Dr.Rajeshwari Basavanna, Dr.Biju George, Dr.Dolly Daniel Transfusion Medicine and Immunohaematology Department of Haematology CMC Vellore
|
|
- Alan Flowers
- 5 years ago
- Views:
Transcription
1 Dr.Rajeshwari Basavanna, Dr.Biju George, Dr.Dolly Daniel Transfusion Medicine and Immunohaematology Department of Haematology CMC Vellore
2 Background Clinical Severity of disease Autoimmune Haemolytic anaemia - decreased red cell survival / accelerated destruction, secondary to autoantibodies. Wide spectrum - Mild to fulminant life threatening anaemia. Immunoglobulin class Subclass Titer Complement activation Thermal amplitude and Functional ability of the RE system
3 Objectives To serologically characterize the type of autoantibodies resulting in AIHA and correlate with in vivo haemolysis To study the correlation between the strength of Direct Antiglobulin Test (DAT) and the severity of in vivo haemolysis.
4 Study was done over a period of 13 months. (May 2014 June 2015) Inclusion criteria Patients, with positive poly specific DAT and also noted to have haemolysis based on clinical and laboratory criteria Exclusion criteria Patients had haemopoietic stem cell transplant Neonates with HDFN
5 Classification of severity of haemolysis Laboratory parameters 1) Haemoglobin <9gm/dl 2) Bilirubin >2mg/dl 3) Reticulocyte >2% 4) LDH >500IU/ml Severe haemolysis - if all the above parameters were fulfilled, Moderate haemolysis - 2 / 3 of the above laboratory parameters mentioned above are abnormal 1Das SS, Nityanand S, Chaudhary R. Clinical and serological characterization of autoimmune hemolytic anemia in a tertiary care hospital in North India. Ann Hematol Aug;88(8): Wheeler CA, Calhoun L, Blackall DP. Warm reactive autoantibodies: clinical and serologic correlations. Am J Clin Pathol Nov;122(5):680 5.
6 Algorithm to demonstrate the methodology of the study DAT positive patients with clinical and laboratory evidence of haemolysis were enrolled for the study Based on laboratory parameters, haemolysis was categorized into severe or moderate Monospecific DAT done to identify the presence of IgM,IgA,C3d and C3c by CAT If IgG was positive, IgG subtyping was done using anti IgG1 and IgG3 by CAT
7 STATISTICAL ANALYSIS Median, mean, Standard deviation, and range was calculated for all continuous variables. Pearson and Fisher s exact chi square was used for comparison of categorical data. Mann-Whitney test is used to calculate p value of LDH, bilirubin and reticulocytes. Binary logistic regression analysis was used for comparing the categorical data and to calculate the odds ratio with 95% confidence interval (CI). P value <0.05 was considered significant.
8 Percentage Percentage Results Age distribution of patients in Primary and Secondary AIHA Gender distribution in Primary and Secondary AIHA <20 years years >40years Primary AIHA Secondary AIHA Primary Secondary Male Female
9 Percentage Patients with Severe and Moderate haemolysis (55.3%) 50 42(44.7%) Severe Moderate
10 Type of AIHA Causes of secondary AIHA Out of 94 AIHA patients, Numbers Percentage Primary AIHA- 51 (54.3%) patients Secondary AIHA - 43(45.7%) patients Autoimmune disease Lymphoproliferative disorder Infection Other causes Total
11 Correlation of severity of haemolysis with 1. Type of AIHA 2. Antibody profile identified on monospecific DAT 3. IgG subtypes 4. Strength of DAT
12 Severity of haemolysis and type AIHA 52 patients with severe haemolysis Primary AIHA - 37(71.2% ) Secondary AIHA -15(28.8% ) The association of primary AIHA with severe haemolysis was statistically significant with p< (OR = 4.76, 95% CI )
13 Distribution of autoantibodies Immunoglobulin type IgG Only Number(%) 27,(28.7%) IgG + C3d 29(30.9%) C3d alone 8.5%(8) IgG with combination 62.8%(59) IgG alone 28.7%(27) IgG + IgM + C3d 15 (16.0%) IgG + IgM + C3c + C3d 8( 8.5%) IgG + IgM 1(1.1%) IgG + IgA + C3d IgG + IgA + IgM+C3d IgG + IgA 2 (2.1%) 2 (2.1%) 1, (1.1%) IgG + IgA + IgM 1(1.1%) C3d only 8 (8.5%)
14 Distribution of solitary IgG vs IgG in combination with other autoantibodies IgG in combination Solitary IgG Odds ratio 95% CI P value N= 59 N=27 Severe haemolysis 41(89.1%) 5(10.9%) Moderate haemolysis 18 (45.0%) 22(55.0%) <0.001
