Go beyond. to realities unexplored. Comprehensive workflows, integrated solutions
|
|
- Moris Greene
- 6 years ago
- Views:
Transcription
1 Go beyond to realities unexplored Comprehensive workflows, integrated solutions
2 We re committed to helping researchers and scientists in academia and industry harness the power of metabolomics to gain in-depth biological insight, facilitate biomarker discovery, and advance scientific research. By collaborating with key opinion leaders and others within the scientific community, we ve developed leading solutions in the areas of separation, detection, and software. And we ll continue to pursue advancements to delve deeper into the metabolome, so you can go beyond the edge of what we ever thought possible.
3 The total package Moving metabolomics forward requires an all-in commitment. That s why we ve invested heavily in developing new metabolomics software that lives up to the high standards set by our leading instrumentation. Now you can have the total package needed to perform increasingly complex analysis, and easily translate your metabolomics data into valuable discoveries for your next step.
4 A multitude of discoveries Driving advancements across a broad range of applications With its close link to phenotype, metabolomics is an ideal approach in areas of disease research to understand mechanisms of drug metabolism, biomarker discovery, and drug safety studies. Metabolomics approaches have also found wide applicability to other research areas such as the food and beverage industry, biofuels, and biochemicals. 4
5 We collaborate with leading metabolomics researchers across these industries to deliver results for diverse applications. Human health Metabolomics expands understanding in areas such as disease research, systems biology, and metabolic profiling, leading to insights into biomarker discovery and mechanisms for treatment, and driving new research directions. Plant science Metabolomics can help researchers understand the factors contributing to plant traits that influence yield, stress tolerance, seed quality, disease resistance, and mode of action of pesticides, knowledge of which will help to optimize crop development and production. Food science Through metabolomics, food scientists can rapidly obtain valuable information about the biochemical composition of foods. This helps in the derivation of components for food flavors and shelf life, leading to better control over food quality, among other factors. Nutrition and diet Using metabolomics, the biochemical profile of food components and its interaction with the biochemical networks of living organisms can be studied to provide information regarding the potential role of nutrition in health. This can help guide diet-based disease treatments and prevention. Drug development Metabolic profiling has the potential to identify markers arising from drug-induced toxicity leading to improvements in public safety and optimizing drug development. Environmental science Environmental metabolomics is an emerging approach to characterizing the effects of disease and toxic chemicals on sentinel species to understand their possible impact on humans. It is also a useful tool for carrying out chemical risk assessment of pharmaceuticals, pesticides, and other chemicals, and maintaining healthy livestock and fish. Biofuels and bioengineering The techniques of metabolomics can help researchers identify microbial strains and engineer novel metabolic routes for biomass production and conversion. This could potentially increase production of biofuels needed to meet future energy demands. 5
6 The core of successful metabolomics powered by Orbitrap mass spectrometry The success of metabolomics research lies with proven Thermo Scientific Orbitrap technology. Workflows that include Orbitrap mass spectrometers have already been widely adopted for life science applications such as proteomics. Based on published reports, the same technology is also suited for metabolomics. The Orbitrap mass spectrometers represent the leading mass spectrometry (MS) technology for analyzing samples that are complex both in the sheer number of analytes present and in the type of sample matrix, such as blood, food, urine, etc. Additionally, with a breadth of chromatographic separations and integrated data analysis tools, the Orbitrap mass spectrometer enables researchers to successfully analyze physicochemically diverse samples in metabolites making us the leader in metabolomics, delivering solutions to keep you at the forefront of science. 6
7 Comprehensive coverage of the metabolome Harnessing the power of chromatographic separation Since endogenous metabolites are diverse in their physicochemical properties and abundance, a true comprehensive metabolomics study will require multiple separation techniques. This complexity demands sophisticated methods of separation and detection high-resolution separation that is designed for high-resolution detection by Orbitrap mass spectrometers. The integration of each of the chromatographic separation methods (liquid, gas, and ion) with the high-resolution detection system (Orbitrap mass spectrometer) on one single platform enables broader, deeper, and faster analysis of complex and diverse metabolites offering a robust system that is more effective than the sum of its parts. Seamless integration with Orbitrap mass spectrometers Thermo Scientific products deliver comprehensive breadth in separation of analytes with gas, liquid, and ion chromatography systems. When combined with the leading Orbitrap mass spectrometer, our technologies enable broader and deeper analysis into the metabolome. With high robustness and technical reproducibility, these solutions provide novel yet stringent results for high-impact discoveries. LC-MS: Thermo Scientific Vanquish Flex Binary UHPLC system with Q Exactive HF MS GC-MS: Thermo Scientific Q Exactive GC Orbitrap GC-MS/MS system with proven TRACE 13 Series GC injectors IC-MS: Thermo Scientific Dionex ICS- 5+ Capillary HPIC System with Q Exactive HF MS 7
