International Journal of Applied Ayurved Research ISSN: PREPARATION OF NITYODITA RASA IN THREE BATCHES
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1 International Journal of Applied Ayurved Research ISSN: PREPARATION OF NITYODITA RASA IN THREE BATCHES Bodhakar Kishor N. 1 Khaparde Prakash H. 2 Khiyani Rajkumar. M. 3 1 Lecturer,Dept.of Rasashastra-Bhaishajya kalpana, Bhausaheb Mulak Ayurved Mahavidyalaya, Nandanvan, Nagpur, Maharashtra. Pincode , India. 2 Dean, Govt. Ayurved College,Madhuban, Osmanabad (M.S.), India. 3 Associate Professor, Department of Rasashastra-Bhaishajya kalpana, Govt. Ayurved College, Nagpur, Maharashtra. ABSTRACT: Among all the formulation described for arsharoga in Rasashastra text, Nityodita Rasa is the better herbomineral formulation. Nowadays market sample of this Rasaushadhi is not available, so preparation for standardization of Nityodita rasa was very difficult task.this is the formulation very effective and potent for vatakaphaj arsha and jirna raktarsha. The basic reference regarding Nityodita rasa under the arsha rogadhikara was found in Rasendrasara samgraha. For standardization three batches of Nityodita rasa were prepared. Rasasindur mainly used in vatakaphaja arsha, abhrakabhasma poses tissue restoration, regulates dhatu pariposhana, hence can be useful in vatakaphaj arsha and jirna raktarsha for better improvement. In jirna raktarsha atrophy, degeneration of muscles and nerves at anal region causes painful condition, where lohabhasma gives relief from such condition. Main property of tamrabhasma is lekhana, hence useful in jirna raktarsha. Vatsanabha possesses the properties like jwarashamaka, swedala hence can be used in kaphanubandhi arsha. Bhallataka mainly acts in vatakaphaj arsha and used in kaphaj agnimandya janya arsha. Suranakanda is best dravya in arsha. Because of that these were the ingredients taken for preparation of Nityodita rasa. Nityodita rasa vati were prepared without disturbing proportions in three batches. For concrete establishment of standard operating procedure for the preparation of Nityodita rasa, more extensive study with preparation of this kalpa by both conventional and modern method by adopting various references should be needed. Also animal trial of such a wonderful drug should be needed for efficacy of drug in arsharoga. Keywords: Rasaushadhi, Nityodita Rasa, Standardization, Arsharoga. INTRODUCTION: Now a day s medicines). Rasaushadhi (Herbomineral Rasaushadhi (Herbomineral drugs) becomes more and more popular, because of their uniqueness, acts in low doses, highly potential, don t having annoying taste, gives quick result and hence having edge drugs) helps ayurvedic practitioners to compete with modern medicine, because it acts in low doses, more potent, gives quick and permanent relief from disease. But still more ayurvedic practitioners doesn t use over the herbal medicines. In this modern Rasaushadhi s because of lack of era Ayurvedic practice becomes tough job, because the competition is with modern medicine which gives quick result, poses emergency medicines. Peoples also want quick relief though they easily ignored Ahara, Vihar and Pathyapathya. Thus can t rely only on Kashtaushadhi (Herbal knowledge, daring and risky methods of their preparations. There are various types of formulations available in Rasaushadhi text on Arsharoga (Piles). Various types of choorna, vati, gutika, rasa, asavarishta are available for this disease. Arsharoga is one of the painful and hectic for human being.
