A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION ISOLATION OF HIGHLY PURIFIED AND INTACT EXOSOMES AND OTHER EXTRACELLULAR VESICLES
|
|
- Lee Smith
- 5 years ago
- Views:
Transcription
1 INNOVACIÓN TECNOLÓGICA PARA LABORATORIO A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION ISOLATION OF HIGHLY PURIFIED AND INTACT EXOSOMES AND OTHER EXTRACELLULAR VESICLES
2
3 A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION INTRODUCTION Exosomes are small extracellular vesicles ( nm in diameter) carrying mrna, mirna, DNA as well as various secretory proteins. [1-3] They are ubiquitous components of body fluids and can be found in blood, saliva, urine, cerebrospinal fluid (CSF) and breast milk. [4] Although their exact biological function is not yet fully characterized, increasing evidence is indicating that exosomes play a critical role in intercellular communication and thus, have a great potential as biomarkers for a variety of diseases, including cancer, [5,6] immune disorders [7] and neurodegeneration. [8] MagCapture Exosome Isolation Kit PS can be used for isolating high purity exosomes as well as other extracellular vesicles from cell culture media or body fluids. This method enables rapid and antibody-free purification and does not require usage of an ultracentrifuge. PS AFFINITY METHOD A NOVEL PURIFICATION METHOD Conventional exosome isolation methods include ultracentrifugation (UC), polymer-based precipitation and affinity purification using surface antigen targeting antibodies. [4,9] Among those, UC although most commonly used requires the purchasing of an expensive instrument and allows purification of only small amounts of extracellular vesicles. [10] In contrast, polymer-based precipitation is rather inexpensive and can recover large amounts of exosomes. [11] Nonetheless, the purity of the isolated fractions is comparably low making this method unsuitable for most downstream applications. Affinity-based purification strategies use an antibody directed against exosome-specific surface proteins, such as CD9, CD63 or CD81. [12,13] Hence, these methods usually lead to a high purity of isolated fractions. One limitation, however, is the necessity of using stringent conditions to detach the antibodies from the purified exosomes. As a consequence, the isolated material is often damaged during the elution step, thus, making it unsuitable for downstream applications. MagCapture Exosome Isolation Kit PS has been recently introduced as an affinity-based, yet antibodyfree, purification method. It uses magnetic beads coupled to a phosphatidylserine (PS)-binding protein which recognizes and binds exosomes in a calcium-dependent manner. The elution of exosomes and extracellular vesicles can, therefore, be performed at neutral ph by simply adding a metal-chelating reagent. The intact exosomes isolated by means of this new purification method are suited for various applications including electron microscopy, nanoparticle tracking analysis and analysis of intra-vesicle proteins, lipids or nucleic acids. Metal ion Magnetic beads PS-binding protein Chelating agent Magnetic beads PS-binding protein Extracellular vesicle Intact extracellular vesicle Calcium-dependent capture of exosomes and extracellular vesicles 2
