Curriculum Vitae. 1) Personal Information: Gender: Female. Nationality: Iranian

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1 1 Curriculum Vitae 1) Personal Information: Surname: TAVALAEE First name: MARZIYEH Gender: Female Nationality: Iranian Date of birth: year: 1978 month: 7 day: 28 = Year: 1357 month: 5 day: 6 Place of Birth: Esfahan Occupation: Ph.D Academic member of the in Tehran, Iran Address: Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, for Biotechnology, ACECR, Isfahan, Iran P.O. Box: , Tehran, IRAN. TEL: FAX: tavalaee.royan@gmail.com; tavalaee.m@royaninstitute.org

2 2 Activities International Articles National Research & Scientific Articles Book publications: - Persian - Collaborated in writing English book chapter - writing English book chapter Conferences and Meetings Projects Prize Presentation of Workshop Consulting of thesis Patent Number

3 3 2) Educational background: Degree Major University Date B.Sc M.Sc Biology Animal Sciences Biology Falavarjan Azad Uni. (Esfahan, Iran) Damghan Azad Uni. (Damghan, Iran) Ph.D Developmental biology Tehran Azad Uni Research Sciences (Tehran, Iran)

4 4 3) Book publications: 1- Sperm: Identification and selection of sperm from Sperm the biological and clinical aspect in ICSI patients (2010), is written by Dr.MH.Nasr- Esfahani and Marziyeh Tavalaee. ISBN: Second edition 2- WHO laboratory manual for the Examination and processing of human semen, Translated by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee (ISBN: ). 3- Collaborated in writing a English book chapter: Mechanism of human oocyte activation during ICSI and methodology of overcoming low or failed fertilization. Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, 1 DOI / _1, Springer Science+Business Media, LLC Writing an English book chapter: Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay. is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. Spermatogenesis: Methods and Protocols, Methods in Molecular Biology, vol. 927, DOI / _24, Springer Science+Business Media, LLC An English book chapter Sperm selection based on surface electrical charge is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. Non-Invasive Sperm Selection for In Vitro Fertilization: Novel Concepts and Methods. DOI / _4 Springer Science+Business Media, New York In Vitro Fertilization (2013), is written by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN: Intra-Cytoplasmic Sperm Injection (2014) by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN: Cellular and molecular aspects of reproduction biology (2015) by Dr.MH.Nasr-Esfahani and Marziyeh Tavalaee. ISBN:

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6 6 4) Articles published in International Journals: Panahi S, Fahami F, Deemeh MR, Tavalaee M, Gourabi H, Nasr-Esfahani MH. Chances to Have A Boy after Gender Selection by Pre-Implantation Genetic Diagnosis Are Reduced in Couples with only Girls and without A Boy Sired by The Male Partner. Int J Fertil Steril Jan-Mar; 10(4): Tavalaee M, Kiani Esfahani A, Nasr-Esfahani MH. Relationship between potential sperm factors involved in oocyte activation and sperm DNA fragmentation with ICSI clinical outcomes. CELL JOURNAL (Yakhteh) 2017 Jan-Mar; 18(4): Nasr-Esfahani MH, Deemeh.MR, Tavalaee.M, Sekhavati MH, Gourabi H. Zeta sperm selection improves pregnancy rate and alters sex ratio in male factor infertility patients: a double-blind, randomized clinical trial. Int J Fertil Steril Jul-Sep; 10(2): Torki B, Tavalaee M, Bahadorani. M, Nasr-Esfahani MH. Selection of physiological sperm during ICSI. Andrologia Apr; Modarres P, Tanhaei S, Tavalaee.M, Ghaedi. K, Deemeh.MR, Nasr-Esfahani.MH. Assessment of DPY19L2 deletion in familial and non-familial individuals with globozoospermia and DPY19L2 genotyping. Int J Fertil Steril Jul-Sep; 10(2): Tavalaee M, Nasr-Esfahani MH. Expression profile of PLCζ, PAWP and TR-KIT in association with fertilization potential, embryo development and pregnancy outcomes in globozoospermic candidates for ICSI and artificial oocyte activation. Andrology Sep; 4(5): Kargar- Dastjerdy P, Tavalaee M, Salehi M, Falahati M, Izadi T, Nasr- Esfahani MH. Altered expression of KLC3 may affect semen parameters. Int J Reprod Biomed (Yazd) Jan; 14(1):15-22.

7 7 8. Kamali-Dolat Abadi M, Tavalaee M, Shahverdi AH, Nasr-Esfahani MH. Evaluation of PLCζ and PAWP expression in globozoospermic individuals. Cell J Fall; 18(3): Barekat F, Tavalaee M, Deemeh MR, Bahreinian M, Azadi L, Abbasi H, Rozbahani SH, Nasr-Esfahani MH. A Preliminary Study: N-acetyl-L-cysteine Improves Semen Quality Following Varicocelectomy. Int J Fertil Steril Apr-Jun; 10(1): Javadian-Eliaderani S, Ghaedi K, Tavalaee M, Rabiee F, Deemeh MR, Nasr-Esfahani MH. Diagnosis of genetic defects through parallel assessment of PLCζ and CAPZA3 in infertile men with history of failed oocyte activation. Iranian Journal of Basic Medical Sciences. Iran J Basic Med Sci Mar; 19(3): Bahreinian M, Tavalaee M, Abbasi H, Kiani-Esfahani A, Shiravi AH, Nasr-Esfahani MH. DNA hypomethylation predisposes sperm to DNA damage in individuals with varicocele. Syst Biol Reprod Med. 2015; 61(4): Tavalaee M, Bahreinian M, Barekat F, Abbasi H, Nasr-Esfahani MH. Effect of varicocelectomy on sperm functional characteristics and DNA methylation. Andrologia Oct; 47(8): Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Health of Children Born Through Artificial Oocyte Activation: A Pilot Study. Reprod Sci. Reprod Sci Mar; 22(3): Afiyani AA, Deemeh MR, Tavalaee M, Razi M, Bahadorani M, Shokrollahi B, Nasr-Esfahani MH. Evaluation of heat-shock protein A2 (HSPA2) in male rats before and after varicocele induction. Mol Reprod Dev Aug; 81(8): Bateni Z, Azadi L, Tavalaee M, Kiani-Esfahani A, Fazilati M, Nasr-Esfahani MH. Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function. Syst Biol Reprod Med Aug; 60(4): Epub 2014 Apr Tavalaee M, Deemeh MR, Arbabian M, Kiyani A, Nasr-Esfahani MH. Relationship between fertilization rate and early apoptosis in sperm population of infertile individuals. Andrologia. Andrologia Feb; 46(1):36-41.