15 Percentage Distribution of IgG subtypes in patient s population % % % 10 0 IgG1 Only IgG1 and / IgG3 Neither IgG1 nor IgG3
16 Correlation of Ig G subtypes with severity of haemolysis IgG Subtypes Moderate haemolysis Severe haemolysis Total number Odds ratio 95% CI p Value IgG1 19(47.5%) 31(67.4%) 50(58.1%) IgG1with IgG3 Absence of IgG1 and IgG3 3(7.5%) 7(15.2%) 10(11.6%) (45.0%) 8(17.4%) 26(30.2%) Total 40(100%) 46(100%) 86(100%)
17 IgG Positive 86 Solitarty IgG 27 (31.3%) IgG in combination with other autoantibodies 59 (68.6%) IgG1 alone 9 IgG1 with IgG3 1 Neither IgG1 nor IgG3 17 IgG1 with other auto antibodies 41 IgG1with IgG3 and other auto antibodies 9 Neither IgG1 nor IgG3 with other auto antibodies 9 Distribution of IgG and their subtypes
18 Impact of IgG subtypes in patient s with solitary IgG Moderate haemolysis Severe haemolysis Total p value IgG1 alone 4 (44.4%) 5(55.6%) 9 IgG1 with IgG3 1(100%) 0 1 Absence of IgG1 and IgG3 17(100%) 0 17 < Total 22(81.5%) 5(18.5%) 27
19 Clinical impact of IgG subtypes in patients with combination of autoantibody. Moderate haemolysis Severe haemolysis Total p value IgG1 15(36.6%) 26(63.4%) 41(100%) IgG1 with IgG3 2(22.2%) 7(77.8%) 9(100%) Absence of IgG1 and IgG3 1(11.1%) 8(88.9%) 9(100%)
20 Impact of complement fixation and IgG subtypes on severity of haemolysis Moderate haemolysis Severe haemolysis Total p value Patients with IgG1 and/or IgG3 with complement fixation 16(33.3%) 32(66.6%) 48(100%) Patients with neither IgG1 nor IgG3 with complement fixation 1(12.5%) 7(87.5%) 8(100%)
21 Impact of complement fixation in patients with IgG1 and/or IgG3 subtype Moderate haemolysis Severe haemolysis Total p value Patients with IgG1 and /or IgG3 without complement fixation 5(50%) 5(50%) 10 IgG1 and /or IgG3 with complement fixation 16(32.6%) 33(67.3%)
22 Clinical impact of complement fixation in patients with absence of IgG1 nor IgG3 Moderate haemolysis Severe haemolysis Total p value Patients with neither IgG1 nor IgG3 Patients who fixed complement with neither IgG1 nor IgG3 17(100%) (12.5%) 7(87.5%) 8 <0.001
23 Percentage Strength of Polyspecific DAT with severity of haemolysis Strength of polyspecific DAT Severe Moderate
24 Discussion The factors, which had the greatest impact on severity of haemolysis are Diagnosis of primary AIHA Presence of antibodies with complement fixation Presence of IgG1 and IgG3 subtypes Strength of DAT.
25 CONCLUSION Recommended algorithm Presence of IgG1 and IgG3 subtypes Polyspecific DAT Strength of DAT Severe haemolysi s complement fixation Diagnosis of primary AIHA Monospecific DAT In presence of IgG, and in the absence of complement fixation IgG subtyping is recommended
26 Variability is the law of life, and as no two faces are the same, so no two bodies are alike, and no two individuals react alike and behave alike under the abnormal conditions which we know as disease. William Osler
27 Thank you
AIHA The Laboratory Perspective on Testing. Tom Bullock Joint UK NEQAS (BTLP) & BBTS BBT SIG Annual Meeting 20 th November 2018
AIHA The Laboratory Perspective on Testing Tom Bullock Joint UK NEQAS (BTLP) & BBTS BBT SIG Annual Meeting 20 th November 2018 Auto Immune Haemolytic Anaemia (AIHA) BSH guideline (Hill et al. 2017): AIHA
More informationCase Report A Case of WarmAutoimmune HaemolyticAnaemiaPresenting with Intravascular Haemolysis
IBIMA Publishing International Journal of Case Reports in Medicine http://www.ibimapublishing.com/journals/ijcrm/ijcrm.html Vol. 2014 (2014), Article ID 165588, 4 pages DOI: 10.5171/2014.165588 Case Report
More informationDr. Joyce Regi M.D (Path),D.P.B. Holy Family Hospital & Research Centre
Dr. Joyce Regi M.D (Path),D.P.B. Holy Family Hospital & Research Centre Autoimmune haemolytic anaemia is charaterised by shortened red cell survival & the presence of antibodies directed against autologous
More informationBrrrr, It s Cold In Here
Brrrr, It s Cold In Here Kate Grogan, MD Transfusion Medicine Fellow Physician, BloodworksNW 4/24/15 Patient 20 year old female Viral illness Presents one week later with severe hemolysis Hgb 5.6 Increased
More informationTransfusion Guidelines in AIHA; Indications, Compatibility Testing and Administration.
