WM12A24_r2_combined CGGTTATT_1989_WM12A24_r2_combined TAATCTAC_1905_WM12A24_r2_combined CACTTCTG_231_WM12A24_r2_combined CGCCCCAT_2316_WM12A24_r2_combined GCCAAGCG_1877_WM12A24_r2_combined GTAAGACG_930_WM12A24_r2_combined GCCTACGG_1505_WM12A24_r2_combined CATATGTA_486_WM12A24_r2_combined GAGGCCCG_79_WM12A24_r2_combined TCCCATGG_1884_WM12A24_r2_combined TCGATATT_47_WM12A24_r2_combined AGCCGCAT_1778_WM12A24_r2_combined CGCCTCCA_1138_WM12A24_r2_combined CCACAATC_72_WM12A24_r2_combined ATGCACCC_39_WM12A24_r2_combined TATTCAAT_492_WM12A24_r2_combined CTTGGTAG_45_WM12A24_r2_combined ATAGCTGG_219_WM12A24_r2_combined AGTTCAAC_1776_WM12A24_r2_combined TAACTTTT_1471_WM12A24_r2_combined AATCCTCA_38_WM12A24_r2_combined GCTCACTG_1087_WM12A24_r2_combined CATGCGCG_1842_WM12A24_r2_combined CTTGCGCG_1403_WM12A24_r2_combined GATCTTCA_129_WM12A24_r2_combined GCTCGTAA_2068_WM12A24_r2_combined AATGGCGA_115_WM12A24_r2_combined GAGGCCGT_2461_WM12A24_r2_combined CGGCACTT_764_WM12A24_r2_combined GTAACACT_279_WM12A24_r2_combined TACCTCCC_44_WM12A24_r2_combined GCGAAACT_2041_WM12A24_r2_combined AAAGCGTA_1593_WM12A24_r2_combined CACAATAC_51_WM12A24_r2_combined GTTTGGGT_55_WM12A24_r2_combined CTACTCTC_68_WM12A24_r2_combined TACAGAAC_249_WM12A24_r2_combined CTGAAGAC_69_WM12A24_r2_combined TTAAACAT_1712_WM12A24_r2_combined ACCGTCCT_1093_WM12A24_r2_combined TTCTTCAC_1813_WM12A24_r2_combined CATCCTTC_1745_WM12A24_r2_combined CCCCTCGT_81_WM12A24_r2_combined ATGAAGCG_1145_WM12A24_r2_combined CGCCGGTA_1410_WM12A24_r2_combined TTTTCCTC_279_WM12A24_r2_combined GAGTCAGC_106_WM12A24_r2_combined CCTCGGCA_1548_WM12A24_r2_combined CTATATGC_2176_WM12A24_r2_combined AAGTTTGA_186_WM12A24_r2_combined AAAAAAAA_1878_WM12A24_r2_combined AACCGCGG_1063_WM12A24_r2_combined CTAGAATT_46_WM12A24_r2_combined CATGGAAG_1951_WM12A24_r2_combined ATATCAGT_58_WM12A24_r2_combined TATGGAGA_157_WM12A24_r2_combined AGAATATG_194_WM12A24_r2_combined GATGTAGA_2360_WM12A24_r2_combined AGGTATCC_1615_WM12A24_r2_combined ACCTCAAG_1571_WM12A24_r2_combined GTCACCGA_1858_WM12A24_r2_combined ACGTTCGG_310_WM12A24_r2_combined TTTTAGTG_1137_WM12A24_r2_combined GATGTTCG_1630_WM12A24_r2_combined TCTACATT_1089_WM12A24_r2_combined CCACAATT_59_WM12A24_r2_combined TTAATGTC_924_WM12A24_r2_combined ATCTTCGA_1251_WM12A24_r2_combined ACGCGGTA_1035_WM12A24_r2_combined GGTGTTCA_1212_WM12A24_r2_combined ATCTGTAA_1960_WM12A24_r2_combined AGTGAAGT_3_WM12A24_r2_combined GACCAAGA_54_WM12A24_r2_combined TCGCCTAA_1814_WM12A24_r2_combined GGTGGACC_1433_WM12A24_r2_combined GCGTGTTA_964_WM12A24_r2_combined CCAATGAA_72_WM12A24_r2_combined TCGGGGGA_127_WM12A24_r2_combined AGTAGAAT_169_WM12A24_r2_combined CCTGGTAT_1577_WM12A24_r2_combined GACCCATC_1817_WM12A24_r2_combined CGCTAGGG_1403_WM12A24_r2_combined CAGCCTCT_882_WM12A24_r2_combined TAATAATT_2160_WM12A24_r2_combined CACTGGTT_38_WM12A24_r2_combined CTGCAATG_715_WM12A24_r2_combined GTACACAA_1719_WM12A24_r2_combined GTAGTAGG_1441_WM12A24_r2_combined GATGTAGG_50_WM12A24_r2_combined TTGTTTCA_48_WM12A24_r2_combined ATGGACGC_2023_WM12A24_r2_combined CACCCCTA_1320_WM12A24_r2_combined GCTCGCTG_462_WM12A24_r2_combined TGAAGTCT_1825_WM12A24_r2_combined AAGCTTTT_1906_WM12A24_r2_combined TGAATTCG_56_WM12A24_r2_combined GTTTCCGA_2086_WM12A24_r2_combined TGATACGA_2127_WM12A24_r2_combined ATTGTTGA_105_WM12A24_r2_combined TTTCTTAA_43_WM12A24_r2_combined CTATATCA_1907_WM12A24_r2_combined CGTAAGTT_59_WM12A24_r2_combined CTCTGGAG_192_WM12A24_r2_combined AATGTCGA_1749_WM12A24_r2_combined GTGCAACT_69_WM12A24_r2_combined TAGTAGTG_2159_WM12A24_r2_combined TAGACTTT_438_WM12A24_r2_combined TTTCCCCG_1947_WM12A24_r2_combined TGATTAGT_2271_WM12A24_r2_combined GCGGAAAT_1270_WM12A24_r2_combined ATATCGGT_1149_WM12A24_r2_combined TAATATTA_39_WM12A24_r2_combined CCGTTACT_2012_WM12A24_r2_combined AGTTTAGT_2267_WM12A24_r2_combined ATAGTACA_939_WM12A24_r2_combined GTAGCGCC_1210_WM12A24_r2_combined TCAGGTCG_1806_WM12A24_r2_combined GAGATCTC_1924_WM12A24_r2_combined ACTCTTAA_1617_WM12A24_r2_combined TAAGTTAA_1629_WM12A24_r2_combined CATGGGAG_1532_WM12A24_r2_combined CAACGCGC_2060_WM12A24_r2_combined GGGCTTGT_2395_WM12A24_r2_combined TCTTAAGT_2303_WM12A24_r2_combined TAGCCACA_40_WM12A24_r2_combined AGCGCGTA_1084_WM12A24_r2_combined GCCATACA_2018_WM12A24_r2_combined TGGCTCGG_94_WM12A24_r2_combined GTAAAACC_1815_WM12A24_r2_combined TCAGCGGC_1832_WM12A24_r2_combined CGGTGAGG_382_WM12A24_r2_combined AGGACATT_1225_WM12A24_r2_combined TCGCAAAA_440_WM12A24_r2_combined TATATACA_212_WM12A24_r2_combined ATAAACCA_1974_WM12A24_r2_combined AACGAACG_42_WM12A24_r2_combined CATAGTAC_286_WM12A24_r2_combined AGTTTGTG_2392_WM12A24_r2_combined GACAAAGA_2327_WM12A24_r2_combined AGTAACAA_2078_WM12A24_r2_combined TAATACAA_52_WM12A24_r2_combined CAACGGCT_787_WM12A24_r2_combined TCAAGCTA_1897_WM12A24_r2_combined CGCTAAAT_988_WM12A24_r2_combined GGTTACGA_2450_WM12A24_r2_combined CACGCATT_1236_WM12A24_r2_combined TTTGGGTG_2643_WM12A24_r2_combined TGGCGAAT_85_WM12A24_r2_combined CACTTTAC_1122_WM12A24_r2_combined TCCGACAA_85_WM12A24_r2_combined ACTTGGTT_52_WM12A24_r2_combined GATTTCGA_1962_WM12A24_r2_combined CTCAACGC_1923_WM12A24_r2_combined CAGGCCCT_1395_WM12A24_r2_combined TATATATC_2123_WM12A24_r2_combined TTCCTTGT_39_WM12A24_r2_combined AAGATCAT_903_WM12A24_r2_combined CACTACAG_477_WM12A24_r2_combined CCTCAGAT_2009_WM12A24_r2_combined CGGCCTCT_68_WM12A24_r2_combined GAAGGTCC_358_WM12A24_r2_combined GTCAACTC_943_WM12A24_r2_combined CCAGAATA_1051_WM12A24_r2_combined CATAGCAC_1518_WM12A24_r2_combined CAGAGTTA_2042_WM12A24_r2_combined TTACCCCT_1776_WM12A24_r2_combined AGTCTTGA_1975_WM12A24_r2_combined TTCCTCAG_1795_WM12A24_r2_combined TACATAAT_1891_WM12A24_r2_combined CGATGTAG_1095_WM12A24_r2_combined ACTATCGC_1967_WM12A24_r2_combined CACTGATT_2125_WM12A24_r2_combined TAGTTGTG_1398_WM12A24_r2_combined ATTAGCAG_1621_WM12A24_r2_combined TAGGCTCT_1721_WM12A24_r2_combined CATAATAC_1729_WM12A24_r2_combined CAAAAGAA_2368_WM12A24_r2_combined GAGAGGAA_133_WM12A24_r2_combined TATCGTAG_2269_WM12A24_r2_combined GAAAGCAC_44_WM12A24_r2_combined CAAGTGCG_1019_WM12A24_r2_combined GGGGTGCC_1928_WM12A24_r2_combined AATCTACG_1808_WM12A24_r2_combined GGGTGTAG_2008_WM12A24_r2_combined AGTGTGGG_44_WM12A24_r2_combined GGAACCGC_1787_WM12A24_r2_combined TCATCTTT_1103_WM12A24_r2_combined TTATTATA_2112_WM12A24_r2_combined 0.02 CAACACCT_151_WM12A22_r2_combined ACGTCGGC_4010_WM12A22_r2_combined CTTCATAG_3461_WM12A22_r2_combined CTTGATTG_2221_WM12A22_r2_combined TCAGTTTT_3551_WM12A22_r2_combined AAAAGTAG_251_WM12A22_r2_combined GCTGTTGT_2744_WM12A22_r2_combined TAAGTTTC_424_WM12A22_r2_combined CCTCGGAC_4345_WM12A22_r2_combined GTCCAATT_3557_WM12A22_r2_combined CGGGCGCC_333_WM12A22_r2_combined CCCACTCA_3660_WM12A22_r2_combined TGGTGTAT_4716_WM12A22_r2_combined GGAACATG_3694_WM12A22_r2_combined TCAGACGG_3883_WM12A22_r2_combined GGAGGAAC_4529_WM12A22_r2_combined AAATAAGA_246_WM12A22_r2_combined AGGAGCCT_295_WM12A22_r2_combined AAAGTTCG_67_WM12A22_r2_combined ATGTCTAG_2282_WM12A22_r2_combined GAGCTGAT_3785_WM12A22_r2_combined TTTAACGT_2353_WM12A22_r2_combined AAATCATC_2556_WM12A22_r2_combined TCAAAGCC_245_WM12A22_r2_combined CTCCCAAA_81_WM12A22_r2_combined GATGATTG_190_WM12A22_r2_combined GCATGTAA_443_WM12A22_r2_combined TATAGGTA_3513_WM12A22_r2_combined ACATCTGT_871_WM12A22_r2_combined CCCTTGCG_3075_WM12A22_r2_combined AAGATGAT_130_WM12A22_r2_combined ACCATACC_3603_WM12A22_r2_combined GGTTCTGC_93_WM12A22_r2_combined AAGATAAT_3944_WM12A22_r2_combined TCGTTGTG_70_WM12A22_r2_combined AGCGTTTA_3446_WM12A22_r2_combined GCGATAAT_133_WM12A22_r2_combined CCGGTCAG_3117_WM12A22_r2_combined TAAGATTC_65_WM12A22_r2_combined TTGCGTTT_3379_WM12A22_r2_combined GATTGAAT_2213_WM12A22_r2_combined AGGGCGCT_4595_WM12A22_r2_combined CCGGGTGT_299_WM12A22_r2_combined GGCGAGGA_78_WM12A22_r2_combined TCCCGTCA_1502_WM12A22_r2_combined GTGTCATA_3738_WM12A22_r2_combined CGATCCGA_3914_WM12A22_r2_combined GGACTAAC_1517_WM12A22_r2_combined CAATGCCG_2683_WM12A22_r2_combined TTATGGTG_104_WM12A22_r2_combined GGCAGTGC_65_WM12A22_r2_combined GTGAGTTT_2689_WM12A22_r2_combined CTAGACAA_3817_WM12A22_r2_combined ATCGATCT_92_WM12A22_r2_combined TCTATTTT_276_WM12A22_r2_combined CTAAGGTT_4143_WM12A22_r2_combined CAACCGTG_3394_WM12A22_r2_combined CATTAGGG_122_WM12A22_r2_combined AGCTTTCA_107_WM12A22_r2_combined TTCAGCGT_127_WM12A22_r2_combined GAGAGAAC_3975_WM12A22_r2_combined TAGATTTA_3445_WM12A22_r2_combined TTGTCCTT_3671_WM12A22_r2_combined CGACAAAT_4465_WM12A22_r2_combined ACAAGCTC_3326_WM12A22_r2_combined GAATCATC_3881_WM12A22_r2_combined CTTCCAAA_1508_WM12A22_r2_combined CGAATCTA_268_WM12A22_r2_combined TATCATAG_3889_WM12A22_r2_combined ACTGGCCC_3929_WM12A22_r2_combined GCTGTCTT_4027_WM12A22_r2_combined CCTTTCCT_71_WM12A22_r2_combined ACCAGTGG_4628_WM12A22_r2_combined GAAAGGAG_3034_WM12A22_r2_combined CCCAGGGA_206_WM12A22_r2_combined TGACGCTG_136_WM12A22_r2_combined GGTGCATG_4202_WM12A22_r2_combined TGCGGAAC_96_WM12A22_r2_combined TTACGCTA_4550_WM12A22_r2_combined GGCTTGCC_4124_WM12A22_r2_combined