Blood Alcohol Test No Summary Report

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1 Collaborative Testing Services, Inc FORENSIC TESTING PROGRAM Blood Alcohol Summary Report This test was sent to 84 participants. Each sample pack consisted of four vials of blood with differing amounts of ethanol. Participants were asked to determine the blood alcohol concentrations. Data were returned from 72 participants (86% response rate) and are compiled into the following tables: Page Manfacturer's Information Summary Comments Table 1: Examination Results Table 2: Methods of Analysis & Variability/Accuracy Table 3: Limits of Detection Table 4: Conclusions Table 5: Additional Comments Appendix: Data Sheet This report contains the data received from the participants in this test. Since these participants are located in many countries around the world, and it is their option how the samples are to be used (e.g., training exercise, known or blind proficiency testing, research and development of new techniques, etc.), the results compiled in the Summary Report are not intended to be an overview of the quality of work performed in the profession and cannot be interpreted as such. The Summary Comments are included for the benefit of participants to assist with maintaining or enhancing the quality of their results. These comments are not intended to reflect the general state of the art within the profession. Participant results are reported using a randomly assigned "WebCode". This code maintains participant's anonymity, provides linking of the various report sections, and will change with every report.

2 Manufacturer's Information Each sample pack consisted of four vials of blood with differing amounts of ethanol to produce the target blood alcohol concentrations. A stock solution of ethanol in water was used to spike each item. The stock solution was prepared by combining 10 grams of absolute ethanol with enough distilled water to produce a solution of 100 milliliters. The stock solution was stored in a covered flask, which was opened only when a volume of the solution was needed for production. Sample preparation consisted of partially filling a graduated cylinder with human whole blood (which had been drawn into citric acid preservative bloodbank bags), adding the predetermined amount of ethanol stock solution, and then continuing to fill the graduated cylinder with blood until the desired volume was obtained. The blood mixture was transferred to a beaker and then stirred with a magnetic stirrer for 2 minutes before pipetting the mixture into the prelabeled vials. Each item was prepared separately, and all glassware was cleaned between preparations. The vials were sealed and inverted 8-10 times to mix the potassium fluoride/ sodium oxalate with the blood solution. All vials were placed in a refrigerator immediately after production until the sample packs were prepared. The grand means in the following table are based on the results reported in Table 1B from all responding participants. Item Blood Volume Ethanol Solution Volume Target BAC Grand BAC mL 7.5mL mL 3.5mL mL 10mL mL 5mL ( 2 )

3 Summary Comments This test was designed to allow participants to assess their proficiency in the analysis of blood alcohol concentration in samples of human blood. Each participant was supplied with a sample pack consisting of four vials of blood spiked with differing amounts of ethanol to produce the target blood alcohol concentration (see Manufacturer's Information for production details). The report that follows contains all data as reported by the laboratories. Due to differences in reporting procedures (e.g. mean, lowest result, truncated results) and differences in the number of replicate analyses performed, the standard deviation of each Item is not calculated in this report. The identification of inconsistencies is also not reported for the same reason. ( 3 )

4 Examination Results What is the blood alcohol concentration of the sample in each of the vials? TABLE 1A WebCode Target BAC: 15EJLN Item 1 Item 2 Item 3 Item Reporting Procedures* 1JY6S R9NZ TF7N HVH PCDHU RYET RYANZ ACJT D6A6W ZGDK NAQF LM6QX V6KPT B9DWT ARKE9U BKGQ6V BREMPS CFCJZP D73GDN DUF9GM Single Determination DZHFWQ ECA22K EEFQGA ETYCPM EWZFG ( 4 )

5 Target BAC: Item 1 Item 2 TABLE 1A Item 3 Item Reporting Procedures* F2NLRQ 0.07 F595U F5MWMP F89FQ FGDN FM7NLP 0.06 FWGSU H6UVSH HYX1W J1KZN JC814E JWLL K3PPQG KMCBA MNMZP Truncated MTAKU NCWYJB NHRSGC NKULEH PBFWC PFF5JY PYWBWF Q653XX QEXFXX QPP21A Single Determination QQVG QZHHQB RGLBWB ( 5 )