2 Those who are sufferer know the painful stroke of such disease. This disease highly found in non-vegetarian peoples. It is also found in delivered ladies and those peoples who had sitting job carriers. Among all the formulation described in Rasashastra text Nityodita rasa is more potent than other formulations for vatakaphaj arsha and jirna raktarsha. Vati of 250 mg were prepared and by same way three batches of Nityodita rasa without disturbing their proportion prepared for standardization. This Rasaushadhi naturally gives quick result in less time, especially acts in vatakaphaj arsha and jirna raktarsha (Bleeding piles). Also it isn t routine drug and market preparation of this drug not available nowadays. The preparation of such a drug in limited time is one of the great task. But the faith in Rasashastra supported to make such formulation. MATERIAL AND METHODS: Preparation of Nityodita Rasa in three batches All the ingredients needed for Nityodita rasa preparation were taken according to their Grahyalakshana before shodhan process and then prepared bhasma of minerals. Reference: Rasendrasarasamgraha 2/10-11 (Arsharogachikitsa) Equipments: 1) Khalvayantra 2) stirrer, 3) measuring instrument 4) measuring cylinder 5) cloth 6) steel plate, etc. Ingredients 1 : 1. Rassindur - 05 gms 2. Abhrakabhasma - 05 gms 3. Lohabhasma - 05 gms 4. Tamrabhasma - 05 gms 5. Shuddha Vatsanabha (aconitum ferox) - 05 gms 6. Shuddha Gandhaka - 05 gms 7. Shuddha Bhallataka (semicarpus anacardium) - 30 gms 8. Bhavanadravya - Surankanda swarasa -150 ml (Amorphophyllus campanulatus) Procedure: Rasasindur 2 (kupipakva rasayana) was prepared by equal amount of shuddha parada and shuddha gandhaka kajjali that is samguna kajjali as per classical text reference. Abhrakabhasma was prepared by putapaka procedure and total 40 puta given. First 3 puta were given by guda and kalamisora 3 and then 37 puta were given by arkapatra swarasa, dashmula kwatha and kumara swarasa bhavana 4 as per classical text reference. Lohabhasma 5 was prepared by putapaka procedure and total 17 puta given. Marana of loha was done by shuddha hingula and triphala kwatha bhavana procedure as per classical text reference. Tamrabhasma 6 was also prepared by putapaka procedure and total 16 puta given. Marana of tamra was done by samguna kajjali and jambiri nimbu swarasa bhavana as per classical reference. Gandhak shodhana 7 was done by godugdha and goghrita as per classical reference. Vatsanabha shodhana 8 and Bhallataka shodhana 9 did according to classical reference. Then all ingredients measured accurately and sieved through the cloth. Suranakanda swarasa was prepared by putapaka method and kept in a measuring cylinder. First of all Rasasindur was taken in khalva and then Abhrakabhasma, Lohabhasma, Tamrabhasma mixed with it and triturated (mardana) well. Thereafter the shuddha Vatsanabha powder, shuddha Gandhaka and lastly shuddha Bhallataka powder were added and triturated well. Then IJAAR VOLUME III ISSUE II MAY-JUNE 2017
3 Suranakanda swarasa bhavana 10 was given for 3 days and the mixture was triturated till it became thick and able to form vati. Then vati each of weight 2 ratti (250 mg average) was prepared by applying ghruta over palm, dried and packed in air tight container. It was labeled as Batch A. Same procedure was applied and Nityodita Rasa prepared for 2 times and labeled as Batch B and Batch C respectively. Duration 3 days Precautions: All ingredients measured accurately. Suranakanda swarasa should be added slowly. Mardana was done till thick mass formed. The vati should be uniform in size. Apply goghrita over palm during swarasa preparation and vati formation. OBSERVATIONS AND RESULTS: Consistency - rounded, hard Colour - grayish black Odour - nirgandha (odourless) Taste - niswadu (tasteless) Observations of Nityodita Rasa preparation: All bhasma prepared by classical text reference and parikshana was done as per ayurvedic bhasma pariksha and with the help modern tools. The bhasma and other ingredients sieved through cloth were mixed easily. After adding Suranakanda swarasa, thick and pishchhila (smooth) paste was formed. After three days