QUANTIFICATION RAPID VIRUS WHAT S YOUR APPLICATION?
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1 VIRUS COUNTER 3100
2 RAPID VIRUS QUANTIFICATION A novel approach for determining Total Virus Particle concentration that provides precise results in minutes, not days. MORE MEANINGFUL RESULTS Get a quantitative picture of viral growth that s simple, compliant and cost-effective with: Focus: The first and only automated technology specifically developed to rapidly quantify intact virus particles Speed: Results in minutes, not days or weeks required for other methods Flexibility: Use in standard mode or add a 96-well plate autosampler for overnight processing Reliability: Rugged design for industrial settings, including 21 CFR Part 11-capable software for GMP environments Versatility: Multiple detection systems available WHAT S YOUR APPLICATION? Vaccine Production Enables real-time quantification of viral particles in egg- and cell-based systems. Protein Expression Process optimization guided by same-day virus counting. Viral Therapeutics Virus enumeration across research, development and manufacturing cycles. Antiviral Development Tracking the potency of promising new therapies.
3 FASTER TIME TO RESULTS The Virus Counter 3100 dramatically reduces the labor and time involved in quantifying viruses. The Virus Counter is optimized for rapid virus quantification with an analysis time of less than 5 minutes by utilizing a dual fluorescence staining approach. VIRUS COUNTER TECHNOLOGY: HOW IT WORKS 1With the Combo Dye Kit, viral genomes (and nucleic acids in general) and proteins in viral capsids are stained with a combination of equilibrium dyes that fluoresce in the yellow and red regions of the visible spectrum. This combination approach to staining allows for the detection of a wide variety of viruses using a simple no-wash assay. 2 In the Virus Counter, by using proprietary fluidics technology, stained viral particles are hydrodynamically focused into a narrow nanostream and passed through a laser intercept point. 3 4 Emitted fluorescence is detected on two separate optical channels where optical compensation hardware and software elements correct for any crosstalk between channels. Fluorescence bursts on each channel are counted as a function of time. When fluorescence bursts are simultaneously observed on both the nucleic acid and protein emission channels, the simultaneous event is counted as an intact virus particle. The number of simultaneous events counted during the analysis time is used in combination with sample flow rate to calculate the concentration of virus particles per milliliter of sample. Since ViroCyt s proprietary nanofluidics design ensures that sample flow rate is accurately and precisely measured in real-time, there is no need for an internal calibrant bead such as those used in typical counting assays. FLU A DILUTION Log (vp/ml) Autosampler 3100 y = -1.0x R 2 = Log Dilution Factor Standalone 3100 y = -0.95x R 2 = Serial dilutions of Flu A virus quantified using Virus Counter 3100 in both standalone and autosampler modes show excellent correlation.
4 VERSATILE TECHNOLOGY ENHANCED WORKFLOW The addition of the optional 96-well plate autosampler allows overnight sample processing, dramatically reducing hands-on time. An intuitive software interface provides unsurpassed instrument control and layout management, including the ability to create master templates. Additional features: User-selectable plate cooling maintains sample stability Walk-away automation frees staff for other tasks Reagent and waste container volumes ensure up to 15 hours of continuous operation Easy-to-use graphical user interface provides a flexible and information-rich working environment Compact vertical design minimizes bench space Secure cabinet enclosure isolates sample plate from environmental contaminants SPECIFICATIONS PRODUCT DETAILS DIMENSIONS WEIGHT Virus Counter 3100 Computer Autosampler Shelf Compact, purpose-built system quantifies total intact virus particles in minutes Virus Counter-compatible software in standalone, autosampler and 21 CFR Part 11 versions Windows OS 96-well deep well format Temperature control Space-saving, heavy duty aluminum shelf for placing Virus Counter above the autosampler Height 11 in / 27.9 cm Width 17 in / 43.2 cm Depth 16.5 in / 51.9 cm Standard laptop Height 18 in / 45.7cm Width 13 in / 33 cm Depth 21 in / 53.3 cm Height 21 in / 53.3 cm Width 18 in / 45.7 cm Depth 23 in / 58.4 cm 29 lbs (13.2 kg) 5.2 lbs (2.3 kgs) 47.3 lbs (21.5 kgs) 60 lbs (27.2 kgs) GET THE RESULTS YOU NEED Call , or visit virocyt.com.
5 VERSATILITY: MORE THAN YOU CAN COUNT Pox IBR BVD HCV HSV Real-time quantification of viruses and virus-related products in any setting: Research and development Process scale-up Production Harvest Purification RSV VSV MVA CMV VLPs Polio VEEV Ebola Sendai Hazara Dengue Rubella Vaccinia Measles Pichinde Influenza Rotavirus Lentivirus Arenavirus Enterovirus Baculovirus Coronavirus Yellow Fever Coxsackievirus Parainfluenza Paramyxoviridae 2100 Central Avenue, Suite 103 Boulder, CO
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