Identification of chromosomes involved in a Robertsonian translocation in cattle

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1 Identification of chromosomes involved in a Robertsonian translocation in cattle E.P. Cribiu, M. Matejka, R. Darre, V. Durand, H.M. Berland, A. Bouvet To cite this version: E.P. Cribiu, M. Matejka, R. Darre, V. Durand, H.M. Berland, et al.. Identification of chromosomes involved in a Robertsonian translocation in cattle. Genetics Selection Evolution, BioMed Central, 1989, 21 (4), pp <hal > HAL Id: hal Submitted on 1 Jan 1989 HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

2 Note Identification of chromosomes involved in a Robertsonian translocation in cattle E.P. Cribiu A. Bouvet 1 M. Matejka 2 R. Darre 2 V. Durand H.M. Berland 2 1 Institut National de la Recherche Agronomique, laboratoire de cytogénétique, centre de recherches de Jouy-en-Josas, Jouy-en-Josas; 2 Ecole Nationale Vétérinaire de Toulouse, laboratoire de cytogénétique, 23, chemin des Capelles, Toulouse Cédex, France (received 29 June 1989; accepted 30 August 1989) Summary - The chromosomes involved in the robertsonian translocation described after classical Giemsa staining by Darre et al. (1974), were determined using G(GTG), R(RBA) and C(CBG) banding techniques. The chromosomes in question were identified as chromosomes 9 and 23. cattle - chromosome - robertsonian translocation Résumé - Les chromosomes impliqués dans une translocation robertsonienne décrite, tout d abord, en coloration conventionnelle par Darre et al. (1974) ont été déterminés à l aide des techniques de marquage G(GTG), R(RBA) et C(CBG). Les chromosomes concernés sont le 9 et le 23. boeuf - chromosome - translocation robertsonienne INTRODUCTION In the field of animal cytogenetics, cattle are the most extensively studied livestock species with about karyotyped animals. Chromosomal abnormalities reported in cattle usually involve robertsonian translocations, among which the most widespread is the 1/29 translocation observed for the first time by Gustavsson and Rockborn (1964) in the Swedish Red and White cattle and reported later in various breeds worldwide (Popescu, 1977). Robertsonian translocations involving other autosomes have also been reported (Berland et al., 1988). However, in contrast to the 1/29 translocation, the other translocations have been reported only as isolated cases and for those described during the early seventies prior to the development of reliable banding methods, the chromosomes involved have not been identified precisely. For example, in 1974 a robertsonian translocation, the socalled "F translocation", was reported in a Blonde d Aquitaine bull without clear identification of the chromosomes involved. Based on chromosomal measurements to the chromosome only, it was suggested that the 2 chromosomes involved belong groups 7 to 11 and 20 to 25, respectively (Darre et al., 1974). In this report, the