TTTACTTA_93_WM12A22_r2_combined GGTCAGCA_549_WM12A22_r2_combined TAACAGGG_3904_WM12A22_r2_combined CGGCGACC_148_WM12A22_r2_combined GGTCAAGA_3439_WM12A22_r2_combined ATGAGCCT_125_WM12A22_r2_combined AGGCCCCA WM12A22_r2_combined TATTGCTT_3221_WM12A22_r2_combined AGAGATAG_3882_WM12A22_r2_combined GTTCTAGA_128_WM12A22_r2_combined CTGTAAAT_2193_WM12A22_r2_combined TTGTTCTT_69_WM12A22_r2_combined TATGAGTT_1740_WM12A22_r2_combined CTGTATTG_79_WM12A22_r2_combined ACATTTTG_3085_WM12A22_r2_combined TTTTTCTG_3007_WM12A22_r2_combined GTCTTAAC_3247_WM12A22_r2_combined ATAAGTCC_89_WM12A22_r2_combined GTGCTATC_3347_WM12A22_r2_combined ATCCAGTA_4147_WM12A22_r2_combined AGATCTAA_69_WM12A22_r2_combined ACCTTTCA_2505_WM12A22_r2_combined GCCGAGAA_5015_WM12A22_r2_combined ACTGACTC_56_WM12A22_r2_combined CACTTAGT_63_WM12A22_r2_combined CTCGATAT_4484_WM12A22_r2_combined CACAGGTA_882_WM12A22_r2_combined CAAAGAGG_3828_WM12A22_r2_combined TGATTGCC_3795_WM12A22_r2_combined AAACCAAG_3985_WM12A22_r2_combined AAACAGAC_1191_WM12A22_r2_combined GTTTTGGA_636_WM12A22_r2_combined GGGAGTCT_68_WM12A22_r2_combined CAGCGGGG_3590_WM12A22_r2_combined GTAAGTTA_4034_WM12A22_r2_combined GGACTTAT_3375_WM12A22_r2_combined AAGGACCT_3401_WM12A22_r2_combined CCTGCCGG_3877_WM12A22_r2_combined AAAATGTA_273_WM12A22_r2_combined TACATTAG_3830_WM12A22_r2_combined TCACCAAC_3568_WM12A22_r2_combined AGCATCAC_1833_WM12A22_r2_combined GTCACAAG_3406_WM12A22_r2_combined CCAGTTAT_87_WM12A22_r2_combined TCATTACG_2689_WM12A22_r2_combined GATAAGGC_4741_WM12A22_r2_combined GATGTTGT_298_WM12A22_r2_combined AAGTGACC_325_WM12A22_r2_combined ATCCAAAA_3394_WM12A22_r2_combined GGTCGTCC_4057_WM12A22_r2_combined ACCAGCTG_3319_WM12A22_r2_combined TCCAGCAA_2324_WM12A22_r2_combined ATTGTCCA_3675_WM12A22_r2_combined GCTTGGTG_3683_WM12A22_r2_combinedCGTCGAAA_3691_WM12A22_r2_combined AGCCGAGG_3688_WM12A22_r2_combined ACGTAGTA_1658_WM12A22_r2_combined GGTTTTAC_2074_WM12A22_r2_combined AACCGGAA_95_WM12A22_r2_combined CGTTCCGA_3467_WM12A22_r2_combined GCTCATGG_4480_WM12A22_r2_combined AATTGCCA_2576_WM12A22_r2_combined AGTTCCGC_2471_WM12A22_r2_combined CATGTTCA_3174_WM12A22_r2_combined AAGTCATG_2721_WM12A22_r2_combined AGACTTGT_565_WM12A22_r2_combined CATACCGA_3116_WM12A22_r2_combined TCTGGAAA_572_WM12A22_r2_combined ATGCTCTT_2642_WM12A22_r2_combined TCCCTTGT_86_WM12A22_r2_combined ATTTACTA_2218_WM12A22_r2_combined CTGTATCG_1959_WM12A22_r2_combined CCTTCACA_2607_WM12A22_r2_combined GGGACCAG_298_WM12A22_r2_combined TGAGACGA_4332_WM12A22_r2_combined TTCAGCAC_2488_WM12A22_r2_combined TGACAGGG_56_WM12A22_r2_combined GCTAGGCA_4389_WM12A22_r2_combined ATGACGTT_4245_WM12A22_r2_combined GCACTCGA_275_WM12A22_r2_combined CCAGACAA_96_WM12A22_r2_combined TCGTGCTC_3392_WM12A22_r2_combined AGGCTTTC_133_WM12A22_r2_combined GCTAGGGG_666_WM12A22_r2_combined 0.02 GTAGCAAT_166_WM12A20_r2_combined CTCGGTAT_1261_WM12A20_r2_combined CATCAGTT_1172_WM12A20_r2_combined GAAACCCT_722_WM12A20_r2_combined GAGGTCGT_1616_WM12A20_r2_combined TAGGCTTG_1308_WM12A20_r2_combined ACTGGCAT_45_WM12A20_r2_combined GTATCAAT_1291_WM12A20_r2_combined GCCATTTA_1285_WM12A20_r2_combined CTGAAACT_1359_WM12A20_r2_combined ATGTGGAA_35_WM12A20_r2_combined TGGGCAAA_1445_WM12A20_r2_combined TTTTTAGC_29_WM12A20_r2_combined CCCAACCG_35_WM12A20_r2_combined GTCTGTCC_952_WM12A20_r2_combined ACATAAAC_34_WM12A20_r2_combined TGTCTGGG_1639_WM12A20_r2_combined TTTGTAAT_843_WM12A20_r2_combined ACCAGTTA_47_WM12A20_r2_combined AACCTGCC_124_WM12A20_r2_combined ATTTCCAC_1151_WM12A20_r2_combined CCTATTTC_1159_WM12A20_r2_combined CTCGGAGT_64_WM12A20_r2_combined AGTCTCGC_1434_WM12A20_r2_combined ACTGTTAC_333_WM12A20_r2_combined AAAGGGGG_1610_WM12A20_r2_combined AATCACAT_1419_WM12A20_r2_combined ATGAGTAA_1155_WM12A20_r2_combined ACACGGTA_1405_WM12A20_r2_combined ATCAGCAG_1477_WM12A20_r2_combined CATGGGAA_1257_WM12A20_r2_combined GTGACAAC_29_WM12A20_r2_combined ATGATGTC_1540_WM12A20_r2_combined GCCGGGTT_28_WM12A20_r2_combined AAAATCAC_1416_WM12A20_r2_combined TTATTTAT_1459_WM12A20_r2_combined AGGGTACA_647_WM12A20_r2_combined CATTTACA_1154_WM12A20_r2_combined AGGCGTAT_1376_WM12A20_r2_combined CCCCACCT_39_WM12A20_r2_combined TAACTGCC_1565_WM12A20_r2_combined TTAGGTGC_1447_WM12A20_r2_combined GACCAGCA_951_WM12A20_r2_combined GGGATTTG_1361_WM12A20_r2_combined ATGTTCCT_1623_WM12A20_r2_combined GCGAATCG_161_WM12A20_r2_combined ACTCTGCG_1639_WM12A20_r2_combined TGTTCACC_951_WM12A20_r2_combined GGGGAAAA_1644_WM12A20_r2_combined CTGCAGGC_30_WM12A20_r2_combined GTCTTAGT_623_WM12A20_r2_combined TGGGCAAG_1464_WM12A20_r2_combined ACGGGAGT_1206_WM12A20_r2_combined GAGGAAAA_1270_WM12A20_r2_combined CTATCAGC_1379_WM12A20_r2_combined GCGTGCGA_106_WM12A20_r2_combined TGCTGTGT_1434_WM12A20_r2_combined TCTACCCA_811_WM12A20_r2_combined ACGAAAAG_1244_WM12A20_r2_combined GGTTTCTC_967_WM12A20_r2_combined GAGTAGAA_1179_WM12A20_r2_combined CCCAAAGC_1746_WM12A20_r2_combined GGGTATTG_1198_WM12A20_r2_combined CCTATTCC_30_WM12A20_r2_combined GCCAAATG_808_WM12A20_r2_combined GGGCCACT_1295_WM12A20_r2_combined GTAATGCT_1115_WM12A20_r2_combined AGCCCGAC_32_WM12A20_r2_combined ATGACAAC_1252_WM12A20_r2_combined TTTGGGGT_515_WM12A20_r2_combined CGTCATGC_1249_WM12A20_r2_combined TCATTTTC_1141_WM12A20_r2_combined CCATAACG_74_WM12A20_r2_combined TCATCCCA_1518_WM12A20_r2_combined AAGTTGAA_1551_WM12A20_r2_combined GCCCTTGC_1236_WM12A20_r2_combined GATAAAGC_1456_WM12A20_r2_combined GTGAGTCA_80_WM12A20_r2_combined ATGCAGTT_46_WM12A20_r2_combined GTGTGATA_1525_WM12A20_r2_combined TTACAGAA_615_WM12A20_r2_combined ATAAGTGC_1396_WM12A20_r2_combined ACCAGCAT_30_WM12A20_r2_combined GCCATAGT_1144_WM12A20_r2_combined CCGGGTTA_1268_WM12A20_r2_combined GGGATCCA_1387_WM12A20_r2_combined TGAGGCTT_1041_WM12A20_r2_combined TCAACTTT_1109_WM12A20_r2_combined TCTATCTT_1291_WM12A20_r2_combined GAATAATT_87_WM12A20_r2_combined TGAGGAGT_1583_WM12A20_r2_combined ACTTGCAC_41_WM12A20_r2_combined TTCGACTC_42_WM12A20_r2_combined ATTATGTT_1085_WM12A20_r2_combined TGTGCCTC_601_WM12A20_r2_combined GGCCTAGT_30_WM12A20_r2_combined CAGGCGCG_39_WM12A20_r2_combined GACAAAAC_1251_WM12A20_r2_combined GTTTAAGG_804_WM12A20_r2_combined AAAAGATC_1526_WM12A20_r2_combined AAGCACGC_1306_WM12A20_r2_combined TCTCCTAT_53_WM12A20_r2_combined ATGCTATT_1366_WM12A20_r2_combined CCTGGCGG_1463_WM12A20_r2_combined GACGTAGA_48_WM12A20_r2_combined ACATCAGA_1201_WM12A20_r2_combined TTTCGGCG_1058_WM12A20_r2_combined CGCCCCCA_1595_WM12A20_r2_combined GCCCAGTA_1247_WM12A20_r2_combined CCAAATCA_28_WM12A20_r2_combined TAACAGTT_1135_WM12A20_r2_combined CAGGACAT_38_WM12A20_r2_combined ACTGGTAC_1065_WM12A20_r2_combined CTCATGTA_1060_WM12A20_r2_combined TAGGGTAC_52_WM12A20_r2_combined TGCGTTAG_717_WM12A20_r2_combined ATCGTCAT_1372_WM12A20_r2_combined CAGAACAT_1319_WM12A20_r2_combined GACATGGC_1153_WM12A20_r2_combined TTCCTAGC_1228_WM12A20_r2_combined CTGACTTC_1282_WM12A20_r2_combined GTTCTGCG_335_WM12A20_r2_combined TCGATTTG_1163_WM12A20_r2_combined GCCCGAGC_1426_WM12A20_r2_combined TTGCTGAG_1027_WM12A20_r2_combined GCGGCCCT_1304_WM12A20_r2_combined CCAAGGGC_1131_WM12A20_r2_combined AACAGCCA_1368_WM12A20_r2_combined TAATAAGA_1602_WM12A20_r2_combined TTGTACTA_1101_WM12A20_r2_combined GTACCTCA_158_WM12A20_r2_combined CGGGAGGG_76_WM12A20_r2_combined TCAACCGT_1073_WM12A20_r2_combined ACTAGCGT_151_WM12A20_r2_combined TTCATAAC_57_WM12A20_r2_combined AGCGTACA_1092_WM12A20_r2_combined GCTAATTC_1462_WM12A20_r2_combined CCATTCAT_1503_WM12A20_r2_combined CAGTAATC_42_WM12A20_r2_combined CTGTTACA_1040_WM12A20_r2_combined ATTAGAGC_1492_WM12A20_r2_combined TTTAAAGG_1274_WM12A20_r2_combined CTCAACCG_1475_WM12A20_r2_combined TTAATCAT_1209_WM12A20_r2_combined GAGCCATG_34_WM12A20_r2_combined CATCCTAT_49_WM12A20_r2_combined GATCTGAA_136_WM12A20_r2_combined TCGTACGG_98_WM12A20_r2_combined TCCACGAT_41_WM12A20_r2_combined CCCGGCCC_1343_WM12A20_r2_combined GAGCCGGC_64_WM12A20_r2_combined TCATGTGT_434_WM12A20_r2_combined AGACGCTG_29_WM12A20_r2_combined GAAACGAG_1165_WM12A20_r2_combined GGAGTCAG_1199_WM12A20_r2_combined TTTACCTT_50_WM12A20_r2_combined CTCTCGGT_1526_WM12A20_r2_combined GGGGCAAG_1396_WM12A20_r2_combined CTCCCCTT_671_WM12A20_r2_combined CCACTGTA_31_WM12A20_r2_combined TGTTAAGA_1593_WM12A20_r2_combined CATGACCG_708_WM12A20_r2_combined ATCAAGGG_70_WM12A20_r2_combined ATTCGGCC_48_WM12A20_r2_combined GTACATTA_1411_WM12A20_r2_combined TCAAACTT_1519_WM12A20_r2_combined GCTTTAAC_32_WM12A20_r2_combined CCGGACGC_1276_WM12A20_r2_combined AACAAGAG_1176_WM12A20_r2_combined CGTGACCA_1474_WM12A20_r2_combined GCAACCCC_549_WM12A20_r2_combined TTAGCTTG_54_WM12A20_r2_combined CACTCAAC_1647_WM12A20_r2_combined AGTCGTAT_946_WM12A20_r2_combined TGTCGAGG_2060_WM12A20_r2_combined CCGACCTT_1360_WM12A20_r2_combined TTGGAGGA_1220_WM12A20_r2_combined ATGATTTT_89_WM12A20_r2_combined CAGGTGGA_1340_WM12A20_r2_combined CAGATCAA_1360_WM12A20_r2_combined TTCGTCAC_209_WM12A20_r2_combined ACTGCATA_1275_WM12A20_r2_combined TGACCAAG_249_WM12A20_r2_combined TCCATAGT_1502_WM12A20_r2_combined