8 8 17. Hosseinpour E, Shahverdi A, Parivar K, Sedighi Gilani MA, Nasr-Esfahani MH, Salman Yazdi R, Sharbatoghli M, Tavalaee M, Chehrazi M. Sperm ubiquitination and DNA fragmentation in men with occupational exposure and varicocele. Andrologia May; 46(4): Epub 2013 Apr Charehjooy N, Najafi MH, Tavalaee M, Deemeh MR, Azadi L, Shiravi AH, Nasr-Esfahani MH. Selection of Sperm Based on Hypo-Osmotic Swelling May Improve ICSI Outcome: A Preliminary Prospective Clinical Trial. Int J Fertil Steril Apr; 8(1):21-8. Epub 2014 Mar Kiani-Esfahani A, Bahrami S, Tavalaee M, Deemeh MR, Mahjour AA, Nasr-Esfahani MH. Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling? Syst Biol Reprod Med Dec; 59(6): Epub 2013 Aug Bassiri F, Tavalaee M, Nasr Esfahani MH. Correlation between Different Patterns of Hypo-Osmotic Swelling and Sperm Functional Tests. Int J Fertil Steril Oct; 7(3): Epub 2013 Sep Zahedi A, Tavalaee M, Deemeh MR, Azadi L, Fazilati M, Nasr-Esfahani MH. Zeta potential vs apoptotic marker: which is more suitable for ICSI sperm selection? J Assist Reprod Genet Sep; 30(9): Epub 2013 Jul Eskandari-Shahraki M, Tavalaee M, Deemeh MR, Jelodar GA, Nasr-Esfahani MH. Proper ubiquitination effect on the fertilization outcome post-icsi. Andrologia Jun; 45(3): Epub 2012 Jul Forouzanfar M, Fekri Ershad S, Hosseini SM, Hajian M, Ostad-Hosseini S, Abid A, Tavalaee M, Shahverdi A, Vosough Dizaji A, Nasr Esfahani MH. Can permeable super oxide dismutase mimetic agents improve the quality of frozen-thawed ram semen? Cryobiology Apr; 66(2): Epub 2012 Dec Motiei M, Tavalaee M, Rabiei F, Hajihosseini R, Nasr-Esfahani MH. Evaluation of HSPA2 in fertile and infertile individuals. Andrologia Feb; 45(1): Epub 2012 Jun Nasr Esfahani, Tavalaee M. Origin and Role of DNA Damage in Varicocele. Int J Fertil Steril Oct; 6(3): Epub 2012 Dec 17.

9 9 26. Tavalaee M, Abbasi H, Deemeh MR, Fotohi F, Sadoughi Gilani MA, Nasr Esfahani MH. Semen parameters and chromatin packaging in microsurgical varicocelectomy patients. Int J Fertil Steril Oct; 6(3): Epub 2012 Dec Nasr-Esfahani MH, Deemeh MR, Tavalaee M. New era in sperm selection for ICSI. Int J Androl Aug; 35(4): Epub 2011 Nov 15. Review. 28. Bassiri F, Tavalaee M, Shiravi AH, Mansouri S, Nasr-Esfahani MH. Is there an association between HOST grades and sperm quality? Hum Reprod Aug; 27(8): Epub 2012 May Kiani-Esfahani A, Tavalaee M, Deemeh MR, Hamiditabar M, Nasr-Esfahani MH. DHR123: an alternative probe for assessment of ROS in human spermatozoa. Syst Biol Reprod Med Jun; 58(3): Epub 2012 Apr Navaeian-Kalat E, Deemeh MR, Tavalaee M, Abasi H, Modaresi M, Nasr-Esfahani MH. High total acrosin activity in varicocele individuals. Andrologia May; 44 Suppl 1: Epub 2011 Nov Zarei-Kheirabadi M, Shayegan Nia E, Tavalaee M, Deemeh MR, Arabi M, Forouzanfar M, Javadi GR, Nasr-Esfahani MH. Evaluation of ubiquitin and annexin V in sperm population selected based on density gradient centrifugation and zeta potential (DGC-Zeta). J Assist Reprod Genet Apr; 29(4): Epub 2011 Dec Tavalaee M, Deemeh MR, Arbabian M, Nasr-Esfahani MH. Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection? J Assist Reprod Genet Jan; 29(1):31-8. Epub 2011 Dec Aghajanpour S, Ghaedi K, Salamian A, Deemeh MR, Tavalaee M, Moshtaghian J, Parrington J, Nasr-Esfahani MH. Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample. Hum Reprod Nov; 26(11): Epub 2011 Sep Fathi Z, Tavalaee M, Kiani A, Deemeh MR, Modaresi M, Nasr-Esfahani MH. Flow Cytometry: A Novel Approach for Indirect Assessment of Protamine Deficiency by CMA3 Staining, Taking into Account the Presence of M540 or Apoptotic Bodies. Int J Fertil Steril Oct; 5(3): Epub 2011 Dec 22.

