Results of the implementation of the Rabies co-financed eradication programme in 2011 ESTONIA
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1 Results of the implementation of the Rabies co-financed eradication programme in 2011 ESTONIA Veterinary and Food Board of Estonia SCFCAH, September 2012, Brussels Photo:: Jan Siimson
2 Legal framework EU legal basis: Commission Decision 2010/712/EC Commission Decision 2011/862/EU Relevant Estonian legal acts: Infectious Animal Disease Control Act (16 June 1999) Regulation of Minister of Agriculture No 67 (20. November 2000) Rules for Rabies Prevention State Program of Rabies Eradication approved by Degree of DG of Veterinary and Food Board no. 63 State Program of Monitoring and Surveillance of Infectious Diseases approved annually by CVO
3 Spread of sylivatic rabies in Estonia autumn: start of OVF 500 Cases Years Last human case in
4 Species affected Reservoir animals- raccoon dogs, red foxes Infection across the species :
5 OV in years First large-scale OV, autumn 2005 in km2 area, northern part of Estonia vaccination in total territory of Republic Area suitable for dropping ~ km2 Two seasonal campaigns: spring (May, early June) autumn (September, October) Baiting density: 20 baits/km2 of SAG 2 baits Distribution: manually from fixed-wing planes Dropping lines interval: 600 m No additional hand- distribution by land Efficiency control in target animals Disease surveillance: all-the-year-round
6 Rabies cases in
7 OV of wildlife in 2011(1) OV carried out only in buffer-zone with neighbouring countries Depth of immune belt: 30 km in north east Russian border near river Narva 50 km in south-east Estonian-Russian land border 20 km in south between Estonia-Latvia Total territory of buffer zone sq km. Urban areas, roads, water bodies and wet fields excluded from area.
8 OV of wildlife in 2011(2) 2 campaigns per year: Spring - from 16 th to 26 th May Autumn- 12 th to 25 th of September Vaccine baits used: Rabigen SAG2 Baiting density: 20 baits/km baits distributed in total baits in spring baits in autumn Distribution by fixed-wing planes type Cesna 172 Bait- dropping carried out by trained stuff by hand Distance between dropping lines 600m Altitude form ground m Flight speed km/h
9 OV of wildlife in 2011 (3) GPS system (GARMIN 196) for tracking No additional manual distribution 10 samples from all vaccine batches sent to ANSES Nancy for titration, results should be available before start of OV Refrigerated lorry in airfields Continuous checks on maintenance of cold-chain Efficiency control ( July 2011 March 2012) Passive surveillance all year round 9
10 Evaluation of vaccination campaigns efficacy Investigations in VFL Tartu and Tallinn dep. Rabies prevalence continuous passive surveillance active surveillance Methods in use FAT, PCR, bioassay-cc Bait consumption: tetracycline marks in canines Age determination of all tested animals Immunisation: titration of rabies antibodies by ELISA Genotyping of positive cases Sample collection ratio planned: 4 suspected /indicator animals /100 km 2 of total territory 4 animals/100 km 2 for detection of bait-uptake and seroconvertion in OV area
11 Rabies situation in 2011 No of reservoir animals tested for virus: foxes 1214 rac. dogs Number of suspected animals tested for virus: 210 wildlife- 165 domestic- 45 Positive case identified in ANSES- genotype 1 RABV.
12 Results of marker detection in 2011 Hunting density obtained 4,3 animals per 100 sq km. Species Head results Samples collected Pos Neg racoon dog fox Total ~83% of tested animals found positive (~ 80% of foxes, ~ 85% of racoon dogs)
13 Results of seroconvertion in 2011 ELISA (Bio-Rad Platelia) test used (cut-off point 0,3 IU) The average sampling density obtained 4,6 animals per 100 sq km Species Blood Samples results collected Pos Neg racoon dog fox total ~45% of tested animals found positive (~ 40% of foxes, ~ 47% of racoon dogs)
14 Financial aspects 2011 All main costs in total (VAT incl): Vaccine supply + distribution Samples collection Laboratory investigations Information campaign Eligible costs in total (VAT excl.): Maximum amount of Community co-financing (Commission Decision 2010/712/EC as amended by 2011/862/EU): EUR
15 OV in year 2012 Continuation of OV in buffer zone (9350 km2) along the borders with Russian Federation, Latvian Republic Spring campaign enforced 14 th to 22 th of May Autumn campaign begins Distribution strategy, baiting density, vaccine in use similar as in past Monitoring OV (bait consumption, herd immunity) started in July No rabies cases found in 2012 until nowadays.
16 Photo: Sven Zacek Thank you for your attention!
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