te cs#^; J\ ;sar-i t*rr rk, 6;t-g3l; cft9 c CSJ:<.I q-l: AQ! ::kll t. ' J J;J &i Jt-,,r-.r:ar<i-aT.r.2r.*t1.1,.lJ+]1s 6*1t r ;.ri*yjl.-.

Transcription

1 ,j1.1s c/lt.r,rul4. crl.r;, F:; 1sJ. ot(:,i.r cc,rjl"t fsbs 6 Jl--'J)l.l o.r('1"'l.l,,ii.,-, J L+t1( 6ln33lr 43li-,i^'i 5-r- 6;t-g3l; cft9 c CSJ:<.I q-l: AQ! :rjljis, ::kll t. ' J J;J &i Jt-,,r-.r:ar<i-aT.r.2r.*t1.1,.lJ+]1s 6*1t r ;.ri*yjl.-.;c (Trigonellafoenum-'graecum L") 4l,l,:; +,1.r.r,l.r,t':.,.1 o;t,.,,11 : t -r-rj: te cs#^; J\ :L^itlJ gl.lli.,.l cs-rt, '"1 6.:a-" :l(.1 ;sar-i t*rr rk, 1Yo :+-1,.,LQ ort ^.1. \ Y11 ;t:,..lli

2 Kerman ljniversity of Medical Sciences Faculty of Pharmacy Herbal and Traditional Medicines Research Center Pharm. D Thesis Title: F ormulation and physicochemical characteri zation of antinociceptive and anti inflammatory of oral sustained release tablet of standardized extract of Fenugreek seeds (Tr i gonel I a fo enum- gr a e c um L.) By, Balal Rahbari Magham Supervisors: Dr Mehdi Ansari Dr Fariba Sharififar Summer2017 Thesis No: 925

3 -r-:ls.j-<?.+5. -t_l*;-'u J-L-l -( :;: :tali,., (ur-.:*-* 6G.f-- il u;l,r-! :-=-1 "l! L, - -jj! ---*- )l uju -J.rl :;>.JS,s^.(.r-; :\,:-.,:;:.ggojrj,-,1l, C^le,! :;: 51;:l..a*1 -j,-fl J -s;l(,,;:l:-.--isi J:.-.J- -51^-;jL: )s; *. ;,:- tl;t.-lt- j! J). )* t J+,t 3t )W, r;f ulq r;".:l3o 6\i: u'li: JL^15 ^S c-q;i:\.r-y- u# rflrl s obr,;q,.-,.l-: Trigonella --L f [!.!+, "6.t_5 o:li:*l ;L-.gLay;l: gt= "1 L"ai ;l,tl;.r" -r::*-a 61;1: +5 -t*;!.:,. -, ja.-r",g;*,4l,[.ar;.-)u.lar'rl<i 61.\: Jbenum - gyaecum L. o-li -(-rj "rl<s-.\ -Slrf -^-,*i -,o; ;;J.-r-!.f :kjl -u; 3 :;: J-) -f\i - G ii,. "j\g +(.-r:-( :Q*rl ;L, "j! -<_ ;: l; *L c4::l: c-tl;.-< *\y u" +!!; +ll:.) J ; LC: j_l -.L_t, _"-L jl il_-: L -(i,l,"-l; -1r.,i,15^Ie f lj +_L- _,1 -r- 3 ojj ^"g _,i;! ;l a1l*: ol; -(-:j a,l: :',t5.-ij;,,, I r r : r r.r-r.j--t j-<--l jl.i-: 3-So,Los J-.;-I,.-L j:) 4. a-.r A' Jr[t L -o c:l: )::e,-:u _2 J,_r; o:li-l og o;l-s.t:rs :-,1Ik-.1 5lr. ;\;; o.:l erly.:-c!.!!:*.,.1: _&1 {;L< GG-pLtr -r-v;._-(.1-t tl-t^a; Lr)J.-tj-i-.!d -:*f,-,-."-\;i -( :-:),-. Lr_-_-:

