Determination of Ethanol in Breath and Estimation of Blood Alcohol Concentration with Alcolmeter S-D2
|
|
- Anthony Austin
- 6 years ago
- Views:
Transcription
1 Determination of Ethanol in Breath and Estimation of Blood Alcohol Concentration with Alcolmeter S-D2 A.W. Jones and KÄ. Jönsson Departments of Alcohol Toxicology and Internal Medicine, University Hospital, Linköping, Sweden. 1. Introduction The technology of breath-alcohol analysis can be subdivided into two broad categories; devices for pre-arrest screening of drunk drivers and more sophisticated instruments for quantitative evidential purposes (1,2). The Lion Alcolmeter S-D2 is a multipurpose hand-held device designed originally for use as a roadside screening test. However, its easy-to-read digital display and highly reproducible breath sampling system, has created many applications for this instrument in alcohol research, industrial chemistry, and clinical and emergency medicine. Although the Lion Alcolmeter S-D2 has been available for many years, hardly any studies have dealt with its precision and accuracy. When breath-alcohol analyzers are used for legal purposes the results are mostly reported as the concentration in the breath sample analyzed (BrAC) expressed in units of mg/l, jig/loo ml, or g/ However, when breath-alcohol analyzers are used in research and clinical medicine, the BrAC is almost always translated into the presumed concentration in a specimen of blood (BAC) having units of g/1, mg/dl, or g% w/v. To make the conversion from BrAC into BAC, one assumes a constant blood:breath ratio of alcohol (usually 2100:1 or 2300:1), which is used to calibrate the instrument (3). The precision and accuracy of Lion Alcolmeter S-D2 was evaluated in experiments in-vitro with known strength air-alcohol standards and also invivo with volunteer subjects who ingested moderate amounts of alcohol. 2. Material and methods 2.1 Breath-alcohol instruments Four examples of the Lion Alcolmeter S-D2 breath-alcohol analyzer were provided by the manufacture (Lion Laboratories PLC, Barry, South Alcohol, Drugs and Traffic Safety - T92 Ed. by Utzelmann / Berghaus I Kroj Verlag TÜV Rheinland GmbH, Köln
2 Glamorgan, Wales, UK). The Alcolmeter instruments were fitted with digital scales marked in mg/1 breath and readings could be made to two decimal places. After an initial control of the calibration, no further adjustments were made throughout the experiments. Another Alcolmeter S-D2 instrument was available and this had a digital scale marked in terms of mg/dl presumed blood-alcohol concentration based on a 2300:1 blood:breath ratio. 2.2 Experiments in vitro Air-alcohol mixtures were generated using a wet-bath simulator device manufactured by Guth Laboratories, Harrisburg, USA. The simulator operates at 34 C and the air/water partition coefficient of ethanol at this temperature was taken from the literature, being x 10'3 (4). The concentration of ethanol in the equilibrated air-vapor emerging from the simulator was calculated as the product of the concentration of ethanol and the air/water partition coefficient. The simulator was charged with aqueous ethanol standards prepared from absolute ethanol by first making a 10% w/v stock solution and then volumetric dilution to give working standards at concentrations of 50, 100, 175, 380 and 640 mg/dl. The exact concentrations prepared were checked by headspace gas chromatography and were within ± 2% of the target values and often much better than this. The Lion Alcolmeter S-D2 instruments were fitted with mouth-pieces normally supplied. These tubes were joined to the outlet of the wet-bath simulator using a 2 cm long plastic tubing. The operator blew through the simulator to produce an equilibrated breath-sample at 34 C. When the airvapor standard had passed through the simulator for about 5 seconds, the READ button of the Lion Alcolmeter S-D2 was pressed capturing a sample for analysis. The 4 Alcolmeter S-D2 instruments were positioned side-by-side and exposed 10 times to each of the air-alcohol vapor mixtures. The same simulator charge was used for all 10 tests at each concentration of ethanol. The time interval between making the replicate tests varied from < 5 min to 15 min and was sometimes longer depending on practical circumstances. 23 Experiments in-vivo Ten male volunteers took part in these experiments. They drank a dose of ethanol equivalent to 0.80 g ethanol per kg body weight in 30 minutes. Ethanol was ingested in the morning, either after an overnight fast or after the volunteers had eaten a standard breakfast. An indwelling catheter was implanted in a cubital vein for taking blood samples at exactly 10, 20, 30, 424
3 45, 60, 90, 120, 150, 180, 240, 300, 360, and 420 min after the start of drinking. Blood was drawn into 5 ml Vacutainer tubes (Becton Dickinson, USA) containing sodium fluoride (20 mg) and 75 units heparin. The catheter tubing was flushed with heparin/saline between taking successive samples. The concentration of ethanol in blood was determined in duplicate by headspace gas chromatography as described in detail elsewhere (5). The standard deviation (SD) of this method was 0.6 mg/dl at a mean BAC of 80 mg/dl. 3. Results 3.1 Precision and accuracy of analysis in-vitro Table 1 summarizes the precision and accuracy of the Alcolmeter S-D2 when used to analyze air-alcohol vapor standards. The coefficients of variation (CV), as one index of precision, were between 2% and 2.5% at ethanol concentration up to 0.40 mg/1 and the