Proposal for the Coronavirus measures

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1 Proposal for the Coronavirus measures Contents Introduction Point - Approach of prevent level by equipment About company Test Reports Certifications DND Electronics. Co. Ltd.

Introduction Since the Coronavirus has been discovered from the chickens for the first time in 1930, it has been discovered consistently from the dogs, pigs, birds, and human in 1960.

The Coronavirus enters a host, reduplicates its RNA, and makes necessary protein. In 2012, after the Coronavirus has been discovered in Saudi Arabia, It has intensively broken out in middle east area.

The incubation period is about one week, and it is transmitted through the medium of air by coughs and sneezes.

but, It features accompanying the acute kidney injury unlike SARS and according to the research, it has six times higher fatality rate than SARS.

2 Introduction Since the Coronavirus has been discovered from the chickens for the first time in 1930, it has been discovered consistently from the dogs, pigs, birds, and human in It has a ssrna as the genetic material, and encrusted with skin. Its genome size is 26~32kb, and that is the large sized genome among the RNA virus. The Coronavirus enters a host, reduplicates its RNA, and makes necessary protein. In 2012, after the Coronavirus has been discovered in Saudi Arabia, It has intensively broken out in middle east area. This is similar to SARS(Severe Acute Respiratory Syndrome), caused nearly 800 deaths, was spread all over the world from Asia in The incubation period is about one week, and it is transmitted through the medium of air by coughs and sneezes. Also the virus cause severe respiratory symptoms in common with SARS, like a high fever, cough, and difficulty breathing, etc. but, It features accompanying the acute kidney injury unlike SARS and according to the research, it has six times higher fatality rate than SARS. The Coronavirus shows a fatal aspect far more than SARS. So, the study about vaccine for the Coronavirus is ongoing, and it is expected to be developed by the end of the year. Therefore, taking the medicine called Echinaforce is the best way for the present, and the Echinaforce Protect Tab, which is the same medicine, is produced by HanWha Pharma Co., Ltd. in Korea. Also to prevent the virus, Disinfectant Generator, which can remove the various pathogenic bacteria, is used. It is the way to generate disinfectant water through electrolyzing a little amount of diluted salt and HOCl. The solution generated from the device has a strong and widespread sterilizing power, and these systems are being used to prevent from various infectious disease by sterilizing hands before and after eating at school, university, and government branch over 6,000.

Approach of prevent level by equipment [Equipment] Disinfectant Generator Through sterilizing hands which are the main route of various disease, the viral germs are removed by 99.9%.

3 Approach of prevent level by equipment [Equipment] Disinfectant Generator Through sterilizing hands which are the main route of various disease, the viral germs are removed by 99.9%. Especially, it is the most important that before and after eating, after using a toilet, and management of go in and out. It also be used effectively at the places where there are lots of children or many people are moving. Only sterilizing hands is possible to prevent about 90% of various infectious disease and 70% of food poisoning. Sort NaOCl HOCl Main ingredient Active chlorine concentration Sodium hypochlorite (100/200ppm) ph 7.0~7.5 Neutral electrolyzed water Hypochlorous acid (20~30ppm) ph 5.0~6.5 Weak acidic electrolyzed water Additive Salt ( 88%) Dilute hydrochloric acid (2~6%) Features of NaOCl / HOCl -A colorless and transparent liquid with a weak odor of chlorine like tap water. -Doesn t make any harm even if it enters your eyes or mouth, because it is a safe ingredient. -Minimizing the environmental pollution, because it resolves itself into external environment. -Maintenance cost is saved, because it doesn t need any chemical and soap, etc. -With a widespread disinfectant power, it is already recognized as a safe disinfecting water in the world. Principle: The NaOClean system ensures a reliable supply of electrolyzed disinfectant based highly active Sodium hypochlorite or Hypochlorite. Without affecting the ph level, just electric current passes through tap water mixed with a small amount of Salt (NaCl) or Hydrochloric acid (HCl) in an undivided electrolytic bath and the machine generates activated elements such as NaOCl, HOCl, OH(Hydroxyl radical), O(oxygen), etc.

TEST RESULT (Petri dish) Source: Korea Testing & Research Institute Test solution: NaOCl Escherichia coli E.coli 0-157 bacteria 99.99% 99.99% Initial 7.

4 TEST RESULT (Petri dish) Source: Korea Testing & Research Institute Test solution: NaOCl Escherichia coli E.coli bacteria 99.99% 99.99% Initial 7.5X10 5 CFU/ml Initial 4.1X10 5 CFU/ml Salmonella Typhi Staphylococcus Aureus 99.99% 99.99% Initial 2.7X10 5 CFU/ml Initial 3.6X10 5 CFU/ml Vibrio Parahaemolyticus 99.99% Initial 2.8X10 5 CFU/ml

It also solves the problem with using of existing chemicals. (It is possible to reduce budget as much as $4,700 compared with when using general chemical spray sterilizer.