6 Target BAC: S4CRBX Item 1 Item 2 TABLE 1A Item 3 Item Reporting Procedures* SEW5EZ SXP66W T229UC T51MBA TSBNK TULSXX UJ2LY V1H7Z VBYVU VZ772U WSK1F X5PG Single Determination X8PSX Single Determination YK1CWQ YVKRZS YXMRX ZCFGTZ * "Are the values listed for the blood alchohol concentration: - The mean of several determinations; - The lowest value of several determinations; - Other (specify)?" ( 6 )

7 Target BAC: Examination Results, continued If more than one determination was taken for each item, list the value of each separate determination below. Item 1 TABLE 1B Item Item 3 Item 4 15EJLN JY6S R9NZ HVH RYET RYANZ ACJT D6A6W ZGDK NAQF LM6QX B9DWT ARKE9U ( 7 )

8 Target BAC: Item 1 TABLE 1B Item Item 3 Item 4 BKGQ6V BREMPS CFCJZP D73GDN DZHFWQ ECA22K EEFQGA ETYCPM EWZFG F2NLRQ F595U F5MWMP F89FQ FGDN FM7NLP ( 8 )

9 Target BAC: Item 1 TABLE 1B Item Item 3 Item 4 FWGSU H6UVSH J1KZN JC814E JWLL K3PPQG KMCBA MNMZP MTAKU NCWYJB NHRSGC NKULEH PBFWC PFF5JY ( 9 )

10 Target BAC: Item 1 TABLE 1B Item Item 3 Item 4 PYWBWF Q653XX QEXFXX QQVG QZHHQB RGLBWB S4CRBX SEW5EZ SXP66W T229UC T51MBA TSBNK TULSXX UJ2LY ( 10 )

11 Target BAC: Item 1 TABLE 1B Item Item 3 Item 4 V1H7Z VBYVU VZ772U WSK1F YK1CWQ YVKRZS YXMRX ZCFGTZ Grand : ( 11 )

12 15EJLN Method Method of Analysis & Variability/Accuracy What method of analysis did you use? What is the variability or accuracy (e.g., coefficient of variation) associated with the analysis method used? TABLE 2 Variability/Accuracy Acceptable variation for State crime lab is (±) 5%. 1JY6S1 2R9NZ1 2TF7N1 4HVH21 for standards:±0.004 for sample:±0.01 The control sample result is g/100mL from the known value. The maximum acceptable deviation is ± 0.01g/100ml from the known value. The coefficient of variation of a 0 sample pipetted and GC analyzed 9 times in sequence is 1.8 for BAC 2 and 1.1 for BAC 1. ±5% 5PCDHU ±5% 63RYET 6RYANZ ±1.5% of the mean of the sample runs. 76ACJT CoV <2% 7D6A6W 81ZGDK GC-Liquid Hand Injection Coefficient of variation 3.25% ±5% 89NAQF 8LM6QX 8V6KPT GC- Direct Injection w/internal Control Approx. STD. Deviation Level (g/dl) from Controls from Calibrators Results of replicate analysis must be within 0.005g% of the mean value. 9B9DWT 1 SD = ± ARKE9U 5% BKGQ6V ( 12 )

13 BREMPS Method TABLE 2 Variability/Accuracy Standard Deviation = g/100ml CFCJZP D73GDN DUF9GM Acceptance Criteria: Controls shall be within ±5% of their expected values. The integrator retention time window will be within ±3% of the expected time. Prepared samples (evidence samples) should agree within ±5%. We require the relative percent difference between a sample and duplicate to be ±5%. If greater than 5%, sample and duplicate are re-extracted and re-analyzed. Also, control samples must also be ±5% of expected value. ±5% DZHFWQ ± from mean value ECA22K At 3 standard deviation coefficient of variation =±5%. EEFQGA ±0.003 ETYCPM EWZFG9 F2NLRQ ± 0.008g% is the acceptable range for duplicate analyses. ± 5% of expected value for controls and check standards. ±5% F595U8 F5MWMP F89FQ8 FGDN58 GC- Direct Injection w/internal Control Controls are acceptable within ±5% of the target value. Replicate sample results must agree within 5% of the lowest replicate. Results of replicate analysis must be within 0.005g% of the mean value. Acceptable is ± 5% of the target control, ±0.005g/100mL below 0g/100mL. Actual for each of the two analysis is -1%. ±3% i.e., ±0.003 at 0 FM7NLP FWGSU8 H6UVSH The %RSD of the calibration graph is calculated each day a run is performed. The % RSD for this run is The % RSD must be less than or equal to 10.0%. Unknown sample duplicate differences and all quality control samples must be within 5% of targets. HYX1W5 State regulations require ±10% or 0.01AC whichever is greater. The lab accuracy is within ±5%. ( 13 )

