Characteristics of Dental Pulp Stem Cells
|
|
- Amy Lee
- 5 years ago
- Views:
Transcription
1 Pharma&Biotech BioResearch Characteristics of Dental Pulp Stem Cells 15 October 2013 / Speaker: Dr. Isabella Drewelus 16 October 2013 / Speaker: Andrew Winner Lonza Walkersville, Inc., Walkersville, MD / October 2013 Lonza
2 Agenda Introduction to Dental Pulp Stem Cells Review of oral stem cells DPSC Isolation and Cell Culture DPSCs vs. Bone Marrow derived MSCs Intro Materials and Methods Results Conclusions 2
3 Oral Cavity Stem Cells Multiple cell types Vary in multipotency and gene expression Image Courtesy of sciencedirect.com 3
4 Stem Cells from Apical Papilla (SCAP) Isolated from the apical tip of the tooth Found just underneath the enamel organ Some Cementum Matrix formation No recorded myogenic or chondrogenic differentiation Image Courtesy of sciencedirect.com 4
5 Stem Cells from Exfoliated Deciduous Teeth (SHED) Also called milk teeth or baby teeth Easily obtained No known dentin-pulp complex formation 5
6 Dental Pulp Stem Cells DPSCs First described in 2000 by Dr. Song Tao Shi* Usually harvested from adult third molars ( Wisdom teeth) million cells per tooth most bang for the buck Easier to obtain Most multipotent of oral cavity cells Very similar to BM derived MSCs Image Courtesy of sciencedirect.com *Shi, S. and Gronthos, S. (2003), Perivascular Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental Pulp. J Bone Miner Res, 18: doi: /jbmr
7 Dental Pulp Stem Cells DPSCs (Continued) Mesenchymal-like multipotent cells that can differentiate into several cell types Myocardiocytes, Neurons, Myocytes, Osteoblasts, Chondrocytes, Adipocytes and other bone/tissue in the oral cavity Phenotype SSEA4+, OCT3/4+, NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD34-, CD45-, CD90+, CD29+, CD73+, STRO1+ and CD146- Morphology in culture Able to form embryoid bodies-like structures (EBs) in vitro Can differentiate into tissues that have similar characteristics to mesoderm, endoderm and ectoderm Form tube-like structures in a Matrigel assay 7
8 DPSC Differentiation: Compared to MSCs MSCs Osteocytes Chondrocytes Myocytes Adipocytes Neurons DPSCs Osteocytes Chondrocytes Myocytes Adipocytes Neurons Also: Astrocytes Stromal cells 8
9 Isolation of DPSCs* 1. Sterilized teeth are gauze wrapped tooth squeezed in a vise until cracked 2. Remove pulp with sterile tweezers digest with a solution of collagenase type I and dispase for minutes 3. Suspend in culture medium, the centrifuge to pellet 4. Re-suspend the cell pellet in regular growth medium and pass the cells through a strainer to obtain single cell suspensions 5. Cells ready for culture *Gronthos, S. (2000), Postnatal human dental pulp stem cells in vitro and in vivo Proc. Natl. Acad. Sci. USA, /pnas
10 Culture and Expansion of DPSCs Used Poietics DPSC Bullet Kit Growth Media Supplements Nearly identical to culturing MSCs Seeding density is 5,000-6,000 cells per cm 2 Fed 3-4 days after plating Near confluence by day 6 or 7 10
11 Agenda Introduction to Dental Pulp Stem Cells Review of oral stem cells DPSC Isolation and Cell Culture DPSCs vs. Bone Marrow derived MSCs Intro Materials and Methods Results Conclusions 11
12 Experimental Design Use flow cytometry to compare the expression of 5 known markers genes both DPSCs and Bone Marrow MSCs CD-166 (ALCOM): leukocyte cell adhesion molecule commonly used as a hematopoietic stem cell marker CD-105 (Endoglin): membrane glycoprotein commonly used to identify MSCs CD-45 is a protein tyrosine phosphatase, type C that is present on the surface of all hematopoietic cells CD-29 is part of the α3β1 complex that helps define neural lineage differentiation CD34 is a cell surface glycoprotein that is commonly used marker for hematopoietic cells 12
13 Periodontal Ligament Stem Cells (PDLSCs) Fibrous connective tissue Neural and vascular components No recorded myogenic or chondrogenic differentiation Weaker expression of Nanog than other oral cavity stem cells Image Courtesy of studiodentaire.com 13
14 Materials and Methods: Cells and Cell Culture Dental Pulp Stem Cells Poietics hdpsc (PT-5025) Poietics hdpsc BulletKit (PT-3005) 5 Day Culture Mesenchymal Stem Cells Poietics hmsc (PT-2501) Poietics hmsc GM BulletKit (PT-3001) 5 Day Culture 14
15 Materials and Methods: Flow Cytometry DPSCs and MCS both Antibody labeled for: CD-105, CD-166, CD-29, CD-34, CD-45, CD-90 Cells acquired on a BD FACSCanto II flow cytometer Analyzed using FACSDiva Image Courtesy of Imperial College of London 15
16 Results: DPSCs (Figures A) Representative data of FACS performed on human DPSC at passage two. These cell tested positive for CD166, CD105 and CD29 but negative for CD45 and CD34. 16
