SERUM HUMAN PLACENTAL LACTOGEN (HPL) LEVELS IN NORMAL PREGNANCY
|
|
- Stephany Parker
- 6 years ago
- Views:
Transcription
1 SERUM HUMAN PLACENTAL LACTOGEN (HPL) LEVELS IN NORMAL PREGNANCY by G. K. RASTOGI,* F.R.C.P. (E.), M.R.C.P. (Lond.), M. K. SINHA,** M.Sc., S. JAYARAMAN,*** B.V.Sc. and P. K. DEvr,**** M.S., F.R.C.S. It is now well established that human placenta secretes a protein hormone Human Placental Lactogen (HPL), also referred to as placental Somatomarnmotropin. It shows immunological crossreactivity with pituitary growth hormone and possesses both mammotropi.c and somatotropic effects (Jasiomovich and Maclaren, 1962, Kaplan and Grumbach 1968). This hormone is produced by syncytiotrophoblast layer of the placental epithelium (Sciarra et al, 1963). It is present in measurable amounts in early pregnancy and circulating levels in maternal serum progressively increase with advancing pregnancy' (Grumbach et al, 1958, Beck et al, 196S, Samaan et al, 1966). The introduction of the techniques of radioimrnunoassays (Yalow and Berson. *Associate Professor and Head, Department of Endocrinology. **Research Officer. Department of Endocrinology. ***Research Fellow, Depa1 tment of Pharmacology. ****Prof. and Head, Depm tment of Obstetrics and Gynaecology. Fnnn the Departments of Endocrinology, Pharmacology and Obstetrics and Gynaecology, Postgraduate Institute of MediC'al Education and Research, Chandiga.rh. Correspondence: Dr. G. K. Rastogi, Department of Endocrinology, Postgraduate Institute of Medical Education and Research, Chandigarh. Received for publication on ) and improved method for labelling of peptide hormones with radioiodine to achieve high specific activity (Greenwood et al, 1963) have facilitated the measurement of a variety of polypeptide and glycoprotein hormones in the biological fluids with high degree of accuracy and specificity. The present paper describes a double: antibody technique of radioimmunoassay of serum HPL and also summarises our preliminary results in normal pregnant women at different periods of gestation. Material and Method.s Morning venous blood samples were collected from healthy male and female subjects, healthy pregnant women at different duration of pregnancy and the first week after parturi ti<?n. The sera were separated and kept deep frozen at-20 C until assayed. The reagents used in the assay, purified HPL for both standards and iodination and anti-hpl rabbit serum (CT 3399) were kindly supplied by the National Pituitary Agency, NIAMDD, Bethesda, Md; USA. Human placental lactogen was iodinated with (Radiochemical centre, Amersham (UK) by the chloramine-t method of Greenwood et al, (1963). The labelled hormone from the reaction mix-
2 66 JOURNAL OF OBSTETRICS AND GYNAECOLOGY OF INDIA ture was separated by gel chromatography on Sephadex G-50 (1 x 15 em column) equilibrated and eluted with barbitone buffer (0.05 M, ph 8.2). The second antibody (anti-rabbit gamma globulin goat serum, 2nd AB) was raised in our laboratory. A double antibody technique described previously for other hormone assays from this laboratory was used for HPL also (Rastogi et al, 1973 and Rastogi et al, 1973). Protocol of the assay The entire assay was carried out in 10 x 75 mm corning glass test tubes. The reagents were added in the following order. 1. Buffer (0.05 M barbitone, containing 0.3% bovine serum albumin and 0.01% merthiolate, ph 8.2) to make final volume 1 ml ul of standard HPL preparation (range ngjml) or serum samples diluted to 1/ 10 to 1/ 100 in buffer (early pregnancy 1/ 10, mid-pregnancy 1/50 and late pregnancy 1/ ul of diluted hormone free human serum to standard dilution tubes to equalize the protein content with that of unknown sample tubes ul of anti-hpl rabbit serum (1st AB, 1:12,000) HPL (approximately 10,000 counts/ 100 Sec. ( ug HPL) in 100 ul. 6. After 72 hours of incubation at 4 C, 100 ul of non-immune rabbit serum (1: 100) in 0.1 M EDn'A ph 7.8 and 100 ul of 2nd AB (1:6). Twenty four hours later the incubation mixture was centrifuged at 4 C for 15 minutes at 2000 rmp. The supernatant was decanted and the precipitate representing bound 125 I-HPL was counted for 100 seconds in a well type mannual gamma spectrometer at the photopeak oi 12 "!. The standard curve was drawn by plotting the bound HPL (% of total) on the linear scale against the standard dilutions on the log scale along the horizontal axis. From the 7o bound HPL in unknown samples the HPL content of the dilute serum in ng/ ml could be read directly and by multiplying it by the dilution factor the serum HPL levels were obtained as ugj ml serum. The nonspecific radioactivity trapped in the precipitate was assessed from the control tubes containing no 1st AB and it was alwa.ys less than 5%. The purity of the 1 :J5 I-HPL was assessed in each assay by setting up tubes with excess 1st AB. All unknown samples were assayed in duplicate and the standard HPL dilutions in triplicate. The cross-reaction of the anti-hpl rabbit serum with varying concentratiom:. of LH, FSH, TSH, HCG and HGH wa:::. also checked. Routinely in all the assays, aliquots from three pooled sera having different concentrations of HPL were assayed for intra- and inter-assay variations. Results Figure 1 shows the standard curve for HPL. The sensitivity of the assay was 0.1 ng per tube. TSH, LH, FSH, & HCG upto concentrations of 100 mu/ ml, 100 mu/ ml, 100 mu/ml and 1 IU/ ml respectively were not able to significantlor displace HPL from HPL antibody (1st AB). Human growth hormone showed significant cross-reaction only when added in very high concentration, 10 to 20 ng HGH manifesting the displacement effect of 0.2 ng HPL (1:50'-100 ratio). The inter- and intra-assay coefficient of variations were less than 10%. Both in healthy men and women no HPL _, -