AAGTACCT_1501_WM12A20_r2_combined TAGCAGCT_1269_WM12A20_r2_combined GCGTCACA_1492_WM12A20_r2_combined TGTGCCCA_626_WM12A20_r2_combined AGATAAGG_1341_WM12A20_r2_combined TTCGCGCT_578_WM12A20_r2_combined TATTGGTA_37_WM12A20_r2_combined TTTTAGTA_78_WM12A20_r2_combined ATTCTAAA_696_WM12A20_r2_combined ATCGCACC_75_WM12A20_r2_combined CATCGATT_1544_WM12A20_r2_combined AGATATCA_848_WM12A20_r2_combined CTAAGTAA_1354_WM12A20_r2_combined ATAAACAA_565_WM12A20_r2_combined TGTCGTTT_1118_WM12A20_r2_combined CTCGGGTG_1384_WM12A20_r2_combined CCCGGTCG_1353_WM12A20_r2_combined CGAAATTA_643_WM12A20_r2_combined TGCAATCG_1323_WM12A20_r2_combined CATGCAGA_1155_WM12A20_r2_combined ACTCATTG_661_WM12A20_r2_combined TTTACGGG_1400_WM12A20_r2_combined AATTTGGT_1561_WM12A20_r2_combined CTCAGTGA_1517_WM12A20_r2_combined CATGTGTC_1331_WM12A20_r2_combined TTCGGTAT_109_WM12A20_r2_combined GGGGCGAT_47_WM12A20_r2_combined TGTTTAGA_299_WM12A20_r2_combined CGCAGGTA_49_WM12A20_r2_combined AGGTGCTG_1505_WM12A20_r2_combined CTATTTTT_664_WM12A20_r2_combined GCCTCCCT_1961_WM12A20_r2_combined TACTACCT_1155_WM12A20_r2_combined TTTTCGTG_61_WM12A20_r2_combined CCGCGCTG_28_WM12A20_r2_combined TTGTACTG_1341_WM12A20_r2_combined CAATTCTG_975_WM12A20_r2_combined ATCGTACT_1213_WM12A20_r2_combined AACCTGTC_852_WM12A20_r2_combined CCCCCCGT_997_WM12A20_r2_combined TGAAAGAT_989_WM12A20_r2_combined GCATCTCT_642_WM12A20_r2_combined TGGGCGAT_1235_WM12A20_r2_combined GTCGGCCA_789_WM12A20_r2_combined GGGTTGAA_1044_WM12A20_r2_combined CTAATAGT_34_WM12A20_r2_combined TGTTCAAT_1536_WM12A20_r2_combined GAGCCTCT_49_WM12A20_r2_combined GATGTAGA_1340_WM12A20_r2_combined CTCAGGTA_676_WM12A20_r2_combined ATCCACGC_792_WM12A20_r2_combined ACTCAACC_31_WM12A20_r2_combined GTAGCTAC_1580_WM12A20_r2_combined AATCTTGC_1436_WM12A20_r2_combined ATTCCTAG_1622_WM12A20_r2_combined GCCTTAGG_117_WM12A20_r2_combined AGCAGCTA_1438_WM12A20_r2_combined GAGGTCGC_1094_WM12A20_r2_combined TAGCCCGC_32_WM12A20_r2_combined CGCACTGG_113_WM12A20_r2_combined ATGCCCTG_816_WM12A20_r2_combined CAACACTT_1167_WM12A20_r2_combined CACCTAGG_1468_WM12A20_r2_combined AAGACTTA_1410_WM12A20_r2_combined AGACCCCC_53_WM12A20_r2_combined ACCCCTTA_395_WM12A20_r2_combined GTGACTGT_1731_WM12A20_r2_combined AGTTGTGA_946_WM12A20_r2_combined TTCACTGG_195_WM12A20_r2_combined GGACCGAA_46_WM12A20_r2_combined CCGCGCTA_1180_WM12A20_r2_combined ATGGCGTT_1736_WM12A20_r2_combined GGTCAGTT_1247_WM12A20_r2_combined GAACAAAT_548_WM12A20_r2_combined ATGGTTCC_667_WM12A20_r2_combined GTTCTCCT_429_WM12A20_r2_combined GTTCTCTA_1349_WM12A20_r2_combined GCCCTAAG_1173_WM12A20_r2_combined CGAAAAGC_167_WM12A20_r2_combined TAATTCCG_1031_WM12A20_r2_combined ATCCCCTT_31_WM12A20_r2_combinedGATGATAT_1268_WM12A20_r2_combined GCGTACAA_1097_WM12A20_r2_combined ATTGGTTC_1370_WM12A20_r2_combined CTTGGTCG_667_WM12A20_r2_combined GGTTAACC_1178_WM12A20_r2_combined TAAGCATG_1103_WM12A20_r2_combined TCGGGAGG_588_WM12A20_r2_combined TGCGTGCC_1713_WM12A20_r2_combined AGTATTTC_1226_WM12A20_r2_combined AGCGGCGG_1077_WM12A20_r2_combined TCAGATTT_87_WM12A20_r2_combined TTGCTTTC_109_WM12A20_r2_combined AGTATCCC_1480_WM12A20_r2_combined CGCGTACA_1089_WM12A20_r2_combined TTTTCCTT_971_WM12A20_r2_combined TTACGAGT_757_WM12A20_r2_combined GTTCGTTA_1591_WM12A20_r2_combined GTATCGAC_69_WM12A20_r2_combined TCGCTGTC_1384_WM12A20_r2_combined GAGTGCGA_1672_WM12A20_r2_combined GGACTTAT_1315_WM12A20_r2_combined AGGTAGTA_840_WM12A20_r2_combined TCTCAAAG_76_WM12A20_r2_combined GTACTCAA_921_WM12A20_r2_combined TGCATTCT_137_WM12A20_r2_combined CGAAGTTT_1299_WM12A20_r2_combined TTATGGGA_1440_WM12A20_r2_combined TTATAGTT_1505_WM12A20_r2_combined TATGGCGG_1572_WM12A20_r2_combined CACAGTGC_1269_WM12A20_r2_combined TCCCAAGT_218_WM12A20_r2_combined TTTCTTTT_1181_WM12A20_r2_combined AAATGACT_1232_WM12A20_r2_combined CGTTTTCT_1361_WM12A20_r2_combined AATTGAAT_136_WM12A20_r2_combined AGTCCCGC_76_WM12A20_r2_combinedGTAGGTTC_1033_WM12A20_r2_combined AGCCTCGT_973_WM12A20_r2_combined GTATGATT_980_WM12A20_r2_combined AGCTGCAC_1277_WM12A20_r2_combined ATGGGACC_1485_WM12A20_r2_combined TCCTAGGA_892_WM12A20_r2_combined AGTGATAG_1347_WM12A20_r2_combined CAAAAACC_101_WM12A20_r2_combined TATACACG_107_WM12A20_r2_combined TTGGCCGC_34_WM12A20_r2_combined AGCAAGCT_70_WM12A20_r2_combined GGGGGCTG_46_WM12A20_r2_combined ATGCATGC_1044_WM12A20_r2_combined CTCTGGGA_240_WM12A20_r2_combined CTTTAGGC_114_WM12A20_r2_combined CGGGCCTA_1524_WM12A20_r2_combined TTCATTAA_1316_WM12A20_r2_combined TTGAGCTT_1427_WM12A20_r2_combined CTGACACT_991_WM12A20_r2_combined TATGGTGA_58_WM12A20_r2_combined ATTGTACC_936_WM12A20_r2_combined TGCTTGTG_1409_WM12A20_r2_combined CACGCTAT_1429_WM12A20_r2_combined TTTATGTT_84_WM12A20_r2_combined AGCTACGG_38_WM12A20_r2_combined CTGCGTTG_952_WM12A20_r2_combined CGGGACGA_1170_WM12A20_r2_combined TTTTTTGA_639_WM12A20_r2_combined TTAGAGGG_1785_WM12A20_r2_combined AGCCCGAT_28_WM12A20_r2_combined GTCCGCTT_1549_WM12A20_r2_combined CCTGGTGA_725_WM12A20_r2_combined GAAGCAGC_1375_WM12A20_r2_combined AACCATAC_977_WM12A20_r2_combined CGCCGATT_394_WM12A20_r2_combined ATGACCCT_1124_WM12A20_r2_combined GCGTACGA_76_WM12A20_r2_combined TGGCTGGT_1273_WM12A20_r2_combined ATGGTAAT_1439_WM12A20_r2_combined AGTCCTCG_1400_WM12A20_r2_combined GAAAAATG_1545_WM12A20_r2_combined ACTAGCAT_428_WM12A20_r2_combined TACGTCCT_93_WM12A20_r2_combined ACAAGTTG_682_WM12A20_r2_combined GGACGAAC_58_WM12A20_r2_combined AAAGGATC_71_WM12A20_r2_combined ATAGCGTA_1448_WM12A20_r2_combined CTAACCGA_45_WM12A20_r2_combined TATTACCT_1667_WM12A20_r2_combined GCGTTACT_630_WM12A20_r2_combined ATCGTGAC_1483_WM12A20_r2_combined TAGCCAAT_1659_WM12A20_r2_combined TGTGTCGT_1212_WM12A20_r2_combined ACAACCGT_920_WM12A20_r2_combined AATATTTC_214_WM12A20_r2_combined ATATGAAT_752_WM12A20_r2_combined CAAACCTC_1433_WM12A20_r2_combined TGCTTACT_923_WM12A20_r2_combined ATAAGATT_949_WM12A20_r2_combined GAGTATGT_118_WM12A20_r2_combined GGTCCACT_167_WM12A20_r2_combined CACCCAGC_31_WM12A20_r2_combined ATGAGGAT_94_WM12A20_r2_combined ATTTTTTA_1216_WM12A20_r2_combined ATTGCGAG_1429_WM12A20_r2_combined AAACAAAT_31_WM12A20_r2_combined GCTTAACG_1576_WM12A20_r2_combined ACGACCGT_38_WM12A20_r2_combined GACGGTAA_438_WM12A20_r2_combined CACGTGTG_1590_WM12A20_r2_combined GCCATAGG_440_WM12A20_r2_combined TATAATCT_1441_WM12A20_r2_combined CCGACGCG_1420_WM12A20_r2_combined AGTATTCT_1126_WM12A20_r2_combined TGCAAGCC_1474_WM12A20_r2_combined ACCGGGGG_1124_WM12A20_r2_combined AACGTGGG_1749_WM12A20_r2_combined CTATCGTA_775_WM12A20_r2_combined ATACGATC_1426_WM12A20_r2_combined CATATTTG_1378_WM12A20_r2_combined CTGCAGAT_50_WM12A20_r2_combined AATATAGC_1028_WM12A20_r2_combined CCTACCAG_1468_WM12A20_r2_combined GCCCTAGT_38_WM12A20_r2_combined GCTGCCAC_1475_WM12A20_r2_combined 0.02 ATTCCTCA_1359_WM12A21_r2_combined TTTAGATT_969_WM12A21_r2_combined TCCACATA_1171_WM12A21_r2_combined CCATCCTC_830_WM12A21_r2_combinedCCACAAGT_1170_WM12A21_r2_combined AGTAGGAG_1574_WM12A21_r2_combined ATGGTCCG_1392_WM12A21_r2_combined ATATAATT_1746_WM12A21_r2_combined CACACACT_71_WM12A21_r2_combined GATATAAA_1720_WM12A21_r2_combined ACCATGTC_1396_WM12A21_r2_combined TTATGCTG_1311_WM12A21_r2_combined TTCCATGA_1540_WM12A21_r2_combined TATAACGG_995_WM12A21_r2_combined TTAAGCTC_1296_WM12A21_r2_combined GAACCGTT_234_WM12A21_r2_combined AGTACATT_41_WM12A21_r2_combined GACAGTCC_1238_WM12A21_r2_combined ATTACCAT_1351_WM12A21_r2_combined AGTAACCG_1396_WM12A21_r2_combined AATTTAAC_42_WM12A21_r2_combined TTTTACAG_475_WM12A21_r2_combined GCCGCAAA_1039_WM12A21_r2_combined GACAATTG_1666_WM12A21_r2_combined TGTATTTT_70_WM12A21_r2_combined CACTCTCA_482_WM12A21_r2_combined GTTCGTCA_1420_WM12A21_r2_combined AGCAGAGA_1373_WM12A21_r2_combined TTTAGGGG_135_WM12A21_r2_combined GCTTGCGA_1065_WM12A21_r2_combined CAGAGTGT_1581_WM12A21_r2_combined GTTCCTTG_1621_WM12A21_r2_combined TGCTGCCA_1465_WM12A21_r2_combined TTTAGCTT_1620_WM12A21_r2_combined ATAGGACG_426_WM12A21_r2_combined AGGTGGTC_1242_WM12A21_r2_combined GGGGAGGG_37_WM12A21_r2_combined TTGTCTGA_76_WM12A21_r2_combined CACCCTTA_1262_WM12A21_r2_combined CTGAGTGT_48_WM12A21_r2_combined TCTCATTG_647_WM12A21_r2_combined GTGAGAAT_1016_WM12A21_r2_combined CGCCATCT_1585_WM12A21_r2_combined AGTACGGA_1386_WM12A21_r2_combined AATATCAA_867_WM12A21_r2_combined GCAATAGG_204_WM12A21_r2_combined TGCAGCCG_1430_WM12A21_r2_combined AACAAGGA_1644_WM12A21_r2_combined GTATGTAG_1110_WM12A21_r2_combined TGTTACAA_1520_WM12A21_r2_combined TGGAGACA_234_WM12A21_r2_combined TCATTGTG_1149_WM12A21_r2_combined GGCACTCC_132_WM12A21_r2_combined GTACTTGT_1241_WM12A21_r2_combined GGAACTGC_1320_WM12A21_r2_combined TTTTAGCG_1635_WM12A21_r2_combined ATAATATG_1436_WM12A21_r2_combined AAACAAGG_541_WM12A21_r2_combined TTGGCTGC_1235_WM12A21_r2_combined GGGACAAC_1210_WM12A21_r2_combined TGTATGGT_1648_WM12A21_r2_combined GAGGGCTT_945_WM12A21_r2_combined ATATATAT_1087_WM12A21_r2_combined AGGAAAAC_75_WM12A21_r2_combined GATATGCG_518_WM12A21_r2_combined TTTGGCCA_1385_WM12A21_r2_combined GGTTTCTG_668_WM12A21_r2_combined