7/1/2014 FUNDAMENTALS OF SKIN GRAFTING No conflicts of interest in this talk.
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1 FUNDAMENTALS OF SKIN GRAFTING Superficial Anatomy and Cutaneous Surgery Course July 2014 David E. Kent, MD Clinical Instructor Division of Dermatology Georgia Health Sciences University Dermatologic Surgery Specialists, PC No conflicts of interest in this talk 1
2 Agenda Discuss different types of skin grafts Discuss physiologic processes in skin graft take Advantages, disadvantages, indications Surgical technique including bandaging and perioperative management Regional considerations of the body (e.g. lids) Instrumentation for split thickness skin grafting Management of perioperative problems Managing the suboptimal result TYPES OF SKIN GRAFTS AUTOGRAFT- A piece of skin taken from one location of one species and transplanted to a different location on that same species. (ftsg, stsg, composite graft) ALLOGRAFT-Skin from one species to a different member of the same species (cadaver graft) XENOGRAFT- Skin from one species placed on a different species (pigskin graft) 2
3 DEFINITIONS Full thickness skin graft (FTSG)- entire epidermis and dermis Split thickness skin graft (STSG)- entire thickness of epidermis and a variable thickness of dermis It is not uncommon to sculpt (thin) the thickness of a full thickness graft PHYSIOLOGY OF SKIN GRAFT TAKE Phase of serum imbibition(24-48hr) Phase of inosculation Phase of capillary ingrowth PHASE OF SERUM (PLASMIC) IMBIBITION Graft takes up wound exudate Graft becomes edematous, gains weight Fluid moves only one way Graft ph becomes acidotic within first hour Release of vasoactive substances Shift from aerobic to anaerobic metabolism Graft becomes fixed to wound bed by fibrin glue 3
4 PHASE OF GRAFT REVASCULARIZARION Inosculation-host endothelial buds hook up to existing arterioles and venules Ingrowth of host vessels into persisting endothelial channels Penetration of host vessels into graft dermis from host bed and margin Blood flow at 48 hours GRAFT REVASCULARIZATION-II Degree of vascularization depends upon vascularity of donor site, secondarily graft thickness GRAFT CONTRACTION Primary contraction- immediate shrinkage of graft once removed; recoil of elastic fibers Secondary contraction- once transferred to recipient site; stsg; ftsg 4
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6 LYMPHATIC FLOW Lymphatic flow returns by the 5-6 post op day Utilizes existing lymphatic channels REINNERVATION OF GRAFTS Sensory reinnervation is slow 2-3 months; may take years to complete Occurs first at margins, then moves inward FULL THICKNESS SKIN GRAFT INDICATIONS LOCAL FACTORS Defect size- local tissue not available Defect location & tissue mobility Tissue quality- radiated tissue, severe medical illness, heavy smoker 6
7 SKIN GRAFT INDICATIONS-II NON LOCAL FACTORS Aggressive tumor biology Uncertain margin control Risk of other methods is high (uncertain vascularity in area) Safe repair May result in a pleasing cosmetic result FULL THICKNESS SKIN GRAFT ADVANTAGES Less tendency to contract Less tendency to develop a smooth sheen and resist cosmetics Less tendency to pigment post op Better chance to have texture and thickness match Durability Appearance improves with time No special instrumentation required FULL THICKNESS SKIN GRAFTS DISADVANTAGES Adequate recipient bed-clean (+) excellent vascular bed required Meticulous technique-edge approximation and hemostasis Donor area requires closure (linear, flap) Potential complications at two sites Texture, color, and surface differences between graft and adjacent tissue 7
