The effects of PGS/PGT-A on IVF outcomes
|
|
- Kristopher Parsons
- 5 years ago
- Views:
Transcription
1 The effects of PGS/PGT-A on IVF outcomes Raoul Orvieto M.D. - Department of Obstetrics and Gynecology, Chaim Sheba Medical Center, Ramat Gan, Israel - The Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
2 2 Franasiak et al 2014
3 3
4 4 (2013)
5
6 6
7 Assumptions Every potentially viable embryo will develop to the blastocyst stage in vitro Mosaicism does not exist in embryos The testing is 100% accurate/precise without false (+)(-) results The biopsy procedure does not reduce the viability of the embryo 7
8 8 PGS
9 Technical errors/ mosaicism (2016) 37 blastocyst-stage abnormal embryos from eight patients underwent re-biopsy. 4 embryos were initially analyzed by acgh then with SNP array; 6 of them were initially with SNP array then NGS; 27 of them were initially analyzed by acgh then with NGS. 9/27 (33 %) of embryos originally reported to be aneuploid, upon repeat assessment, were found to be euploid. 9
10 Technical errors/ mosaicism 11 aneuploidy embryos- re-biopsy - 37 specimens (i) inter-laboratory congruity (ii)intra-embryo congruity of multiple embryo biopsies in a single laboratory Only 2/11 (18.2 %) embryos were identically assessed at two PGS laboratories; 4/11 (36.4 %), on repeat analysis were chromosomally normal, 2 mosaic normal/abnormal, 5/11 (45.5 %) completely differed in reported aneuploidies. In intra-embryo analyses, 5/10 (50 %) differed 10 between biopsy sites.
11 Technical errors/ mosaicism X 10 (35.7%) of the 28 biopsies (24 trophectoderms and 4 inner cell masses) 11 revealed mosaicism or inconclusive results. 11
12 Technical errors/ mosaicism One would need a biopsy of 27 cells to assure that the probability of obtaining only euploid cells in a biopsy is less than 5% if the fraction of euploid cells in the embryo is r=0.9 The probability that a biopsy shows no mosaicism as a function of the fraction of euploid cells r = N1/N (N1 is the number of euploid cells, and N the total number of cells) 12
13 Technical errors/ mosaicism (2017) Biopsies with increased relative DNA content impact clinical outcomes. Possible explanations: Diminished accuracy of the euploid diagnosis, resulting from either technical error or biologic error 13 Mechanical impact of the biopsy itself.
14 14 Technical errors/ mosaicism
15 15 False Positive
16 Technical errors/ mosaicism False Negative X 16
17 (2016) Abnormal cells within the ICM were significantly more likely to undergo apoptosis than those within the TE. Hence, abnormal TE cells often remained viable, albeit depleted relative to their normal counterparts.? 17
18 18 PGS
19 RCT 1 0 outcome- Cumulative live birth rates (fresh+thawed) Screened (PGS) Non-screened Non-screened blastocyst blastocyst cleavage-stage embryo N=100 N=100 N=100 19
20 (2012) Blastulation rate 47%
21 RCT 1 0 outcome- Cumulative live birth rates (fresh+thawed) Screened (PGS) Non-screened Non-screened blastocyst blastocyst cleavage-stage embryo N=100 N=100 N=100 Blastulation rate (47%) 47 Blast 47 Blast 100 Cleavage - stage 21
22 (2017) Reprogenetics (US Labs) database of NGS analyzed embryos. 43%
23 RCT 1 0 outcome- Cumulative live birth rates (fresh+thawed) Screened (PGS) Non-screened Non-screened blastocyst blastocyst cleavage-stage embryo Blastulation rate (47%) 47 Blast 47 Blast 100 Cleavagestage Euploidy rate (43%) 20.2 Blast 47 Blast 100 Cleavagestage 23
24 (2013) 65.5% 47.2% 24
25 50% 28.9% 25
26 RCT 1 0 outcome- Cumulative live birth rates (fresh+thawed) Screened (PGS) Non-screened Non-screened blastocyst blastocyst cleavage-stage embryo Blastulation rate (47%) 47 Blast 47 Blast 100 Cleavage-stage Euploidy rate (59%) 20.2 Blast 47 Blast 100 Cleavage-stage Sustained implantation rate 65.5% 47.2% % LIVE BIRTHS 13.2% 22.2% % 26
27 (2012) 45.2% vs 56.0% 27
28 Donors, average age:25.5 ± 3.03 yrs (range: yrs) 28 68%
29 1 0 outcome- Cumulative live birth rates (fresh+thawed) Screened blastocyst (PGS) Non-screened blastocyst N=10 N= N=7 N=10 Sustained implantation rate 65.5% 47.2% LIVE BIRTHS N=4.58 N=4.72
30 (2016) PGS Controls P Cycle (all) All aneuploid embryos No Blastocysts Euploid transfers 60 41/60 (68%) per transfer Age (yrs) Clinical intrauterine gestation 21.5% (41/191) 49.5% <0.001 < /191 (21.5%) per started cycle LBR <
31 TIME TO PREGNANCY Months/cycles Cycle: OIR OPU (1) 2 3 PGS 65.5% Non-PGS 47.2% = X0.655= = = X0.472= = = X0.472= =