4 A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION COMPARISON WITH CONVENTIONAL METHODS The main advantage of the PS affinity method is the remarkably high purity and the high recovery of the isolated exosomes and extracellular vesicles. Conventional purification methods show lower performance results in terms of purity and material recovery. METHOD EXOSOME PURITY EXOSOME RECOVERY INTACT VESICLES RECOVERY PS affinity method Ultracentrifugation Polymer-based precipitation Exosome surface antigen affinity method (using antibodies) Very low Low Medium High Very High Yes Yes Yes No Comparison of the PS affinity method with conventional isolation methods The most commonly used method to purify extracellular vesicles is ultracentrifugation (UC). This purification method is time-consuming, cost-intensive and ineffective in terms of material recovery. Polymer-based precipitation, on the other hand, efficiently recovers exosomes, but is prone to contamination by non-exosomal particles and is, therefore, often not suitable for downstream applications. Another widely used method is the affinity purification using antibodies directed against exosomespecific surface proteins. Latter strategy, however, also suffers from poor compatibility with downstream applications, as harsh conditions are usually required to detach the purified vesicles from the antibody which often damage the isolated material. The newly developed PS affinity method is based on magnetic beads coated with a phosphatidylserine (PS)-binding receptor protein. Recognition of exosomes and extracellular vesicles occurs in a calciumdependent manner, thereby enabling easy elution of purified material using mild chelating agents. Hence, the PS affinity method allows an easy and high yielding purification of intact exosomes which can be further submitted to numerous downstream applications. MagCapture Exosome Isolation Kit PS Ultracentrifugation Polymer-based precipitation Electron microscopy pictures of extracellular vesicles purified by PS affinity method, UC and polymer-based precipitation 3
5 A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION MAGCAPTURE EXOSOME ISOLATION KIT PS PROTOCOL Sample pretreatment Cell culture medium Serum, plasma or urine Centrifugation Concentration (optional) Exosome binding enhancer (x500) Affinity reaction (180 min.) Incubation with functionalized magnetic beads Exosome elution buffer Washing buffer Exosome binding enhancer (x500) Washing & elution (15 min.) High purity and intact exosome Exosomes and extracellular vesicles isolation protocol using PS affinity method 4
6 A NOVEL AFFINITY-BASED METHOD FOR EXOSOME ISOLATION REFERENCES [1] M. Colombo, G. Raposo, C. Thery, Annu Rev Cell Dev Biol. 2014, 30, [2] M. Tkach, C. Thery, Cell. 2016, 164, [3] E. M. Kramer-Albers, A. F. Hill, Curr Opin Neurobiol. 2016, 39, [4] W. Nakai, T. Yoshida, D. Diez, Y. Miyatake, T. Nishibu, N. Imawaka, K. Naruse, Y. Sadamura, R. Hanayama, Sci Rep. 2016, 6, [5] J. Skog, T. Wurdinger, S. van Rijn, D. H. Meijer, L. Gainche, M. Sena-Esteves, W. T. Curry, Jr., B. S. Carter, A. M. Krichevsky, X. O. Breakefield, Nat Cell Biol. 2008, 10, [6] M. Baj-Krzyworzeka, R. Szatanek, K. Weglarczyk, J. Baran, B. Urbanowicz, P. Branski, M. Z. Ratajczak, M. Zembala, Cancer Immunol Immunother.: CII 2006, 55, [7] H. Valadi, K. Ekstrom, A. Bossios, M. Sjostrand, J. J. Lee, J. O. Lotvall, Nat Cell Biol. 2007, 9, [8] F. Raimondo, L. Morosi, C. Chinello, F. Magni, M. Pitto, Proteomics 2011, 11, [9] P. Li, M. Kaslan, S. H. Lee, J. Yao, Z. Gao, Theranostics 2017, 7, [10] C. Thery, S. Amigorena, G. Raposo, A. Clayton, Curr Protoc Cell Biol. 2006, Chapter 3, Unit [11] M. A. Rider, S. N. Hurwitz, D. G. Meckes, Sci Rep. 2016, 6, [12] L. Balaj, N. A. Atai, W. Chen, D. Mu, B. A. Tannous, X. O. Breakefield, J. Skog, C. A. Maguire, Sci Rep. 2015, 5, [13] A. Ghosh, M. Davey, I. C. Chute, S. G. Griffiths, S. Lewis, S. Chacko, D. Barnett, N. Crapoulet, S. Fournier, A. Joy, M. C. Caissie, A. D. Ferguson, M. Daigle, M. V. Meli, S. M. Lewis, R. J. Ouellette, PloS One 2014, 9, e PRODUCT DETAILS TTTTT PRODUCT NAME PACKAGE SIZE ARTICLE NO. STORAGE MagCapture Exosome Isolation Kit PS 2 Tests Tests Keep at 2-10 C 5
7