10 Azadi L, Abbasi H, Deemeh MR, Tavalaee M, Arbabian M, Pilevarian AA, Nasr-Esfahani MH. Zaditen (Ketotifen), as mast cell blocker, improves sperm quality, chromatin integrity and pregnancy rate after varicocelectomy. Int J Androl Oct; 34(5 Pt 1): Epub 2010 Sep Nasr-Esfahani MH, Tavalaee M, Arbabian M, Parrington J. Can Assessment of Total Acrosin Activity Help Predict Failed or Low Fertilization Rate ICSI for Implementation of Artificial Oocyte Activation? The Open Andrology Journal, 2010; 2: Soleimani M, Tavalaee M, Aboutorabi R, Adib M, Bahramian H, Janzamin E, Kiani A, Nasr-Esfahani MH. Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals. J Assist Reprod Genet Aug; 27(8): Epub 2010 Jun Nasr-Esfahani MH, Deemeh MR, Tavalaee M. Artificial oocyte activation and intracytoplasmic sperm injection. Fertil Steril Jul; 94(2): Epub 2009 Apr 25. Review. 39. Deemeh MR, Tavalaee M, Ahamadi SM, Kalantari SAA, Alavi Nasab SV, Najafi MH, Nasr-Esfahani MH. The First Report of Successfully Pregnancy After ICSI with Combined DGC/Zeta Sperm Selection Procedure in a Couple with Eleven Repeated Fail IVF/ICSI Cycles. Int J Fertil Steril Apr-Jun; 1(4): Tavalaee M, Kiani A, Arbabian M, Deemeh MR, Nasr-Esfahani MH. Flow Cytometry: A New Approach for Indirect Assessment of Sperm Protamine Deficiency. Int J Fertil Steril Feb-Mar; 4(3): Hatami-Baroogh L, Razavi S, Zarkesh-Esfahani H, Tavalaee M, Tanhaei S, Ghaedi K, Deemeh MR, Rabiee F, Nasr-Esfahani MH. Evaluation of the leptin receptor in human spermatozoa. Reprod Biol Endocrinol Feb;( 8): Razavi SH, Nasr-Esfahani MH, Deemeh MR, Shayesteh M, Tavalaee M. Evaluation of zeta and HA-binding methods for selection of spermatozoa with normal morphology, protamine content and DNA integrity. Andrologia Feb; 42(1): Nasr Esfahani MH, Abbasi H, Mirhosseini Z, Ghasemi N, Razavi S, Tavalaee M, Tanhaei S, Deemeh MR, Ghaedi K, Zamansoltani F, Rajaei F. Can Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis? Int J Fertil Steril Oct-Dec; 3(4):

11 Babazadeh Z, Razavi S, Tavalaee M, Deemeh MR, Shahidi M, Nasr-Esfahani MH. Sperm DNA damage and its relation with leukocyte DNA damage. Reprod Toxicol Jan; 29(1): Epub 2009 Sep Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh MR, Mardani M, Moshtaghian J, Nasr-Esfahani MH. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod Oct; 24(10): Epub 2009 Jun Tavalaee M, Razavi S, Nasr-Esfahani MH. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril Apr; 91(4): Epub 2008 May Nasr-Esfahani MH, Abasi H, Razavi S, Ashrafi S, Tavalaee M. Varicocelectomy: semen parameters and protamine deficiency. Int J Androl Apr; 32(2): Epub 2007 Oct Khajavi NA, Razavi S, Mardani M, Tavalaee M, Deemeh MR, Nasr-Esfahani MH. Can Zeta sperm selection method, recover sperm with higher DNA integrity compare to density gradient centrifugation? Iranian Journal of Reproductive Medicine. 2009; 7(2): Nasr-Esfahani MH, Razavi S, Javdan Z, Tavalaee M. Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection. Fertil Steril Dec; 90(6): Epub 2008 Jan Nasr-Esfahani MH, Razavi S, Vahdati AA, Fathi F, Tavalaee M. Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome. J Assist Reprod Genet May; 25(5): Epub 2008 May Salamian A, Ghaedi K, Razavi S, Tavalaee M, Tanhaei S, Salahshouri I, Gourabi H, Nasr-Esfahani MH. Single Nucleotide Polymorphism Analysis of Protamine Genes in Infertile Men. Int J Fertil Steril. May-Jun 2008; 2(1): Nasr-Esfahani MH, Razavi S, Tavalaee M. Failed fertilization after ICSI and spermiogenic defects. Fertil Steril Apr; 89(4): Epub 2007 Jun Tavalaee M, Nasr Esfahani MH, Deemeh MR. Etiology and Evaluation of Sperm Chromatin Anomalies. Int J Fertil Steril May-Jun; 1(2):1-43.

12 Tavalaee M, Razavi S, Nasr-Esfahani MH. Effect of sperm acrosomal integrity and protamine deficiency on IVF and pregnancy rate. Int J Fertil Steril. 2007; 1(1): Deemeh MR, Tavalaee M, Razavi S, Nasr-Esfahani MH. Evaluation of protamine deficiency and DNA fragmentation in two globozoospermia patients undergoing ICSI. Int J Fertil Steril. 2007; 1(2): ) Articles published in National Research & Scientific Journals: Navaeian-kalat E, Tavalaee M, Azadi L, Abbasi H, Deemeh MR, Arbabian M, Nasr-Esfahani MH. Known pathophysiological mechanisms in the incidence of varicoceles. J Shahrekord Univ Med Sci. 2016; 18(1): Barekat F, Tavalaee M, Azadi L, Nasr-Esfahani MH. Comparison of sperm parameters and its functional characteristics between fertile and infertile men with varicocele. Cell and Tissue. 2016; 6(4): Tavalaee M, Parivar K, Javadian-Eliaderani S, Nasr-Esfahani MH. Centrosome and its role in gametogenesis and embryonic development. J Shahrekord Univ Med Sci. 2016; 18(2): Tavalaee M, Parivar K, Nasr-Esfahani MH, Shahverdi AH, Ghaedi k. Comparison of chromatin structure and PLCζ in sperm from infertile oligoasthenoteratozoospermic men and fertile individuals. J Shahrekord Univ Med Sci In press. 5. Aghajanpour S, Tavalaee M, Azadi L, Nasr-Esfahani MH. The role of phospholipase Czeta in male fertility as an oocyte activation factor. J Shahrekord Univ Med Sci Feb, Mar; 16(6): Shaygan nia E, Tavalaei M, Nasr- Esfahani MH. Role of ubiquitin-proteasome in spermatogenesis process. JIMS. 2014; 288(32):1-16.

