Reproductive Engineering Techniques in Mice
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- Duane Cunningham
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1
2 Reproductive Engineering Techniques in Mice
3 Preface In recent years, the number of genetically engineered mice being produced has increased dramatically. Moreover, the rapid progress in the development of genome-editing (TALEN and CRISPR/Cas9) techniques for molecular biology research has been remarkable, so much so that a genetically engineered mouse strain can be produced easily in a few months. Production has been supported by reproductive engineering techniques such as in vitro fertilization, embryo and sperm cryopreservation, and embryo transfer techniques. Such techniques have become invaluable peripheral technologies, and their use is expanding rapidly. This rapid expansion has led to the publication of many technical manuals relating to reproductive engineering techniques in mice hereto. However, mouse reproductive engineering techniques mainly involve delicate operations under a stereoscopic microscope, which means that a sufficiently detailed technical manual has not yet been published. With that in mind, in this book we aim to create a mouse reproductive engineering technique manual that can be easily understood by anyone. We included a generous number of diagrams, photographs and videos in our manual, and explained each step of each reproductive engineering technique as clearly and thoroughly as we could. We sincerely hope that our manual will become the definitive guide for students, technicians, researchers and other people wishing to study mouse reproductive engineering techniques. Naomi Nakagata
4 CONTENTS In Vitro Fertilization (IVF) Transportation of sperm Cryopreservation of sperm Preparation of Oocyte & Embryo Transportation of Oocyte & Embryo Cryopreservation of Oocyte & Embryo Other Techniques Media 3
5 4 Chapter 1 In Vitro Fertilization (IVF)
6 In Vitro Fertilization (IVF) Chapter 1 5
7 Chapter 1 In Vitro Fertilization (IVF) 6
8 In Vitro Fertilization (IVF) Chapter 1 7
9 Chapter 1 In Vitro Fertilization (IVF) 8
10 In Vitro Fertilization (IVF) Chapter 1 9
11 Chapter 1 In Vitro Fertilization (IVF) 10
12 11 Chapter 1 In Vitro Fertilization (IVF)
13 Chapter 1 In Vitro Fertilization (IVF) 12
14 In Vitro Fertilization (IVF) Chapter 1 13
15 14 Chapter 2 Transportation of Sperm
16 Transportation of Sperm Chapter 2 15
17 Chapter 2 Transportation of Sperm 16
18 Transportation of Sperm Chapter 2 17
19 Chapter 2 Transportation of Sperm 18
20 Transportation of Sperm Chapter 2 19
21 20 Chapter 3 Cryopreservation of sperm
22 Cryopreservation of sperm Chapter 3 21
23 Chapter 3 Cryopreservation of sperm 22
24 Cryopreservation of sperm Chapter 3 23
25 Chapter 3 Cryopreservation of sperm 24
26 Cryopreservation of sperm Chapter 3 25
27 Chapter 3 Cryopreservation of sperm 26
28 Cryopreservation of sperm Chapter 3 27
29 28 Chapter 3 Cryopreservation of sperm
30 Cryopreservation of sperm Chapter 3 29
31 Chapter 3 Cryopreservation of sperm 30
32 31 Chapter 3 Cryopreservation of sperm
33 Chapter 3 Cryopreservation of sperm 32
34 Cryopreservation of sperm Chapter 3 33
35 Chapter 3 Cryopreservation of sperm 34
36 Cryopreservation of sperm Chapter 3 35
37 Chapter 4 Preparation of Oocyte & Embryo 36
38 Preparation of Oocyte & Embryo Chapter 4 37
39 Chapter 4 Preparation of Oocyte & Embryo 38
40 Preparation of Oocyte & Embryo Chapter 4 39
41 40 Chapter 4 Preparation of Oocyte & Embryo
42 Preparation of Oocyte & Embryo Chapter 4 41
43 Chapter 4 Preparation of Oocyte & Embryo 42
44 Preparation of Oocyte & Embryo Chapter 4 43
45 Chapter 4 Preparation of Oocyte & Embryo 44
46 Preparation of Oocyte & Embryo Chapter 4 45
Chapter 5. Transportation of Oocyte & Embryo
46 Chapter 5 Transportation of Oocyte & Embryo Transportation of Oocyte & Embryo Chapter 5 47 Chapter 5 Transportation of Oocyte & Embryo 48 Transportation of Oocyte & Embryo Chapter 5 49 Chapter 5 Transportation
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