3 SERUM HUMAN PLACENTAL LACTOGEN (HPL) LEVELS 67 f# TABLE I Comparative Values of Serum HPL L e v e l~ a! Term and Standard used by Different workers HPL levd No. of Author(s) at or near term (ug/ml) S.D. samples studied Standard HPL preparation Kaplan & Grurnbach ( 1965) 5.6 Beck et al (1965) 25.0 Sarnaan et a/ (1966) 3.3 Spellacy et al (1966) 10.8 Sciarra et a/ (1968) 3.98 Saxena et a/ ( 1968) 19.8 Samaan et al ( 1969) 6.5 Saxena et al ( 1969) 6.8 Singer et a/ (1 970) 8.5 Jasiornovich ( 1970) 8.0 Letchwon h PI o/ ( 1971) 5.6 Present study ( ) 4.06 ± Kaplan CGP-10-5 Beck PPP ± Friesen HPP 50 Leder le C-125 ±3.64 ±6.0 Lederle PP (H) BC Frisen no 67 '1 ± Lederle PPP (H)-1 ± Friesen PPP (H) ±5.0 Lederle PPP (H) ± Lederle PPP-4886 Cll ± NPA, NTAMDD 11 Qjrnl -~-t P ~ - "'~wgw -itml fsi-4.u\,1/ ml. ~ "*~SI'I ftl \,1/lf<l serum HPL levels were undetectable or were negligible (less than 10 ngj ml). Figure 2 shows the serum HPL levels during different periods of pregnancy. :I I L, S ID.Q ;I 0 5 I HH '1'1'3/1111 Fig. 1 Showing the dose response standard surve fo r HPL radioimmunoassay and cross-reaction by HGH, LH, FSH, and TSH. like immunoreactivity was detected. In the first post-partum week, either the f ~2 : ~ : : p. ~.,L. = = -:...'... -t..... I S Zl 2~ "7 40 GESTATION WEEKS Fig_ 2 Serum HPL levels at different weeks of normal pregnancy. Mean and SEM for values every four weeks are also shown. From fifth week onwards human placental lactogen could be detected in the serum of pregnant women and the level of HPL increased progressively with increase in
4 68 JOURNAL OF OBSTETRICS AND GYNAECOLOGY OF INDIA the duration of pregnancy and the maxi mum serum HPL values ( SEM, ugj ml) were observed at term ( 40 weeks). Discussion A double antibody technique for the radioimmunoassajy (RIA) of HPL has been described. This RIA is specific for HPL as there was practically no cross reaction with luteinizing hormone, follicle stimulating hormone, human chorionic gonadotropin and thyroid stimulating hormone. However, only human growth hormone at the concentration of 100 and 200 ngj ml significantly crossreacted with HPL. Since sera were assayed in 1/ 10 or greater dilution, the HGH levels would have to be > 1 ugj ml to be detectable in HPL assays. Such high levels of HGH are not known even in HGH producing tumours. As expected the serum HPL levels in healthy male and non-pregnant female subjects were undetectable and early in the post-partum period also only very low concentration of HPL, still circulating after delivery, was detectable. Consistent detection of HPL in serum as early as 5 weeks of gestation suggests that the sensitivity of the present assay of HPL is similar to that reported by others workers (Beck, et al., 1965 and Kaplan and Grumbach, 1965). In this study the serum HPL levels are similar to those reported by Samaan et al, 1966 and Sciarra et al, 1968 but when compared to results of other groups our values are slightly lower, (Kaplan and Grumbach, 1965; Beck et al, 1865; Spellacy et al, 19Sfi; Saxena et al, 1968; Samaan et al, 1969; Saxena et al, 1969; Singer et al, 1970; J asiomovich et al, 1970 and Letchworth ei al, 1971). As is shown in the table, discrepancy in reported HPL values by different workers is perhaps due to the use of different standards. The value of HPL estimation as a test of placental function and comparison with other conventional tests in normal pregnancy, threatened abortion, and complete abortion is under evaluation. This report provides base line data in north Indian women and would be of value to other laboratories in the country setting up similar assays. Summary A double antibody radioimmunoassay of human placental lactogen (HPL) has been described. Serum HPL levels were estimated in 148 women with normal pregnancy at different weeks of gestation. HPL was detectable in the serum as early as 5 weeks of gestation and its serum levels increased progressively from 0.55 ugj ml (SEM) at 5-8 weeks to at weeks. No HPL could be detected in the serum of healthy non-pregnant women, healthy men or in the first week post-partum. References 1. Beck, P., Parker, M. L. and Daughaday, W. H. : J. Clin Endocr Metab. 25: 1457, Greenwood, F. C., Hunter, W. M. and Glover, J. S.: Biochem J. 89: 114, 1963, 3. Grumbach, M. M., Kaplan, S. L., Sciarra, J. J. and Burr, I. M. : Ann N. Y. Acad Scien 148: 501, J asiomovich, J. B. and Maclaren J. A. Endocrinology 71: 209, Jasiomovich, J. B., Kosor, B., Bocella, L~ Mintz, D. H. and Huchinson, D. L. Obstet. and Gynec. 362: 244, Kaplan, S. L. and Grumbach, M. M.: J. Clin Endocr Metab 25: 1370, Rastogi, G. K., Dash, R. J. and Sinha, M. K. : J. Asso. Phys India 21: 643, Rastogi, G. K., Sinha, M. K., Dash, R. J. and Kannan, V. : J. Assoc. Phys India 21: 183, Letchworth, A. T., Boadrman, R., Bri stow, C. Landon, J. and Chard, T.: J.
