INDIGENOUS TRADITIONAL KNOWLEDGE (TK) IN PENINSULAR MALAYSIA: PHYTOCHEMICAL & BIOACTIVITY SCREENING
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1 INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA INDIGENOUS TRADITIONAL KNOWLEDGE (TK) IN PENINSULAR MALAYSIA: PHYTOCHEMICAL & BIOACTIVITY SCREENING BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
2 Research Activities TK awareness workshop Prior informed consent (PIC) Socio-economic survey Documentation & specimen collection training Sampel collection Phytochemical & bioactivity studies of selected species (Semelai, RPS Iskandar, Bera, Pahang) BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
3 TRADITIONAL KNOWLEDGE (TK) DOCUMENTATION PROCESS Selection of community & tentative sites Rapid Rural Appraisal (RRA) TK Awareness Workshop -ve +ve Documentation in TK Database (BRAHMS) & TK website Capacity Building Workshops (PIC 2) Household survey (PIC 1) Phase 2 Phase 1 BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
4 Selection of species Criteria: Commonly used/popular among people in the community Medicinal uses - not publicly available Traditionally used for fever, infection, diabetes, personal care etc. Part used (leaf, bark, stem) Availability (enough sample: 3-5kg) BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
5 Phytochemical & bioactivities screening Traditional knowledge (TK) Sample collection Sample preparation Extraction Phytochemical screening Bioactivity screening Phytochemical & bioactivity profile
6 Sampel Location Sub-ethnic Date No species 1 RPS Iskandar, Bera, Pahang 2 RPS Air Banum, Gerik, Perak Semelai 2-4Feb 2010 Jahai/Temiar Jul 2010 No extract No essential oil Ulu Geroh, Gopeng, Perak 4 Kg. Pulau Kempas, Kuala Langat, Selangor 5 Kg Paya Mendoi, Kuala Krau, Pahang 6 Kg Bangkong, Pekan, Pahang Semai Mei 2011 Temuan Ogos 2011 Jahut Jakun 4-6 Okt Nov
7 Sampel Location Sub-ethnic Date No species 7 Kg Selangi, Kota Tinggi, Johor 8 Kg Sg Berua, Kuala Berang, Terengganu 9 Kg. Aring, Gua Musang, Kelantan 10 Kg Enggang, Lancang, Pahang Kanaq May 2012 Semoq Beri 3-5 Jul 2012 Bateq Aug 2012 Che Wong Sept 2012 No extract No essential oil BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
8 Sample collection
9 Sample preparation
10 Sample preparation
11 Sample processing drying grinding Extract Evaporation Soaking (methanol)
12 Phytochemical profile To screen chemicals content: Saponins Flavonoids Tannin Triterpenes Sterols Essential oil analysis BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
13 Bioactivity profile To validate & assess the potential as therapeutic / nutraceutical / cosmeceutical agents Activity Assays 1 Antioxidant DPPH Radical Scavenging Assay 2 Antiinflammatory Xanthine Oxidase enzyme inhibition assay Hyaluronidase enzyme inhibition assay TPA-induced ear oedema 3 Antimicrobial Anti-Methicillin resistant S.aureus (anti- MRSA) 4 Antidiabetic Alpha amylase inhibition assay Alpha glucosidase inhibition assay 5 Cytotoxicity (safety) Liver cell line Kidney cell line
14 Bioactivity profile RPS Iskandar, Bera, Pahang Species Extract No of samples Bioactivity Species Extract Antioxidant Antimicrobial 7 10 Antiinflammatory 7 12 Antidiabetic 8 12 Cytotixicity (Safety) 9 15
15 Bioactivity profile No of species 3 2 activities 3 activities activities
16 Bioactivity profile Species Uses Part used Part extract AO AI IM AD CT 1 TSBR_ X 2 TSBR_ Y stomach ache, hyperten sion, diabetes, post partum fly repellent bark branch DT10/693 L L L H NT bark DT10/694 H H H H NT leaf leaf DT10/682 H H H H NT branch DT10/683 H H H H NT AO Antioxidant, AI Antiinflammatory, IM Antimicrobial, AD Antidiabetic, CT - Cytotoxicity (safety) H high activity, L low activity, NT - nontoxic
17 Phytochemical profiles Extract code Part Sa Fla Ta Tri Ste DT10/682 leaf 2 cm H DT10/683 branch 0.5 cm H 2 1+ DT10/694 bark 1 cm 1+ C 1 BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
18 Discussion/Meeting with the community to inform the results gain from the lab analysis (19 Jan 2012) 2 nd Phase Study Project Development of product prototype from TSBR087 based on traditional knowledge of Semelai subethnic (NRE) 2012/2013
19 Conclusions Preserve & maintain - TK Empower local orang asli Potential product development Possible benefit-sharing with the communities BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
20 Team members NRE FRIM Norini Haron & Rasadah Mat Ali Socio-economic: Lim Hin Fui, Norshakila Yusof, Intan Nurulhani Baharuddin Phytochemistry: Adiana Mohd Adib, Nuziah Hashim, Ling Sui Kiong Essential oil: Nor Azah Mohd Ali, Mailina Jamil Bioactivity: Nik Musaadah Mustapha, Mastura Mohtar, Fadzureena Jamaludin, Normaizira Hamidi, Vimala Subramaniam, Rohana Sahdan, Mohamad Jemain Mohamad Ridhwan, Mazurah Mohamed Isa Ethnobotany: Tan Ai Lee, Nurul Husna Zaidi, Chang Yu Shyun, Zainon Abu Samah, Nurnida Mohd Kamal, In Collaboration with Jawatankuasa Penasihat Pengetahuan Tradisi Semelai Pos Iskandar Tasek Bera, Pahang and Jawatankuasa Pengetahuan Tradisi RPS Banum, Gerik, Perak
21 BrandLaureate Best Brand Awards ISO Certified 5S INSTITUT PENYELIDIKAN PERHUTANAN MALAYSIA FOREST RESEARCH INSTITUTE MALAYSIA
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