mean response was within ± 0.02 mg/1 (± 1%) of the target values. When exposed to higher airvapor concentrations of alcohol, the CV was between 2% and 5% and accuracy was within ± 6.6% of the target values. The consecutive tests at 5-10 min intervals tended to decrease as the number of tests increased at concentrations of ethanol at 0.70 mg/1 and higher. The components of variance between and within instruments depended in part on the concentration of alcohol in the air-vapor samples analyzed. At 0.20 mg/1, the SD between instruments was mg/1 and within instruments the SD was mg/1. The pure analytical SD was mg/1. Table 1. Precision and accuracy of Alcolmeter S-D2 when used to analyze air-alcohol vapor standards produced from a breath simulator device. BrAC Target Value mg/1 Mean Response mg/1 (N = 10) SD mg/1 CV% Bias % 0.20 (46) (92) (161) (343) (575) Approximate blood-alcohol concentration in mg/dl based on 2300:1 ratio. 425
4 3.2 Experiments in-vivo Figure 1 gives two examples of blood and breath ethanol profiles for a relatively fast and slow absorber of alcohol. T im e, min T im o, min Fig. 1. Concentration-time profiles of ethanol in venous blood analyzed by gas chromatography and values estimated using Lion Alcolmeter S-D2. The mean difference between duplicate determinations with Alcolmeter S- D2 (N = 109) was ± 0.26 mg/dl (± SD) and this was not significantly different from zero. The SD of the differences was 2.81 mg/dl and the 95% range of individual differences was from -5.5 to +5.7 mg/dl. The precision of a single determination is given by 2.81/21being 1.98 mg/dl expressed in terms of the coexisting BAC. The mean difference between venous BAC and Alcolmeter S-D2 response (N = 109) was ± 0.81 mg/dl (±SD) 426
5 and this was not significantly different from zero. The SD of the differences and the 95% limits of agreement were 8.45 mg/dl and -17 to + 16 mg/dl respectively. Figure 2 shows a blood/breath scatter plot of venous blood alcohol (x-variate) and breath alcohol (y-variate) analyzed with Lion Alcolmeter S-D2. The high correlation (r = 0.94), near zero intercept and slope close to unity confirms the reliability of this device and the lack of bias when used to estimate blood alcohol concentration O 05 E 80 OJ Q1 (/) 60 L_ <D E 40 o < Venous Blood Alcohol, m g/dl Fig. 2. Scatter plot of venous blood alcohol and estimated BAC according to tests with Lion Alcolmeter S-D2. Note the near zero intercept and slope close to unity. 3 3 Variability between instruments Figure 3 compares the concentration-time profiles of ethanol in 2 subjects tested in rapid succession with each of 4 Alcolmeter S-D2 instruments. The SD of breath-alcohol measurements between instruments was 0.01 mg/dl which corresponds to 2.3 mg/dl BAC equivalent. 4. Discussion The uncertainty of any breath-alcohol test depends on analytical and physiological sources of variation. Physiological variations such as the way the subject exhales - pre-breathing maneuver - dominates the total 427
6 variability. Differences between instruments might depend on the care taken in calibration procedure. The relative contribution of analytical and biological sources of variation can be evaluated using analysis of variance after making replicate measurements in each subject with several different instruments. T im e, h T im #, h Fig. 3. Concentration-time profiles of ethanol in breath for 2 subjects tested with 4 different Alcolmeter S-D2 instruments. The in-vitro experiments showed that at high concentrations of alcohol in the breath (> 0.40 mg/1 or 92 mg/dl BAC) the Alcolmeter response tended to decrease slightly as the number of tests made at short intervals (5 min) increased. This observation has been reported elsewhere and is caused by a "fatigue effect" of the electrochemical cell (6). Presumably, the products of ethanol oxidation (acetaldehyde) requires time to diffuse away from the electrode surface interfering with the oxidation of ethanol in each new test. Experiments with human subjects showed that the Alcolmeter response 428
7 was mostly higher than venous BAC during the absorption-distribution phase of ethanol kinetics. In the post-absorptive phase the Alcolmeter response was almost always less than the venous BAC (4). This observation is not new and reflects the uneven distribution of alcohol in the vascular system and arterio-venous difference existing after subjects ingested a bolus dose of ethanol on an empty stomach. Breath-alcohol follows more closely the arterial blood concentration and this exceeds the venous BAC until equilibrium distribution of alcohol in all body fluids and tissue is complete. Moreover, the magnitude and direction of these BAC-BrAC differences depends also on the value of the blood/breath calibration factor used. In conclusion, the precision and accuracy of Lion Alcolmeter S-D2 was exceptionally good for a hand-held instrument with manual sampling procedure. The training and experience of the person operating the unit plays an important role in the precision of breath sampling. As expected, accuracy and precision in-vitro was better than in tests with human subjects. The stability of the calibration was remarkably good over long periods and no adjustments were deemed necessary. The calibration of the instruments should be checked periodically but making haphazard changes is not recommended. 5. References 1. Dubowski, KM. The technology of breath-alcohol analysis. US Department of Health and Human Services, DHHS publication No (ADM) , 1992, pp Jones, AW. The measurement of alcohol in blood and breath for legal purposes. In Human Metabolism of Alcohol, Vol. 1, edited by K E. Crow and R.D. Batt, CRC Press, 1989, pp Jones, AW. Blood and breath alcohol concentrations, Brit. Med. J. 305:955: Jones, AW. Variability of blood:breath alcohol ratio in-vivo. J. Stud. Ale. 1978;39: Jones AW, Schuberth, J. Computer-aided headspace gas chromatography applied to blood-alcohol analysis: Importance of on-line process control. J Forens Sei 1989;34: Jones AW. Evaluation of breath alcohol instruments: In-vitro experiments with Alcolmeter pocket model. Forens Sei Int 1978;12:
Recent Advances in the Analysis of Ethanol in Saliva: Evaluation of the QED Device
Recent Advances in the Analysis of Ethanol in Saliva: Evaluation of the QED Device A.W. Jones and k A. Jünsson Departments of Alcohol Toxicology and Internal Medicine, University Hospital, 581 85 Linköping,
More informationMAGNITUDE OF SAMPLING AND ANALYTICAL VARIATIONS IN BLOOD AND BREATH ALCOHOL MEASUREMENTS
MAGNITUDE OF SAMPLING AND ANALYTICAL VARIATIONS IN BLOOD AND BREATH ALCOHOL MEASUREMENTS A. W. Jones, K. Jensson and P.M. Williams Department of alcohol Toxicology,j National Laboratory of Forensic Chemistry
More informationETHANOL + NAD > ACETALDEHYDE + NADH + H* ACETALDEHYDE + TRIS > COMPLEX NADH + ELECTRON SINK (ES) > NAD + H2 - ES
A COMPARISON BETWEEN ALCOHOL IN SALIVA MEASURED BY AN ENZYMATIC PROCEDURE AND EVIDENTIAL BLOOD AND BREATH ALCOHOL VALUES Ronald C. Denney, University of Greenwich, London. John Matthews, Neil Spencer &
More informationINTER- AND INTRA-INDIVIDUAL VARIATIONS OF BLOOD/BREATH AND SALIVA/BREATH RATIOS OF ALCOHOL
INTER- AND INTRA-INDIVIDUAL VARIATIONS OF BLOOD/BREATH AND SALIVA/BREATH RATIOS OF ALCOHOL Wojciech GUBA A 1, Dariusz ZUBA 2 1 Institute of Forensic Research, Cracow, Poland 2 Faculty of Chemistry, Jagiellonian
More informationBetween-subject and within-subject variations in the
Br J clin Pharmac 1994; 37: 427-431 Between-subject and within-subject variations in the pharmacokinetics of ethanol A. W. JONES' & K. A. JONSSON2 'Departments of Alcohol Toxicology and 2Internal Medicine,
More informationLOW ALCOHOL BEERS AND LAGERS AND BLOOD ALCOHOL LEVELS. Ronald C. Denney. Oniversity of Greenwich, London, SE1B 6PF, England
LOW ALCOHOL BEERS AND LAGERS AND BLOOD ALCOHOL LEVELS Ronald C. Denney Oniversity of Greenwich, London, SE1B 6PF, England 1. InLroduction Much confusion has arisen in recent years in the United Kingdom
More informationModern Developments in Measuring and Screening
Modern Developments in Measuring and Screening W. Bonte and G. Machata The purpose of this paper is to give an introduction into the scientific sessions which deal with measuring and screening. Without
More informationTraceability of breath alcohol concentrations in Romania
BREATH TESTING Traceability of breath alcohol concentrations in Romania MIRELA ADELAIDA ANGHEL Scientific Researcher III, Physico-Chemical Laboratory, National Institute of Metrology, Bucharest, Romania
More informationNATIONAL SAFETY COUNCIL COMMITTEE ON ALCOHOL AND OTHER DRUGS REPORT OF THE SUBCOMMITTEE ON ALCOHOL: TECHNOLOGY, PHARMACOLOGY, AND TOXICOLOGY
NATIONAL SAFETY COUNCIL COMMITTEE ON ALCOHOL AND OTHER DRUGS REPORT OF THE SUBCOMMITTEE ON ALCOHOL: TECHNOLOGY, PHARMACOLOGY, AND TOXICOLOGY WASHINGTON, DC FEBRUARY 18, 2008 Subcommittee Co-Chairs: Patrick
More informationA New Methodfor the Rapid A nalysis of Ethanol in Breath, Blood, Urine and Saliva Using the A Icolmeter Evidential Instrument
A New Methodfor the Rapid A nalysis of Ethanol in Breath, Blood, Urine and Saliva Using the A Icolmeter Evidential Instrument T. P. Jones,a A. W. Jones and P. M. Williams INTRODUCTION The introduction
More informationFreeStyle Lite A Blood Glucose Meter That Requires No Coding
Journal of Diabetes Science and Technology Volume 2, Issue 4, July 2008 Diabetes Technology Society SYMPOSIUM Shridhara, Ph.D. Abstract Background: Abbott Diabetes Care introduced the FreeStyle Lite blood
More informationRELIABILITY AND VALIDITY OF BLOOD ALCOHOL CONCENTRATION RELATED TO MEASURED PERFORMANCE DECREMENT
RELIABILITY AND VALIDITY OF BLOOD ALCOHOL CONCENTRATION RELATED TO MEASURED PERFORMANCE DECREMENT Gene G. Rugotzke, Wyoming Public Health Laboratory, Cheyenne, Wyoming, U.S.A.; Robert L. Wilkes, and Robert
More informationLowering of the Alcohol Limits for Drivers in Ireland and the Analytical Impact
Lowering of the Alcohol Limits for Drivers in Ireland and the Analytical Impact Helen Kearns, Pauline Leavy, David Reynolds, Denis Cusack. Medical Bureau of Road Safety, University College Dublin. Abstract
More informationACETONE EXPIRATION AND BREATH-ALCOHOL TESTING. D. Mebs, Ph.D. ; J. Gerchow,*Ph.D. ; and K. Schmidt; Ph.D. SYNOPSIS
ACETONE EXPIRATION AND BREATH-ALCOHOL TESTING k * D. Mebs, Ph.D. ; J. Gerchow,*Ph.D. ; and K. Schmidt; Ph.D. SYNOPSIS Acetone may interfere with breath-alcohol testing using instruments measuring in the
More informationDrinking Drivers Estimates of BAC
Drinking Drivers Estimates of BAC Douglas J. Beirness, Traffic Injury Research Foundation Robert D. Foss, West Virginia University Robert B. Voas, National Public Services Research Institute Karen M. Sprattler,
More informationGamma-glutamyl Transpeptidase Activity Among Convicted Multiple DUI Offenders
Gamma-glutamyl Transpeptidase Activity Among Convicted Multiple DUI Offenders Mary-Ellen Fortini, William C. Cooper, & Robert G. Temer Occupational Health Services, Inc. 125 East Sir Francis Drake Blvd.