8 www. rafer. es Barcelona Bilbao La Coruña Madrid Málaga Sevilla Valencia Zaragoza Lisboa
Comercial Rafer in Zaragoza
Comercial Rafer in Zaragoza 06.04.2017 ~A novel affinity method to isolate and identify intact Exosomes~ Taku Funakoshi (Mr.) Wako Chemicals GmbH Laboratory Chemicals Division Manager Research fields of
More informationMagCapture Exosome Isolation Kit PS Q&A
MagCapture Exosome Isolation Kit PS Q&A Specifications and performance P.1 Comparison of the conventional method P.2 Operation methods and composition P.4 Amount of starting sample P.5 Analysis after exosomes
More informationvol.3 Highlight Product: ExoCap Product Research tools for extracellular vesicles - Exhibition booth at Taiwan on November
vol.3 Feb. 2018 Highlight Product: ExoCap Product Research tools for extracellular vesicles - Exhibition booth at Taiwan on November Exhibition / Webinar on March 2018 is a big year! CELEBRATE MBL 50th
More informationPRODUCT INFORMATION & MANUAL
PRODUCT INFORMATION & MANUAL 0.4 micron for Overall Exosome Isolation (Cell Media) NBP2-49826 For research use only. Not for diagnostic or therapeutic procedures. www.novusbio.com - P: 303.730.1950 - P:
More informationExtracellular Vesicle RNA isolation Kits
Extracellular Vesicle RNA isolation Kits Summary Section 4 Introduction 42 Exo-TotalRNA and TumorExo-TotalRNA isolation kits 43 Extracellular Vesicle RNA extraction kits Ordering information Products can
More informationExosomes Immunocapture and Isolation Tools: Immunobeads
Product Insert Exosomes Immunocapture and Isolation Tools: Immunobeads HBM-BOLF-##/##-# HBM-BOLC-##/##-# HBM-BTLF-##/##-# Overall Exosome capture from Human Biological fluids Overall Exosome capture from
More informationEXO-Prep One step Exosome isolation Reagent
Datasheet EXO-Prep One step Exosome isolation Reagent This product is for research use only. It is highly recommended to read this users guide in its entirety prior to using this product. Do not use this
More informationA new approach to characterize exosomes and study their interactions with biomolecules. Silvia Picciolini PhD student 30 th May 2016
A new approach to characterize exosomes and study their interactions with biomolecules Silvia Picciolini PhD student 30 th May 2016 Extracellular vesicles (EVs) Most body cells release physiologically
More informationService and Collaboration
Summary Section 7 Introduction 68 Exosome and Microvesicle Isolation 69 EV protein quantification, sceening and profiling 69 Nanoparticle Tracking Analysis (NTA) 70 EV Nucleic Acid isolation 70 Nucleic
More informationUSE OF EXOSOMES IN TRANSLATIONAL AND CLINICAL RESEARCH. Eva Colás Ortega Postdoc researcher IRBLleida
USE OF EXOSOMES IN TRANSLATIONAL AND CLINICAL RESEARCH Eva Colás Ortega Postdoc researcher IRBLleida ecolas@irblleida.cat THERE ARE DIFFERENT TYPE OF VESICLES RELEASED BY CELLS MICROVESICLES Size: 100-1000nm
More informationExosome DNA Extraction Kits
Exosome DNA Extraction Kits Summary Section 5 Introduction 40 EXO-DNAc 41 EXO-DNA 43 Introduction Genomic DNA Extractiom Kits Ordering informations Products can be purchased directly in our on-line shop:
More informationEXOSOMES & MICROVESICLES
The Sample Preparation Experts EXOSOMES & MICROVESICLES The New Standard in Exosome Purification and RNA Isolation Best-in-Class, Pure & Simple Exosome Purification, Fractionation of Exosomal Free & Circulating
More informationExo-spin Exosome Purification Kit For cell culture media/urine/saliva and other low-protein biological fluids
Exo-spin Exosome Purification Kit For cell culture media/urine/saliva and other low-protein biological fluids Cat EX01 Protocol Version 1.1 1 Contents 1. Storage 3 2. Kit contents 3 3. Product Information
More informationExosome detection and characterization based on flow cytometry.
