CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS"

Transcription

1 CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS 8.1 INTRODUCTION Medicinal plants are the backbone of traditional medicine and the antibacterial activity of plant extract is due to different chemical agent in the extract, which was classified as active antimicrobial compound (Doughari et al., 2007; Kumar et al., 2009; Ignacimuthu et al., 2009; Adegoke and Adebayo-tayo, 2009). In recent years, pharmaceutical companies have been doing phytochemical research and investing billions of dollars in developing natural remedies to produce drugs in affordable price to general population (Doughari, 2006). The rising incidence in multidrug resistance amongst pathogenic microbes has further necessitated the need to search for fewer antibiotic sources from plants (Mohamed Khadeer Ahamed et al., 2007). Costus igneus is traditionally used in India to control diabetes which is also known as fiery costus or spiral flag or insulin plant are rich in protein(18%), iron(40mg) and antioxidant components such as ascorbic acid, β-carotene, α- tocopherol, glutathione, phenols, flavonoids, steroids, alkaloids and terpenoids (Devi and Urooj, 2008; Devi and Urooj, 2010). Gallium (Ga), a trivalent semi-metal that is chemically similar to Fe 3+, is taken up some bacteria (Bernstein, 1998). The ability of gallium to inhibit growth of intracellular bacteria by interfering with bacterial iron metabolism was established in vitro by incorporating gallium nitrate in culture media with Mycobacterium spp. (Olakanmi et al., 2000). Those investigators further demonstrated that the antimicrobial effect was due to the Gallium moiety of the Gallium nitrate, not the nitrate. The potential use of gallium as a chemoprophylactic and chemotherapeutic agent for the control of infection (Bernstein et al., 2000). The antimicrobial effects of gallium were completely abolished when media were supplemented with excess iron, providing evidence that gallium nitrate interferes with 138

2 iron dependent mechanisms that are crucial to the growth and survival of Rhodococcus equi (Boland and Meijer, 2000). 8.2 SPECIFIC AIM The purpose of this study was to examine the antibacterial activity of the crude ethanolic extracts and the isolated compounds (Lupeol and Stigmasterol) toward urinary tract infection causing pathogens using disc diffusion method. 8.3 MATERIALS AND METHODS Collection of plant material and test organisms The stem of Costus igneus used in this experiment were collected from the nursery of the Periyar Maniammai University, Vallam, Thanjavur and identified at Rapinat herbarium, St. Joseph College, Tiruchirapalli, Tamil Nadu, India. To examine the antibacterial activity of each plant extract and the isolated compounds, five strains [Escherichia coli (MTCC 25922), Enterococcus aerogenes (MTCC 29212). Pseudomonas aeruginosa (MTCC 27853), Staphylococcus aureus (MTCC 25923) and Proteus vulgaris (MTCC 7299)] were prepared as test organisms. All the strains were procured from the Microbial Type Culture and Collection (MTCC) at Chandigarh, India. Bacterial strains were cultivated at 37ºC and maintained on nutrient agar (Difco, USA) slant at for 4ºC Preparation of ethanol extracts Extraction procedure was carried out as described in chapter VI Antibacterial activity of crude ethanolic extract and the isolated compound (disc diffusion method) Antibacterial activity of crude ethanolic extract and the isolated compound were determined using the disc diffusion method (Ignacimuthu et al., 2009). The petridishes (diameter 60 mm) was prepared with Muller Hinton Agar and inoculated with test organisms. Sterile disc of six millimeter width were impregnated with 10 µl of crude ethanolic extract of Costus igneus at various concentrations of mg/ml 139

3 and for isolated compound mg/ml respectively. Prepared discs were placed onto the top layer of the agar plates and left for 30 minute at room temperature for compound diffusion. Negative control was prepared using the respective solvent and chloramphenicol (10 µg/disc) was used as a positive control. The dishes were incubated for 24 h at 37ºC and the zone of inhibition was recorded in millimeters and the experiment was repeated twice Minimum inhibitory concentration (MIC) for crude extract and isolated compound A broth dilution technique was used to determine the MIC values of crude ethanolic extract and isolated compound from the stem of Costus igneus. The crude ethanolic extract and isolated compound was dissolved in 2% dimethyl sulfoxide (DMSO) aqueous solution. A volume of 0.1 ml from each serial dilution of crude extract concentration ( µg/ml) and for isolated compound ( µg/ml) was added into tubes and were inoculated with 0.1 ml of overnight cultured bacterial strains ( 10 8 cfu/ml) and incubated at 31ºC for 48 h. Chloramphenicol was used as a positive control. The MIC value was the lowest concentration of compound that showed no growth after 48h of incubation in comparison with the control tube, which included 9.8 ml of nutrient broth and 0.1 ml bacterial strains in addition to 0.1 ml of test compound concentration (un incubated). 8.4 RESULTS ND DISCUSSIONS Antibacterial activity of crude extracts and isolated compounds The crude extract and isolated compounds from the stem of Costus igneus were tested against the UTI causing pathogenic microbes viz. Escherichia coli a most common bacteria of which virulent strains can cause gastroenteritis, urinary tract infections, neonatal meningitis; Staphylococcus aureus which cause septicemia and endocarditis in immune compromised patients; Pseudomonas aeruginosa which infects the pulmonary tract, urinary tract, burns and wounds (Nascimento et al., 2000). The results of the antimicrobial activity of crude extracts and isolated 140