of bhavana and mardana, vati were formed easily. The vati were smooth in touch and grayish black in colour. Average weight of batch A, batch B and batch C vati were 238 mg, 261 mg and 239 mg respectively which is near about 250 mg in all three batches. Table 1: Nityodita rasa ayurvedic parikshan Product Shabda Sparsha Rupa Rasa Gandha Nityodita Smooth, Alpa Grayish black, Niswadu Nirgandha Rasa - bhangura rounded in shape Table 2: Raw drugs study of Nityodita rasa ingredients. Sample Test Value Mercury Assay as Hg %w/w Sulphur Assay as Sulphur %w/w Mica Aluminium as Al 2 O 3 Iron as Fe 2 O 3 Silica as SiO 2 Magnesium as MgO K 2 O content % w/w 2.61 % w/w % w/w % w/w 3.82 % w/w Iron Assay as Fe % w/w Copper Assay as Cu % w/w Impure Vatsanabha Total alkaloids 0.45 % w/w IJAAR VOLUME III ISSUE II MAY-JUNE 2017
4 Impure Bhallataka Foreign matter Loss on Drying Total ash Acid insoluble ash Water soluble extractive Alcohol soluble extractive Fatty Acids Unsaponifiable matter Phenolic constituents 0.84 % w/w 2.47 % w/w 3.76 % w/w 0.41 % w/w 6.14 % w/w % w/w % w/w 2.31 % w/w Positive Table 3: Analysis of vishadravya after shodhana Vishadravya Test Value Shodhita Vatsanabha Total Alkaloids 0.08 % w/w Shodhita Bhallataka Foreign matter Loss on Drying Total ash Acid insoluble ash Water soluble extractive Alcohol soluble extractive Fatty Acids Unsaponifiable matter Phenolic constituents 0.89 % w/w 3.10 % w/w 3.95 % w/w 0.44 % w/w 5.11 % w/w % w/w % w/w 1.86 % w/w Negative Table 4: Analysis of Rasasindur and Bhasma during in process study 11 S.N. Test Rasasindur Value of Value of Value of Abhrakabhasma Lohabhasma Tamrabhasma 1 Loss on drying Total ash Acid Insoluble Ash 4 Iron as Fe (% w/w) 5 Copper as Cu Mercury as Hg Sulpher as S Table 5: Analysis of Nityodita Rasa in three batches. Sr. No. Test Batch Batch B Batch C 1 Disintegration Time (min.) IJAAR VOLUME III ISSUE II MAY-JUNE 2017
5 2 Loss on drying w/w) 3 Total Ash Acid Insoluble Ash Acid Soluble Ash 6 Water Insoluble Ash 7 Water Soluble Ash 8 Water Soluble Extractive 9 Alcohol soluble Extractive Mercury as Hg 12 Total Sulphur as S 13 Iron as Fe Copper as Cu AverageWeight (grams) DISCUSSION: Three batches of cording to indications. Nityodita Rasa is a Nityodita rasa were prepared without disturbing the proportion of ingredients and duration for standardization of drug, because kharaliya rasayana in which Rasasindur is kupipakva rasayana as an ingredient and all the ingredients were mixed in the this is not mentioned in API. Mar- kharala and triturated. Suranakanda ket preparation of this drug is not available nowadays. Nityodita rasa is a complex formulation as it contains Rasasindur, swarasa was used as bhavanadravya which slowly added to the mixture till it became wet and triturated. Suranakanda bhasmas like Abhraka, Loha, swarasa is sticky, hence acts as binding Tamrabhasma, vishadravyas like agent. Also it acts as anuloman and acts in Vatsanabha and Bhallataka and arsha (piles), hence taken in Nityodita Suranakanda swarasa bhavana. The kalpa was prepared according to reference of rasa as bhavanadravya. The mixing and bhavana procedure was done for 3 days in Rasendrasarasamgraha with specific each batch. It should be cautiously used in methods of shodhana and marana ac- jirnaraktarsha, because it contains IJAAR VOLUME III ISSUE II MAY-JUNE 2017
6 Bhallataka which is ushna, tikshna 12 in nature. So it should be used with pittashamak anupana and ghrita in jirnaraktarsha. Average weight of batch A, batch B and batch C vati were 238 mg, 261 mg and 239 mg respectively which is near about 250 mg in all three batches. CONCLUSION: In the text, Nityodita rasa is mentioned as a remedy for all types of arsha, but regarding action and contains of drug it is mainly useful in vatakaphaj arsha, jirna raktarsha 13 and its complication like raktarshajanya agnimandya and pandu. Quality of final product depends on the raw drug selection. For the preparation of drug the raw material was selected according to grahyalakshanas and on the basis of analysis in laboratory. Analysis of raw material suggests it is of good quality. It concludes that although a criteria for raw