Simultaneous Determination of Seven Active Components in Mian anning Mixture by HPLC Under Multiple UV Wavelengths

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1 measurement for the determination of ribavirin by HPLC [J]. Chin J Pharm Anal(), 2007, 27(11): [9] LIU Y L, LI D M, FEN L, et al. Uncertainty of measurement in the HPLC determination of Fufangdanshen tablets [J]. Chin J Pharm Anal(), 2007, 27(11): [10] QIN L, CHEN S H, FAN Y G. Evaluation of for the content determination of vitamin B12 injection by UV [J]. J Yunnan Univ Tradit Chin Med(), 2013, 36(6): [11] QIN L, XIAO Y, CHEN S H, et al. Uncertainty evaluation for determination of pentoxyverine citrate tablets by HPLC [J]. China Pharm(), 2014, 17(3): [12] QUINTELA M, BÁGUENA J, GOTOR G, et al. Estimation of the uncertainty associated with the results based on the validation of chromatographic analysis procedures: Application to the determination of chlorides by high performance liquid chromatography and of fatty acids by high resolution gas chromatography [J]. J Chromatogr A, 2012(1223): [13] KONIECZKA P, NAMIE SNIK J. Estimating uncertainty in analytical procedures based on chromatographic techniques [J]. J Chromatogr A, 2010(1217): [14] JJG [S]. 2006: HPLC ( ) 5-() B A 7 Hypersil C 18 (4.6 mm 250 mm 5 µm) 0.7 ml min 1 A -(1 3) B 0.1%5-284 nm () 320 nm 220 nm B A 270 nm 210 nm 25 5-() B A 10.08~ µg ml 1 (r= ) 3.55~35.50 µg ml 1 (r= ) 5.80~58.00 µg ml 1 (r= ) 4.20~42.00 µg ml 1 (r= ) 40.50~ µg ml 1 (r=1.000) 12.28~ µg ml 1 (r= ) 7.90~79.00 µg ml 1 (r= ) 5-() B A 98.56% 96.94% 99.61% 97.54% % 98.59% 99.81% RSD 0.97% 1.73% 1.03% 1.35% 0.86% 1.70% 1.43%(n=6) 5-() B A R B (2015) DOI: /j.cnki.issn Simultaneous Determination of Seven Active Components in Mian anning Mixture by HPLC Under Multiple UV Wavelengths ZHANG Xiaojing 1, WANG Yingying 2, LI Jun 2 (1.Zhejiang Wenling Food and Drug Inspection Center, Wenling , China; 2.Zhejiang Taizhou Institute for Food and Drug Control, Taizhou , China) ABSTRACT: OBJECTIVE To develop an HPLC method with multiple wavelength ultraviolet for the simultaneous determination the content of 5-hydroxymethyl-2-furaldehyde, polygalaxanthone, atractylenolide, atractylenolide, salvianolic acid B, tanshinone A and catalpol in Mian anning mixture. METHODS The sample was separated on a Hypersil C 18 column(4.6 mm 250 mm, 5 µm) with a mobile phase consisting of methanol-acetonitrile(1 3) and 0.1% phosphoric acid solution. The flow rate was 0.7 ml min 1. The detection wavelength was set at 284 nm for 5-hydroxymethyl-2-furaldehyde, 320 nm for polygalaxanthone, 220 nm for atractylenolide and atractylenolide, 270 nm for salvianolic acid B and tanshinone A, 210 nm for catalpol. The column temperature was 25. RESULTS The calibration curves of 5-hydroxymethyl-2-furaldehyde, polygalaxanthone, atractylenolide, atractylenolide, salvianolic acid B, tanshinone A and catalpol were linear in the range of µg ml 1 (r= ), µg ml 1 (r= ), µg ml 1 Tel: (0576) zhangxiaojinglw@163.com 194 Chin J Mod Appl Pharm, 2015 February, Vol.32 No

2 (r= ), µg ml 1 (r= ), µg ml 1 (r=1.000), µg ml 1 (r= ), µg ml 1 (r= ), and the average recoveries(n=6) were 98.56%(RSD=0.97%), 96.94%(RSD=1.73%), 99.61% (RSD=1.03%), 97.54%(RSD=1.35%), %(RSD=0.86%), 98.59%(RSD=1.70%), 99.81%(RSD=1.43%), respectively. CONCLUSION The method is feasible, accurate and stable, which can control the quatity of Mian anning Mixture. KEY WORDS: Mian anning mixture; 5-hydroxymethyl-2-furaldehyde; polygalaxanthone ; atractylenolide ; atractylenolide ; salvianolic acid B; tanshinone A; catalpol ( ) () 7 [1-2] HPLC 5- ( )() ( ) B A 5-() B A Agilent 1200 G1311A G1330B G1322A Chemstation G1315B () 5-( %) () ( %) B( %) A( % 12 h ) ( % 12 h ) ( %)( %) (120 ml )( ) ( ) 1.2 Hypersil C 18 (4.6 mm 250 mm 5 µm) A -(1 3) B 0.1% [2-4] 0~12 min 45.0%A 12~18 min 45.0% 52.0%A 18~45 min 52.0% 70.0%A 45~69 min 70.0%A 69~80 min 70.0% 45.0%A [3-5] 0~18 min λ 1 =284 nm [6] 18~26 min λ 2 =320 nm [2] 26~39 min λ 3 =220 nm [7-9] 39~58 min λ 4 =270 nm [10] 58~80 min λ 5 =210 nm [11-12] 0.7 ml min µl ( ) <3 500 > ( ) B A ml 5-() B A ml 50 ml 5-() B A ml 50 ml Chin J Mod Appl Pharm, 2015 February, Vol.32 No.2 195