17 Results: MSCs (Figures B) Representative data of FACS performed on human MSC at passage two. These cell tested positive for CD166, CD105 and CD29 but negative for CD45 and CD34. 17
18 Results (Continued) % Positive CD105 CD166 CD29 CD45 CD34 HDPSC MSC Table 1. Summary of Flow Cytometry Results Flow-cytometric analysis revealed human DPSCs were positive for markers CD166, CD105 and CD29, but negative for CD34 and CD45, suggesting that these cells are mesenchymal-like stem cells. 18
19 Agenda Introduction to Dental Pulp Stem Cells Review of oral stem cells DPSC Isolation and Cell Culture DPSCs vs. Bone Marrow derived MSCs Intro Materials and Methods Results Conclusions 19
20 Conclusions Stem Cells harvested from human dental pulp represent a less invasive and more affordable source of stem cells for research and possibly even future therapeutic purposes. Flow-cytometric analysis revealed human DPSCs were positive for markers CD166, CD105 and CD29, but negative for CD34 and CD45, suggesting that these cells are mesenchymal-like stem cells. 20
21 Poietics DPSC and hmsc Cat. No. Description Quantity PT-5025 Human Dental Pulp Stem Cells 1M >= 1 Million Cells / cryoamp PT-2501 Human Mesenchymal Stem Cells 1M >= 1 Million Cells / cryoamp PT-3005 DPSC BulletKit (Pt-3927 & PT-4516) 1 Kit PT-3001 MSCGM BulletKit (PT-3238 & PT-4105) 1 Kit 21
22 References 1) Gronthos, S. (2000), Postnatal human dental pulp stem cells in vitro and in vivo Proc. Natl. Acad. Sci. USA, /pnas ) Shi, S. and Gronthos, S. (2003), Perivascular Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental Pulp. J Bone Miner Res, 18: doi: /jbmr ) Huang, GTJ. (2009) Mesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources J Dent Res. USA 2009 September; 88(9): ) Pierdomenico, Laura et al. (2005) Multipotent Mesenchymal Stem Cells With Immunsupressive Activity can Be Easily Isolated from Dental Pulp. Transplantation 80(6): ) 3 Gluhak-Heinrich, J. et al. (2012) Bmp2 Is Required for Odontoblast Differentiation and Pulp Vasculogenesis JDR 91: ) 4 Whitney GS, Starling GC, Bowen MA, et al. (1995). "The membrane-proximal scavenger receptor cysteine-rich domain of CD6 contains the activated leukocyte cell adhesion molecule binding site.". J. Biol. Chem. 270 (31): ) 5 Kaplan R, Morse B, Huebner K, et al (September 1990). "Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain". Proc. Natl. Acad. Sci. U.S.A. 87 (18): ) 6 Goodfellow PJ, Nevanlinna HA, Gorman P, Sheer D, Lam G, Goodfellow PN (July 1989). "Assignment of the gene encoding the beta-subunit of the human fibronectin receptor (beta- FNR) to chromosome 10p11.2". Ann Hum Genet 53 (Pt 1):
23 How to Order or Ask Questions order to: or For order-related questions, contact Customer Service U.S : (toll free) or order.us@lonza.com Europe: or order.europe@lonza.com For scientific support please contact U.S: or scientific.support@lonza.com Europe: or scientific.support.eu@lonza.com Visit our website for more information 23
24 Thank You! Questions?
Stem Cells : A Review
Stem Cells : A Review Abhijeet Alok 1, Indra Deo Singh 2, Shivani Singh 3, Mallika Kishore 4 1- Senior Lecturer, Department of Oral Medicine and Radiology, Sarjug Dental College and Mata R Devi Dental
More information2.79J/3.96J/BE.441/HST522J DENTAL TISSUE REPLACEMENT AND REGENERATION
Massachusetts Institute of Technology Harvard Medical School Brigham and Women s/massachusetts General Hosp. VA Boston Healthcare System 2.79J/3.96J/BE.441/HST522J DENTAL TISSUE REPLACEMENT AND REGENERATION
More informationIndex. Note: Page numbers of article titles are in boldface type.
Index Note: Page numbers of article titles are in boldface type. A Alginate, tooth-shaped, for constructs, encapsulated pulp cells in, 589 590 Antibiotic paste, triple, change in root length and width
More informationInternational Journal of Scientific Research and Reviews
Review article Available online www.ijsrr.org ISSN: 2279 0543 International Journal of Scientific Research and Reviews Biology Behind The Regeneration: A Short Review Shetty Ganaraj Department of Prosthodontics.
More informationReview Article. Dental stem cells Vashisht N 1, Vashisht D 2
Dental stem cells Vashisht N 1, Vashisht D 2 Review Article 1 Dr Neha Vashisht MDS, Oral Medicine and Radiology Private Practitioner, Shimla 2 Dr Divy Vashisht Senior Lecturer, Prosthodontics H P Government
More informationIn vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells
Original Article In vitro evaluation of isolation possibility of stem cells from intra oral soft tissue and comparison of them with bone marrow stem cells P. Torkzaban 1, A. Saffarpour 2, M. Bidgoli 1,
More informationOne step close to growing a new set of dentures.