8 Comprehensive coverage of the metabolome Enabled by orthogonal separations and deep coverage with Orbitrap mass spectrometry Relative abundance 2 1 Peak #9, F6P Peak #1, G6P Retention time Separation of 11 monophosphate isomers of sugar by IC-MS. IC: Ion chromatography (IC)-MS is best suited for charged or very polar metabolites such as sugar phosphates and amino acids that are difficult to analyze by LC-MS. IC has high resolving power, and many isomers can be separated prior to mass spectrometry analysis by this technique. RT: Day Day 1 Relative abundance Day 2 Day Time (min) Quantitation of components in rat muscle homogenate was carried out by GC. Rat muscle tissues were decayed for 3 days (top to bottom). The peaks in the highlighted region indicate the change in intensity over time. GC: Samples that are volatile and amenable to chemical derivatization are well suited for gas chromatography (GC)-MS. GC offers high resolving power for separation of isomers. Plant secondary metabolites are well suited for this technique. 8
9 +ve and ve ion mode on the MS adds another dimension Fatty acid Galactose, nucleotide sugar Pentose and ascorbate Nucleotides Secondary metabolites RP-HPLC and ESI (+) HILIC and ESI (+) RP-HPLC and ESI ( ) HILIC and ESI ( ) RP- and NP-HPLC, and ESI (+/ ) Energy Amino acid GC and CI/EI IC and ESI ( ) Lipids Xenobiotics biodegradation Carbon fixation TCA Amino acid Amino acid Amino acid +ve and ve ion mode on the MS adds another dimension Ionic Ion chromatography Ionic property Liquid chromatography: HILIC Liquid chromatography: RP C18 Neutral Polar Polarity Nonpolar LC: Liquid chromatography (LC)-MS offers the broadest coverage of metabolites through the ability to change column chemistries, such as reversed-phase high-performance liquid chromatography (RP-HPLC) for nonpolar to moderately polar metabolites, and hydrophobic interaction liquid chromatography (HILIC) for ionic and polar compounds not retained by RP. Ultra high-performance LC (UHPLC) systems and columns are available for high-resolution separations. 9
10 Leading-edge metabolomics Powered by Orbitrap mass spectrometry When combined with advanced separation techniques, industry-leading Orbitrap system based mass spectrometers provide high-resolution accurate mass (HRAM) measurements and sensitivity required for metabolomics. This powerful technology enables high-throughput and quantitative capabilities, expanding the scope of our understanding of metabolites involved in cell metabolism and biological pathways, and in putative biomarker discovery. Key attributes of Orbitrap HRAM for successful metabolomics HRAM: With up to 5K resolution, Orbitrap analyzers enable more confident identification and quantitation due to high specificity allowing accurate mass assignments, especially with metabolites of similar mass, and isotope-labeling studies. The very high resolving power of Orbitrap HRAM analyzers resolves isobaric metabolites from a complex mixture in (A) plasma extract and (B) a standard mix of D-glucose and paraxanthine at concentrations of 5 mm and.1 mm, respectively. A B Relative abundance Relative abundance Relative abundance D-Glucose (m/z ) or Paraxanthine (m/z )? R = 6, R = 12, R = 24, R = 5, m/z m/z m/z m/z m/z m/z m/z m/z Peak intensity x1 6 5, 4, 3, 2, 1, A R 2 = pg 2 ng on column (1 fmol 1 pmol on column) 11 levels 5, 1, Amount (pg) 15, 2, Endogenous metabolites concentration range (mol/l) Peak intensity 1, 8, 6, 4, 2, B 3,89 pg n = 18 UM1 Citric acid 2,372 pg n = 24 UM2 7,779 pg n = 1 UM5 UM1 cells, very invasive UM2 cells, less invasive UM5 cells, highly invasive UM6 cells, less invasive 3,13 pg n = 12 UM6 Sensitivity: Metabolomics is and always has been a quantitative science. There are ordersof-magnitude differences between concentrations of different endogenous metabolites that must be detected for a comprehensive and meaningful view of their biological states. Orbitrap technology enables quantitation down to low-femtomole concentrations, with up to 5 orders of linear dynamic range with tight coefficients of variation (CV). 1 Sensitivity of HRAM analyzer: (A) Quantitation of citric acid was achieved at low-femtomole sensitivity using full scan MS. (B) shows the amount of endogenous citric acid obtained from each plate containing ~3 x 1 6 cells.
11 Resolution Accurate mass Technical reproducibility: Due to its sensitivity and selectivity, Orbitrap mass spectrometry techniques have become the method of choice for metabolomics research. To find meaningful answers with a large number of datasets, typically found with metabolomics, the mass spectrometer must provide accurate and reproducible data from run to run, without the need for internal calibration. 25 hr post-external calibration Relative abundance Mean Area 96,686, % CV 95,693,541 1.% 96,475,711.2% 95,147, % 98,394, % x Mean ppm ppm ppm ppm ppm Mean ppm ppm ppm ppm ppm 25 hr post-external calibration ,722, %.43 ppm ppm Time (min) m/z Relative Abundance scan points across a peak Full scan MS R = 7, Peak width = 3.6 s, mean 14.5 scans D3-hippuronic acid, theoretical m/z = Positive Relative abundance Time (min) Negative Fast polarity switching: A combination of separation techniques (GC, IC, and LC (RP or HILIC)) can help achieve a more comprehensive view of the metabolome. Additionally, more coverage can be obtained using both positive and negative ionization modes on the mass spectrometer. Polarity switching on an Orbitrap mass spectrometer can be achieved in a single run, enabling a more productive analysis. Base peak chromatograms of positive and negative ion modes of bovine heart lipid extract (1 µg on column) in a single run. Time (min) MS n capabilities for the structural elucidation of metabolites: Multiple-stage mass spectrometry (MS n ) offers a solution for de novo identification and structural elucidation. MS n is usually achieved using ion traps and Tribrid mass spectrometers such as the Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer. This is an advanced technique requiring high resolution, accurate mass (HRAM), and speed on an HPLC time scale, and a variety of dissociation modes such as high-energy collisional dissociation (HCD) and collision-induced dissociation (CID). Relative abundance Isoleucine Leucine sec HCD CID CID MS HCD 1 MS Orbitrap scan R = 12, CID CID 2 MS/MS HCD-ion trap scans 4 MS/MS Orbitrap scan R = 3, MS n improves the ability to confidently identify isomeric metabolites and elucidate structures of unknowns. 11