Exosome detection and characterization based on flow cytometry. Ricardo Jara Acevedo 1, Carmen Campos Silva 2, María del Yáñez Mó María 3, Mar Vales Gómez 2. 1. ImmunoStep SL. Salamanca. Spain. 2. Department
More informationEXO-Prep. Product Insert. Kit for exosome isolation from biofluids or cell culture supernatants HBM-EXP-##
Product Insert HBM-EXP-## Kit for exosome isolation from biofluids or cell culture supernatants This product is for research use only. It is highly recommended to read this users guide in its entirety
More informationExo-spin Exosome Purification Kit For cell culture media/urine/saliva and other low-protein biological fluids
User Guide Exo-spin Exosome Purification Kit For cell culture media/urine/saliva and other low-protein biological fluids Cat EX01 Protocol Version 5.6 Contents 1. Storage 3 2. Product Components 3 3. Product
More informationmirna in Plasma Exosome is Stable under Different Storage Conditions
Molecules 2014, 19, 1568-1575; doi:10.3390/molecules19021568 Article OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules mirna in Plasma Exosome is Stable under Different Storage Conditions
More informationExtracellular Vesicle Quantification Kits
Summary Section 3 Introduction 34 ExoTEST TM Ready to Use ELISA quantification kit 35 Exo-FACS Ready to Use kit 38 Ordering information Exosome research is a relatively novel and exciting field of biomedical
More informationExosomes Immunocapture and Isolation Tools: Immunoplates
Product Insert Exosomes Immunocapture and Isolation Tools: Immunoplates HBM-POF-##/## HBM-POC-##/## Overall Exosome capture from Biological fluids (plasma, urine) Overall Exosome capture from Cell culture
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-cfDNA Serum & Plasma Kit Catalog No. D4076 Highlights High-quality DNA, including cell-free, is easily and robustly purified from up to 10 ml of serum/plasma, up to 1 ml amniotic
More informationExo-spin Blood Exosome Purification Kit For blood sera/plasma
User Guide Exo-spin Blood Exosome Purification Kit For blood sera/plasma Cat EX02 Protocol Version 5.6 Contents 1. Storage 3 2. Product Components 3 3. Product Information 4 4. General Information 4 5.
More informationEXO-DNAc Circulating and EV-associated DNA extraction kit
Datasheet EXO-DNAc Circulating and EV-associated DNA extraction kit This product is for research use only. It is highly recommended to read this users guide in its entirety prior to using this product.
More informationExiPrep TM Viral DNA/ RNA Kit
Ver. 2.0 ExiPrep TM Viral DNA Kit (K-3515) ExiPrep TM Viral RNA Kit (K-3525) (K-3535) Safety Warnings and Precautions is developed and sold for research purposes only. It is not recommended for human or
More informationExoQuick Exosome Isolation and RNA Purification Kits
ExoQuick Exosome Isolation and RNA Purification Kits Cat # EQ806A-1, EQ806TC-1, EQ808A-1 User Manual Storage: Please see individual components Version 2 8/14/2018 A limited-use label license covers this
More informationEXO-DNA Circulating and EV-associated DNA extraction kit
Datasheet EXO-DNA Circulating and EV-associated DNA extraction kit This product is for research use only. It is highly recommended to read this users guide in its entirety prior to using this product.
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-cfDNA/cfRNA Serum & Plasma Kit Catalog No. R1072 Highlights High-quality cell-free DNA and RNA are easily and robustly purified from up to 3 ml of plasma, serum, or other biological
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Viral Kit Catalog Nos. R1034 & R1035 Highlights Quick, spin-column purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood,
More informationEXOTESTTM. ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids
DATA SHEET EXOTESTTM ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids INTRODUCTION Exosomes are small endosome-derived lipid nanoparticles
More informationAutomated pull-down of extracellular vesicles (EVs) on the KingFisher system using Dynabeads magnetic beads standardizing EV capture and analysis
PPLICTION NOTE KingFisher Flex Purification System utomated pull-down of extracellular vesicles (EVs) on the KingFisher system using Dynabeads magnetic beads standardizing EV capture and analysis xl. Neurauter,
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-DNA/RNA Viral MagBead Catalog Nos. R2140 & R2141 Highlights High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media,
More informationUrine Exosome RNA Isolation Kit Product #47200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Urine Exosome RNA Isolation Kit Product #47200 Product Insert
More informationInsight into cancer research from discovery to validation
Insight into cancer research from discovery to validation 1 Exosomes in cancer research: from exosome isolation to biomarker discovery Zheng Songyue, PhD Technical Sales Specialists Life Technologies The
More informationProduction of Exosomes in a Hollow Fiber Bioreactor
Production of Exosomes in a Hollow Fiber Bioreactor John J S Cadwell, President and CEO, FiberCell Systems Inc INTRODUCTION Exosomes are small lipid membrane vesicles (80-120 nm) of endocytic origin generated
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-DNA/RNA Pathogen Miniprep Catalog Nos. R1042 & R1043 Highlights Spin-column purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes,