3 1.5 ( ) () µl () B A 5- () B A 1 1 A B C D E F () B 6 A 7 Fig. 1 HPLC chromatograms A reference substance; B sample solution; C sample without Rehmanniae Radix Praeparata; D sample without Polygalae Radix; E sample without Atractylodis Macrocephalae Rhizoma; F sample without Salviae Miltiorrhizae Radix et Rhizoma; 1 5-hydroxymethyl-2-furaldehyde; 2 polygalaxanthone ; 3 atractylenolide ; 4 atractylenolide I; 5 salvianolic acid B; 6 tanshinone IIA; 7 catalpol ml 5 10 ml 1.2 (Y) (X) 5- () B A Chin J Mod Appl Pharm, 2015 February, Vol.32 No

4 1 7 Tab. 1 Regression equation, correlation coefficient and linearity range of the seven components / µg ml 1 r ~ Y= X () 3.55~35.50 Y= X ~58.00 Y= X ~42.00 Y= X B 40.50~ Y= X A 12.28~ Y= X ~79.00 Y= X µl 6 7 (RSD) 5- () B A RSD 0.86% 1.13% 1.64% 0.59% 0.37% 1.02% 1.23% RSD 5-() B A RSD 0.97% 1.43% 1.57% 0.84% 0.76% 1.16% 1.29% h 20 µl 5- () B A RSD 1.02% 1.24% 1.39% 0.64% 0.59% 1.11% 0.93% 12 h ml 10 ml ml (n=9) Tab. 2 Analytical results of recoveries(n=9) 5- () B A / /mg /% mg / % RSD/ % Chin J Mod Appl Pharm, 2015 February, Vol.32 No.2 197

5 () B A 3 3 (n=3, x ± s ) Tab. 3 Results of content determination(n=3, x ± s ) 5- / ()/ / / B/ A/ / ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± % -0.1% -0.1% () -0.1% 5- -(1 3) 0.1% >2 7 REFERENCES [1] [S]. 1998: 166. [2]. [S]. 2010: 70-71, 95-96, , , 30, 36. [3]. B A [J]., 2007, 26(10): [4],,,. [J]., 2010, 29(5): [5] CAI Y Y, SHI T T, HUANG Q L. Determination of 5-hydroxymethylfurfural in Liuweidihuang pills by HPLC [J]. [J]. Chin J Mod Appl Pharm(), 2012, 29(04): [6] QIN X Y, ZHOU J, LI X Y, et al. RP-HPLC determination of 5-hydroxymethylfurfural in prepared rehmannia root [J]. J Fourth Mil Med Univ(), 2006, 27(06): [7] LIU Y Q, CAI Q. HPLC determination of atractylenolide and atractylenolide in 50 batches crude drugs and slices of Atractylodes macrocephala Koidz. from different sources [J]. Chin J Pharm Anal(), 2012, 32(7): [8] CHEN H P, ZHANG J H, WANG X Y, et al. Contents comparision of atractylode I,, in Rhizoma Atractylodis Macrocephalae and the processed with soils [J]. J Chin Med Mater( ), 2011, 34(03): [9] DUAN Q, LI C P, ZHAO Z D, et al. Quantitative determination of atractylenolides I,, in simple parching Atractylodes macrocephala by HPLC [J]. Cent South Pharm(), 2009, 7(5): [10] YU Y M, MOU N, ZHANG P, et al. Determination of cryptotanshinone and tanshinone A in Shenqi Shengji granule by HPLC [J]. [J]. Chin J Mod Appl Pharm( ), 2013, 301(4): [11],,,. HPLC [J]., 2010, 16(1): [12] MEN J Y, DONG Z M, CUI Y Q, et al. RP-HPLC simultaneous determination of catalpol and gastrodin in Tianma Toufengling capsules [J]. Chin J Pharm Anal( ), 2009, 29(6): Chin J Mod Appl Pharm, 2015 February, Vol.32 No

Rb 1. Results of variance analysis REFERENCES Chin J Mod Appl Pharm, 2014 September, Vol.31 No

Rb 1. Results of variance analysis REFERENCES Chin J Mod Appl Pharm, 2014 September, Vol.31 No 5 Tab. 5 Results of variance analysis F A 0.589 2 0.295 22.9 B 0.065 0 2 0.032 5 2.5 C 0.286 2 0.143 11.14 D( ) 0.025 8 2 0.012 9 F [4] F 0.05(2,2) =19 F 0.01(2,2) =99 Note: Chek the table of F values