T-2 and HT-2 in bright coloured and dark oats. Tests accredited under the German DAR accreditation scheme which complies to ISO 17025
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1 T-2 and HT-2 in bright coloured and dark oats T2 toxin - 87 µg/kg, HT-2 toxin 290 µg/kg T2 toxin - <10 µg/kg, HT-2 toxin 22 µg/kg Tests accredited under the German DAR accreditation scheme which complies to ISO
2 T-2 and HT-2 toxins in oat products oat products from
3 3 H. & J. Brüggen KG Lübeck, 2006
4 4 H. & J. Brüggen KG Lübeck, 2006
5 Assessment of maximum quantity p-tdi: 0,06 µg T-2 and HT-2/kg body weight (safety factor 500, due to less data available and low difference between LOAEL and NOAEL) (SCF, 30 May 2001) maximum quantity p TDI body weight in food µg/kg: daily consumption maximum quantity: (at-risk group: child, high consumer) 0,06µg / kg bw 16,15 0,0643kg kg = 15 µg / kg with current data estimated maximum quantity is not feasible!!! 5
6 Further steps Toxicology - Assessment of the toxicology study in general - Assessment of the availability of T2 /HT2 from oats - Assessment of the safety factor of 500 Establishment and validation of an analytical method Collection of further data Development of guidelines for growers and processors 6
7 Conclusions As an industry we have an extensive programme of ongoing work which is being undertaken across Europe and more time is needed to obtain, assess and analyse the data Climate is one of the main influencing factors which leads to great variation in disease incidence and resultant Mycotoxin levels BUT it is not necessarily what you would expect 7
8 Conclusions We need time to understand the interactions between different Mycotoxins and the influences on them and until more work has been undertaken, which we as an industry are already actively pursuing, the management of this will remain difficult 8
9 Thank you for your attention 9
10 Literature B a n a s ia k U, H e s e h e r H, S ie k e C, S o m m e rfe ld C, V o h m a n n C ( ). A b s c h ä tz u n g der Aufnahm e von Pflanzenschutzm ittel-rückständen in der Nahrung m it neuen Verzehrsm engen für Kinder. Bundesgesundheitsbl-G esundheitsforsch- G esundheitsschutz. 48, Biörklund M., van Rees A., M ensink RP., O nning G. (2005). Changes in serum lipids and postprandial glucose and insulin concentrations after consum ption of beverages with beta-glucans from oats or barley: a random ised dose-controlled trial. European j o u rn a l o f c lin ic a l n u tritio n, Vol. 59 (11), G re g o ry, J.R., F o s te r, K., T y le r, H. & W is e m a n, M. ( ) Dietary and Nutritional S u r v e y o f B ritis h A d u lts, London: Her M ajesty s Stationery O ffice. G re g o ry, J.R., C o llin s, D.L, D a v ie s, P.S.W., H u g h e s, J.M. & C la rk e, P.C. ( ) National Diet and Nutrition Survey; Children Aged 11/2 to 41/2 Years. Volum e 1: Report of the Diet and Nutrition Survey, London: Her M ajesty s Stationery O ffice. HG CA, Q uaker O ats and Harper Adam s PhD studentship Kersting, M. (2001). Ernährung des gesunden Säuglings M onatsschr. Kinderheilkd., 149, Langseth, W. (2000). A survey on the occurrence of m ycotoxins in cereals on the Norwegian m arket. Report from National Veterinary Institute, Norway Norkost (1997) G EM S/Food spreadsheet. S c ie n tific C o m m itte e o n F o o d. ( a ). O p in io n o n Fusarium T o x in s, p a rt 5 : T -2 to x in a n d H T -2 to x in (a d o p te d b y th e S C F o n 3 0 M a y ) m /Food/fs/sc/scf/out88_en.pdf S c ie n tific C o m m itte e o n F o o d. ( ). O p in io n o n Fusarium Toxins, part 6: G roup evaluation of T-2 toxin, HT-2 toxin, nivalenol and deoxynivalenol (adopted by the SCF on 26 February 2002.) m /Food/fs/sc/scf/index_en.pdf Schönberger, H. und U. Kropf (2000). Hafer. Lehrbuch des Pflanzenbaus. Band 2, Verlag Th. M ann, G elsenkirchen, W elch W. The oat crop. Chapm an and Hall, 1995 W ürsch P., Pi-Sunyer FX. (1997). The role of viscous soluble fiber in the m etabolic control of diabetes. A review with special em phasis on cereals rich in beta-glucan. Diabetes care, Vol. 20 (11), Zechner E. (2001). Züchtung von Q ualitätshafer für die Nahrungsm ittelproduktion, A rbeitsgem einschaft landwirtschaftlicher Versuchsanstalten w w w.d e fra.g o v.u k, w w w.celiac.ca, 2006 w w w.fa o.o rg,
11 Data Sources Finland: Finnish Food and Drink Industries Federation, FIN Helsinki; Peter Kölln KGaA, D Elmshorn; H. & J. Brüggen KG, D Lübeck Sweden: Peter Kölln KGaA, D Elmshorn; H. & J. Brüggen KG, D Lübeck; Germany: UK: Peter Kölln KGaA, D Elmshorn; H. & J. Brüggen KG, D Lübeck; BOBMA, The British Oat and Barley Millers Association UK : Harper Adams University College 11
12 Analytical Methods GC/MS HPLC/MS HPLC flourescence detection LC-MS/MS 12
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