Transfusion Guidelines in AIHA; Indications, Compatibility Testing and Administration. Lawrence D. Petz, M.D. Emeritus Professor University of California Los Angeles, California, U.S.A.; Medical Director
More informationWarm Reactive Autoantibodies Clinical and Serologic Correlations
Coagulation and Transfusion Medicine / CHARACTERISTICS OF WARM AUTOANTIBODIES Warm Reactive Autoantibodies Clinical and Serologic Correlations Christine A. Wheeler, MD, * Loni Calhoun, MT(ASCP)SBB, and
More informationNew phase 2 Clinical Trial Enrolling Now
New phase 2 Clinical Trial Enrolling Now Warm Autoimmune Hemolytic Anemia (waiha) and Cold Agglutinin Disease (CAD) Designed for patients Age 18 or older With a primary diagnosis of waiha who had a recurrence,
More informationCase Report. Autoimmunization in Thalassemia: A Case Report with Review of Literature
Case Report Autoimmunization in Thalassemia: A Case Report with Review of Literature Sangeeta Pahuja, Geetika Sharma*, P. Lalita Jyotsna and Richa Chauhan Department of Regional Blood Transfusion Center,
More informationPassenger Lymphocyte Syndrome (case presentation) Dr. Namal Bandara Kings College Hospital
Passenger Lymphocyte Syndrome (case presentation) Dr. Namal Bandara Kings College Hospital Case history 24year Female Known Patient with Wilsons Disease DBD donor Liver Transplantation done on 15/08/2016
More informationAUTOIMMUNE HEMOLYTIC ANEMIA (AIHA); A HEMATOLOGIC PERSPECTIVE AT A TERTIARY CARE HOSPITAL.
The Professional Medical Journal DOI: 10.17957/TPMJ/16.3568 ORIGINAL PROF-3568 AUTOIMMUNE HEMOLYTIC ANEMIA (AIHA); A HEMATOLOGIC PERSPECTIVE AT A TERTIARY CARE HOSPITAL. ashayat@hotmail.com 1. MBBS, M.D
More informationSignificance of Antibodies and appropriate selection of red cells for transfusion Chris Elliott Haematology Service manager
Significance of Antibodies and appropriate selection of red cells for transfusion Chris Elliott Haematology Service manager James Cook University Hospital Friarage Hospital Over the next 30 minutes Bit
More informationPILOT STUDY OF ANTIGEN MATCHING FOR AUTOIMMUNE HEMOLYTIC ANEMIA
PILOT STUDY OF ANTIGEN MATCHING FOR AUTOIMMUNE HEMOLYTIC ANEMIA Sharon Rice & Fred Plapp Saint Luke s Hospital Kansas City, MO CENTRALIZED TRANSFUSION SERVICE Antibody identification Antibody titer Antigen
More informationWhen Selfies Go Bad!
When Selfies Go Bad! Decoding Autoantibodies and Autoimmune Hemolytic Anemia D. Joe Chaffin, MD April 17, 2017 Outline Background Warm autoantibodies and WAIHA Cold autoantibodies and CAD Paroxysmal Cold
More informationNew phase 2 Clinical Trial Enrolling Now
New phase 2 Clinical Trial Enrolling Now Warm Autoimmune Hemolytic Anemia (waiha) and Cold Agglutinin Disease (CAD) Designed for patients Age 18 or older With a primary diagnosis of waiha who had a recurrence,
More informationCase Study. Michael Losos, MD, 1 Diane Hamad, MLS(ASCP), 1 Sarita Joshi, MD, 2 Scott Scrape, MD, 1 Jian Chen, MD, PhD 1 * ABSTRACT
Warm Autoimmune Hemolytic Anemia and Direct Antiglobulin Testing With a False- Negative Result in a 53-Year-Old Man: The DAT Will Set You Free Michael Losos, MD, 1 Diane Hamad, MLS(ASCP), 1 Sarita Joshi,
More informationCorrelation Between Warm Reactive Autoantibodies. And The Monocyte Monolayer Assay
Correlation Between Warm Reactive Autoantibodies And The Monocyte Monolayer Assay 1 ABSTRACT BACKGROUND: The monocyte monolayer assay (MMA) measures the adherence and phagocytosis of sensitized red cells
More informationAUTOIMMUNE HAEMATOLOGICAL DISORDERS DIAGNOSTIC INTRICACIES
AUTOIMMUNE HAEMATOLOGICAL DISORDERS DIAGNOSTIC INTRICACIES Dr.Anjali Kelkar CME in Pathology 2016 8th - 10th July, 2016 1 INTRODUCTION Spectrum of Autoimmune Haematological Disorders: Autoimmune haemolytic
More informationCorrelation between the levels of anti- A/B IgM/IgG antibodies and cord blood bilirubin levels in haemolytic disease of the new-born
Original article Correlation between the levels anti- A/B IgM/IgG antibodies and cord blood bilirubin levels in haemolytic disease the new-born C HEMACHANDRIKA 1* TK SHAANTHI GUNASINGH 2 1Pressor & Head,
More informationGeneral Characterisctics
Anemia General Characterisctics Definition: anemia is a decrease in red blood cells. Happens due to underproduction, increased destruction or loss of red cells. Diagnosis of anemia: Hgb < 135 (men) Hgb
More informationCASE STUDIES IN CLINICAL APPLICATIONS OF THERAPEUTIC PLASMA EXCHANGE
CASE STUDIES IN CLINICAL APPLICATIONS OF THERAPEUTIC PLASMA EXCHANGE Eric Rosa, MLS (ASCP) CM Medical Laboratory Scientist Transfusion Service April 18, 2018 Objectives Explain the process of a therapeutic
More informationEarly View Article: Online published version of an accepted article before publication in the final form.