12 Numerous targets, unlimited possibilities Solutions designed to meet your metabolomics goals Several approaches to metabolomics research are available depending on the goals. The untargeted discovery approach can be used to identify putative biomarkers, understand metabolism on a system-wide scale, and detect unknown metabolites in screening applications. Targeted approaches are best suited when there is a focused study on a subset of known compounds, to verify and/or validate results from the discovery or hypothesis-driven phase, or for the routine detection and quantitation of compounds. We provide a range of solutions and workflows that are specifically designed to meet your needs Chromatographic separations Mass spectrometry Data analysis GC Untargeted metabolomics What is the global metabolite profile of a sample? HRAM detection by Orbitrap mass spectrometry Profiling Statistical analysis Identification Pathway analysis LC: HILIC Discovery phase or hypothesis driven LC: RP-HPLC (C-18) HRAM detection by Orbitrap mass spectrometry Profiling Quantitation Statistical analysis IC Targeted metabolomics What are the levels of specific metabolites in a sample? Selected reaction monitoring (SRM) by triple quadrupole mass spectrometry Quantitation Statistical analysis Reporting 12
13 Untargeted metabolomics Explore all metabolites and compounds present in many complex samples. Identify putative biomarkers Understand cellular metabolism Identify trends between samples and populations Study the most challenging samples and overcome the technical limitations associated with them Both the separation techniques and HRAM Orbitrap MS are proven to be highly reproducible, have high sensitivity, and are capable of measuring a wide range of metabolites, ranging from very hydrophilic to hydrophobic, for comprehensive coverage. Our metabolomics software is specially designed to examine the rich HRAM Orbitrap data to convert large datasets into meaningful insights. Targeted metabolomics Validate previously identified metabolites using a quantitative approach. Large-scale targeted profiling Target selected metabolites and compounds from either untargeted discovery or hypothesis-driven data Apply high-throughput and sensitive techniques Target and verify putative biomarkers or compounds indicative of quality, origin, and toxicity before developing routine assays Large-scale metabolomics quantitation using Orbitrap HRAM MS provides a workflow to quickly evaluate and screen known metabolites. As untargeted analyses are performed using HRAM Orbitrap systems, the same system can be used for targeted profiling with the same high selectivity, post-acquisition flexibility, and retrospective data analysis of compounds by MS/MS. Routine quantitation Target known, well-characterized metabolites and compounds Apply high-throughput and sensitive techniques Customize reports For targeted lists of known, well-characterized metabolites and compounds, the Thermo Scientific TSQ quadrupole mass spectrometers offer sensitive, fast, and robust quantitation of metabolites. 13
14 Tools for translational research Large-scale metabolic phenotyping As metabolism is closely associated with the dynamics of the human phenotype, metabolic phenotyping (phenome studies) can be applied to large-scale analysis of thousands of samples per study to identify a single metabolite or metabolite signatures that relate to disease onset and progression, or response to treatment. Several research initiatives are underway worldwide using this approach. SNP CNV LOH DNA methylation Histone modification TF binding Gene expression Alternative splicing Small RNA Protein expression PTM Cytokine array Metabolite profiling Metabolite flux Genome Epigenome Transcriptome Proteome Metabolome Phenome Transcription Expression Translation Function Aging Metabolicsyndrome Cardiovascular disease Cancer Due to its sensitivity, dynamic range, broad coverage, and high throughput, Orbitrap mass spectrometers are ideally suited to both small-scale metabolomics and large-scale metabolic phenotyping. The Phenome Centre Birmingham, UK, employs multiple Orbitrap LC-MS systems with the aim to complete more than 15, assays per year to study human metabolism and its role in health and disease. One such study applied a nontargeted discovery based metabolomics approach to investigate metabolic changes that relate to phenotypic changes, following burn trauma in an adult population. The study showed expected (e.g., fatty acid and carbohydrate metabolism) and unexpected (e.g., tryptophan metabolism) metabolic changes longitudinally, which may allow the development of improved nutritional support following trauma (A in figure). The study also identified putative prognostic markers of outcome, such as M3328 (e.g., later sepsis onset), which, following validation and replication, may be used to provide stratification and personalized treatment paths (B in figure). A A:D1-2 B 1, B:D3-4 C:D5-9 N6236 D12-15 D19-24 D26-29 G:M2 H:M2 I:M6 Triacylglycerides (TAG) Hypermetabolism is observed across many different metabolic classes in an adult sample population following burn trauma. Later onset of sepsis p =.72, FC = 5.2 M A:D B:D3-4 C:D5-9 N5131 D12-15 D19-24 D26-29 G:M2 H:M2 I:M6 Diacylglycerides (DAG) Time of sepsis p =.3, FC = 13.6 M A:D1-2 1, B:D3-4 C:D5-9 N274 D12-15 D19-24 D26-29 G:M2 H:M2 I:M6 Fatty acids Multiple organ failure p =.47, FC = 2.9 M3328 Data courtesy of the Phenome Centre Birmingham, UK. 14 Case Control Case A single metabolite shows promise as a biomarker for stratification, as it shows statistically significant changes before sepsis and during sepsis, and is also predictive of multiple organ failure. Control Case Control
15 Streamlined data analysis solutions Delivering the total package We have invested heavily in developing new metabolomics software that lives up to the high standards set by our leading instrumentation promising the same standards of quality, usability, and performance. Our suite of integrated applications is built to take you quickly from data acquisition to interactive analysis and interpretation of results. Customize your workflow with flexible architecture. Get simplified analysis of large-scale data. Now you can have the total package needed to perform increasingly complex analysis, and lead the way to high-impact discoveries. STATISTICAL ANALYSIS IDENTIFICATION Databases and libraries Untargeted metabolomics GC-MS Targeted quantitation with confirmation Targeted routine quantitation Raw data processing RELATIVE QUANTITATION ROUTINE QUANTITATION Compound Discoverer Software TARGETED PROFILING IDENTIFICATION TraceFinder Software PATHWAY ANALYSIS REPORT GENERATION Databases and libraries Targeted routine quantitation Results interpretation Biomarker discovery Dynamics of metabolism Metabolic alterations Biomarker verification and validation Verification of specific compounds and/or pathways of metabolism Flavor development and/or screening 15