32 Fertil Steril Mar;109(3): Patients participating in the RCT are generally favorably responding subjects who have produced blastocysts for biopsy and analysis. A broader selection of patients with randomization at cycle start rather than blastulation would more appropriately address the applicability of wider use of this technology. The randomized trials were performed in centers with broad and deep experience in embryo biopsy and specimen preparation. The ability to expand these techniques to centers with less experience has yet to be established. The extremely challenging questions of false-positive testing, embryonic damage, and loss of euploid embryos between day 3 and blastulation remain unanswered. 32
33 Other important considerations about PGT-A that must be addressed by further research include: cost-effectiveness; the role and effect of cryopreservation, time to pregnancy, utility in specific subgroups (such as recurrent loss, prior implantation failure, advanced maternal age, etc.); cumulative success rates over time; and total reproductive potential per intervention. Unfortunately, at the time of this publication, there are currently very few randomized trials registered to elucidate these answers. Large, prospective, well-controlled studies evaluating the combination of multiple approaches (genomics, time-lapse imaging, transcriptomics, proteomics, metabolomics, etc.) At for enhanced present, embryo however, selection applicable there is in insufficient a more inclusive IVF evidence population to are needed recommend to determine not the only routine the effectiveness, use but of also blastocyst the safety and biopsy potential with risks of these technologies. PGT-A aneuploidy will likely be part testing of a future in multidimensional all infertile approach patients. to embryo screening and selection. 33
34 34 (2018)
35 THANK YOU 35 35
Increase your chance of IVF Success. PGT-A Preimplantation Genetic Testing for Aneuploidy (PGS 2.0)
Increase your chance of IVF Success PGT-A Preimplantation Genetic Testing for Aneuploidy (PGS 2.0) What is PGT-A? PGT-A, or Preimplantation Genetic Testing for Aneuploidy (PGS 2.0), is a type of genomic
More informationIVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW
IVF AND PREIMPLANTATION GENETIC TESTING FOR ANEUPLOIDY (PGT-A) WHAT THE COMMUNITY PHYSICIAN NEEDS TO KNOW Jon Havelock, MD, FRCSC, FACOG Co-Director - PCRM Disclosure No conflict of interest in relation
More informationComprehensive Chromosome Screening Is NextGen Likely to be the Final Best Platform and What are its Advantages and Quirks?
Comprehensive Chromosome Screening Is NextGen Likely to be the Final Best Platform and What are its Advantages and Quirks? Embryo 1 Embryo 2 combine samples for a single sequencing chip Barcode 1 CTAAGGTAAC
More informationResults of the Virtual Academy of Genetics (VAoGEN) questionnaire on Mosaicism in PGS
Results of the Virtual Academy of Genetics (VAoGEN) questionnaire on Mosaicism in PGS Ariel Weissman, MD IVF Unit, Dep. Ob/Gyn Wolfson Medical Center, Holon Sackler Faculty of Medicine, Tel Aviv University
More informationNEXCCS. Your guide to aneuploidy screening
NEXCCS Your guide to aneuploidy screening GROWING FAMILIES What is comprehensive chromosome screening? Comprehensive chromosome screening (CCS), also known as preimplantation genetic screening (PGS) or
More informationChromosomal Aneuploidy
The Many Advantages of Trophectoderm Biopsy Compared to Day 3 Biopsy for Pre- Implantation Genetic Screening (PGS) Mandy Katz-Jaffe, PhD Chromosomal Aneuploidy Trisomy 21 Fetus Aneuploidy is the most common
More informationComprehensive chromosome screening and embryo biopsy: advantages and difficulties. Antonio Capalbo, PhD Italy
Comprehensive chromosome screening and embryo biopsy: advantages and difficulties Antonio Capalbo, PhD Italy Disclosure Antonio Capalbo, PhD GEERA, Reproductive medicine centers GEETYX, molecular genetics
More informationTargeted qpcr. Debate on PGS Technology: Targeted vs. Whole genome approach. Discolsure Stake shareholder of GENETYX S.R.L
Antonio Capalbo, PhD Laboratory Director GENETYX, reproductive genetics laboratory, Italy PGT responsible GENERA centers for reproductive medicine, Italy Debate on PGS Technology: Targeted vs. Whole genome
More informationNew perspectives on embryo biopsy, not how, but when and why PGS
