Rodent Room Classification System
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- Ariel Harrington
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1 Rodent Room lassification System Level Rodent escr./ Examples Microbiological Status Known microflora Housing Procedure Space PPE Requirements Isolator N/A isposable yellow gown donned at facility entrance Other PPE as required by the individual labs LAM-maintained breeding colonies - rederived or direct from approved vendors. No excluded pathogens detected (barrier exclusion list). arrier facilities with PI-restricted access. Micro isolator cages. Autoclaved cages and bedding. HEPA-filtered workspace in a Level A+ procedure space within the animal facility. isposable yellow gown donned at facility entrance Gloves (required if opening cages) Irradiated diet. PI-maintained breeding colonies, or conventional rodents - rederived or direct from approved vendors. No excluded pathogens detected (barrier exclusion list). arrier facilities with restricted access Micro isolator cages. Autoclaved cages and bedding. Irradiated or extruded diet. HEPA-filtered workspace in a Level A procedure area within the animal facility. If handled outside a HEPA-filtered workspace, must be in a Level A procedure room dedicated to a defined user group. isposable yellow gown donned at facility entrance Hair cover (required for immunocompromised rodent work) Face mask (required for immunocompromised rodent work) Gloves (required if opening cages)
2 Rodent Room lassification System Level Rodent escr./ Examples PI or LAM-maintained breeding colonies - cleared through LAM quarantine, direct from approved vendors or rederived. Microbiological Status No excluded pathogens detected (general exclusion list). Housing Procedure Space PPE Requirements arrier facilities with restricted access Micro isolator cages. Autoclaved cages and bedding. Irradiated or extruded diet. HEPA-filtered workspace in a Level A procedure area within the animal facility. If handled outside a HEPAfiltered workspace, must be in a Level A procedure room dedicated to a defined user group. isposable yellow gown donned at facility entrance \ Hair cover (required for immunocompromised rodent work) Face mask (required for immunocompromised rodent work) Gloves (required if opening cages) onventional rodents - cleared through LAM quarantine, direct from approved vendors or rederived. No excluded pathogens detected (general exclusion list). Microisolator cages. Standard bedding and irradiated or extruded diet. HEPA-filtered workspace in a Level procedure area within animal facility. If handled outside a HEPAfiltered workspace, must be in a Level procedure area within the animal facility. isposable yellow gown donned at facility entrance Gloves (required if opening cages) onventional rodents - cleared through quarantine, direct from approved vendors or rederived. Higher risk of pathogen infection (e.g. returning from behavior areas, labs, imaging, open topped cages). No excluded pathogens detected (general exclusion list), but increased risk of infection. Standard cages (+/- microisolator lids). Standard bedding and irradiated or extruded diet. Level procedure space in animal facility. Laboratory procedure space outside animal facility. isposable yellow gown donned at facility entrance Gloves (required if opening cages) Rodents with confirmed pathogens Undergoing health testing and/or pathogen eradication Microisolator cages or disposable microisolator cages. Standard bedding and irradiated or extruded diet. Soiled cages, bedding and food may need to be autoclaved before disposal or cleaning. Minor procedures may be done in the animal holding room or in a Level procedure area within the animal facility. Animals must not be taken to Level A+, A, +, or procedure space. isposable gown Shoe covers Hair cover Face mask Gloves
3 arrier Exclusion List (Pending Final Approval for Testing) MOUSE Viruses: MHV, MVM, MPV, PVM, EIM, MNV, TMEV acteria: P. pneumotropica, Helicobacter spp, Parasites: Pinworms, Ectoparasites RATS Viruses: RTV, RMV, H1, KRV, RPV, SENAI, PVM Parasites: Pinworms, Ectoparasites General Exclusion List (Pending Final Approval for Testing) MOUSE Viruses: MHV, MVM, MPV, PVM, EIM, TMEV Parasites: Pinworms, Ectoparasites RATS Viruses: RTV, RMV, H1, KRV, RPV, SENAI, PVM Parasites: Pinworms, Ectoparasites
4 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover Face Mask Respirator Gloves o NOT enter this room if you have entered level,,,,, or rodent rooms today
5 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover (Recommended for Allergen ontrol) Face Mask Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter this room if you have entered level,,,, or rodent rooms today
6 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover (Required for immunocompromised rodent work; () () recommended for Allergen ontrol) Face Mask (required for immunocompromised rodent work) () () Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter this room if you have entered level,,, or rodent rooms today
7 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover (Recommended for Allergen ontrol) () () Face Mask () () Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter this room if you have entered level,, or rodent rooms today
8 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover (Recommended for Allergen ontrol) Face Mask Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter this room if you have entered level, or rodent rooms today
9 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover (Recommended for Allergen ontrol) Face Mask Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter this room if you have entered level rodent rooms today
10 LAM Rodent Room lassification: olor ode and lassification Examples Room LAM-Maintained onventional olonies with increased risk of infection Rodents with onfirmed Pathogens isposable Gown, onned at facility entrance Hair cover Face Mask Respirator (Recommended for Allergen ontrol) Gloves (Required if Opening ages) o NOT enter any other rodent rooms today
11 Authorized Personnel Only efore entering this facility, you MUST: omplete all required LAM training Secure Occupational Health and Safety clearance for entering this facility omply with all PPE requirements posted below and within the facility Questions? all LAM at For Facility, on: Upon Exiting Facility, iscard: isposable Yellow Gown Hair cover (Recommended for Allergen ontrol) Face Mask/Respirator (Respirator recommended for Allergen ontrol) () () () () Failure to comply with these requirements may result in loss of facility entrance privileges.
12 Upon Exit iscard all disposable personal protective equipment in the trash containers provided, including: Gowns Gloves Face masks/respirator Hair covers
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