More informationAlcoholism markers in their relation to prevention and rehabilitation
Alcoholism markers in their relation to prevention and rehabilitation by M. Staak, R. Iffland Institute of Legal Medicine, University at Cologne, Melatengiirtel 60-62, W - 5000 Köln 30, Germany Alcohol
More informationAccuracy and Precisionof Breath-AlcoholMeasurementsfor a Random Subject in the PostabsorptiveState
CLIN. CHEM. 33/2, 261-268 (1987) Accuracy and Precisionof Breath-AlcoholMeasurementsfor a Random Subject in the PostabsorptiveState G. SImpson The accuracy of estimates of blood-alcohol concentration based
More information510(k) SUBSTANTIAL EQUIVALENCE DETERMINATION DECISION SUMMARY ASSAY ONLY TEMPLATE. Quantitative (Oxide Semiconductor Alcohol Sensor)
510(k) SUBSTANTIAL EQUIVALENCE DETERMINATION DECISION SUMMARY ASSAY ONLY TEMPLATE A. 510(k) Number: k060343 B. Purpose for Submission: New Device for the US market. C. Measurand: Breath Alcohol D. Type
More informationMETHOD VALIDATION CASE
METHOD VALIDATION CASE METHOD VALIDATION PROTOCOL CLIA Regulation 493.1253 (2) 1.Accuracy (closeness to true/comparative method) 2.Precision (reproducibility) 3.Reference Interval 4.Reportable range (linearity,
More informationBeating the Breath Test Increase your Knowledge and Increase Your Wins. Caesars Palace Hotel Las Vegas, NV
Beating the Breath Test Increase your Knowledge and Increase Your Wins Caesars Palace Hotel Las Vegas, NV Breath test cases can be WON!!! CSI Effect Jurors love science The defenses are based in science
More informationForum for Collaborative HIV Research External Validation of CD4 and Viral Load Assays Paris, France June 29, 2007
Forum for Collaborative HIV Research External Validation of CD4 and Viral Load Assays Paris, France June 29, 2007 Thomas J. Spira, M.D. International Laboratory Branch Global AIDS Program Centers for Disease
More informationResearch Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form
Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Gowekar NM, Lawande YS*, Jadhav DP, Hase RS and Savita N. Gowekar Department
More informationCopyright 2013 Crosscutting Concepts, LLC. All Rights Reserved.
Drug use in the USA In 2010, 22.6 million Americans 12 or older (8.9 % of the population) were current illicit drug users. This was an increase over previous years, mostly due to more marijuana use. Drug
More informationMETHOD VALIDATION: WHY, HOW AND WHEN?
METHOD VALIDATION: WHY, HOW AND WHEN? Linear-Data Plotter a,b s y/x,r Regression Statistics mean SD,CV Distribution Statistics Comparison Plot Histogram Plot SD Calculator Bias SD Diff t Paired t-test
More informationMETHOD VALIDATION CASE
METHOD VALIDATION CASE METHOD VALIDATION PROTOCOL CLIA Regulation 493.1253 (2) 1.Accuracy (closeness to true/comparative method) 2.Precision (reproducibility) 3.Reference Interval 4.Reportable range (linearity,
More informationQUALITY ASSURANCE OF BREATH ALCOHOL MEASUREMENTS. Mirela Adelaida ANGHEL; Prof. univ. dr. ing. Fănel IACOBESCU b
QUALITY ASSURANCE OF BREATH ALCOHOL MEASUREMENTS Mirela Adelaida ANGHEL; Prof. univ. dr. ing. Fănel IACOBESCU b a) National Institute of Metrology Romania; b) Romanian Bureau of Legal Metrology, e-mail:
More informationA150 Product Number 31150
PRODUCT INSTRUCTIONS A150 Product Number 31150 FOR THE QUANTITATIVE DETERMINATION OF ALCOHOL IN SALIVA FOR IN VITRO DIAGNOSTIC USE INTENDED USE The OraSure Technologies Q.E.D. Saliva Alcohol Test is intended
More informationEvaluation Report: Eurolyser CRP test (ST0100 and ST0102) on. CUBE analyser (CA0100)
Evaluation Report: Eurolyser CRP test (ST0100 and ST0102) on CUBE analyser (CA0100) Location Location: Eurolyser Diagnostica GmbH Operators: Simone Wieser; Franz Helminger; Michael Gruber Date: July-November
More informationForensic Toxicology. Chapter 17. Mrs. Svedstrup
Forensic Toxicology Chapter 17 Mrs. Svedstrup Forensic Toxicology Toxicology is study of harmful effects of drugs and poisons on living things Forensic toxicology is legal application of toxicology to
More informationAlcohawk Precision. Owner s Manual Q3 Innovations, LLC
Alcohawk Precision Owner s Manual 2005 Q3 Innovations, LLC TABLE OF CONTENTS INTRODUCTION 1 Overview of the Alcohawk Precision 1 Alcohol Testing Theory 1 Description of Testing System 2 Dose-Specific Effects
More informationEXPERIMENT 3 ENZYMATIC QUANTITATION OF GLUCOSE
EXPERIMENT 3 ENZYMATIC QUANTITATION OF GLUCOSE This is a team experiment. Each team will prepare one set of reagents; each person will do an individual unknown and each team will submit a single report.
