RFE MEMO. The expiration dates for the following kit components have also been extended: A May 2021 Same as Original
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1 Page 1 of 1 RFE MEMO Product Description(s): V-PLEX and V-PLEX Plus Proinflammatory Panel 2 (rat) Kit Catalog Number(s) / Size(s): K15059-series Lot Number: K Based on additional testing, the original date of expiration for this kit has been extended by 12 months. The expiration date of K has been extended to 31 Jul The expiration dates for the following kit components have also been extended: Original Expiration Description Lot Number Date New Expiration Date Proinflammatory Panel 2 (rat) Plate Z Apr 2019 Same as Original Anti-rat IFN-γ Antibody D Apr 2019 Same as Original Anti-rat IL-1β Antibody D Apr 2019 Same as Original Anti-rat IL-4 Antibody D Jul 2018 Same as Original Anti-rat IL-5 Antibody D Jul 2018 Same as Original Anti-rat IL-6 Antibody D Apr 2019 Same as Original Anti-rat KC/GRO Antibody D Apr 2019 Same as Original Anti-rat IL-10 Antibody D Apr 2019 Same as Original Anti-rat IL-13 Antibody D Apr Jul 2018 Anti-rat TNF-α Antibody D Apr 2019 Same as Original Proinflammatory Panel 1 (rat) Calibrator Blend A May 2021 Same as Original Blocker H R Feb Jul 2018 Diluent 42 M Feb Jul 2018 Diluent 40 M Jul Jul 2018 Function Name Signature Date Quality Assurance Dawn Stollar 02 MAR 2017 This memo supersedes all other statements regarding date of expiration for this product. Memo Rev. A CM B
2 Page 1 of 5 Product Description: V-PLEX and V-PLEX Plus Proinflammatory Panel 2 (rat) Kit Kit Catalog Numbers: K15059-Series Kit Lot Number: K Expiration Date: 31 Jul 2017 Acceptance Data/ Percent Recovery Specifications: Calibrator Proinflammatory Panel 1 (rat) Control 1 Proinflammatory Panel 1 (rat) Control 2 Proinflammatory Panel 1 (rat) Control 3 Spot Name Recovery CV Recovery CV Recovery CV Recovery CV 1 rt IFN-γ 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 2 rt IL-1β 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 4 rt IL-4 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 5 rt IL-5 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 6 rt IL-6 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 7 rt KC/GRO 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 8 rt IL-10 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 9 rt IL-13 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% 10 rt TNF-α 80% - 120% < 20% 75% - 125% < 25% 75% - 125% < 25% 75% - 125% < 25% LLOQ ULOQ Spot Name Value Recovery CV Value Recovery CV 1 rt IFN-γ % - 125% < 25% % - 125% < 25% 2 rt IL-1β % - 125% < 25% % - 125% < 25% 4 rt IL % - 125% < 25% % - 125% < 25% 5 rt IL % - 125% < 25% % - 125% < 25% 6 rt IL % - 125% < 25% % - 125% < 25% 7 rt KC/GRO % - 125% < 25% % - 125% < 25% 8 rt IL % - 125% < 25% % - 125% < 25% 9 rt IL % - 125% < 25% % - 125% < 25% 10 rt TNF-α % - 125% < 25% % - 125% < 25%
3 Page 2 of 5 Acceptance Data/ Non Specific Binding Specifications: Spot Name NSB Spec 1 rt IFN-γ < 1.0% 2 rt IL-1β < 1.0% 4 rt IL-4 < 1.0% 5 rt IL-5 < 1.0% 6 rt IL-6 < 1.0% 7 rt KC/GRO < 1.0% 8 rt IL-10 < 1.0% 9 rt IL-13 < 1.0% 10 rt TNF-α < 1.0% Stock Concentration of Material Tested : Spot Name Item Conc Units Calibrator rt IFN-γ Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator rt IL-1β Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator rt IL-4 Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator rt IL-5 Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator rt IL-6 Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control rt KC/GRO Calibrator 4060
4 Page 3 of 5 7 rt KC/GRO 8 rt IL-10 9 rt IL rt TNF-α Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator 1670 Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Calibrator 1340 Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Proinflammatory Panel 1 (rat) Control Controls tested may not be the same lot as shipped.
5 Page 4 of 5 Standard Curve Data: rt IFN-γ - Spot ,530, , , , , , rt IL-1β - Spot ,220, , , , , rt IL-4 - Spot ,110, , , , , rt IL-5 - Spot ,610, , , , , rt IL-6 - Spot , , , , , rt KC/GRO - Spot ,120, , , , , , , , rt IL-10 - Spot , , , , , , rt IL-13 - Spot ,150, , , , , rt TNF-α - Spot , , , , , ,
6 Page 5 of 5 Components: Description Lot Number Storage Temperature Expiration Date Proinflammatory Panel 2 (rat) Plate Z C 30 Apr 2019 Anti-rat IFN-γ Antibody D C 31 Jul 2018 Anti-rat IL-1β Antibody D C 31 Jul 2018 Anti-rat IL-4 Antibody D C 31 Jul 2018 Anti-rat IL-5 Antibody D C 31 Jul 2018 Anti-rat IL-6 Antibody D C 30 Apr 2019 Anti-rat KC/GRO Antibody D C 30 Apr 2019 Anti-rat IL-10 Antibody D C 30 Apr 2019 Anti-rat IL-13 Antibody D C 30 Apr 2018 Anti-rat TNF-α Antibody D C 30 Apr 2019 Proinflammatory Panel 1 (rat) Calibrator Blend A C 31 May 2021 Blocker H R Room Temperature 28 Feb 2018 Diluent 42 M C 28 Feb 2018 Diluent 40 M C 31 Jul 2017 MSD Read Buffer T N/A Additional Components Included in V-PLEX Plus Plate Seals Wash Buffer (20X), 100mL Proinflammatory Panel 1 (rat) Control 1 Proinflammatory Panel 1 (rat) Control 2 Proinflammatory Panel 1 (rat) Control 3 Statement: The above product is intended for Research Use Only. Not for use in Diagnostic Procedures. Name Function Signature Date Review/Approval Dawn Stollar Quality Assurance 02 MAR 2017
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