4 compounds were tested against UTI by disk diffusion method are shown in (Tables 8.1 and 8.2). The crude extracts showed growth inhibitory activity against Escherichia coli and Enterococcus aerogenes (15 mm), Pseudomonas aeruginosa and Staphylococcus aureus (14 mm) at concentration 250 mg/ml (Figure 8.1). At concentration 200 mg/ml, the crude extracts exhibited the antibacterial activity all the five bacteria, but was more susceptible against Escherichia coli (14 mm), Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus aerogenes (13 mm). However, the crude extract showed better inhibitory actions against urinary tract infection causing pathogens at a concentration 150, 200 and 250 mg/ml than at lower concentration. As the concentration of extracts increased from mg/ml, the inhibitory actions of the plant extracts increased towards all the strains used in this study. Agar disc diffusion method was used to evaluate the antibacterial activity of isolated compounds by measuring the inhibition zone against the test microorganisms. Lupeol (4 mg/disc) exhibited the prominent antibacterial activity all the five bacteria but was more susceptible against Escherichia coli and Enterococcus aerogenes (22 mm) and Pseudomonas aeruginosa (20 mm) whereas for stigmasterol (4 mg/disc) was more susceptible against Enterococcus aerogenes (22 mm), Staphylococcus aureus (20 mm) and Pseudomonas aeruginosa (19 mm). At lower concentrations (0.50 mg/disc), Lupeol showed maximum inhibitory activity against Escherichia coli (15 mm), Pseudomonas aeruginosa (16 mm) followed by Staphylococcus aureus and Enterococcus aerogenes (11 mm) (Figure 8.2). whereas, Stigmasterol (0.5 mg/discs) showed activity against Enterococcus aerogenes (16 mm), Escherichia coli (14 mm), Proteus vulgaris (13 mm) (Figure 8.3). All compound concentrations were active against all tested pathogens. Among all, Lupeol exhibited more pronounced activity. The Bioactive compound isolated from stem bark of petroleum extract of Grewia titiaefolae demonstracted significant antibacterial activity against gram negative bacteria Pseudomonas aeruginosa and Klebsiella pneumonia (Mohamed Khadeer Ahamed et al., 2007). The extracts of medicinal plants and gallium nitrate 141

5 showed optimum activity against all tested urinary tract infection pathogens. However, result of disc diffusion method as indicated in (Table 8.2) revealed that gallium nitrate represent significant activity against the selected organism. Table 8.1 Antibacterial activity of ethanolic extracts of stem of Costus igneus Plant extracts Costus igneus (stems) Gallium nitrate Concentration (mg/ml) µl/disc 20 µl/disc Escherichia Coli 10± ± ± ± ± ± ± 0.81 Organisms/Zone of inhibition (mm±sd) Pseudomonas aeroginosa 8± ± ± ± ± ±0.3 22±0.21 Staphylococcus aureus 8 ± ± ± ± ± ± ±0.40 Enterococcus aerogenes 8 ± ± ± ± ± ± ± 0.21 Proteus vulgaris 7± ±0.3 11± ± ± ± ±0.21 Chloramphenicol 10 µl/disc 22± ± ± ± ± 0.81 DMSO 10 µl/disc Values showed the diameter of inhibitory zone (mm)±sd for each four replicates. The mean values are significant different at p<0.05 level. DMSO denotes dimethyl sulfoxide. Table 8.2 Antibacterial activity of isolated compound from the stem of Costus igneus Isolated Compound Concentration (mg\ml) Escherichia Coli Organisms\Zone of inhibition (mm) Pseudomonas aeroginosa Staphylococcus aureus Enterococcus aerogenes Proteus vulgaris Lupeol Stigmasterol Gallium nitrate Chloramphenicol DMSO µl/disc 10 µl/disc 10 µl/disc 21± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± Values showed the diameter of inhibitory zone (mm)±sd for each four replicates. The mean values are significant different at p<0.05 level. DMSO denotes dimethyl sulfoxide 18 ± ± ± ± ± ± ± ± ± ±

6 8.4.2 Determination of minimum inhibitory concentration (MIC) of crude extract and isolated compounds In view of the results obtained by the disc diffusion method, the minimum inhibitory concentration (MIC) of crude extract and active compounds from Costus igenus were determined by broth dilution technique (Tables 8.3 and 8.4). The highest MIC value for crude extract (25 µg/ml) was observed against Staphylococcus aureus, while Proteus vulgaris (25 µg/ml) and the lowest MIC value for crude extract (3.12 µg/ml) was observed against Escherichia coli and Pseudomonas aeruginosa (6.25 µg/ml). Lupeol and Stigmasterol showed antibacterial activity against the five urinary tract infection causing pathogens used. Lupeol was generally the most effective of the two compounds (Table 8.4). Both compounds were compared with standard antibiotic chloramphenicol. The lowest MIC of Lupeol (15.6 µg/ml) were recorded against Escherichia coli and Enterococcus aerogenes followed by Pseudomonas aeruginosa and Staphylococcus aureus, the MIC value (31.2 µg/ml). The highest MIC of Lupeol (62.5 µg/ml) was observed against Proteus vulgaris. The lowest MIC value for Stignasterol (15.6 µg/ml) were recorded against Staphylococcus aureus, whereas Pseudomonas aeruginosa and Enterococcus aerogenes exhibited the lowest MIC (31.2 µg/ml). The standard chloramphenicol was active against all reference bacteria (MIC range µg/ml; zone of inhibition range: mm). Among all Lupeol demonstracted good antibacterial activity against all the tested pathogens. Table 8.3 Minimal Inhibitory Concentration of ethanolic extract of Costus igneus Microorganisms MIC values (µg/ml) Ethanol extracts Chloramphenicol Gallium nitrate Escherichia coli Pseudomonas aeroginosa Staphylococcus aureus Enterococcus aerogenes Proteus vulgaris

7 Table 8.4 Minimal Inhibitory Concentration of isolated compound from the stem of Costus igneus Microorganisms MIC values (µg/ml) Lupeol Stigmasterol Chloramphenicol Gallium Nitrate Escherichia coli Pseudomonas aeroginosa Staphylococcus aureus Enterococcus aerogenes Proteus vulgaris CONCLUSION These results suggest that pentacyclic triterpenoid compound Lupeol and steroidal compound Stigmasterol isolated from the stem of Costus igneus had good antibacterial activity against five selected urinary tract infection causing organisms as compared with crude ethanolic Costus igneus extracts. Lupeol from Costus igneus represent a significant activity against selected urinary tract infection pathogens as compared with Stigmasterol compound. 144

8 Figure 8.1 The Antibacterial activity of crude ethanolic extract of stem of Costus igneus on the test organisms 145

9 Figure 8.2 The Antibacterial activity of Lupeol from crude ethanolic extract of stem of Costus igneus on the test organisms 146

