(H9N2) Fowl plague. ( Eastreday et al., 1997 ) )Jawetz et al.., 2001 ) domestic birds. (Alexander,1993) (Alexander, 1993; Easterday et al.

Size: px

Start display at page:

Download "(H9N2) Fowl plague. ( Eastreday et al., 1997 ) )Jawetz et al.., 2001 ) domestic birds. (Alexander,1993) (Alexander, 1993; Easterday et al."

Jonas Ramsey
5 years ago
Views:

1 . (). 0Log Fowl plague ( Eastreday et al., 997 ) )Jawetz et al.., 00 ) (lexander,993) (799) (Zhou,et al.,999 ) H5N caged domestic birds Wild birds birds (lexander, 993; Easterday et al.,997) () ( Webster et al.., 99 ) () (lexander,000 ) ). () (Sluis,000 domestic birds Wild birds Subclinical Clinical infection.) Swayne et al., 998) 6

2 ( ( )( (Grist et. al. 979 ; Hawkes, 979 ) 5 Log 3Log Log 0 Log 9Log 5Log 7

3 Log.5 5 ) H 9 N ( H 9 N 7 ) H 9 N ( 0 0 Log 30 (H 9 N ) H 9 N H 9 N 08 8

4 Log / B 7 B H 9 N 7 H 9 N H 9 N 9

5 H 9 N asile H 9 N not Tested T-test 397 (P<0.05) H 9 N (H 5 N ) 799 (6) ( MMWR,997 ) 0 ) ( Oxford, 0

6 )H 9 ( ( MMWR,998 ) H 9 N (Zigger, 999 ; Sluis, ( H 9 N ) 000) ( Peiris et al.,999 ) ) ( )pereira et al., 96( ) ( ) ( ( Smith et al.,980 ) (73) (Jawetz et al., 00) ) H 9 N ( (Beard,99;Swayne et al.,998) ( ) ) H 9 N ( /chicken/iraq //00 (H 9 N ) ) ( (Mukhlis, 978 ; L- Dabbagh,989) 000 H 9 N 0 8 (Halvorson et al., 979 ; Lany et al.,98;shan,996) (Potter, 990 ; Swayne )H 9 ( et al,.998)

7 H 9 N H 9 N 55.5 (White and Fenner, 986 ; Swayne et al.,998) H 9 N. H 9 N H 9 N H 9 N H 9 N 0 H 9 N ) Zigger, 999 ; Sluis 000 ( H 9 N (Turner et al., 98 ; snyder et al., 98) Easterday, B.C. ; Hinshaw, V.S. and Halvorson, D.. (997). Influenza. 0 th (ed.) In: Disease of poultry. Calnek, B.W. ; Barnes, H.J. ; Beard, C.W. ; McDougald, L.R. and Salf, Y.N. (eds.), Iowa State University Press, mes, Iowa, US. Pp.: Grist, N.R. ; Ross, C.. and Bell, E.J. (979). Diagnostic method in clinical virology. 7 th ed. Kamp Hall Bindery. Oxford. Guo, Y.J. ; Krauss, S. ; Senne, D.. ; Mo, I.P. ; Lo, K.S.; Xiong, X.P. ; Norwood, M. ; Shortridge, K.F. ; Webster, R.G. ; Guan, Y. (000). Characterization of the pathogenicity of members of newly established H 9 N influenza virus lineages is sia. Virol., 67: l-dabbagh,.s. (989). study of influenza virus isolated from an outbreak in Baghdad during Thesis, College of Medicine, University of l- Mustansiriya, Iraq. lexander, D.J. (993). Orthomyxovirus infection. In: Virus infections of birds. McFerran, J.B. and Mcnulty, M.S. (eds.). Elsevier Science Publishing Company. New York, US.Pp.: lexander,d.j. (000).The history of avian influenza in Poultry. World Poultry. November (Special Issue: vian Influenza ). Pp.:7-8.

