DSCC A NEW BIOMARKER FOR MASTITIS SCREENING -- AND AN UPDATE ON KETOSIS SCREENING

Transcription

1 DSCC A NEW BIOMARKER FOR MASTITIS SCREENING -- AND AN UPDATE ON KETOSIS SCREENING Dr. Daniel Schwarz, FOSS, Denmark 03 November 2016 DEDICATED TO THE FOOD & AGRICULTURAL INDUSTRIES 2 1

2 CENTRAL MILK TESTING 3 THE NEW COMBIFOSS 7 4 2

3 CATCH THE NEXT BIG WAVE 19 parameters in 6 seconds from same sample, including differential somatic cell count + More results at less cost with 7 th generation CombiFoss technology Higher proficiency and less man hours with the latest in instrument management software 5 7 TH GENERATION COMBIFOSS SCC Fat Protein Lactose Solids Urea Citric Acid Freezing Point Depression Free Fatty Acids Casein ph Fatty acid profile Ketosis Adulteration Differential SCC CF 215 CF 360 CF 4000 CF 5000 CF 6000 CF FT+ CF s 1980s 1980s 1990s 2000s 2010s

4 CLINICAL MASTITIS SUBCLINICAL MASTITIS 7 8 4

5 ECONOMICAL LOSSES 9 CELLS IN MILK 1. Lymphocytes 2. Polymorphonuclear neutrophils (PMN) 3. Macrophages Microscope spot, milk slide 10 Sordillo and Nickerson, 1988; Nickerson, 1989; Paape et al., 2002; Oviedo-Boyso et al.,

6 MASTITIS CASCADE Macrophages SCC PMN 11 Macrophages Time Macrophages New chemistry THE FOSS DSCC METHOD New measuring unit FL2 Laser Incubation unit SSC FL1 12 Patented technology 6

7 THE FOSS DSCC METHOD Analysis of SCC Analysis of DSCC SCC (cells/ml) FL 2 FL 2 macrophages 13 FL 1 SSC information used supportively for determination of SCC FL 1 DSCC (%) PMN + lymphocytes SPECIFICITY OF THE DSCC METHOD DSCC (%), fluorescence microscopy n = 113 r = DSCC (%), FOSS DSCC method 7

8 SCC REFERENCE 15 SCC ( 1,000 cells/ml), FossomaticFC SCC ( 1,000 cells/ml), FOSS DSCC method n = 640 r = DIFFERENTIAL SCC (DSCC) New business opportunities 16 8

9 IMPROVED BACTERIOLOGICAL EXAMINATION Targeted selection Better interpretation 17 USE OF ANTIBIOTICS AT DRY OFF Improved decision tool 18 9

10 DSCC RESEARCH IN PROGRESS 3-year projects in Microbiological testing Development of guidelines 19 A MESSAGE TO TAKE HOME First high-throughput analyser for DSCC DSCC (%) = PMN + lymphocytes More information on actual inflammatory status New possibilities to further tackle 10

11 KETOSIS SCREENING Experience from around the world 21 FTIR BHB PREDICTION MODEL Indirect calibration developed: Fingerprint area Protein BHB Fat absorbance wavenumber 22 11

12 KETOSIS SCREENING KEYS TO SUCCESS 1) Performance of laboratory analysis 2)Communication of results to dairy farmers 23 QA PROGRAMME IN FRANCE All laboratories offering ketosis screening participate in QA programme: Reference laboratory, wet chemistry method 10 reference samples for BHB ( mmol/l) and 5 samples for acetone ( mmol/l) Frequency: 1/month 24 12

13 IDF GUIDELINE Action Team S03b: New applications of IR spectrometry New guideline to be published in COMMUNICATION OF RESULTS BHB (and Ac) Cow-related data (e.g. DIM) + other parameters, e.g. %fat + cow-site test Ketosis: -yes/no -risk group (e.g. 1-5) -index (e.g. K!) 26 13

14 MILK BHB AND MILK YIELD Test day milk yield (kg) a b a a a a < Milk BHB (mmol/l) 3 kg/day c b b parity 1 parity 2 parity 3 a-c = P <0.05 n = 498, Santschi et al MILK BHB AND MASTITIS Test day SCC (k cells/ml) Santschi et al a a a b b b 184 k cells/ml < Milk BHB (mmol/l) c c c parity 1 parity 2 parity 3 a-c = P <0.05 n = 498,310 14

15 A MESSAGE TO TAKE HOME Simple, practical, rapid and inexpensive tool Keys to success in establishment: QA and communication Evidence of success of ketosis screening in various 15