GCTGAAGA_706_WM12A21_r2_combined CCACTACA_32_WM12A21_r2_combined CTGTGCGG_1153_WM12A21_r2_combined CGTCTGTG_809_WM12A21_r2_combined TTTGACAT_1497_WM12A21_r2_combined GAGTTATA_1357_WM12A21_r2_combined TCTGAGTA_1672_WM12A21_r2_combined TTTTAGTG_1316_WM12A21_r2_combined TTCATAGG_1223_WM12A21_r2_combined CCTCCATA_1229_WM12A21_r2_combined ATTATCTG_55_WM12A21_r2_combined GGGAGAAC_1182_WM12A21_r2_combined AGTTGGAG_1602_WM12A21_r2_combined CCCGCTGA_1676_WM12A21_r2_combined CTTAATAC_33_WM12A21_r2_combined GTGAACGC_848_WM12A21_r2_combined GCGTCGCG_135_WM12A21_r2_combined ATTGGCCT_1333_WM12A21_r2_combined TGTGAGTG_1283_WM12A21_r2_combined CTAAAGGC_289_WM12A21_r2_combined AATATTAG_584_WM12A21_r2_combined CGGGGTAG_42_WM12A21_r2_combined ACCTATTT_1632_WM12A21_r2_combined AATTGGGG_269_WM12A21_r2_combined TAGCTTCG_1712_WM12A21_r2_combined TTATACTG_717_WM12A21_r2_combined CCAAAGGG_232_WM12A21_r2_combined GCAACGCC_1865_WM12A21_r2_combined CATAACGC_1613_WM12A21_r2_combined GCGACCTG_1295_WM12A21_r2_combined TATGCCTG_132_WM12A21_r2_combined ACTACTTA_1646_WM12A21_r2_combined GAGCTGAA_1600_WM12A21_r2_combined CGTAGTTC_722_WM12A21_r2_combined GGTCCTCA_1433_WM12A21_r2_combined ATCTTCCT_37_WM12A21_r2_combined CTTTCGAA_231_WM12A21_r2_combined CGTTAAGG_182_WM12A21_r2_combined CTCCTATA_1689_WM12A21_r2_combined AAACTTCA_1201_WM12A21_r2_combined CGCGTTAG_1690_WM12A21_r2_combined GAAAAGCT_1296_WM12A21_r2_combined CTCTTCAG_75_WM12A21_r2_combinedCGAGGTGG_31_WM12A21_r2_combined GCTTAGGA_89_WM12A21_r2_combined CCACGGGC_689_WM12A21_r2_combined GTTCTTTG_36_WM12A21_r2_combined AATATGAA_57_WM12A21_r2_combined GGAGGCTA_1567_WM12A21_r2_combined CAGCTCCC_1556_WM12A21_r2_combined TTCAACGG_136_WM12A21_r2_combined CATGTCAA_1533_WM12A21_r2_combined ATCGCCGT_1361_WM12A21_r2_combined CGCAGTAG_1428_WM12A21_r2_combinedGTGTATTG_1643_WM12A21_r2_combined GCGGGTAT_1278_WM12A21_r2_combined ATTTAAAT_1515_WM12A21_r2_combined TGCATACG_61_WM12A21_r2_combined AACGCTCA_1517_WM12A21_r2_combined CTATGCTT_1553_WM12A21_r2_combined TTACTACA_1106_WM12A21_r2_combined ACTGGCGC_1354_WM12A21_r2_combined GCATGCCC_1118_WM12A21_r2_combined ATGTTGGT_1158_WM12A21_r2_combined GTCACGGT_927_WM12A21_r2_combined TTACTCTG_30_WM12A21_r2_combined CATGTGTA_803_WM12A21_r2_combined CTTAGCTA_1307_WM12A21_r2_combined TATGCATC_1373_WM12A21_r2_combined TAATTGGT_1479_WM12A21_r2_combined CGAGGTAA_1205_WM12A21_r2_combined GAGCTGAC_71_WM12A21_r2_combined GAATCTAG_1650_WM12A21_r2_combined GAGAGTGG_653_WM12A21_r2_combined GCCGCAAG_724_WM12A21_r2_combined AACTGTAT_1319_WM12A21_r2_combined GTTTTGTA_1368_WM12A21_r2_combined ACTTCTGC_1334_WM12A21_r2_combined GTCCATAT_484_WM12A21_r2_combined CTAAGTCT_39_WM12A21_r2_combined ATGAAAAG_1660_WM12A21_r2_combined GTAGATTT_57_WM12A21_r2_combined AAAATGAG_665_WM12A21_r2_combined GCTGGCGT_1788_WM12A21_r2_combined GACACGAC_958_WM12A21_r2_combined CCCCGACA_1675_WM12A21_r2_combined GCGGCATG_1442_WM12A21_r2_combined TGTGCCAA_1508_WM12A21_r2_combined TCTATCTT_1494_WM12A21_r2_combined CCCGGAAG_1686_WM12A21_r2_combined CGATGAAT_52_WM12A21_r2_combined AGATTGCC_1744_WM12A21_r2_combined GTTGAGTT_1795_WM12A21_r2_combined TTTAACAG_757_WM12A21_r2_combined GAGAGCGT_1551_WM12A21_r2_combined TCCTCGGA_234_WM12A21_r2_combined ATCGCATC_1664_WM12A21_r2_combined CAGGAAGC_1196_WM12A21_r2_combined GGAACAAG_1471_WM12A21_r2_combined CTCTCATG_201_WM12A21_r2_combined TGGGCTGC_1158_WM12A21_r2_combined AGCAACGA_1468_WM12A21_r2_combined CTGCCTCT_1173_WM12A21_r2_combined GATTTGAT_63_WM12A21_r2_combined TCAGGTGC_1429_WM12A21_r2_combined CCAATTTG_824_WM12A21_r2_combined TAGGTGTT_699_WM12A21_r2_combined CCCATAAT_1272_WM12A21_r2_combined GCCCATTT_123_WM12A21_r2_combined AATCCGAG_1481_WM12A21_r2_combined TTAAGCTT_1318_WM12A21_r2_combined CCGTAAAC_1660_WM12A21_r2_combinedGGTCGGAG_1571_WM12A21_r2_combined GTAGTTGA_99_WM12A21_r2_combined CAGAGAAA_825_WM12A21_r2_combined GACAAGCC_199_WM12A21_r2_combined AGCTTGCT_1604_WM12A21_r2_combined TTTGTTTC_444_WM12A21_r2_combined ACCGGATT_1306_WM12A21_r2_combined TGCCTAAC_1419_WM12A21_r2_combined TGAGAACA_1649_WM12A21_r2_combinedAGTTCCGA_295_WM12A21_r2_combined GGTCCTTG_1564_WM12A21_r2_combined CTGTCGCA_34_WM12A21_r2_combined GTCAAAAC_1204_WM12A21_r2_combined CGTGCGAC_1207_WM12A21_r2_combined ATAGGGGC_1626_WM12A21_r2_combined GAGCAACT_1582_WM12A21_r2_combined ATTTCATT_1674_WM12A21_r2_combined ATAGTGAT_1453_WM12A21_r2_combined CGTTTCCT_1370_WM12A21_r2_combined ATTTGTAC_1552_WM12A21_r2_combined ATAGCGGG_1513_WM12A21_r2_combined GGGTGAGG_443_WM12A21_r2_combined GATATCAT_67_WM12A21_r2_combined ACGACTGT_1396_WM12A21_r2_combined AAACATAA_624_WM12A21_r2_combined CTGGGGAT_95_WM12A21_r2_combined GCATTCCA_1501_WM12A21_r2_combined TGATGGAC_1717_WM12A21_r2_combined GTTGCGCG_1692_WM12A21_r2_combinedGAAAACTA_322_WM12A21_r2_combined TGTTTACA_934_WM12A21_r2_combined ATAAGGCG_1191_WM12A21_r2_combined TATTGCTT_1623_WM12A21_r2_combined CCATGAAC_1622_WM12A21_r2_combined TACGTGTA_1569_WM12A21_r2_combined TCAGTGCG_1421_WM12A21_r2_combined AGTTCGCA_144_WM12A21_r2_combined GATATAGA_37_WM12A21_r2_combined GCCGAGTC_1342_WM12A21_r2_combined GGCTCACT_1764_WM12A21_r2_combined CCCATTTC_1176_WM12A21_r2_combined TTTGAATG_37_WM12A21_r2_combined TGCGGACT_712_WM12A21_r2_combined TGACGAGG_755_WM12A21_r2_combined CTTAAGTA_886_WM12A21_r2_combined GACAGTGA_1647_WM12A21_r2_combined CGCGAGAT_1639_WM12A21_r2_combined GTGCCAAA_9_WM12A21_r2_combined ACGCTGCC_1135_WM12A21_r2_combined TCCTATGC_1132_WM12A21_r2_combined ATAACGCC_49_WM12A21_r2_combined GATTAACG_39_WM12A21_r2_combined TATGCACG_1182_WM12A21_r2_combined TTCCATTG_217_WM12A21_r2_combined CGAATATA_207_WM12A21_r2_combined GATACGAG_1400_WM12A21_r2_combined ACAGAGAT_34_WM12A21_r2_combined CATTTTCA_1830_WM12A21_r2_combined CGCGTGCG_125_WM12A21_r2_combined CGATATCA_1466_WM12A21_r2_combined GGAGCGTG_1684_WM12A21_r2_combined CGGGCCAG_1311_WM12A21_r2_combined CGAGCATG_1598_WM12A21_r2_combinedACCTGTGT_1638_WM12A21_r2_combined GCTCCCTG_32_WM12A21_r2_combined ACGAGTTC_1372_WM12A21_r2_combined TATTGGAG_1536_WM12A21_r2_combined TGCTTGAA_601_WM12A21_r2_combined GTAAGGTC_1792_WM12A21_r2_combined GGAAGCTT_51_WM12A21_r2_combined AAACGCGG_65_WM12A21_r2_combined CTGGCAGT_741_WM12A21_r2_combined CACAGATA_1589_WM12A21_r2_combined GTGTTCAC_1213_WM12A21_r2_combined CAAGAAAA_43_WM12A21_r2_combined GGCGTCGT_115_WM12A21_r2_combined ACTGGCTT_1696_WM12A21_r2_combined GGATAGAG_747_WM12A21_r2_combined CGATCGTC_219_WM12A21_r2_combined TCCGCCTC_764_WM12A21_r2_combined GGTGGGCT_48_WM12A21_r2_combined CAAAACTT_127_WM12A21_r2_combined TTATGAAA_1549_WM12A21_r2_combined GGTTTAGC_1468_WM12A21_r2_combined CCTAGAAG_1139_WM12A21_r2_combined CTAGCAAA_804_WM12A21_r2_combined AAGCCATT_1618_WM12A21_r2_combined ATTTAGCC_1476_WM12A21_r2_combined TAAAATAC_1429_WM12A21_r2_combined GGCAATAG_69_WM12A21_r2_combinedTATCAGGC_1132_WM12A21_r2_combined TAGAGGCG_41_WM12A21_r2_combined TAACCGAA_724_WM12A21_r2_combined GGCTATGT_1413_WM12A21_r2_combined AAAGCTCC_1403_WM12A21_r2_combined TAACGCTT_1305_WM12A21_r2_combined TTGAAGGA_50_WM12A21_r2_combined GTACAAAA_37_WM12A21_r2_combined GAGGCTTT_1490_WM12A21_r2_combined TTCTGGCC_1313_WM12A21_r2_combined ACGCTACT_1280_WM12A21_r2_combined GATTCCGT_1329_WM12A21_r2_combined GTAGACTC_897_WM12A21_r2_combined CTTCACAG_885_WM12A21_r2_combined GTTATGCT_1590_WM12A21_r2_combined TTAAGTGC_261_WM12A21_r2_combined ATACTCCT_1545_WM12A21_r2_combined TTCCAACC_1374_WM12A21_r2_combined TAGAGGCA_1324_WM12A21_r2_combined TCGTTTGA_1243_WM12A21_r2_combined ATTATTTC_1236_WM12A21_r2_combined AAGCATCT_1700_WM12A21_r2_combined TCATCCAC_1765_WM12A21_r2_combined TATTGCCA_1800_WM12A21_r2_combined TATTGAAG_709_WM12A21_r2_combined GAGTAATC_1171_WM12A21_r2_combined TCAGTTAG_1404_WM12A21_r2_combined ACGATGTA_1660_WM12A21_r2_combined GGCCACCC_839_WM12A21_r2_combined AAGATTCG_185_WM12A21_r2_combined GAAATAGT_665_WM12A21_r2_combined GCGCAGAT_1625_WM12A21_r2_combined GATTCTGT_1377_WM12A21_r2_combined GAGGTCAT_198_WM12A21_r2_combined CCTATAGG_1714_WM12A21_r2_combined ATCATGAG_1493_WM12A21_r2_combined GTATGTTC_866_WM12A21_r2_combined CTTGCTGT_39_WM12A21_r2_combined AAAACATC_351_WM12A21_r2_combined GATTTTTA_1344_WM12A21_r2_combined GGTTGGCA_1597_WM12A21_r2_combined TCTGATTT_1354_WM12A21_r2_combined AAAGCTAA_1458_WM12A21_r2_combined GGTTTCCC_108_WM12A21_r2_combined ACACATGC_683_WM12A21_r2_combined TGGTGACT_1320_WM12A21_r2_combined TAGTGCAG_1447_WM12A21_r2_combined TTGGCAAG_919_WM12A21_r2_combined ACCACGAA_1363_WM12A21_r2_combined AGACCGTA_218_WM12A21_r2_combined TTTTCCGG_532_WM12A21_r2_combined TGGTTTGA_1308_WM12A21_r2_combined TAGCACTT_1600_WM12A21_r2_combined TTCTTTGC_1590_WM12A21_r2_combined CACGAACA_1321_WM12A21_r2_combined CACTTCAT_1546_WM12A21_r2_combined TGGACTTT_38_WM12A21_r2_combined TGAGGTCG_45_WM12A21_r2_combined TAGGGTGT_1553_WM12A21_r2_combined ATGTGATC_1791_WM12A21_r2_combined CCGCATAC_1872_WM12A21_r2_combined TTCAGGTG_59_WM12A21_r2_combined GTCACTGT_1525_WM12A21_r2_combined ACTCACCA_1674_WM12A21_r2_combined TACATCTA_829_WM12A21_r2_combined CGCTTCTC_1694_WM12A21_r2_combined CGCAACAT_1313_WM12A21_r2_combined