Chapter 5. Transportation of Oocyte & Embryo

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1 46 Chapter 5 Transportation of Oocyte & Embryo

2 Transportation of Oocyte & Embryo Chapter 5 47

3 Chapter 5 Transportation of Oocyte & Embryo 48

4 Transportation of Oocyte & Embryo Chapter 5 49

5 Chapter 5 Transportation of Oocyte & Embryo 50

6 Transportation of Oocyte & Embryo Chapter 5 51

7 52 Chapter 5 Transportation of Oocyte & Embryo

8 Transportation of Oocyte & Embryo Chapter 5 Note 53

9 Chapter 6 Cryopreservation of Oocyte & Embryo 54

10 Cryopreservation of Oocyte & Embryo Chapter 6 55

11 Chapter 6 Cryopreservation of Oocyte & Embryo 56

12 Cryopreservation of Oocyte & Embryo Chapter 6 57

13 Chapter 6 Cryopreservation of Oocyte & Embryo 58

14 Cryopreservation of Oocyte & Embryo Chapter 6 59

15 Chapter 6 Cryopreservation of Oocyte & Embryo 60

16 Cryopreservation of Oocyte & Embryo Chapter 6 61

17 62 Chapter 6 Cryopreservation of Oocyte & Embryo

18 Cryopreservation of Oocyte & Embryo Chapter 6 63

19 Chapter 7 Other Techniques 64

20 65 Chapter 7 Other Techniques

21 Chapter 7 Other Techniques 66

22 Other Techniques Chapter 7 67

23 68 Chapter 7 Other Techniques Note

24 Other Techniques Chapter 7 69

25 Chapter 7 Other Techniques 70

26 Other Techniques Chapter 7 71

27 72 Chapter 7 Other Techniques

28 Other Techniques Chapter 7 73

29 Chapter 7 Other Techniques 74

30 Other Techniques Chapter 7 75

31 Chapter 7 Other Techniques 76

32 Other Techniques Chapter 7 77

33 Chapter 8 Media 78

34 Media Chapter 8 79

35 Chapter 8 Media 80

36 Media Chapter 8 81

37 Chapter 8 Media 82

38 Media Chapter 8 83

39 Chapter 8 Media 84

40 Media Chapter 8 85

41 Chapter 8 Media 86

42 Media Chapter 8 87

43 Chapter 8 Media 88

44 Media Chapter 8 89

45 Information 90

46 FERTIUP Mouse in vitro Fertilization Medium Cryoprotectant Preincubation Medium CARD MEDIUM IVF Slump? FERTIUP Will Get You Out!! FERTIUP and CARD MEDIUM are valuable reagents to improve the recovery of frozen mouse spermatazoa and improve in vitro fertilization efficiency of laboratory mice. Combined usage of FERTIUP Cryoprotectant, FERTIUP Preincubation Medium and CARD MEDIUM Mouse Fertilization Medium offers the following benefits: Fertilization rates over 80% Improved management of transgenic mouse Reduction of labor, facilities and breeding costs Reduction of colony expansion time Efficient production in difficult breeding in vitro fertilization rate (%) Fertilization of cryopreserved and thawed sperm using FERTIUP and CARD MEDIUM Conventional usual method method Fertilization rate rate 30% < < FERTIUP Fertilization rate rate 80% < FERTIUP + CARD MEDIUM Description Cat. No. Quantity FERTIUP Cryoprotectant: CPA KYD-001-EX 1 ml KYD-001-EX-X5 KYD EX CARD MEDIUM KYD-003-EX 1 kit FERTIUP PM 1ML-CARD MEDIUM set KYD-004-EX 1 set FERTIUP Preincubation Medium: PM (1 ml) x 1 vial, CARD MEDIUM x 1 kit FERTIUP PM 0.5ML-CARD MEDIUM set KYD-005-EX 1 set FERTIUP Preincubation Medium: PM (0.5 ml) x 1 vial, CARD MEDIUM x 1 kit 5 x 1 ml 0.5 ml KYD EX-X5 5 x 0.5 ml FERTIUP Preincubation Medium: PM KYD-002-EX 1 ml KYD-002-EX-X5 KYD EX KYD EX-X5 5 x 1 ml 0.5 ml 5 x 0.5 ml

47 CARD COLD TRANSPORT KIT Specially designed for inexpensive, safe transport of mouse cauda epididymides and embryos at cold temperature. Reduce cost for transportation of live mice Eliminate risk of mouse fatalities or escape during transportation Prevent transmission of pathogens Valuable for the Rescue in vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa Description Cat. No. Quantity Card Cold Transport Kit KYD-006-EX 1 set Foam transport box (1 box), Cold packs (4 large packs, 2 small packs), Thermos bottle (1 bottle), Paper box (1 box), Shock-absorbing material (1 piece) FERTIUP & CARD MEDIUM peripheral products Description Cat. No. Quantity Sperm Straws (10 Pieces x 10 Units) KYD-S020X10 10 pc Freezing Canister KYD-S018 Freezing Canister KYD-S018 1 unit Straw Connector ( 5 parts included) KYD-S025 1PC Triangular Cassette Short (10units) KYD-S unit Triangular Cassette Long (10units) KYD-S unit Embryo Manipulation Instrument Set KYD-S036 1 set Glass capillary 20PC KYD-S037 1 set Touch Burner APT-3 PHD-APT3-EX 10 unit Straw Connector KYD-S025 Embryo Manipulation Instrument Set KYD-S036 Triangular Cassette Long KYD-S035 Triangular Cassette Short KYD-S021 Sperm Straws KYD-S020X10 Touch Burner APT-3 PHD-APT3-EX Search! fertiup

48 Enhanced Superovulation Reagent for Mouse Live IVF pups from A SINGLE C57BL/6J female following superovulation with CARD HyperOva

49 Superovulation Reagent for mouse 1 ml Store at -80 C to -20 C For research use only. Not for human or medicinal use. Cat. No. KYD-010-EX Manufactured by KYUDO CO., LTD. Search! card hyperova

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FERTIUP PM 1 ml / 0.5 ml - CARD MEDIUM Set

FERTIUP PM 1 ml / 0.5 ml - CARD MEDIUM Set Product manual FERTIUP PM 1 ml / 0.5 ml - CARD MEDIUM Set Cat. No. KYD-004-EX Size: 1 SET KYD-005-EX 1 SET Department of Reproductive Engineering, Center for Animal Resources and Development, Kumamoto

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