8 Sluis, W.V. (000). I in Southern sia and the middle east. World Poultry.Nov. Special Issue: vian Influenza.Pp.: 5. Snyder, D.B. ; Marquardt, W.W.; Yancey, F.S. and Savage, P.K. (98). n enzyme linked immunosorbent assay for the detection of antibody against avian influenza virus. vian. Dis., 9():36-5. Smith, V.W.; Coackley, W. and Maker, D. (980). The serological response of chickens to an avian influenza virus administered by various routes. Res. Vet.Sci., 9:8-50. Swayne, D.E. ; Senne, D.. and Beard, C.W. (998). vian Influenza. In : Isolation and identification of avian pathogen.swayne, D.E. ; Glisson, J.R. ; Jackwood, M.W. ; Pearson, J.E. and Reed, Turner, R. ; Lathey, J.L. ; Voris, L.P.V. and Belshe, R.B. (98). Serological diagnosis of B virus infection : Comparison of an Enzyme linked Immunosorbent assay and haemagglutination inhibition.j. Clin. Microbiol.,5 (5) : Webster, R.G. ; Bean,W.J. ; Gordman, O.T. ; Chambers, T.M. and Kawaoka, Y.(99). Evolution and ecology of influenza viruses. Microbiol. Rev., 56:5-79. Zhou, N.N. ; Shordidge, F.K. ; Krauss, L.S. and Webster, G.R. (999). Rabid evolution of H 5 N influenza viruses in chicken in Hong Kong. J. Virol., 73 (): Ziggers,D. (999) vian Influenza in Iran unpredictable outbreak with serious losses. World Poultry. 5():9-50. Hawkes, R.. (979). General principles underlying laboratory diagnosis of viral infections. In: Diagnostic procedures for viral, rikettsial and chlamydial infections, 5 th ed. Lennette, E.H. and Schmidt, N.J. (eds.). merican Public Health ssociation, Washington DC. Pp.:-8. Jawetz, E.M.D. ; Melnick, J.L. and delberg s, E.. (00). Orthomyxo viruses (Influenza viruses). In: Medical Micro-bilogy. 0 th ed, Lange Medical Book, McGraw- Hill,Pp.: MMWR.Update (997). Isolation of avian influenza ( H 5 N ) viruses form human Hong Kong may December 997.MMWR-MORB-Mortal - Wkly Rep., 6 (50):0-07. MMWR.Update. (998). Isolation of avian influenza (H 5 N ) viruses from human-hong Kong, MMWR. Morb. Mortal. Wkly. Rep. Jan 9, 6(5-53):5-7. Mukhlis, F.. (978). Studies on recombinants of influenza virus type () : with reference to vaccine preparation. Thesis College of Medicine, University of Baghdad, Iraq. Oxford, J.S. (000). Influenza pandemic of the 0 th century with special references to 98: Virology, Pathology and Epidemiology. Rev. Med. Virol., 0:9-33. Pereira, G.H. ; Tumova, B.; Law, G.V. (965). vian influenza viruses. Bull. Org. Mond. Sante. Bull. Wld. Hlth. Org., 3: Potter, C.W. (990). Influenza. In: Principles and practice of clinical virology. Zuckerman,.J.; Banatvala, J.E. and Pattisom,J.R. (eds.). Printedby John Wiley and Sons Ltd., London.Pp.:

9 Study on infection with avian influenza virus in chicken H..l-Nasrawei.S.l-Bana R.M.l-Khayatt Coll. of Vet. Med, Univ. of L- Qadisiya Coll. of Vet. Med./ Unive of Baghdad bstract Study include isolation of a single viral isolate of avian influenza virus from disease sample collected from egg layering flock suffering from respiratory and digestive infection and decline in egg production. Embryonated chicken eggs 0 days old used for virusallentoic sac route only used for both isolation and propagation of avian influenza virus.the virus was detected in the harvested allantoic fluid after 7 hours of inoculation by haemagglutination of chicken red blood cells as a primary diagnostic method, but viral type and subtype was determined serologically in haemagglutination inhibition (HI) and Complement fixation (CF) tests.vian influenza virus was diagnosed as type () and subtype (H 9 N ) by using reference antiserum to type () virus and reference antiserum and antigen to avian influenza virus subtype (H 9 N ) in (HI) and (CF) tests.avian influenza viral isolates were propagated in embryonated chicken eggs as highest titer by haemagglutination for virus was 0 Log after (6) serial passages. vian influenza virus H 9 N antibodies were detected in chicken sera from whom the virus were isolated. also anti H 9 N antibodies were diagnosed in serum samples collected from chicken flocks for both egg and meat production in different areas by using HI and ELIS tests and the percentage of positive sera were 56.6% from 90 serum samples by using HI test, but by ELIS test were 7. %.

Isolation of H9N2 Subtype of Avian Influenza Viruses during an Outbreak in Chickens in Iran

Isolation of H9N2 Subtype of Avian Influenza Viruses during an Outbreak in Chickens in Iran Mehdi Vasfi Marandi * and Mohammad Hassan Bozorgmehri Fard Poultry Diseases Section, Faculty of Veterinary Medicine,