More informationThe Lion Intoxilyzer 6000 and Lion Intoximeter 3000: comparison of response to Methyl Tertiary Butyl Ether
The Lion Intoxilyzer 6000 and Lion Intoximeter 3000: comparison of response to Methyl Tertiary Butyl Ether David S JOSTY Lion Laboratories pic, Barry, W ales CF63 2BE United Kingdom INTRODUCTION In various
More informationMethod Comparison Report Semi-Annual 1/5/2018
Method Comparison Report Semi-Annual 1/5/2018 Prepared for Carl Commissioner Regularatory Commission 123 Commission Drive Anytown, XX, 12345 Prepared by Dr. Mark Mainstay Clinical Laboratory Kennett Community
More informationEffects of temperature and humidity of inhaled air on the concentration of ethanol in a man's exhaled breath
Clinical Science (1982) 63,441-445 44 1 Effects of temperature and humidity of inhaled air on the concentration of ethanol in a man's exhaled breath A. W. JONES Department of Alcohol and Drug Addiction
More informationUSING THE ACCESS AMH ASSAY IN YOUR LABORATORY
INFORMATION BULLETIN USING THE ACCESS AMH ASSAY IN YOUR LABORATORY ///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
More informationPaul M Williams*, David Honeybourneb, David S Josty*, Alan Mooreb and Keith Butterfield6
Breath alcohol analysis and the lion intoxilyzer 6000: alcohol plateau monitoring in 'normal* subjects, people of large and small stature, and patients with pulmonary disease Paul M Williams*, David Honeybourneb,
More informationDrugs and Driving: Detection and Deterrence
Drugs and Driving: Detection and Deterrence Advisory Group on Drugs in Traffic Report prepared by : Douglas J. Beirness, Ph.D., Barry K. Logan, Ph.D., Philip Swann, Ph.D. Key Messages Drugs are as serious
More informationEvaluation of the Precision and Accuracy of the INRatio 2 Prothrombin Time (PT) Monitoring System
Evaluation of the Precision and Accuracy of the INRatio 2 Prothrombin Time (PT) Monitoring System Grace DeSantis, PhD Lisa Croner, PhD Michealle Havenhill, BA Shari Kipp, BSc (Honours) MBA Inverness Medical,
More informationDetermination of hemoglobin is one of the most commonly
ORIGINAL ARTICLE Multiple-Site Analytic Evaluation of a New Portable Analyzer, HemoCue Hb 201+, for Point-of-Care Testing Sten-Erik Bäck, PhD,* Carl G. M. Magnusson, PhD, Lena K. Norlund, MD, PhD, Henning
More informationPerformance Guide. icheck Iodine The test kit to measure iodine in salt
Performance Guide icheck Iodine The test kit to measure iodine in salt Performance explained This Performance Guide is intended to explain the validity of our test kit icheck Iodine in a nutshell to facilitate
More informationISO-ANALYTICAL. Laboratory Report. δ 2 H (deuterium) and δ 18 O of Doubly Labeled Water (DLW) Analysis: IA Ref. No.: Our LIMS Code.
ISO-ANALYTICAL Laboratory Report Client: Contact(s): Analysis: Your Company Your Name δ 2 H (deuterium) and δ 18 O of Doubly Labeled Water (DLW) IA Ref. No.: Our LIMS Code Your Ref.: From: Your Job Code
More informationDetection of Residual Mouth Alcohol Using Electrochemical Sensors
Detection of Residual Mouth Alcohol Using Electrochemical Sensors Slemeyer, Andreas University of Applied Sciences of Giessen Dept. of Electrical Engineering 1 Wiesenstr. 14, D-35390 Giessen / Germany
More informationOraSure Technologies, Inc Eaton Avenue, Bethlehem, PA Phone: (610) (USA) Fax: (610)
OraSure Technologies, Inc. 1745 Eaton Avenue, Bethlehem, PA 1818-1799 Phone: (61) 882-182 1-8-869-3538 (USA) Fax: (61) 882-183 www.orasure.com SALIVA ALCOHOL TEST Product Instructions for A15 Product Number
More informationA.W. Jones Department of Forensic Toxicology, University Hospital, L ink~ping, Sweden. I Abstracl t. Introduction. Materials and Methods
Lack of Association Between Urinary Creatinine and Ethanol Concentrations and Urine/Blood Ratio of Ethanol in Two Successive Voids from Drinking Drivers A.W. Jones Department of Forensic Toxicology, University
More informationAccuracy and Precision Performance of the Accu-Chek Mobile System. Introduction. Method
Accuracy and Precision Performance of the Accu-Chek Mobile System I. ACCURACY The accuracy of the system was assessed according to ISO 15197. Introduction The purpose of this study was to determine the
More informationPASSIVE TESTING FOR ALCOHOL WHITE PAPER SERIES ABSTRACT
WHITE PAPER SERIES PASSIVE TESTING FOR ALCOHOL ABSTRACT Stricter workplace health and safety regulations now require increased compliance in alcohol related workplace accidents and incidents. While the
More informationGlucoMen LX PLUS blood glucose and blood ketone meter. Accuracy Evaluation to New ISO 15197:2013, with Technical and Specification Data
GlucoMen LX PLUS: Accuracy Evaluations to ISO 15197:2013 with Technical and Specification Data GlucoMen LX PLUS blood glucose and blood ketone meter Accuracy Evaluation to New ISO 15197:2013, with Technical
More informationForensic Toxicology. Forensic Science
Forensic Toxicology Forensic Science Copyright and Terms of Service Copyright Texas Education Agency, 2011. These materials are copyrighted and trademarked as the property of the Texas Education Agency
More informationQ.E.D. Saliva Alcohol Test