New perspectives on embryo biopsy, not how, but when and why PGS Kangpu Xu, PhD Director, Laboratory of Preimplantation Genetics Center for Reproductive Medicine Weill Cornell Medical College of Cornell
More informationDiagnosis of parental balanced reciprocal translocations by trophectoderm biopsy and comprehensive chromosomal screening
Diagnosis of parental balanced reciprocal translocations by trophectoderm biopsy and comprehensive chromosomal screening Lian Liu, MD Co-Authors: L. W. Sundheimer1, L. Liu2, R. P. Buyalos1,3, G. Hubert1,3,
More informationINSIDE IVF: HOW SCIENCE CARES FOR PATIENTS DR DEIRDRE ZANDER-FOX MONASH IVF GROUP HDA GRAND ROUND OCTOBER 31 ST 2018
INSIDE IVF: HOW SCIENCE CARES FOR PATIENTS DR DEIRDRE ZANDER-FOX MONASH IVF GROUP HDA GRAND ROUND OCTOBER 31 ST 2018 IVF-THE ULTIMATE GOAL FERTILISATION EMBRYO CLEAVAGE AND DEVELOPMENT POSITIVE HCG POSITIVE
More informationNew methods for embryo selection: NGS and MitoGrade
New methods for embryo selection: NGS and MitoGrade Santiago Munné, PhD US: Livingston, Los Angeles, Chicago, Portland, Miami / Europe: Barcelona (Spain), Oxford (UK), Hamburg (Germany) / Asia: Kobe (Japan),
More informationScientifically advanced. Personally accessible.
Scientifically advanced. Personally accessible. EmbryVu. Advanced preimplantation genetic screening that can help you find the path to pregnancy. The power to decide When you are going through treatment
More informationThe ASRM Committee Opinion on PGT for Aneuploidy Our Conclusions and How We Drew Them. Alan Penzias, MD
The ASRM Committee Opinion on PGT for Aneuploidy Our Conclusions and How We Drew Them Alan Penzias, MD Chair, Practice Committee American Society for Reproductive Medicine Development of US National Guidelines:
More informationNothing more controversial than PGS
Nothing more controversial than PGS Norbert Gleicher, MD M e d i c a l D i r e c t o r a n d C h i e f S c i e n t i s t, C e n t e r F o r H u m a n R e p ro d u c t i o n, N e w Yo r k, N Y P r e s i
More informationPGS & PGD. Preimplantation Genetic Screening Preimplantation Genetic Diagnosis
1 PGS & PGD Preimplantation Genetic Screening Preimplantation Genetic Diagnosis OUR MISSION OUR MISSION CooperGenomics unites pioneering leaders in reproductive genetics, Reprogenetics, Recombine, and
More informationUNDERSTANDING THE GENETIC HEALTH OF EMBRYOS
UNDERSTANDING THE GENETIC HEALTH OF EMBRYOS What is preimplantation genetic testing for aneuploidy? (an abnormal number of chromosomes; PGT-A) is a testing technique that can help choose embryos that appear
More informationNon-invasive methods of embryo selection
Non-invasive methods of embryo selection Liow Swee Lian O & G Partners Fertility Centre Gleneagles Hospital SINGAPORE Introduction More physiological laboratory procedures and culture systems have significantly
More informationDr Guy Gudex. Director Repromed. 17:00-17:30 Recent Advances in Fertility Management
Dr Guy Gudex Director Repromed 17:00-17:30 Recent Advances in Fertility Management Recent Advances in Fertility Management Practice Nurses Programme NZMA GP CME June 2018 Dr Guy Gudex ART in NZ -2014 ACART
More informationApplication of OMICS technologies on Gamete and Embryo Selection
Application of OMICS technologies on Gamete and Embryo Selection Denny Sakkas, Ph.D. Scientific Director, Boston IVF Waltham, MA, USA THE FUTURE ROLE OF THE EMBRYOLOGIST WILL FOCUS ON PROVIDING OUR PATIENTS
More informationDiagnostic Techniques to Improve the Assessment of Human IVF Embryos: Genomics and Proteomics
Diagnostic Techniques to Improve the Assessment of Human IVF Embryos: Genomics and Proteomics Mandy G Katz-Jaffe Introduction A fundamental component of assisted reproductive technologies (ART) is the
More informationUnderstanding eggs, sperm and embryos. Marta Jansa Perez Wolfson Fertility Centre
Understanding eggs, sperm and embryos Marta Jansa Perez Wolfson Fertility Centre What does embryology involve? Aims of the embryology laboratory Creation of a large number of embryos and supporting their
More information@ CIC Edizioni Internazionali. Origin and mechanisms of aneuploidies in preimplantation embryos
Review article Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives Antonio Capalbo 1 Cristina Poggiana 1 Cristina Patassini 1 Anna Cecchele 1 Emiliano Scepi 1
More informationCIC Edizioni Internazionali. Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives. Summary.