10 Figure 8.3 The Antibacterial activity of Stigmasterol from crude ethanolic extract of stem of Costus igneus on the test organisms 147

Chapter 4. Anti-bacterial studies of PUFA extracts from Sardinella longiceps and Sardinella fimbriata. 4.1 Introduction

Chapter 4. Anti-bacterial studies of PUFA extracts from Sardinella longiceps and Sardinella fimbriata. 4.1 Introduction Anti-bacterial studies of PUFA extracts from Sardinella longiceps and Sardinella fimbriata C o n t e n t s 4.1 Introduction 4.2 Materials and Methods 4.2.1 Extract Preparation and Determination of PUFA

More information

Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn

Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn Antibacterial activities of extracts and their fractions of leaves of Tridax procumbens Linn Dhasarathan. P 1, Hemalatha. N 2, Theriappan. P 1 and Ranjitsingh, A.J.A 3 1 Department of Biotechnology, Prathyusha

More information

Higher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).

Higher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991). 4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine

More information

ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010

ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010 ANTIMICROBIAL ACTIVITY OF NON EDIBLE SEEDS AGAINST IMPORTANT PATHOGENIC MICROORGANISMS PROJECT REFERENCE NO.: 38S _B_MSC_010 COLLGE BRANCH GUIDE STUDENTS : UNIVERSITY OF MYSORE, MYSORE : DEPARTMENT OF

More information

International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN

International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil

More information

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes

Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu

More information

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp.

Chandan Prasad.et.al. Int. Journal of Engineering Research and Application ISSN : , Vol. 7, Issue 9, ( Part -6) September 2017, pp. RESEARCH ARTICLE OPEN ACCESS Antibacterial properties of Unripened Fruits of Trapa natans, Annona squamosa, Ficus carica and Anthocephalus cadamba against Pathogenic Gram Positive and Negative Bacteria.

More information

ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR

ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF TRAGIA INVOLUCRATA L. USING UV-VIS AND FTIR Sahaya Sathish S, Vijayakanth P, Palani R, Thamizharasi T and Vimala A Department of Botany, St. Joseph s College

More information

Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials

Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials ISPUB.COM The Internet Journal of Microbiology Volume 7 Number 1 Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials T Wadhwani, K Desai, D Patel, D Lawani,

More information

Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials

Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials The Internet Journal of Microbiology 2009 : Volume 7 Number 1 Effect of various solvents on bacterial growth in context of determining MIC of various antimicrobials Teena Wadhwani M.Sc.(semester IV) Nirma

More information

ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT.

ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT. International Journal of Advanced Research and Review www.ijarr.in ANTIBACTERIAL ACTIVITY OF GYMNEMA SYLVESTRE HYDROALCOHOLIC LEAF EXTRACT. Dr.Mayuri Thanwar 1, Dr.Dhananjay Dwivedi 2 1. Scientific Officer,

More information

Determination of MIC & MBC

Determination of MIC & MBC 1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight

More information

In vitro study of antibacterial activity of Carissa carandas leaf extracts

In vitro study of antibacterial activity of Carissa carandas leaf extracts Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (1):36-40 ISSN : 2249-7412 CODEN (USA): AJPSKY In vitro study of antibacterial activity of Carissa

More information

SCREENING THE BIOACTIVE POTENTIAL OF PROTEIN ISOLATED FROM CYPRINUS CARPIO. Iyyanuchamy, S.K and A. Periyanayagasamy*

SCREENING THE BIOACTIVE POTENTIAL OF PROTEIN ISOLATED FROM CYPRINUS CARPIO. Iyyanuchamy, S.K and A. Periyanayagasamy* Research Paper: Iyyanuchamy and Periyanayagasamy., 2011: Pp.6-10 SCREENING THE BIOACTIVE POTENTIAL OF PROTEIN ISOLATED FROM CYPRINUS CARPIO Iyyanuchamy, K and A. Periyanayagasamy* Department of Microbiology,

More information

Antimicrobial activity of Terminalia chebula

Antimicrobial activity of Terminalia chebula , ISSN 2249 4340 Vol. 1, No. 2, pp. 175-179, September 2011 RESEARCH ARTICLE Antimicrobial activity of Terminalia chebula M. Golam MOSTAFA, Mahdia RAHMAN, M. Manjurul KARIM* Department of Microbiology,

More information

Octa Journal of Biosciences

Octa Journal of Biosciences Octa Journal of Biosciences ISSN 2321 3663 International peer-reviewed journal June 2014 Octa. J. Biosci. Vol. 2(1):59-63 Octa Journal of Biosciences journal homepage: www.sciencebeingjournal.com Antimicrobial

More information

Evaluation of Antibacterial and Antifungal Activities of Leaf and Seed Extracts of Croton Tiglium Plant against Skin Disease Causing Microbes

Evaluation of Antibacterial and Antifungal Activities of Leaf and Seed Extracts of Croton Tiglium Plant against Skin Disease Causing Microbes International Journal of Research Studies in Biosciences (IJRSB) Volume 3, Issue 5, May 2015, PP 139-144 ISSN 2349-0357 (Print) & ISSN 2349-0365 (Online) www.arcjournals.org Evaluation of Antibacterial

More information

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography

Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Screening of Antimicrobials of some Medicinal Plants by TLC Bioautography Middha Himanshu 1* and Parihar Pradeep 2 1Department of Microbiology, DTM College of Biosciences, Bikaner, Rajasthan 2 Lovely Professional

More information

Antimicrobial activity of different extracts of leaf of Moringa oleifera (Lam) against gram positive and gram negative bacteria

Antimicrobial activity of different extracts of leaf of Moringa oleifera (Lam) against gram positive and gram negative bacteria ISSN: 2319-7706 Volume 2 Number 12 (2013) pp. 514-518 http://www.ijcmas.com Original Research Article Antimicrobial activity of different extracts of leaf of Moringa oleifera (Lam) against gram positive