5 SERUM HUMAN PLACENTAL LACTOGEN (HPL) LEVELS 69 Obst. Gynec. Brit Common 78: 542, Samaan, N., Yen, S. C. C., Friesen, H. G. and Pearson, 0. H. : J. Clin End ocr Metab 26: 1303, Samaan, N. A., Bradbury, J. I. and Goplerud, G. P.: Am J. Obst. Gynec. 104: 781, Saxena, B. N., Refetoff, S., Emerson, K. and Selenkow, H. A. : Am J. Obst. Gynec. 101: 874, Saxena, B. N. Emerson, K and Selenkow, H. A. : New Eng. J. Med. 281: 225, Sciarra, J. J., Kaplan, S. L. and Grum bach, M. M.: Nature 199: 1005, Sciarra, J. J., Sherwood, L. M., Verma, A. A. and Lundberg, W. B. : Am J. Obst. Gynec. 101: 413, Singer, W., Desjardins, P. and Friesen H. G. : Obst. & Gynec. 36: 222, Spellacy, W. N., Carlson, K. L. and Birk, S. A.: Am J. Obst. Gynec. 96: 1164, Yalow, R. S. and Berson S. A., J. Clin Invest 39: 1157, , '.
(Adams 8c Purves 1958), or LATS-protector (LATS-P) (Adams 8c Kennedy. 1967). The failure of the McKenzie (1958) mouse bioassay to detect LATS in
Department of Endocrinology, Royal Prince Alfred Hospital, and Department of Medicine, University of Sydney, Sydney, Australia THE THYROTROPHIN RECEPTOR IN HUMAN THYROID PLASMA MEMBRANES: EFFECT OF SERUM
More informationDepartment of Obstetrics and Gynecology, School of Medicine, Chiba University, Chiba
Endocrinol. Japon. 1971, 18 (6), 477 `485 Radioimmunoassays for Rat Follicle Stimulating and Luteinizing Hormones KATSUYOSHI SEKI, MITSUNORI SEKI, TERUFUMI YOSHIHARA AND HIDEYASU MAEDA Department of Obstetrics
More informationMetabolic Effects and Disposition in
Journal of Clinical Investigation Vol. 46, No. 1, 1967 Human Placental Lactogen: Studies of Its Acute Metabolic Effects and Disposition in Normal Man * PAUL BECK t AND WILLIAM H. DAUGHADAY t (From the
More informationhcg (Human) CLIA Kit Catalog Number KA assays Version: 01 Intended for research use only
hcg (Human) CLIA Kit Catalog Number KA2799 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationPlasma levels of gonadotropin releasing hormone during menstrual cycle of Macaca radiata
J. Biosci., Vol. 10, Number 4, December 1986, pp. 423-428. Printed in India. Plasma levels of gonadotropin releasing hormone during menstrual cycle of Macaca radiata N. MATHIALAGAN and A. JAGANNADHA RAO
More informationJ. Biosci., Vol. 7, Number 2, March 1985, pp Printed in India.
J. Biosci., Vol. 7, Number 2, March 1985, pp. 123 133. Printed in India. Irreversibility of the interaction of human growth hormone with its receptor and analysis of irreversible reactions in radioreceptor
More informationWHO International Biological Reference Preparations Held and distributed by the WHO International Laboratories for Biological Standards
WHO International Biological Preparations Activin A, human, recombinant. Lyophilized. 5 units / Reagent, 1998 No. 897 49th NIBSC 91/626 98.1882 Arginine vasopressin. Lyophilized. 8.2 IU / Standard, 1978
More informationSee external label 2 C 8 C 96 tests B-HCG (Total) Cat #
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external
More informationRadioimmunoassay Specific for Amino (N) and Carboxyl (C) Terminal Portion of Parathyroid Hormone
Endocrinol. Japon. 1975, 22 (6), 471 `477 Radioimmunoassay Specific for Amino (N) and Carboxyl (C) Terminal Portion of Parathyroid Hormone MASAHIRO TANAKA, KAORU ABE, IsAMu ADACHI KEN YAMAGUCHI, SUMIKO
More information(Received 9th January 1974)
RELEASE OF LH AND FSH IN THE NORMAL INTACT RAM BY SYNTHETIC LH-RF AND THE EFFECT OF PRETREATMENT WITH TESTOSTERONE PROPIONATE C. R. N. HOPKINSON, H. C. PANT and R. J. FITZPATRICK Department of Veterinary
More informationPREPARATION STANDARD MATERIAL HELD AT CODE WHO/BS DOCUMENT. Native hormone purified from urine
WHO International Biological Reference Preparations Held and Distributed by the WHO International Laboratories for Biological Standards ENDOCRINOLOGICAL SUBSTANCES Activin A, human, recombinant, Lyophilized,
More informationFSH (Rodent) ELISA Kit
FSH (Rodent) ELISA Kit Catalog Number KA2330 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationStability of thyroxine and triiodothyronine in
Stability of thyroxine and triiodothyronine in biological fluids LYNN NYE, T. H. YEO, VIVIAN CHAN1, D. GOLDIE2, AND J. From the Department of Chemical Pathology, St Bartholomew's Hospital, London EC] J.