More informationXTRAKT FFPE Kit / Manual
XTRAKT FFPE Kit / Manual For manual extraction of RNA, mirna and/or DNA from Formalin Fixed Paraffin Embedded (FFPE) tissue samples Catalog No. # XTK2.0-96 For research use only. Not intended for diagnostic
More informationCaution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard. Product Number: AD0013
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard Product Number: AD0013 INTRODUCTION: Fluorescent isothiocyanato-activated
More informationCONTRACTING ORGANIZATION: Southern Illinois University School of Medicine, Springfield, IL
Award Number: W81XWH-14-1-0019 TITLE: Identification and Reconstruction of Prostate Tumor-Suppressing Exosomes for Therapeutic Applications PRINCIPAL INVESTIGATOR: Daotai Nie CONTRACTING ORGANIZATION:
More informationLANCE Eu-W1024 ITC Chelate & Europium Standard AD0013 Development grade
AD0017P-4 (en) 1 LANCE Eu-W1024 ITC Chelate & Europium Standard AD0013 Development grade INTRODUCTION Fluorescent isothiocyanato-activated (ITC-activated) Eu-W1024 chelate is optimized for labelling proteins
More informationINSTRUCTION MANUAL. RNA Clean & Concentrator -5 Catalog Nos. R1015 & R1016. Highlights. Contents
INSTRUCTION MANUAL Catalog Nos. R1015 & R1016 Highlights Quick (5 minute) method for cleaning and concentrating RNA. Ideal for purification of RNA from aqueous phase following an acid phenol extraction.
More informationBioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:
Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions
More informationCaution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard. Product Number: AD0014
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard Product Number: AD0014 INTRODUCTION: Iodoacetamido-activated
More informationExoQuick PLUS Exosome Purification Kit for Serum & Plasma
ExoQuick PLUS Exosome Purification Kit for Serum & Plasma Cat# EQPL10A-1 User Manual Store kit at +4 0 C Version 2 2/22/2017 A limited-use label license covers this product. By use of this product, you
More informationProduct Contents. 1 Specifications 1 Product Description. 2 Buffer Preparation... 3 Protocol. 3 Ordering Information 4
INSTRUCTION MANUAL Quick-RNA Midiprep Kit Catalog No. R1056 Highlights 10 minute method for isolating RNA (up to 1 mg) from a wide range of cell types and tissue samples. Clean-Spin column technology allows
More informationProduct Contents... 1 Specifications... 1 Product Description... 2 Buffer Preparation... 3 Protocol Appendix... 5 Ordering Information...
INSTRUCTION MANUAL Quick-DNA/RNA Pathogen MagBead Catalog Nos. R2145 & R2146 Highlights High-throughput, magnetic-bead based purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety
More informationSerum exosomes: A liquid biopsy for neurological disease. Michael Buckland RPAH Neuropathology Sydney, Australia
Serum exosomes: A liquid biopsy for neurological disease Michael Buckland RPAH Neuropathology Sydney, Australia Extracellular vesicles Tiny membrane-bound nano-sized vesicles Exosomes (50-100 nm) Microparticles
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / CD81)
Version 1 Last updated 26 September 2018 ab239682 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / For the isolation and analysis of exosome from cell culture. This product is
More informationExiPrep Plus Viral DNA/RNA Kit User s Guide ExiPrep Plus Viral DNA/RNA Kit ExiPrep Plus Viral DNA Kit ExiPrep Plus Viral RNA Kit
User s Guide ExiPrep Plus Viral DNA/RNA Kit ExiPrep Plus Viral DNA Kit ExiPrep Plus Viral RNA Kit K-4271 K-4272 K-4273 ExiPrep Plus Viral DNA/RNA Kit (K-4271) ExiPrep Plus Viral DNA Kit (K-4272) ExiPrep
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Miniprep Kit Catalog Nos. R1054 & R1055 Highlights High-quality total RNA (including small RNAs) from a wide range of samples. You can opt to isolate small and large RNAs in
More informationEFFECTS OF HEMATURIA ON THE PROTEOMIC PROFILE OF URINARY
Supporting Information EFFECTS OF HEMATURIA ON THE PROTEOMIC PROFILE OF URINARY EXTRACELLULAR VESICLES: TECHNICAL CHALLENGES. #Francesca Raimondo 1*, #Clizia Chinello 1, Martina Stella 1, Lucia Santorelli
More informationPinpoint Slide RNA Isolation System II Catalog No. R1007
INSTRUCTION MANUAL Pinpoint Slide RNA Isolation System II Catalog No. R1007 Highlights Allows for the isolation of total RNA from paraffin-embedded tissue sections on glass slides Simple procedure combines
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Miniprep Kit Catalog Nos. R1054 & R1055 Highlights High-quality total RNA (including small RNAs) from a wide range of samples. You can opt to isolate small and large RNAs in
More informationApplication of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma
Excerpt from MACS&more Vol 8 1/2004 Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma L. Davis Lupo and Salvatore T. Butera HIV and Retrovirology Branch,
More informationLong term stability of isolated human serum derived exosomes
Long term stability of isolated human serum derived exosomes Candice de Boer (PhD student) Regenerative Medicine Laboratory Supervisor: Associate Professor Neil Davies Exosomes First discovered during
More informationProduct Contents. 1 Specifications 1 Product Description. 2 Buffer Preparation... 3 Protocol. 3 Ordering Information 4 Related Products..