More information

*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION

*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION BIOLOGIA (PAKISTAN) 01, 8 (1&), 17-17 PK ISSN 000-09 Antibacterial activity of crude s of different parts of Butea monosperma (Lamk.) Taub. *MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN Department of Botany,

More information

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:

International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: Research Article CODEN: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com SYNERGISTIC ANTIBACTERIAL ACTIVITY OF CLOVE, CUMIN

More information

Evaluation of antimicrobial activity and Bidens biternata ehrenb Leaves

Evaluation of antimicrobial activity and Bidens biternata ehrenb Leaves Human Journals Research Article October 2015 Vol.:1, Issue:4 All rights are reserved by Nadiminti Satish Reddy et al. Evaluation of antimicrobial activity and Bidens biternata ehrenb Leaves Keywords: Bidens

More information

Evaluation of Antibacterial Effect of Odor Eliminating Compounds

Evaluation of Antibacterial Effect of Odor Eliminating Compounds Evaluation of Antibacterial Effect of Odor Eliminating Compounds Yuan Zeng, Bingyu Li, Anwar Kalalah, Sang-Jin Suh, and S.S. Ditchkoff Summary Antibiotic activity of ten commercially available odor eliminating

More information

St. Joseph's Journal of Humanities and Science ISSN:

St. Joseph's Journal of Humanities and Science ISSN: M. Parimala Celia et al. / St. Joseph s Journal of Humanities and Science (Volume 4 Issue 1 January 2017) 23-28 33 St. Joseph s Journal of Humanities and Science (Volume 4 Issue 2 August 2015) 33-38 St.

More information

COMPARATIVE ANTI MICROBIAL STUDY OF SHUDDHA KASISA AND KASISA BHASMA

COMPARATIVE ANTI MICROBIAL STUDY OF SHUDDHA KASISA AND KASISA BHASMA Research Article International Ayurvedic Medical Journal ISSN:2320 5091 COMPARATIVE ANTI MICROBIAL STUDY OF SHUDDHA KASISA AND KASISA BHASMA Dr. Nisha Kumari.P. R *Dr. Dinesh Nayak J **, Dr. Sathyanarayana

More information

International Journal of Pharma and Bio Sciences A COMPARITIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SOME HERBS AND THEIR SYNERGISTIC EFFECT ABSTRACT

International Journal of Pharma and Bio Sciences A COMPARITIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SOME HERBS AND THEIR SYNERGISTIC EFFECT ABSTRACT Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 A COMPARITIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SOME HERBS AND THEIR SYNERGISTIC EFFECT SNEHA GOGTE*¹ AND

More information

Research Article. Study on antibacterial activity of some medicinally important plants

Research Article. Study on antibacterial activity of some medicinally important plants Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 205, 7():2520-252 Research Article ISSN : 0975-78 CODEN(USA) : JCPRC5 Study on antibacterial activity of some medicinally

More information

Roula M. Abdel-Massih Dept. of Biology, University of Balamand, Lebanon

Roula M. Abdel-Massih Dept. of Biology, University of Balamand, Lebanon http://skinnywithfiber.org Roula M. Abdel-Massih Dept. of Biology, University of Balamand, Lebanon Ilex paraguariensis Classification and characteristics Family Aquifoliaceae Found mainly in South America

More information

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES Int. J. Chem. Sci.: 8(1), 2010, 559-564 IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES R. MEERA *, P. DEVI a, P. MUTHUMANI, B. KAMESWARI b and B. ESWARAPRIYA c Department

More information

Phytochemical screening and antibacterial properties of Garcinia kola

Phytochemical screening and antibacterial properties of Garcinia kola 2013; 2(3): 34-38 Online at: www.phytopharmajournal.com Research Article ISSN 2230-480X JPHYTO 2013; 2(3): 34-38 2013, All rights reserved Phytochemical screening and antibacterial properties of Garcinia

More information

Jigna Parekh, Nehal Karathia and Sumitra Chanda*

Jigna Parekh, Nehal Karathia and Sumitra Chanda* African Journal of Biomedical Research, Vol. 9 (); 53-5 ISSN 1119 59 Ibadan Biomedical Communications Group Full Length Research Article Evaluation of antibacterial activity and phytochemical analysis

More information

ANTIBACTERIAL ACTIVITY OF LEAF AND SEED EXTRACTS OF DELONIX REGIA AND ACHYRANTHUS ASPERA AGAINST SELECTED BACTERIAL STRAINS

ANTIBACTERIAL ACTIVITY OF LEAF AND SEED EXTRACTS OF DELONIX REGIA AND ACHYRANTHUS ASPERA AGAINST SELECTED BACTERIAL STRAINS Int. J. Pharm. Med. & Bio. Sc. 2013 Dhanalakshmi D and Manimegalai K, 2013 Research Paper ISSN 2278 5221 www.ijpmbs.com Vol. 2, No. 2, April 2013 2013 IJPMBS. All Rights Reserved ANTIBACTERIAL ACTIVITY

More information

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com FORMULATION AND EVALUATION OF HERBAL WASH FOR NASAL HEALTH

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2010, 2(6):86-91 In vitro studies on antibacterial activity and

More information

Influence of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Cardaria draba (Lepidium draba L.) on Human Pathogenic Bacteria

Influence of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Cardaria draba (Lepidium draba L.) on Human Pathogenic Bacteria Influence of the crude Phenolic, Alkaloid and Terpenoid extracts of Cardaria draba (Lepidium draba L.) on Human Pathogenic Bacteria Nebras M. Sahi Al-Khafaji 1, Ali Hussein Al-Marzoqi 2* (Corresponding

More information

Antimicrobial Activity of Black Fruit Variant of Solanum nigrum L.

Antimicrobial Activity of Black Fruit Variant of Solanum nigrum L. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 (2017) pp. 2706-2713 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.605.303

More information

In vitro Evaluation of Antibacterial Activity of Bark and Flower Extracts of Pimenta officinalis Lindl.