3 chromosomes involved in the translocation were identified using the G, C and R banding methods. MATERIALS AND METHODS Animals A total of 16 cows were inseminated with frozen semen from F..., a Blonde d Aquitaine A.I bull carrying two translocations (Darre et al., 1974). The karyotype of the 13 calves produced was studied using blood and tissue cultures. Methods Venous blood samples were collected aseptically from the jugular or the caudal vein, using sterile vacutainer tubes coated with sodium heparin. Whole blood (10 drops) was added to the culture medium containing 9 ml of Ham s F12 growth medium, 20% of fetal calf serum, 25 pg/ml of concanavalin A and various antibiotics. The cultures were incubated at 37 C for 68 h. Colcemid (0.03 ag/ml: final concentration) was added 90 min prior to harvest. After a 10 min hypotonic treatment with potassium chloride (0.56%) and 3, 1-h fixations in modified Carnoy s solution (3:1 absolute ethanol: glacial acetic acid), resuspended cells were spread on glass slides, flame-dried, and either stored unstained at room temperature, or stained for 10 min with a 4% Giemsa solution. Chromosomal analysis was also performed on primary cell cultures established from small dilacerated testicular fragments digested in 2.5 g/1 trypsin solution. Unstained slides were treated for G banding (GTG) using the method of Seabright (1971) and C banding (CBG) following the method of Sumner (1972). For R banding (RBA), 5-bromodeoxyuridine (BrdU, 20 icg/ml) was added to some cultures 8 h before harvesting. The slides were stained with acridine orange (Dutrillaux et al., 1973) and observed under UV light using a Leitz microscope. The chromosomes were identified, paired and arranged according to the recommendations of the 1976 Reading conference (Ford et al., 1980). RESULTS Out of 16 cows inseminated with F... semen, 13 carried to term, 8 males and 5 females. Among the males: 3 had a normal 60,XY karyotype, 4 were carriers of both the F and the 1/29 translocations, and 1 animal carried only the F fusion. Out of 5 females, 1 carried the F fusion, 2 were 1/29 translocation carriers, and 2 carried both abnormalities. The G(GTG) and R(RBA) banding techniques made it possible to confirm the presence of the 1/29 translocation and to identify the chromosomal pairs involved in the F fusion as pairs 9 and 23, respectively (Figs. 1 and 2). The C banding technique revealed the presence of 2 constitutive heterochromatin blocks in the pericentromeric region of the F chromosome and only 1 block on the long arms near the centromere of the 1/29 translocated chromosome (Fig. 3).

4 DISCUSSION AND CONCLUSIONS The 9/23 translocation is, to our knowledge, the third translocation reported in the Blonde d Aquitaine breed, after the 1/29 translocation (Darre et!ll., 1972; Bruere and Chapman, 1973; Popescu, 1973) and the 21/27 translocation (Berland et al., 1988). The 1/29 translocation is widespread in the Blonde d Aq!itaine breed, since the frequency of the hetero and homozygous carriers ranges from 14 to 24% (Queinnec et al., 1974; Cribiu, 1985; Frebling et al., 1987). In contrast, the 21/27 translocation like the 9/23 has been observed only in 1 bull and its progeny (Darre et al., 1974; Berland et al., 1988). Centric fusions or robertsonian translocations are the result from the fusion of 2 acrocentric chromosomes. At least 2 types of robertsonian translocations have been described, depending on the presence of 1 or 2 blocks of constitutive heterochromatin. In the first case, 1 of the chromosomal breakpoints involves arms of the the short arms of 1 chromosome and the other is on the long second chromosome near the centromeric regions. The fusion gives rise to a monocentric meta or submetacentric chromosome and a minute fragment containing the centromere, which is lost during the subsequent cell divisions. On the other hand, if the breakpoints involve only the short arms of both chromosomes in

5 the centromeric region, fusion leads to the formation of a dicentric meta or submetacentric chromosome and the loss of 2 acentric fragments. The use of the C banding technique made it possible to suggest the mechanism by which these 2 translocations arise: the 1/29 chromosome with only 1 constitutive heterochromatin block on the long arms near the centromere could be monocentric (Popescu, 1973; Gustavsson et al., 1976), whereas the 9/23 chromosome with 2 constitutive heterochromatin blocks in the pericentromeric region could be dicentric. According to most authors, dicentric translocations like those already described in cattle are more recent than those which are monocentric like the 1/29 translocation (Eldridge, 1974; Popescu, 1977; Logue and Harvey, 1978; Di Berardino et al., 1979; Ellsworth et al., 1979; Berland et al. 1988). Chromosomal abnormalities associated with morphological defects are rapidly eliminated through selective breeding and do not constitute an important economic drain for the livestock industry. In contrast, animals carrying structural abnormalities like robertsonian translocations do not alter the phenotype, but their fertility is impaired since meiotic segregation leads to unbalanced gametes. Those rearrangements are not easily eliminated by selective breeding and can be spread rapidly through the use of artificial insemination. For example, in cattle, the 1/29 translo-

