Antisense mrna for NPY-Y 1 receptor in the medial preoptic area increases prolactin secretion
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1 Brazilian Journal of Medical and Biological Research (1999) 32: NPY-Y 1 receptors in the MPOA and PRL secretion ISSN X 1161 Antisense mrna for NPY-Y 1 receptor in the medial preoptic area increases prolactin secretion N.A. Silveira 1 and C.R. Franci 2 1 Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade de Goiás, Goiânia, GO, Brasil 2 Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil Correspondence C.R. Franci Departamento de Fisiologia Faculdade de Medicina de Ribeirão Preto, USP Av. Bandeirantes, Ribeirão Preto, SP Brasil Fax: crfranci@fmrp.usp.br Research supported by CAPES, CNPq and FAPESP. Received December 30, 1998 Accepted June 18, 1999 Abstract Key words Neuropeptide Y Medial preoptic area Neuropeptide Y-Y 1 receptor Prolactin mrna for neuropeptide Y-Y 1 receptor Luteinizing hormone Follicle-stimulating hormone Introduction
2 1162 N.A. Silveira and C.R. Franci Material and Methods Results and Discussion
3 NPY-Y 1 receptors in the MPOA and PRL secretion 1163 ng/ml LH Sense FSH Antisense * PRL Figure 1 - Effect of four microinjections of sense or antisense mrna oligonucleotides (250 ng) for neuropeptide Y-Y 1 receptors into the medial preoptic area at 12-h intervals on luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin (PRL) secretion in ovariectomized rats treated with estrogen (50 µg) and progesterone (25 mg). Values are reported as means ± SEM for 8 animals. *P<0.02 vs sense group (control).
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