Q.E.D. Saliva Alcohol Test A Quantitative Test for the Determination of Equivalent Blood Alcohol Content (BAC) Using a Saliva Sample. Approved by the Federal Department of Transportation (DOT) for Commercial
More informationComparison of Multiple DUI Offenders Selecting Either Antabuse or 12-Step Treatment Programs
Comparison of Multiple DUI Offenders Selecting Either Antabuse or 12-Step Treatment Programs Mary-EIlen Fortini Occupational Health Services 125 East Sir Francis Drake Blvd. Larkspur, CA USA 94939-1860
More informationGlucose Analytical Comparability Evaluation of the YSI 2300 STAT Plus and YSI 2900D Biochemistry Analyzers
Glucose Analytical Comparability Evaluation of the YSI 2300 STAT Plus and YSI 2900D iochemistry Analyzers Life Sciences Data for Life. TM YSI Life Sciences White Paper 91 Authors: Kevin Schlueter, PhD,
More informationGentian Canine CRP Immunoassay Application Note for Abbott Architect * c4000
v2-jan214 Gentian Canine CRP Immunoassay Application Note for Abbott Architect * c4 Intended Use The canine CRP immunoassay on Abbott's Architect c4 is an in vitro diagnostic test for quantitative determination
More informationCHAPTER 33 SCIENTIFIC, CHEMICAL AND BIOLOGIC LABORATORIES AND TESTING PART 2 BLOOD AND BREATH TESTING UNDER THE NEW MEXICO IMPLIED CONSENT ACT
NEW MEXICO DEPARTMENT OF HEALTH SCIENTIFIC LABORATORY DIVISION 700 Camino de Salud N.E. Albuquerque, New Mexico 87106 TITLE 7 HEALTH CHAPTER 33 SCIENTIFIC, CHEMICAL AND BIOLOGIC LABORATORIES AND TESTING
More informationBREATH TESTING FOR ALCOHOL
BREATH TESTING FOR ALCOHOL These materials were prepared by Murray Malcolm of the RCMP Forensic Laboratory - Alcohol Section, Regina, Sask. for the Saskatchewan Legal Education Society Inc. seminar, Ciminal
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(3):289-294 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Simultaneous UV-spectrophotometric estimation of
More informationProblems with Outliers in Breath Alcohol Testing
Problems with Outliers in Breath Alcohol Testing Jo c h e n W ilske Blood alcohol concentration (BAC) and impairment o f driving skills are related so closely, that statutory drink-drive limits are accepted
More informationClinical Chemistry (CHE 221)
Clinical Chemistry (CHE 221) Experiment # 14 Blood Alcohol Determination by Gas Chromatography and by Reaction with Alcohol Dehydrogenase Name Date Performed Date Submitted Partners Name(s) Partners Name(s)
More informationFreeStyle Mini Blood Glucose Results Are Accurate and Suitable for Use in Glycemic Clamp Protocols
Journal of Diabetes Science and Technology Volume 2, Issue 5, September 2008 Diabetes Technology Society CLINICAL APPLICATIONS FreeStyle Mini Blood Glucose Results Are Accurate and Suitable for Use in
More informationInstitute of Forensic Medicine, University of Düsseldorf, FRG
COMPARISON BETWEEN THE RESULTS OF BLOOD AND BREATH ANALYSES OF DWI-DRIVERS Freudenstein, P., Huckenbeck, W., Schmidt, P., Bonte, W. Institute of Forensic Medicine, University of Düsseldorf, FRG SUMMARY
More informationWeek 17 and 21 Comparing two assays and Measurement of Uncertainty Explain tools used to compare the performance of two assays, including
Week 17 and 21 Comparing two assays and Measurement of Uncertainty 2.4.1.4. Explain tools used to compare the performance of two assays, including 2.4.1.4.1. Linear regression 2.4.1.4.2. Bland-Altman plots
More informationFinal Exam Version A
Final Exam Version A Open Book and Notes your 4-digit code: Staple the question sheets to your answers Write your name only once on the back of this sheet. Problem 1: (10 points) A popular method to isolate
More informationHeadspace Gas Chromatography to Quantitate and Identify Volatiles in Liquids
Headspace Gas Chromatography to Quantitate and 1.0 Purpose - This procedure specifies the required elements for calibration and use of the headspace gas chromatograph to determine alcohol (ethanol, methanol,
More informationIn Vitro Diagnostic Glucose Test System
CDRH Final Guidance 2-Page Cover Sheet Guidance for Industry In Vitro Diagnostic Glucose Test System Document issued on: July 6, 1998 U.S. Department Of Health and Human Services Food and Drug Administration
More informationPARALLEL BREATH ALCOHOL CURVES AND BLOOD ALCOHOL CURVES WITH TEST PERSONS UNDER ALCOHOL INFLUENCE
PARALLEL BREATH ALCOHOL CURVES AND BLOOD ALCOHOL CURVES WITH TEST PERSONS UNDER ALCOHOL INFLUENCE O. Griiner, N. Bilzer, M. Kramer Abteilung Rechtsmedizin I, University of Kiel, Hospitalstr. 17/19, 2300
More informationPolymer Technology Systems, Inc. CardioChek PA Comparison Study
Polymer Technology Systems, Inc. CardioChek PA Comparison Study Evaluation Protocol: Accuracy Precision Clinical Correlation PTS Panels Lipid Panel Test Strips For Use in Comparisons to a Reference Laboratory
More informationDEVELOPMENT AND VALIDATION OF COLORIMETRIC METHODS FOR THE DETERMINATION OF RITONAVIR IN TABLETS
Int. J. Chem. Sci.: 8(1), 2010, 711-715 DEVELOPMENT AND VALIDATION OF COLORIMETRIC METHODS FOR THE DETERMINATION OF RITONAVIR IN TABLETS R. PRIYADARSINI *, V. NIRAIMATHI, T. SARASWATHY, V. GOPI and R.