: Online published version of an accepted article before publication in the final form. Journal Name: Journal of Case Reports and Images in Medicine Type of Article: Case Report Title: Paroxysmal cold
More informationApellis Company Presentation November
Apellis Company Presentation November 2018 1 Forward looking statements Statements in this presentation about future expectations, plans and prospects, as well as any other statements regarding matters
More informationSupplementary Table 1. The distribution of IFNL rs and rs and Hardy-Weinberg equilibrium Genotype Observed Expected X 2 P-value* CHC
Supplementary Table 1. The distribution of IFNL rs12979860 and rs8099917 and Hardy-Weinberg equilibrium Genotype Observed Expected X 2 P-value* CHC rs12979860 (n=3129) CC 1127 1145.8 CT 1533 1495.3 TT
More informationALL Blood Transfusion samples must be hand-written in accordance with the Trust's Blood Administration Protocol
Blood Transfusion Routine Investigations ALL Blood Transfusion samples must be hand-written in accordance with the Trust's Blood Administration Protocol Full Group & Screen 2ml EDTA Can be stored at 2-8C
More informationDirect Antiglobulin Test (DAT)
Exercise 8 Direct Antiglobulin Test (DAT) Objectives 1. State the purpose for performing the DAT. 2. State what a positive DAT indicates. 3. List the reagents which are used for performing the DAT. 4.
More informationApellis Company Presentation October
Apellis Company Presentation October 218 1 Forward looking statements Statements in this presentation about future expectations, plans and prospects, as well as any other statements regarding matters that
More informationAnemia. A case-based approach. David B. Sykes, MD, PhD Hematology, MGH Cancer Center June 8, 2017
Anemia A case-based approach David B. Sykes, MD, PhD Hematology, MGH Cancer Center June 8, 2017 Recognizing trends Learning Objectives MCV, RDW, Ferritin, LDH, Reticulocytes Managing complex patients 1.
More informationDAT-B 2015 Direct Antiglobulin Test
EVALUATION DAT-B 2015 Direct Antiglobulin Test INSTITUTION: ATTENTION: CAP NUMBER: 7181660-01 Kit# 1 KIT INFORMATION: Kit ID: 28002258 10/12/2015 11/16/2015 Next Mailing Date: 2/22/2016 COPIED TO: LAP
More informationDrug induced hemolysis: transfusion management Interactive case study
Drug induced hemolysis: transfusion management Interactive case study Kurt Anseeuw, MD Department of Emergency Medicine ZNA Stuivenberg Antwerp, Belgium Case 1 Male patient, 50 y History Nil Chief Complaint
More informationEssentials of Blood Group Antigens and Antibodies
Essentials of Blood Group Antigens and Antibodies Non-Medical Authorisation of blood Components Nov 2017 East Midlands Regional Transfusion Committee Transfusion Terminology Antigens and Antibodies Antibodies
More informationAnemia (3).ms4.25.Oct.15 Hemolytic Anemia. Abdallah Abbadi
Anemia (3).ms4.25.Oct.15 Hemolytic Anemia Abdallah Abbadi Case 3 24 yr old female presented with anemia syndrome and jaundice. She was found to have splenomegaly. Hb 8, wbc 12k, Plt 212k, retics 12%, LDH
More informationAnemia (3).ms Hemolytic Anemia. Abdallah Abbadi Feras Fararjeh
Anemia (3).ms4.26.2.18 Hemolytic Anemia Abdallah Abbadi Feras Fararjeh Case 3 24 yr old female presented with anemia syndrome and jaundice. She was found to have splenomegaly. Hb 8, wbc 12k, Plt 212k,
More informationApellis Company Presentation March 2019
Apellis Company Presentation March 219 Forward looking statements Statements in this presentation about future expectations, plans and prospects, as well as any other statements regarding matters that
More informationInvestigation and Clinical Management of Patients with a positive DAT with and without Haemolysis. Summary of Significant Changes
This Specification replaces SPN220/2.1 Copy Number Summary of Significant Changes Effective 13/04/17 Hyperhaemolysis section removed, as this is now covered in SOP4743. Paragraph on Paroxysmal nocturnal
More informationThe Journal of International Medical Research 2011; 39:
The Journal of International Medical Research 2011; 39: 934 943 Comparative Evaluation of the Microcolumn Gel Card Test and the Conventional Tube Test for Measurement of Titres of Immunoglobulin G Antibodies
More informationOther Blood group systems
Other Blood group systems Blood group systems Dean, L (2005) Blood Group systems Blood group systems can be divided into: 1- Carbohydrate based systems such as Lewis, P and Ii (ABO system also belong to
More informationUse of the XE-2100 in a Patient with Cold Auto-immune Hemolytic Anemia
Use of the XE-21 in a Patient with Cold Auto-immune Hemolytic Anemia Gudrun STAMMINGER and Lothar BEIER Klinikum Chemnitz ggmbh, Institut für Laboratoriumsmedizin, Flemmingstraße 2, Chemnitz, Germany.