16 Stringent and robust data analysis Compound Discoverer 2. Software From large datasets to pathway mapping Thermo Scientific Compound Discoverer Software is a flexible solution that lives up to the high standards of the Orbitrap mass spectrometer systems. Compound Discoverer Software offers a full suite of powerful tools for metabolomics analysis such as differential analysis, identification, and pathway mapping. It is the only analysis software that is designed to exploit the rich information of Orbitrap HRAM data. Compound Discoverer Software handles HRAM data from hundreds of samples, moving from data to biological interpretation. Powerful visualization tools and flexible workflows (standard and customizable) enable complete control of data processing. From the results obtained, customized reports are generated. STATISTICAL ANALYSIS IDENTIFICATION RELATIVE QUANTITATION Compound Discoverer Software PATHWAY ANALYSIS Detecting the components of interest in a sample is only the beginning. Compound Discoverer Software simplifies the next step determination of metabolite or compound structure by automatically annotating relevant components. This includes not only MS/MS analysis for the most stringent results, but also MS n analysis for structural elucidation of those compounds that are not confidently identified. MS n tree of prunin containing CID (white) and HCD spectra (green) with multiple collision energies from MS¹ down to the MS 5 level. Structure of prunin. 16
17 mzcloud online fragmentation library Powerful and rigorous compound identification Determining the components of interest in a complex analysis is only one piece of the metabolomics workflow. Compound identification remains one of the toughest challenges in many research applications. Compound Discoverer Software provides many tools to aid in compound identification, such as the unique, high-quality mzcloud online fragmentation library (mzcloud.org). Automatic identification of compounds using the mzcloud library without leaving Compound Discoverer Software. Map and Display features detect the potential metabolites of the biological pathways. mzcloud is a highly curated library, currently with >6, compounds with MS/MS fragmentation spectra. With major contributions from us and the metabolomics community, this number is increasing every day. Coupled with molecular weight or formula-based searches in the ChemSpider database and other user-created databases, Compound Discoverer Software gives you the power to turn unknowns into knowledge. MS 2 spectrum of prunin (HCD, 5 NCE) with annotated fragment structures. 17
18 Comprehensive portfolio Our complete Thermo Scientific instrument lineup One resource Thermo Fisher Scientific is a partner in the life sciences, providing innovative metabolomics solutions to address the challenges of biological research. We enable end-to-end workflows with column chemistries, comprehensive separation systems, mass spectrometry, and integrated software. For targeted routine analyses, the Thermo Scientific TSQ triple quadrupole mass spectrometer completes the lineup with leading HRAM Orbitrap MS systems. Dionex ICS-5+ Capillary HPIC System Vanquish Flex Binary UHPLC System Compound Discoverer Software Q Exactive HF Mass Spectrometer mzcloud online fragmentation library Orbitrap Fusion Lumos Mass Spectrometer der TraceFinder Software TSQ Quantiva Triple Quadrupole Mass Spectrometer Q Exactive GC Orbitrap Mass Spectrometer 18 TSQ 8 Evo Triple Quadrupole Mass Spectrometer
19
New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics
New Mass Spectrometry Tools to Transform Metabolomics and Lipidomics July.3.13 Ken Miller Vice President of Marketing, Life Sciences Mass Spectrometry 1 The world leader in serving science Omics & the
More informationNew Instruments and Services
New Instruments and Services Liwen Zhang Mass Spectrometry and Proteomics Facility The Ohio State University Summer Workshop 2016 Thermo Orbitrap Fusion http://planetorbitrap.com/orbitrap fusion Thermo
More informationJoin the mass movement towards mass spectrometry
Join the mass movement towards mass spectrometry Thermo Scientific ISQ EC single quadrupole mass spectrometer Embrace the power of mass spectrometry Achieving a comprehensive understanding of the samples
More informationDon t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry
Don t miss a thing on your peptide mapping journey How to get full coverage peptide maps using high resolution accurate mass spectrometry Kai Scheffler, PhD BioPharma Support Expert,LSMS Europe The world
More informationMASS SPECTROMETRY BASED METABOLOMICS. Pavel Aronov. ABRF2010 Metabolomics Research Group March 21, 2010
MASS SPECTROMETRY BASED METABOLOMICS Pavel Aronov ABRF2010 Metabolomics Research Group March 21, 2010 Types of Experiments in Metabolomics targeted non targeted Number of analyzed metabolites is limited
More informationMichal Godula Thermo Fisher Scientific. The world leader in serving science
Resolving the food authenticity challenges using advanced isotopic ratio and Thermo Scientific Orbitrap high resolution mass spectrometry tools in practice Michal Godula Thermo Fisher Scientific The world
More informationLC/MS/MS SOLUTIONS FOR LIPIDOMICS. Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS
LC/MS/MS SOLUTIONS FOR LIPIDOMICS Biomarker and Omics Solutions FOR DISCOVERY AND TARGETED LIPIDOMICS Lipids play a key role in many biological processes, such as the formation of cell membranes and signaling
More informationIncreased Identification Coverage and Throughput for Complex Lipidomes
Increased Identification Coverage and Throughput for Complex Lipidomes Reiko Kiyonami, David Peake, Yingying Huang, Thermo Fisher Scientific, San Jose, CA USA Application Note 607 Key Words Q Exactive