One step close to growing a new set of dentures. A team headed up by Dr. Jinhua Yu of the Institute of Stomatology at Nanjing University, China, has continued to lay the foundation for a whole new line
More informationTitle of project: Master s candidate: Y Owusu. Collaborator: Ikbale El Ayachi. Master s Committee: M Donaldson, J Lou, F Garcia-Godoy, G T-J Huang
Title of project: Isolation and Characterization of Periodontal Ligament Stem Cells of Human Deciduous Teeth Master s candidate: Y Owusu Collaborator: Ikbale El Ayachi Master s Committee: M Donaldson,
More informationStem cell-based biological tooth repair and regeneration
Review Special issue CellBio-X Stem cell-based biological tooth repair and regeneration Ana Angelova Volponi 1, Yvonne Pang 1,2 and Paul T. Sharpe 1 1 Department of Craniofacial Development and MRC Centre
More informationSponsored document from Trends in Cell Biology. Stem cell-based biological tooth repair and regeneration
Sponsored document from Trends in Cell Biology Stem cell-based biological tooth repair and regeneration Ana Angelova Volponi 1, Yvonne Pang 1,2, and Paul T. Sharpe 1 Paul T. Sharpe: paul.sharpe@kcl.ac.uk
More informationUsing Dental Stem Cells to Regenerate Tooth Tissue and Whole Tooth Replacement Peretz Rapoport
and Whole Tooth Replacement Peretz Rapoport Peretz Rapoport graduated January 2018 with a BS in Biology and is starting Touro School of Dental Medicine in fall 2018 Abstract Irreversible dental problems
More informationCharacterization of Stem and Progenitor Cells in the Dental Pulp of Erupted and Unerupted Murine Molars
University of Connecticut DigitalCommons@UConn Articles - Research University of Connecticut Health Center Research 6-2010 Characterization of Stem and Progenitor Cells in the Dental Pulp of Erupted and
More informationEmerging role of Stem cells of dental origin in dentistry -A Review
Review Article DOI: 10.18231/2395-499X.2017.0005 Ajay Kumar Kapoor 1,*, Seema Thakur 2, Parul Singhal 3, Cheranjeevi Jayam 4 1 Junior Resident, 2 Professor & HOD, 3 Assistant Professor, 4 Senior Lecturer,
More informationIn vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth
Original Article In vitro Growth and Characterization of Stem Cells from Human Dental Pulp of Deciduous Versus Permanent Teeth MR. Baghaban Eslaminejad 1, S. Vahabi 2, M. Shariati 3, H. Nazarian 1 1 Associate
More informationDENTAL STEM CELLS AND THEIR POTENTIAL ROLE IN REGENRATIVE DENTISTRY
Review Article International Journal of Dental and Health Sciences Volume 02,Issue 04 DENTAL STEM CELLS AND THEIR POTENTIAL ROLE IN REGENRATIVE DENTISTRY Apexa B Patel 1 1.BDS, College of dental sciences
More informationstem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products
stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products Stem Cell Qualified Extracellular Matrix Proteins Stem cell research requires the finest
More informationMultilineage Differentiation of Dental Pulp Stem Cells from Green Fluorescent Protein Transgenic Mice
doi: 10.4248/IJOS10015 Multilineage Differentiation of Dental Pulp Stem Cells from Green Fluorescent Protein Transgenic Mice Brian E. Grottkau, P. Prasad Purudappa, Yun-feng Lin* Department of Orthopaedic
More informationTHE USE OF STEM CELLS IN DENTISTRY AND DEVELOPMENTS FOR THE FUTURE BY HEIDI SWINHOE RESEARCH PAPER BASED ON PATHOLOGY LECTURES AT MEDLINK 2014
THE USE OF STEM CELLS IN DENTISTRY AND DEVELOPMENTS FOR THE FUTURE BY HEIDI SWINHOE RESEARCH PAPER BASED ON PATHOLOGY LECTURES AT MEDLINK 2014 Grade Awarded: Pass with Merit ABSTRACT Stem cells have the
More informationInternational Journal of Health Sciences and Research ISSN:
International Journal of Health Sciences and Research www.ijhsr.org ISSN: 2249-9571 Review Article Stem Cells: Biological Solution for Biological Problems Swati Garg *@, Shraddha Bahirwani ***, Prem Kumar
More informationPattern of bone resorption after extraction
Teeth loss Pattern of bone resorption after extraction 50% in 1st year 2/ 3 in first 3 months Reich KM, Huber CD, Lippnig WR, Um C, Watzek G, Tangl S. (2011, 17). Atrophy of Residual Alveolar Ridge following
More informationstem cell biology has become an important field for the understanding of
critical reviews IN oral biology & MedIcINe G.T.-J. Huang 1 *, S. Gronthos 2, and S. Shi 3 1 University of Maryland, College of Dental Surgery, Dental School, Department of Endodontics, Prosthodontics
More informationSTEM CELLS: THEIR EVOLUTION, PROPERTIES, TYPES AND ROLE IN THE FUTURE OF DENTISTRY A REVIEW
Review Article STEM CELLS: THEIR EVOLUTION, PROPERTIES, TYPES AND ROLE IN THE FUTURE OF DENTISTRY A REVIEW AUTHORS : Pushpalatha Mahesh*, Mahesh M. C** ABSTRACT The effective preservation of stem cells
