Cover Page. The handle holds various files of this Leiden University dissertation.

Transcription

1 Cover Page The handle holds various files of this Leiden University dissertation. Author: Dam, G.J. van Title: Circulating gut-associated antigens of Schistosoma mansoni : biological, immunological, and molecular aspects Issue Date:

2 Circulating gut-associated antigens of Schistosoma mansoni: biological, imm unological, and molecular aspects

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4 Circulating gut-associated antigens of Schistosoma mansoni: biological, immunological, and molecular aspects PROEFS CHR IFT ter verkrijging v an de graad van Doctor aan de Rijksuniversit eit te Leiden, op gezag van de Rector M ag nificus Dr. L. Leertouw er, hoogleraar in de faculteit der Godgeleerdheid, volgens besluit van het College van Dekanen te verdedigen op donderdag 9 februari 1995 te klokke uur door Govert Jan van Da m gebore n te Gouda in 1 960

5 iv Pro motieco m missie Promotor Prof. Dr. A. M. Deelder eo-promotor Dr. J.P. Rotmans Referent Prof. Dr. A.E. Butterworth (Cambridge University, Cambridge, UK) Overige leden Prof. Or. B.J. Bogitsh (Vanderbilt University, Nashville, USA) Pro f. Dr. M.R. Daha Prof. Dr. J.P. Kamerling Prof. Dr. W.J.M. Spaan The work described in this t hesis was performed at the Department of Pa rasitology, University of Leiden (Head: Prof. Dr. A.M. Deelder). The financial support for the cost of pri nting given by t he Hubrecht Janssen Fonds, and the Leids Universiteits Fonds is gratefully acknowledged.

6 V our little systems have their day they have their day and cease to be they are but broken lights of Thee but Thou 0 Lord art more than the y Alfred Tennyson aan m1jn vader en moeder aan Betty aan Rieke, Paul en Jaap

7 vi Cover illustrat ion: gut - associated antigens of Schistosoma mansoni, visualized by imm unof luorescence microscopy (McAb 99-1 G3- A) on a section of adult m ale worms fixed in Ro ssm an's fixative CIP - GEGEVENS KO NINKLIJ KE BI BLIO THEEK. DEN HAAG Dam, Govert Jan van Circulating gut - associated antigens of Schistosoma mansoni: biological, immunologica l, and molecular aspects I Govert Jan van Dam. - [S.I. : s.n. l - Ill. Proefschrift Leiden. - M et lit. opg. - Met samenvatting in het Nederlands. ISBN Trefw.: schistosomiasis I biochemie I immunologie.

8 vii Contents Preface ix 1. Schistosoma gut-associated ant igens 1 2. Schistosoma carbohydrates in host-parasite (imm unological) interactions Glycoconjugates in general - short review 2.2. Glycoconjugates of Schistosoma Schistosoma: Analysis of monoc lonal antibodies reactive with t he circulat ing ant igens CAA and CCA Schistosoma m ansoni: analysis of monoclonal antibodies reactive with gut-associated ant igens A pplication of the FITC-anti-FITC-gold system to ultrastructural localizat ion of antigens Detection of lgm ant ibod ies d irected against t he gut-associated circulating cathodic antigen in sera f rom Schistosoma mansoni infected patient s The immunologically react ive 0 -linked polysaccharide chains derived from circulat ing cathodic antigen isolated from the human blood fluke Schistosoma m ansoni have Lewis x as repeating unit The immunologically reactive part of the immunopurified circulating anodic ant igen from Schistosoma mansoni is a threonine-linked polysaccharide consist ing of -6)-[.8-o-GicpA-( 1-3 )]-{J-o-GalpNAc-( 1- repeating unit s Schistosoma mansoni excretory antigen CCA shares carbohydrate epitopes with human granulocytes and evokes host ant ibodies mediating complement-dependent lysis of granulocytes Schistosoma mansoni circulating anodic antigen but not circulating cat hodic antigen interacts with complement component C 1 q 18 9

9 v ii i Contents 11. Schistosoma mansoni: in vitro and in vivo excretion of CAA and CCA by developing schistosomula and ad ult worms 12. General discussion Summary Sa menvatting A ppendices I. List of abbreviations 11. Li st of figures Ill. List of tables IV. Structure of polysaccharides from CAA V. Structure of polysacc harides f rom CCA A cknow ledgement s Curriculum v itae

10 ix Preface

11 x Pretace

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13 xii Preface presented in Chapter 3. To detect other gut-associated antigens as pot ential candidates for imm unodiagnosis, a study was undertaken to analyze McAbs reactive with Schistosoma mansoni gut-associated antigens other than CAA, CCA, o r w ell-studied gut proteases. The outcome of this study, described in Chapter 4, corroborated the important role of CCA. Chapter 5 describes a technical improvement in the detection or ultrastructural localization of antigens using an anti-fitc McAb. As an example the detection of CCA in S. mansoni adult worms is used. In a routinely used serod iagnostic assay {immunof luorescence on sectio ns of adult worms) human lgm antibod ies are measured w hic h are directed against gut-associated antigens (predominantly against CCA). To more spec ifically study the I gm response against CCA and to simplify t his detection an ELISA system was developed w ith the use of immunopurified CC A, as described in Chapter 6. T he immunopuri fic ation of sufficiently large amounts of CAA and CCA allowed for their st ru ct ural analysis. The elucidation of the primary structures of the carbohydrate pa rt s, most relevant immunodiagnostically and im m unologically, is described in Chapters 7 and 8. Because the carbohydrate structure of CCA w as found to resemble a major granulocyte surface antigen, the possible role of ant i CCA antibodies in granulocytotoxicity was investigated in Chapt er 9. In Chapter 10 a possible function for CAA is described, as it was shown to interact w ith the fi rst complement component C1 q, which may res ult in an interference with c ell ular cytotoxicity mechanisms against the parasite. Finally, t o contribute t o the study of the possible physiological role of CAA and CCA, the in vivo and in vitro excretion patterns of CAA and CCA by newly transformed and developing schistosomula as well as by 7-week old adult w orms were investigated and discussed with respect to clearance mechanisms in Chapter 11. This thesis concludes with a short general discussion in Chapter 12.