In vitro Evaluation of Antibacterial Activity of Bark and Flower Extracts of Pimenta officinalis Lindl. ADVANCES IN BIORESEARCH, Vol 1 [2] December 2010: 61-68 Society of Education, India http://www.soeagra.com ISSN 0976-4585 RESEARCH PAPER In vitro Evaluation of Antibacterial Activity of Bark and Flower

More information

Research Journal of Pharmaceutical, Biological and Chemical Sciences

Research Journal of Pharmaceutical, Biological and Chemical Sciences ISSN: 09758585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Detection of Antimicrobial Activity of Oscimum sanctum (Tulsi) & Trigonella foenum graecum (Methi) against some selected

More information

Antimicrobial assessment of ethanolic extract of Costus afer Leaves

Antimicrobial assessment of ethanolic extract of Costus afer Leaves Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (3):335-341 ISSN : 2249-7412 CODEN (USA): AJPSKY Antimicrobial assessment of ethanolic extract of

More information

Antimicrobial activity of some medicinal plants against multidrug resistant skin pathogens

Antimicrobial activity of some medicinal plants against multidrug resistant skin pathogens Journal of Medicinal Plants Research Vol. 5(16), pp. 3856-3860, 18 August, 2011 Available online at http://www.academicjournals.org/jmpr ISSN 1996-0875 2011 Academic Journals Full Length Research Paper

More information

Puducherry. Antimicrobial activity, Crude drug extraction, Zone of Inhibition, Culture Media, RVSPHF567.

Puducherry. Antimicrobial activity, Crude drug extraction, Zone of Inhibition, Culture Media, RVSPHF567. ANTI-MICROBIAL ACTIVITY OF THE CRUDE DRUGS AND THE POLYHERBAL FORMULATION (RVSPHF567) BY STANDARDIZED CUP AND PLATE METHOD C.S. Kandasamy 1,2*, Suman Nath 2, P. Arulraj 1,2, V. Gopal 3, P. Muthusamy 4,

More information

Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam

Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam Human Journals Research Article October 2016 Vol.:7, Issue:3 All rights are reserved by A. Lavanya et al. Antimicrobial Potential of Whole Plant and Callus Extract of Aristolochia bracteolata Lam Keywords:

More information

Evaluation of Antibacterial Activity of Disulfiram.

Evaluation of Antibacterial Activity of Disulfiram. International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.6, No.5, pp 1476-1481, Sept-Oct 2014 Evaluation of Antibacterial Activity of Disulfiram., Muthukumar.V Janakiraman.K

More information

[PHAR06] Anti Helicobacter pylori from extracts of ten species of Phyllanthus sp. with special emphasis on chloroform extracts of Phyllanthus pulcher

[PHAR06] Anti Helicobacter pylori from extracts of ten species of Phyllanthus sp. with special emphasis on chloroform extracts of Phyllanthus pulcher The th Annual Seminar of National Science Fellowship [PHAR] Anti Helicobacter pylori from extracts of ten species of Phyllanthus sp. with special emphasis on chloroform extracts of Phyllanthus pulcher

More information

SUPPORTING INFORMATION. Studies on antimicrobial evaluation of some 1-((1-(1H-benzo[d]imidazol-2-

SUPPORTING INFORMATION. Studies on antimicrobial evaluation of some 1-((1-(1H-benzo[d]imidazol-2- SUPPORTIG IFORMATIO Studies on antimicrobial evaluation of some 1-((1-(1H-benzo[d]imidazol-2- yl)ethylidene)amino)-6-((arylidene)amino)-2-oxo-4-phenyl-1,2-dihydropyridine-3,5- dicarbonitriles C Desai*,

More information

Pelagia Research Library. A Study on Antibacterial Properties of Rosa indica against Various Pathogens

Pelagia Research Library. A Study on Antibacterial Properties of Rosa indica against Various Pathogens Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2011, 1 (1): 22-30 A Study on Antibacterial Properties of Rosa indica against Various Pathogens Jahir Alam

More information

Antibacterial Activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo Against Seven Standard Pathogenic Bacteria

Antibacterial Activity of Francoeuria crispa, Pulicaria undulata, Ziziphus spina-christi and Cucurbita pepo Against Seven Standard Pathogenic Bacteria Ethnobotanical Leaflets : 733. 09. Antibacterial Activity of,, Ziziphus spinachristi and Against Seven Standard Pathogenic Bacteria Hatil Hashim ElKamali 1* and Sana AlTayeb Mahjoub 2 1 Department of Botany,

More information

Jl. Perintis Kemerdekaan KM.5 Makassar 90231, South Sulawesi Indonesia.

Jl. Perintis Kemerdekaan KM.5 Makassar 90231, South Sulawesi Indonesia. ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com ANTIMICROBIAL POTENCY OF PASSIFLORA FOETIDA LINN FROM SOUTH SULAWESI INDONESIA AGAINST BACTERIA IN VITRO Andi

More information

Affinity of Doripenem and Comparators to Penicillin-Binding Proteins in Escherichia coli and ACCEPTED

Affinity of Doripenem and Comparators to Penicillin-Binding Proteins in Escherichia coli and ACCEPTED AAC Accepts, published online ahead of print on February 00 Antimicrob. Agents Chemother. doi:./aac.01-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights

More information

ANTIBACTERIAL EFFECTS OF CRUDE EXTRACT OF Azadirachta indica AGAINST Escherichia coli and Staphylococcus aureus

ANTIBACTERIAL EFFECTS OF CRUDE EXTRACT OF Azadirachta indica AGAINST Escherichia coli and Staphylococcus aureus International Journal of Science, Environment and Technology, Vol. 2, No 5, 2013, 989 993 ISSN 2278-3687 (O) ANTIBACTERIAL EFFECTS OF CRUDE EXTRACT OF Azadirachta indica AGAINST Escherichia coli and Staphylococcus

More information

Journal of Research in Biology

Journal of Research in Biology Journal of Research in Biology Original Research paper An International Online Open Access Publication group Assessment of the in vitro antibacterial activity of honey on some common human pathogens Journal

More information

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES OF CANAVALIA MOLLIS WIGHT & ARN. (HORSE BEAN).

ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES OF CANAVALIA MOLLIS WIGHT & ARN. (HORSE BEAN). Britto et al., IJPSR, 2011; Vol. 2(1): 95-101 ISSN: 0975-8232 IJPSR (2011), Vol. 2, Issue 1 (Research Article) Received on 14 August, 2010; received in revised form 12 November, 2010; accepted 26 December,

More information

Adenium Biotech. Management: - Peter Nordkild, MD, CEO, ex Novo Nordisk, Ferring, Egalet - Søren Neve, PhD, project director, ex Lundbeck, Novozymes

Adenium Biotech. Management: - Peter Nordkild, MD, CEO, ex Novo Nordisk, Ferring, Egalet - Søren Neve, PhD, project director, ex Lundbeck, Novozymes Adenium Biotech Management: - Peter Nordkild, MD, CEO, ex Novo Nordisk, Ferring, Egalet - Søren Neve, PhD, project director, ex Lundbeck, Novozymes Board of Directors: - Stephan Christgau, PhD, chairman,

More information

Department of Employment, Economic Development and Innovation

Department of Employment, Economic Development and Innovation Department of Employment, Economic Development and Innovation Berringa bioactive honey Comparison of honey vs silver for antibacterial activity January 2012 This project was commissioned by: Peter Woodward

More information

SCREENING OF ANTIMICROBIAL ACTIVITY OF OILS FROM DIFFERENT SPECIES OF OSCIMUM AGAINST PATHOGENIC BACTERIAL STRAINS

SCREENING OF ANTIMICROBIAL ACTIVITY OF OILS FROM DIFFERENT SPECIES OF OSCIMUM AGAINST PATHOGENIC BACTERIAL STRAINS I: 1077-1085 ISSN: 2277 4998 SCREENING OF ANTIMICROBIAL ACTIVITY OF OILS FROM DIFFERENT SPECIES OF OSCIMUM AGAINST PATHOGENIC BACTERIAL STRAINS PRAJAPATHI DN, SAROJA K* AND NAROLKAR SN Ashok & Rita Patel

More information

CHAPTER 6 EVALUATION OF SELECTED PLANT EXTRACTS FOR EVALUATION OF SELECTED PLANT EXTRACTS FOR ANTI-ACNE ACTIVITY

CHAPTER 6 EVALUATION OF SELECTED PLANT EXTRACTS FOR EVALUATION OF SELECTED PLANT EXTRACTS FOR ANTI-ACNE ACTIVITY CHAPTER 6 EVALUATION OF SELECTED PLANT EXTRACTS FOR School of Science, SVKM s NMIMS University Page 119 6. EVALUATION OF SELECTED PLANT EXTRACTS FOR 6.1 MATERIALS AND METHODS 6.1.1 Antimicrobial assays

More information

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE RESEARCH ARTICLE INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH AND BIO-SCIENCE A Path for Horizing Your Innovative Work INVESTIGATION OF ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF CATHARANTHUS

More information

NSave Nature to Survive

NSave Nature to Survive ISSN: 0973-7049 NSave Nature to Survive : Special issue, Vol. 1; 89-94; 2010 EVALUATION OF ANTIBACTERIAL POTENTIAL OF STEM BARK OF MORINGA OLEIFERA LAM Renu Sarin et al. Moringa oleifera Antimicrobial

More information

Prof. Dr. K. Aruna Lakshmi (DEAN Academic Affairs) Dept. of Microbiology GITAM University Visakhapatnam. Under the Guidance of.

Prof. Dr. K. Aruna Lakshmi (DEAN Academic Affairs) Dept. of Microbiology GITAM University Visakhapatnam. Under the Guidance of. Antimicrobial Activity of Essential oils from Selected Culinary herbs of Zingiberaceae and Detection of Antimicrobial Compounds using TLC- Bioautography Under the Guidance of Prof. Dr. K. Aruna Lakshmi

More information

320 MBIO Microbial Diagnosis. Aljawharah F. Alabbad Noorah A. Alkubaisi 2017

320 MBIO Microbial Diagnosis. Aljawharah F. Alabbad Noorah A. Alkubaisi 2017 320 MBIO Microbial Diagnosis Aljawharah F. Alabbad Noorah A. Alkubaisi 2017 Pathogens of the Urinary tract The urinary system is composed of organs that regulate the chemical composition and volume of

More information

Comparative Evaluation on the Antibacterial Activity of Karpoori Variety Piper betle Leaves against Certain Bacterial Pathogens

Comparative Evaluation on the Antibacterial Activity of Karpoori Variety Piper betle Leaves against Certain Bacterial Pathogens Human Journals Research Article May 2016 Vol.:3, Issue:3 All rights are reserved by C. Uma et al. Comparative Evaluation on the Antibacterial Activity of Karpoori Variety Piper betle Leaves against Certain

More information

Evaluation of antibacterial activity of Abutilon theophrasti medic

Evaluation of antibacterial activity of Abutilon theophrasti medic Evaluation of antibacterial activity of Abutilon theophrasti medic Musheerul Hassan 1, Reyaz Ahmad Mir 2, Huma Habib 3* 1 Department of Life sciences, Pacific University, Udaipur, (India) 2 Department

More information

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.)

In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) INDIAN JOURNAL OF PHARMACEUTICAL & BIOLOGICAL RESEARCH (IJPBR). Vol. 1 (1), March., 13 In vitro antimicrobial activity of leaves and bark extracts of Ficus religiosa (Linn.) G.Ramakrishnaiah * 1, T. Hariprasad

More information

Life Science Archives (LSA)

Life Science Archives (LSA) Available online at www.jpsscientificpublications.com Life Science Archives (LSA) ISSN: 2454-1354 Volume 2; Issue - 2; Year 2016; Page: 514-518 Research Article ROLE OF Piper nigrum L. IN THE HEALTH CARE

More information

Myanmar Health Sciences Research Journal, Vol. 30, No. 2, Antimicrobial Activity of Justicia adhatoda L. Leaf Extracts (မ ယ က )