More informationTHE POWER OF MASS SPECTROMETRY FOR IC ANALYTICAL CHEMISTS. Unleash New Possibilities with Enhanced Detection for IC Applications
THE PWER F MA PECTRMETRY FR IC ANALYTICAL CHEMIT Unleash New Possibilities with Enhanced Detection for IC Applications THE PWER F MA PECTRMETRY Ion chromatography (IC) excels in analyzing ionic species
More informationChoosing the metabolomics platform
Choosing the metabolomics platform Stephen Barnes, PhD Department of Pharmacology & Toxicology University of Alabama at Birmingham sbarnes@uab.edu Challenges Unlike DNA, RNA and proteins, the metabolome
More informationNew Instruments and Services
New Instruments and Services http://planetorbitrap.com/orbitrap fusion Combining the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture, the Orbitrap Fusion
More informationOrbitrap ID-X Tribrid mass spectrometer. Transforming small molecule identification and characterization
rbitrap ID-X Tribrid mass spectrometer Transforming small molecule identification and characterization A leap ahead for small molecule analyses Acquisition and interpretation of mass spectra to characterize
More informationMASS SPECTROMETRY IN METABOLOMICS
For personal use only. Please do not reuse or reproduce without the author s permission MASS SPECTRMETRY IN METABLMICS Pavel Aronov Stanford Mass Spectrometry Users Meeting August 21, 2008 rigin of Metabolomics
More informationTerminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids
Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids Metabolome: the quantitative complement of all the low molecular weight molecules present in
More informationChallenges in Separation Technologies
Challenges in Separation Technologies Erin Shammel Baker Xing Zhang, Yehia Ibrahim, Matthew Monroe, Dennis Mehinagic, Justin Teeguarden, Thomas Metz and Richard D. Smith Pacific Northwest National Laboratory
More informationMetabolite identification in metabolomics: Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu utline Introduction Putative
More informationProfiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography
Profiling of Endogenous Metabolites Using Time-of-Flight LC/MS with Ion Pair Reverse Phase Chromatography Application Note Metabolomics Author Yuqin Dai Agilent Technologies, Inc. 31 Stevens Creek Blvd,
More informationRobust extraction, separation, and quantitation of structural isomer steroids from human plasma by SPE-UHPLC-MS/MS
TECHNICAL NOTE 21882 Robust extraction, separation, and quantitation of structural isomer steroids human plasma by SPE-UHPLC-MS/MS Authors Jon Bardsley 1, Kean Woodmansey 1, and Stacy Tremintin 2 1 Thermo
More informationUltra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification
22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and
More informationGlycerolipid Analysis. LC/MS/MS Analytical Services
Glycerolipid Analysis LC/MS/MS Analytical Services Molecular Characterization and Quantitation of Glycerophospholipids in Commercial Lecithins by High Performance Liquid Chromatography with Mass Spectrometric
More informationSupporting information
Supporting information A novel lipidomics workflow for improved human plasma identification and quantification using RPLC-MSn methods and isotope dilution strategies Evelyn Rampler 1,2,3, Angela Criscuolo
More informationImpact of Chromatography on Lipid Profiling of Liver Tissue Extracts
Impact of Chromatography on Lipid Profiling of Liver Tissue Extracts Application Note Clinical Research Authors Mark Sartain and Theodore Sana Agilent Technologies, Inc. Santa Clara, California, USA Introduction
More informationPTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System
Application Note LC/MS PTM Discovery Method for Automated Identification and Sequencing of Phosphopeptides Using the Q TRAP LC/MS/MS System Purpose This application note describes an automated workflow
More informationThermo Scientific LipidSearch Software for Lipidomics Workflows. Automated Identification and Relative. Quantitation of Lipids by LC/MS
Thermo Scientific LipidSearch Software for Lipidomics Workflows Automated Identification and Relative of Lipids by LC/MS The promise of lipidomics Lipidomics is a new field of study crucial for understanding
More informationMetabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra
Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu Outline Introduction
More informationMetabolomics: quantifying the phenotype
Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems
More informationNON TARGETED SEARCHING FOR FOOD
NON TARGETED SEARCHING FOR FOOD CONTAMINANTS USING ORBITRAP HIGH RESOLUTION MASS SPECTROMETRY Michal Godula 1, Adrian Charlton 2 and Klaus Mittendorf 1 1 Thermo Fisher Scientific, Dreieich, Germany 2 Food
More informationAchieve Broad Lipid Quantitation using a High-Throughput Targeted Lipidomics Method
Achieve Broad Lipid Quantitation using a High-Throughput Targeted Lipidomics Method LC-Based Approach for Lipid Class Separation and Quantitation on QTRAP 6500+ System Mackenzie Pearson, Santosh Kumar
More informationApplying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis
Applying a Novel Glycan Tagging Reagent, RapiFluor-MS, and an Integrated UPLC-FLR/QTof MS System for Low Abundant N-Glycan Analysis Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS
More informationLife Science and Chemical Analysis Solutions. Baljit Ubhi, Jeff Patrick, David Alonso, and Joe Shambaugh 1 2 3
Complementary LC- and GC- Mass Spectrometry Techniques Provide Broader Coverage of the Metabolome Cross-Platform Metabolomics Data Analysis Combining AB SCIEX LC/MS, LECO GC/MS, and Genedata Software 1
More informationO O H. Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL. LC /MS conditions
Simplifying Qual/Quan Analysis in Discovery DMPK using UPLC and Xevo TQ MS Robert S. Plumb and Paul D. Rainville Waters Corporation, Milford, MA, U.S. INTRODUCTION The determination of the drug metabolism
More informationSmall Molecule Science: Experimental designs for achieving ultra trace analysis
Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small
More informationSIEVE 2.1 Proteomics Example
SIEVE 2.1 Proteomics Example Software Overview What is SIEVE? SIEVE is Thermo Scientific s differential software solution. SIEVE will continue to enhance our current product for label-free differential
More informationCharacterization of an Unknown Compound Using the LTQ Orbitrap