13 13 7. Azadi L, Tavalaee M, Nasr-Esfahani MH. Introduction of routine and novel sperm selection methods for treatment infertile patient undergoing ICSI procedure. J Kermanshah Univ Med Sci. 2014; 18(3): Deemeh MR, Tavalaee M, Nasr-Esfahani MH. Artificial Oocyte Activation: Methods and Clinical Efficiency after Intra-Cytoplasmic Sperm Injection. JIMS, 2013 Aug; 240 (31): Motiei M, Tavalaee M, Nasr-Esfahani MH. The role and effect of HSPA2 in male infertility. Journal of Iranian Anatomical Sciences. 2012; 37(9): Review. 10. Navaeian-Kalat E, Tavalaee M, Abasi H, Nasr-Esfahani MH. Comparison of Sperm Parameters and DNA Integrity between Fertile and Varicocele Individuals. Journal of Cell & Tissue (JCT). 2012; 3(2): Nasr-Esfahani MH, Razavi S, Mardani M, Khajavi N, Deemeh MR, Tavalaee M. The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure. Cell Journal. 2009; 11(2): Deemeh MR, Nasr-Esfahani MH, Razavi S, Nazem HA, Moghadam S, Tavalaee M. The comparison of HA binding and Zeta methods efficiency in selection of sperm with normal morphology and intact chromatin. JIMS. 2009; 27(92): Taghadosi MT, Tavalaee M, Razavi S, Mardani M, Nasr Esfahani MH. Efficiency of Sperm Gradient Processing and HA-binding Test for the Selection of Sperm with Normal Protamine, intact DNA and Normal Morphology. Cell Journal. 2008; 9(4): Razavi S, Nasr-Esfahani MH, Tavalaee M, Ameri A. Assessing the sperm DNA damages on Assisted Reproductive Technology outcome. Arak Medical University Journal. 2008; 11(1):21-31.

14 Nasr-Esfahani MH, Razavi S, Vahdati AA, Fathi F, Tavalaee M. Evaluation of sperm selection procedure based on its binding ability to hyaluronic acide on ICSI outcome. JIMS. 2008; 26(89): Nasr-Esfahani MH, Tavalaee M, Deemeh MR. Origins and Evaluation of DNA Damage in Infertile Individual. Journal of Iranian Anatomical Sciences. 2008; 6(24): Fathi F, Vahdati A, Nasr-Esfahani MH, Razavi S, Tavalaee M. Relation between sperm binding ability to hyaluronic acid with human sperm parameters. Iranian biology Journal. 2008; 21(5): Moghadam S, Nasr-Esfahani MH, Razavi S, Nazem HA, Deemeh MR, Tavalaee M.The efficiency of zeta method in separation of sperm with normal morphology and chromatin structure. JSKUMS, 2008; 10(1): Ashrafi S, Nasr-Esfahani MH, Razavi S, Abasi H, Molavi F, Tavalaee M. Effect of varicocelectomy on semen parameters and protamine deficiency 3 and 6 months post-surgery. JIMS, 2007; 87(25): Razavi S, Fathi F, Tavalaee M, Vahdati A, Nasr Esfahani MH. Relation between sperm binding ability to hyaluronic acid with protamine deficiency and DNA fragmentation. Journal of Iranian Anatomical Sciences. 2007; 5(18): Tavalaee M, Razavi S, Nasr-Esfahani MH. Effects of Spermiogenic Defects on Fertilization and Pregnancy Rate in IVF Patients. Cell Journal. 2007; 9(2): Razavi S, Nasr-Esfahani MH, Tavalaee M, Vaezi GH, Shiravi A. Relationship between protamine deficiency and acrosin activity with fertilization rate post ICSI. Journal of Iranian Anatomical Sciences, 2006; 3(4): Nasr Esfahani MH, Razavi S, Vaezi GH, Shiravi E, Tavalaee M. Effects of acrosomal activity and morphology on fertilization rates following ICSI. Journal of reproduction and infertility. 2006; 7(3):

15 15 6) Conferences and Meetings:65 NO TITLE AUTHOR LOCATION PRES. DATE 1 The role of sperm markers involved in oocyte activation in infertile men with previous failed fertilization Tavalaee M 1 st National Symposium of sperm biology speaker Novel sperm selection procedures in ICSI Tavalaee M 2 st workshop novel and routine normal sperm selection for infertile men procedures speaker Comparison of two novel sperm selection procedures: Zeta potential vs MACS Tavalaee M, Nasr Esfahani The 16th Royan International Congress on Reproductive Biomedicine Poster Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Norozi, Tavalaee M, Bahadorani, Nasr Esfahani The 16th Royan International Congress Poster 2015

16 Men with Severe Semen Parameters Efficiency of Magnetic-Activated Cell Sorting System in Separation of Normal Sperm Effect of Calcium Ionophore A23187 on Sperm Hyper-Active Motility Patterns Evaluation of quantitative expression 6 of PLCζ, PAWP and tr-kit as prediction factors in successful fertilization after ICSI combined AOA Efficiency of Magnetic-Activated 7 Cell Sorting for Sperm Preparation before and after Density Gradient Centrifugation. Study of Hyper-Activation Pattern of 8 Motility and Acrosome Reaction after Density Gradient Centrifugation Cellular, Molecular and Clinical 9 perspective of Varicocele 10 Evaluation sperm DNA damage and its influence on infertility Ziarati, Tavalaee M, Bahadorani, Nasr Esfahani Torki, Bahadorani, Tavalaee M, Nasr Esfahani Tavalaee M, Nasr-Esfahani Tavalaee M, Deemeh, Arbabian, Nasr-Esfahani Torki, Bahadorani Tavalaee M, Nasr-Esfahani Navaeian-kalat, Tavalaee M, Abasi, Nasr-esfahani Tavalaee M on Reproductive Biomedicine The 16th Royan International Congress on Reproductive Biomedicine The 16th Royan International Congress on Reproductive Biomedicine International congress on reproduction (ISERB) International congress on reproduction (ISERB) International congress on reproduction (ISERB) International congress on reproduction (ISERB) 1 st National Symposium of the Embryology Updates on the Research and Poster 2015 Poster 2015 Panel 2015 speaker 2015 speaker 2015 Poster 2015 Oral 2014

