PID Facilities in India
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1 PID Facilities in India Natinal Institute f Immunhematlgy (ICMR) 13 th Flr, NMS Building KEM Hspital Campus, Parel, Mumbai. Website: Resurce persn: 1. Dr. Manisha Madkaikar Directr-in-charge, Scientist E manishamanisha@yah.c.in Diagnstic facilities available: Primary Immundeficiency Screening: Serum Immunglbulin estimatin IgG, A, M, E - Nephelmetry-based assays Lymphcyte subset analysis: T, B and NK cells enumeratin - Flwcytmetry Activatin markers : CD25, HLA-DR - Flwcytmetry Nitrblue tetrazlium bld test - Micrscpy Severe Cmbined Immundeficiency (SCID) wrkup: Lymphcyte subset analysis: T, B and NK - Flwcytmetry T - B + NK - : CD132 (cmmn γ chain fr X- linked SCID), pstat5 - Flwcytmetry T - B + NK +: CD127 (IL7Rα)- Flwcytmetry and Mlecular study T - B - NK - : ADA - Spectrphtmeter and Mlecular study T - B - NK + : RAG1/ RAG2 gene - Mlecular study MHC class II deficiency(cd4 lymphpenia) : HLA DR expressin - Flwcytmetry Very lw CD8: CD8 α Expressin - Flwcytmetry CD8 lymphpenia: ZAP70 gene - Mlecular study
2 Chrnic Granulmatus Disease (CGD) wrkup: Nitrblue tetrazlium bld test - Micrscpy Dihydrrhdamine assay (DHR)- Flwcytmetry Autsmal recessive: p47phx, p67 phx(intracellular antigens) - Flwcytmetry CYBB, gene NCF1 gene, NCF2 gene - Mlecular study X linked 7 D5 expressin (gp91phx/p22phx) - Flwcytmetry CYBB gene - Mlecular study Hemphagcytic Lymphhisticytsis (HLH) wrkup: Perfrin and Granzyme B n NK cells and Tc cells - Flwcytmetry NK cell cyttxicity and Granule release assay- Flwcytmetry Perfrin gene (FHL2), MUNC 13-4 gene (FHL3), STX 11 gene (FHL4)- Mlecular study IL6, IL10, Ferritin, scd25, TNFα - ELISA Leukcyte Adhesin Deficiency-I (LAD- I): CD18, CD11a, CD11b, CD11c- Flwcytmetry ITBG2 (β2 integrin gene) - Mlecular study Autimmune Lymphprliferative Syndrme (ALPS) wrk up Lymphcyte subset analysis: T, B and NK- Flwcytmetry Duble negative T cells (CD3, TCRαβ, CD4, and CD8) - Flwcytmetry Apptsis assay- Flwcytmetry FAS ligand and Vit B 12 -ELISA FAS gene -Mlecular study BENTA (B-cell expansin with NF-jB and T-cell anergy) Wrkup Lymphcyte subset analysis: T, B and NK- Flwcytmetry B cell immunphentyping- Flwcytmetry Naïve and Memry B cells Switched and Unswitched B cells Switched and Unswitched memry B cells Transitinal cells Serum Immunglbulin estimatin IgG, A, M, E- Nephelmetry-based assays CARD11 gene - Mlecular study
3 Mendelian Susceptibility t Mycbacterial Diseases (MSMD) wrkup: Lymphcyte subset analysis: T, B and NK- Flwcytmetry Hyper IgM syndrme Nitrblue tetrazlium bld test -Micrscpy IFNγR1, IFNγR2 and IL-12Rβ1 expressin- Flwcytmetry pstat1, pstat4- Flwcytmetry IFNγ and IL-12P40 - ELISA IFNγR1 gene- Mlecular study Autsmal recessive: X linked CD40 expressin- Flwcytmetry CD40 gene Mlecular study Serum Immunglbulin estimatin IgG, A, M, E-Nephelmetry-based assays CD40L expressin n activated T cells Flwcytmetry CD40L gene Mlecular study Serum Immunglbulin estimatin IgG, A, M, E-Nephelmetry-based assays X-linked agammaglbulinemia (XLA) wrkup Lymphcyte subset analysis: T, B and NK- Flwcytmetry CD20 expressin- Flwcytmetry Btk expressin n mncytes- Flwcytmetry Serum Immunglbulin estimatin IgG, A, M, E -Nephelmetry-based assays
4 PID Facilities in India Bai Jerbai Wadia Hspital fr Children, Mumbai Website: Resurce persn: Dr. Mukesh Desai Hn. Hematlgist Onclgist & Immunlgist. Prf. f Pediatric Hematlgy Onclgy (DNB) Department f PHO. Chief Divisin f Immunlgy. B. J. Wadia Hspital fr Children. Cell: Tel: / Etxn mmdesai007@gmail.cm URL: URL: Mr. Prasad D. Taur Junir Scientific Officer Cell: prasadtaur@gmail.cm Bai Jerbai Wadia Hspital fr Children, Mumbai acts as a tertiary referral centre in and arund Maharashtra. Dr. Zinet Currimbhy funded the Divisin f Immunlgy at B.J. Wadia Hspital fr Children. She evlved a cllabrative apprach with NIIH that established the investigatin fr Primary Immundeficiency and Divisin f Immunlgy, B.J. Wadia Hspital, which run the clinical services. The Divisin f Immunlgy has its ut patients services n every Thursday in OPD n. 6 since 4 th OCT 2007 at Bai Jerbai Wadia Hspital fr Children, Parel, Mumbai. It als caters fr ambulatry and Indr care f Children with Primary Immundeficiency Disrders (PID).
5 The Divisin f Immunlgy has a pst f Medical Research Officer called as Currimbhy Schlar having remuneratin f 12000/- per mnth. The Divisin f Immunlgy has Mr. Prasad Taur appinted as Junir Scientific Officer. The wrk is carried ut at ur Divisin f Immunlgy and at Natinal Institute f Immunhaematlgy (NIIH), ICMR as per liaisn established with them. Facilities: Immunlgy OPD: Thursday 10:00 am t 12:00 am in OPD n.6 Immunlgy Case Discussin: Mnday 11:30 1:00 pm New Immunlgy referrals: Accepted between 9am t 4 pm between Mnday t Saturday Indr Service: Indr patients frm Medical, Surgical units and Intensive Care areas were being catered t by the Divisin f Immunlgy n a daily basis. The Divisin f Immunlgy has cllabratin in varius nging prjects n different cards f Primary Immundeficiency with Natinal Institute f Immunhaematlgy (NIIH), Entervirus Research Centre (EVRC), ICMR.
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