More informationBlood transfusion. Dr. J. Potgieter Dept. of Haematology NHLS - TAD
Blood transfusion Dr. J. Potgieter Dept. of Haematology NHLS - TAD General Blood is collected from volunteer donors >90% is separated into individual components and plasma Donors should be: healthy, have
More informationTransfusion Reactions. Directed by M-azad March 2012
Transfusion Reactions Directed by M-azad March 2012 Transfusion Reactions are Adverse reactions associated with the transfusion of blood and its components Transfusion reactions Non-threatening to fatal
More informationJ MEDICINE 2010; 11 : EVANS SYNDROME
J MEDICINE 2010; 11 : 78-82 EVANS SYNDROME AHMEDUL KABIR 1, JAYANTA BANIK 2, RATAN DAS GUPTA 1, ROBED AMIN 1, A. M. WASIQ FAISAL 3, ASM MAFIDUL ISLAM 4, MOSTOFA KAMAL 5, PINAKI PAUL 6, FAIZUL ISLAM CHOWDHURY
More informationSignificance of Antibodies and appropriate selection of red cells for transfusion. Divis ion of Pat hology
Significance of Antibodies and appropriate selection of red cells for transfusion Bit of basic immunology Serological red cell transfusion reactions Testing and sample timing Problems Coping strategies
More informationAUTOIMMUNE HAEMOLYTIC ANAEMIA AT CHRIS HANI BARAGWANANTH
AUTOIMMUNE HAEMOLYTIC ANAEMIA AT CHRIS HANI BARAGWANANTH ACADEMIC HOSPITAL A RETROSPECTIVE STUDY Mmuso Kgosi Mogwera Student number: 699542 Master in Medicine A research report submitted to the faculty
More informationSignificance of Antibodies and appropriate selection of red cells for transfusion
Significance of Antibodies and appropriate selection of red cells for transfusion James Cook University Hospital Friarage Hospital Bit of basic immunology Serological red cell transfusion reactions Testing
More informationAcute haemolysis and appearance of PNH-like clones in patients with vitamin B12 deficiency and iron deficiency after iron dextran administration
Acute haemolysis and appearance of PNH-like clones in patients with vitamin B12 deficiency and iron deficiency after iron dextran administration Chun-Liang Lin 1, Chin-Chan Lin 1,Wen-Jyi Lo 2,Yu-Chien
More informationCharacteristics, treatment and outcome of warm autoimmune hemolytic anemia
Örebro University School of Medicine Degree Project, 15 ECTS June 2017 Characteristics, treatment and outcome of warm autoimmune hemolytic anemia Version 2 Author: Rickard Hedberg Supervisor: Bertil Uggla,
More informationWarm Autoantibodies in a Patient with Hemophagocytic Lymphohistiocytosis: A Case Report
Warm Autoantibodies in a Patient with Hemophagocytic Lymphohistiocytosis: A Case Report Emily Coberly, MD Department of Pathology and Anatomical Sciences University of Missouri Columbia April 30, 2013
More informationHEMOLY L TIC AN A EMIA
HEMOLYTIC ANEMIA In a healthy person, a red blood cell survives 90 to 120 days in the circulation, so about 1% of human red blood cells break down each day. The spleen (part of the reticulo-endothelial
More informationSUPPLEMENTARY MATERIALS
SUPPLEMENTARY MATERIALS Supplementary Table 1. Demographic and clinical characteristics of the primary Sjögren's syndrome patient cohort Number % Females/males 73/5 93.6/6.4 Age, median (range) years 65
More informationAIMS FELLOWSHIP CURRICULUM TS I TRANSFUSION SCIENCE Core Module
Module Code: TS 1 Module Title: Module Convenor: Patient Based Transfusion Science Dr T Cobain Discipline Committee: Ms Dianne Grey Dr D Roxby Ms D Stern Date Module Outline last reviewed: January 2016
More informationChoosing the Correct Statistical Test
Choosing the Correct Statistical Test T racie O. Afifi, PhD Departments of Community Health Sciences & Psychiatry University of Manitoba Department of Community Health Sciences COLLEGE OF MEDICINE, FACULTY
More informationEpidemiology of hepatitis E infection in Hong Kong
RESEARCH FUND FOR THE CONTROL OF INFECTIOUS DISEASES Epidemiology of hepatitis E infection in Hong Kong DPC Chan *, KCK Lee, SS Lee K e y M e s s a g e s 1. The overall anti hepatitis E virus (HEV) seropositivity
More informationAPPENDIX -1 LIST OF TABLES. 01 The blood group systems Blood group collections
APPENDIX -1 LIST OF TABLES Table 01 The blood group systems. 014 005 02 Blood group collections. 016 008 03 Low frequency antigens: the 700 series. 017 009 04 Frequencies of low frequency antigens. 017
More informationAutoimmune Hemolytic Anemia in Systemic Lupus Erythematosus Patient
Autoimmune Hemolytic Anemia in Systemic Lupus Erythematosus Patient A Case Report Savita Handayani, Hardi Edward Sibagariang Hematology and Oncology Medic Division, Departement of Internal Medicine Medical
More informationTRANSFUSION REACTIONS
14 TRANSFUSION REACTIONS 14.1 INTRODUCTION Transfusion of blood and blood products are reported to cause reactions during or after procedure specially in patients who receive multiple transfusions. These
More informationReview Article Red Blood Cell Destruction in Autoimmune Hemolytic Anemia: Role of Complement and Potential New Targets for Therapy
BioMed Research International Volume 2015, Article ID 363278, 11 pages http://dx.doi.org/10.1155/2015/363278 Review Article Red Blood Cell Destruction in Autoimmune Hemolytic Anemia: Role of Complement
More informationAutoantibodies with Mimicking Specificity Detected by the Dilution Technique in Patients with Warm Autoantibodies
Original Article Transfusion Medicine Ann Lab Med 2013;33:343-348 ISSN 2234-3806 eissn 2234-3814 Autoantibodies with Mimicking Specificity Detected by the Dilution Technique in Patients with Warm Autoantibodies
More informationwith AIH, or in whom this diagnosis is suspected, are referred to this centre and have provided a unique opportunity to study a great many cases.
