Breast Milk Sensor. Jane Doe DEMO_RR

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1 Breast Milk Sensor Jane Doe DEMO_RR

COVER LETTER Dear Ms. Doe, Your sample for the analysis arrived on 21/03/2017 in the laboratory and was evaluated according to the highest laboratory quality standards (ISO 15189).

2 COVER LETTER Dear Ms. Doe, Your sample for the analysis arrived on 21/03/2017 in the laboratory and was evaluated according to the highest laboratory quality standards (ISO 15189). The results were evaluated by two independent geneticists and molecular biologists and personally approved by me, the laboratory manager. After obtaining the results, your personal report was compiled. I hereby transmit the results to you in the format of your choice. I would like to thank you for your trust and I hope that you are satisfied with our service. We are always open for questions and suggestions, please do not hesitate to contact us. This is the only way we can continuously improve our services. I hope the analysis meets your expectations. Kind regards, Dr. Daniel Wallerstorfer, B.Sc. Laboratory director

3 Breast Milk Sensor Analysis report Jane Doe Date of birth: 01/04/1983 Order number DEMO_RR Powered by: This report contains personal, medical information and should be treated as confidential. Page 1 of 14

Prevention Early Detection Treatment BREAST MILK SENSOR The right nutrients for your baby Omega 3 in breast milk is important for the development of your child Omega-3 deficiency during development

4 Prevention Early Detection Treatment BREAST MILK SENSOR The right nutrients for your baby Omega 3 in breast milk is important for the development of your child Omega-3 deficiency during development can lead to an up to 6 point lower IQ score Omega-3 deficiency leads to slower eye development Supplementation and right nutrition can elevate Omega-3 levels Analysis of Omega-3 content in breast milk The right Omega-3 amount for your baby

5 BREAST MILK The role of breast milk in your baby's development During the first six months of life, your baby is completely dependent on a carefully balanced mix of nutrients in the breast milk. It contains everything your baby needs in either perfect, or close to perfect amounts to ensure fast development of your baby s body and brain. A number of environmental factors, however, influence the amount of the essential Omega 3 fatty acids in the mother s milk and numerous scientific studies have shown, that this can have a negative effect on the development of the child. This important building block is required to build new cell membranes in cells of all tissues of the body and especially in the development of neurons/brain cells of the brain. If the supply of Omega 3 fatty acids is too low during the time the brain develops and needs to build new brain cells, the overall development can be slowed and result in a generally lower intelligence quotient (IQ), lower Omega 3 content in red blood cells, impaired vision development and slower cognitive development. Even if the amount of Omega 3 is increased at later stages of life, the effects of slower development during the early stages of life will remain. This is why it is especially important for parents to ensure that the baby gets all of the necessary nutrients from the mother s milk during the time of breastfeeding. Testing mother s milk for Omega 3 content is an advanced and reliable way of determining the Omega 3 concentration and allows the mother to increase the amount of Omega 3 fatty acids through the use of nutritional supplements if necessary. Omega 3 and Development Omega three fatty acids have a number of roles in the development of your child: Building new cell membranes Omega 3 fatty acids are an essential building block for cell membranes. During the first 6 months of life your baby will only get Omega 3 from the mother s milk and it needs enough to build about new cells. Brain Development and IQ Especially brain cells require a lot of Omega 3 fatty acids to form. Only during the first 2 years of life new brain cells are formed in various regions of the brain (cerebellum, olfactory bulb, prefrontal cortex, and hippocampus). After the age of 2 the new formation stops and any development that was delayed during these 2 years can never be made up for later in life. Scientific studies have shown that Babies fed with high Omega 3 Milk can have up to 6 IQ points more. Page 3 of 14

6 Vision development Omega 3 also plays an important role in the development of the eyes. Studies have shown, that children with a higher Omega three content in their blood develop clearness of vision faster than babies with low Omega 3 content. Page 4 of 14

7 BREAST MILK SENSOR The Result - Month 3 Here you see the result of the Omega-3 measurement (DHA) of your sample. 3rd Trimester BREAST MILK BIRTH Month 1 Month 2 Month 3 analysis result TOO LOW MEDIUM OPTIMAL Month 3 Month 4 SOLID FOOD Your result: 0.58% Omega-3 (DHA) Month 5 Month 6 Month 7 Month 8 Month 9 The omega 3 content is too low and should be increased by supplementation with omega 3 capsules and change in nutrition. Possible values: 0% % (Too low) 0.32% % (moderate) 0.8% + (optimal) Month 10 Month 11 1st Birthday 1,5 Years old Page 5 of 14