14 J1KZN8 JC814E JWLL34 Method TABLE 2 Variability/Accuracy Coefficiant of variation at the level of 0g/100mL is 1.0%. Coefficient of variation at the level of 0.300g/100mL is 0.8%. ±5% for controls and standards. Duplicate results within 0.008% of each other. Run is accepted when controls are within ±5% of target value. K3PPQG KMCBA5 Maximum allowable deviation between duplicate case samples is 8%. Maximum allowable deviation for QC samples is ±8% from target value. ± MNMZP2 The accuracy of this method is ±5% MTAKU3 NCWYJB NHRSGC Standard Deviation= Relative Standard Deviation= Check standards amd controls must be within 5% of the expected value. Duplicate analyses must agree to within 0.008% of each other. NKULEH ±5% PBFWC3 PFF5JY PYWBWF Q653XX QEXFXX GC- Direct Injection w/internal Control Acceptable accuracy for EtOH analysis is ±5% above 100mg/100mL and ± 5mg/100mL below 100mg/100mL. Precision -.001% BAC Accuracy -.003% BAC Results of replicate analysis must be within 0.005g% of the mean value. The variability or accuracy associated with our analysis method is the mean value of the two acceptable replicate analysis which should be within ±5%. ±5% QPP21A ±5% QQVG92 Relative percent difference of 5% for duplicates. QZHHQB RGLBWB ±5% S4CRBX ± 0.003% by weight ( 14 )

15 SEW5EZ SXP66W T229UC Method TABLE 2 Variability/Accuracy Acceptable is ±5% of target control (±0.0005g/100mL below 0g/100mL) ACTUAL is -1% for the two analyses. ±5% for known standards (whole blood controls and aqueous verification standards*) and ±5 duplicate difference between two determinations. *The low verification standard is ±0.004 or ±5% whichever is greater. T51MBA ±0.01 g ethanol per 100 ml blood. TSBNK8 TULSXX GC (Not Headspace) The allowable variability for samples with a concentration less than is ±0.01 of the expected value and between samples. For samples with a concentration greater than, the % of value then must be within 10% of the expected value and between sample results. ± 5% UJ2LY g/100ml V1H7Z9 ±0.01% w/v VBYVU8 CoV =3.07 VZ772U WSK1F7 GC-Whole Blood Injection ±0.005 g/100ml blood as measured at 0g/100ml. CoV=3.3% ±5% X5PG23 X8PSX3 ±5% YK1CWQ YVKRZS YXMRX4 GC-Liquid Injection ±5%, (Duplicate determinations must be within ±5% of each other. Whole blood controls and calibrators must be within ± 5% of each other.) There is a 99.8% confidence that the true blood alcohol level is within ±6.4% of the mean of the duplicate results. ZCFGTZ Check standards and controls must be with 5% of the expected value. Duplicate analyses must agree to within 0.008g% of each other. ( 15 )

16 15EJLN 1JY6S1 2R9NZ1 2TF7N1 4HVH21 5PCDHU 63RYET 6RYANZ 76ACJT 7D6A6W 81ZGDK 89NAQF 8LM6QX 8V6KPT 9B9DWT ARKE9U BKGQ6V BREMPS Limits of Detection What are the limits of detection, or lower and upper limits of quantitation? TABLE 3 Limits of Detection Lower detection limit is 8000 area counts under the curve, or less than 0.01% w/v ethyl alcohol. Limit of detection 0.008% Ethanol Limit of Quantitation: 0.01% Ethanol 0.02 to 0.40 Lower g/100ml Upper 0.400g/100mL LOD wt/vol% LOQ wt/vol% (lower limit) LOD=0.001g/100mL. LOQ=0.01g/100mL lower Limit of detection established at 0.005g% Lower limit of quantitation established at 0.01g% Upper limit of quantitation established at 0.50g% Limit of Detection=0.0004g/100mL Lower limit of Quantitation=0.0015g/100mL Upper limit of Quantitaion=0.30g/100mL LOD=0.001g/dL LOQ= g/dl 0.02g% to 0.40g% LOQ=0.001% ULOQ=0.4% Limit of detection=0.004 Limits of Quantitation (ethanol) 0.01 Values greater than 0.590g/100mL will be diluted and reanalyzed. Lower g/100ml Upper g/100ml We use a calibration curve from g/100ml. Upper: 0.50g/100mL Lower: 0.01g/100mL ( 16 )