material selection is subjective, but it is of great significance.there are so many formulations available in the text for the treatment of Arsharoga, but Nityodita rasa is one of the best formulation of Rasashastra mentioned in the text. Nityodita Rasa is the combination of Kupipakva rasayana and Kharaliya rasayana containing Rasasindur, Bhasmas like Abhraka, Loha, Tamrabhasma and Vishadravyas like Vatsanabha, Bhallataka. In Nityodita Rasa, Triphala kwatha was used for Lohabhasma preparation, Because it enhances Pachana and absorption of Loha, also relieves malavashtambha (constipation) caused by lohabhasma which is very usefull in Nityodita rasa. Suranakanda swarasa is used as bhavanadravya which was prepared by putapaka method, mainly used for the purpose of Anuloman and property of Surankanda is arshoghna 13. Nityodita rasa is one of the best formulation in the ancient Rasashastra. With the help of various test analysis of drug done by modern instruments and after clinical trials may able to show reality of mercurial drugs, while the modern medicinal peoples are discussing about side effects of such drugs. Nityodita rasa plays a vital role in chronic bleeding piles (jirna raktarsha) and vatakaphaj arsha. For concrete establishment of standard operating procedure for the preparation of Nityodita rasa, more extensive study with preparation of this kalpa by both conventional and modern method by adopting various references should be needed. ACKNOWLEDGEMENT: Thankful to honorable Dean, Dr. Prakash H. Khaparde, G.A.C., Osmanabad, our honorable Guide Dr. R. M. Khiyani, Asso. Prof., Rasashastra-Bhaishajyakalpana Dept., Govt. Ayurved College, Nagpur and all Teaching staff of Rasashastra-B.K. department for their friendly guidance, inspiration, kind cheerfulness, invaluable advice & moral support during entire course of our post graduate studies and for shown us way at our weaker points and for their generous co-operation, research freedom and ceaseless interest in our research work. Here releasing the limitations of language and words while acknowledging gratitude towards love, care, affection, encouragement of beloved wife Preeti, family members and friends. REFERENCES: 1. Indradeo Tripathi, Rasendrasarsamgrah, Siddhinandan Mishra, Chaukhamba Orientalia publication fourth edition 2006 Varanasi, Adhyay 2 Arshachikitsa 2/10, Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 6/ , Pg IJAAR VOLUME III ISSUE II MAY-JUNE 2017
7 3. Dr. Siddhinandan Mishra, Ayurvediya Rasashastra, Dattatrey Anant Kulakarni, Chaukhamba Orientalia publication 2013 Varanasi, Abhrakprakaran, Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 10/56-64, Pg Vagbhatacharya, Rasaratna samucchaya, Dattatray Anant Kulkarni, Meharchand Lachhamandas publication 2010 Varanasi, adhyay 5/ , Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 17/25, Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 8/7-12, Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 24/19-22, Pg Sadanand Sharma, Rasatarangini, publication 2007 Delhi, Tarang 24/ , Pg Indradeo Tripathi, Rasendrasarsamgrah, Siddhinandan Mishra, Chaukhamba Orientalia publication fourth edition 2006 Varanasi, Adhyay 2 Arshachikitsa 2/11, Pg Honwad Sudhindra V., editor Handbook of Standardization of Ayurvedic Formulations, 1 st ed. Chaukhamba Orientalia 2012 Varanasi, Pg Agnivesha, Charakasamhita part II, Pt. Kashinath Shastri, Chaukhamba Sanskrit sansthan publication sixth edition 2000 Varanasi, chikitsasthan 1/17 Pg Dr. A. Deshpande, Dr. S. Ranade, Dr. R. Jawalagekar, Dravyagun Vigyan part II, M. Nandurkar, Anmol Prakashan 1998 Pune, Pg 935. Corresponding Author: Dr. Kishor Bodhakar N, Lecturer,Dept.of Rasashastra- Bhaishajya kalpana, Bhausaheb Mulak Ayurved Mahavidyalaya, Nandanvan, Nagpur, Maharashtra. Pincode , India. dr.k.bodhakar@gmail.com Source of support: Nil Conflict of interest: None Declared Cite this Article as : [Bodhakar Kishor et al : Preparation of Nityodita Rasa in Three Batches] : IJAAR VOLUME III ISSUE II MAY-JUNE 2017 PAGE No: IJAAR VOLUME III ISSUE II MAY-JUNE 2017
8 398 IJAAR VOLUME III ISSUE II MAY-JUNE 2017
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