Biobank Construction after the Earthquake and Tsunami Disaster in Japan
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1 Biobank Construction after the Earthquake and Tsunami Disaster in Japan Naoko Minegishi 1, Hisaaki Kudo 1, Ichiko Nishijima 1, Atsushi Hozawa 1, Shinichi Kuriyama 1, Kozo Tanno 2, Ryohei Sasaki 2, Takako Takai 1, Soichi Ogishima 1, Hideyasu Kiyomoto 1, Tsuyoshi Hachiya 2, Yu Shiwa 2, Atsushi Shimizu 2, Mamoru Satoh 2, Jiro Hitomi 2, Nobuo Yaegashi 1, Kenji Sobue 2, Masayuki Yamamoto 1 1. Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan. 2. Iwate Tohoku Medical Megabank Organization, Iwate Medical University, Morioka, Japan.
2 The Great East Japan Earthquake and Tsunami, March 11, 2011 Sendai, 2011 Natori, 2013 Earthquake: Mw 9.0 Tsunami: Max 40 m High Killed or Missing People: 18,539 Disaster Related Death: 3,076 Destroyed Houses: 398,770 The Victims have not Returned to Their Normal Lives.
3 Current and Future Health Problems in the Devastated Area Psychosocial Problems Family Death Loss or Change Jobs Changes in Household Economy Debts Changes in Home Environment Moving from Affected Areas Life Style Changes Withdrawal from Society Living Alone in Temporary Housing Lack of Exercise Nutritional Imbalance Increase of Life-Style Diseases? Psychiatric Diseases? Cognition Disorders? Cancers? Orthopedic Problems? Children s Diseases? Developmental Problems?
4 Prospective Cohort Study in Tsunami-Devastated Area Iwate Medical Univ. Disaster-Related Risk of Diseases Health Problems after Disaster Facilitate Personalized Medicine and Healthcare Pacific Coast Areas of Iwate Prefecture 30,000 people Three Generation Cohort (Miyagi) Miyagi Prefecture 120,000 people (Including Three Generation Cohort Study) Fukushima Pref. Studies Related to Nuclear Medicine Tohoku Univ. Mother 20,000 Sibling 5,000 (Saliva) Grandparents 15,000 Father 10,000 Newborn 20,000
5 5 Governance of Biobank In Tohoku Medical Megabank Organization Tohoku University Iwate Medical University National Working Groups for Ethical and Legal Issues, for Genome-Cohort Study, for Genome-Omix Research, for Regional Medical Service Steering Committee ELSI Committee Access Committee Data / Samples Broad Consent Withdrawal Anonymized Data Storage Disclosure Data Access Data / Samples Institutional Review Board Researchers (Profit/ Non-Profit) Return Results Research Results
6 Sample and Health Information Collection Total Blood 34ml+Urine 13 ml of Blood Biochemical Test, Blood Test, HbA1c 7ml (EDTA) 9ml (Plain) 5ml (Heparin) Urine Plasma/Buffy Coat (for DNA Extraction) Serum Mononuclear Cells Physiological Exam., MRI (20,000 people) Questionnaire (Japanese-Cohort Standard) Age, Sex, Area, Job, Diet, Sleep, Exercise, Alcohol, Smoking, History, Prescription, Gynecologic Problems, Mental Health Psychosocial Factors Disaster-Related Questions (Damage, Life-Style Change) Consent Form
7 Participants Collection, Transportation and Anonymization Miyagi Pref. Obstetrician s Office Health Check-Up Sites (Governments- Organized) Biobank Sample Preparation/Storage (Tohoku Univ.) Center Anonymous Database. Iwate Pref. Health Check-Up Sites (Governments- Organized) Clinical Laboratory Satellite Questionnaires Anonymization Cohort Database The Biobank Receives Samples within 36 Hours from Collection.
8 Sample Preparation and Storage Register Preparation (with Robotics) Storage Blood (EDTA) Centrifuge EDTA Plasma 4 Tubes Buffy Coat 2 Tubes -80 DNA Extraction (Buffy Coat of 5 ml Blood) ± 16.0 ng/µl (Picogreen, 10,000 samples) Blood(Plain) Centrifuge 66.2 ± 20.7 ng/µl (Nanodrop, 742 samples) OD280/260=1.82 ± 0.09 (Nanodrop, 742 samples) Serum 3 4 Tubes -80 Urine 3 Tubes -80 Blood (Heparin) (Cord Blood) MNC Wash 2 Tubes 9.2 ± 4.2 *10 6 / 5ml Program Freezing EBV-LCL (100 people ) Activated T-Cell (80 people) Laboratory Information Management System (LIMS) Liquid N2 (Vapor Phase)
9 Storage Samples in the First Year Participants 30,000 (3G Cohort 5,000) Storage 2D Tubes 450,000 DNA 17,000 (May, 2014)
10 Tohoku Medical Megabank Projects Healthy Volunteer Specimen Data Analysis Storage ICT Expected Results Regional Population Cohort Study Three Generation Cohort Study Plasma Serum Mononuclear Cells (Peripheral and Cord Blood) Urine DNA (Blood, Saliva) Whole Genome Analysis Transcriptome Epigenome Proteome Metabolome Robotics DNA Purification Sample Preparation Storage LIMS (Laboratory Information Management System) Life Science Biomarker Personalized Medicine/Heal thcare Questionnaire Health Check Data Follow Up Medical Information System Public Health In the Region Affected by the Disaster For the Health of Stricken People To Prepare for Next Disaster Thank You for Your Kind Attention
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