More informationSee external label 96 tests ULTRASENSITIVE THYROID STIMULATING HORMONE (u-tsh) TSH Ultra Sensitive
DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and 116, Woodland Hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com
More informationFertility Diagnostics
Fertility Diagnostics Fertility hormones measured on PATHFAST For internal use only Diagnostics PATHFAST Chemiluminescence-immuno-analyzer 1 Content: page 1. Fertility hormones - general aspects 1.1 Reproductive
More informationThe Third Department of Internal Medicine, University of Tokyo Faculty of Medicine, Hongo, Tokyo 113
Endocrinol. Japon. 1974, 21 (2), 115 ` 119 A Radioimmunoassay for Serum Dehydroepiandrosterone HISAHIKO SEKIHARA, TOHRU YAMAJI, NAKAAKI OHSAWA AND HIROSHI IBAYASHI * The Third Department of Internal Medicine,
More informationPURIFICATION AND ACTION SITES OF A FOLLICLE STIMULATING HORMONE INHIBITOR FROM BOVINE FOLLICULAR FLUID t
PURIFICATION AND ACTION SITES OF A FOLLICLE STIMULATING HORMONE INHIBITOR FROM BOVINE FOLLICULAR FLUID t E. Sato, T. Ishibashi and A. Iritani Kyoto university 2, Kyoto 606, Japan Summary The purification
More informationCorrelation and comparison of Nb 2 lymphoma cell bioassay with radioimmunoassay for human prolactin*
FERTILITY AND STERILITY Copyright 1984 The American Fertility Society Printed in U.8A. Correlation and comparison of Nb 2 lymphoma cell bioassay with radioimmunoassay for human prolactin* Marappa G. Subramanian,
More informationUltra-Sensitive Estradiol lumelisa Catalog No. GWB-AEB745, legacy id (96 Tests)
For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Ultra Sensitive Estradiol (E2) (Chemiluminescence Enzyme Linked Immunosorbent Assay) is used for the ultra sensitive
More informationTSH (Human) ELISA Kit
TSH (Human) ELISA Kit Catalog Number KA0197 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationSee external label 2 C-8 C Σ=96 tests Cat # 3122Z MICROWELL ELISA THYROID STIMULATING HORMONE (TSH) ENZYME IMMUNOASSAY TEST KIT TSH.
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external
More informationEndocrinology of the Female Reproductive Axis
Endocrinology of the Female Reproductive Axis girlontheriver.com Geralyn Lambert-Messerlian, PhD, FACB Professor Women and Infants Hospital Alpert Medical School at Brown University Women & Infants BROWN
More informationThyrotoxicosis in Pregnancy: Diagnose and Management
Thyrotoxicosis in Pregnancy: Diagnose and Management Yuanita Asri Langi email: meralday@yahoo.co.id Endocrinology & Metabolic Division, Internal Medicine Department, Prof.dr.R.D. Kandou Hospital/ Sam Ratulangi
More informationHuman Ultrasensitive Thyroid Stimulating Hormone ELISA Kit
Human Ultrasensitive Thyroid Stimulating Hormone ELISA Kit Catalog No: IRAPKT2026 Lot No: SAMPLE INTENDED USE For the quantitative determination of the thyroid stimulating hormone (TSH) concentration in
More informationLH (Rodent) ELISA Kit
LH (Rodent) ELISA Kit Catalog Number KA2332 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationCarolyn Pheteplace. Department of Obstetrics and Gynecology,
Department of Obstetrics and Gynecology, Harvard Medical School, and Department of Surgery, Peter Bent Brigham Hospital. Boston, Massachusetts, U. S. A. FOLLICLESTIMULATING HORMONE AND LUTEINIZING HORMONE
More informationHuman Thyroid Stimulating Hormone CLIA kit
Human Thyroid Stimulating Hormone CLIA kit Cat. No.:DEEL0223 Pkg.Size:96 tests Intended use For the direct quantitative determination of Thyroid Stimulating Hormone in human serum by chemiluminescence
More informationRADIOIMMUNOASSAY OF THYROID RELATED HORMONES AND TSH IN PRIMARY HYPERTHYROIDISM
RADIOIMMUNOASSAY OF THYROID RELATED HORMONES AND TSH IN PRIMARY HYPERTHYROIDISM Pages with reference to book, From 215 To 219 Farida Agha ( Pakistan Medical Research Council, Research Centre, Karachi.