GGCTTTAA_1628_WM12A21_r2_combined AGGTTATT_1604_WM12A21_r2_combined TGGAATGC_30_WM12A21_r2_combined TCATTAAG_1572_WM12A21_r2_combined TTCCTATC_111_WM12A21_r2_combined CAAACAGA_33_WM12A21_r2_combined CCGGAGAC_76_WM12A21_r2_combined CTACAGGA_1173_WM12A21_r2_combined AAAGAGTG_1219_WM12A21_r2_combined TTTAGGGC_197_WM12A21_r2_combined ATCATCTA_1303_WM12A21_r2_combined ATGACGCA_34_WM12A21_r2_combined ACGATCAG_62_WM12A21_r2_combined GCGGGTAA_132_WM12A21_r2_combined TCTTAGAA_1770_WM12A21_r2_combined TTTCCTAA_256_WM12A21_r2_combined CACACCCG_1279_WM12A21_r2_combined ATAACGGT_1666_WM12A21_r2_combined AATAACCG_743_WM12A21_r2_combined ACCATCTT_1697_WM12A21_r2_combined GTCCGTAC_1090_WM12A21_r2_combined TTTTTGTC_819_WM12A21_r2_combined CGGCAAAG_622_WM12A21_r2_combined ATGTAATA_1824_WM12A21_r2_combined TTGCACTT_72_WM12A21_r2_combined CGTCTTCC_1560_WM12A21_r2_combined CCCGCTCC_1523_WM12A21_r2_combined ACCTAAGC_59_WM12A21_r2_combined TTCATGCT_110_WM12A21_r2_combined GGTTGCGG_136_WM12A21_r2_combined CTTAATTC_101_WM12A21_r2_combined GCTGCAAA_890_WM12A21_r2_combined AACTCTGA_1469_WM12A21_r2_combined CCCCAAAT_1688_WM12A21_r2_combined TCAAGGGC_1208_WM12A21_r2_combined ACCCCCGT_38_WM12A21_r2_combined ACTAAAGA_1750_WM12A21_r2_combined TTTGGGTC_1040_WM12A21_r2_combined TTTACTGG_1640_WM12A21_r2_combined TGTGGGAC_840_WM12A21_r2_combined GCCTTGCC_1151_WM12A21_r2_combined TATCACGT_93_WM12A21_r2_combined CACATATG_1581_WM12A21_r2_combined AGTCTCGG_424_WM12A21_r2_combined CAAAACCT_769_WM12A21_r2_combined CGGACTCG_1435_WM12A21_r2_combined AGTACTGT_1619_WM12A21_r2_combined GCCAAGTA_151_WM12A21_r2_combined AAGAGTTT_1073_WM12A21_r2_combined TTATTGCG_619_WM12A21_r2_combined GTCTACAC_1490_WM12A21_r2_combined CTATCATT_412_WM12A21_r2_combined ACGCTGTT_1380_WM12A21_r2_combined GGCCAATC_112_WM12A21_r2_combined TTCCCGGG_1702_WM12A21_r2_combined GGCACCGA_239_WM12A21_r2_combined CGAAGTAC_67_WM12A21_r2_combined AGCGGCAA_1620_WM12A21_r2_combined GTCATTTT_1495_WM12A21_r2_combined GTTTTTTG_1385_WM12A21_r2_combined TATTATGG_1088_WM12A21_r2_combined CCCTTTAA_1166_WM12A21_r2_combined ACGATAAA_126_WM12A21_r2_combined CTCCCGCC_34_WM12A21_r2_combined TCTGCGTA_92_WM12A21_r2_combined GGCCTGTG_1304_WM12A21_r2_combined TTTTCACG_1668_WM12A21_r2_combined GCAATACG_1726_WM12A21_r2_combined AAAGTCCG_440_WM12A21_r2_combined GGAAAGAA_828_WM12A21_r2_combined ATCGTGCG_1335_WM12A21_r2_combined AGTGGATA_67_WM12A21_r2_combined AACCCTTT_2250_WM12A21_r2_combined CTATATGA_131_WM12A21_r2_combined GCTAATGC_1333_WM12A21_r2_combined GGTTTTAA_5_WM12A21_r2_combined AGGTCTAT_176_WM12A21_r2_combined GGCAAGTA_490_WM12A21_r2_combined GCTGCTTT_780_WM12A21_r2_combined TGAACTAG_7_WM12A21_r2_combined GAAACAGG_2_WM12A21_r2_combined GATCCCAC_1163_WM12A21_r2_combined AGATTGGA_586_WM12A21_r2_combined TGCTCTTG_1615_WM12A21_r2_combined TTTACACT_252_WM12A21_r2_combined CTACTGGA_1379_WM12A21_r2_combined TGGGCCTT_1507_WM12A21_r2_combined CGGCTTCT_1333_WM12A21_r2_combined GAGTAGAT_1083_WM12A21_r2_combined CTGGACTC_1071_WM12A21_r2_combined GTTTGCGA_545_WM12A21_r2_combined GCTTACAG_1585_WM12A21_r2_combined TGTCTGCG_1591_WM12A21_r2_combined CGGAGTGT_1196_WM12A21_r2_combined TAACCATG_1288_WM12A21_r2_combined ACGTCGTC_56_WM12A21_r2_combined CAAGCCTT_132_WM12A21_r2_combined GCTTGTTA_34_WM12A21_r2_combined ACATTGCT_356_WM12A21_r2_combined CTTAAATT_88_WM12A21_r2_combined ACTTACGG_59_WM12A21_r2_combined CGCCCTGT_409_WM12A21_r2_combined AGTAGGCA_922_WM12A21_r2_combined AGTTCTTA_134_WM12A21_r2_combined CTCCAAAG_1740_WM12A21_r2_combined GCTTGTCT_1709_WM12A21_r2_combined TCGTGGGC_34_WM12A21_r2_combined CCATCTTA_1347_WM12A21_r2_combined ATATGCAT_1260_WM12A21_r2_combined CTATCATC_399_WM12A21_r2_combined CTGGGCGT_1393_WM12A21_r2_combined CCTAATGC_986_WM12A21_r2_combined TGAGGGGT_103_WM12A21_r2_combined GCTAGCCT_1325_WM12A21_r2_combined TGGTGCTG_1535_WM12A21_r2_combined TTCGCTCT_1769_WM12A21_r2_combined ACATGACT_1697_WM12A21_r2_combined CCTCTTTG_1258_WM12A21_r2_combined GCGGATAT_1772_WM12A21_r2_combined ATAGACAG_34_WM12A21_r2_combined CACAGCGG_131_WM12A21_r2_combined TTGCTCCC_1485_WM12A21_r2_combined CTAAGGGC_1490_WM12A21_r2_combined TCCAATTG_1512_WM12A21_r2_combined GGTGGTTT_1693_WM12A21_r2_combined AAAGCATA_159_WM12A21_r2_combined ACTAGTGC_1757_WM12A21_r2_combined CTAGCCCA_1332_WM12A21_r2_combined CAAAGTGT_34_WM12A21_r2_combined GGTCCCTG_825_WM12A21_r2_combined GCCGAGGC_1665_WM12A21_r2_combined GGGGGACG_39_WM12A21_r2_combinedCAAGAAGT_45_WM12A21_r2_combined GGCAATCT_1012_WM12A21_r2_combined GCTTGCGG_1690_WM12A21_r2_combined AAGGGCAA_657_WM12A21_r2_combined TAGCACGT_1521_WM12A21_r2_combined CAGACCTG_1221_WM12A21_r2_combined GCACCCTG_31_WM12A21_r2_combined TTCTGCTT_1180_WM12A21_r2_combined ACTACTTG_86_WM12A21_r2_combined CGGCGTGA_716_WM12A21_r2_combined TGTTACTT_465_WM12A21_r2_combined AAGGCGGA_1528_WM12A21_r2_combined TATCTATG_1086_WM12A21_r2_combined ATGCAGTC_944_WM12A21_r2_combined ATACGCTG_1531_WM12A21_r2_combined CTTCCTGC_1190_WM12A21_r2_combined CAGCAATG_144_WM12A21_r2_combined ATGTGCAT_1484_WM12A21_r2_combined TTAGGTTT_1411_WM12A21_r2_combined CAATACTT_1568_WM12A21_r2_combined CTCGTTAT_36_WM12A21_r2_combined TTTATCTA_1552_WM12A21_r2_combined TCCTGAAG_118_WM12A21_r2_combined AGCAACAT_1169_WM12A21_r2_combined CTCTTGGA_1753_WM12A21_r2_combined CCTCAGTA_350_WM12A21_r2_combined TTTCTTTA_31_WM12A21_r2_combined GGGGAATA_326_WM12A21_r2_combined TCGCGTGA_369_WM12A21_r2_combined GCAATAAA_1296_WM12A21_r2_combined GGTGGTAG_39_WM12A21_r2_combined CCAAGAAA_601_WM12A21_r2_combined GATTAGGT_1301_WM12A21_r2_combined ATTGGGCT_70_WM12A21_r2_combined GCCCAAAG_32_WM12A21_r2_combined CATCATTG_1710_WM12A21_r2_combined TACGAAAG_1631_WM12A21_r2_combined ACTAGCTA_1471_WM12A21_r2_combined TATGAGGT_40_WM12A21_r2_combined AATAGGTT_1977_WM12A21_r2_combined CTGTGCCT_1659_WM12A21_r2_combined GGGTCATA_49_WM12A21_r2_combined CAGGCGTA_187_WM12A21_r2_combined GGGTCAAT_1195_WM12A21_r2_combined ACATCATG_1300_WM12A21_r2_combined ACAAGGTA_198_WM12A21_r2_combined AGCCTCTA_156_WM12A21_r2_combined AAGTACAA_60_WM12A21_r2_combined TCCCGCGT_1539_WM12A21_r2_combined GTGTCCTG_1473_WM12A21_r2_combined CAATGCAT_1666_WM12A21_r2_combined TAGGATTT_1284_WM12A21_r2_combined GGTATGCG_73_WM12A21_r2_combined TAGGGCCT_1321_WM12A21_r2_combined CCGCTTCT_1798_WM12A21_r2_combined CGTCTAGA_1380_WM12A21_r2_combined CGGCCCCC_1442_WM12A21_r2_combined AGGTTCAG_72_WM12A21_r2_combined ACTTATGT_1292_WM12A21_r2_combined CTTATATA_743_WM12A21_r2_combined TGCCATAC_37_WM12A21_r2_combined TCGTTACC_241_WM12A21_r2_combined GTTCCGTT_1416_WM12A21_r2_combined AATTGTCG_132_WM12A21_r2_combined GTCGTTTG_1502_WM12A21_r2_combined TTTATTTT_849_WM12A21_r2_combined CGTCATCG_1501_WM12A21_r2_combined TCGAGTAA_1542_WM12A21_r2_combined CAGCTGGG_1359_WM12A21_r2_combined ATCATTTT WM12A21_r2_combined GTCATAGG_58_WM12A21_r2_combined TTTAATCT_1377_WM12A21_r2_combined TAGCGCAG_1538_WM12A21_r2_combined AATCCGAA_1582_WM12A21_r2_combined CACGGTGT_76_WM12A21_r2_combined ACCTTGCC_1300_WM12A21_r2_combined GTAGGTAG_106_WM12A21_r2_combined AATGAATG_1741_WM12A21_r2_combinedTGCACGAC_1654_WM12A21_r2_combined CACGAAAA_233_WM12A21_r2_combined GGTAGACT_837_WM12A21_r2_combined GGTTACCC_1583_WM12A21_r2_combined CGTTCCCG_1658_WM12A21_r2_combined CGAAACCT_1652_WM12A21_r2_combined CAGGTCGT_1650_WM12A21_r2_combined CCTTAGGC_1474_WM12A21_r2_combinedCGTGAGCA_62_WM12A21_r2_combined TAGGGCCG_35_WM12A21_r2_combined AATCAGTT_1544_WM12A21_r2_combined GCTTAGGT_1037_WM12A21_r2_combined GGGTCTTC_148_WM12A21_r2_combined TTTGGTCC_1287_WM12A21_r2_combined CCTTTTGC_1273_WM12A21_r2_combined CCAGAAGC_75_WM12A21_r2_combined GTACAAAG_30_WM12A21_r2_combined TTGTCTGT_1674_WM12A21_r2_combined ATGTCTCC_59_WM12A21_r2_combined GGGAGACG_1191_WM12A21_r2_combined CCGAGGTT_401_WM12A21_r2_combined CCAGAAAC_1475_WM12A21_r2_combined TAGCCGAT_1403_WM12A21_r2_combined ACAACACT_1816_WM12A21_r2_combined GTACTGAG_144_WM12A21_r2_combined TTGTTAGG_1532_WM12A21_r2_combined CTTCCTAA_1626_WM12A21_r2_combined GAGCATCA_61_WM12A21_r2_combined TGCTACCC_1229_WM12A21_r2_combined TTCGGGTA_1177_WM12A21_r2_combined ATCTTCTT_1626_WM12A21_r2_combined CAGGGAGA_466_WM12A21_r2_combined CGGCCGTG_91_WM12A21_r2_combined GGAACTTC_524_WM12A21_r2_combined ACTCCAAT_92_WM12A21_r2_combined AGGGAATT_61_WM12A21_r2_combined GAATTGTG_1081_WM12A21_r2_combinedTAATCTGA_707_WM12A21_r2_combined GGATTGAT_34_WM12A21_r2_combined CTACACGT_1706_WM12A21_r2_combined CCCGGGTC_1532_WM12A21_r2_combined ATACGAGA_359_WM12A21_r2_combined AGTGCGCG_37_WM12A21_r2_combined CTGACCCA_51_WM12A21_r2_combined GAGCAGGA_1506_WM12A21_r2_combined AAGAAGGT_1207_WM12A21_r2_combined CGCATGGT_270_WM12A21_r2_combined CGATGCCT_118_WM12A21_r2_combined GCACTTCA_30_WM12A21_r2_combined CGTATGTG_953_WM12A21_r2_combined CATGGACT_151_WM12A21_r2_combined CATAGCGA_1333_WM12A21_r2_combined GCGGGGAA_1522_WM12A21_r2_combined CTCATGAA_1660_WM12A21_r2_combined CGCATGCC_104_WM12A21_r2_combined CTGCCAGC_1544_WM12A21_r2_combinedTCGGTGCT_53_WM12A21_r2_combined TCAGGGAT_131_WM12A21_r2_combined ATATGCTC_1474_WM12A21_r2_combined ATACTCAG_1086_WM12A21_r2_combined