17 CFCJZP D73GDN DUF9GM DZHFWQ ECA22K EEFQGA ETYCPM EWZFG9 F2NLRQ F595U8 F5MWMP F89FQ8 FGDN58 FM7NLP FWGSU8 H6UVSH HYX1W5 J1KZN8 JC814E JWLL34 TABLE 3 Limits of Detection Lower limit of quantitation=.01 (any result between.0001 and.0099 g/100cc is reported as trace amounts of ethyl alcohol). Upper limit of quantitation=undetermined 0.00% and 0.237%, 3 point calibration curve. 0.01g% to 0.50g% 0g%-0.300g% LOD=0.005 g/100ml LOQ=0.010 g/100ml ULOQ=1.0 g/100ml LLQ=0.01% by wt ULQ = 0.05% by wt 0.01g/100mL-lower limit 0.50g/100mL-Upper limit to g/100ml g/100ml Lower limit of detection defined by procedure to be 0.005g/dL. calibration point 0.01g/dL. Lower limit for reported quantitation 0.02g/dL. Upper limit for reported quantitation 0.50g/dL. 0.02g% to 0.40g% LOD=0.500g/100mL LLOQ=0.02g/100mL (minimum reportable concentration). ULOQ=0.500g/100mL Lower limit g/100ml Upper limit g/100ml LOQ: 0.01%(w/v) Lower %(w/v) Upper Samples containing less than 0.01% w/v are reported as an insignificant amount. The highest calibrator value is 0.30% w/v. However, the method has been tested and shown to be linear to 1.0% w/v. Lower: 0.01g ETOH/100mL Upper: 0.40 g ETOH/100mL AC to AC LOD, LLOQ and ULOQ have not been accurately determined yet but LOD is less than 5mg/dL (<0.005g/100mL) and LLOQ is less than 10mg/dL (<0.010g/100mL) and VLOQ is greater than 400mg/dL (>0.400g/100mL) %-0.400% 0.01g/100mL g/100mL. ( 17 )

18 K3PPQG KMCBA5 MNMZP2 MTAKU3 NCWYJB NHRSGC NKULEH PBFWC3 PFF5JY PYWBWF Q653XX QEXFXX QPP21A QQVG92 QZHHQB RGLBWB S4CRBX SEW5EZ SXP66W T229UC lower limit of quantitation is upper limit of quantitation is LLQ = 0.01% by wt. ULQ % by wt. The lower limit of quanitation is 0.01%. The upper limit of quantitation is 0.40%. LOD= g% 0.015% Lower Limit of quantitation 5mg/100mL. Upper Limit of quantitation 1000mg/100mL. lower limit <0.005% BAC upper limit 0.50% BAC 0.02g% to 0.40g% TABLE 3 Limits of Detection Our blood alcohol limits of quantitation are 0.01 g/100ml for lower limit, and 0.50 g/100ml for upper limit. LOL= 0.01 LOL=lower limit of detection Lower limit of Quant = Upper limit of Quant = g% to 0.50g%.020 lower limit of Quantitation.500 upper limit of Quantitation Lower gram/100 milliliters Upper 0.50 gram/100 milliliters lower level of detection is grams per 100 milliliters upper level of detection is grams per 100 milliliters Lower limit of quant = 0.01% by weight Upper limit of quant = 0.50% by weight LLOD=0.005 g/100ml LLOQ=0.02 minimum concentration reported ULOQ=0.500 The method is validated from to a 1.0%. limit of detection is below 0.010% w/v. Limit of quantitation is 0.015% w/v. ( 18 )