More informationClonetics Cells in Pancreatic Cancer Research
Pharma&Biotech BioResearch Clonetics Cells in Pancreatic Cancer Research 1 April 2014 / Speaker: Andrew Winner 2 April 2014 / Speaker: Dr. Nazim El-Andaloussi Lonza Cologne GmbH, Cologne / 28 March 2013
More informationUnique Journal of Medical and Dental Sciences Available online: Review Article STEM CELLS IN DENTISTRY - A REVIEW
ISSN 2347-5579 Unique Journal of Medical and Dental Sciences Available online: www.ujconline.net Review Article STEM CELLS IN DENTISTRY - A REVIEW Arora Alka 1*, Chaudhary Devendra 2, Nagpal Ravi 3, Goel
More informationMesenchymal Stem Cells Derived from Dental Tissues vs. Those from Other Sources: Their Biology and Role in Regenerative Medicine
CRITICAL REVIEWS IN ORAL Biology & Medicine G.T.-J. Huang 1 *, S. Gronthos 2, and S. Shi 3 1 University of Maryland, College of Dental Surgery, Dental School, Department of Endodontics, Prosthodontics
More informationStem Cell & Regenerative Medicine
Research Article Research Article Stem Cell & Regenerative Medicine Proliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients Mohamed
More informationStem Cells: Potential Implications for tooth regeneration and tissue engineering in Dental Science
Reveiw Article Stem Cells: Potential Implications for tooth regeneration and tissue engineering in Dental Science S.S. Sandhu, Meera Nair Centre for Scientific Research & Development, People s Campus Bhanpur,
More informationThe Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth
Mojarad F., et al. J Dent Shiraz Univ Med Sci., 2016 December; 17(4): 348-353. Original Article The Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous
More informationDevelopment of teeth. 5.DM - Pedo
Development of teeth 5.DM - Pedo Tooth development process of continuous changes in predetermined order starts from dental lamina A band of ectodermal cells growing from the epithelium of the embryonic
More informationINSULIN ACTIVITY ON DENTAL PULP STEM CELL DIFFERENTIATION: AN IN VITRO STUDY
JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS Vol. 29, no. 3 (S1), 48-53 (2015) INSULIN ACTIVITY ON DENTAL PULP STEM CELL DIFFERENTIATION: AN IN VITRO STUDY D. LAURITANO 1, A. AVANTAGGIATO 2, V.
More informationDental Pulp Polyps Contain Stem Cells Comparable to the Normal Dental Pulps
Journal section: Oral Medicine and Pathology Publication Types: Research doi:10.4317/jced.51305 http://dx.doi.org/10.4317/jced.51305 Dental Pulp Polyps Contain Stem Cells Comparable to the Normal Dental
More informationBoon in Dentistry - Stem Cells
Review Article Boon in Dentistry - Stem Cells Pallavi Singh 1, Meghna Mehta 2, Pranav Thakur 3 1 MDS 3 rd Year Post Graduate Student in the Department of Public Health Dentistry, Babu Banarasi Das College
More informationSkeletal myogenic differentiation of human periodontal ligament stromal cells isolated from orthodontically extracted premolars
Original Article THE KOREAN JOURNAL of ORTHODONTICS pissn 2234-7518 eissn 2005-372X http://dx.doi.org/10.4041/kjod.2012.42.5.249 Skeletal myogenic differentiation of human periodontal ligament stromal
More informationIliac Crest: The Gold Standard
Iliac Crest: The Gold Standard Iliac crest is often considered the gold standard for harvesting. The iliac crest contains bone marrow which is a rich source of regenerative cells, including: Endothelial
More informationIsolation and Characterization of Multipotent Postnatal Stem Cells from the Alveolar Bone and Gingival Cervical Margin
Klinik für Zahnerhaltungskunde und Paradontologie im Universitätsklinikum Schleswig-Holstein-Campus Kiel an der Christian-Albrechts-Universität Kiel Deutschland Isolation and Characterization of Multipotent
More informationMesenchymal Stem Cells
Mesenchymal Stem Cells Science and therapeutic applications Dirk Büscher (Former VP-R&D Cellerix) GRIFOLS SA May 10 th, 2010 EMA 1 Discovery and Definition of Mesenchymal Stem Cells MSC must be plastic-adherent
More informationDental regenerative therapy: Stem cell transplantation and bioengineered tooth replacement
Japanese Dental Science Review (2008) 44, 70 75 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/jdsr MINI REVIEW Dental regenerative therapy: Stem cell transplantation and
More informationTHE USE OF DENTAL PULP STEM CELLS IN MEDICINE AND DENTISTRY
THE USE OF DENTAL PULP STEM CELLS IN MEDICINE AND DENTISTRY BY GLESNI HAF GUEST-ROWLANDS Pass with Merit RESEARCH PAPER BASED ON PATHOLOGY DAY LECTURES AT MEDLINK 2011 1 ABSTRACT Stem cell research is
More informationAbstract. 1. Introduction RESEARCH ARTICLE
RESEARCH ARTICLE JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE J Tissue Eng Regen Med 2016; 10: 475 485. Published online 16 August 2013 in Wiley Online Library (wileyonlinelibrary.com).1763