Mini-review Preimplantation genetic screening: definition, role in IVF, evolution and future perspectives Antonio Capalbo 1 Cristina Poggiana 2 Cristina Patassini 2 Anna Checchele 2 Emiliano Scepi 2 Danilo
More informationSingle Embryo Transfer
Single Embryo Transfer Rachel Cutting Principal Embryologist Chair Association of Clinical Embryologists Summary Background to change in UK policy National data Strategies Embryo selection Practical requirements
More informationDisclosure. Dagan Wells University of Oxford Oxford, United Kingdom
Disclosure Dagan Wells University of Oxford Oxford, United Kingdom Disclosure Declared to be member of the advisory board, board of directors or other similar groups of Illumina Objectives Consider Aneuploidy
More informationExplaining the Purpose of PGT-A. Nathan R. Treff PhD, HCLD(ABB) Chief Science Officer Clinical Laboratory Director
Explaining the Purpose of PGT-A Nathan R. Treff PhD, HCLD(ABB) Chief Science Officer Clinical Laboratory Director Disclosures Cofounder, Shareholder and CSO, Genomic Prediction Inc Director, Genomic Prediction
More informationComprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study
ORIGINAL ARTICLES: ASSISTED REPRODUCTION Comprehensive chromosome screening is highly predictive of the reproductive potential of human embryos: a prospective, blinded, nonselection study Richard T. Scott
More informationCongreso Nacional del Laboratorio Clínico 2016
Actualización en Screening Genético Preimplantacional Maria Giulia Minasi Center for Reproductive Medicine European Hospital Rome, Italy Aneuploidy rate can reach 60% in human embryos Aneuploidy increases
More informationIndications for chromosome screening Dagan Wells, PhD, FRCPath dagan.wells@obs-gyn.ox.ac.ukgyn.ox.ac.uk Chromosome imbalance (aneuploidy) Uncontroversial data The incidence of aneuploidy Aneuploidy is
More informationThe Impact of ESHRE 2017 on Japanese Fertility Practice
The Impact of ESHRE 2017 on Japanese Fertility Practice This resource is supported by an educational grant from Merck KGaA, Darmstadt, Germany. The GWHA was interested in the opinions of practicing clinicians
More informationBlastocentesis: innovation in embryo biopsy
Blastocentesis: innovation in embryo biopsy L. Gianaroli, MC Magli, A. Pomante, AP Ferraretti S.I.S.Me.R. Reproductive Medicine Unit, Bologna, Italy Bologna, 8-11 May 2016 www.iiarg.com www.sismer.it 2013
More informationPreimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC
Preimplantation Genetic Testing Where are we going? Genomics Clinical Medicine Symposium Sept 29,2012 Jason Flanagan, MS,CGC Overview Discuss what PGD and PGS are Pt examples What we have learned Where
More informationDr Guy Gudex. Gynaecologist and Fertility Specialist Repromed. 9:05-9:30 Advances in Assisted Reproduction What s New?
Dr Guy Gudex Gynaecologist and Fertility Specialist Repromed 9:05-9:30 Advances in Assisted Reproduction What s New? Rotorua GP CME June 2016 Advances in Assisted Reproduction-What s new? Dr Guy Gudex
More informationPreimplantation Genetic Testing
Protocol Preimplantation Genetic Testing (40205) Medical Benefit Effective Date: 01/01/14 Next Review Date: 09/14 Preauthorization No Review Dates: 09/11, 09/12, 09/13 The following Protocol contains medical
More informationPreimplantation Genetic Testing (PGT) Fresh and Frozen Embryos Process, Risk, and Consent
Preimplantation Genetic Testing (PGT) Fresh and Frozen Embryos Process, Risk, and Consent PGT analysis is offered to patients that seek to identify a chromosomal abnormality in their embryos prior to initiating
More informationShould ICSI be implemented during IVF to all advanced-age patients with non-male factor subfertility?
Farhi et al. Reproductive Biology and Endocrinology (2019) 17:30 https://doi.org/10.1186/s12958-019-0474-y RESEARCH Open Access Should ICSI be implemented during IVF to all advanced-age patients with non-male
More informationSame Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method
Same Day, Cost-Effective Aneuploidy Detection with Agilent Oligonucleotide array CGH and MDA Single Cell Amplification Method Presenter: Dr. Ali Hellani, Founder, Viafet Genomic Center, Dubai Wednesday,
More informationFertility 101. About SCRC. A Primary Care Approach to Diagnosing and Treating Infertility. Definition of Infertility. Dr.