19 T51MBA TSBNK8 TULSXX UJ2LY8 V1H7Z9 VBYVU8 VZ772U WSK1F7 X5PG23 X8PSX3 YK1CWQ YVKRZS YXMRX4 ZCFGTZ TABLE 3 Limits of Detection lower limit of quantitation 0.02 g ethanol per 100 ml blood. upper limit of quantitation g ethanol per 100 ml blood. The lower limit of detection falls below The upper limit of detection has not been determined. Currently, the instrument normally checked with a standard 0.40 as the upper limit that fits along the linearity. LOQ g/100ml LOQ g/100ml g/100ml Lower Limit of Quantitation-.01g/100mL. Upper Limit of Quantitation-.40g/100mL g/100mL EtOH lower upper both g/100ml blood. Linear range of g/100mL to g%-0.50g% From 0 (zero) to 0.6. The lower limit of the quantitation is set 0.010g/100mL..010% -.300% ( 19 )

20 Conclusions What would be the wording of the Conclusions in your report? TABLE 4 WebCode 15EJLN 1JY6S1 2R9NZ1 2TF7N1 4HVH21 5PCDHU 63RYET 6RYANZ 76ACJT 7D6A6W 81ZGDK 89NAQF 8LM6QX 8V6KPT 9B9DWT ARKE9U BKGQ6V Conclusions Item X analysis of the specimen showed it to contain 0.XXX% w/v ethyl alcohol. 0.XXgram of ethyl alcohol per 100 milliliters of whole blood. Ethanol was identified in each of the blood samples in concentration reported in (Table 1A). [No Conclusions Reported] Item 1: Analysis of the blood showed it to contain 0.149% w/v ethyl alcohol. Item 2: Analysis of the blood showed it to contain 0.068% w/v ethyl alcohol. Item 3: Analysis of the blood showed it to contain 0.199% w/v ethyl alcohol. Item 4: Analysis of the blood showed it to contain 0.097% w/v ethyl alcohol. This analysis was conducted by Gas Chromatography in accordance with State Department of Health Services Regulation, which includes a Quality Assurance Program to ensure the validity of test results. The blood from specimen 1 has an ethanol content of 0.148%. Analysis was preformed using gas chromatography. The blood from Spec 1 has an ethyl alcohol content of 0.000%. Analysis was performed using gas chromatography. Positive Ethyl Alcohol wt/vol% Item 1: The sample contained grams of alcohol per 100mL. Item 2: The sample contained 0.07 grams of alcohol per 100mL. Item 3: The sample contained 0.19 grams of alcohol per 100mL. Item 4: The sample contained grams of alcohol per 100mL. Item 1: The blood contains 0.13 of one percentum of ethyl alcohol by weight. If a swab was submitted: Ethyl alcohol (was/was not) detected on the submitted swab. On analysis the blood was found to contain not less than X milligrams of alcohol per 100 milliliters of blood. X = the reportable alcohol concentration is 5% of reading less than the mean value of the four acceptable results. [No Conclusions Reported] The blood in Item 1 was determined to contain 0.14g% ethyl alcohol. The blood in Item 2 was determined to contain 0.06g% ethyl alcohol. The blood in Item 3 was determined to contain 0.19g% ethyl alcohol. The blood in Item 4 was determined to contain g% ethyl alcohol % w/v Ethanol found in blood. Using Item 1 results as an example grams alcohol per 100 milliliters Blood. Specimen 1 is positive for Ethanol wt/vol%. Analysis of Item X indicated that the item contained 0.XXX grams of ethanol per 100 milliliters of blood. ( 20 )