More informationSeznam produktů v rámci slevové akce East Port Praha/ Lonza PCM2014
Seznam produktů v rámci slevové akce East Port Praha/ Lonza Na uvedené produkty se vztahuje 30% sleva z kat. cen Na objednávce uveďte kód akce PCM2014 seznam: CC-3190 REGM BulletKit (CC-3191 & CC-4127)
More informationNeural Differentiation Potential of Stem Cells Derived from Dental Follicle and Periodontal Ligament stem cells
J. of Applied Tissue Engineering 2017; 4(1) Neural Differentiation Potential of Stem Cells Derived from Dental Follicle and Periodontal Ligament stem cells Naghmeh Bahrami 1, Gholamreza shirani 1*,Yadollah
More informationHuman dental pulp mesenchymal stem cells isolation and osteoblast differentiation
ORIGINAL ARTICLE Human dental pulp mesenchymal stem cells isolation and osteoblast differentiation Moustafa Alkhalil 1, Amer Smajilagić 1, Amira Redžić 2 1 Department for Oral and Cranio-Maxillo-Facial
More informationMeeting Report. From December 8 to 11, 2012 at Atlanta, GA, U.S.A
Meeting Report Affiliation Department of Transfusion Medicine and Cell Therapy Name Hisayuki Yao Name of the meeting Period and venue Type of your presentation Title of your presentation The 54 th Annual
More informationDental Stem Cells: Sources and Potential Applications
Curr Oral Health Rep (2014) 1:34 42 DOI 10.1007/s40496-014-0012-0 STEM-CELL BIOLOGY FOR TOOTH AND PERIODONTAL REGENERATION (M BARTOLD, SECTION EDITOR) Dental Stem Cells: Sources and Potential Applications
More informationKing s Research Portal
King s Research Portal DOI: 10.1053/j.sodo.2017.07.004 Document Version Peer reviewed version Link to publication record in King's Research Portal Citation for published version (APA): Sachdeva, S., &
More informationPulp biology update: Is regeneration possible or is it just pulp fiction?
Pulp biology update: Is regeneration possible or is it just pulp fiction? Hal Duncan, Division of Restorative Dentistry and Periodontology, Dublin Dental University Hospital, Trinity College Dublin, Ireland
More information06 Tooth Development and Eruption
+ 06 Tooth Development and Eruption Tooth development Root development PDL and alveolar bone development Primary tooth eruption and shedding Permanent tooth eruption Q. Where and how tooth starts to form?
More informationOdontogenic stem cells: biological characteristics and application in the dental tissue engineering
Odontogenic stem cells: biological characteristics and application in the dental tissue engineering Guo Ye 1, 2, Xuesong Yu 2, Lei Dou 2, Yan Wu 2, Yan Zhang 1 Chongqing Municipal Key Laboratory of Oral
More informationJournal Club WS 2012/13 Stefanie Nickl
Journal Club WS 2012/13 Stefanie Nickl Background Mesenchymal Stem Cells First isolation from bone marrow 30 ys ago Isolation from: spleen, heart, skeletal muscle, synovium, amniotic fluid, dental pulp,
More informationDental Morphology and Vocabulary
Dental Morphology and Vocabulary Palate Palate Palate 1 2 Hard Palate Rugae Hard Palate Palate Palate Soft Palate Palate Palate Soft Palate 4 Palate Hard Palate Soft Palate Maxillary Arch (Maxilla) (Uppers)
More informationNOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue
Genes & Diseases (2015) 2, 261e267 HOSTED BY Available online at www.sciencedirect.com ScienceDirect journal homepage: http://ees.elsevier.com/gendis/default.asp FULL LENGTH ARTICLE NOTCH3 is expressed
More informationKnowledge for Clinical Practice. A Guide for Dental Professionals
WWW.DENTALLEARNING.NET DENTAL LEARNING Knowledge for Clinical Practice A PEER-REVIEWED PUBLICATION Dental Stem Cells: A Guide for Dental Professionals Jeffrey Gruneich, PhD, MS; and Fiona M. Collins, BDS,
More informationAccelerate Your Research with Conversant Bio
Accelerate Your Research with Conversant Bio 400+ Participating MDs 50+ Partner sites for tissue procurement Continuous expansion of sourcing capabilities Closely monitored chain of custody Full regulatory
More informationDownloaded from Broadening the horizons of dentistry using stem cells. BROADENING THE HORIZONS OF DENTISTRY USING STEM CELLS
BROADENING THE HORIZONS OF DENTISTRY USING STEM CELLS Amol Manoj Karande 1, Cherish Simon Vergeese 2, Rakshit Khandeparkar 3. ISSN No. 2394-3971 Original Research Article 1. Oral and maxillofacial surgeon,
More informationPulp Regeneration with Stem Cells Ravi Kanth Chintala 1, C R Murali 2, Sundeep Franklin 3, Usha L Hirevenkanagoudar 4, G M Pranam 4, G A Manjunath 5
Received: 19 th January 2015 Accepted: 15 th March 2015 Conflicts of Interest: None Source of Support: Nil Original Research Pulp Regeneration with Stem Cells Ravi Kanth Chintala 1, C R Murali 2, Sundeep
More informationStem Cells and Sport Medicine