More informationHeadspace Gas Chromatography to Quantitate and Identify Volatiles in Liquids
Headspace Gas Chromatography to Quantitate and 1.0 Purpose - This procedure specifies the required elements for calibration and use of the headspace gas chromatograph to determine alcohol (ethanol, methanol,
More informationSafety Assessment of Hypobromous Acid (220 ppm as Br 2 ) Used as a Beef Carcass Wash
Safety Assessment of Hypobromous Acid (220 ppm as Br 2 ) Used as a Beef Carcass Wash Prepared for: Enviro Tech Chemical Services Modesto, CA Prepared by: Duncan Turnbull, DPhil, DABT Senior Science Advisor
More informationHexagon PSA. Design Verification. Contents
Design Verification Hexagon PSA Contents 1. Function...2 2. Sensitivity, Dynamic Range and Traceability...2 Description of Control Materials...2 Analytical Sensitivity...2 3. Clinical Evaluation...3 Evaluation
More information510(k) SUBSTANTIAL EQUIVALENCE DETERMINATION DECISION SUMMARY ASSAY AND INSTRUMENT COMBINATION TEMPLATE
A. 510(k) Number: k100322 510(k) SUBSTANTIAL EQUIVALENCE DETERMINATION DECISION SUMMARY ASSAY AND INSTRUMENT COMBINATION TEMPLATE B. Purpose for Submission: Clearance of a new device C. Measurand: Whole
More information(a) y = 1.0x + 0.0; r = ; N = 60 (b) y = 1.0x + 0.0; r = ; N = Lot 1, Li-heparin whole blood, HbA1c (%)
cobas b system - performance evaluation Study report from a multicenter evaluation of the new cobas b system for the measurement of HbAc and lipid panel Introduction The new cobas b system provides a point-of-care
More informationPerformance Guide. icheck Chroma 3 The test kit to measure vitamin A in oil
Performance Guide icheck Chroma 3 The test kit to measure vitamin A in oil Performance explained This Performance Guide is intended to explain the validity of our test kit icheck Chroma 3 in a nutshell
More informationPlasma Hemoglobin Determination: Two Manual Methods Compared. An Honors Thesis (HONRS 499) Anne M. Emerick. Sharlene Strahl. Beech Grove, Indiana
Plasma Hemoglobin Determination: Two Manual Methods Compared An Honors Thesis (HONRS 499) by Anne M. Emerick Sharlene Strahl St. Francis Hospital and Health Centers Beech Grove, Indiana Dr. Nancy Behforouz
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD
Translated English of Chinese Standard: GB5009.5-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 5009.5-2016 National food safety standard
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(5):8-14 ISSN: 0976-8688 CODEN (USA): PSHIBD Simultaneous UV-spectrophotometric estimation of bromhexine hydrochloride and
More informationSimple Linear Regression the model, estimation and testing
Simple Linear Regression the model, estimation and testing Lecture No. 05 Example 1 A production manager has compared the dexterity test scores of five assembly-line employees with their hourly productivity.
More informationHarvey M. Cohen, Ph.D. 1. Limitations of the Current Alcohol Testing Procedures, DWI Journal, Law & Science, 1 (1) (1986).