17 17 Therapy (Workshop) 11 Assessment of sperm chromatin and morphology in different patterns of HOST and clinical outcome after ICSI Evaluation of human sperm acrosome reaction in the PVP condition Assessment of HSPA2 before and after varicocele induction in rat sperm Effect of Tempol and quercitin on human sperm and viability during cryopreservation Basiri F, Tavalaee M, Nasr- Esfahani MH Torki B, Tavalaee M, Azadi L, Bahadorani M, Nasr- Esfahani MH Atrian A, Deemeh MR, Tavalaee M, Nasr-Esfahani MH Azadi L, Tavalaee M, Deemeh MR, Arbabian M, Nasr-Esfahani MH 1 st National Symposium of the Embryology Updates on the Research and Therapy 1 st National Symposium of the Embryology Updates on the Research and Therapy 1 st National Symposium of the Embryology Updates on the Research and Therapy 1 st National Symposium of the Embryology Updates on the Research and Therapy Oral 2014 Poster 2014 Poster 2014 Poster Comparison of sperm quality, oxidative stress, DNA fragmentation, protamine deficiency, and DNA methylation in varicocele and fertile Bahreinian M, Tavalaee M, Nasr-Esfahani MH 15 th Congress on Poster 2014

18 18 individuals 16 Effect of Centrifugation on Levels of Stress Oxidative of Human Spermatozoa Azadi L, Tavalaee M, Deemeh MR, Arbabian M, Nasr-Esfahani MH 15 th Congress on Poster Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging, Miscarriage and Pregnancy Rate in Infertile Men With Varicocele Tavalaee M, Deemeh MR, Nasr Esfahani MH 15 th Congress on Poster Evaluation of heat shock protein A2 (HSPA2) in male rats before and after varicocele induction Atrian Afiyani A, Deemeh MR, Tavalaee M, Nasr- Esfahani MH 15 th Congress on Poster Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging, Miscarriage and Pregnancy Rate in Infertile Men With Varicocele Tavalaee M 15 th Congress on Poster Comparison of human sperm capacitation status using the measured hyper-activation motility pattern between the DGC and DGC- PVP procedures Torki Boldaji B, Azadi L, Tavalaee M, Bahadorani M, Nasr-Esfahani MH 20 th National Congress of Reproductive Medicine Poster Comparison of Zaditen and NAC on Deemeh MR, Azadi L, 20 th National Poster 2014

19 19 semen parameters of individuals with varicocele after varicocelectomy Arbabian M, Tavalaee M, Abbasi H, Nasr Esfahani MH Congress of Reproductive Medicine 22 Evaluation of sperm chromatin structure and morphology in different patterns of HOST positive sperm Bassiri F, Tavalaee M, Nasr- Esfahani MH 20 th National Congress of Reproductive Medicine Poster Evaluation of Tempol effects on semen quality during human sperm cryopreservation Azadi L, Deemeh MR, Arbabian M, Tavalaee M 20 th National Congress of Reproductive Medicine Poster Assessment of sperm parameters and chromatin status in rat experimental varicocele Atrian Afiyani A, Azadi L, Deemeh MR, Tavalaee M, Nasr-Esfahani MH 20 th National Congress of Reproductive Medicine Poster KLC3-gene expression comparison between fertile and infertile individuals 26 Association between fertilization rate and externilization of phosphatidyl serine Kargar Dastjerdy P, Azadi L, Deemeh MR, Tavalaee M, Salehi M, Falahaty M, Nasr- Esfahani MH Tavalaee M, Deemeh MR, Kiyani MH, Nasr-Esfahani MH 20 th National Congress of Reproductive Medicine 14 th Congress on Poster 2014 Poster 2013

20 21 27 Which novel sperm selection is suitable for ICSI? MACS-DGC or DGC- MACS 28 Comparison of sperm Acrosin Activity and DNA fragmentation between varicocele and infertile individuals 29 Varicocele improved semen quality and chromatin integrity in normozoospermic and nonnormozoospermic varicocele individual Tavalaee M, Deemeh MR, Nasr-Esfahani MH Navaeian Kalat E, Tavalaee M, Abbasi H, Nasr-Esfahani MH Deemeh MR, Tavalaee M, Nasr- Esfahani MH 14th Congress on Poster th Congress on Poster th Congress on Poster HOST patterns and sperm quality Bassiri F, Tavalaee M, Nasr- Esfahani MH 14th Congress on Poster Sperm selection based on surface charge: improvement of embryo quality in teratozoospermic individuals 32 Effect of NAC after varicocelectomy on sperm quality of varicocele individuals Deemeh MR, Tavalaee M, Azadi L, Nasr-Esfahani MH Barekat F, Tavalaee M, Rozbahani SH, Nasr- Esfahani MH, 14th Congress on 14th Congress on Poster 2013 Poster Comparison of semen parameters, Barekat F, Deemeh MR, 14th Congress on

21 21 sperm DNA integrity and oxidative stress between fertile and varicocele Tavalaee M, Nasr-Esfahani MH Poster Comparison of sperm quality, oxidative stress, DNA fragmentation, protamine deficiency, and DNA methylation in varicocele and fertile individuals Bahrainian M, Tavalaee M, Kiani A, Shiravi AH, Nasr- Esfahani MH 14th Congress on Poster Pregnancy rate after varicocelectomy: Long-term outcome in a large number of varicocele individuals Azadi L, Tavalaee M, Deemeh MR, Abbasi H, Nasr-Esfahani MH 14th Congress on Poster Evaluation of heat shock protein A2 (HSPA2) in male rats before and after varicocele induction Atrian Afiyani A, Deemeh MR, Bahadorani M, Tavalaee M, Shokrollahi B, Nasr-Esfahani MH 14th Congress on Poster Assessment of sperm chromatin status in rat experimental varicocele Atrian Afiyani A, Deemeh MR, Bahadorani M, Mazdak M, Tavalaee M, Nasr- Esfahani MH 14th Congress on Poster 2013