Stem Cells and Sport Medicine Rehal Abbas Bhojani, MD CAQSM Memorial Hermann Medical Group 2014 Sports Medicine Symposium of the Americas Stem cell biology Overview Potential applications of stem cells
More informationEquine DSC Therapy of Musculoskeletal Conditions: an Ultrasonographic Evaluation. Research Thesis
Equine DSC Therapy of Musculoskeletal Conditions: an Ultrasonographic Evaluation Research Thesis Presented in partial fulfillment of the requirements for graduation with research distinction in the undergraduate
More informationStem Cell Research: Applicability in Dentistry
Stem Cell Research: Applicability in Dentistry Shivani Mathur, MDS 1 /Rahul Chopra, MDS 2 /I. K. Pandit, BDS, MDS 3 / Nikhil Srivastava, BDS, MDS 4 /Neeraj Gugnani, BDS, MDS 4 In the face of extraordinary
More informationDental pulp stem cells: function, isolation and applications in regenerative medicine
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE J Tissue Eng Regen Med 2015; 9: 1205 1216. Published online 21 May 2014 in Wiley Online Library (wileyonlinelibrary.com).1899 REVIEW Dental pulp
More informationWhole Tooth Regenerative Therapy Using a Bioengineered Tooth Germ
Curr Oral Health Rep (2014) 1:43 49 DOI 10.1007/s40496-013-0004-5 STEM-CELL BIOLOGY FOR TOOTH AND PERIODONTAL REGENERATION (M BARTOLD, SECTION EDITOR) Whole Tooth Regenerative Therapy Using a Bioengineered
More informationHuman dental pulp stem/stromal cells (hdpscs) possess gene expression profiles
Isolation and Characterization of Human Dental Pulp Stem/Stromal Cells From Nonextracted Crown-fractured Teeth Requiring Root Canal Therapy Anderson Hsien-Cheng Huang, MS,* # Yuk-Kwan Chen, MS,* # Anthony
More informationGrowth kinetics and characterization of human dental pulp stem cells: Comparison between third molar and first premolar teeth
Journal section: Operative Dentistry and Endodontics Publication Types: Research doi:10.4317/jced.52824 http://dx.doi.org/10.4317/jced.52824 Growth kinetics and characterization of human dental pulp stem
More informationWe are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 4,000 116,000 120M Open access books available International authors and editors Downloads Our
More informationPREMATURE PRIMARY TOOTH LOSS
Disclaimer This movie is an educational resource only and should not be used to manage your dental health. All decisions about the management of premature primary tooth loss must be made in conjunction
More informationDental stem cells and their potential role in apexogenesis and apexification
doi:10.1111/j.1365-2591.2009.01622.x REVIEW Dental stem cells and their potential role in apexogenesis and apexification L. T. Friedlander, M. P. Cullinan & R. M. Love Sir John Walsh Research Institute,
More informationTOX 2017 Magyar Toxikológusok Társasága Tudományos konferencia Bükfürdő, Október
TOX 2017 Magyar Toxikológusok Társasága Tudományos konferencia Bükfürdő, Október 11-13. Histopathological evaluation of the teeth in regulatory studies: what are the needs and what can we learn from it
More informationBRITISH BIOMEDICAL BULLETIN
Journal Home Page www.bbbulletin.org BRITISH BIOMEDICAL BULLETIN Review Article Regeneration, Stepping into the Future of Dentistry Suneeth Shetty*and sunil tejaswi Dept of conservative dentistry & Endodontics,
More informationBone. Development. Tim Arnett. University College London. Department of Anatomy and Developmental Biology
Bone Development Tim Arnett Department of Anatomy and Developmental Biology University College London Bone development Outline Bone composition matrix + mineral Bone formation - intramembranous & endochondral
More informationCD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow
White Paper September 2016 CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow Lily C. Trajman, PhD Introduction: Hematopoietic Stem Cells (HSCs)
More informationBone Marrow Stroma in Myelodysplastic Syndromes
Bone Marrow Stroma in Myelodysplastic Syndromes Universidad de Salamanca Prof Mª M Consuelo del Cañizo Hematology Dept. University Hospital, Salamanca SPAIN Bone marrow stroma in MDS Introduction Mesenchymal
More informationIntroduction. Keywords: Pluripotency markers, Dental pulp stem cells, Thai, Primary teeth, Permanent teeth
Evaluation of pluripotency gene in Thai human dental pulp stem cells Yada Anantawat a, Kadkao Vongsavan a, Hathaitip Sritanaudomchai b*, Kemthong Mitrakul a, Taweepong Arayapisit c and Petcharat Kraivaphan
More informationRole of Dental Adult Stem Cells in Regenerative Medicine
JOFR 10.5005/jp-journals-10026-1077 REVIEW ARTICLE Ambika S Gupta, Siddharth Gupta, Sasidhar Singaraju, Medhini Singaraju ABSTRACT Statement of problem: Stem cell biology is today one of the most exciting