4 II e.-)g.+<o)l- jlli" ;,rj,,j--.l;s C^; 'P e+;4,1n.,.,rtr o;j ;:-\-(-- *-j JL: o-l.: qj i:.5 if-l G_r-J -(-,1{L- : -sijj--.;rl:rri..;-* *.el ;) e:;s,y l:, \ "':S- L.5 :l: -"i..j-: J_1-\-u; -,1 "-r.i **-r" n-.e:6-l: G-ef jy 3=11-.4i,.".:*-r, J3LL -.--;f-* jvf; \ e-gu-;,o::-.):,1.r:;-;(!".)-: Y.ff,r*:;lI[:-l.-:l-^;i Le)) J \Y,.1 :;1ri[:--1 ;t ;t! f-j.rrir, otv ai-))a;:.ji J-t- L.Y *;:rl-t';[-l.rlfl J--p:) ] ',iy:;lri[:*.l.--rlfl L t,1v G.ro,3 r s:\, -<;L:l;l.-t" '.Y\f,'ll o'1*,.glasoi )-;s,,:.rt5 Jo-: t:grf;.r.:.-;t -,.r ;: +al 1 o;-e: J* -,, LtJlr:L, :-;.;); j:f a-i*i: 0.. it j * t..*u" I-,; -(:! Ara-; riri,.!.!l-r- s fr,-s-;j ttf ;L o-.:, L, osi s t--,j1-=;_.,.,l: l^li; a*, -.ir<jl- oz:) L.,-r;,1 qr<l: )) ) J-?)) 1i,.1 -!.*-.gl:;:.j-e)) qt,ai JLz=;-.gL:;:. -r."i c--1, -L^a;-:1 Y,11 i): :\ b.r-,i,i-;j L. Lt). )j$a-l.-ilr.-,l : ;-av e-tr q.2-j ti :6f ^.,^l 3 i-cr. -ta;:.j;l.rr J3! "l.g t: 6,:-i ;;* x;lu: _s\::fi..r:,jlu ;;\*a- J..-L.r.::\, J>t-:l ;L;..--,1,:1 c--l'":3 r\,/ -1..,,r -(: y-) rgrft dj3.,*.:l-- iji ) L^.J-l -^r..9l: a-;:.;-l,.,;-.nr^^-i o:;ji:.;,-,l- o( -t"i,----t, c---cl ly,r.$..ro'rl:jl-, ur;1-)-..; S-,1-L-! 6 i J-4; r*a;:t' q-.)! Ar1 ; "-r:l;! t^-c': *.(;; :;l.-lj :-.l o;loo.-;rlor^*^i,r- ".-+-.-; 1i1-* :rl.t-;,--=,\--j :.5-rS.- ;..!+- a-il: o-u

5 III.r--.Lal.-(- Abstract Introduction: Pain is an unpleasant fbeling and emotional experience associated with actual or potential tissue damage. It is often caused by actual or potential tissue damage resulted from chemical, thermal, mechanical and electrical stimuli. Pain is felt due to the activation of nociceptors after a direct noxious stimulation or release of pain mediators from damaged tissues to spinal cord and then to higher centers via the dorsal hom. Inflammation is actually natural body response to recovery from injury, which allows the body to heal; however, chronic inflammation may result in serious diseases, such as arthritis, diabetes, obesity, cancer and cardiac problems. Since herbal medicines have fewer side effects, they can be used instead of synthetic drugs. Fenugreek (Trigonella foenum-graecum) contains alkaloids, flavonoids, saponins, mucilage, etc., which have analgesic and anti-inflammatory properties. The fabrication of sustained release tablets from dry extraction of fenugreek seeds provides good drug concentration in a certain time, along with some other benefits. Methodology: Dry fenugreek seeds were supplied from market. A{ter confirmation of its scientific name, they were milled, passed through a sieve of a certain mesh size, and extracted in the presence of ethanol 80olo, using maceration method. The tablets were prepared with wet granulation technique. Physiochemical properties of the tablets were examined, using the weight uniformity test, hardness test, and corrosion test. In vitro release and stability of the tablets were also evaluated. Results: Results from the premier formulation showed that given the employed method in this formulation, the prepared tablets had desirable physiochemical properties. The mean tablet weight was 517 milligrams with the standard deviation of and relative deviation of 2.33, and the mean tablet hardness 4.67 with the standard deviation of 0.22 and relative deviation of 4.7 percent. Study of tablet friability indicated that percentage weight loss of the sample tablets was Drug release exhibited a suitable trend and took place within 500 minutes. The stability test was carried out over a six-month period under three temperature conditions in refrigerator, environment, and oven (40"C)- The minimum amounts of trigonelline in the refrigerator, environment, and oven (40"C) conditions were yo, yo, and 92.69%o, res pectivel y. Discussion and Conclusion: Considering the results related to the means and standard deviations related to tablet weight, they enjoyed desirably suitable and uniform weighl Tablet hardness was also acceptable. Percentage friability of the tablets was less than one percent, which was satisfactory. The tablets dissolved within 8-12 hours, which is suitable for slow release products. The remaining Trigonelline at the refrigerator, ambient, and oven temperatures exceeded 90 percent, which indicated formulation stability- Keywords: Formulation, Physiochemical Properties, Sustained Release Table! Stand. ardt:zed Fenugreek Seed Dry Extract