DIFFERENTIAL SOMATIC CELL COUNT A NEW BIOMARKER FOR MASTITIS SCREENING
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1 SEMINARIO AIA Laboratori, 24 January 2017 Dedicated Analytical Solutions DIFFERENTIAL SOMATIC CELL COUNT A NEW BIOMARKER FOR MASTITIS SCREENING Dr. Daniel Schwarz, FOSS, Denmark
2 CENTRAL MILK TESTING Solutions in operation in 80+ countries 3,000+ MilkoScan sold (7 generations) 3,000+ Fossomatic sold (7 generations) 1,200+ BactoScan sold (3 generations) 2 Solutions in compliance with international and national standards (e.g. IDF/ISO, EU, FDA/NCIMS)
3 DHI TESTING CHAIN Milk-testing laboratory 3 Dairy farm
4 THE NEW COMBIFOSS 7 4
5 7 TH GENERATION COMBIFOSS SCC Fat Protein Lactose Solids Urea Citric Acid Freezing Point Depression Free Fatty Acids Casein ph Fatty acid profile Ketosis Adulteration Differential SCC CF 215 CF 360 CF 4000 CF 5000 CF 6000 CF FT+ CF s 1980s 1980s 1990s 2000s 2010s
6 7 TH GENERATION COMBIFOSS 19 parameters in DONE 6sec. Citric Acid Urea Solids Non Fat Total Solids Lactose Casein Crude Protein True Protein Fat Diff. Somatic Cell Count Somatic Cell Count Adulteration BHB Ketosis Acetone Ketosis Fatty Acid Profile 2 Fatty Acid profile 1 PH FPD Free Fatty Acids 6
7 Differential Somatic Cell Count and Mastitis Screening 7
8 CLINICAL MASTITIS 8
9 SUBCLINICAL MASTITIS 9
10 SCC Dedicated Analytical Solutions DEVELOPMENT OF UDDER HEALTH 600,000 cells/ml 200,000 cells/ml e.g. Sampimon et al., 2005 Time
11 ECONOMICAL LOSSES Dedicated Analytical Solutions 11
12 CELLS IN MILK 1. Lymphocytes 2. Polymorphonuclear neutrophils (PMN) 3. Macrophages Microscope spot, milk slide 12 Sordillo and Nickerson, 1988; Nickerson, 1989; Paape et al., 2002; Oviedo-Boyso et al., 2007
13 SCC Dedicated Analytical Solutions MASTITIS CASCADE Macrophages PMN 13 Macrophages Time Macrophages
14 THE NEW FOSSOMATIC 7 DC 14
15 New chemistry THE FOSS DSCC METHOD New measuring unit FL2 Laser Incubation unit SSC FL1 15 Patented technology
16 FL 2 FL 2 Dedicated Analytical Solutions THE FOSS DSCC METHOD Analysis of SCC Analysis of DSCC SCC (cells/ml) macrophages 16 FL 1 SSC information used supportively for determination of SCC FL 1 DSCC (%) PMN + lymphocytes
17 DSCC (%), fluorescence microscopy Dedicated Analytical Solutions SPECIFICITY OF THE DSCC METHOD n = 113 r = DSCC (%), FOSS DSCC method
18 FL 2 Dedicated Analytical Solutions ACCURACY OF THE DSCC METHOD Analysis of DSCC 94% macrophages DSCC (%) 83% PMN + lymphocytes 18 FL 1
19 SCC ( 1,000 cells/ml), Fossomatic FC Dedicated Analytical Solutions SCC REFERENCE n = r = SCC ( 1,000 cells/ml), FOSS DSCC method
20 DIFFERENTIAL SCC (DSCC) New business opportunities 20
21 ENHANCED TOOL FOR MASTITIS MONITORING Targeted selection of suspicious cows Current state, SCC: Prospective state, SCC and DSCC: Low SCC High SCC Low SCC High SCC Bacteriological examination Bacteriological examination Treatment strategy Treatment strategy 21 Healthy Infected
22 IMPROVED BACTERIOLOGICAL EXAMINATION Targeted selection Better interpretation 22
23 USE OF ANTIBIOTICS AT DRY OFF Improved decision tool 23
24 DSCC (%) Titolo asse Dedicated Analytical Solutions EXAMPLE FOR DSCC DATASET normal normal likely to be infected likely to be infected active.. inactive inflammatory response SCC (x 1,000 cells/ml)
25 DSCC RESEARCH IN PROGRESS 3-year projects in Microbiological testing Development of guidelines 25
26 MORE DSCC RESEARCH PROJECTS Project Objective Status University 1 DSCC before, during, and after artificial intramammary infection of cows under controlled conditions Data analysis University 2 Value of DSCC for selective dry cow therapy Data analysis DHI laboratory, North America DHI laboratory, North America DHI laboratory, Europe DHI laboratory & universities, Europe 26 Investigation of correlation between DSCC and udder health status in a longitudinal study DSCC for enhanced analysis of udder health in fresh lactating cows DSCC as parameter for improved microbiological testing DSCC as indicator for prediction of the recovery phase after mastitis Kick-off: March 2017 Kick-off: March 2017 Kick-off: May 2017 Kick-off: October 2017
27 A MESSAGE TO TAKE HOME First high-throughput analyser for DSCC DSCC (%) = PMN + lymphocytes More information on actual inflammatory status New possibilities to further tackle
DSCC A NEW BIOMARKER FOR MASTITIS SCREENING -- AND AN UPDATE ON KETOSIS SCREENING
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