More informationTooth eruption and movement
Tooth eruption and movement Dr. Krisztián Nagy Diphydont dentition Deciduous dentition primary dentition Diphydont dentition Permanent dentition secondary dentition Mixed Dentition: Presence of both dentitions
More informationBlockade of TLR2 Inhibits P. gingivalis Suppression of Mineralized Matrix Formation by Human Dental Pulp Stem Cells
Blockade of TLR2 Inhibits P. gingivalis Suppression of Mineralized Matrix Formation by Human Dental Pulp Stem Cells By: Valerie Tom-Kun Yamagishi A thesis submitted in conformity with the requirements
More informationDISCOVERING ATCC IMMUNOLOGICAL CELLS - MODEL SYSTEMS TO STUDY THE IMMUNE AND CARDIOVASCULAR SYSTEMS
DISCOVERING ATCC IMMUNOLOGICAL CELLS - MODEL SYSTEMS TO STUDY THE IMMUNE AND CARDIOVASCULAR SYSTEMS James Clinton, Ph.D. Scientist, ATCC February 19, 2015 About ATCC Founded in 1925, ATCC is a non-profit
More informationThe health of teeth depends on the integrity of the hard tissue and the activity of the
Preliminary Study on Dental Pulp Stem Cell mediated Pulp Regeneration in Canine Immature Permanent Teeth Yuanyuan Wang, MD, Yuming Zhao, PhD, Weiqian Jia, MD, Jie Yang, MD, and Lihong Ge, PhD Abstract
More information저작권자로부터별도의허가를받으면이러한조건들은적용되지않습니다. 저작권법에따른이용자의권리는위의내용에의하여영향을받지않습니다.
저작자표시 - 변경금지 2.0 대한민국 이용자는아래의조건을따르는경우에한하여자유롭게 이저작물을복제, 배포, 전송, 전시, 공연및방송할수있습니다. 이저작물을영리목적으로이용할수있습니다. 다음과같은조건을따라야합니다 : 저작자표시. 귀하는원저작자를표시하여야합니다. 변경금지. 귀하는이저작물을개작, 변형또는가공할수없습니다. 귀하는, 이저작물의재이용이나배포의경우, 이저작물에적용된이용허락조건을명확하게나타내어야합니다.
More informationIndex. Adv Biochem Eng Biotechnol (2013) 130: DOI: / Ó Springer-Verlag Berlin Heidelberg 2013
Adv Biochem Eng Biotechnol (2013) 130: 305 309 DOI: 10.1007/978-3-642-37944-4 Ó Springer-Verlag Berlin Heidelberg 2013 Index A Acute lung injury (ALI), 105, 113 endotoxin-induced, 45 Acute respiratory
More informationSTEM CELLS. Dr Mohammad Ashfaq Konchwalla Consultant Orthopaedic Sports Surgeon
STEM CELLS Dr Mohammad Ashfaq Konchwalla Consultant Orthopaedic Sports Surgeon www.dubaisportssurgery.com PRACTICE SAUDI GERMAN HOSPITAL, DUBAI MEDCARE HOSPITAL, DUBAI Totipotent cells are cells that can
More informationThis electronic thesis or dissertation has been downloaded from the King s Research Portal at
This electronic thesis or dissertation has been downloaded from the King s Research Portal at https://kclpure.kcl.ac.uk/portal/ Thy-1(CD90) expression in dental pulp stem cells Abd-Elmotelb, Mona Assem
More informationDENTIN-PULP COMPLEX. Erlina Sih Mahanani. School of Dental sciences Universiti Sains Malaysia. Erlina Sih Mahanani
DENTIN-PULP COMPLEX School of Dental sciences Universiti Sains Malaysia Introduction Overview anatomy & histology of dentin and pulp. Development of dentin and pulp Structure of dentin and pulp Dentin
More informationStem Cell Primer. Hyun Bae, M.D. Professor of Surgery Department of Surgery Director of Education Cedars Spine Center
Stem Cell Primer Hyun Bae, M.D. Professor of Surgery Department of Surgery Director of Education Cedars Spine Center Perinatal Stem Cells Perinatal Stem Cells are defined as stem cells that are associated
More informationANATOMY OF THE PERIODONTIUM. Dr. Fatin Awartani
ANATOMY OF THE PERIODONTIUM Part II Cementum and Alveolar bone Associate Professor Periodontal division King Saud university Cementum Calcified mesenchymal tissue that forms the outer covering of the anatomic
More informationRegenerative endodontic treatment of teeth with incomplete root formation: a review
ISSN: Electronic version: 1984-5685 RSBO. 2016 Jul-Sep;13(3):199-208 Literature Review Article Regenerative endodontic treatment of teeth with incomplete root formation: a review Lincon Hideo Nomura 1
More informationDifferential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells
MOLECULAR MEDICINE REPORTS 16: 63-68, 2017 Differential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells PENGFEI BA 1 3, XIAOYU DUAN 4, GUO FU 3, SHUYAN
More informationThe Effect of White Mineral Trioxide Aggregate on Migration, Proliferation, and Odontoblastic Differentiation of Stem Cells from the Apical Papilla
University of Michigan Deep Blue deepblue.lib.umich.edu 213 The Effect of White Mineral Trioxide Aggregate on Migration, Proliferation, and Odontoblastic Differentiation of Stem Cells from the Apical Papilla
More informationSPACE MAINTAINER. Multimedia Health Education. Disclaimer
Disclaimer This movie is an educational resource only and should not be used to manage your health. All decisions about the management of premature loss of primary teeth and use of space maintainers must
More informationFundamental & Preventive Curvatures of Teeth and Tooth Development. Lecture Three Chapter 15 Continued; Chapter 6 (parts) Dr. Margaret L.