5 TEST RESULT Source: Korea Testing & Research Institute / Korea Centers for Disease Control and Prevention 10~20 sec. Through hand washing before meals, it can prevent various microbism and manage safety in the food service facilities at school. It also solves the problem with using of existing chemicals. (It is possible to reduce budget as much as $4,700 compared with when using general chemical spray sterilizer.) Before After Bactericidal Spectrum of HOCl From General Bacteria to Virus, having the widespread sterilizing power. Until now, it has been appeared being effective to influenza, SARS, Norovirus, and others. But it is considered to be able to have an effect on the new virus.

6 TEST RESULT Source: Korea Ministry for Food, Agriculture, Forestry and Fisheries <Table1> Disinfection efficacy of Hypochlorous acid water on various food microorganism

7 TEST RESULT Source: Korea Ministry for Food, Agriculture, Forestry and Fisheries <Table1> Disinfection efficacy of Hypochlorous acid water on various food microorganism

8 TEST RESULT Source: Korea Ministry for Food, Agriculture, Forestry and Fisheries <Table1> Disinfection efficacy of Hypochlorous acid water on various food microorganism

9 TEST RESULT Source: Korea Ministry for Food, Agriculture, Forestry and Fisheries 1. Viral inactivation effect -Test object: Influenza A virus (H1N1) - Specimen: Hypochlorous acid solution (Active chlorine concentration 20ppm, ph 6.1) -Test sample: Added and mixed 0.1ml of H1N1 suspension at 1ml of Specimen -Test method: measure the viral infection values of the acting fluid after 15sec, and 1 min. in room temperature. -Control plot: Purified water <table2> Measurement result of Virus infection of Test sample Test Virus Object Log TCID 50 / ml * Initial After 15sec. After 1min. Influenza Virus Specimen 6.5 <1.5 <1.5 Control plot TCID 50 : median tissue culture infectious dose As shown in the above table, Hypochlorous acid was confirmed to inactivate virus in 15 seconds to detection limit valve (<1.5).

air-borne microorganism by spraying Hypochlorous acid solution on air has been performed. Test solution: Hypochlorous acid solution (chlorine concentration 27ppm, ph 5.

10 TEST RESULT Source: Korea Health Industry Development Institute 2. Sterilizing effect for suspension bacteria For the highly effective prevention of various pathogenic microorganism and virus by air-borne infection, The experiment which the removal effect to air-borne microorganism by spraying Hypochlorous acid solution on air has been performed. Test solution: Hypochlorous acid solution (chlorine concentration 27ppm, ph 5.5) Test strain: Staphylococcus aureus ATCC 6538 Experiment test space : 1 m3 transparent acrylic box Experiment test result : The air-borne microorganism has been decreased to 99.96% after 30 minutes by Spraying Hypochlorous acid solution on air with humidifier it was the most reduction ratio result, 99.98% after 60 minutes, and after 90 minutes almost the air-borne microorganism was removed to 99.99%. According to the result it is highly effective to air-borne microorganism by spraying the Hypochlorous acid solution on air with such as humidifier. <table3> The experiment test result of air-borne microorganism removal effect by spraying Hypochlorous acid solution on air Humidification time after spraying (min) After spraying Hypochlorous acid solution (log/m 3 ) ± ± ± ± Decrement (%) initial After 60sec. After 30sec. After 90sec.

11 TEST RESULT Source: Korea Health Industry Development Institute Sterilizing effect for suspension bacteria The test result for infectivity reduction effect against Virus by Hypochlorous acid, reduction effect for allergen matter

12 TEST REPORTS Source: Graduate School of Health Sciences and Nursing, Faculty of Medicine, University of Tokyo Title Author Journal name Cleansing effectiveness of Electrolyzed Water Depending on the Nursing Procedure and Degree of Hand Contamination Yasuko Takahashi, Akemi Takeshita, Miyoko Endo, and Minako Sasaki Biocontrol Science Vol.7, 2002, No.3, page: Abstract Summary We examined the antibacterial effectiveness of hand washing with electrolyzed water (EW) depending on the type of nursing procedure and differences in the degree of bacterial contamination on hands after the procedure. With respect to the bacteria on the hands after diapering (relatively heavy contamination), changing positions (relatively light contamination), and endotracheal aspiration (light contamination), hand washing for 10s and 30s with EW lowered the hand bacterial counts after hand washing to the same level as or lower than that before the procedures. Washing with EW was as effective as washing with medicated liquid soap and 7.5% (w/v) povidone-iodine liquid. Based on these results, we considered that and washing for 10s with EW could be applied to any of the procedures that require direct contact with the patient's skin (diapering) or clothing (changing positions), as well as the procedures involving no direct contact with the patient (endotracheal aspiration). Furthermore, effective washing could be expected in a short period of time, with no rising of resident bacteria to the surface of the hand.

