Influenza B virus, Monoclonal antibody Influenza vaccine
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1 Key words: Influenza B virus, Monoclonal antibody Influenza vaccine
2 Fig. 1 Number of hospitalized patients due to Influenza B virus infection * Total number of hospitalized patients due to infectious diseases Table 1 Inpatients' HI titers with Influenza B virus infection *; Positive culture a; Acute-phase * *; Nosocomial infection c; Convalescent-phase
3 Table 2 Hospitalized cases with Influenza B virus infection * Positive culture * * Vaccine containing B/Singapore/222/79 u unknown, H3N, and H1N1 Fig. 2 Age distribution of patients with Influenza B virus infection Table 3 Antigenic analysis of Influenza B viruses isolated in 1982
4 Table 4 Antigenic analysis of influenza B virus by monoclonal antibodies specific for determinants on the hemagglutinin of B/Oregon/5/80 Hemagglutination inhibition patterns a; less than 32 b; inhibited hemagglutination by monoclonal antibodies at a low dilution c; HI titer against B/Oregon/5/80
5 ) Stuart-Harris, C.H. & Schild, G.C.: Tnfluerza, The Viruses and the Disease, 1st Ed., Edward Arnold, London, p ) Brocklebank, J.J., Court, S.D.M., etal.: Influerza A infection in children. Lancet, 2: , ) Lennette, E.H. & Schnidt,N.J.: Diagnostic Procedures for Viral and Rickettsial Diseases, 4th Ed., Amer. Public. Health Assoc. Incorp., New Yrok, 1969, p ) Schulman, M., Wilde, C.D. & Kohler, G.: A better cell line for making hybridomas secreting specific antibodies. Nature, 276: , ) Kohler, G. & Milstein, C.: Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol., 6: , ) Webster, R.G., Kendal, A.P. & Gerhard, W.: Analysis of antigenic drift in recently isolated influenza a (HINI) viruses using monoclonal antibody preparations. Virology, 96: , 13) Price, D.A., Postlethwaite, R.J., et al.: Influenza AZ infections presenting with febrile convulsions and gastrointestinal synptoms in young children, Clin. Pediatr., 15: , ) La Montagne, J.R.: Fron the national institute of allergy and infectious diseases, summary of a workshop on influenza B viruses and Reye's syndrome. J. Infect. Dis., 142: , ) Lu Bao-Lan, Webster, R.G., Brown, L.E. & Nerone, K. Heterogeneity of influenza B viruses, Bull, W.H.O., 1983 (in press).
6 Influenza B Outbreak in Pediatrics and HA Analysis by Monoclonal Antibody Norio SUGAYA & Keiko MITAMURA Department of Pediatrics, Nippon Kokan Hospital Yoshinao TAKEUCHI & Hatsuno SANO Department of Pediatrics, School of Medicine, Keio University Kuniaki NEROME & Masatoshi ISHIDA National Institute of Health Influenza B outbreak occured in Japan from Jan. to Feb. of 1982 and 1 million and 680 thousands were affected with Influenza. In order to elucidate the impact of Influenza B outbreak on pediatric patients virus isolation by embryonated eggs and HI test for hospitalized patients were performed. Two Influenza B viruses from hospitalized patients and eight from outpatients were isolated. Among 42 hospitalized patients with infectious diseases, nine became sero-positive against Influenza B virus by HI test. Our result showed that during the outbreak at least 20% of patients due to infectious diseases were associated with Influenza B virus. They were all younger than six and had no history of Influenza vaccination. Their chief complaints were FUO, bronchitis, bronchiolitis, laryngitis and bloody sputa. Our virus isolates were analyzed by HI test with ferret convalescent sera and they showed similar HI pattern with B/Kanagawa/3/76. But with monoclonal antibody they were considerably different from B/Kanagawa/3/76 and also B/Singapore/222/79 which was the vaccine strain of Here we showed the importance of monoclonal antibody on selecting appropriate vaccine strain.
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56 pp.109-1162006 6. 1 2 1 2 10 3 pfu A B 2004/2005 1500 1700 120 1000 1500 420 50 Viral Shedding Frank 1975 1980 Houston Family Study 12 4 A 110-8645 2 23 16 TEL 03-3833-8381 FAX 03-3831-9488 E-mail mitamura@eijuhp.com
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