Fundamental & Preventive Curvatures of Teeth and Tooth Development Lecture Three Chapter 15 Continued; Chapter 6 (parts) Dr. Margaret L. Dennis Proximal contact areas Contact areas are on the mesial and
More informationHuman Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Introduction Kit Components Cat. # # of vials Reagent Quantity Storage
Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Catalog #5901 Introduction Human pluripotent stem cells (hpsc), including embryonic stem cells (ESC) and induced pluripotent stem
More informationMesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine
Mesenchymal Stem Cell-Mediated Functional Tooth Regeneration in Swine Wataru Sonoyama 1,3., Yi Liu 2., Dianji Fang 2, Takayoshi Yamaza 1, Byoung-Moo Seo 4, Chunmei Zhang 2, He Liu 5, Stan Gronthos 6, Cun-Yu
More informationHuman dental pulp stem cells: Applications in future regenerative medicine
Submit a Manuscript: http://www.wjgnet.com/esps/ Help Desk: http://www.wjgnet.com/esps/helpdesk.aspx DOI: 10.4252/wjsc.v7.i5.839 World J Stem Cells 2015 June 26; 7(5): 839-851 ISSN 1948-0210 (online) 2015
More informationCAP STAGE. Ans 1 The following are the stages of tooth development :
Ans 1 The following are the stages of tooth development : 1. Bud stage 2. Cap stage 3. Bell stage 4. Advanced bell stage 5. Formation of Hertwig s epithelial root sheath BUD STAGE 1. Around the eighth
More informationDental Mesenchymal Stem Cells Effect on Glioma Cells
Dental Mesenchymal Stem Cells Effect on Glioma Cells By candidates Oscar Hedlund, Paulina Tseng & Tutor Peyman Kelk Number of words in abstract: 163 Number of words in text: 2856 Number of figures: 4 Number
More informationEFFECTS OF DIFFERENT MICROENVIRONMENTAL CONDITIONS ON THE GROWTH AND DIFFERENTIATION OF DENTAL PULP STEM CELLS
Clemson University TigerPrints All Theses Theses 5-2012 EFFECTS OF DIFFERENT MICROENVIRONMENTAL CONDITIONS ON THE GROWTH AND DIFFERENTIATION OF DENTAL PULP STEM CELLS Matthew Cupelli Clemson University,
More informationMechanobiological Response of Stem Cells Derived from the Periodontal Ligament and Dental Pulp to Compressive Loading
University of Miami Scholarly Repository Open Access Theses Electronic Theses and Dissertations 3-7-5 Mechanobiological Response of Stem Cells Derived from the Periodontal Ligament and Dental Pulp to Compressive
More informationThe Role of IL-6 in Osteogenic and Neurogenic Differentiation Potentials of Dental Pulp Stem Cells (DPSCs)
University of Pennsylvania ScholarlyCommons Dental Theses Penn Dental Medicine Summer 6-30-2019 The Role of IL-6 in Osteogenic and Neurogenic Differentiation Potentials of Dental Pulp Stem Cells (DPSCs)
More informationCharacteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells
566 Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells SAKIKO FUJII 1, KATSUMI FUJIMOTO 2, NORIKO GOTO 3, MASAMI KANAWA 4, TAKESHI KAWAMOTO 2, HAIOU PAN 5, PETCHARIN SRIVATANAKUL
More informationStem cells: units of development and regeneration. Fernando D. Camargo Ph.D. Whitehead Fellow Whitehead Institute for Biomedical Research.
Stem cells: units of development and regeneration Fernando D. Camargo Ph.D. Whitehead Fellow Whitehead Institute for Biomedical Research Concepts 1. Embryonic vs. adult stem cells 2. Hematopoietic stem
More informationEffects of Sirtuin 1 on the proliferation and osteoblastic differentiation of periodontal ligament stem cells and stem cells from apical papilla
Effects of Sirtuin 1 on the proliferation and osteoblastic differentiation of periodontal ligament stem cells and stem cells from apical papilla Q.-B. Zhang 1,2 *, W. Cao 1 *, Y.-R. Liu 3, S.-M. Cui 2
More informationOsteoclast Culture Kit
K-ASSAY Osteoclast Culture Kit For the culture of Osteoclasts from precursor cells. Cat. No.: CC-107 Rat Osteoclast Precursor Cells, V-1 For Research Use Only. 1 Rev. 091708 K-ASSAY PRODUCT INFORMATION
More information