GACACTGG_1581_WM12A21_r2_combined ATCATTCG_1720_WM12A21_r2_combined AATTTCCC_652_WM12A21_r2_combined CTATAAGT_1232_WM12A21_r2_combined AGAGTTAC_157_WM12A21_r2_combined GAATCCAG_1829_WM12A21_r2_combined GTTCGGAT_1560_WM12A21_r2_combined CGTGCCTT_1575_WM12A21_r2_combined CACGCTGG_1208_WM12A21_r2_combined GGGATTTC_1147_WM12A21_r2_combined GGTATGAT_833_WM12A21_r2_combined CGGTTAAA_1070_WM12A21_r2_combined AATAGGGT_227_WM12A21_r2_combined CAGGCTGC_1544_WM12A21_r2_combined CGGTCTCT_1374_WM12A21_r2_combined CGCCTAGG_1763_WM12A21_r2_combined AGGTCGTT_876_WM12A21_r2_combined ACAGGGCC_1738_WM12A21_r2_combined GTTTTAGT_33_WM12A21_r2_combined GGACCTTG_815_WM12A21_r2_combinedGTATGGGA_670_WM12A21_r2_combined AGCCAGGT_1817_WM12A21_r2_combined CTTGGTGG_899_WM12A21_r2_combined GCCGTATT_1680_WM12A21_r2_combined GCGCAGTT_1117_WM12A21_r2_combined ATAAAATG_1154_WM12A21_r2_combined TGCTGCTC_1501_WM12A21_r2_combined TTCGATAA_128_WM12A21_r2_combined TGGATCAG_120_WM12A21_r2_combined GTACCCTT_1335_WM12A21_r2_combined CCAGACGC_235_WM12A21_r2_combined GCCGCCAA_1387_WM12A21_r2_combined GTAAGCTT_1186_WM12A21_r2_combined GCGACGAC_772_WM12A21_r2_combined TGTCTAAC_934_WM12A21_r2_combined AACACACA_1305_WM12A21_r2_combined TCTTGTGG_1602_WM12A21_r2_combined TCGGGTCC_1617_WM12A21_r2_combined TGGCCCAA_997_WM12A21_r2_combined AGAGCGTT_1623_WM12A21_r2_combined GGCGGTTT_32_WM12A21_r2_combined GAACCGTC_1082_WM12A21_r2_combined ACCCTCTT_1741_WM12A21_r2_combined GCCCTACT_1361_WM12A21_r2_combined GGGTATGG_1268_WM12A21_r2_combined GGTATACC_1500_WM12A21_r2_combined TAAGCCTT_1569_WM12A21_r2_combined TCTCGGTC_1374_WM12A21_r2_combined GCCTGGTG_1382_WM12A21_r2_combined TCGTTGGA_1483_WM12A21_r2_combined AAAAAGGA_1174_WM12A21_r2_combined TTTTACCT_1342_WM12A21_r2_combined GTAGCTTC_737_WM12A21_r2_combined CGTAACTT_1288_WM12A21_r2_combined GTCTTCAG_1193_WM12A21_r2_combined ACGTAGCA_1491_WM12A21_r2_combined TGTACCTA_1628_WM12A21_r2_combined CATTTAAC_738_WM12A21_r2_combined TGGCTTAC_87_WM12A21_r2_combined TCCGGTAG_68_WM12A21_r2_combined GTTCATCA_43_WM12A21_r2_combined CCGGCAGT_829_WM12A21_r2_combined ATTATCTC_1253_WM12A21_r2_combined TGAATTGC_315_WM12A21_r2_combined TGATCGAG_138_WM12A21_r2_combined CGAACGGC_120_WM12A21_r2_combined GTTTACAG_1405_WM12A21_r2_combined CAATTTAA_125_WM12A21_r2_combined CTTAAACA_145_WM12A21_r2_combined TACAAAGA_792_WM12A21_r2_combined CGCATCTG_1231_WM12A21_r2_combined CAGGCTTC_812_WM12A21_r2_combined GTCTTGTT_1209_WM12A21_r2_combined CGATGTAT_1410_WM12A21_r2_combined ACAATCGG_1875_WM12A21_r2_combined TCCTGGTA_31_WM12A21_r2_combined TAAAGGCT_62_WM12A21_r2_combined TCTCGCCT_212_WM12A21_r2_combined TTAGCTTG_32_WM12A21_r2_combined GGGGATCT_1314_WM12A21_r2_combined 0.02 CATTCTGA_3759_WM12A19_r2_combined TGGGGTTG_7361_WM12A19_r2_combined ATATCACG_149_WM12A19_r2_combined CTTATGCT_137_WM12A19_r2_combined AGGGGCCT_595_WM12A19_r2_combined TATTCCCT_113_WM12A19_r2_combined CGGTAGAG_147_WM12A19_r2_combined CCGATGGC_3905_WM12A19_r2_combined TAGATATC_955_WM12A19_r2_combined AGATAAAA_262_WM12A19_r2_combined GTAGTTGT_9171_WM12A19_r2_combined GTAAGGAG_9467_WM12A19_r2_combined TCAAAAGC_112_WM12A19_r2_combined ACCCTTTT_88_WM12A19_r2_combined AGTAAACT_8093_WM12A19_r2_combined TCCTTGGT_7613_WM12A19_r2_combined TGGAACCC_462_WM12A19_r2_combined AGCGCCAA_212_WM12A19_r2_combined CTCTCCTC_7567_WM12A19_r2_combined CCGTGCCG_144_WM12A19_r2_combined ATGTCATT_177_WM12A19_r2_combined TGAGGTTC_4601_WM12A19_r2_combined TCTTCACC_185_WM12A19_r2_combined CTGTCCTA_1951_WM12A19_r2_combined TTCGGTTT_289_WM12A19_r2_combined GATATTTC_7056_WM12A19_r2_combined TTCGCTCC_166_WM12A19_r2_combined TTATCTTA_1246_WM12A19_r2_combined GTAGTTTT_535_WM12A19_r2_combined GGTCCTGT_195_WM12A19_r2_combined TTTTCGGC_120_WM12A19_r2_combined ATCACTGA_7757_WM12A19_r2_combined CGCCTTTT_90_WM12A19_r2_combined AGACGCCA_8807_WM12A19_r2_combined AAACTTCG_302_WM12A19_r2_combined CTACACCA_305_WM12A19_r2_combined GCGTCGGT_8044_WM12A19_r2_combined CAGTAAGT_7280_WM12A19_r2_combined CCAATAGA_9659_WM12A19_r2_combined CCCCACTC_222_WM12A19_r2_combined TACGGAGT_119_WM12A19_r2_combined CTATGAGA_230_WM12A19_r2_combined AGCGCGGT_9417_WM12A19_r2_combined AGGATGTG_814_WM12A19_r2_combined CTGGGCCA_7667_WM12A19_r2_combined ACAACCTT_2382_WM12A19_r2_combined TATATAAT_91_WM12A19_r2_combined CCTCCAAC_102_WM12A19_r2_combined GGTTCGTA_371_WM12A19_r2_combined TAGAGGGT_1110_WM12A19_r2_combined TTGCCAAT_195_WM12A19_r2_combined GGATTGTA_8506_WM12A19_r2_combined TTATCAAC_1029_WM12A19_r2_combined TGTGGCAC_8908_WM12A19_r2_combined ATTGTCCC_92_WM12A19_r2_combined AACGACCT_169_WM12A19_r2_combined AGGGATCT_182_WM12A19_r2_combined ACGCATTT_782_WM12A19_r2_combined 0.02 TTGCGGAA_876_WM12A18_r2_combined TCCGTTGG_1338_WM12A18_r2_combined TAACGCCC_1406_WM12A18_r2_combined TACTACGG_1423_WM12A18_r2_combined TAAGGATT_1436_WM12A18_r2_combined GCTAGTAG_39_WM12A18_r2_combined AGTTTGCT_1799_WM12A18_r2_combined GTTTTGAA_1258_WM12A18_r2_combined CATCGTAA_782_WM12A18_r2_combined ATTTCGTG_924_WM12A18_r2_combined ACTTCGGA_691_WM12A18_r2_combined GTAAGTCT_1026_WM12A18_r2_combined GATAGTCG_1343_WM12A18_r2_combined TAGTTCGG_220_WM12A18_r2_combined CAGAGGTA_814_WM12A18_r2_combined TCATTATC_1095_WM12A18_r2_combined GCAAAGCT_805_WM12A18_r2_combined CATGTTTA_442_WM12A18_r2_combined GCTTTGGG_256_WM12A18_r2_combined TCTGAGTT_356_WM12A18_r2_combined CGCTCCTT_487_WM12A18_r2_combined CTACACTT_1543_WM12A18_r2_combined AGAGCCCC_1060_WM12A18_r2_combined AGCTTTGT_743_WM12A18_r2_combined CACAAGTC_1208_WM12A18_r2_combined CCTTCCAT_1814_WM12A18_r2_combined ACAGCAGG_799_WM12A18_r2_combined CAACGAGC_42_WM12A18_r2_combined TGGTGACC_134_WM12A18_r2_combined ACGGTTCA_1197_WM12A18_r2_combined TGCAGGAC_1105_WM12A18_r2_combined TTTTACGC_1282_WM12A18_r2_combined ACTCTTAC_39_WM12A18_r2_combined TACGACTT_1128_WM12A18_r2_combined GGGGGTTT_788_WM12A18_r2_combined GATGTTGA_1782_WM12A18_r2_combined TTAAAAAC_1123_WM12A18_r2_combined AGTTGCGC_212_WM12A18_r2_combined AGGGCCAG_277_WM12A18_r2_combined AAAACTGC_841_WM12A18_r2_combined TTTGTGTG_41_WM12A18_r2_combined CCCCGTGC_1355_WM12A18_r2_combined ACTTCAAA_1092_WM12A18_r2_combined TTATCTCC_1102_WM12A18_r2_combined TTTTGCTG_536_WM12A18_r2_combined GGCAGATG_1791_WM12A18_r2_combined TGGCAAAC_610_WM12A18_r2_combined ATACATCG_789_WM12A18_r2_combined GCCAGGCT_1688_WM12A18_r2_combined GTTGGCCA_95_WM12A18_r2_combined CCAGAATA_76_WM12A18_r2_combined GAATTTAG_465_WM12A18_r2_combined GACATATT_804_WM12A18_r2_combined AGGAACCC_1039_WM12A18_r2_combined CTCAGAGG_1626_WM12A18_r2_combined GCAGGTGC_1084_WM12A18_r2_combined GGATCCGG_1656_WM12A18_r2_combined AACCAGGT_66_WM12A18_r2_combined GAGGCAAC_81_WM12A18_r2_combined TGTCGGTA_1315_WM12A18_r2_combined ATTGAGCG_1290_WM12A18_r2_combined GATGACTG_1266_WM12A18_r2_combined GAACTGAG_83_WM12A18_r2_combined ACTTGAAG_66_WM12A18_r2_combined TCTCTAGA_1246_WM12A18_r2_combined CAGTTTTG_311_WM12A18_r2_combined ATCTAGCC_1222_WM12A18_r2_combined TATATGCG_98_WM12A18_r2_combined GCTGAAGG_2058_WM12A18_r2_combined GGAAAGAT_1601_WM12A18_r2_combined GCCCGAGA_1510_WM12A18_r2_combined CTATGAAG_683_WM12A18_r2_combined AGAGCAGT_1312_WM12A18_r2_combined TCAGTAGT_125_WM12A18_r2_combined GCATGGCC_1523_WM12A18_r2_combined TTTCCTCG_110_WM12A18_r2_combined CTCGGCAT_531_WM12A18_r2_combined GGAAGATT_41_WM12A18_r2_combined AGATGGTA_245_WM12A18_r2_combined ACTCCTTT_638_WM12A18_r2_combined ATTATCGA_1396_WM12A18_r2_combined CTCGTAGG_912_WM12A18_r2_combined GACACTGT_1360_WM12A18_r2_combined TATCATAC_1499_WM12A18_r2_combined AGCATCCT_982_WM12A18_r2_combined AAAATACA_618_WM12A18_r2_combined CTTAAATT_1662_WM12A18_r2_combined CCAGGCCG_549_WM12A18_r2_combined GAGAGATA_1954_WM12A18_r2_combined CACTGGTA_1265_WM12A18_r2_combined CCATACCG_1110_WM12A18_r2_combined GCTATGGA_140_WM12A18_r2_combined CAGCTTTG_1408_WM12A18_r2_combined GCGCCAAC_646_WM12A18_r2_combined AAATCGTC_1427_WM12A18_r2_combined TAGGTAAA_1970_WM12A18_r2_combined TCTTAATT_1063_WM12A18_r2_combined CTAGAATA_887_WM12A18_r2_combined CCGTCTTA_809_WM12A18_r2_combined TGACTTTT_1202_WM12A18_r2_combined GATGGTCT_1309_WM12A18_r2_combined AGCCGCGG_928_WM12A18_r2_combined GAGGTGTA_1695_WM12A18_r2_combined TAAATTCG_1383_WM12A18_r2_combined TAAAGGCA_1680_WM12A18_r2_combined TCCCATGC_1274_WM12A18_r2_combined CATAACGG_511_WM12A18_r2_combined ATGGTGTG_1428_WM12A18_r2_combined CCTCTCTT_1438_WM12A18_r2_combined TAGGGCCA_897_WM12A18_r2_combined ATAACGGA_1474_WM12A18_r2_combined AAGATTCT_1216_WM12A18_r2_combined TCTACGAA_1629_WM12A18_r2_combined GTAACTTG_1404_WM12A18_r2_combined TTCACAAC_1233_WM12A18_r2_combined GGCCCTCC_638_WM12A18_r2_combined ATTTCTTA_64_WM12A18_r2_combined CACATCCA_1520_WM12A18_r2_combined CATTAATA_1787_WM12A18_r2_combined TCATGTTT_1140_WM12A18_r2_combined ATTTACAC_662_WM12A18_r2_combined CATCAACG_593_WM12A18_r2_combined TTGAATTG_43_WM12A18_r2_combined CGGACGAC_1347_WM12A18_r2_combined CACTCCAG_471_WM12A18_r2_combined CAGGTGGA_38_WM12A18_r2_combined TTGCAGTA_220_WM12A18_r2_combined CTACCCAC_196_WM12A18_r2_combined CGCCAGGC_1709_WM12A18_r2_combined TGGCTCGA_1239_WM12A18_r2_combined