INSTRUCTION MANUAL Quick-RNA MidiPrep Catalog No. R1056 Highlights 10 minute method for isolating RNA (up to 1 mg) from a wide range of cell types and tissue samples. Clean-Spin column technology allows
More informationHDL Purification Kit (Ultracentrifugation Free)
Product Manual HDL Purification Kit (Ultracentrifugation Free) Catalog Number STA- 607 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are submicroscopic particles
More informationFor Reseach Use Only. ExoCap TM. Product Catalog. Research tools for extracellular vesicles
For Reseach Use Only ExoCap TM Product Catalog Research tools for extracellular vesicles Table of contents What are exosomes? P. 2 ExoCap Ultracentrifugation/Storage Booster (ExoCap USB) P. 3 User Application
More informationDELFIA Tb-N1 DTA Chelate & Terbium Standard
AD0029P-1 (en) 1 DELFIA Tb-N1 DTA Chelate & AD0012 Terbium Standard For Research Use Only INTRODUCTION DELFIA Tb-N1 DTA Chelate is optimized for the terbium labeling of proteins and peptides for use in
More informationBabyBio IMAC columns DATA SHEET DS
BabyBio IMAC columns DATA SHEET DS 45 655 010 BabyBio columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged)
More informationDELFIA Eu-DTPA ITC Chelate & Europium Standard
AD0026P-3 (en) 1 DELFIA Eu-DTPA ITC Chelate & AD0021 Europium Standard For Research Use Only INTRODUCTION DELFIA Eu-DTPA ITC Chelate is optimized for the europium labelling of proteins and peptides for
More informationDELFIA Tb-DTPA ITC Chelate & Terbium Standard
AD0035P-2 (en) 1 DELFIA Tb-DTPA ITC Chelate & AD0029 Terbium Standard For Research Use Only INTRODUCTION DELFIA Tb-DTPA ITC Chelate is optimized for the terbium labelling of proteins and peptides for use
More information*Please direct any requests or inquiries to the contact information provided below. Research Inquiries
Press Release August 9, 2018 Keio University School of Medicine Tokyo Medical University JVCKENWOOD Corporation Japan Agency for Medical Research and Development Development of a Highly Sensitive Device
More informationA two-microrna signature in urinary exosomes for diagnosis of prostate cancer
Poster # B4 A two-microrna signature in urinary exosomes for diagnosis of prostate cancer Anne Karin Ildor Rasmussen 1, Peter Mouritzen 1, Karina Dalsgaard Sørensen 3, Thorarinn Blondal 1, Jörg Krummheuer
More informationExosomes, Extracellular Vesicles A Technology Review
Exosomes, Extracellular Vesicles A Technology Review 1 Author, Data Collection Date, Notes The Author Gary M. Oosta, holds a Ph.D. in Biophysics from Massachusetts Institute of Technology and a B.A. in
More information155 S. Milpitas Blvd., Milpitas, CA USA T: (408) F: (408)
ExoQuant TM Tumor-derived Exosome enrichment and Quantification Assay Kit (biological fluids & cell media, luminometric) (Catalog # K129-1; 1 assays (96 well transparent immunoplate for luminometric readings;
More informationABIOpure TM Viral (version 2.0)
ABIOpure TM Viral (version 2.0) DNA/RNA Extraction Handbook Cat No: M561VT50 FOR RESEARCH USE ONLY Table of Contents Contents Page Kit Components 3 Precautions 3 Stability & Storage 4 General Description
More informationToxicity analysis of PbSQDs using nano-sized vesicles (exosome) secretedfrom HEK293 cells
