Intracomplex general acid/base catalyzed cleavage of RNA phosphodiester bonds: the leaving group effect. Supporting information
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1 Intracomplex general acid/base catalyzed cleavage of RNA phosphodiester bonds: the leaving group effect Supporting information Tuomas Lönnberg* and Mikko Luomala Department of Chemistry, University of Turku Vatselankatu 2, FIN Turku, Finland S1
2 Contents 1 H NMR spectrum of N 2 -{2-[N 4,N 7,N 10 -tris(tert-butoxycarbonyl)-1,4,7,10-tetraazacyclododecan-1-yl]ethyl}-n 4,N 6 -dimethylmelamine (1) S3 13 C NMR spectrum of N 2 -{2-[N 4,N 7,N 10 -tris(tert-butoxycarbonyl)-1,4,7,10-tetraazacyclododecan-1-yl]ethyl}-n 4,N 6 -dimethylmelamine (1) S6 1 H NMR spectrum of N 2 -[2-(1,4,7,10-tetraazacyclododecan-1-yl)ethyl]-N 4,N 6 -dimethylmelamine (2) S10 13 C NMR spectrum of N 2 -[2-(1,4,7,10-tetraazacyclododecan-1-yl)ethyl]-N 4,N 6 -dimethylmelamine (2) S12 UV spectrum of N 2 -[2-(1,4,7,10-tetraazacyclododecan-1-yl)ethyl]-N 4,N 6 -dimethylmelamine (2) Figure S1: HPLC chromatogram of N 2 -[2-(1,4,7,10-tetraazacyclododecan-1-yl)ethyl]-N 4,N 6 -dimethylmelamine (2) S15 S16 1 H NMR spectrum of Uridine-3 -(2-cyanoethyl)phosphate (4c) S17 13 C NMR spectrum of Uridine-3 -(2-cyanoethyl)phosphate (4c) S21 31 P NMR spectrum of Uridine-3 -(2-cyanoethyl)phosphate (4c) S24 UV spectrum of Uridine-3 -(2-cyanoethyl)phosphate (4c) S26 S2
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16 Figure S1 HPLC chromatogram of compound 2 [Thermo ODS hypersil column ( mm, 5 m); flow rate = 1.0 ml min -1 ; 60 mmol L -1 acetate buffer (ph = 4.3) and a linear gradient of 0 to 40% of MeCN during 30 min, after which 40% MeCN for 10 min; = 250 nm]. S16
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