13 TEST REPORTS Source: Journal of the Japanese Food Microbiology Title Author Journal name Using electrolyzed water to sanitize hands Akemi Takeshita Journal of the Japanese Food Microbiology vol. 24, issued in 2007, No. 3, Page : Abstract Summary On investigation about the effect of hand washing using an electrolyzed water, by using an electrolyzed water with soap, it is possible to remove pollutants and It has developed that it can maintain a state of having little germs after hand washing. The virus is discharged in quantity during defecating from the inapparent infectors who are infected with the Noro virus but haven t had any symptoms. So, It is better to wash hands enough after using a public toilet. As disinfecting water which is expected effect of hand washing in a short time, it is useful before meals, once go in and out, and after using a toilet. The electrolyzed water has high safety, and also it is expected to have enough sterilizing effect.

14 RESEARCH METERIAL Related Link some countries Country Japan USA Link

15 Product Line Hand Washing System #1. DES-H200 #2. DES-WS850 #3. Others

Product Line #1. DES-H200 #2. DES-WS850 #3.

Governments List: Sejong City Premier's offices &

Government offices, the National Scientific Investigation

Officials Development Institute, National Defense Navy at

16 Product Line #1. DES-H200 #2. DES-WS850 #3. Others Installation : Schools / Kindergartens / Governments List: Sejong City Premier's offices & Government offices, Daegu Government offices, Masan Government offices, the National Scientific Investigation Laboratory, Fair Trade Commission, Local Government Officials Development Institute, National Defense Navy at the Second Fleet, Office of Education in Seoul City, and more than 6,000 School feeding facilities.

17 Product Line Washing Stand for Sterilizing & Deodorizing #1. DES-H200 #2. DES-WS850 #3. Others

18 Product Line #1. DES-H200 #2. DES-WS850 #3. Others Installation : Food waste disposal plant in Residental facilities, etc. List: Waste disposal place in the apartment complex, The funeral hall in the Incheon Mary s Hospital, Daejeon Wellness hospital, etc.

hygienic management of farms and disinfection of

spraying the disinfecting water in the breeding

- By spraying directly onto livestock using a

19 Product Line #1. DES-H200 #2. DES-WS850 #Others Livestock - It can be used for hygienic management of farms and disinfection of livestock to prevent an infectious problems from the diverse livestock. - It can help to sterilize and deodorize by spraying the disinfecting water in the breeding farms, workspaces and more. - By spraying directly onto livestock using a sprayer, it prevents a disease and maintains cleanliness of the livestock. - Prevention of the variety of viral diseases such as avian, pig influenza. Example of install : Anseong livestock, Aerang Ranch, Soshinburl farm, Beomsan Ranch, Sky Ranch and more.

Applicable References Agricultures -Prevent microorganism such as bacteria and germs through sterilizing action.

-Help to increase a trust of consumer by harvesting environment-friendly agricultural products.

and food reserves. -Maintain freshness and disinfect without destruction of nutrients.

20 Applicable References Agricultures -Prevent microorganism such as bacteria and germs through sterilizing action. -Keep your health by minimizing harmful materials such as agricultural pesticide. -Help to increase a trust of consumer by harvesting environment-friendly agricultural products. (producing good fruit through using Electrolyzed water) Feeding facilities / Restaurants -Provide safe and fresh food through sterilization of seafood and food reserves. -Maintain freshness and disinfect without destruction of nutrients. -Prevent secondary infection through hygienic management of food utensils. Dental Clinic / General Hospitals / Animal hospitals -Improve the oral infection and bleeding by gargling with disinfectants. -Prevent secondary infection through sterilization of medical appliances. -Preserve clean environment through spraying in the air and sterilizing of floor after pet defecation.

Products For washing hand DES-WS850 DES-8000 DES-10K DES-WA100E AC 220, 60Hz (400W) AC 220, 60Hz

4kW) AC 220, 60Hz (3kW) W500 X D500 X H850 W650 X D610 X H1300 W480 X D300 X H490 W520 X D585 X

About 65kg Saline tank: 20L Saline tank: 100L Salt tank: 5kg max.