BRITISH MEDICAL JOURNAL VOLUME 282 2 JUNE 1981 223 Occasional Revziezv MEDICAL PRACTICE Autoimmune haemolysis: an 18-year study of 865 cases referred to a regional transfusion centre R J SOKOL, S HEWITT,
More informationIMMUNOHEMATOLOGY. Bio-Rad Laboratories. 2014/2015 Catalog. The Complete Solution for Safe Transfusion. ID-System
Bio-Rad Laboratories IMMUNOHEMATOLOGY ID-System 2014/2015 Catalog The Complete Solution for Safe Transfusion ID-System 2014/2015 Catalog The Complete Solution for Safe Transfusion Bibliography [1] Issitt,
More informationClinical features and outcomes of autoimmune hemolytic anemia: a retrospective analysis of 32 cases
VOLUME 46 ㆍ NUMBER 2 ㆍ June 2011 THE KOREAN JOURNAL OF HEMATOLOGY ORIGINAL ARTICLE Clinical features and outcomes of autoimmune hemolytic anemia: a retrospective analysis of 32 cases Seung-Woo Baek, Myung-Won
More informationHEMOLYTIC ANEMIA Anemia of increased destruction Normochromic, normochromic anemia Shortened RBC survival Reticulocytosis - Response to increased RBC
HEMOLYTIC ANEMIAS Edited by: GR. Bahoush, MD. HEMOLYTIC ANEMIA Anemia of increased destruction Normochromic, normochromic anemia Shortened RBC survival Reticulocytosis - Response to increased RBC destruction
More informationWinter Surge. Dr. KM Chow. Prince of Wales Hospital
Winter Surge Dr. KM Chow Prince of Wales Hospital A Call from Chemical Pathology Plasma potassium 7.9 mmol/l From a 53-year-old outpatient after blood test at the Blood Taking Centre Headache for Bed Availability
More informationBased in Lyon, France, Alvedia provides a full range of canine, feline, swine & equine diagnostics products since 2004.
Alvedia is a company specialized in the field of veterinary diagnostics, providing veterinary health professionals with the most advanced and innovative immunochromatographic technology. Based in Lyon,
More informationImmunohematology Case Studies
Immunohematology Case Studies 2016-2 Nicole Thornton International Blood Group Reference Laboratory (IBGRL) NHS Blood and Transplant Bristol, United Kingdom nicole.thornton@nhsbt.nhs.uk Clinical History
More informationUnderstanding the Complement Cascade and Its Role in Cold Agglutinin Disease. 1 M-CAgD-US-3006 February 2018
Understanding the Complement Cascade and Its Role in Cold Agglutinin Disease 1 February 2018 Instructions This information is provided as an educational resource for healthcare providers. It is not intended
More informationUniversity of Pretoria
University of Pretoria Serodiagnostic Procedures Performed in the Department of Immunology Dr Pieter WA Meyer 1.Autoimmune Diseases Automated Anti-nuclear antibodies Anti-gliadin/ tissue transglutaminase
More informationIntroduction. Case Reports 18/08/2011. Intravenous Immunoglobulin (IVIg) Prepared from large pools of plasma.
Introduction Intravenous Immunoglobulin (IVIg) Prepared from large pools of plasma. By Friend Maviza Consists of IgG with minimal levels of other immunoglobulin classes. IVIg is prescribed for several
More informationAutoimmunity in CLL. Anne Silva, MD Hematology Fellows Conference
Autoimmunity in CLL Anne Silva, MD Hematology Fellows Conference Case Presentation Mr. M is a 62 year old male with multiple medical problems including pulmonary sarcoidosis on steroids, was incidentally
More informationStatistical questions for statistical methods
Statistical questions for statistical methods Unpaired (two-sample) t-test DECIDE: Does the numerical outcome have a relationship with the categorical explanatory variable? Is the mean of the outcome the
More informationAStudyonColdAgglutininsinMalariafromaTertiaryCareHospitalofSouthIndia
Global Journal of Medical research: c Microbiology and Pathology Volume 14 Issue 1 Version 1.0 Year 2014 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc.