Myanmar Health Sciences Research Journal, Vol. 30, No. 2, Antimicrobial Activity of Justicia adhatoda L. Leaf Extracts (မ ယ က ) Myanmar Health Sciences Research Journal, Vol. 30, No. 2, 2018 Antimicrobial Activity of Justicia adhatoda L. Leaf Extracts (မ ယ က ) Khin May Thi 1*, Saw Myat Thwe 1, Soe Myint Aye 2, Aye Thida Htun 1,

More information

A study on phytochemical analysis and antimicrobial activity of Hyptis suaveolens (L.) poit

A study on phytochemical analysis and antimicrobial activity of Hyptis suaveolens (L.) poit Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(6):438-442 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A study on phytochemical analysis and antimicrobial

More information

Antimicrobial and Phytochemical Analysis of Centella

Antimicrobial and Phytochemical Analysis of Centella Antimicrobial and Phytochemical Analysis of Centella asiatica (L.) R. Perumal Samy*,1, Ignacimuthu 2, Vincent TK Chow 1 1 Infectious Diseases Programme, Department of Microbiology, Department of Anatomy,

More information

Evaluation of antibacterial potential of some Indian honey samples against throat and skin infective pathogens

Evaluation of antibacterial potential of some Indian honey samples against throat and skin infective pathogens ISSN: 2319-7706 Volume 3 Number 1 (2014) pp. 362-369 http://www.ijcmas.com Original Research Article Evaluation of antibacterial potential of some Indian honey samples against throat and skin infective

More information

Research Article. Antibacterial activity of Sivanar Vembu (Indigofera aspalathoides) against some human pathogenic bacteria

Research Article. Antibacterial activity of Sivanar Vembu (Indigofera aspalathoides) against some human pathogenic bacteria Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(3):937-941 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Antibacterial activity of Sivanar Vembu (Indigofera

More information

EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME EXTRACT

EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME EXTRACT International Journal of Allied Practice, Research and Review Website: www.ijaprr.com (ISSN 2350-1294) EVALUATION OF ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF Zingiber officinale (GINGER) RHIZOME

More information

Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon combination with bacteriocin

Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon combination with bacteriocin ISSN: 2319-7706 Volume 3 Number 9 (2014) pp. 617-621 http://www.ijcmas.com Original Research Article Enhanced antibacterial potential of ethanolic extracts of neem leaf (Azadiracta indica A. Juss.) upon

More information

Antioxidant and Antimicrobial Activities of the Extracts of Sophora flavescens

Antioxidant and Antimicrobial Activities of the Extracts of Sophora flavescens Antioxidant and Antimicrobial Activities of the Extracts of Sophora flavescens Cheng-Hong Yang, Chi-Chun Wu, and Li-Yeh Chuang * Abstract The main goal of this study was to determine the antibacterial

More information

International Journal of Applied And Pure Science and Agriculture

International Journal of Applied And Pure Science and Agriculture Scientific Journal Impact Factor: 3.76 e- ISSN: -553 p- ISSN: -83X International Journal of Applied And Pure Science and Agriculture www.ijapsa.com ROLE OF MICROBIAL BEHAVIOR IN EXTRACT OF CLEOME GYNANDRA

More information

Isolation of Herbal Plants: Antifungal and Antibacterial Activities

Isolation of Herbal Plants: Antifungal and Antibacterial Activities Isolation of Herbal Plants: Antifungal and Antibacterial Activities Rathi Sanjesh G 1*, Patel Kanu R 2, Bhaskar Vaidhun H 3 1. Shri JagdishPrasad Jhabarmal Tibrewala University, Vidyanagari, Jhunjhunu,

More information

Phytochemical analysis and antibacterial properties of leaf extract of Azima tetracantha (Lam.)

Phytochemical analysis and antibacterial properties of leaf extract of Azima tetracantha (Lam.) Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (2):110-114 ISSN : 2249-7412 CODEN (USA): AJPSKY Phytochemical analysis and antibacterial properties

More information

In-House Standardization of Carba NP Test for Carbapenemase Detection in Gram Negative Bacteria

In-House Standardization of Carba NP Test for Carbapenemase Detection in Gram Negative Bacteria International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.701.342

More information

In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method

In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method Tropical Biomedicine 22(2): 165 170 (2005) In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method Zaidan, M.R.S. 1, Noor Rain, A. 1, Badrul, A.R. 2, Adlin,

More information

Antimicrobial activity of Karuveppilai vadagam against Enteric pathogens

Antimicrobial activity of Karuveppilai vadagam against Enteric pathogens International Journal of Current Research in Medical Sciences ISSN: 2454-5716 P-ISJN: A4372-3064, E -ISJN: A4372-3061 www.ijcrims.com Original Research Article Volume 4, Issue 6-2018 Antimicrobial activity

More information

Leaf Extract. Abs. Joseph M. *Corresponding. author: organisims: Plant Material. tract. Tamilnadu, India. several herbal. India.

Leaf Extract. Abs. Joseph M. *Corresponding. author: organisims: Plant Material. tract. Tamilnadu, India. several herbal. India. International Journal of Phytomedicine 4 (2012) 456460 http://www.arjournals.org/index.php/ijpm/index Original Research Article ISSN: 09750185 Phytochemical and Antimicrobial Studies of Phyllanthus Wightianus

More information

Phytochemical study and bioactivity of solvent extracts on Coriandrum sativum

Phytochemical study and bioactivity of solvent extracts on Coriandrum sativum International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com Volume 3, Issue 5-2016 Research Article Phytochemical study and bioactivity of solvent extracts on Coriandrum

More information

Mohammed Al-janabi and ZainabYaseen

Mohammed Al-janabi and ZainabYaseen Pak. J. Biotechnol. Vol 15 (3) 843-848 (2018) ISSN Print: 18-1837 www.pjbt.org ISSN Online: 23-7791 ANIBACIAL ACIVIY OF CLOVS SDS OF Syzygium aromaticum and Cinnamon Cinnamomum verum BAK SSNIAL OILS Mohammed

More information

International Journal of Advanced Research in Biological Sciences ISSN: Research Article

International Journal of Advanced Research in Biological Sciences ISSN: Research Article International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com Research Article Antibacterial activity of Mangrove Medicinal Plants against Gram positive Bacterial pathogens