Dr. Shahin Ghadir A Primary Care Approach to Diagnosing and Treating Infertility St. Charles Bend Grand Rounds November 30, 2018 I have no conflicts of interest to disclose. + About SCRC State-of-the-art
More informationUSA: Livingston, NJ. PGD for infertility. Europe: Barcelona, Spain Oxford, UK Hamburg, Germany. Asia: Kobe, Japan. South America: Lima, Peru
PGD for infertility Santiago Munné USA: Livingston, NJ Europe: Barcelona, Spain Oxford, UK Hamburg, Germany Asia: Kobe, Japan South America: Lima, Peru The majority of embryos with good morphology are
More informationSNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation carrier and normal blastocysts
J Assist Reprod Genet (2016) 33:1115 1119 DOI 10.1007/s10815-016-0734-0 TECHNOLOGICAL INNOVATIONS SNP array-based analyses of unbalanced embryos as a reference to distinguish between balanced translocation
More informationProfessional societies, global organizations, Article 16 of the United Nations Declaration. UPDATE Fertility
UPDATE Fertility G. David Adamson, MD Dr. Adamson is Founder and CEO of Advanced Reproductive Care, Inc (ARC Fertility); Clinical Professor, ACF, at Stanford University School of Medicine; and Associate
More informationPre-implantation genetic diagnosis and pre-implantation genetic screening: two years experience at a single center
Original Article Obstet Gynecol Sci 2018;61(1):95-101 https://doi.org/10.5468/ogs.2018.61.1.95 pissn 2287-8572 eissn 2287-8580 Pre-implantation genetic diagnosis and pre-implantation genetic screening:
More informationAccuracy of assessing embryo ploidy of human embryos with PGS in association with IVF
Accuracy of assessing embryo ploidy of human embryos with PGS in association with IVF Norbert Gl eicher, MD M e d i ca l D i re c t o r A n d Chief Scientist, C e n t e r Fo r H u m a n Re p ro d u c t
More informationINDICATIONS OF IVF/ICSI
PROCESS OF IVF/ICSI INDICATIONS OF IVF/ICSI IVF is most clearly indicated when infertility results from one or more causes having no other effective treatment; Tubal disease. In women with blocked fallopian
More informationDo it Once, Do it Right
Do it Once, Do it Right Craig Reisser Andrea Speck-Zulak Families Through Surrogacy 2016 Founded in 1989 - more than 25 years building families patients from 40 countries One of the largest IVF clinics
More informationPOSEIDON s stratification of Low prognosis patients in ART and its new proposed marker of successful outcome: The WHY, the WHAT, and the HOW
POSEIDON s stratification of Low prognosis patients in ART and its new proposed marker of successful outcome: The WHY, the WHAT, and the HOW Sandro C. Esteves, MD., PhD. I. The WHY The management of patients
More informationHold On To Your Dreams
Hold On To Your Dreams Dr. Michael Kettel Dr. Sandy Chuan 1. THE BASICS OF IVF & EMBRYO DEVELOPMENT 2. IVF ADD-ONS - MYTH VS. SCIENCE IN VITRO FERTILIZATION 1. Ovarian Stimulation 2. Egg Retrieval 3. Create
More informationbut it still needs a bit of work
but it still needs a bit of work jc@embryos.net Reprogenetics ART Institute of Washington Life Global Principle investigator of cytoplasmic transfer series (1996-2001) Is there an alternative to MRT? Lessons
More informationBuilding robust time-lapse models. Kirstine Kirkegaard The Fertility Clinic Aarhus University Hospital
Building robust time-lapse models Kirstine Kirkegaard The Fertility Clinic Aarhus University Hospital Time-lapse as a diagnostic test Morphology Limited information Dependent on timing Subjective Validated
More informationMyths About Success Rates Do they truly reflect quality of care
Myths About Success Rates Do they truly reflect quality of care Barry Behr PhD., HCLD Professor and IVF Laboratory Director Division of Reproductive Endocrinology and Infertility Dept OB/GYN Stanford University
More information2014 CRB Trophectoderm Biopsy Workshop. Jean M. Popwell, PhD, HCLD
2014 CRB Trophectoderm Biopsy Workshop Jean M. Popwell, PhD, HCLD TE Biopsy Discussion Biopsy equipment and supplies Dish workflow Which blastocyst to biopsy? Biopsy Videos The approach is everything!