8 THE RESULT The result You had a sample of the breast milk tested for Omega-3 content, which is essential for your babys development. The analysis of the latest sample came to the following conclusion: Omega-3 content too low! The Omega-3 content is too low in the breast milk sample you provided. This could lead to a slower development in your child and so you should try to increase the omega-3 content you your milk. Recommended daily supplementation: 588mg In principle any Omega-3 nutritional supplements will suffice, as long as it supplies you with the right dose of Omega-3 fatty acids per day. You have the option of having a personalized Omega-3 nutritional supplement created specifically based on your individual needs. This supplement has been created from the highest quality materials, ensures that you obtain the rights dosage per day and it is free of unhealthy heavy metals. You can order your personalised NutriMe-Omega-3 from our website at In addition it is recommended to increase your omega-3 content by including more fish into your diet. There are also some algae supplements containing DHA, which are suitable for vegetarians and vegans. Please remember your next testing interval in 2 months time to ensure that supplementation has had the required effect of increasing the omega 3 content or your breast milk. Page 6 of 14

Therefore, the right amount of omega-3 fatty acids in breast milk is of great importance.

9 NUTRIME NutriMe - Mother&Child Not too much and not too little, individually mixed for you! Omega 3 (more specifically DHA) is an essential micronutrient, necessary for the proper development of your child. Therefore, the right amount of omega-3 fatty acids in breast milk is of great importance. NutriMe Mother & Child is specifically created based on your analyses results, and ensures an adequate daily supply of omega-3 fatty acids. In addition to essential omega-3, many other important vitamins and minerals are included in NutriMe Mother & Child, to make sure your child is optimally supplied with essential micronutrients it needs during development. As your baby gets the necessary vitamins exclusively from breast milk during the first 6 months, it is particularly important that the mother is not lacking any micro nutrients. Sufficient nutrients for the mother means suficient nutrients for the developing child. Therefore NutriMe - Mother & Child has been specifically designed to be taken by the mother to ensure an adequate amount of nutrients in the breast milk. Constant nutrient supply throughout the day Since your body produces breast milk throughout the day, the micronutrients should be constantly available. This is not easy to accomplish, especially in the case of Vitamin C, as about 30 minutes after Vitamin C reaches the blood stream, half of it is already lost again. It is therefore important that micro-nutrients are released to the body in a slow but constant fashion. For this reason, NutriMe Mother & Child consists of slow-release micro-transporters. These are small capsules containing a specific ingredient, which is slowly released over a period of several hours. Optized absorption Certain micronutrients are blocking each other s absorption (for example, calcium and zinc), which means that products that contain both may have a poor absorption rate, or may even not be absorbed at all. Through the micro-transporter solution, interacting agents are placed in different transporter pellets and are released at different locations in the intestine. Thus, both substances can be effectively absorbed by the body. How to order: To place an order, go to: RECIPE CODE: DEMO_RR Page 7 of 14

CUSTOMER SERVICE Customer Service Questions or comments about our service? Our customer service team is happy to help with any enquiries, questions or problems.

10 CUSTOMER SERVICE Customer Service Questions or comments about our service? Our customer service team is happy to help with any enquiries, questions or problems. You can contact us in the following ways: Tel: +43 (0) Fax: +43 (0) Our team is looking forward to your call. Customer satisfaction is our first priority. If you are not fully satisfied with our service, please let us know. We will do our best to help find a satisfactory solution to your problem. GenoSense Diagnostics Saalachstrasse Salzburg Austria Page 8 of 14

REFERENCES References All our analyses and treatment recommendations are scientifically validated. Here are some of the relevant literature references for your information. Wajner M., Coelh DdM.

