Differential effects of isoproterenol on the activity of angiotensin-converting enzyme in the rat heart and aorta

Transcription

1 Brazilian Journal of Medical and Biological Research (1999) 32: Isoproterenol and angiotensin-converting enzyme activity ISSN X Short Communication 355 Differential effects of isoproterenol on the activity of angiotensin-converting enzyme in the rat heart and aorta V.C.W. Busatto 1, V. Cunha 2, M.A. Cicilini 2 and J.G. Mill 2 Departamento de 1 Biologia and 2 Ciências Fisiológicas, Universidade Federal do Espírito Santo, Vitória, ES, Brasil Correspondence J.G. Mill Departamento de Ciências Fisiológicas, UFES Av. Marechal Campos, Vitória, ES Brasil Fax: Research supported by CNPq (No /95-6) and FINEP (No ). Received May 19, 1998 Accepted November 19, 1998 Abstract Key words Angiotensin-converting enzyme Isoproterenol Cardiac hypertrophy Myocardial infarction Heart failure

2 356 V.C.W. Busatto et al.

3 Isoproterenol and angiotensin-converting enzyme activity 357 Table 1 - Morphologic and hemodynamic data for the control and isoproterenol-treated rats. Data are reported as means ± SEM. LV, Left ventricle; RV, right ventricle. *P<0.05 vs control group (Student t-test). Control group (N = 7) Isoproterenol group (N = 8) Body weight (g) 291 ± ± 18 RV weight (mg) 162 ± ± 16* LV weight (mg) 632 ± ± 48* Heart rate (bpm) 323 ± ± 20 Mean blood pressure (mmhg) 80 ± 6 75 ± 6 LV systolic pressure (mmhg) 98 ± 7 92 ± 5 LV end-diastolic pressure (mmhg) 6 ± ± 0.3 RV systolic pressure (mmhg) 25 ± 1 33 ± 3* RV end-diastolic pressure (mmhg) 5 ± ± 0.4

4 358 V.C.W. Busatto et al. Figure 1 - Angiotensin-converting enzyme (ACE) activity in the homogenates of the right atrium (RA), left atrium (LA), right ventricle (RV) and left ventricle (LV) of control rats (black bars, N = 7) and isoproterenol-treated rats (gray bars, N = 8). *P<0.05 vs control group (Student t-test). ACE activity (nmol His-Leu g -1 min -1 ) RA LA RV LV * *

5 Isoproterenol and angiotensin-converting enzyme activity 359 ACE activity (nmol His-Leu g -1 min -1 ) Plasma Acknowledgments ACE activity (nmol His-Leu g -1 min -1 ) * Aorta Figure 2 - Angiotensin-converting enzyme (ACE) activity measured in plasma and in aorta homogenates of control rats (black bars, N = 7) and isoproterenoltreated rats (gray bars, N = 8). *P<0.05 vs control group (Student t-test). References 1. Peach S (1977). Renin-angiotensin system: biochemistry and mechanisms of action. Physiological Reviews, 57: Campbell DJ (1987). Circulating and tissue angiotensin systems. Journal of Clinical Investigation, 79: Unger T, Ganten D & Lang RE (1986). Tissue converting enzyme and cardiovascular actions of converting enzyme inhibitors. Journal of Cardiovascular Pharmacology, 8: S75-S Lindpaintner K, Lu W, Niedermajer N, Schieffer B, Just H, Ganten D & Drexler H (1993). Selective activation of cardiac angiotensinogen gene expression in postinfarction ventricular remodeling in the rat. Journal of Molecular and Cellular Cardiology, 25: Nio Y, Matsubara H, Murasawa S, Kanasaki M & Inada M (1995). Regulation of gene transcription of angiotensin II receptor subtypes in myocardial infarction. Journal of Clinical Investigation, 95: Hirsch AT, Talness CE, Heribert S, Paul M & Dzau VJ (1991). Tissue-specific activation of cardiac angiotensin converting enzyme in the experimental heart failure. Circulation Research, 69: Hokimoto S, Yasue H, Fujimoto K, Sakata R & Miyamoto E (1995). Increased angiotensin converting enzyme activity in left ventricular aneurysms of patients after myocardial infarction. Cardiovascular Research, 29: Busatto VCW, Cicilini MA & Mill JG (1997). Increased angiotensin-converting enzyme activity in the left ventricle after infarction. Brazilian Journal of Medical and Biological Research, 30: Nagano M & Ogihara T (1994). Role of the cardiac renin-angiotensin system in left ventricular hypertrophy. In: Lindpaintner K & Ganten D (Editors), The Cardiac Re-

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