22 22 38 Quantitative assessment of human sperm transcripts as genetic fingerprints of male infertility Nasr-Esfahani MH, Tavalaee M, Tanhaee S, Ghaedi K, Deemeh MR, Javadian S, Aghajanpour S, Salamian A 4th congress of the Asia Pacific Initiative on Reproduction (ASPIRE) Oral Evaluation of HSPA2 in fertile and infertile individuals Motiei M, Tavalaee M, Rabiei F, Hajihosseini R, Nasr-Esfahani MH 13 th Congress on Poster Which Novel Sperm method is Functional? MACS-DGC or DGC-Zeta Zahedi yeganeh A, Tavalaee M, Fazilati,. Nasr-Esfahani MH 13 th Congress on Poster Direct Evaluation of HspA2 in fertile and infertile individuals Motiei M, Tavalaee M, Nasr-Esfahani MH 12th Congress on Poster Phospholipase C Zeta: as an Index to Assess Fertilization Potential of a Semen Sample Aghajanpour S, Ghaedi K, Salamian A, Tavalaee M, Deemeh MR, Nasr-Esfahani MH 12th Congress on Poster 2011

23 23 43 Zaditen (Ketotifen), as mast cell blocker, improves sperm quality, chromatin integrity and pregnancy rate after varicocelectomy Azadi L, Tavalaee M, Deemeh MR, Nasr-Esfahani MH 12th Congress on Poster Evaluation of sperm acrosin activity and chromatin structure in varicocele patients before and after varicocelectomy Navaeian Kalat E, Tavalaee M, Deemeh MR, Nasr- Esfahani MH 12th Congress on Poster Assessment of Apoptosis Marker in Novel Sperm Selection Procedure Zarei M, Tavalaee M, Deemeh MR, Nasr-Esfahani MH 12th Congress on Poster Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies Fathi Z, Tavalaee M, Deemeh MR, Nasr-Esfahani MH 12th Congress on Royan International Poster DHR 123: A suitable fluorescent mitochondrial probe for assessment of H2O2 in human spermatozoa Evaluation sperm DNA damage and its influence on infertility Kiani-Esfahani A, Tavalaee M, Deemeh MR, Hamiditabar M, Nasr Esfahani MH Tavalaee M 12th Congress on Royan International 11th Congress on Poster 2011 Oral 2010

24 24 49 Influence of sperm chromatin anomalies on ART outcome Tavalaee M 9 th Congress on Reproductive Biomedicine Oral The comparison of density gradiant centrifugation and Zeta methods in separation of mature and normal spermatozoa by TUNEL, SCD, AO and CMA3 Zeta method: a novel sperm selection method for ICSI with normal chromatin structure Khajavi, Razavi S, Tavalaee M, Deemeh MR, Nasr- Esfahani MH Kheirollahi M, Nasr- Esfahani MH, Razavi S, Moshtaghian J, Tavalaee M, Deemeh MR 9 th Congress on Reproductive Biomedicine 9 th Congress on Reproductive Biomedicine Oral 2008 Poster Relationship between expression levels of HSPA2 gene and chromatin structure in patients with varicocele Mir Hosseini Z, Nasr- Isfahani MH, Razavi S, Rezazade H, Tanhaei S, Deemeh MR, Ghasemi N, Tavalaee M 9 th Congress on Reproductive Biomedicine Poster Existence of leptin s receptor (Ob-R) in spermatozoa from infertile males Hatami.L, Nasr-Esfahani. MH, Razavi S, Zarkesh H, Deemeh MR, Tavalaee M 9 th Congress on Reproductive Biomedicine Poster Comparison between HA binding and Zeta method to select mature sperm for ICSI Deemeh M, Nasr-Esfahani. MH, Razavi S, Nazem H, Shayeste Moghadam M, Tavalaee M 9 th Congress on Reproductive Biomedicine Poster 2008

25 Relationship between protamine deficiency and acrosomal structure with pregnancy rate post IVF Sperm selection with normal morphology and chromatin using HA-binding Effect of Ionomycin as oocyte activation factor in oligoasthenoteratozoospermic patients post ICSI Relationship between protamine deficiency and acrosin activity with fertilization rate post ICSI. Effect of varicocelectomy on protamine deficiency 3 and 6 months post surgery. Relationship between banded sperm to acid hyaluronic with DNA fragmentation and sperm parameters. Comparison of sperm parameters before and after varicocelectomy and its effect on fertility. Tavalaee M, Razavi S, Nasr- Esfahani MH Taghadosi MT, Nasr- Esfahani MH, Razavi S, Mardani M, Tavalaee M Razavi S, Javdan Z, Nasr- Esfahani MH, Tavalaee M Tavalaee M, Razavi S, Nasr- Esfahani MH Ashrafi, Nasr-Esfahani MH, Razavi S, Tavalaee M Fathi Z, Nasr-Esfahani MH, Razavi S, Vahdati A, Tavalaee M Ashrafi, Nasr-Esfahani MH, Razavi S, Tavalaee M 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine 13 th Congress Of Iranian society for reproductive medicine Poster 2007 Oral 2007 Oral 2007 Oral 2007 Oral 2007 Oral 2007 Oral 2007

26 26 63 Effects of acrosomal activity and morphology on fertilization rates following ICSI Nasr Esfahani MH, Rezaei S, Tavalaee M.Vaezi G, shiravi E 2 th iranbioconference Poster Relationship between protamine deficiency and acrosin activity with fertilization rate post ICSI Razavi S, Nasr-Esfahani MH, Tavalaee M,Vaezi G, Shiravi E 2 th iranbioconference Poster SNP analyses of protamine genes in infertile men population. Salamyian A, Nasr-Isfahani MH, Ghaedi K, Razavi S, Tanhaei S, Tavalaee M 10th Iranian Genetics Congress, Tehran, Iran Poster ) Projects: 47 No TITLE Director/ DATE Grant Evaluation of PLCζ & PAWP genes expression levels in infertile individuals sperm with globozoospermia Comparison of clinical outcomes in two sperm selection procedures (DGC and MACS- DGC) in infertile men ICSI candidate Comparison of Clinical Outcome of Ionomycin and Strontium Chloride Treated Oocytes in Infertile ICSI Candidate Individuals. Director 2015 Director 2015 Director 2015 Comparison of different sperm selection procedures to select normal Director 2015