11 REFERENCES References All our analyses and treatment recommendations are scientifically validated. Here are some of the relevant literature references for your information. Wajner M., Coelh DdM., et al., ( 2009) : Selective screening for organic acidemias by urine organic acid GC MS analysis in Brazil: Fifteen-year experience. Clinica Chimica Acta 400: Verma IC, Bijarnia S and Puri RD. (2006) Burden of genetic Disorders in India. In Preventive genetics (Ed) Gogates. pp Jaypee Brothers Medical Publishers (P) Ltd. Sharma S., Kumar P., et al., (2008): Approach to Inborn errors of Metabolism Presenting in the neonate. The Indian Journal of pediatrics. 75( 3);271 Database for Inborn Errors of Metabolism in the Indian State of AP Noninvasive human metabolome analysis for differential diagnosis of inborn errors of metabolism; Tomiko Kuhara; doi: /j.jchromb A new chemical diagnostic method for inborn errors of metabolism by mass spectrometry- rapid, practical and simulateneous urinary metabolites analysis; I. Matsumoto and T. Kuhara; Mass Spectrometry Reviews, , Selective screening for organic acidemias by urine organic acid GC MS analysis in Brazil: Fifteenyear experience; Moacir Wajner et al.; Clinica Chimica Acta 400 (2009) Dobbing J, Sands J. Quantitative growth and development of human brain. Arch Dis Child. 1973;48(10): Martinez M. Tissue levels of polyunsaturated fatty acids during early human development. J Pediatr. 1992;120(4 Pt 2):S129-S138. Makrides M, Neumann MA, Byard RW, Simmer K, Gibson RA. Fatty acid composition of brain, retina, and erythrocytes in breast- and formula-fed infants. Am J Clin Nutr. 1994;60:(2) Haggarty P, Page K, Abramovich DR, Ashton J, Brown D. Long-chain polyunsaturated fatty acid transport across the perfused human placenta. Placenta. 1997;18(8): Auestad N, Halter R, Hall RT, et al. Growth and development in term infants fed long-chain polyunsaturated fatty acids: A doublemasked, randomized, parallel, prospective, multivariate study. Pediatrics. 2001;108(2): Birch EE, Hoffman DR, Uauy R, Birch DG, Prestidge C. Visual acuity and the essentiality of docosahexaenoic acid and arachidonic acid in the diet of term infants. Pediatr Res. 1998;44(2): Helland IB, Smith L, Saarem K, Saugstad OD, Drevon CA. Maternal supplementation with very-long-chain n-3 fatty acids during pregnancy and lactation augments children's IQ at 4 years of age. Pediatrics. 2003;111(1):e39-e44. The British Nutrition Foundation. Unsaturated fatty acids: Nutritional and physiological significance. London: Chapman & Hall; 1992; Food and Agricultural Organization of the United Nations/World Health Organization Joint Expert Consultation. Lipids in early development. In: Fats and oils in human nutrition. FAO Food and Nutri Pap. 1994;57: Simopoulos AP, Leaf A, and Salem N Jr. Workshop on the essentiality of and recommended dietary intakes for omega-6 and omega-3 fatty acids. J Am Coll Nutr. 1999;18(5): Hoffman DR, Wheaton DKH, James KJ, et al. Docosahexaenoic acid in red blood cells of term infants receiving two levels of long-chain polyunsaturated fatty acids. J Pediatr Gastroenterol Nutr. Page 9 of 14

12 2006;42(3): Auestad N, Montalto MB, Hall RT, et al. Visual acuity, erythrocyte fatty acid composition, and growth in term infants fed formulas with long chain polyunsaturated fatty acids for one year. Ross Pediatric Lipid Study. Pediatr Res. 1997;41(1):1-10. Hoffman DR, Birch EE, CastanedaYS, et al. Dietary docosahexanoic acid (DHA) and visual maturation in the post-weaning term infant. Invest Opthamol Vis Sci. 2001;42:S Hoffman DR, Birch EE, Castañeda YS, et al. Visual function in breast-fed term infants weaned to formula with or without long-chain polyunsaturates at 4 to 6 months: A randomized clinical trial. J Pediatr. 2003;142(6): Innis SM, Gilley J, Werker J. Are human milk long-chain polyunsaturated fatty acids related to visual and neural development in breast-fed term infants? J Pediatr. 2001;139(4): Birch EE, Garfield S, Castañeda Y, et al. Visual acuity and cognitive outcomes at 4 years of age in a double-blind, randomized trial of longchain polyunsaturated fatty acid-supplemented infant formula. Early Human Dev. 2007;83(5): U.S. Department of Agriculture. Agricultural Research Service. Page 10 of 14

CERTIFICATIONS Certifications The Novogenia laboratory is one of the most modern and automated laboratories in

standards. The various fields of business are certified separately to the highest standards.

interpretation of genetic analyses Certified through analysis in our ISO 15189 certified laboratory Scientific

13 CERTIFICATIONS Certifications The Novogenia laboratory is one of the most modern and automated laboratories in Europe, and has numerous certifications and quality assurance systems that meet and exceed international standards. The various fields of business are certified separately to the highest standards. Analysis for Lifestlye-purposes Certified through analysis in our ISO certified laboratory Medical interpretation of genetic analyses Certified through analysis in our ISO certified laboratory Scientific release of analysis results Company and office Licensed for medical genetic analyses by the Austrian government Certified through ISO 9001 Page 11 of 14

TECHNICAL DETAILS Technical details Address Analysis method Musterstrasse 27 1234Musterstadt AUSTRIA Gas Chromatography and Mass Spectrometry Detection rate ~>99% Ordernumber DEMO_RR Reason for

14 TECHNICAL DETAILS Technical details Address Analysis method Musterstrasse Musterstadt AUSTRIA Gas Chromatography and Mass Spectrometry Detection rate ~>99% Ordernumber DEMO_RR Reason for Analysis Business Analysis for prevention or early detection and treatment of inborn errors of metabolism GenoSense Diagnostics Saalachstrasse Salzburg Austria Analysing laboratory Novogenia GmbH Saalachstrasse Salzburg, Austria Laboratory Head Dr. Daniel Wallerstorfer Bsc. Page 12 of 14

15 NOTES:

17 Breast Milk Sensor Jane Doe DEMO_RR

Maternal and Infant Nutrition Briefs

Maternal and Infant Nutrition Briefs A research-based newsletter prepared by the University of California for professionals interested in maternal and infant nutrition March/April 2003 New Guidelines on