More informationImmunologic Cross-Reaction Between Luteinizing Hormone and Human Chorionic Gonadotropin
Immunologic Cross-Reaction Between Luteinizing Hormone and Human Chorionic Gonadotropin MELVIN L. TAYMOR, M.D., DONALD A. GOSS, M.D., and ALBERT BUYTENDORP, M.D. RECENTLY a number of reports 2 4 have indicated
More informationPreparation and Validation of Double Antibody Radioimmunoassay for Thyroglobulin (Tg) using Balb/C Mice as Host Animals
Preparation and Validation of Double Antibody Radioimmunoassay for Thyroglobulin (Tg) using Balb/C Mice as Host Animals I.Y. Abdel-Ghany, H.M. Shafik and N.R.A. El-Mouhty Labelled Compounds Dept, Hot Labs.
More informationHuman Thyroid Stimulating Hormone (TSH) ELISA Kit
Catalog No: IRAPKT2025 Human Thyroid Stimulating Hormone (TSH) ELISA Kit Lot No: SAMPLE INTENDED USE For the quantitative determination of thyroid stimulating hormone (TSH) concentration in human serum.
More informationMEASUREMENT OF EARLY CHORIONIC ACTIVITY WITH A RADIOIMMUNOASSAY SPECIFIC FOR HUMAN CHORIONIC GONADOTROPIN FOLLOWING SPONTANEOUS AND INDUCED OVULATION*
Copyright 1974 The American Fertility Society FERTLTY AND STERLTY Vol. 25 No.3 March 1974 Printed in U.S.A. MEASUREMENT OF EARLY CHORONC ACTVTY WTH A RADOMMUNOASSAY SPECFC FOR HUMAN CHORONC GONADOTROPN
More informationCambridge CB2 3EG. ['25I]L-thyroxine. Experiments were performed after 24 hr had elapsed.
J. Physiol. (1971), 212, pp. 447-454 447 With 2 text-ftgurea Printed in Great Britain AN EXAMINATION OF THE EXTENT OF REVERSIBILITY OF THYROXINE BINDING WITHIN THE THYROXINE DISTRIBUTION SPACE IN THE RABBIT
More informationUltra-sensitive Human TSH ELISA Kit. MyBioSource.com
Ultra-sensitive Human TSH ELISA Kit For the quantitative determination of human thyroid stimulating hormone (TSH) concentrations in serum Catalog Number: MBS590037 96 tests FOR LABORATORY RESEARCH USE
More informationFollicle Stimulating Hormone (FSH) ELISA Catalog No (96 Tests)
For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The FSH ELISA kit is used for the quantitative measurement of FSH in human serum or plasma. SUMMARY AND EXPLANATION Follicle-Stimulating
More informationChorionic Gonadotropin Human ELISA Kit
ab108637 Chorionic Gonadotropin Human ELISA Kit Instructions for Use For the qualitative measurement of Human Chorionic Gonadotropin concentrations in serum and urine. This product is for research use
More informationAnti-TSH-Receptor ELISA
Instructions for Use Anti-TSH-Receptor ELISA Enzyme Immuno Assay for the Quantitative Determination of TSH Receptor Autoantibodies (TRAb) in Serum I V D REF EA101/96 12 x 8 2 8 C DLD Gesellschaft für Diagnostika
More informationSuperovulation of Beef Heifers with Follicle Stimulating Hormone or Human Menopausal Gonadotropin: Acute Effects on Hormone Secretion
Superovulation of Beef Heifers with Follicle Stimulating Hormone or Human Menopausal Gonadotropin: Acute Effects on Hormone Secretion A.S. Leaflet R1362 Acacia A. Alcivar, graduate research assistant,
More informationHuman Obestatin ELISA
K-ASSAY Human Obestatin ELISA For the quantitative determination of obestatin in human serum and plasma Cat. No. KT-495 For Research Use Only. 1 Rev. 081309 K-ASSAY PRODUCT INFORMATION Human Obestatin
More informationTSH (Human) CLIA Kit. Catalog Number KA assays Version: 01. Intended for research use only.
TSH (Human) CLIA Kit Catalog Number KA2795 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationLH (Canine) ELISA Kit
LH (Canine) ELISA Kit Catalog Number KA2292 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationDiabetologia 9 Springer-Verlag 1982
Diabetologia (1982) 22:327-332 Diabetologia 9 Springer-Verlag 1982 Metabolic Control and Serum Hormone Levels in Relation to Retinopathy in Diabetic Pregnancy J. Larinkari 1, L. Laatikainen 1, T. Ranta
More informationLH (Bovine) ELISA Kit
LH (Bovine) ELISA Kit Catalog Number KA2280 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationChanges in rat ovaries of specific binding for LH, FSH and prolactin during the oestrous cycle and pregnancy
Changes in rat ovaries of specific binding for LH, FSH and prolactin during the oestrous cycle and pregnancy K. W. Cheng Department ofphysiology, Faculty of Medicine, University of Manitoba, Winnipeg,
More informationParathyroid Hormone: Secretion and Metabolism In Vivo
Proc. Nat. Acad. Sci. USA Vol. 68, No. 12, pp. 2986-2991, December 1971 Parathyroid Hormone: Secretion and Metabolism In Vivo (human/bovine/gel filtration/radioii catheterization) JOEL F. HABENER, DAVID
More informationHuman Follicle-Stimulation Hormone ELISA Kit
Catalog No: IRAPKT2001 Human Follicle-Stimulation Hormone ELISA Kit Lot No: SAMPLE INTENDED USE For the quantitative determination of follicle-stimulation hormone (FSH) concentration in human serum. FOR
More informationThe Effect of Fetal Thyroidectomy on Thyroid Hormone Metabolism in Maternal and Fetal Sheep
Pediat. Res. 7: 870-877 (1973) Fetus thyroid iodothyronine uterus The Effect of Fetal Thyroidectomy on Thyroid Hormone Metabolism in Maternal and Fetal Sheep A. ERENBERG, K. OMORI, W. OH, AND D. A. FISHER
More informationRat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit
Rat Mullerian Inhibiting Substance/Anti-Mullerian hormone, MIS/AMH ELISA kit Catalog No. E0228r (96 tests) Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
More informationTSH Receptor Autoantibody ELISA
Li StarFish S.r.l. Via Cavour, 35-20063 Cernusco S/N (MI), Italy Tel. +39-02-92150794 - Fax. +39-02-92157285 info@listarfish.it -www.listarfish.it TSH Receptor Autoantibody ELISA Product Data Sheet Cat.