Toxicity analysis of PbSQDs using nano-sized vesicles (exosome) secretedfrom HEK293 cells EunjooKim, Ph. D. Daegu GyeongbukInstitute of Science and Technology (DGIST), Republic of Korea Quantumdots RUSNANO
More informationAnnexin V-APC/7-AAD Apoptosis Kit
Annexin V-APC/7-AAD Apoptosis Kit Catalog Number KA3808 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
More informationAnnexin V-PE Apoptosis Detection Kit
Annexin V-PE Apoptosis Detection Kit Catalog Number KA0716 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationExo-Glow TM Exosome Labeling Kits
Exo-Glow TM Exosome Labeling Kits Cat# EXOR100A-1 Cat# EXOG200A-1 Cat# EXOC300A-1 User Manual Store kit at -20 o C on receipt Version 8 3/10/2017 A limited-use label license covers this product. By use
More informationPREPARED FOR: U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland
Award Number: W81XWH-16-1-0200 TITLE: A Universal Platform for Identification of Novel Lung Cancer Biomarkers Based on Exosomes PRINCIPAL INVESTIGATOR: Utkan Demirci, Ph.D CONTRACTING ORGANIZATION: Leland
More informationCanonical and selective approaches in exosome purification and their implications for diagnostic accuracy
Review Article Canonical and selective approaches in exosome purification and their implications for diagnostic accuracy Milica Popović 1, Ario de Marco 2 1 Department of Biochemistry, Faculty of Chemistry,
More informationConcentration Estimation from Flow Cytometry Exosome Data Protocol
Concentration Estimation from Flow Cytometry Exosome Data Protocol 1. STANDARD CURVE Create a standard curve for the target exosome by plotting the mean fluorescence (y axis) against the protein concentration
More informationExosome Standards. Product Insert. Lyophilized Exosome Standards from Cell culture supernatants. Lyophilized Exosome Standards from Human Biofluids
Product Insert Exosome Standards Lyophilized Exosome Standards from Cell culture supernatants Lyophilized Exosome Standards from Human Biofluids This product is for research use only. It is highly recommended
More informationMidi Plant Genomic DNA Purification Kit
Midi Plant Genomic DNA Purification Kit Cat #:DP022MD/ DP022MD-50 Size:10/50 reactions Store at RT For research use only 1 Description: The Midi Plant Genomic DNA Purification Kit provides a rapid, simple
More informationYoungnam Cho. National Cancer Center Biomarker Branch
Youngnam Cho National Cancer Center Biomarker Branch Contents 1. Liquid Biopsy 2. Circulating Tumor Cells from Blood 3. Cell-free DNA from Blood 1. Liquid biopsy Cancer Diagnosis IMAGING TISSUE BIOPSY
More informationE.Z.N.A. SQ Blood DNA Kit II. Table of Contents
E.Z.N.A. SQ Blood DNA Kit II Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Blood Storage and DNA Yield...4 Preparing Reagents...5 100-500 μl Whole Blood Protocol...6
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma
Version 1 Last updated 25 May 2018 ab228565 Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma For the isolation/detection of exosomes from human plasma, urine or cell culture media. This product
More informationHis n. Vivapure Metal Chelate Mega spin columns. Technical data and operating instructions. For in vitro use only.
