Phytophthora cactorum

Transcription

1 Phytophthora cactorum A symptom of Phytophthora rot caused by Phytophthora cactorum on strawberry petioles and stolons NAO Masahiro Glomerella cingulata Phytophthora cactorum /kg Phytophthora nicotianae van Breda de Haan Phytophthora cactorumlebert CohnSchröter NARM mg/L 255 CMA BBLTM Corn Meal Agar,17g/L, Becton, Dickinson and Company 2 A-1 MAFF A-2 MAFF A-1 A-2 PSA 18g/L mm PSA

2 Phytophthora cactorum 2 PSA A-1 A-2 V8 V8 100, Campbell soup company 163ml 2g 1L 18g/L V A-1 A-2 PD MagExtractor -Plant Genome-TOYOBO DNA P. nicotianae E-1 E-2 DNA PCR TaKaRa ETaq 0.1l10ETaq Buffer 2ldNTPMix 1.6l 20pmol/l 0.5l DNA 2lDNA 50l 20l PCR I Cycer JA PCR 10l loading buffer 4l 1.5S 1200g/cm DNA Martin et al.2004fmph-8b 5'-aaaagagaaggtgttttttatgga-3'FMPh-10b 5'-gcaaaagcactaaaaattaaatataa-3'PCR 457bp PCR White et al.1990internal transcribed spacersits ITS1 5'-tccgtaggtgaacctgcgg-3'ITS4 5'-tcctccgcttattgatatgc-3' ITS1 ITS2rDNA PCR PCR ITS1 ITS4 5'3' BLASTBasic Local Alignment Search Tool GenBank/EMBL/DDBJ A-1 V /ml kKG-106SHLD mol/m 2 S20,000lux mm A-1 26mmA-2 23mm

3 P. cactorum Waterhouse and Waterston1966 V A mA m l/b A mA m A mA m A ma m P. cactorum P. cactorum 457bp Phytophthora 2 ITS1 ITS2 rdna A A A-1 P. cactorumaccession GU A-2 P. cactorum accessioneu bp

4 Phytophthora cactorum

5 Senga SenganaKorona P. cactorum Eikemo et al.2000 P. cactorum leather rot Rose Seemüller 1984Santos et al., P. nicotianae Suzui et al.1980 P. cactorum Waterhouse1963P. cactorum PCR Martin et al.2004 ITS rdna Waterhouse and Waterston1966P. cactorum Grove et al.1985 Rose1926 Wright et al.1966 Madden et al.1991p. cactorum crown rotdeutschmann Madden et al mm Deutschmann, von F.1954 Eine Wurzelfäule an Erdbeeren, hervorgerufen durch Phytophthora cactorum (Leb. et Cohn) Schroet., Nachrichtenblatt des Deutschen Pflanzenschutzdienst Eikemo, H., Stensvand, A. and Tronsmo, A.M.2000 Evaluation of methods of screening strawberry cultivars for resistance to crown rot caused by Phytophthora cactorumannals of applied biology

6 Phytophthora cactorum Grove,G.G., Madden,L.V., and Ellis, M.A.1985 Influence of temperature and wetness duration on sporulation of Phytophthora cactorum on infected strawberry fruitpytopathology ,2- / Phytophthora cactorum Phytophthora nicotianae Phytophthora cactorum Madden,L.V., Ellis,M.A., Grove,G.G., Reynolds, K.M. and Wilson, L.L.1991Epidemiology and control of leather rot of strawberriesplant disease Martin,F.N., Tooley,P.W.and Blomquist, C.2004Molecular detection of Phytophthora ramorum, the causal agent of sudden oak death in California, and two additional species commonly recovered from diseased plant materialphytopathology Phytophthora cactorum Phytophthora cactorum Rose,D.H Leather rot of strawberries Journal of agricultural research Rose,D.H.1926 Relation of strawberry fruit rots to weather conditions in the fieldphytopathology Santos, B. de los, Porras, M., Blanco, C., Barrau, C. and Romero, F First report of Phytophthora cactorum on strawberry plants in SpainPlant disease Seemüller, E.1984Crown rot (vascular collapse), Maas, J.L.(ed), Compendium of strawberry diseases, APS PRESSSt. Paul Phytophthora sp Suzui, T., Makino, T. and Ogoshi, A.1980 Phytophthora rot of strawberry caused by Phytophthora nicotianae var. parasitica in Shizuoka Annals of the phytopathological society of Japan Waterhouse,G.M.1963 Key to the species of Phytophthora de BaryMycological papers Waterhouse,G.M. and Waterston,J.M.1966PHYTOPHORA CACTORUMC.M.I. Descriptions of Pathogenic Fungi and Bacteria No.111 White,T.J., Bruns,T., Lee,S., and Taylor, J.1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics PCR Protocols:a guide to methods and applications Innis,M.A., Gelfand, D.H., Sninsky,J.J.and White,T.J. ed. Academic PressSan Diego Wright,W.R., Beraha,L. and Smith,M.A.1966 Leather rot on California strawberriesplant disease reporter

7 Abstruct In June 2011a disease characterized by cavernous black spots was observed on strawberry petioles and stolons in a open field in Ehime PrefectureJapan. The symptom was very similar to the typical symptom of strawberry anthracnose caused by Glomerella cingulata According to microscopic observationzoosporange and aseptate mycelia showing the typical characteristics of Phytophthora rot formed on the lesionsthe causal agent was identified as Phytophthora cactorum on the basis of the morphological characters of the zoosporange and sexorgans on culture media, compared with the previous reports. The morphological identification was supported by diagnostic PCR using primers specific to Phytophthora spp. or the DNA sequence of the rdna ITS1 and ITS2 regions of isolates had more than 99.6% homology to P. cactorum. The pathogen was inoculated onto 3 cultivars Amaotome, Sagahonokaand Sachinokaas potted plant. Four days after inoculation, using the zoospore suspension, the characteristic symptoms were reproduced on the petiolesonly cultivar Amaotome has maintained the same symptom 30 days after inoculation. On the other hand, inoculation of the oospore suspension, only cultivar Sagahonokashowed the symptom of wilt

8 Phytophthora cactorum P.cactorum 2A-110m 3A-1 4A-1 10m 5A-14 6A A