More informationSuperovulation of Beef Heifers with Follicle Stimulating Hormone or Human Menopausal Gonadotropin: Acute Effects on Hormone Secretion
Beef Research Report, 1996 Animal Science Research Reports 1997 Superovulation of Beef Heifers with Follicle Stimulating Hormone or Human Menopausal Gonadotropin: Acute Effects on Hormone Secretion Acacia
More informationFish follicle-stimulating hormone, FSH ELISA Kit
Fish follicle-stimulating hormone, FSH ELISA Kit Catalog No: E0830f 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationPP per rabbit). Standards for the assay were prepared. method (Hunter and Greenwood, 1962): 1 jug of pure HPP was iodinated with 0 5 uci carrier free
Gut, 1976, 17, 940-944 Distribution and release of human pancreatic polypeptide T E ADRAN, S R BLOOM, M G BRYANT, J M POLAK, PH HETZ, AND A J BARNES From the Department of Medicine and Histochemistry,
More informationPregnancy Test on a Urine Sample. Amal Alamri
Pregnancy Test on a Urine Sample Amal Alamri Molecular characteristics of HCG hcg is a glycoprotein composed of two subunits, αand β, held together by ionic and hydrophobic forces. The α - subunit is a
More informationOvarian Function in the Preovulatory Rabbit.2
BOLOGY OF REPRODUCTON 17, 34-38 (1977) Ovarian Function in the Preovulatory Rabbit.2 CHUNG H. WU, LUS BLASCO, GEORGE L. FLCKNGER and GEORGE MKHAL Endocrine Section, Department of Obstetrics and Gynecology,
More informationMeasurement of Digoxin by Radioimmunoassay
ANNALS OF CLINICAL AND LABORATORY SCIENCE, Vol. 7, No. 1 Copyright 1977, Institute for Clinical Science Measurement of Digoxin by Radioimmunoassay PHILIP R. WALSH, Ph.D.,* J. FREDERICK CRAWFORD, Ph.D.,*
More informationFSH (Human) ELISA Kit
FSH (Human) ELISA Kit Catalog Number KA0213 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationFree beta hcg quantitative assay is designed for quantitative measurement of chorionic Gonadotropin free beta subunit in human serum.
User's Manual Free ß - HCG Free beta hcg quantitative assay is designed for quantitative measurement of chorionic Gonadotropin free beta subunit in human serum. Catalogue Nr : KAPD1793 96 tests Revision
More informationStability of Stopped thyroid hormones in Enzyme Linked Immunosorbent Assay
Stability of Stopped thyroid hormones in Enzyme Linked Immunosorbent Assay Kayode J. Adebayo Department of Chemical Pathology, Faculty of Clinical Sciences, College of Medical Sciences Ambrose Alli University
More informationEliKine Free Thyroxine (ft4) ELISA Kit
EliKine Free Thyroxine (ft4) ELISA Kit Booklet Item NO. KET0005 Product Name EliKine Free Thyroxine (ft4) ELISA Kit ATTENTION For laboratory research use only. Not for clinical or diagnostic use TABLE
More informationab Chorionic Gonadotropin beta Human ELISA Kit
ab178633 Chorionic Gonadotropin beta Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma. This product
More informationLH (Horse) ELISA Kit. Catalog Number KA assays Version: 01. Intended for research use only.
LH (Horse) ELISA Kit Catalog Number KA2302 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationPreparation of 125I-thyroxine and 125I-triiodothyronine of High Specific Activity for the Radioimmunoassay of Serum Total T4 and T3
Ann. din. Biochem. 13 (1976) 364-368 Preparation of 125I-thyroxine and 125I-triiodothyronine of High Specific Activity for the Radioimmunoassay of Serum Total T4 and T3 VANESSA R. THURLOW AND HILARY J.