His n Vivapure Metal Chelate Mega spin columns Technical data and operating instructions. For in vitro use only. columns - for the purification of proteins with poly-histidine tags Storage conditions columns
More informationThe effect of temperature and incubation time on the analysis of highly sialylated glycans from bovine fetuin
APPLICATION NOTE GlycanAssure Glycan Analysis and Quantitation System The effect of temperature and incubation time on the analysis of highly sialylated glycans from bovine fetuin Abstract This application
More informationPRODUCT INFORMATION & MANUAL
PRODUCT INFORMATION & MANUAL Overall Exosome Capture and Quantification (Plasma, Colorimetric) Kit NBP2-49782 For research use only. Not for diagnostic or therapeutic procedures. www.novusbio.com - P:
More informationFor purification of viral DNA and RNA from a wide range of sample materials
QIAamp virus kits For purification of viral DNA and RNA from a wide range of sample materials Automatable on QIAGEN s proven QIAamp Kits set the standard for purification of viral DNA and RNA. QIAamp virus
More informationCytomegalovirus (CMV) End-Point PCR Kit Product# EP36300
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cytomegalovirus (CMV) End-Point PCR Kit Product# EP36300 Product
More informationXCF TM COMPLETE Exosome and cfdna Isolation Kit (for Serum & Plasma)
XCF TM COMPLETE Exosome and cfdna Isolation Kit (for Serum & Plasma) Cat# XCF100A-1 User Manual Store kit components at +4ºC and +25ºC Version 1 2/2/2017 A limited-use label license covers this product.
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture
Version 1 Last updated 14 March 2018 ab228564 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture For the measurement of human exosomes in cell culture. This product is for research use only
More informationManual (Second edition)
Reagent for RNA Extraction ISOGENⅡ Manual (Second edition) Code No. 311-07361 Code No. 317-07363 NIPPON GENE CO., LTD. Table of contents I Product description 1 II Product content 1 III Storage 1 IV Precautions
More informationPlasma Membrane Protein Extraction Kit
ab65400 Plasma Membrane Protein Extraction Kit Instructions for Use For the rapid and sensitive extraction and purification of Plasma Membrane proteins from cultured cells and tissue samples. This product
More informationMeasuring Exosome Stability with Nanoparticle Tracking Analysis
Measuring Exosome Stability with Nanoparticle Tracking Analysis PARTICLE CONCENTRATION PARTICLE SIZE Introduction The study of extracellular vesicles is an area that has become the subject of intense study
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationInstructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
More informationA complete next-generation sequencing workfl ow for circulating cell-free DNA isolation and analysis
APPLICATION NOTE Cell-Free DNA Isolation Kit A complete next-generation sequencing workfl ow for circulating cell-free DNA isolation and analysis Abstract Circulating cell-free DNA (cfdna) has been shown
More informationSupplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS)
Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS) and their exosomes (EXO) in resting (REST) and activated
More informationLDL/VLDL Purification Kit (Ultracentrifugation Free)
Product Manual LDL/VLDL Purification Kit (Ultracentrifugation Free) Catalog Number STA- 606 10 preps FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipoproteins are submicroscopic
More informationSeparation of HDL Particles by Immunoprecipitation
Sun Diagnostics, LLC Separation of HDL Particles by Immunoprecipitation Rae-Anne Nguyen and John H. Contois 13 Introduction We all know that HDL cholesterol concentration is inversely associated with coronary
More informationencouraged to use the Version of Record that, when published, will replace this version. The most /BSR
Bioscience Reports: this is an, not the final Version of Record. You are encouraged to use the Version of Record that, when published, will replace this version. The most up-to-date version is available
More informationFast and Easy Isolation of T Cells
Fast and Easy Isolation of T Cells Isolate T Cells In As Little As 25 Minutes Isolate whole T cell populations as well as various T cell subsets with high purity and recovery using the fast and easy T
More informationRNA/DNA Stabilization Reagent for Blood/Bone Marrow
For general laboratory use. Not for use in diagnostic procedures. FOR IN VITRO USE ONLY. RNA/DNA Stabilization Reagent for Blood/Bone Marrow For simultaneous cell lysis and stabilization of nucleic acids
More informationIsolation of Exosomes for Subsequent mrna, MicroRNA, and Protein Profiling
Chapter 13 Isolation of Exosomes for Subsequent mrna, MicroRNA, and Protein Profiling Sweta Rani, Keith O Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek W. Radomski, John Crown, and
More informationWork-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples:
Dr. Sanjeeva Srivastava IIT Bombay Work-flow: protein sample preparation Precipitation methods Removal of interfering substances Specific examples: Sample preparation for serum proteome analysis Sample
More information