8 FULL THICKNESS SKIN GRAFTS DONOR SITES Retro auricular Pre auricular Upper lid Neck Upper inner arm Clavicular Groin Nasolabial crease MATCHING DONOR SKIN TO REDCEPIENT SITE Total size including depth; use gentian violet and template Consider skin thickness Sebaceous and actinic quality FULL THICKNESS SKIN GRAFT SURGICAL TECHNIQUE 1-Donor site selection 2-Appropriate marking and sizing, tailor defect size 3-Harvest skin graft ( safe keeping) 4-Repair donor defect 5-Defat graft (keep moist) 6-Prepare recipient bed (hemostasis, delayed graft- freshen edges) 7-Placement of graft 8-Meteculous approximation of edges- tacking sutures at edge, basting sutures, running at periphery 9-Dressing 10- Pt. Education: diet, activity, any local care, f/u 8
9 SKIN GRAFT SURGICAL TECHNIQUE-II 11- Follow up visit: bolster / dressing removal 12- Discussion of graft appearance and demonstration of local care 9
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11 SILK HAS EXCELLENT KNOT STABILITY 11
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17 Post op day 7 Post op week 2 17
18 3 Months post op CLEANING GRAFT EDGES 18
19 ROLL CENTER --OUT 19
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32 FTSG Upper Eyelid From Upper Lid Donor FTSG Upper Eyelid From Upper Lid Donor FTSG Upper Eyelid From Upper Lid Donor 32
33 FTSG Upper Eyelid From Upper Lid Donor TRACTION SUTURES TRACTION SUTURES Access & Visualization 33
34 BURROWS GRAFTS 34
35 POST OP DAY 7 35
36 36
37 37
38 BOLSTER REMOVAL 38
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40 3 WEEKS POST OP 40
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42 3 MONTHS POST OP 3 MONTHS POST OP 42
43 DELAYED 2 WEEKS 43
44 DELAYED 2 WEEKS 44
45 BASTING SUTURES 45
46 46
47 DRESSING OVER GRAFT Goal: to prevent external mechanical forces from disturbing physiologic take of graft to recipient bed Bolster dressing tie over vs- non tie over dressing protects graft PERIOPERTIVE MANAGEMENT Written instructions Telephone numbers for questions/problems Acceptable diet Acceptable activity, bathing Rx- Antibiotics; pain medications Follow up depends on distance traveled Stress their actions determine survival of graft and outcome Bolster off at about 7 days Review graft appearance: color, contour, expectations- mirror in patient s hand PERIOPERATIVE MANAGEMENT-II Follow up- depends on graft appearance Do not abandon patient if outcome appears unfavorable rather opposite 47
48 SPLIT THICKNESS GRAFTS CLASSIFICATON OF SPLIT GRAFTS Thin inch Intermediate inch Thick inch 48
49 STSG DONOR SITES Thigh Buttock Inner arm Abdomen Be mindful in young female pt. (swim suit) SPLIT THICKNESS GRAFT INDICATIONS LOCAL FACTORS Large size Compromised recipient bed-radiated tissue, questionable vascular supply NON LOCAL FACTORS Coverage of wound before formal reconstruction with flaps (delayed, tissue expansion) Observation of wound after removal of tumor with aggressive/ recurrent disease Biologic Band-Aid 49