More informationIVD Revised 22 July, 2008
INTENDED USE The DRG One Step HCG Pregnancy Test is an in vitro diagnostic test for the qualitative detection of human chorionic gonadotropin (hcg) in urine. The test is a two-site immunoassay employing
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E-8600 TSH rat ELISA 1. INTRODUCTION 1.1 Intended use The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research
More informationENDOCRINOLOGY COORDINATION OF PHYSIOLOGICAL PROCESSES:
ENDOCRINOLOGY COORDINATION OF PHYSIOLOGICAL PROCESSES: -In a living organism there must be coordination of number of physiological activities taking place simultaneously such as: movement, respiration,
More informationMeasurement of free insulin concentrations: the influence of the timing of extraction of insulin antibodies
Diabetologia (1985) 28: 831-835 Measurement of free insulin concentrations: the influence of the timing of extraction of insulin antibodies I. Hanning, P. D. Home and K. G. M. M. Alberti Department of
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E8600 TSH rat ELISA INTRODUCTION INTENDED USE The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research use only.
More informationStudy of thyroid profile during pregnancy
Original Research Article Study of thyroid profile during pregnancy Raghav Nepalia 1*, Renuka Z Lal 2 1 Sr. Demonstrator, 2 Assistant Professor Department of Biochemistry, RNT Medical College, Udaipur,
More informationand Luteinizing Hormone as Measured by Radioimmunoassay Correlated with Sexual Development in Hypopituitary Subjects
Serum Follicular - Stimulating Hormone and Luteinizing Hormone as Measured by Radioimmunoassay Correlated with Sexual Development in Hypopituitary Subjects ROBERT PENNY, THOMAS P. FOLEY, JR., and ROBERT
More informationMICROWELL ELISA LUTEINIZING HORMONE (LH) ENZYMEIMMUNOASSAY TEST KIT LH ELISA. Cat # 4225Z
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external
More informationHCG (human chorionic gonadotropin); Novarel Pregnyl (chorionic gonadotropin); Ovidrel (choriogonadotropin alfa)
Federal Employee Program 1310 G Street, N.W. Washington, D.C. 20005 202.942.1000 Fax 202.942.1125 5.08.09 Subject: HCG Page: 1 of 5 Last Review Date: June 19, 2015 HCG Powder, Novarel, Pregnyl, Ovidrel
More informationNeutralizing and Non-neutralizing Antibodies
Neutralizing and Non-neutralizing Antibodies to Bovine Thyroid-Stimulating Hormone and its Subunits GIDON N. BEALL, INDER J. CHOPRA, DAVID H. SOLOMON JOHN G. PIERCE, and JAMES S. CORNELL From the Department
More informationCross-Reactivity with Three Other Hormones
Antibody to Human Follicle-Stimulating Hormone: Cross-Reactivity with Three Other Hormones SHELDON SCHLAFF, SAUL W. ROSEN, and JESSE RoTH From the Clinical Endocrinology Branch, National Institute of Arthritis
More informationModel Answer. M.Sc. Zoology (First Semester) Examination Paper LZT 103 (Endocrinology)
Model Answer M.Sc. Zoology (First Semester) Examination-2013 Paper LZT 103 (Endocrinology) Section A 1. (i) d (ii) b (iii) b (iv) c (v) c (vi) a (vii) c (viii) a (ix) d (x) b Section B Q.2 Answer Hormonal
More informationC-Peptide I and II (Rat) ELISA
C-Peptide I and II (Rat) ELISA For the quantitative determination of rat C-peptide in plasma, serum, urine, and cell culture supernatant Please read carefully due to Critical Changes, e.g., blot plate
More informationReal Pregnancy or Uncontrolled Hypothyroidism?
Real Pregnancy or Uncontrolled Hypothyroidism? Christie G. Turin 1,MD; Anisha Gupta 1, MD; Susan Samson 1,2, MD; Mandeep Bajaj 1,2, MBBS Baylor College of Medicine, Houston, TX 1 ; CHI-St. Luke s Episcopal
More informationSee external label 2 C-8 C Σ=96 tests Cat # 3171Z. Free Estriol. Cat # 3171Z. Enzyme Linked Immunosorbent Assay
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external
More informationEstimation of the Secretion Rate of Thyrotropin in Man *
Journal of Clinical Investigation Vol. 46, No. 6, 1967 Estimation of the Secretion Rate of Thyrotropin in Man * W. D. ODELL,t R. D. UTIGER, J. F. WILBER, AND P. G. CONDLIFFE (From the Endocrinology and
More informationGeneral Protocol for RK CGRP (Rat, Mouse) - RIA Kit. (range: pg/ml)
General Protocol for RK-015-09 CGRP (Rat, Mouse) - RIA Kit (range: 10-1280 pg/ml) introduction This kit is designed to measure a specific peptide and its related peptides by a competitive radioimmunoassay.
More informationRadioimmunoassay for Measurement of
Radioimmunoassay for Measurement of Triiodothyronine in Human Serum INDER J. CHOPRA, DAvm H. SOLOMON, and GILDON N. BEALL From the Department of Medicine, Harbor General Hospital, Torrance, California
More informationTSH ELISA Kit Medical Device Licence No.: 16419
TSH ELISA Kit Medical Device Licence No.: 16419 Enzyme immunoassay kit for the quantitative determination of TSH concentration in serum. Catalog Number: SL100305 Catalog Number: SL100306 Catalog Number:
More informationHYPERTHYROIDISM AND PREGNANCY. (Report of a case and Review of Literature) K. VASISHTA,* M.D. A. N. GuPTA,** M.D., D.G.O.
