TILDA The Irish Longitudinal Study on Ageing. Ann Hever
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1 TILDA The Irish Longitudinal Study on Ageing Ann Hever R&D Programme Manager
2 TILDA Overview Study Design Data Collected Biological Data and Plans
3 Where are we now Wave 1 Wave 2 Wave 3 Wave
4 Bio samples Bio samples Wave 1 Wave 2 Wave 3 Wave
5 Sampling design Established 2006, data commenced 2009 No extant study to recruit from; 25,600 households screened to person aged % of those eligible were recruited sample; equal probability of
6 8,
7 Follow up Every 2 years
8 TILDA represents 1:140 aged 50+ Age Group < 50 (partners) Number , , ,
9 Three modes of data collec#on Face- to- face personal interview in the s home, conducted by trained interviewers Self- comple#on ques#onnaire booklet given to by interviewer to complete in their and post back to TILDA Health assessment at dedicated centre on the campus of Trinity College Dublin, or a modified version in the par@cipant's home, administered by trained nurses
10 Inclusion criteria At Wave 1, only those who were non- demented and capable of providing informed consent were invited to in the study At subsequent waves all are followed up regardless of developing or other moving to Proxy interview is conducted with nominated proxy if is not able to do the interview Proxy also asked to conduct End- of- Life interview
11 Wave Wave Wave Wave Wave Wave CAPI: computer- assisted personal interview; SCQ: self- comple@on ques@onnaire; Health: TILDA health assessment
12 TILDA CAPI (approx 1.5 hours) Economic Health Social Employment Job history Planning for Sources of Income House ownership Other assets Healthcare Literacy (financial, health) Physical health health Mental health Behavioural health Household circumstances Demographics Transfers to Children Transfers to Parents Social connectedness of daily living and helpers Transport
13 Biomarker measurements Cogni#ve Mini- Mental State (MMSE) Montreal Assessment (MOCA) Sustained to Response Task (SART) Picture memory test Adult Reading Test (NART) Visual reasoning Colour trails test Depressive symptoms State anxiety Cardiovascular Pulse wave velocity Sieng & standing blood pressure Phasic blood pressure Heart rate variability Cerebral perfusion (NIRS) Mobility Timed Up- and- Go Repeated chair stands GAITRite assessment Usual speed Maximum speed Dual task Vision Visual acuity Contrast Macular pigment density Re#nal photograph (SHAMS) Anthropometric / other Height Weight Waist & hip circumference Grip strength Heel ultrasound Dental health Blood samples Hair samples Brain MRI Accelerometry Diet (SCQ)
14 Blood samples 25ml collected Wave 1: 5,800 samples Wave 3: 5,300 repeat samples 1 X Lithium Heparin Blood Tubes (5ml blood volume) 2 X EDTA Blood Tubes (10ml blood volume)
15 Blood samples in Wave 3 1 X PAXgene (RNA) Blood Tubes (2ml blood volume) 1000 samples only
16 TILDA Blood Collec#on Model COLLECTION Trinity College Dublin (~4,800 samples) Homes (~1,000 samples)
17 COLLECTION Trinity College Dublin (~4,800 samples) Homes (~1,000 samples) TRANSPORTATION (DX Ireland Ltd) COURIER 1: TCD COURIER 3: Countrywide
18 COLLECTION Trinity College Dublin (~4,800 samples) Homes (~1,000 samples) TRANSPORTATION (DX Ireland Ltd) COURIER 1: TCD COURIER 3: Countrywide PROCESSING & ANALYSIS (Trinity Biobank & St. James Hospital) IMMEDIATE ANALYSIS LIPID PROFILE CENTRAL LABORATORY CENTRIFUGE AND ALIQUOT DATA TRANSFER TO TILDA SHORT- TERM STORAGE (- 20 O C) Processed within 48 hours
19 COLLECTION Trinity College Dublin (~4,800 samples) Homes (~1,000 samples) TRANSPORTATION (DX Ireland Ltd) COURIER 1: TCD COURIER 3: Countrywide PROCESSING & ANALYSIS (Trinity Biobank & St. James Hospital) IMMEDIATE ANALYSIS LIPID PROFILE CENTRAL LABORATORY CENTRIFUGE AND ALIQUOT DATA TRANSFER TO TILDA SHORT- TERM STORAGE (- 20 O C) BIO- BANKING (2 loca@ons St. James s Hospital and Trinity Campus) - 80 o C freezers at 2 loca#ons Temp. Monitoring & Biobanking
20 Biomarkers Analysed to March 2016 General func#on/ Cardiovascular Lipid profile cholesterol Folate Vitamin B12 Vitamin D Vitamin E CRP
21 Biomarkers Analysed to March 2016 General func#on/ Cardiovascular Lipid profile cholesterol Folate Vitamin B12 Vitamin D Vitamin E CRP Renal func#on Crea@nine Cysta@n C
22 Biomarkers Analysed to March 2016 General func#on/ Cardiovascular Lipid profile cholesterol Folate Vitamin B12 Vitamin D Vitamin E CRP Renal func#on Kidney func#on Crea@nine Cysta@n C HbA1c (frozen buffy coat)
23 Biomarkers Analysed to March 2016 General func#on/ Cardiovascular Lipid profile cholesterol Folate Vitamin B12 Vitamin D Vitamin E CRP Renal func#on Kidney func#on Retainal func#on Crea@nine Cysta@n C HbA1c (frozen buffy coat) Lutein Zeaxanthin Lycopene Alpha- carotene Beta- carotene Beta- cryptoxanthin
24 DNA 7 SNPs associated with age- related macular degenera#on CFH ARMS CFB SKIV2L C3 TIRAP Claudin 5
25 Hair Hormones Testosterone Beta- estradiol Progesterone Melatonin
26 MRI Scans minute protocol, 7 different scans 3T MRI scanner equipped with a 32- channel head coil for op@mal signal- to- noise characteris@cs.
27 MRI Scan a. High resolution 2D T2-weighted sequence b. High resolution 3D T1-weighted volumetric scan (IR-SPGR) c. Fluid Attenuation Inversion Recovery (FLAIR) d. Diffusion Weighted Imaging (DWI) 4 b-values acquisition for apparent diffusion coefficient (ADC) quantification e. Diffusion Tensor Imaging (DTI) 61 directions, b-value 1200s/mm2 f. Pseudo-Continuous Arterial Spin Labelling (pcasl) Single shot echo-planar imaging (EPI) sequence with background suppression g. fmri Blood Oxygenation Level Dependent scan (BOLD)
28 Wave 5 Biological Samples (2018/19) Blood Hair RNA on full cohort Faecal samples on full cohort Urine? Repeat Brain MRI
29 Data access Publically available (biomarkers not yet deposited) Wave 1 and wave 2 data currently available from: Irish Social Science Data Archive (ISSDA): Interuniversity Consor#um for Poli#cal and Social Research (ICPSR): Wave 3 deposited 2016
30 Data access Full dataset (Wave 3 and biomarkers) Access through TILDA servers on Trinity College Dublin campus Restricted access to approved projects only Applica@on to TILDA for access
31 Thank you! Dr. Ann Hever
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