21 Products For washing hand DES-WS850 DES-8000 DES-10K DES-WA100E AC 220, 60Hz (400W) AC 220, 60Hz (1.4kW) AC 220, 60Hz (1.4kW) AC 220, 60Hz (3kW) W500 X D500 X H850 W650 X D610 X H1300 W480 X D300 X H490 W520 X D585 X H970 About 4L/min About 15~20L/min About 15L/min About 8~11L/min About 35kg About 60kg About 25kg About 65kg Saline tank: 20L Saline tank: 100L Salt tank: 5kg max. pressure: 130bar Once: Salt 2kg (Use) Waste disposal place, School health room, etc. (Use) Washing and sterilizing food materials, utensils, etc. (Use) High-pressure washer in Factories, Feeding facilities, etc.

Products For washing hand DES-P450 DES-WA50 DES-WD40 DES-H200 AC 100~240, free (330W) AC 220, 60Hz (2.

2kW) AC 220, 60Hz (300W) W180 X D486 X H463 W615 X D767 X H1005 W440 X D400 X H930 W1360 X D570 X H1280 About

Saline tank: 4L Saline tank: 50L Saline tank: 45L Tap water X 3 Once: Salt 400g max. pressure: 100bar max.

22 Products For washing hand DES-P450 DES-WA50 DES-WD40 DES-H200 AC 100~240, free (330W) AC 220, 60Hz (2.4kW) AC 220, 60Hz (1.2kW) AC 220, 60Hz (300W) W180 X D486 X H463 W615 X D767 X H1005 W440 X D400 X H930 W1360 X D570 X H1280 About 3~4L/min About 5~6/min About 5L/min About 8~11L/min About 17kg About 50kg About 35kg Nozzle: Disinfectant X 3 Saline tank: 4L Saline tank: 50L Saline tank: 45L Tap water X 3 Once: Salt 400g max. pressure: 100bar max. pressure: 80bar (Use) Personal hospitals, Pet shops, Small restaurants, etc. (Use) High-pressure sterilizing washer in Restaurants, Feeding facilities, etc. (Use) Schools, kindergartens, Governments, Airports, etc.

23 About DND Electronics Co., Ltd. 1. Company Profile Having researched for 30 years in electrolyzed water, D&D Electronics made the first disinfecting water generator in 1996, which produces automatically disinfecting water using only a salt, water and an electricity. Our generator has been serving in many places for health care and hygiene including public schools, restaurants, farms, food processing plants about 6,000 places. In 1983, we developed sterilizing humidifier by electrolysis. In 1994, when a problem of E.coli had been come up in Japan, we offered a solution which is a system about Electrolyzed water. In 1995, we developed the sterilizing vegetable washer by electrolysis. In 1999, we developed the disinfecting water producer. In 2008, we made the first high pressure sterilizing washer. As a result, many schools in Korea are using our system and we rank No.1 in terms of market share in the disinfecting generator. In addition, for home use product is becoming standard equipment in most Korea s apartments. Korea Food and Drug Administration approved our generator for safety and effectiveness. By participating in several international exhibitions, NaOClean has been proven the excellence and it has more powerful than ozone sterilizer while having a superior cost-competitive. We are trying to protect the environment from pollution and help prevent a wide range of illness such as food poisoning and infections. We are trying to be reliable and trustworthy to help improve your health and to become beloved company. Meet the company that improves the quality of your life.

24 2. History Date History Beginning of research into Electrolyzed Neutral water Founded as D&D Electronics Selected as one of the leading venture by the Small Business Administration Selected as one of the promising venture by Gyeonggi-do Obtained ISO Selected as 100 great product Electro-analyzed sanitizing solution Designated as Industry family company Approved as a good products NaOClean by Public Procurement Service Recognized as a great performance machine by the Small Business Administration Developed new technologies by the Minister of Industry Ministry recognition Approved as NEP good products by Industry Ministry Recognized as a sterilized disinfectant machine by Korea Food Research Institute Recognized as a food additives Electro-analyzed sanitizing solution, NaOClean by Korea Food Research Institute Recognized as the Patent of Korea in 41th invention day Launched NaOClean plus the first domestic invention Launched NaOClean-S the first domestic invention Received Inno-Biz certification by the Small Business Administration Launched NaOClean for family use Recognized as a developing innovative technology by the Ministry of Education, Science and Technology Selected as one of the promising export venture by the Small Business Administration

25 3. Test reports (Certificated by KFDA)

26 3. Test reports (Certificated by KFDA)

27 3. Test reports

28 3. Test reports

29 3. Test reports

30 3. Test reports (Materials)

31 3. Test reports (Materials)

32 4. Certifications

33 4. Certifications

Evaluation of Low Temperature Depuration for Reducing Vibrio parahaemolyticus in Oysters

Evaluation of Low Temperature Depuration for Reducing Vibrio parahaemolyticus in Oysters Yi-Cheng Su 1, Daniel Cheney 2, and Andy Suhrbier 2 1 Oregon State University Seafood Laboratory, Astoria, OR 97103