More informationPGS Embryo Screening
PGS Embryo Screening Contents What are chromosomes? 3 Why should I consider chromosome testing of my embryos? 3 Embryo testing using preimplantation genetic screening (PGS) 4 How does PGS and the chromosome
More informationL6: DuoStim: the alternative of oocytes/embryos accumulation programs Carlo Alviggi
L6: DuoStim: the alternative of oocytes/embryos accumulation programs Carlo Alviggi Italy What is the aim of IVF? What is the measure of success in IVF? Cumulative live birth rate per started cycle Live
More informationMosaicism in human embryos: Etiology and pregnancy outcome Santi Munné
Mosaicism in human embryos: Etiology and pregnancy outcome Santi Munné disclosure - Chief Scientific Officer of CooperGenomics - Founder @ Reprogenetics (PGS/PGD company), sold to Cooper - Founder @ Recombine
More informationShould Every Embryo be Screened or Frozen? What does the evidence say?
Should Every Embryo be Screened or Frozen? What does the evidence say? acgh (Lab 1) 45,XY,-8 qpcr (Lab 2) SNP array (Lab 3) 46,XY Richard T. Scott, Jr, MD, HCLD Clinical and Scientific Director, Reproductive
More informationScientific and Clinical Advances Advisory Committee Paper
Scientific and Clinical Advances Advisory Committee Paper Paper title Paper number SCAAC(06/15)07 Meeting date 10 June 2015 Agenda item 7 Author Information/decision Resource implications Implementation
More informationEmbryo transfer and Luteal phase support
Embryo transfer and Luteal phase support PATCHARADA AMATYAKUL, M.D. DEPARTMENT OF OBSTETRICS AND GYNECOLOGY FACULTY OF MEDICINE NARESUAN UNIVERSITY Embryo Transfer http://www.regionalfertilityprogram.ca/program-embryotransfer.php
More informationNew Innovations and Technologies:
New Innovations and Technologies: How and When in the Fertility Clinic? Prof Darren K Griffin (Biosciences); Prof Sally Sheldon (Law) Centre for Interdisciplinary Studies of Reproduction (CISoR) University
More informationEmbryoCellect TM. Pre-implantation Genetic Screening Kit TECHNICAL INFORMATION
EmbryoCellect TM Pre-implantation Genetic Screening Kit TECHNICAL INFORMATION Aneuploidy Whole chromosome aneuploidy has been shown to affect all chromosomes in IVF embryos. Aneuploidy is a significant
More informationBlastocyst Morphology Holds Clues Concerning The Chromosomal Status of The Embryo
Original Article Blastocyst Morphology Holds Clues Concerning The Chromosomal Status of The Embryo Rita de Cassia Savio Figueira, M.Sc. 1, Amanda Souza Setti, B.Sc. 1,, Daniela Paes Almeida Ferreira Braga,
More informationFreeze-All Policy: Is It Right for Everyone?
Transcript Details This is a transcript of an educational program accessible on the ReachMD network. Details about the program and additional media formats for the program are accessible by visiting: https://reachmd.com/online-education/non-certified-non-accredited/freeze-all-policy-it-righteveryone/9879/
More informationWhat s New in IVF? Dr. Rachael Knight Melbourne IVF
What s New in IVF? Dr. Rachael Knight Melbourne IVF Many promising new treatments in IVF -How do we choose??? Evidence? Wait for the systematic review Takes time depends on the quality of the primary studies
More informationCase report: Pregnancy After Vitrification of Biopsied Human Blastocysts Previously Frozen by the Slow Method
Case report: Pregnancy After Vitrification of Biopsied Human Blastocysts Previously Frozen by the Slow Method Batwala, M., Wilding, M., Create Fertility, Cheapside, London, UK Dalapati, T., University
More informationProgesterone and clinical outcomes
Synchronization of Slowly Developing Embryos Restores Implantation Success Richard T. Scott, Jr, MD, HCLD Clinical and Scientific Director, Reproductive Medicine Associates of New Jersey Professor and
More informationAneuploidies: the embryo point of view
Aneuploidies: the embryo point of view MC Magli, A. Pomante, AP Ferraretti, L. Gianaroli S.I.S.Me.. eproductive Medicine Unit, Bologna, Italy www.iiarg.com www.sismer.it Bologna, May 8-11, 2016 1 46 chromosomes?????
