Electrical cables can be tripped over, they will be taped down onto the floor with tape

Transcription

1 RISK ASSESSMENT for Cells Alive-Exploring the Science of Life The Forum 19 th September 2009 University of East Anglia, School of Biological Sciences General Risks Electrical cables can be tripped over, they will be taped down onto the floor with tape Poster boards and pull up poster displays can be unstable; they won t be left in places where they will be knocked over. The ultimate responsibility for participants lies with themselves, if adult, or with carers in the case of children. All electrical equipment has been tested for safety Activity Perceived Risks Steps to minimize risk Painting cell plates Glass slides can cause cuts, especially if they get broken will be verbally warned Warn parents that it may be unsuitable for very young children. Limit the number of children doing the activity at one time so they can be supervised effectively.

2 Giant Cell Jigsaw Could slip on the jigsaw. Floor puzzle may cause obstruction Warning will be given; children will be supervised at and told not to run on the jigsaw. Children to take shoes and socks off when on the jigsaw. Ensure the area is well laid out to allow movement of DNA Detective and DNA Models DNA necklace DNA microarray Chemicals: Tris, SDS lysis buffer, sodium acetate (S22, 24, 25), Ethanol R: 11 Highly flammable. S: 7-16 keep container tightly closed, dry and in a well ventilated space. Keep away from food stuffs, heat, sources of ignition and combustible materials. Slight risk of cuts on glass slides The mock up gene solutions contain low melt agarose (no risk), Phenylphthalein (S26, R36 R37 R38), thymolphthlein (S22 S24 S25) and methanol (R11 R23 R24 R25 R39, S7 S16 S24 S36 S37 S45). Hybridisation solution contains 1.6% (w/v) sodium hydroxide (S26 S37 S39 S45, R35) slight risk of burns. Risk of splashing in eyes. Lab coat and goggles will be worn by the participants. Full instructions are provided. The ethanol will be handled only by the instructor in charge of the activity and kept in an icebox. The mock up gene solutions will only be handled by the instructors. Advise parents of such. Goggles, lab coats and latexfree gloves will be worn by the participants when using the sodium hydroxide

3 A pile of bones Light box for x-rays will radiate heat solution. A warning sign will be placed next to the box. Sand-pit: sand may get into children's eyes if thrown Glasses/goggles will be available as well as full- time supervision. Brain waves and muscle activity Floor puzzle may cause obstruction Electrodes, purchased from Biopac Inc, contain gel and adhesive which may present a risk of an allergic reaction. The manufacturer states that the products are safe and although carry a 2% risk of reaction. Full details of their composition can be found at Details.asp?ID=180. Removal of electrodes can be uncomfortable. Burns may result from heat of the laminator. Electrical hazard from laptop and laminator. Ensure the area is well laid out to allow movement of All equipment will be under constant supervision. Instructions for the activity will be both written and verbal. Verbal permission to touch the participant will be obtained before commencing. In the event of an allergic reaction medical advice will be sort. by laminator. Fingerprints Slight risk of allergic reaction to ink Advise of risk of allergic reaction. If allergic reaction occurs seek medical advice immediately. Touch Touch box could be pulled over. Splinters from touch box. Place securely on the table Ensure children do not pull on the front. Double check all edges Sandpaper all edges properly. Abrasions to skin from sandpaper.

4 Microbes DNA Electrophoresis Cardboard cloning Microscope Collages Making slides Injury by misuse of microscope. Microscope bulb can become hot. Slight risk of cuts on glass slides Slight risk of allergic reaction to Glitterbug Nutrient agar is used for cultivating the microbes on hands. The plates are incubated at the University and images posted onto our web site. Fimo soft clay and Doudah clay will be used, the former can stain hands. Agarose DNA gels will be loaded with bromophenol blue (a dye) using a fixed volume pipette. Bromophenol Blue S: 22-24/25 Electrical hazards from electrophoresis tanks... Glass slides can cause cuts, especially if they Sign to be placed on table alongside the microscope and laptop young children will be supervised at all times. Advise participants of risk of reaction. If allergic reaction occurs seek medical advice immediately. Verbal and written instructions will be provided throughout. A lab coat and gloves will be worn, instructions will be provided. The electrophoresis equipment and powerpack has been tested for electrical safety.

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