More informationHAEMATOLOGY. Test Name Status Result Unit Reference Interval HbA1c (Glycated Haemoglobin) 5.4 % Method : HPLC Sample Type : Whole Blood EDTA
Institution Report Date 25-Dec-2016 05:59 PM HAEMATOLOGY HbA1c (Glycated Haemoglobin) 5.4 % 4.5-6.0 Method : HPLC Sample Type : Whole Blood EDTA REMARKS In vitro quantitative determination of HbA1c in
More informationWinter Surge. Dr. Winston Fung, KM Chow. Prince of Wales Hospital
Winter Surge Dr. Winston Fung, KM Chow Prince of Wales Hospital A Call from Chemical Pathology Plasma potassium 7.9 mmol/l From a 53-year-old outpatient after blood test at the Blood Taking Centre Headache
More informationImmunohematology (Introduction)
Modified from Serotonin version Immunohematology (Introduction) References: -Blood Groups and Red Cell Antigens (Laura Dean) -Cellular and molecular immunology, 8 th edition Introduction to replace blood
More informationAntibody elutions in Thai patients with a positive direct antiglobulin test
ORIGINAL ARTICLE Antibody elutions in Thai patients with a positive direct antiglobulin test Oytip Nathalang 1, Pramote Sriwanitchrak 1, Jintana Tubrod 2, Pawinee Kupatawintu 2 1 Department of Medical
More information8/28/2018. Disclosures. Objectives. None. Automation to PreciseType and Everything in Between
Automation to PreciseType and Everything in Between Jessie Singer MT(ASCP) Transfusion Medicine Children s Hospital Los Angeles None Disclosures Objectives Describe the application of molecular testing
More informationAutomation to PreciseType and Everything in Between. Jessie Singer MT(ASCP) Transfusion Medicine Children s Hospital Los Angeles
Automation to PreciseType and Everything in Between Jessie Singer MT(ASCP) Transfusion Medicine Children s Hospital Los Angeles None Disclosures Objectives Describe the application of molecular testing
More informationLaboratory Results Summary
Laboratory Results Summary Laboratory CO1033AI Test event 2 of 3 CAP LAP Number 7181660 Page Print Date 9 / 10 2008/Jul/04 Program Syphilis (SYPH435) Results Deadline 2008/Jun/24 Subscription ID Analyte
More informationHow the Innate Immune System Profiles Pathogens
How the Innate Immune System Profiles Pathogens Receptors on macrophages, neutrophils, dendritic cells for bacteria and viruses Broad specificity - Two main groups of bacteria: gram positive, gram-negative
More informationImmune Mediated Haemolytic Anaemia Secondary to Sheathed Microfilaria A Case Report
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 5 (2017) pp. 603-607 Journal homepage: http://www.ijcmas.com Case Study https://doi.org/10.20546/ijcmas.2017.605.069
More informationLecture 2. Immunoglobulin
Lecture 2 Immunoglobulin Objectives; Define secretary IgA Describe structure & functions of IgM Compare the antigenic receptor of B lymphocyte Assess the role of IgE in Atopy Distinguish between Isotype,
More informationInfection and Immune Reconstitution: The NEW Forms
Infection and Immune Reconstitution: The NEW Forms Marcie Tomblyn, MD, MS Assistant Professor, UMN Assistant Scientific Director, CIBMTR Minneapolis February 16, 2006 Why the changes? Infection data not
More informationLecture 2. Immunoglobulin
Lecture 2 Immunoglobulin Objectives; To study the secretary IgA To know the structure & functions of IgM The antigenic receptor of B lymphocyte The role of IgE in Atopy The difference between Isotype,
More informationGuidelines for the Management of Urgent Red Cell Transfusion and Situations when Serological Compatibility cannot be Assured
1. Aim This document aims to provide guidance on the selection of the most appropriate red cells when the urgency of the need for transfusion dictates that serologically compatible blood will not be available.
More informationQuality of End-of-Life Care in Patients with Hematologic Malignancies: A Retrospective Cohort Study
Quality of End-of-Life Care in Patients with Hematologic Malignancies: A Retrospective Cohort Study David Hui, Neha Didwaniya, Marieberta Vidal, Seong Hoon Shin, Gary Chisholm, Joyce Roquemore, Eduardo
More informationWhy the Hospital Transfusion laboratory may challenge the use of O D negative blood in an emergency.