21 TABLE 4 WebCode Conclusions BREMPS CFCJZP D73GDN DUF9GM DZHFWQ ECA22K EEFQGA ETYCPM EWZFG9 F2NLRQ F595U8 F5MWMP F89FQ8 FGDN58 Item 1: The blood contains grams of ethyl alcohol per 100 milliliters. Item 2: The blood contains grams of ethyl alcohol per 100 milliliters. Item 3: The blood contains grams of ethyl alcohol per 100 milliliters. Item 4: The blood contains grams of ethyl alcohol per 100 milliliters. Examination of this whole blood specimen reveals an alcohol level of X percent by weight (X grams of ethyl alcohol per 100cc blood). [No Conclusions Reported] Gram % of Ethyl alcohol. Tube 1: Blood alcohol result: 0.16g% Tube 2: Blood alcohol result: 0.07g% Tube 3: Blood alcohol result: 0.22g% Tube 4: Blood alcohol result: g% 001 Blood Alcohol Result: grams percent 002 Blood Alcohol Result: grams percent 003 Blood Alcohol Result: grams percent 004 Blood Alcohol Result: grams percent Ethyl alcohol by weight:. Item 1 contained 0.13 grams of alcohol per 100 milliliters of blood. Item 2 contained 0.05 grams of alcohol per 100 milliliters of blood. Item 3 contained 0.18 grams of alcohol per 100 milliliters of blood. Item 4 contained 0.08 grams of alcohol per 100 milliliters of blood. The blood from Specimen 1 has an ethyl alcohol content of 2%. The blood from Specimen 2 has an ethyl alcohol content of 0.070%. The blood from Specimen 3 has an ethyl alcohol content of 3%. The blood from Specimen 4 has an ethyl alcohol content of 0%. Item 1 Ethyl Alcohol grams per 100 milliliters of blood. Item 2 Ethyl Alcohol 0.07 grams per 100 milliliters of blood. Item 3 Ethyl Alcohol grams per 100 milliliters of blood. Item 4 Ethyl Alcohol grams per 100 milliliters of blood. Item specimen analyze result method, Item 1 blood ethanol 0.147g/100mL HS/GC. Any result less than 0.02 g/100ml would be reported as negative for ethanol. The blood in Item 1 was determined to contain 0.14g% ethyl alcohol. The blood in Item 2 was determined to contain 0.06g% ethyl alcohol. The blood in Item 3 was determined to contain 0.19g% ethyl alcohol. The blood in Item 4 was determined to contain g% ethyl alcohol. For results </=0.02 g/100ml, the numerical result will be reported. Any result <0.02g/100mL will be reported as "Negative". Item g/100ml Ethanol. Item g/100ml Ethanol. Item g/100ml Ethanol. Item g/100ml Ethanol. ( 21 )

22 TABLE 4 WebCode Conclusions FM7NLP FWGSU8 H6UVSH HYX1W5 J1KZN8 JC814E JWLL34 K3PPQG KMCBA5 MNMZP2 MTAKU3 Analysis of vial (Item 1) indicated that the blood contained % blood alcohol or 1.5mg of ethyl alcohol per ml of blood. Analysis of vial (Item 2) indicated that the blood contained 0.06% blood alcohol or 0.6mg of ethyl alcohol per ml of blood. Analysis of vial (Item 3) indicated that the blood contained % blood alcohol or 2.0mg of ethyl alcohol per ml of blood. Analysis of vial (Item 4) indicated that the blood contained % blood alcohol or 1.0mg of ethyl alcohol per ml of blood. Analysis of the blood showed it to contain 0.146% w/v ethyl alcohol. (Same for each item). Item 1 The sample contained g of alcohol per 100mL. Item 2 The sample contained 0.07g of alcohol per 100mL. Item 3 The sample contained 0.21g of alcohol per 100mL. Item 4 The sample contained g of alcohol per 100mL. Analysis of the submitted specimen showed the presence of XXX grams of alcohol/100ml whole blood or, XXXg/100mL. Example: The blood from the tube marked "Item 1" contained 150 milligrams of alcohol per 100 millitres. No such congealing or deterioration of the blood was found as would prevent proper analysis. The blood in Item 1 has an ethyl alcohol content of 2% The blood in Item 2 has an ethyl alcohol content of 0.071% The blood in Item 3 has an ethyl alcohol content of 3% The blood in Item 4 has an ethyl alcohol content of 1% Analysis was performed using gas chromatography. Analysis of the blood showed it to contain 0.XXX gram of ethyl alcohol per 100 milliliters of blood. Item 1- Headspace gas chromatographic analysis of the blood specimen received resulted in the identification of ethyl alcohol in the amount of fourteen-hundredths of one gram percent (0.14%) by weight in whole blood. Item 2- Headspace gas chromatographic analysis of the blood specimen received resulted in the identification of ethyl alcohol in the amount of six-hundredths of one gram percent (0.06%) by weight in whole blood. Item 3- Headspace gas chromatographic analysis of the blood specimen received resulted in the identification of ethyl alcohol in the amount of twenty-hundredths of one gram percent (%) by weight in whole blood. Item 4- Headspace gas chromatographic analysis of the blood specimen received resulted in the identification of ethyl alcohol in the amount of nine-hundredths of one gram percent (0.09%) by weight in whole blood. Item 1 - Ethyl Alcohol % by weight: 0.14 Item 2 - Ethyl Alcohol % by weight: 0.06 Item 3 - Ethyl Alcohol % by weight: 0.19 Item 4 - Ethyl Alcohol % by weight: 0.09 Ethanol Result: #.##% Item 1. The blood sample submitted labeled, " Item 1" has an Ethyl Alcohol Content of 0.147%. Blood Alcohol Analysis was performed by gas chromatography by ambient headspace. ( 22 )