6 . cation which does not confer any advantage to heterozygous carriers, produces in certain breeds a reduced fertility in the daughters of carrier bulls (Gustavsson, 1969; Refsdal, 1976). ACKNOWLEDGMENTS The help of Mr G. Fabre and his technical staff in the housing and handling of the animals at the Domaine Experimental de Carmaux (INRA) is gratefully acknowledged. REFERENCES Berland H.M., Sharma A., Cribiu E.P., Darre R., Boscher J. & Popescu C.P. (1988) A new case of robertsonian translocation in cattle. J. Hered. 79, Bruere A.N. & Chapman H.M. (1973) Autosomal translocations in two exotic breeds of cattle in New Zealand. Vet. Rec. 92, Cribiu E.P. (1985) Frequence de la translocation 1/29 artificielle franqais. Elev. Ins6min. 209, Darre R., Berland H.M. & Queinnec G. (1974) Une nouvelle translocation robertsonienne chez les bovins. Ann. Genet. Sel. Anim. 6, dans les centres d insemination Darre R., Queinnec G. & Berland H.M. (1972) La translocation 1/29 des bovins. Etude g6n6rale et importance du phénomène dans le Sud-Ouest. Rev. M6d. V6t. 123,

7 Di Berardino D., Iannuzzi L., Farrara L. & robertsonian translocation in cattle. J. Hered. 70, Matassino D. (1979) A new case of Dutrillaux B., Laurent C., Couturier J. & Lejeune J. (1973) Coloration des chromosomes humains par l acridine orange, apr6s traitement par le 5-bromodeoxyuridine. C.R. Acad. Sci. 276, Eldridge F.E.A. (1974) A dicentric robertsonian translocation in a Dexter cow. J. Hered. 65, Ellsworth S.M., Paul R.S. & Bunch T.D. (1979) A 14/28 dicentric robertsonian translocation in a Holstein cow. Theriogenology 11, Ford C.E., Pollock D.L. & Gustavsson 1. (1980) Proc. 1st Int. Conf. Standardization of Banded Karyotypes of Domestic Animals, Reading, 2-6 August Hereditas 92, Frebling J., Foulley J.L., Berland H.M., Popescu C.P., Cribiu E.P. & Darre R. (1987) R6sultats de 1 enquete sur la frequence de la translocation 1/29 en race bovine Blonde d Aquitaine. Bull. Tech. C.R.Z. V. Theix, INRA 67, Gustavsson 1. (1969) Cytogenetics, distribution and phenotypic effects of a translocation in Swedish cattle. Hereditas 63, Gustavsson 1. & Rockborn G. (1964) Chromosome abnormality in 3 cases of lymphatic leukaemia. Nature 203, 990 Gustavsson L, Hageltorn M. & Zech L. (1976) Identification of the 1/29 translocation in the Swedish Red and White (SRB) cattle breed by utilization of new staining techniques. Hereditas 82, Logue D.N. & Harvey M.J.A. (1978) A 14/20 robertsonian translocation in Swiss Simmental cattle. Res. Vet. Sci. 25, 7-12 Popescu C.P. (1973) L h6t6rochromatine constitutive dans le caryotype bovin normal et anormal. Ann. Genet. 16, Popescu C.P. (1973) Nouvelles observations sur une fusion centrique chez Bos taurus L. Ann. Genet. S61. Anim. 5, Popescu C.P. (1977) Les anomalies chromosomiques des bovins (Bos tanrns L.). Etat actuel des connaissances. Ann. G6n6t. Sél. Anim. 9, Popescu C.P. (1977) A new type of robertsonian translocation in cattle. J. Hered. 68, Queinnec C., Darre R., Berland H.M. & Raynaud J.C. (1974) Etude de la translocation 1/29 dans la population bovine du Sud-Ouest de la France: Consequences zootechniques. Prem. Congr. Mond. Genet. Appl. Elev., 7-11 octobre 1974, Madrid, Editorial Garsi, Spain, pp Refsdal A.O. (1976) Low fertility in daughters of bulls with 1/29 translocation. Acta Vet. Scand. 17, Seabright M. (1971) A rapid banding technique for human chromosomes. Lancet 2, Sumner A.T. (1972) A simple technique for demonstrating centromeric heterochromatin. E!p. Cell Res. 75,