Harvey M. Cohen, Ph.D. Publications Relating to Alcohol and Other Drugs 1. Limitations of the Current Alcohol Testing Procedures, DWI Journal, Law & Science, 1 (1) 55-90 (1986). 2. Blood Alcohol Concentration
More informationElectrolyte-balanced heparin in blood gas syringes can introduce a significant bias in the measurement of positively charged electrolytes
Electrolyte-balanced heparin in blood gas syringes can introduce a significant bias in the measurement of positively charged electrolytes Citation for published version (APA): Berkel, van, M., & Scharnhorst,
More informationREPORT OF THE SUBCOMMITTEE ON ALCOHOL: TECHNOLOGY, PHARMACOLOGY AND TOXICOLOGY
REPORT OF THE SUBCOMMITTEE ON ALCOHOL: TECHNOLOGY, PHARMACOLOGY AND TOXICOLOGY COMMITTEE ON ALCOHOL AND OTHER DRUGS NATIONAL SAFETY COUNCIL Orlando, FL February 15, 1999 Subcommittee Co-Chairs: Patrick
More informationVirtual Lectures Planning Committee Disclosure Summary
Mayo Medical Laboratories Virtual Lectures 2014 MFMER MFMER Virtual Lectures Planning Committee Disclosure Summary As a provider accredited by ACCME, College of Medicine, Mayo Clinic (Mayo School of CPD)
More informationEthanol Clearance Rate as a Function of Age, Gender, and Drinking Practices
Ethanol Clearance Rate as a Function of, Gender, and Drinking Practices H e rb ert M oskow itz, D ary Fiorentino, an d M arcelline B urns Southern California Research Institute, Los Angeles, California,
More informationMouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and
More informationGentian Canine CRP Immunoassay Application Note for scil VitroVet*
v02-jan2014 Gentian Canine CRP Immunoassay Application Note for scil VitroVet* Intended Use The canine CRP immunoassay on scil VitroVet is an in vitro diagnostic test for quantitative determination of
More information3.2A Least-Squares Regression
3.2A Least-Squares Regression Linear (straight-line) relationships between two quantitative variables are pretty common and easy to understand. Our instinct when looking at a scatterplot of data is to
More informationLIPASE liquicolor. Design Verification. Multipurpose Reagent
Design Verification LIPASE liquicolor Multipurpose Reagent CONTENTS 1 Introduction... 2 2 Imprecision... 2 3 Linearity and Detection Limit... 2 3.1 Linearity... 2 3.2 Detection Limit... 3 4 Recovery of
More informationHeadspace Gas Chromatography to Quantitate and Identify Volatiles in Liquids
Headspace Gas Chromatography to Quantitate and 1.0 Purpose - This procedure specifies the required elements for calibration and use of the headspace gas chromatograph to determine alcohol (ethanol, methanol,
More informationSection 3.2 Least-Squares Regression
Section 3.2 Least-Squares Regression Linear relationships between two quantitative variables are pretty common and easy to understand. Correlation measures the direction and strength of these relationships.
More informationDetermination of Calcium in Serum Samples by AAS Using a Fuel Lean Flame
Determination of Calcium in Serum Samples by AAS Using a Fuel Lean Flame Application Note Atomic Absorption Authors Michael J. Sommer Michael G. Rutman Elizabeth Wask-Rotter Howard Wagoner and Edward T.
More informationAS and A-level Biology: Answers to activities in Transition guide
AS and A-level Biology: Answers to activities in Transition guide Activity 1 1. Depending on the organism, or fitness level, s or ms 2. cm 3. km 4. μm 5. g or kg 6. g 7. m 3 Activity 2 1. 6000 kg 2. 4
More informationMEDAK DIST. ANDHRA PRADESH STATE, INDIA. Research Article RECEIVED ON ACCEPTED ON
Page67 Available Online through IJPBS Volume 1 Issue 2 APRIL- JUNE 2011 SIMPLE QUANTITATIVE METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN PUREFORM AND PHARMACEUTICAL DOSAGE FORMS BYUV SPECTROSCOPY
More informationBovine Insulin ELISA
Bovine Insulin ELISA For quantitative determination of insulin in bovine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSBO-E01 Size: 96 wells Version:
More informationMethod (6 to 1000 µg/l Chlorine as Cl 2 ) Digital Titrator
, ABT, DT, 10025 Amperometric Back Titration 1 Scope and Application: For drinking water and wastewater; USEPA accepted for reporting 1 Procedure is equivalent to Standard Method (18th ed.) 4500-Cl C for
More informationo They are usually used in Forensic or Medico-legal practice, Commonly used are Blood Alcohol Concentration (BAC) and Expired Air
1 ETHANOL: UNIVERSITY OF PNG SCHOOL OF MEDICINE AND HEALTH SCIENCES DIVISION OF BASIC MEDICAL SCIENCES DISCIPLINE OF BIOCHEMISTRY AND MOLECULAR BIOLOGY PBL SEMINAR OVERVIEW OF ALCOHOL (ETHANOL & METHANOL)
More informationALCOHOL AND MARIJUANA, A LESS THAN ADDITIVE INTERACTION?
ALCOHOL AND MARIJUANA, A LESS THAN ADDITIVE INTERACTION? Helen Dauncey, Gregory Chesher, John Crawford, Michael Adena, Kim Horne. Department of Pharmacology, University of Sydney, Australia There have
More informationGrand Rapids Effects Revisited: Accidents, Alcohol and Risk
Grand Rapids Effects Revisited: Accidents, Alcohol and Risk H.-P. Krüger, J. Kazenwadel and M. Vollrath Center for Traffic Sciences, University of Wuerzburg, Röntgenring 11, D-97070 Würzburg, Germany ABSTRACT
More information2.1 Determination of Ethanol (Ethyl Alcohol)
Document #: 4981 Page 1 of 13 2.1 Determination of Ethanol (Ethyl Alcohol) 2.1.1 General Description of Method A procedure utilizing a gas chromatograph (GC) for the determination of ethanol. Two quantitative
More informationHemostasis Test Validation, Performance and Reference Intervals
Hemostasis Test Validation, Performance and Reference Intervals Richard A. Marlar, Ph.D. Pathology and Laboratory Medicine Oklahoma City VA Medical Center University of Oklahoma Health Sciences Center
More informationAlcotest 7410 GLC Operator Manual Prepared By: Toxicology Services National Forensic Services (Amended April 2011)
Alcotest 7410 GLC Operator Manual Prepared By: Toxicology Services National Forensic Services (Amended April 2011) Table of Contents Introduction... 1 Physiology of Breath Testing... 2 Description... 3
More informationAlcohol and Tobacco Jeopardy. 10th Grade Health
Alcohol and Tobacco Jeopardy 10th Grade Health Effects of Tobacco / Alcohol Miscellaneous section Pathways of Alcohol Addiction Multiple Choice 100 100 100 100 100 200 200 200 200 200 300 300 300 300 300
More information