23 TABLE 4 WebCode NCWYJB NHRSGC NKULEH PBFWC3 PFF5JY PYWBWF Q653XX QEXFXX QPP21A QQVG92 QZHHQB RGLBWB S4CRBX SEW5EZ SXP66W T229UC Conclusions The blood from Item 1 has an ethyl alcohol content of X%. Analysis was performed using Gas Chromatography. [No Conclusions Reported] 1. Ethyl Alchol Positive wt/vol% 2. Ethyl Alchol Positive wt/vol% 3. Ethyl Alchol Positive wt/vol% 4. Ethyl Alchol Positive wt/vol% No conclusions would be appended in a typical report with findings of blood alcohol as detected in this proficiency test. Final Blood Alcohol result of 0.14% (wt/vol) is an average of 2 determinations. (Sample). The blood in Item 1 was determined to contain 0.14g% ethyl alcohol. The blood in Item 2 was determined to contain 0.06g% ethyl alcohol. The blood in Item 3 was determined to contain 0.19g% ethyl alcohol. The blood in Item 4 was determined to contain 0.09g% ethyl alcohol. [No conclusions reported] Alcohol result of Item 1 = 0.14 grams % Alcohol result of Item 2 = 0.06 grams % Alcohol result of Item 3 = 0.19 grams % Alcohol result of Item 4 = 0.09 grams % grams % = g/100ml g% of Ethyl Alcohol. X.XXX grams/deciliter Ethyl Alcohol The sample contained grams of alcohol per 100 milliliters of blood. The sample contained 0.06 grams of alcohol per 100 milliliters of blood. The sample contained grams of alcohol per 100 milliliters of blood. The sample contained 0.09 grams of alcohol per 100 milliliters of blood. Item 1 The sample contained grams of alcohol per 100 milliliters. Item 2 The sample contained 0.07 grams of alcohol per 100 milliliters. Item 3 The sample contained grams of alcohol per 100 milliliters. Item 4 The sample contained 0.09 grams of alcohol per 100 milliliters. Ethyl alcohol percent by weight :. Analytical results for any result >/=0.02g/100mL. "Negative" for any result < 0.02g/100mL. Analysis of the specimen showed it to contain X% w/v ethyl alcohol. Ethyl alcohol-positive - "amount" wt/vol% ( 23 )

24 TABLE 4 WebCode T51MBA TSBNK8 TULSXX UJ2LY8 V1H7Z9 VBYVU8 VZ772U WSK1F7 X5PG23 X8PSX3 YK1CWQ YVKRZS YXMRX4 ZCFGTZ Conclusions Item grams ethanol per 100 ml of blood. Item grams ethanol per 100 ml of blood. Item grams ethanol per 100 ml of blood. Item 4 - grams ethanol per 100 ml of blood. The sample contains X grams of alcohol per 100mL. The blood was analyzed and found to contain 0 grams of alcohol per 100 millilitres of blood (0 per centium). (Item 1 Example) Item 1-.14g/100mL Item 2-.07g/100mL Item 3-.20g/100mL Item 4-.10g/100mL. Reported to two decimal places as "Reported Amount": % w/v in blood 0.07% w/v in blood % w/v in blood % w/v in blood Ethyl Alcohol-(value) of one percent, by weight State required formal giving alcohol content (in g/100 ml blood) and percent number. The alcohol level would be reported as the number in g% Item 1 - Ethyl Alcohol (gram %). Item Ethyl Alcohol (gram %). Item Ethyl Alcohol (gram %). Item 4 - Ethyl Alcohol (gram %). g% of ethyl alcohol. Item 1- Analysis of the specimen showed it to contain 0.146% w/v ethyl alcohol. Item 2- Analysis of the specimen showed it to contain 0.067% w/v ethyl alcohol. Item 3- Analysis of the specimen showed it to contain 0.198% w/v ethyl alcohol. Item 4- Analysis of the specimen showed it to contain 0.098% w/v ethyl alcohol. [No conclusions reported] Item 1: The sample contained grams of alcohol per 100mL. Item 2: The sample contained 0.06 grams of alcohol per 100mL. Item 3: The sample contained 0.21 grams of alcohol per 100mL. Item 4: The sample contained grams of alcohol per 100mL. The blood from Specimen 1 has an ethyl alcohol content of 1%. Analysis was performed using gas chromatography. (Same wording for additional specimens). ( 24 )