Why the Hospital Transfusion laboratory may challenge the use of O D negative blood in an emergency. Carly Lattimore Blood Bank Team Manager Chesterfield Royal Hospital Why the laboratory may challenge
More informationIt s not just allo-antibodies that a red cell transfusion can stimulate
It s not just allo-antibodies that a red cell transfusion can stimulate Associate Professor Ralph Green Laboratory Medicine RMIT University Melbourne, Australia Transfusion practice Minimise risk of transmitting
More informationAll you wanted to know about transfusion support for transplants
All you wanted to know about transfusion support for transplants Dr Dora Foukaneli NHSBT and Addenbrooke s Hospital Cambridge When / why / why not? What ABO group? Do other groups matter? Transplantation
More informationAdaptive Immunity: Specific Defenses of the Host
17 Adaptive Immunity: Specific Defenses of the Host SLOs Differentiate between innate and adaptive immunity, and humoral and cellular immunity. Define antigen, epitope, and hapten. Explain the function
More informationIschemic Stroke in Critically Ill Patients with Malignancy
Ischemic Stroke in Critically Ill Patients with Malignancy Jeong-Am Ryu 1, Oh Young Bang 2, Daesang Lee 1, Jinkyeong Park 1, Jeong Hoon Yang 1, Gee Young Suh 1, Joongbum Cho 1, Chi Ryang Chung 1, Chi-Min
More informationDairion Gatot, Soegiarto Ganie, Savita Handayani. Divisi Hematologi & Onkologi Medik Departemen Ilmu Penyakit Dalam FK-USU/RS H.Adam Malik Medan 2009
HAEMOLYTIC ANAEMIA Dairion Gatot, Soegiarto Ganie, Savita Handayani. Divisi Hematologi & Onkologi Medik Departemen Ilmu Penyakit Dalam FK-USU/RS H.Adam Malik Medan 2009 WHEN BY THEN Hb 9 g% transfusion
More informationManifestation of Antiphospholipid Syndrome among Saudi patients :examining the applicability of sapporo Criteria
Manifestation of Antiphospholipid Syndrome among Saudi patients :examining the applicability of sapporo Criteria Farjah H AlGahtani Associate professor,md,mph Leukemia,Lymphoma in adolescent,thromboembolic
More information2. Liver blood tests and what they mean p2 Acute and chronic liver screen
Hepatology referral pathways for GP 1 Scope For use within hepatology Contents 2. Liver blood tests and what they mean p2 Acute and chronic liver screen p2 Common reasons for hepatology referral 3. Raised
More informationFOR PUBLIC CONSULTATION ONLY RITUXIMAB FOR CYTOPENIA FROM PRIMARY IMMUNE DEFICIENCY
RITUXIMAB FOR CYTOPENIA FROM PRIMARY IMMUNE DEFICIENCY QUESTION(S) TO BE ADDRESSED: What is the evidence for the clinical and cost effectiveness for rituximab for the management of auto-immune cytopenia
More informationThe pathology of autoimmune haemolytic anaemia
J Clin Pathol 1992;45:1047-1052 The pathology of autoimmune haemolytic anaemia R J Sokol, D J Booker, R Stamps Introduction Autoimmune haemolytic anaemia (AIHA) can be defined as a reduced haemoglobin
More informationAppendix to Notification Letter for rituximab and eltrombopag dated 20 February 2014
Appendix to Notification Letter for rituximab and eltrombopag dated 20 February 2014 The notification letter which contains details of the decision to widen the restriction criteria for rituximab and eltrombopag
More informationImmunohematology (Introduction) References: -Blood Groups and Red Cell Antigens (Laura Dean) -Cellular and molecular immunology, 8 th edition
Immunohematology (Introduction) References: -Blood Groups and Red Cell Antigens (Laura Dean) -Cellular and molecular immunology, 8 th edition Introduction to replace blood lost by hemorrhage or to correct
More informationRed Cell Immunohaematology
Red Cell Immunohaematology USER GUIDE 2008 User Guide Author(s): Vanessa Winfield Page 1 of 23 Contents This guide NHS Blood and Transplant Management of Red Cell Immunohaematology Quality statement Complaints
More informationCIBMTR Center Number: CIBMTR Recipient ID: RETIRED. Today s Date: Date of HSCT for which this form is being completed:
Juvenile Idiopathic Arthritis Pre-HSCT Data Sequence Number: Date Received: Registry Use Only Today s Date: Date of HSCT for which this form is being completed: HSCT type: autologous allogeneic, allogeneic,
More informationERROR CORRECTION FORM
Juvenile Idiopathic Arthritis Pre-HSCT Data Sequence Number: Registry Use Only Date of HSCT for which this form is being completed: HSCT type: autologous allogeneic, allogeneic, syngeneic unrelated related
More informationTest Name Results Units Bio. Ref. Interval
135091662 Age 45 Years Gender Male 29/8/2017 120000AM 29/8/2017 100215AM 29/8/2017 110825AM Ref By Final RHEUMATOID AUTOIMMUNE COMREHENSIVE ANEL ANTI NUCLEAR ANTIBODY / FACTOR (ANA/ANF), SERUM ----- 20-60
More informationInvestigation of patients with autoimmune haemolytic anaemia and provision of blood for transfusion
602 62 Clin Pathol 1995;48:602-610 ACP Broadsheet No. 145 July 1995 This Broadsheet has been prepared by the authors at the invitation of the Association of Clincial Pathologists who reserve the copyright.
More information