CATTCTTC_668_WM12A18_r2_combined ATCGTCCC_854_WM12A18_r2_combined TAGGGTGA_1442_WM12A18_r2_combined AGCTGCGC_773_WM12A18_r2_combined TCTTATCA_624_WM12A18_r2_combined CCTGCCTT_1216_WM12A18_r2_combined GATCAAAC_42_WM12A18_r2_combined AAGAGGGA_609_WM12A18_r2_combined TCACCTCT_221_WM12A18_r2_combined AACTCTAG_90_WM12A18_r2_combined CGCTACGT_52_WM12A18_r2_combined AGATTTAT_1147_WM12A18_r2_combined ATGCGGGG_1068_WM12A18_r2_combined CCGGGAAA_1127_WM12A18_r2_combined AAGACTTG_47_WM12A18_r2_combined AAATTTAC_1772_WM12A18_r2_combined AAGACTGA_60_WM12A18_r2_combined AGGGCTGA_1716_WM12A18_r2_combined TATTAGGG WM12A18_r2_combined TATATCTC_123_WM12A18_r2_combined GATCTCGT_203_WM12A18_r2_combined GGATACCA_90_WM12A18_r2_combined GGTTGTTT_1322_WM12A18_r2_combined AATTTGTT_1206_WM12A18_r2_combined CCCGATTT_101_WM12A18_r2_combined GGTTTGCT_97_WM12A18_r2_combined GGGTCTAT_1053_WM12A18_r2_combined AGGGGGTT_206_WM12A18_r2_combined GTGTCTAC_997_WM12A18_r2_combined AATTCAGG_1285_WM12A18_r2_combined CCCGCTAG_851_WM12A18_r2_combined AGGAAAAG_1657_WM12A18_r2_combined CCAGGGTA_1459_WM12A18_r2_combined GCTAATTG_759_WM12A18_r2_combined AAGTTGCT_1315_WM12A18_r2_combined ATTTTGAT_1217_WM12A18_r2_combined TTCGCTGA_1036_WM12A18_r2_combined CCTTGAGG_259_WM12A18_r2_combined GTTCCCCT_413_WM12A18_r2_combined GACATTAC_199_WM12A18_r2_combined AGACACGA_895_WM12A18_r2_combined AGGATCGC_669_WM12A18_r2_combined AGAAACAC_62_WM12A18_r2_combined CATGAACA_824_WM12A18_r2_combined GAGGCGGG_1022_WM12A18_r2_combined TCAGGGTG_1507_WM12A18_r2_combined GTCCATAG_303_WM12A18_r2_combined TCTTAACC_500_WM12A18_r2_combined TGATTTAC_1170_WM12A18_r2_combined AGCCTTCG_1521_WM12A18_r2_combined TGAACAAT_246_WM12A18_r2_combined TAGCCCCA_185_WM12A18_r2_combined CCCTTCGT_659_WM12A18_r2_combined ATAGCTCA_629_WM12A18_r2_combined CAATGGTC_248_WM12A18_r2_combined GGGCTTAA_409_WM12A18_r2_combined TCAACATC_1113_WM12A18_r2_combined TGCTGTAA_1316_WM12A18_r2_combined TTTATGTA_1573_WM12A18_r2_combined GATATCTC_1057_WM12A18_r2_combined TCCCCTTG_1755_WM12A18_r2_combined ACGTTTGT_1450_WM12A18_r2_combined GAACTCCT_719_WM12A18_r2_combined ATTTGCCC_2092_WM12A18_r2_combined GTAGTTGT_1080_WM12A18_r2_combined CGAGACGT_40_WM12A18_r2_combined GTTATTCT_828_WM12A18_r2_combined ACCAGGGA_1405_WM12A18_r2_combined ATTGTATA_1312_WM12A18_r2_combined CCGGCATT_561_WM12A18_r2_combined TGGTTGAT_129_WM12A18_r2_combined AGTTCGGA_2089_WM12A18_r2_combined TGAGTGGC_749_WM12A18_r2_combined TAGGATAG_41_WM12A18_r2_combined CAAGACGT_1580_WM12A18_r2_combined GAACCTTT_325_WM12A18_r2_combined TATTGACA_1564_WM12A18_r2_combined GTAAGTAG_989_WM12A18_r2_combined ATGACTTT_1380_WM12A18_r2_combined AAGCTCGC_128_WM12A18_r2_combined CGATGCAG_2049_WM12A18_r2_combined TCTAAAGT_47_WM12A18_r2_combined ATACCAAT_562_WM12A18_r2_combined ACATCTGC_1293_WM12A18_r2_combined ACTGGACA_55_WM12A18_r2_combined GTCATTTG_1805_WM12A18_r2_combined AATCAGAG_2148_WM12A18_r2_combined GATTTACA_1534_WM12A18_r2_combined TTCGGCAT_95_WM12A18_r2_combined TTAGATGG_2203_WM12A18_r2_combined CACGGCGA_733_WM12A18_r2_combined GGTTCGTA_1258_WM12A18_r2_combined CCGACGAA_1978_WM12A18_r2_combined GTTCTTTA_1036_WM12A18_r2_combined TGGGATGG_2096_WM12A18_r2_combined CAAGTGAT_939_WM12A18_r2_combined TCTAAATA_1139_WM12A18_r2_combined TAAGACTA_482_WM12A18_r2_combined TATAGTTA_624_WM12A18_r2_combined GCGCTTTG_1018_WM12A18_r2_combined GCAAACAC_63_WM12A18_r2_combined GCCCCAGT_1388_WM12A18_r2_combined TTTATGTT_602_WM12A18_r2_combined ACAATGAT_36_WM12A18_r2_combined GGAGGCGC_1483_WM12A18_r2_combined CTAAATCT_335_WM12A18_r2_combined GTCTCAGT_38_WM12A18_r2_combined CTAGCACA_144_WM12A18_r2_combined GACATCAT_160_WM12A18_r2_combined GCCCAGTC_709_WM12A18_r2_combined ATACCGCC_1258_WM12A18_r2_combined CTGGTAGG_1323_WM12A18_r2_combined TTGCCTAA_1672_WM12A18_r2_combined ATCTAGCT_1312_WM12A18_r2_combined TGAAAGGA_152_WM12A18_r2_combined TTAAGTAT_710_WM12A18_r2_combined GCGTTTGT_1081_WM12A18_r2_combined TGCCGTTA_1181_WM12A18_r2_combined GACCCACC_70_WM12A18_r2_combined GCCGAATT_69_WM12A18_r2_combined CCGTCTCA_1031_WM12A18_r2_combined GATAGGAG_1779_WM12A18_r2_combined CGCGTACA_1299_WM12A18_r2_combined TTTAGTCC_808_WM12A18_r2_combined AACTGCCG_1719_WM12A18_r2_combined TACCCGAG_1402_WM12A18_r2_combined TTTACTCG_279_WM12A18_r2_combined TTTGTTCT_73_WM12A18_r2_combined GCCTGAAT_74_WM12A18_r2_combined CTTCATCG_74_WM12A18_r2_combined TCGTTAGC_984_WM12A18_r2_combined TCGGACTA_1449_WM12A18_r2_combined TCTTTCTC_224_WM12A18_r2_combined TTCGCGAT_82_WM12A18_r2_combined GACTTGAA_1250_WM12A18_r2_combined TGTATTAC_625_WM12A18_r2_combined GAGAAGCA_1898_WM12A18_r2_combined CCTCGCGT_2428_WM12A18_r2_combined TGGGTAAA_1298_WM12A18_r2_combined TACAGTCT_1380_WM12A18_r2_combined TAGGGGAA_684_WM12A18_r2_combined CAGAACAG_55_WM12A18_r2_combined GTGTGATG_46_WM12A18_r2_combined TGACCAAT_771_WM12A18_r2_combined TTCCTACC_1338_WM12A18_r2_combined CGTCCAGT_245_WM12A18_r2_combined GAAAGATT_1486_WM12A18_r2_combined CTGGTTAG_1342_WM12A18_r2_combined CTCTCCTT_57_WM12A18_r2_combined GATTCTCC_1394_WM12A18_r2_combined ACATCGAC_559_WM12A18_r2_combined ATTTAAGT_1378_WM12A18_r2_combined AGATGGAG_1097_WM12A18_r2_combined GGGTCGTA_102_WM12A18_r2_combined TCCTTGGC_1705_WM12A18_r2_combined TTCAGGAA_60_WM12A18_r2_combined CTTTCTGA_1565_WM12A18_r2_combined GGGGGTTC_36_WM12A18_r2_combined CTTTGGGG_53_WM12A18_r2_combined TTGTCATT_68_WM12A18_r2_combined TGTGACAG_477_WM12A18_r2_combined AACACCAT_1599_WM12A18_r2_combined TCAATGTT_1081_WM12A18_r2_combined CTAAAGTC_521_WM12A18_r2_combined CGTCAGTG_682_WM12A18_r2_combined AACCCATT_176_WM12A18_r2_combined AAAGTTAA_86_WM12A18_r2_combined ACCATGTG_501_WM12A18_r2_combined TGCACAGT_44_WM12A18_r2_combined TGTTCGAT_1184_WM12A18_r2_combined TCATCACC_510_WM12A18_r2_combined GTTTTACA_1056_WM12A18_r2_combined CACTGTAT_154_WM12A18_r2_combined ATGAATGG_1983_WM12A18_r2_combined GGTCTATC_1683_WM12A18_r2_combined AGTTCGTA_1276_WM12A18_r2_combined TTCACGCC_300_WM12A18_r2_combined GCGATGTT_284_WM12A18_r2_combined TATGTGGC_1368_WM12A18_r2_combined TGAAAACG_36_WM12A18_r2_combined CTATGCTC_698_WM12A18_r2_combined GCCTTGAG_1548_WM12A18_r2_combined CAGAATAA_472_WM12A18_r2_combined GTTGGCGT_148_WM12A18_r2_combined CCCGTTTC_627_WM12A18_r2_combined ATTCTCGA_2_WM12A18_r2_combined TCCTGCGT_44_WM12A18_r2_combined CCTTAATG_196_WM12A18_r2_combined AGAGATCA_140_WM12A18_r2_combined CGGGTGTC_1653_WM12A18_r2_combined TTTGGCCT_153_WM12A18_r2_combined CGGCCATC_53_WM12A18_r2_combined GCGGTACA_346_WM12A18_r2_combined CGACTAGG_91_WM12A18_r2_combined CTTAGTAG_519_WM12A18_r2_combined ATAATTTA_1404_WM12A18_r2_combined CAAGGACA_1678_WM12A18_r2_combined ATAAAAGA_546_WM12A18_r2_combined GCTCGAGC WM12A18_r2_combined TTATAGAC_1791_WM12A18_r2_combined CCCGGTTC_74_WM12A18_r2_combined TTTCTTAC_59_WM12A18_r2_combined AGGTTTAC_56_WM12A18_r2_combined GCGATGAT_314_WM12A18_r2_combined AAACTGGG_1368_WM12A18_r2_combined CACTGGCT_913_WM12A18_r2_combined CCCAATTA_1366_WM12A18_r2_combined TACCCTTC_246_WM12A18_r2_combined GTGTCTTC_2_WM12A18_r2_combined CTTTATGT_1518_WM12A18_r2_combined ATGGTTGG_489_WM12A18_r2_combined AACCAACC_74_WM12A18_r2_combined TACGCAAT_397_WM12A18_r2_combined GTCTGGCT_1093_WM12A18_r2_combined AGCAGATG_1272_WM12A18_r2_combined GGGACATA_815_WM12A18_r2_combined GCAACGTG_1215_WM12A18_r2_combined AATCCTTT_668_WM12A18_r2_combined TCGCAAGC_484_WM12A18_r2_combined AACGAACT_1431_WM12A18_r2_combined ATCATGTA_1473_WM12A18_r2_combined CTCCAAGC_1263_WM12A18_r2_combined TAGGGCCC_281_WM12A18_r2_combined GGTAACGT_840_WM12A18_r2_combined GTATTACA_1859_WM12A18_r2_combined ATATCAGA_1594_WM12A18_r2_combined CAAGTAGA_62_WM12A18_r2_combined CAAGTGGA_1432_WM12A18_r2_combined TGTTACTC_613_WM12A18_r2_combined TATACACT_573_WM12A18_r2_combined GGAGCCGC_929_WM12A18_r2_combined TCGATCGA_39_WM12A18_r2_combined GACTTAGA_1138_WM12A18_r2_combined TGATATCG_1291_WM12A18_r2_combined CGTCGTAT_2028_WM12A18_r2_combined TGACATGC_756_WM12A18_r2_combined ACAGCCTA_42_WM12A18_r2_combined GATGGCGT_1155_WM12A18_r2_combined CCATGCAA_1572_WM12A18_r2_combined GTGTCCGA_1402_WM12A18_r2_combined CGCGATAG_39_WM12A18_r2_combined TAATGTGC_771_WM12A18_r2_combined AGGAGTAT_1323_WM12A18_r2_combined GGTTATTG_1251_WM12A18_r2_combined GTGTGGTG_867_WM12A18_r2_combined CCGTTTCA_44_WM12A18_r2_combined GGTATCAT_109_WM12A18_r2_combined TAGATATA_269_WM12A18_r2_combined TCATTATT_63_WM12A18_r2_combined TCCACCAT_46_WM12A18_r2_combined GCAGAGGC_110_WM12A18_r2_combined ACGAGTAC_828_WM12A18_r2_combined GATTGCAC_1159_WM12A18_r2_combined GTTTATTA_508_WM12A18_r2_combined TCACGGCG_753_WM12A18_r2_combined CCCGTATG_568_WM12A18_r2_combined AGGCGTCA_1099_WM12A18_r2_combined TGCGGTAT_1042_WM12A18_r2_combined CCGCTGGA_2158_WM12A18_r2_combined AGTGCTAC_1127_WM12A18_r2_combined CAAGACCT_722_WM12A18_r2_combined GATAAGTT_1441_WM12A18_r2_combined TGGTAAGA_73_WM12A18_r2_combined CCGAAAGC_1109_WM12A18_r2_combined GCAATTGG_989_WM12A18_r2_combined TATAAGTA_774_WM12A18_r2_combined TTCAGCGT_83_WM12A18_r2_combined CACACATC_1435_WM12A18_r2_combined ATTTAGCG_1235_WM12A18_r2_combined TGATTATG_37_WM12A18_r2_combined