25 Additional Comments TABLE 5 WebCode 7D6A6W 89NAQF J1KZN8 K3PPQG Q653XX TSBNK8 YVKRZS Additional Comments The instrument is calibrated every time data is reported out. A blank sample is checked between each case. A Bio-Rad (level 2) control is run each day the instrument is calibrated. Reports issued by our laboratory on forensic case evidence are in the form (.XX g/100ml), where 0.XX is the lowest value of several determinations (to three decimal places, truncated to two decimal places). In the case of Item 3, this would have resulted in a report of 0.19 g/100ml, rather than g/100ml. If a blood sample is analyzed under road transport law, for all results of 0.036g/100mL and greater, a deduction of 0.006g/100mL is made prior to reporting. This deduction is made to allow for analytical uncertainty and to give a very high degree of confidence that the reported result is not greater than the true alcohol concentration. We only analyze whole blood samples (case samples) for the presence of ethanol from living subjects. Hence, we do not screen our case samples for the presence of n-propanol, which we use as the internal standards prior to the BAC determination. With the dual column procedure, small amounts of Acetaldehyde were detected in all four (4) proficiency test samples. Results are reported to two decimal places. Third decimal place is truncated. Reporting statement is in conjunction with State Laws. Reported results are the lowest result truncated to the hundredth decimal place. Analytical Method-1:10 dilution of blood with n-propanol solution, protein precipitation with tungistic acid. Internal Standard- N-propanol. ( 25 )

26 Appendix: Data Sheet Collaborative Testing Services ~ Forensic Testing Program : Blood Alcohol DATA MUST BE RECEIVED BY February 9, 2004 TO BE INCLUDED IN THE REPORT Participant Code: U2483A WebCode: TULSXX Test Description: Investigators have submitted four vials of blood from separate cases to be examined; each was taken from a person suspected of being intoxicated. Using your laboratory's procedures, analyze each vial and report the blood alcohol concentration. Items Submitted (Sample Pack BA): Item 1-4: Vials of Blood ASCLD/LAB Release If your lab has been accredited by ASCLD/LAB and you are submitting this data as part of their external proficiency test requirements, have the Lab Director or Quality Assurance Manager complete the following. The information below must be completed in its entirety for the results to be submitted to ASCLD/LAB. ASCLD/LAB Certificate No. Signature Date Laboratory Name Location (City/State) 1.) What is the blood alcohol concentration of the sample in each of the vials? Item 1: g/100 ml Item 3: g/100 ml Item 2: g/100 ml Item 4: g/100 ml 2a.) Are the values listed above: The mean of several determinations? The lowest value of several determinations? Other? (Specify): ( 26 )

27 Page 2 of 3 2b.) If more that one determination was taken for each item, you may list the value of each separate determination below. (Include results from Question 1 if applicable). Item 1 Item 2 Item 3 Item 4 3.) What method of analysis did you use? Gas Chromotography: Headspace Other (specify): 4.) What is the variability or accuracy (e.g., coefficient of variation) associated with the analysis method used? 5.) What are the limits of detection, or lower and upper limits of quantitation? ( 27 )

28 Page 3 of 3 6.) What would be the wording of the Conclusions in your report? 7.) Additional Comments 8a.) Date Samples Received: 8b.) Date(s) Samples Analyzed: Return Instructions Data Sheets can be mailed or faxed (please include a cover sheet) and must be received by Monday, February 09, 2004 to be included in the report. MAIL: Collaborative Testing Services, Inc. Forensic Testing Program FAX: or Toll-Free (U.S. only): FAX-2CTS P.O. Box TEL: (8 am - 4:30 pm EST) Sterling, VA USA forensics@cts-interlab.com ( 28 )