HYPERTHYROIDISM AND PREGNANCY (Report of a case and Review of Literature) by K. VASISHTA,* M.D. and A. N. GuPTA,** M.D., D.G.O. The association of pregnancy and hyperthyroidism is uncommon as concep tion
More informationDetermination of serum thyroxine using a resin sponge technique
J. clin. Path. (1967), 2, 89 Determination of serum thyroxine using a resin sponge technique JOHN A. KENNEDY AND DENIS M. ABELSON From the Royal Infirmary, Glasgow, and the Graduate Hospital, the University
More informationDELFIA Tb-N1 DTA Chelate & Terbium Standard
AD0029P-1 (en) 1 DELFIA Tb-N1 DTA Chelate & AD0012 Terbium Standard For Research Use Only INTRODUCTION DELFIA Tb-N1 DTA Chelate is optimized for the terbium labeling of proteins and peptides for use in
More informationVGKC-Autoantibody Assay RIA
Instructions for Use VGKC-Autoantibody Assay RIA 125I-Radio Immuno Assay for the Quantitative Determination of Antibodies to the Voltage-Gated Potassium Channel (VGKC) in Serum I V D REF RA115/25 25 2
More informationCortisol (Sheep) ELISA Kit
Cortisol (Sheep) ELISA Kit Catalog Number KA0919 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the
More informationA31-year-old woman (gravida 2, para 1,
CME Practice CMAJ Cases Persistent mild increase of human chorionic gonadotropin levels in a 31-year-old woman after spontaneous abortion Jianing Chen, Sheri-Lee Samson MD, James Bentley MD, Yu Chen MD
More informationMouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and
More informationGARY S. KLEDZIK LIONEL CUSAN CLAUDE AUCLAIR, PH.D. PAUL A. KELLY, PH.D. FERNAND LABRIE, M.D., PH.D.*
FERTILITY AND STERILITY Copyright 1978 The American Fertility Society Vol. 3, No.3, September 1978 Printed in U S.A. INHIBITION OF OVARIAN LUTEINIZING HORMONE (LH) AND FOLLICLE-STIMULATING HORMONE RECEPTOR
More informationChemiluminescence Immunoassay Enzyme Immunassay (CLIA) TSH. Cat #
DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external
More informationTHYROID FUNCTION EVALUATION IN PATIENTS WITH INCREASED OR DECREASED THYROXINE-BINDING PROTEIN
THE AMEBICAN JOURNAL OF CLINICAL PATHOLOGY Vol. 50, No. 3 Copyright 1968 by The Williams & Wilkins Co. Printed in U.S.A. THYROID FUNCTION EVALUATION IN PATIENTS WITH INCREASED OR DECREASED THYROXINE-BINDING
More informationMechanisms of precocious puberty induced in male rats by
Mechanisms of precocious puberty induced in male rats by pituitary grafts R. Aguilar, C. Bellido, J. E. S\l=a'\nchez-Criadoand E. Aguilar Department of Physiology, Faculty of Medicine, Cordoba University,
More informationTokyo Women's Medical College, Shinjukuku,
Tohoku J. exp. Med., 1982, 138, 319-328 Insulin-Receptor Autoantibody Detected by the Human Placental Membrane Method: Six Patients with Insulin-Receptor Autoantibody in Japan YASUE OMORI, HIRATA Diabetes
More informationLaboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and 1gM
J. clin. Path., 1976, 29, 1121-1126 Laboratory diagnosis of rheumatoid arthritis: a solid phase radioassay for IgG and 1gM antiglobulins L. J. NINHAM, F. C. HAY, AND I. M. ROITT From the Department of
More informationAnti 21 - Hydroxylase Assay RIA
Instructions for Use Anti 21 - Hydroxylase Assay RIA Addison s Disease, Autoimmune Polyglandular Syndrome 125I-Radio Immuno Assay for the Quantitative Determination of Antibodies against 21-Hydroxylase
More informationSupplementary Information. Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food
Supplementary Information Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena and Debendra K. Sahoo* Biochemical
More informationKey words: Collagen synthesis - N-Terminal peptide of type III procollagen - Tumor marker - Liver cancer - Liver cirrhosis
[Gann, 75, 130-135; February, 1984] HIGH CONCENTRATIONS OF N-TERMINAL PEPTIDE OF TYPE III PROCOLLAGEN IN THE SERA OF PATIENTS WITH VARIOUS CANCERS, WITH SPECIAL REFERENCE TO LIVER CANCER Terumasa HATAHARA,
More informationThyroid-Stimulating Activity and Chorionic Gonadotropin
Thyroid-Stimulating Activity and Chorionic Gonadotropin BRUCE C. NIsULA and JEAN-MARIE KETELSLEGERS From the Reproduction Research Branch, National Institute of Child Health and Human Development, National
More informationEffects of Growth Hormone on Testicular Testosterone Production In Vitro in Transgenic Mice
Southern Illinois University Carbondale OpenSIUC Honors Theses University Honors Program 12-1989 Effects of Growth Hormone on Testicular Testosterone Production In Vitro in Transgenic Mice Angela Susan
More informationGUIDELINES: This is an investigative study with no applicable guidelines.
CITATION: Handa R, 2014. Terbuthylazine: An oral (gavage) study to assess the effects on the hormoneinduced luteinizing hormone surge in ovariectomized female Wistar rats. College of Medicine, The University
More information