Characterization of an Unknown Compound Using the LTQ rbitrap Donald Daley, Russell Scammell, Argenta Discovery Limited, 8/9 Spire Green Centre, Flex Meadow, Harlow, Essex, CM19 5TR, UK bjectives unknown
More informationMass Spectrometry based metabolomics
Mass Spectrometry based metabolomics Metabolomics- A realm of small molecules (
More informationAgilent 6410 Triple Quadrupole LC/MS. Sensitivity, Reliability, Value
Agilent 64 Triple Quadrupole LC/MS Sensitivity, Reliability, Value Sensitivity, Reliability, Value Whether you quantitate drug metabolites, measure herbicide levels in food, or determine contaminant levels
More informationBioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS
Bioanalytical Quantitation of Biotherapeutics Using Intact Protein vs. Proteolytic Peptides by LC-HR/AM on a Q Exactive MS Jenny Chen, Hongxia Wang, Zhiqi Hao, Patrick Bennett, and Greg Kilby Thermo Fisher
More informationMetabolomics Strategies
Metabolomics Strategies using GC-MS/MS-Technology Hans-Joachim Hübschmann GC/MS Technology Manager, Austin/Singapore Proteomics/Metabolomics Seminars March 2012 Thermo Scientific GC and GC-MS Product Line
More informationDiscovery Metabolomics - Quantitative Profiling of the Metabolome using TripleTOF Technology
ANSWERS FOR SCIENCE. KNOWLEDGE FOR LIFE. Discovery Metabolomics - Quantitative Profiling of the Metabolome using TripleTOF Technology Baljit Ubhi Ph.D ASMS Baltimore, June 2014 What is Metabolomics? Also
More informationAdvances in Hybrid Mass Spectrometry
The world leader in serving science Advances in Hybrid Mass Spectrometry ESAC 2008 Claire Dauly Field Marketing Specialist, Proteomics New hybrids instruments LTQ Orbitrap XL with ETD MALDI LTQ Orbitrap
More informationTerminology. Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids
Terminology Metabolite: substance produced or used during metabolism such as lipids, sugars and amino acids A metabolite may be described as a compound which is internalized, chemically converted or secreted
More informationSample Concentration and Analysis of Human Hormones in Drinking Water
Sample Concentration and Analysis of Human Hormones in Drinking Water Carl Fisher Applications Chemist Ion Chromatography/Sample Preparation Thermo Fisher Scientific March 1, 215 1 The world leader in
More informationThermo Fisher Scientific, Sunnyvale, CA, USA; 2 Thermo Fisher Scientific, San Jose, CA, USA
An Ultra High Resolution Glycan Column for Isomeric Separation and the Structural Identification of Labeled N-Glycans from Proteins Including Antibodies Udayanath Aich, 1 Julian Saba, 2 Rosa Viner, 2 Shanhua
More informationApplication Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform
Application Note # LCMS-89 High quantification efficiency in plasma targeted proteomics with a full-capability discovery Q-TOF platform Abstract Targeted proteomics for biomarker verification/validation
More informationMetabolomic fingerprinting of serum samples by direct infusion mass spectrometry
Metabolomic fingerprinting of serum samples by direct infusion mass spectrometry Raúl González-Domínguez * Department of Chemistry, Faculty of Experimental Sciences. University of Huelva, Spain. * Corresponding
More informationThe use of mass spectrometry in lipidomics. Outlines
The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid
More informationAmadeo R. Fernández-Alba
% of compounds % of compounds % of compounds % of compounds Amadeo R. Fernández-Alba LC-Orbitrap QExactive Focus Instrumental LOQ 1% 9% 8% 7% 6% 5% 4% 3% 2% 1% %.1 mg/g.2 mg/g Tomato.5 mg/g ddms2 Target
More informationProteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev
Proteomics of body liquids as a source for potential methods for medical diagnostics Prof. Dr. Evgeny Nikolaev Institute for Biochemical Physics, Rus. Acad. Sci., Moscow, Russia. Institute for Energy Problems
More informationTargeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS. Kayleigh Arthur
Targeted and untargeted metabolic profiling by incorporating scanning FAIMS into LC-MS Kayleigh Arthur K.Arthur@lboro.ac.uk Introduction LC-MS is a highly used technique for untargeted profiling analyses
More informationUltra High Definition Optimizing all Analytical Dimensions
Ultra High Definition Optimizing all Analytical Dimensions Sensitivity Dynamic Range Signal Response Linearity Separation Speed Peak Capacity Chromatogram Mass Spectrum Mass Accuracy Resolving Power Acquisition
More informationKey Advantages of Comprehensive Cannabis Analysis
Comprehensive Cannabis Analysis: Pesticides, Aflatoxins, Terpenes, and High Linear Dynamic Range Potency from One Extract Using One Column and One Solvent System Robert Di Lorenzo 1, Diana Tran 2, KC Hyland
More information[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector
Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA
More informationNIH Public Access Author Manuscript J Proteome Res. Author manuscript; available in PMC 2014 July 05.
NIH Public Access Author Manuscript Published in final edited form as: J Proteome Res. 2013 July 5; 12(7): 3071 3086. doi:10.1021/pr3011588. Evaluation and Optimization of Mass Spectrometric Settings during
More informationAbsoluteIDQ p180 Kit. Targeted Metabolite Identifi cation and Quantifi cation
AbsoluteIDQ p180 Kit Targeted Metabolite Identifi cation and Quantifi cation Bringing our targeted metabolomics expertise to your lab. The Biocrates AbsoluteIDQ p180 mass spectrometry Assay Preparation
More informationA Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids
A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids Jeremy Netto, 1 Stephen Wong, 1 Federico Torta, 2 Pradeep Narayanaswamy, 2
More informationThermo Scientific LCMS Solutions for Pesticide Analysis
Thermo Scientific LCMS Solutions for Pesticide Analysis Dipankar Ghosh, Ph.D Director, Environmental & Food Safety - LSMS San Jose, CA April 20, 2015 1 The world leader in serving science 2 Routine Testing
More informationSupporting information
Supporting information Figure legends Supplementary Table 1. Specific product ions obtained from fragmentation of lithium adducts in the positive ion mode comparing the different positional isomers of
More informationAbsoluteIDQ p150 Kit. Targeted Metabolite Identifi cation and Quantifi cation. Bringing our targeted metabolomics expertise to your lab.