27 27 sperm for infertile individuals candidate for ICSI 1 Evaluation of DNA damage in different types of human abnormal sperm head morphology Director Comparison effect of Tempol and Quercetin on efficiency of human sperm freeze solution Director Comparison of clinical outcomes in two sperm selection procedures (DGC and MACS-DGC) in infertile men ICSI candidate Comparison of clinical outcomes of activated oocyte with ionomycin and strontium chloride in infertile men candidate ICSI (In process) Study of relationship between the KLC3 gene transcription rate with different sperm parameters and comparision of its transcription rate between fertile and infertile Individuals (Asthenozoospermia, Oligozoospermia and Teratozoospermia) (In process) 6 Evaluation of PLCζ & PAWP genes expression levels in infertile individuals sperm with globozoospermia (In process) Comparison of human sperm Capacitation and Acrosome reaction status after density gradient centrifugation (DGC) and DGC-PVP procedures Director Evaluation and comparison of PAWP and PLC zeta gene expression in infertile with high fertilization and infertile with failed fertilization individuals ICSI candidate Assessment of the ratio of sperm sex chromosome X to Y in samples of semen,

28 28 DGC (Density Gradient Centrifugation) and Zeta sperm selection methods by means of Real-Time PCR The effect of N-Acetylcysteine on sperm parameters, sperm chromatin and oxidative stress in infertile men after varicocelectomy:a double-blined controlled randomized Assessment of Antioxidant Effect of MnTBAP in the freezing media on spermatic parameters, membrane integrity and sperm capacitation of ran spermatozoa after freezing thawing Monitoring of babies born after ICSI and artificial oocyte activation with Ionomycin Evaluation of PLC zeta gene expression and correlation its with acrosin activity enzyme and deficiency protamine in ICSI candidate patients Evaluation and comparison of nuclear maturation apoptosis and sperm morphology in selected sperm from different form of HOS positive in infertile individuals Study of correlation between fertilization rate and early apoptosis in sperm population of infertile individuals candidate for ICSI Director Quantitative evaluation of heat shock protein A2 (HSPA2) and sperm chromatin integrity in male rates before and after varicocele induction Evaluation of Tempol supplementation on efficiency of human sperm freezing medium 2011

29 29 18 Comparison of ICSI outcomes during two sperm selection procedures: routine and HOST Study and comparison of sperm oxidative stress, DNA damage and DNA methylation in varicocele individuals before and after varicocelectomy Comparison of efficiency of two method novel sperm selection: DGC-Zeta and MACS-DGC in infertile individulals Comparison of different sperm selection procedures to select normal sperm for infertile individuals candidate for ICSI Director Study and comparison of heat shock protein A2 ( HspA2) in fertile and infertile males Correlation between percentage of sperm ubiquitinated and protamine deficiency with fertilization rate in ICSI candidate patients Comparison on the efficiency of DGC(Density Gradient Centrifugation) and DGC- Zeta methods for selection of human spermatozoa with low ubiquitination The comparison of efficiency of DGC and DGC-Zeta for selection of sperm with lo apoptosis Evaluation and comparison of CAPZA3 gene expression in fertile and infertile individual s sperm and it s correlation with PLC expression and fertilization rate 27 Evaluation of sperm acrosin activity and chromatin structure in varicocele patien before and after varicocelectomy. 2010

30 Evaluation of relation between acrosin activity with artificial activation of oocyte in ICSI patients Comparison of efficiency of density gradient centrifugation (DGC) with combined DGC/Zeta method on ICSI outcome Flow cytometry: a new approach for indirect assessment of protamine deficiency Evaluation of effect of presence and absence and of basal bodies in the results of sperm protamine seficiency in semen sample of infertile individuals by using flow cytometry technique Evaluation of correlation between the percentage of ROS and CMA3 and apoptosis positive sperm in infertile men by flow cytometry Comparison of sperm morphology, DNA fragmentation, protamine deficiency and HSPA2 expression before, three and six month after varicocelectomy Single nucleotides polymorphism Analysis of Protamine Genes in Infertile Men Population. 35 The study of relationship between epigenetic changes and semen parameters, DNA fragmentation, protamine deficiency in ICSI and IVF patients 36 The comparison of efficiency density gradient centrifugation and Zeta methods in separation of mature sperm with normal Protamine and DNA by flow cytometry Director

31 31 37 Evaluation of leptin receptor expression in fertile and infertile men The study of efficiency of HA test and Zeta method in separation of mature sperm 39 The study of relationship between lymphocyte and sperm DNA fragmentation in infertile patient. 40 Evaluation the effect of zadetin (Ketotifen) as mast cell blocker on sperm parameters and sperm chromatin in infertile men before and after varicocelectomy Study of sperm functional testes Sperm selection based on its binding ability to hyaluronic acid for intra cytoplasmic sperm injection The effect of varicocelectomy on recovery of sperm morphology and protamine deficiency at 3 and 6 months post surgery The Study of relation between protamine deficiency and expression of HSPA2 in varicocele patients, before and after of varicocelectomy Study of relationship between protamin deficiency with acrosin activity,sperm normal morphology and fertilization rate in candidat of ICSI infertile couples 2006