More information

Screening of Antibacterial Activity of the Medicinal Plant Phyllanthus amarus Against Urinary Tract Infection Causing Bacterial Pathogens

Screening of Antibacterial Activity of the Medicinal Plant Phyllanthus amarus Against Urinary Tract Infection Causing Bacterial Pathogens Applied Journal of Hygiene 1 (3): 19-24, 2012 ISSN 2309-8910 IDOSI Publications, 2012 DOI: 10.5829/idosi.ajh.2012.1.3.71111 Screening of Antibacterial Activity of the Medicinal Plant Phyllanthus amarus

More information

Screening for Antimicrobial Activity in Acanthus ilicifolius

Screening for Antimicrobial Activity in Acanthus ilicifolius Available online at www.scholarsresearchlibrary.com Scholars Research Library Archives of Applied Science Research, 2010, 2 (5):311-315 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-508X

More information

Mr. Vipul R. Suryavanshi Department of Pharmaceutical Analysis Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai

Mr. Vipul R. Suryavanshi Department of Pharmaceutical Analysis Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai Resazurin microtitre assay (REMA) for antibacterial and antifungal activity of herbs of three antidiarrhoeal formulations: Bilagyl and Berbenterone tablets and Berbenterone suspension Mr. Vipul R. Suryavanshi

More information

ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF SERIAL EXTRACTS FROM LEAVES OF AEGLE MARMELOS (LINN.)

ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF SERIAL EXTRACTS FROM LEAVES OF AEGLE MARMELOS (LINN.) Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 68 No. 5 pp. 687ñ692, 2011 ISSN 0001-6837 Polish Pharmaceutical Society NATURAL DRUGS ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF SERIAL EXTRACTS

More information

INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES

INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE SCIENCES International Standard Serial Number (ISSN): 2249-6807 International Journal of Institutional Pharmacy and Life Sciences 5(4): July-August 2015 INTERNATIONAL JOURNAL OF INSTITUTIONAL PHARMACY AND LIFE

More information

A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum

A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum RESEARCH ARTICLE A Study on Phytochemicals, Antioxidant, Antidiabetic and Antimicrobial Activity of the Leaves of Solanum Trilobatum Fabiola 1, Dr. V. Judia Harriet Sumathy 2 PG & Research Department of

More information

ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS

ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS ANTI-MICROBIAL ACTIVITIES OF BETEL NUT (ARECA CATECHU LINN) SEED EXTRACTS Ajima Karphrom 1, Suthisak Suknaisilp 2, Pasawadee Pradeepasaena 2, Sumate Tantratian 2 * ABSTRACT Seeds of betel nuts grown in

More information

Antimicrobial activity of natural dyes obtained from Terminalia arjuna (Roxb.) Wight & Arn barks

Antimicrobial activity of natural dyes obtained from Terminalia arjuna (Roxb.) Wight & Arn barks Available online at www.worldscientificnews.com WSN 98 (2018) 221-227 EISSN 2392-2192 SHORT COMMUNICATION Antimicrobial activity of natural dyes obtained from Terminalia arjuna (Roxb.) Wight & Arn barks

More information

Screening Of Phytochemical And Antibacterial Activity Of Polygala javana Plant Leaf, Stem And Root Extract Against Human Pathogen

Screening Of Phytochemical And Antibacterial Activity Of Polygala javana Plant Leaf, Stem And Root Extract Against Human Pathogen International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.4, No.4, pp 1792-1796, Oct-Dec 2012 Screening Of Phytochemical And Antibacterial Activity Of Polygala javana Plant Leaf,

More information

Antibacterial activity of Polyalthia longifolia var. angustifolia stem bark extract

Antibacterial activity of Polyalthia longifolia var. angustifolia stem bark extract International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol. 3, No.1, pp 256-260, Jan-Mar 2011 Antibacterial activity of Polyalthia longifolia var. angustifolia stem bark extract

More information

The medicinal use of honey has been known since ancient

The medicinal use of honey has been known since ancient THE JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE Volume 13, Number 4, 2007, pp. 439 441 Mary Ann Liebert, Inc. DOI: 10.1089/acm.2007.6366 Bactericidal Activity of Different Types of Honey Against

More information

Available online at

Available online at Available online at www.ijpab.com ISSN: 2320 7051 Int. J. Pure App. Biosci. 3 (1): 203-206 (2015) INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE Research Article Antifungal Activity of the Plant Extracts

More information

Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples

Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples Background The VACUETTE Urine CCM tube is for the collection, transport and storage of urine samples

More information

Journal of PHARMACY AND BIORESOURCES

Journal of PHARMACY AND BIORESOURCES Vol. 2 no. 2, pp 131-136 (September 20(5) http.z/www.ajol.info/joumals/jpb Journal of PHARMACY AND BIORESOURCES Antibacterial activity of the stem bark of Boswellia dalzielii Moses A. Olukemi'", Yvonne

More information

Pharmacologyonline 3: (2009) Newsletter Maridass and Raju. Investigation of Phytochemical and Antimicrobial Activity of Huberzia Species

Pharmacologyonline 3: (2009) Newsletter Maridass and Raju. Investigation of Phytochemical and Antimicrobial Activity of Huberzia Species Investigation of Phytochemical and Antimicrobial Activity of Huberzia Species M. Maridass and G. Raju Department of Advanced Zoology and Biotechnology PioneerKumaraswamy College, Nagercoil-629003, Tamil

More information

Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria

Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria Screening of Antibacterial Activity of Aqueous Bark Extract of Bombax ceiba against some Gram Positive and Gram Negative Bacteria Original Article Digge V.G 1, Kuthar Sonali S 2, Hogade Maheshwar G 3,

More information

Research Article. Phytochemical and antibacterial efficacy of Hevea brasiliensis

Research Article. Phytochemical and antibacterial efficacy of Hevea brasiliensis Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(12):777-783 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Phytochemical and antibacterial efficacy of Hevea

More information

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT

PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA SEED EXTRACT INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND INVITRO ANTIBACTERIAL ACTIVITY OF CUCURBITA MAXIMA

More information