AbsoluteIDQ p150 Kit Targeted Metabolite Identifi cation and Quantifi cation Bringing our targeted metabolomics expertise to your lab. The Biocrates AbsoluteIDQ p150 mass spectrometry Assay Preparation
More informationUPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes
UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F
More informationMetabolomic and Proteomics Solutions for Integrated Biology. Christine Miller Omics Market Manager ASMS 2015
Metabolomic and Proteomics Solutions for Integrated Biology Christine Miller Omics Market Manager ASMS 2015 Integrating Biological Analysis Using Pathways Protein A R HO R Protein B Protein X Identifies
More informationMALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH. December 19,
MALDI Imaging Drug Imaging Detlev Suckau Head of R&D MALDI Bruker Daltonik GmbH December 19, 2014 1 The principle of MALDI imaging Spatially resolved mass spectra are recorded Each mass signal represents
More informationFast and easy separation of 23 drugs of abuse. including high, stable resolution of isobaric opioids from human urine by UHPLC-MS/MS
TECHNICAL NOTE 21883 Fast and easy separation of 23 drugs of abuse including high, stable resolution of isobaric opioids from human urine by UHPLC-MS/MS Authors Kean Woodmansey 1, Jon Bardsley 1 and Stacy
More informationLOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016
LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry
More informationSYNAPT G2-S High Definition MS (HDMS) System
SYNAPT G2-S High Definition MS (HDMS) System High performance, versatility, and workflow efficiency of your MS system all play a crucial role in your ability to successfully reach your scientific and business
More informationMore structural information with MS n
PRODUCT SPECIFICATIONS The LTQ XL linear ion trap mass spectrometer More structural information with MS n The LTQ XL linear ion trap mass spectrometer delivers more structural information faster and with
More informationProfiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry
Profiling Flavonoid Isomers in Highly Complex Citrus Juice Samples Using UPLC Ion Mobility Time-of-Flight Mass Spectrometry Michael McCullagh, 1 Kieran Neeson, 2 and Antonietta Gledhill 1 Waters Corporation,
More informationRapid, Simple Impurity Characterization with the Xevo TQ Mass Spectrometer
Robert Plumb, Michael D. Jones, and Marian Twohig Waters Corporation, Milford, MA, USA INTRODUCTION The detection and characterization of impurities and degradation products of an active pharmaceutical
More informationAgilent s LC/MS Portfolio and Applications Examples
Agilent s LC/MS Portfolio and Applications Examples Sean Orlowicz LC/MS Product Specialist Agilent Technologies 31August Agilent s > 40 year heritage in mass spectrometry 1971, 5930A GC/MS 1971-2011 2005,
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Pratik Jagtap Managing Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s necessary for success using
More informationIncreasing Molecular Coverage in Complex Biological and Environmental Samples by Using IMS-MS
Increasing Molecular Coverage in Complex Biological and Environmental Samples by Using IMS-MS Erin Shammel Baker Kristin E. Burnum-Johnson, Jon M. Jacobs, Yehia M. Ibrahim, Daniel J. Orton, William F.
More informationResearch to Routine Workflows for Large and Small Molecules using the Q Exactive HR/MS
Research to Routine Workflows for Large and Small Molecules using the Q Exactive HR/MS Sept. 22, 2011 Patrick Bennett Director Pharma Strategic Marketing Thermo Fisher Scientific Agenda Definitions Research
More informationThe Agilent MassHunter Software. One Software for all Agilent Mass Spec Systems
The Agilent MassHunter Software One Software for all Agilent Mass Spec Systems MassHunter The most innovative MS SW! MassHunter Workstation One software for all Agilent MS Systems Minimize learning (and
More informationMoving from targeted towards non-targeted approaches
Gesundheitsdirektion Moving from targeted towards non-targeted approaches Anton Kaufmann Official Food Control Authority of the Canton of Zurich () Switzerland 2 Overview I From single residue to multi
More informationIdentification of Ginsenosides Using the SCIEX X500R QTOF System
Identification of Ginsenosides Using the SCIEX X500R QTOF System Wang Sha, Cheng Haiyan, Liu Ting, Li Lijun, Jin Wenhai[Author] SCIEX, Pacific Applications Support Center (Beijing). China Background Ginseng
More informationDesigner Fentanyls Drugs that kill and how to detect them. Cyclopropylfentanyl
Designer Fentanyls Drugs that kill and how to detect them Cyclopropylfentanyl Science for a safer world The in vitro metabolism of cyclopropylfentanyl Simon Hudson & Charlotte Cutler, Sport and Specialised
More informationThe Raptor HILIC-Si Column
The Raptor HILIC-Si Column With Raptor LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or core-shell particles) with the resolution
More informationHigh Throughput Workflow for Global Metabolomic Profiling of Biological Samples with RapidFire online SPE/MS system
High Throughput Workflow for Global Metabolomic Profiling of Biological Samples with RapidFire online SPE/MS system Michelle Romm, Ph.D. Applications Scientist, Agilent Technologies, Inc. 1 What is High
More informationAutomating Mass Spectrometry-Based Quantitative Glycomics using Tandem Mass Tag (TMT) Reagents with SimGlycan
PREMIER Biosoft Automating Mass Spectrometry-Based Quantitative Glycomics using Tandem Mass Tag (TMT) Reagents with SimGlycan Ne uaca2-3galb1-4glc NAcb1 6 Gal NAca -Thr 3 Ne uaca2-3galb1 Ningombam Sanjib
More informationSWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids
SWATH Acquisition Enables the Ultra-Fast and Accurate Determination of Novel Synthetic Opioids Data Independent Acquisition on TripleTOF and X-Series QTOF Systems for Seized Drug Analysis Oscar G. Cabrices