More informationDevelopment of new comprehensive
Development and validation of an accurate quantitative real-time polymerase chain reaction based assay for human blastocyst comprehensive chromosomal aneuploidy screening Nathan R. Treff, Ph.D., a,b Xin
More informationOriginal Policy Date
MP 2.04.77 Preimplantation Genetic Testing Medical Policy Section OB/Gyn/Reproduction Issue 12:2013 Original Policy Date 12:2013 Last Review Status/Date Reviewed with literature search/12:2013 Return to
More informationPreimplantation genetic diagnosis: polar body and embryo biopsy
Human Reproduction, Vol. 15, (Suppl. 4), pp. 69-75, 2000 Preimplantation genetic diagnosis: polar body and embryo biopsy Luca Gianaroli SISMER, Via Mazzini 12, 40138 Bologna, Italy Scientific Director
More informationEffect of ovarian stimulation on oocyte quality and embryonic aneuploidy: a prospective, randomised controlled trial
FULL PROJECT TITLE: Effect of ovarian stimulation on oocyte quality and embryonic aneuploidy: a prospective, randomised controlled trial (STimulation Resulting in Embryonic Aneuploidy using Menopur (STREAM)
More informationDifferences in chromosome abnormalities in eggs and embryos between fertility centers Santi Munné
Differences in chromosome abnormalities in eggs and embryos between fertility centers Santi Munné disclosure None (lol) Except: - Chief Scientific Officer of CooperGenomics - Founder @ Reprogenetics (PGS/PGD
More informationPreimplantation genetic diagnosis
Preimplantation genetic diagnosis Borut Peterlin Clinical institute of medical genetics, University Medical Centre Ljubljana Outline of the presentation Primary prevention of genetic diseases Motivation
More informationAn Update on PGD: Where we are today
An Update on PGD: Where we are today Joyce Harper UCL Centre for PG&D and CRGH Institute for Womens Health University College London Overview What is PGD/PGS How we do it Disadvantages and advantages Future
More informationA Stepwise Approach to Embryo Selection and Implantation Success
Precise Genetic Carrier Screening An Overview A Stepwise Approach to Embryo Selection and Implantation Success Put today s most advanced genetic screening technology to work for you and your family s future.
More informationProblem Challenge Need. Solution Innovation Invention
Problem Challenge Need Solution Innovation Invention Tubal Infertility In-vitro Fertilisation Steptoe and Edwards Birth after the reimplantation of a human embryo. Lancet 1978 Louise Brown, 25. Juli 1978
More informationProf. Antonio Pellicer
Improving outcomes in ART : Time-lapse technology for monitoring COS and blastocyst culture Prof. Antonio Pellicer Instituto Valenciano de Infertilidad (IVI) University of Valencia apellicer@ivi.es www.ivi.es
More informationUvA-DARE (Digital Academic Repository) Preimplantation genetic screening: a reappraisal Mastenbroek, S. Link to publication
UvA-DARE (Digital Academic Repository) Preimplantation genetic screening: a reappraisal Mastenbroek, S. Link to publication Citation for published version (APA): Mastenbroek, S. (2011). Preimplantation
More informationPreimplantation Genetic Diagnosis (PGD) single gene disorders. A patient guide
Preimplantation Genetic Diagnosis (PGD) single gene disorders A patient guide Reproductive Genetic Innovations, LLC 2910 MacArthur Boulevard Northbrook, Illinois 60062 Phone: (847) 400-1515 Fax: (847)
More informationValidation of Next-Generation Sequencer for 24-Chromosome Aneuploidy Screening in Human Embryos
GENETIC TESTING AND MOLECULAR BIOMARKERS Volume 21, Number 11, 2017 ª Mary Ann Liebert, Inc. Pp. 1 7 DOI: 10.1089/gtmb.2017.0108 ORIGINAL ARTICLE Validation of Next-Generation Sequencer for 24-Chromosome
More informationPregnancy outcomes following 24-chromosome preimplantation genetic diagnosis in couples with balanced reciprocal or Robertsonian translocations
Pregnancy outcomes following 24-chromosome preimplantation genetic diagnosis in couples with balanced reciprocal or Robertsonian translocations Dennis Idowu, M.D., a Katrina Merrion, M.S., b Nina Wemmer,
More informationDr Devashana Gupta. Dr Karen Buckingham. Obstetrician and Gynaecologist Repromed. Repromed Auckland
Dr Karen Buckingham Obstetrician and Gynaecologist Repromed Dr Devashana Gupta Repromed Auckland 8:30-9:25 WS #93: Optimising Fertility - Periconception Management 9:35-10:30 WS #105: Optimising Fertility
More informationOrganisation of the PGD Centre. Overview. Setting up a PGD centre
Organisation of the PGD Centre Joyce Harper Chair of the ESHRE PGD Consortium Overview Setting up a PGD Centre Organisation of the PGD Centre Preparation for clinical PGD Misdiagnosis Accreditation External
More informationSelecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective
Thesis imedpub Journals http://www.imedpub.com Translational Biomedicine DOI: 10.21767/2172-0479.100002 Selecting a Single Embryo for Transfer after In-vitro Fertilization: A Translational Medicine Perspective
More information24sure TM Setting new standards in IVF
24sure TM Setting new standards in IVF 24sure TM The clinical challenge While in vitro fertilization (IVF) is a highly successful medical intervention that has revolutionized the treatment of infertility,
More informationMALBAC Technology and Its Application in Non-invasive Chromosome Screening (NICS)
MALBAC Technology and Its Application in Non-invasive Chromosome Screening (NICS) The Power of One Adapted from Internet Single Cell Genomic Studies Ultra Low Sample Input Advances and applications of
More informationDetection of aneuploidy in a single cell using the Ion ReproSeq PGS View Kit
APPLICATION NOTE Ion PGM System Detection of aneuploidy in a single cell using the Ion ReproSeq PGS View Kit Key findings The Ion PGM System, in concert with the Ion ReproSeq PGS View Kit and Ion Reporter
More informationCase Report What Drives Embryo Development? Chromosomal Normality or Mitochondria?