CTGTTCTA_61_WM12A18_r2_combined AGGGTCAG_1214_WM12A18_r2_combined CCCATCTC_878_WM12A18_r2_combined ACAAGATT_1064_WM12A18_r2_combined GCTTTGGT_47_WM12A18_r2_combined ATGTACCT_58_WM12A18_r2_combined CAGCAGCA_1523_WM12A18_r2_combined CCCGCAGA_360_WM12A18_r2_combined CTAGATTG_1341_WM12A18_r2_combined CTTTGCTA_72_WM12A18_r2_combined AATAACAG_1291_WM12A18_r2_combined CCATCGCG_907_WM12A18_r2_combined TATTCCGC_1233_WM12A18_r2_combined GTTTCATG_1590_WM12A18_r2_combined ACTTTCAC_1758_WM12A18_r2_combined CGATTAAT_764_WM12A18_r2_combined GCGGCATC_442_WM12A18_r2_combined CTGCTTCT_1351_WM12A18_r2_combined TGGTGCTC_1152_WM12A18_r2_combined TATTTTTT_65_WM12A18_r2_combined GATAGGAA_1291_WM12A18_r2_combined CAAGCTGC_452_WM12A18_r2_combined ACTTAAAT_4_WM12A18_r2_combined CTTGAAGT_37_WM12A18_r2_combined TACACTTA_1175_WM12A18_r2_combined TTCCGATG_1892_WM12A18_r2_combined GTCGTTAT_44_WM12A18_r2_combined CTGCGAGT_1577_WM12A18_r2_combined 0.02 AGATCCTA_3565_WM12A16_r2_combined AGTGATGG_3234_WM12A16_r2_combined AGTTGTAG_247_WM12A16_r2_combined TACCTCGG_869_WM12A16_r2_combined TAGAAAAA_3606_WM12A16_r2_combined ACTTTCCC_3937_WM12A16_r2_combined CAGTTACT_3561_WM12A16_r2_combined CCCTCATA_3511_WM12A16_r2_combined CGAGGTAC_3021_WM12A16_r2_combined CAAAAATG_850_WM12A16_r2_combined TAGAGGCT_321_WM12A16_r2_combined ACACCTAA_2946_WM12A16_r2_combinedCTATTATG_203_WM12A16_r2_combined CTAATCCG_113_WM12A16_r2_combined AAAATCGC_2731_WM12A16_r2_combined TCTCGGAT_3937_WM12A16_r2_combined GGCAGCCG_4824_WM12A16_r2_combined GCAACAAC_3799_WM12A16_r2_combinedACTTTGGG_3865_WM12A16_r2_combined CTATAATT_2967_WM12A16_r2_combined CACTTGAG_3337_WM12A16_r2_combined TAGCGCGT_135_WM12A16_r2_combined GATCGGAT_3749_WM12A16_r2_combined CTTTATCT_4497_WM12A16_r2_combined TTTAGTAT_2466_WM12A16_r2_combined GAATTTCG_912_WM12A16_r2_combined TACCTAGT_97_WM12A16_r2_combined AGTATTTA_4727_WM12A16_r2_combined AAGTATAT_87_WM12A16_r2_combined TTTGTTCA_62_WM12A16_r2_combined GAGGTGTA_3667_WM12A16_r2_combined CTGCTCGA_2595_WM12A16_r2_combined CCCCGACC_2999_WM12A16_r2_combined CTGAATTA_4350_WM12A16_r2_combined GCATAATT_2770_WM12A16_r2_combined GACGCTCC_3040_WM12A16_r2_combined GAGTTCAC_716_WM12A16_r2_combined AGTGAAGG_3020_WM12A16_r2_combined CTCGACGC_4250_WM12A16_r2_combined CGGAACTA_3615_WM12A16_r2_combined CCACAAGG_3412_WM12A16_r2_combined TCGATGCG_3119_WM12A16_r2_combined TATATGAG_2613_WM12A16_r2_combined CCAAAAGC_83_WM12A16_r2_combined GAGCGAAC_2019_WM12A16_r2_combined AGTTCGTC_278_WM12A16_r2_combined TACTTTCA_1927_WM12A16_r2_combined TAGAATGA_3856_WM12A16_r2_combined AATACTGA_3195_WM12A16_r2_combined CGACACGA_3804_WM12A16_r2_combined CGCTACAT_4039_WM12A16_r2_combined GGCATTGT_1967_WM12A16_r2_combined TCTCCGTA_3243_WM12A16_r2_combined TGGCTTTA_247_WM12A16_r2_combined TCGGAAAT_69_WM12A16_r2_combined CCCACAGC_473_WM12A16_r2_combined GGCACAAC_3419_WM12A16_r2_combined TCCCTACT_1688_WM12A16_r2_combined CGCCTGGT_3678_WM12A16_r2_combined CAGTTCGC_3749_WM12A16_r2_combinedTTCTAAGT_3153_WM12A16_r2_combined CCAATCCC_3707_WM12A16_r2_combined CAAGTGTT_1373_WM12A16_r2_combined GGGTATAA_2244_WM12A16_r2_combined ATGACACA_3430_WM12A16_r2_combined TATATTGC_4875_WM12A16_r2_combined TCGCGGCC_2994_WM12A16_r2_combined AGTGCTGA_85_WM12A16_r2_combined GAATCAGA_2516_WM12A16_r2_combined CTATGCCT_4853_WM12A16_r2_combined CAGTATTA_109_WM12A16_r2_combined ATAAACTG_3279_WM12A16_r2_combined GAAGTAGA_4068_WM12A16_r2_combined CGAAAAAC_142_WM12A16_r2_combined CTGGGCAA_280_WM12A16_r2_combined AAGCGTAA_90_WM12A16_r2_combined GTGACCCG_156_WM12A16_r2_combined GAATCGAG_150_WM12A16_r2_combined ATTTCAAA_3354_WM12A16_r2_combined GGTACACC_3909_WM12A16_r2_combined CTCTTGAG_2853_WM12A16_r2_combined TTCTACAA_115_WM12A16_r2_combined AACGCAGG_77_WM12A16_r2_combined ATAGAAGT_1977_WM12A16_r2_combined CAGATACA_3711_WM12A16_r2_combined TACTCTGA_116_WM12A16_r2_combined CTTTAGTA_4011_WM12A16_r2_combined TATAATAG_2599_WM12A16_r2_combined TCGAACGA_2715_WM12A16_r2_combined GCATAATC_272_WM12A16_r2_combined AGGCTAAA_4080_WM12A16_r2_combined TTTTGAGG_2635_WM12A16_r2_combined TTTATATT_3257_WM12A16_r2_combined TGTGATAT_3064_WM12A16_r2_combined AAAGTCCA_2653_WM12A16_r2_combined GCTCTAGC_4205_WM12A16_r2_combined TTAACAGG_3447_WM12A16_r2_combined GTGGATGT_3705_WM12A16_r2_combined TCAAAAGC_3618_WM12A16_r2_combined AACCAAAG_56_WM12A16_r2_combined ATGCAAGT_2969_WM12A16_r2_combined CATAACTA_205_WM12A16_r2_combined CGAGATGG_1736_WM12A16_r2_combined GCGTACTT_155_WM12A16_r2_combined ATCATTCA_56_WM12A16_r2_combined AATCACTA_1922_WM12A16_r2_combined GACCGGTC_1947_WM12A16_r2_combined TAGGCCAC_2612_WM12A16_r2_combined CCCTAGTC_307_WM12A16_r2_combined CATCACAG_3304_WM12A16_r2_combined TTTCAGAG_3412_WM12A16_r2_combined GCTCCTTA_3075_WM12A16_r2_combined AGCTCCTT_3512_WM12A16_r2_combined

17 Figure 1: Sample/subject information and phylogenetic analysis of env sequences using a deep sequencing approach. A. Sample and subject information includes a key for which samples were from which subjects, as well as nadir CD4 over the course of subjects care categories where available, CD4 levels at the time of sampling, and viral loads. B. Comparison of the sample analyzed versus time to viral rebound was recorded. In the majority of subjects, we were able to analyze the first sample in which virus was detected or a sample very close to rebound detection. C. A comparison of the number of the minimum number of major lineages is represented in both deep sequencing phylogenetic analysis and SGA phylogenetic analysis. D. Phylogenetic trees, created in FigTree, of the sequenced samples produced using deep sequencing with Primer ID, and presented so all samples are on the same scale. 17

18 B. CD4 Usage Assay Results A. Single Genome Amplification Phylogenetic Analysis R1 C8 90 R1 F8 C9 C10 C7 D2 70 E9 E2 D8 95 C5 C12 B2.2 B2 E12 D6 A9 E4 A6 C4 E3 H2 98 C6 B9 C3 B8 D3 98 R1 C7 R1 H11 R1 G8 E5 A2.2 R1 G10 R1 D8 R1 G9 R1 A11 99 R1 D % Infection (relative to CD4 low cells) % Infection (relative to CD4 hi /CCR5 low cells no MVC) % Infection (relative to CD4 hi /CCR5 low cells no scd4) JRCSF BAL 4059P 4059C B2B C7 C9C10 C12 D8G9 G8 H10H11 A4 A2A6 C8 D7E1 E9 E10A12 D1 G1F1 C11 D7E2 C. Coreceptor Usage Assay Results 4059C 4059P BAL NL43 B2 B2.2 C7 C9 C10 C12 D8 G9 G8 H11 A4 A2 A6 C8 D7 E1 E9 E10 A12 D1 G1 F1 F3 F5G7 D. Sensitivity to scd4 Assay Results C11 E2 F3 G9 B12F11 B4 C7E12 Controls Env Isolated from Plasma Controls R1 R2 R3 R4 R6 R7R8 R9 R G5 G1C12 D1 B12E9 B2.2 B6E9.2 G7 G9 F11 B4 C7 E12 G5 G1 C12 D1 B12 B2.2 B6 A8 E10 F4 A8E10 Controls Env Isolated from Plasma Controls R1 R2 R3 R4 R6 R7 R8 R9 R C 4051P BAL JRCSF B2 B2.2 C7 C9 C10 C12 D8 G9 G8 H11 A4 A2 A6 C8 E1 E9 E10 A12 D1 G1 F1 C11 D7 E2 F3 G7 G9 B12 F11 B4 C7 E12 G5 G1 C12 D1 AID SID A3 C3E2 Controls Env Isolated from Plasma B12 E9 B2.2 E9.2 F4 A8 E10 A3 C3 E2 H1 Controls R1 R2 R3 R4 R6 R7 R8 R9 R10 AID SID H1 AID SID

19 Figure 2: Phylogenetic analysis of env sequences and tropism determination results. A. Phylogenetic tree, created using MEGA, of the sequences of subject pre-therapy, ( ) sample and rebound sample, R1 (ᴼ) generated using SGA. Cloned and tropism-analyzed env sequences are marked with a beside each label. B-D. Tissue culture assay results using pseudotyped virus to define viral entry tropism phenotypes B. Relative infectivity of pseudoviruses (represented by red bars, while gray bars are controls) was determined using Affinofile cells induced to expressing low levels of CD4 compared to infection at high levels of CD4. Relative infectivity of all pseudotyped virus was below 2% and indicates T cell-tropism. C. Relative infectivity of pseudovirus in the presence of CCR5 antagonist Maraviroc (represented by red bars while gray bars are controls) showed nearly complete inhibition of all pseudotyped viruses and indicates usage of CCR5. D. Relative infectivity of TZM-bl cells by pseudotyped virus in the presence of soluble CD4 (represented by purple bars while gray bars are controls) showed resistance to inhibition by all pseudotype viruses, which further confirms T cell-tropic phenotype. 19

20 R1 R2 R3 R4 R5 R6 R7 R8 R9 R10

21 Figure 3: Highlighter plot analysis of env sequences Highlighter plots were generated from SGA env sequences using the Los Alamos National Laboratory Highlighter generator program. Highlighter plots compare each amplicon sequence to a master sequence designated for that patient. Differences between each sequence and the master sequence are denoted by colored marks corresponding to the location in which this variation is located as well as the nature of the mutation. Key: A: green; T: red; C: light blue; IUPAC: dark blue; Gaps: grey; Circle: Apobec; Diamond: GàA 21

22 R2 R5 R4 R3

23 R6 R7 R R9 R

24 Figure 4: Phylogenetic analysis of env sequences. Phylogenetic trees, created using MEGA, of the sequences from all samples ( ) sample generated using SGA. Cloned and tropism analyzed env sequences are marked with a beside each label. 24

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