32 32 46 Effect of Ionomycin as oocyte activation factor in oligoasthenoteratozoospermic patients post ICSI Effect of sperm chromatin structure on fertility ) Prize: 5 No TITLE AUTHOR STATUS DATE Evaluation of sperm chromatin structure on fertility Evaluation of sperm chromatin structure on fertility Tavalaee, The Winner of Hamandishi research festival on Nasr-Esfahani medical sciences 2007 Tavalaee, Nasr-Esfahani Javan Kharazmi research festival on medical sciences 2008 As excellent researcher in Isfahan city Tavalaee 5th Paghoheshi va fan avari festival 2009 As excellent researcher in Royan Tavalaee 2012 پاوسدهمیه جشى ار تج As excellent researcher Tavalaee 2014 لیل از پژيهشگران ي فىايران برتر کش ر As excellent researcher in Isfahan city Tavalaee 10th Paghoheshi va fan avari festival 2014 As excellent researcher Tavalaee The first International ISERB award 2015

33 33 10) Presentation of Workshop: 3 nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2014; (Isfahan campus). 2 nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2007; (Isfahan campus). 3 nd Royan Workshop on: Evaluation of sperm functional tests in Identification and treatment of infertile individuals. 2009; (Isfahan campus). 1 nd 3nd Royan Workshop on: spermogram and its interpretation in ART (Isfahan campus). First Royan workshop on: novel and routine sperm selection procedures for infertile men (Isfahan campus). 2nd Royan Workshop on: novel and routine sperm selection procedures for infertile men (Isfahan campus). 11) Consulting of thesis: Moloud Rashidi. Evaluation and comparison of three DNA methyltranferases gene expression between individuals with varicocele and fertile men Elahe.Raji. Comparative expression level mir-373 in the sperm of infertile Individuals patients referred to Center Fertility Infertility Esfahan. Islamic Azad University, Shahrekord Branch

34 34 3. Marziyeh Norozi Hafshejani. Comparison of Clinical Outcome Treated Oocyte whit Ionomycin and Strontium Chloride in Infertile ICSI Candidate Individuals. Islamic Azad University Falavarjan Branch Niloofar ziarati. Comparison of Clinical Outcomes in two Sperm Selection Procedures, Routine (DGC) and Novel (MACS-DGC), in Infertile Men ICSI Candidate. Islamic Azad University Falavarjan Branch Bahareh Torki. Study and comparison of human sperm motility patterns and acrosome reaction in the presence of PVP and density gradient centrifugation method (DGC). Falavarjan Branch, Islamic azad University Zahra Bateni. Evaluation of Tempol supplementation on efficiency of human sperm freezing medium. Payame Noor University (Esfahan) Aliyeh Zahedi Yeganeh. Comparison of efficiency of two method noval sperm selection (DGC-ZETA) and (MACS-DGC) in infertile individuals. Payame Noor University (Esfahan) Forogh Barekat. Evaluation of the effect N-Acetylcysteine (NAC) on sperm parameters, sperm chromatin and oxidative stress in infertile men after varicocelectomy. Falavarjan Branch, Islamic azad University Mahsa bahreinian. Evaluation and comparison of stress oxidative, DNA Fragmentation, DNA methylation sperm in varicocele individual before and after of varicocelectomy. Damghan branch, Islamic azad University Nasim Charehjooy. Comparison of ICSI outcomes during two sperm selection procedures: routine and HOST. Damghan branch, Islamic azad University Saman Fekri-Ershad. Assessing antioxidant effects of MnTBAP in the freezing media on spermatic parameters, membrane integrity and sperm capacitation of ram spermatozoa after freezing-thawing. Fars Science and Research Branch, Islamic azad University Marjan Motiei. Study and comparison of heat shock protein A2 ( HspA2) in fertile and infertile males Study and comparison of heat shock protein A2 ( HspA2) in fertile and infertile males. Payame Noor University (Tehran). 13. Farzaneh Bassiri. Evaluation and comparison of sperm morphology,chromatin structure in HOS positive and negative sperm from infertile individuals. Damghan branch, Islamic azad University 2012.

35 Samaneh Aghajanpour. Evaluation of PLC zeta gene expression and correlation its with acrosin activity enzyme and deficiency protamine in ICSI candidate patients. Isfahan University Zohre Fathi. The role of basal bodies on evaluation of protamine deficiency with flowcytometry. Payame Noor University (Esfahan) Masumeh Zareii Kheirabadi. The comparison of efficiency of DGC and DGC-Zeta for selection of sperm with low apoptosis. Shahrekord University Elahe Navaian Kalat. Evaluation of sperm acrosin activity and chromatin structure in varicocele patients before and after varicocelectomy Payame Noor University (Esfahan) Nazem Ghasemi. Comparison of sperm morphology, DNA fragmentation, protamine deficiency and HSPA2 expression before, three and six month after varicocelectomy. Qazvin University of Medical Sciences Mahsa Kheirollahi. Sperm selection based on its binding ability to hyaluronic acid for intra cytoplasmic sperm injection. Isfahan University ) Research Profile (preview): A) Semen Analysis B) Sperm functional testes: B-1) Nuclear chromatin maturity tests: - Chromomycin A3 staining (CMA3)

36 36 - Aniline blue staining (Ab) - Aniline blue-eosin staining (Ab-E) - Acridine orange staining (AO) - Sperm chromatin Dispersion (SCD) - Sperm chromatin Decondensation - Single cell gel electrophoresis (Comet assay) - DNA terminal deoxynucleotidyl transferase-mediated dudp nick-end labelling (TUNEL assay) B-2) Sperm functional tests: - Hypo-Osmotic Swelling Test (HOST) - Gelatinolysis Test as indicator of acrosin activity - Evaluation of acrosin activity by spectrophotometery - CTC test for evaluation of capacitation - Evaluation of sperm ubiquitination, methylation DNA and DNAMTs - Evaluation of sperm apoptotic marker (Annexin and caspase)

37 37 - Evaluation of sperm ROS by DCF staining - PHD test for evaluation of acrosome reaction C) Sperm selection procedures C-1) Routine sperm selection procedures - Swim-up - Density gradient centrifugation (DGC) C-2) Novel sperm selection procedures - Hyaluronic acid (HA) binding method based on HA receptor - Zeta method based on sperm surface charges - Combined density gradient and Zeta method - MACS method based on apoptotic marker -HOS method based on sperm membrane integrity

38 38 D) Human sperm freezing E) Techniques - Real time PCR - Western blot 13) Patent - Immunofluorescence

39 39