SUSTAINABLE&HEALTHY EFFOST, SITGES 14 NOV Agrifood and Bioscience Bioscience and Material. Research Institutes of Sweden
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1 SUSTAINABLE&HEALTHY EFFOST, SITGES 14 NOV 2017 Research Institutes of Sweden Agrifood and Bioscience Bioscience and Material
2 Objective Develop sustainable innovative technologies for manufacturing of sustainable, attractive bioactive ingredients and healthy food from agricultural by-products. 2
3 Driver for the project Berry processing industry generates a number of byproducts (e.g. press cakes/skins/seeds) Rich in bioactive compounds. Majority discarded as waste. 3
4 Project outline 4
5 Project partners RISE-Research Institutes of Sweden Warsaw University of Life Sciences Ruhr-Universität Bochum Technische Universität Berlin 5
6 Main findings - Extraction of bioactive compounds by pulsed electric fields (PEF) and supercritical fluid extraction (SFE) Drying and milling as pre-treatments Anthocyanin extraction yield could be improved by 40% by appropriate selection of drying technique and particle size. Freeze drying combined with particles < 710 μm was most successful PEF in a two-stage extraction process PEF as pre-treatment to high pressure CO2 extraction improved the anthocyanin yield when applied on unground fresh bilberry pomace, but not on heavily ground pomace 6 SFE of bilberry seed oil 200 bar and 60 C obtained higher recovery of vitamin E and higher antioxidant activity
7 Main findings -Extract stabilisation The high pressure process Particles from Gas Saturated Solutions (PGSS) and spray drying are used for the encapsulation of bilberry extract in particles of different shell materials. gas static mixer PGSS process extract + shell material filter Capsules of the bilberry extract and shell materials (Revel A, maltodextrin) have been successfully produced by PGSS. The particles were analyzed concerning particle size, bulk density, morphology and moisture content. spray tower dry powder dry powder 7 maltodextrin + bilberry Revel A + bilberry
8 Main findings -Extract stabilisation The high pressure process Particles from Gas Saturated Solutions (PGSS) and spray drying are used for the encapsulation of bilberry extract in particles of different shell materials. Capsules of the bilberry extract and the shell material Eudragit have been successfully produced by spray drying. The particles were analyzed concerning particle size, bulk density, morphology and moisture content. Eudragit + bilberry
9 Main findings - Added value emulsions Developed non-thermal emulsification method (o/w/o) where bilberry seed oil were located in the inner oil phase surrounded by an aqueous phase of anthocyanins stabilized by whey protein isolate. At ph 4.5 slow structure formation, rigid final product. Cause drops to agglomerate/align. At ph3 fast structure formation, soft final product. Anthocyanins has no notable effect on formation or resulting microstructure. Emulsifier generates smaller droplets 9
10 Main findings - Added value dried fruits Osmodehydrated fruit Bilberry by-products extract was used as a natural substance enriching in osmotic dehydration as a pretreatment. Osmo-dehydrated fruit were dried by freeze-drying and by convective-microwave-vacuum drying ( puffing ) Osmodehydrated in sucrose with 0, 5, 10, 15% extract and dried by puffing Osmotic dehydration for 24 h at 50 C generates high quality products, without shrinkage. Bilberry by-products extract or fruit concentrates are good as osmotic and natural enriching substances. Osmodehydrated in sucrose with 5, 10, 15% chokeberry concentrate and dried by puffing Attractive osmodehydrated dried fruit (snack) production both as puffing and freeze-drying method. Higher antioxidant activity of osmo-dehydrated products combined puffing drying in comparison to the freeze-drying. 10
11 Conclusions Selection of appropriate pre-treatments and extraction conditions were crucial to obtain an oil enriched in Vitamin E as well as to improve the anthocyanin extraction yield. Pressure and temperature influenced the recovery of Vitamin E and antioxidant activity of bilberry seed oil. PEF improved the anthocyanin extraction yield when applied on unground bilberry pomace. Bilberry extract was encapsulated in particles of different shell materials with the PGSS process and spray drying. It was possible to obtain different particle morphologies and sizes (5 to 60 µm) by controlling the processing parameters. The correlation between those processing parameters and resulting particle properties are valuable for different branches of the food industry for designing custom-made particles. 11
12 Conclusions Using non-thermal emulsification method it was possible to create stable micro capsules with anthocyanins stabilized by WPI in the continuous phase while encapsulating the bilberry seed oil. Final ph and emulsifier had significant impact on the structure of the micro capsules. Bilberry by-products extract may be used as enriching substance in osmotic dehydration pretreatment and also in sustanaible technology of dried fruit (pro-helthy snack with added value fruit) production. Puffing method generates high quality product, very similar to the freeze-dried fruit; the technique is also more economic (lower energy consumption); quick method (1-2 h) compared to freeze-drying (24 h). 12
13 Research Institutes of Sweden THANK YOU
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