Hindawi Case Reports in Genetics Volume 2017, Article ID 4397434, 4 pages https://doi.org/10.1155/2017/4397434 Case Report What Drives Embryo Development? Chromosomal Normality or Mitochondria? A. Bayram,
More informationImplementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management
J Assist Reprod Genet (2016) 33:1279 1286 DOI 10.1007/s10815-016-0768-3 REVIEW Implementing PGD/PGD-A in IVF clinics: considerations for the best laboratory approach and management Antonio Capalbo 1,2
More informationSupplementary Appendix
Supplementary Appendix This appendix has been provided by the authors to give readers additional information about their work. Supplement to: Greco E, Minasi MG, Fiorentino F. Healthy babies after intrauterine
More informationHere s Looking at You, Kid: Time Lapse in the Clinical Embryology Laboratory
Here s Looking at You, Kid: Time Lapse in the Clinical Embryology Laboratory Thomas B. Pool, Ph.D., HCLD Fertility Center of San Antonio San Antonio, Texas Disclosures Speaker, Auxogyn Symposium, ASRM,
More informationDiagnosis of human preimplantation embryo viability
Human Reproduction Update, Vol.21, No.6 pp. 727 747, 2015 Advanced Access publication on January 6, 2015 doi:10.1093/humupd/dmu064 Diagnosis of human preimplantation embryo viability David K. Gardner 1,*,
More informationCHROMOSOME MICROARRAY TESTING (NON-ONCOLOGY CONDITIONS)
CHROMOSOME MICROARRAY TESTING (NON-ONCOLOGY CONDITIONS) UnitedHealthcare Oxford Clinical Policy Policy Number: LABORATORY 016.12 T2 Effective Date: June 1, 2018 Table of Contents Page INSTRUCTIONS FOR
More informationTelomeres and Genomic Instability In Preimplanation Embryos. David L. Keefe, M.D.
Telomeres and Genomic Instability In Preimplanation Embryos David L. Keefe, M.D. I. Global Genomic Instability, Including Aneuploidy, Mosaicism & Copy Number Variants, is Common In Preimplantation Embryos
More informationIndex. laboratory interventions emerging treatment options, freeze-all strategy, 67 vitrification, 68 morphologic features, 66
A Acquired thrombophilias, 128 130 Alcohol consumption, 44 American Fertility Society classification system, 162 American Society for Reproductive Medicine (ASRM), 34 Antiphospholipid antibody syndrome
More informationInfluence ovarian stimulation on oocyte and embryo quality. Prof.Dr. Bart CJM Fauser
Influence ovarian stimulation on oocyte and embryo quality Prof.Dr. Bart CJM Fauser How to balance too much vs too little? Lecture Outline Context ovarian stimulation Impact ovarian stimulation on oocyte
More informationAbstract. Introduction. RBMOnline - Vol 19. No Reproductive BioMedicine Online; on web 24 August 2009
RBMOnline - Vol 19. No 4. 2009 599 603 Reproductive BioMedicine Online; www.rbmonline.com/article/3872 on web 24 August 2009 Article Assisted reproduction in women over 40 years of age: how old is too
More information2017 Gap Analysis and Educational Needs Developed by the ASRM Continuing Medical Education Committee
TOPIC GAP(S) IDENTIFIED EDUCATIONAL NEED(S) Access to care Increase insurance coverage Strategies to increase coverage by selfinsureds and insurance companies. Alternatives to insurance coverage including
More informationPATIENT CONSENT FORM Preimplantation Genetic Screening (PGS) 24 Chromosome Aneuploidy and Translocation Screening with acgh
PREIMPLANTATION GENETIC SCREENING FOR ANEUPLOIDY SCREENING INTRODUCTION Preimplantation genetic screening (PGS) is used in conjunction with in-vitro fertilization (IVF) to screen embryos for numerical
More information