More informationCHARACTERIZATION AND DETECTION OF OLIVE OIL ADULTERATIONS USING CHEMOMETRICS
CHARACTERIZATION AND DETECTION OF OLIVE OIL ADULTERATIONS USING CHEMOMETRICS Paul Silcock and Diana Uria Waters Corporation, Manchester, UK AIM To demonstrate the use of UPLC -TOF to characterize and detect
More informationBenefits and Characteristic Applications of High Resolution GC/MS and LC/MS. Frank David RIC and Ghent University
Benefits and Characteristic Applications of High Resolution GC/MS and LC/MS. Frank David RIC and Ghent University Mass Spectrometry Structure Elucidation Selective and Sensitive Detection Identification
More informationHigh-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring Buprenorphine and Norbuprenorphine in Urine
High-Throughput, Cost-Efficient LC-MS/MS Forensic Method for Measuring and in Urine Xiaolei Xie, Joe DiBussolo, Marta Kozak; Thermo Fisher Scientific, San Jose, CA Application Note 627 Key Words, norbuprenorphine,
More informationThe Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides
The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides Mohammed Abrar Unilabs York Bioanalytical Solutions Outline Introduction Why LC-MS/MS? Limitations of
More informationComprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids
PO-CON1576E Comprehensive Two-Dimensional HPLC and Informative Data Processing for Pharmaceuticals and Lipids HPLC 2015 PSB-MULTI-06 Yoshiyuki WATABE, Tetsuo IIDA, Daisuke NAKAYAMA, Kanya TSUJII, Saki
More informationUse of RapidFire/MS for Metabolomics. - from untargeted to targeted and Pathway driven analysis. RapidFire/MS. Moritz Wagner Agilent Technologies
Use of RapidFire/MS for Metabolomics RapidFire/MS - from untargeted to targeted and Pathway driven analysis Moritz Wagner Agilent Technologies 1 Agilent RapidFire TM High-throughput Mass Spectrometry System
More informationSample Preparation is Key
PLOS ONE DOI: 10.1371/journal.pone.0117232 February 6, 2015 Presented by Katie Gibbs Sample Preparation is Key Sample extraction and instrumental analysis methods are well documented in metabolomics. Understanding
More informationChemical Analysis Business Operations Waters Corporation Milford MA
The Detection and Identification of Unknown Contaminants During ToF Screening and Structural Elucidation for Pesticides in River Water Using an Integrated Software Approach Chemical Analysis Business Operations
More informationfor new contaminants at ultra trace level by using high resolution mass spectrometry
Non-targeted screening for new contaminants at ultra trace level by using high resolution mass spectrometry Dr Igor Fochi LSMS Product Specialist Thermo Fisher Scientific What is Environmental Analysis
More informationLearning Objectives. Overview of topics to be discussed 10/25/2013 HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS
HIGH RESOLUTION MASS SPECTROMETRY (HRMS) IN DISCOVERY PROTEOMICS A clinical proteomics perspective Michael L. Merchant, PhD School of Medicine, University of Louisville Louisville, KY Learning Objectives
More information2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry
Dr. Sanjeeva Srivastava 1. Fundamental of Mass Spectrometry Role of MS and basic concepts 2. Ionization Sources 3. Mass Analyzers 4. Tandem Mass Spectrometry 2 1 MS basic concepts Mass spectrometry - technique
More informationAgilent Solutions for Lipidomics. Greater Insight into
Agilent Solutions for Lipidomics Greater Insight into Lipid Metabolism Understanding Lipidomics What is Lipidomics? The term "lipidome" refers to all the lipids that exist in an organism and their effects
More informationRapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition
Rapid Analysis of Water-Soluble Vitamins in Infant Formula by Standard-Addition Evelyn Goh Waters Pacific, Singapore APPLICATION BENEFITS This method allows for the simultaneous analysis of 12 water-soluble
More informationAnalytical Workflow for Extractable and Leachable Impurities
Analytical Workflow for Extractable and Leachable Impurities AAPS 2015 26 October 2015, Orlando FL Kyle D Silva The world leader in serving science WHAT are EXTRACTABLES and LEACHABLES? 2 3 What is E&L
More informationamazon speed Innovation with Integrity Ion Trap Performance Beyond Imagination Ion Trap MS
amazon speed Ion Trap Performance Beyond Imagination Innovation with Integrity Ion Trap MS amazon Turning Speed into Solutions The amazon speed ion trap series sets new analytical standards for proteomics
More informationAgilent 7700x ICP-MS
Agilent 7700x ICP-MS Metals analysis Mid ppt to low ppb detection range Option to detect in four different gas modes to gain optimum detection of multiple metals or interferences in a sample Autosampler
More informationSPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine
SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA
More informationIon Source. Mass Analyzer. Detector. intensity. mass/charge
Proteomics Informatics Overview of spectrometry (Week 2) Ion Source Analyzer Detector Peptide Fragmentation Ion Source Analyzer 1 Fragmentation Analyzer 2 Detector b y Liquid Chromatography (LC)-MS/MS
More informationQuantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System
Quantitative Analysis of Vit D Metabolites in Human Plasma using Exactive System Marta Kozak Clinical Research Applications Group Thermo Fisher Scientific San Jose CA Clinical Research use only, Not for
More informationLecture 3. Tandem MS & Protein Sequencing
Lecture 3 Tandem MS & Protein Sequencing Nancy Allbritton, M.D., Ph.D. Department of Physiology & Biophysics 824-9137 (office) nlallbri@uci.edu Office- Rm D349 Medical Science D Bldg. Tandem MS Steps:
More information