50 SPLIT THICKNESS FRAFTS ADVANTAGES High rate of success Simple to apply; less meticulous technique required Rapid coverage of larger defects Ample donor sites May use with marginal recipient bed Durability SPLT THICKNESS GRAFTS DISADVANTAGES Appearance- color and texture match Special instrumentation required for larger defects Graft contraction Appearance worse over time SPLIT THICKNESS GRAFT SURGICAL TECHNIQUE 1-Donor site selection 2-Appropriate sizing-depth +dimensions 3-Prepare donor site; mineral oil 4-Stabilizaton of donor site; harvest; safe placement of graft until ready 5-Secure graft 6-Fenestraton of graft vs.- meshed graft 7-Dressings for graft and donor site 8-Written instructions 50
51 INSTRUMENTATION A. Freehand harvest 1. Blue blade 2. Weck knife 3. Hamby knife B. Electric dermatome 1. Power Padgett dermatome 2. Brown dermatome 3. Davol-Simons C. Power Dermatome 1. Zimmer dermatome 51
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54 ZIMMER DERMATOME 54
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57 3 WEEKS POST OP 57
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63 63
64 SCC IN FIELD OF PSORIASIS SCC IN FIELD OF PSORIASIS AFTER NOT USING TOPICALS TO HELP PSORIASIS DEFECT PRE STSG 64
65 POST OP DAY 21; 3 PIECES STITCHED RECURRENT BCC S/P RT RECURRENT BCC S/P RT 65
66 MELANOMA WITH LARGE AMELANOTIC COMPONENT READY FOR GRAFTING- 16x14 cm FRESHENING BASE & EDGES 66
67 TUMESCENT ANESTHESIA FOR LARGER AREAS HELPFUL: % LIDOCAINE DEPENDING ON SIZE Tumescent Anesthesia Tumescent Anesthesia 25g Spinal needle PIECES DRAPED OVER DEFECT DONOR SITES WITH GRAFTS HARVESTED 67
68 GRAFTS SECURED AT PERIPHERY AND OVERLAPPING 4.0 Vicryl Rapide TRIM AWAY EXCESS GRAFTS SECURED TOGETHER WITH BITES INTO WOUND TO SECURE AND #11 BLADE TO PIE CRUST GRAFT 68
69 BASTING SUTURES RUNNING LOCK WITH TACKING 2-0 SILKS FOR TIE OVER BOLSTER DRESSING BOLSTER CONSTRUCTED OUT OF KERLIX FLUFF COVERED WITH 4x4 GAUZES AND COVER ROLL TAPE ARM PLACED IN SLING UNTIL F/U BOLSTER REMOVAL 69
70 BOLSTER REMOVAL POST OP WEEK 1 Small seroma drained FOLLOW UP Post op week 12 Post op week 2 Post op week 12 70
71 SPLIT THICKNESS GRAFTING Pre op Post op week 12 AMELANOTIC MELANOMA; S/P MOHS WITH PERM. SECTIONS- 14X16 CM; 8 MONTHS POST OP AMELANOTIC MELANOMA PT.--DONOR SITS OF STSG WITH AREAS OF HYPERTROPHY; 8 MONTHS POST OP 71
72 Hypertrophic Scarring of Donor Site Improved Following Multiple Laser Treatments Hypertrophic Scarring of Donor Site Improved Following Multiple Laser Treatments STSG 3 Years Out
73 STSG 3 Years Out PINCH GRAFTS 73
74 COMPLICATIONS AND MANAGEMENT 1- Bleeding Must see true bleed vs- ointment mix Inspect graft; if active bleeding, take down and effect hemostasis Rebandage; if > hours, graft may not be viableconsider additional graft Prevention- meticulous technique, thrombin, delayed graft, nicks in graft, review pre op check list 2- Infection rare but can occur COMPLICATIONS AND MANAGEMENT-II 3-Incomplete take contour irregularity 4-Free edge contraction 5-Hypertrophy HYPERTROPHY OF GRAFT 74
75 APPEARANCE HYPERTROPHY OF GRAFT 75
76 FREE EDGE CONTRACTURE KELOID OF DONOR SITE 76
77 MELANOMA WLE AND STSG; MRSA WITH AREA OF NECROSIS 14 X 12 CM PIECES STITCHED EXUBERANT GRANNULATION NECROSIS SITES SMOKING S EFFECT ON RANDOM PATTERN FLAP 77
78 NECROSIS OF GRAFT Do not aggressively debride graft Do frequent dressing changes Do see pt. frequently Reevaluate once demarcation of necrosis complete to go over options CAUSES OF GRAFT FAILURE Poor planning Poor technique Inadequate hemostasis, necrotic debris in recipient bed, inadequate vascular bed Infection Patient indifference to instructions 78
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80 8 Month F/U 80
81 THANK YOU! 81
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