THE POWER OF MASS SPECTROMETRY FOR IC ANALYTICAL CHEMISTS. Unleash New Possibilities with Enhanced Detection for IC Applications

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1 THE PWER F MA PECTRMETRY FR IC ANALYTICAL CHEMIT Unleash New Possibilities with Enhanced Detection for IC Applications

2 THE PWER F MA PECTRMETRY Ion chromatography (IC) excels in analyzing ionic species analytes of major importance to environmental, food and beverage, pharmaceutical, life science, chemical, power generation, and electronics industries. As the ion chromatography innovator and technology leader for over 4 years, Thermo Fisher cientific understands what IC analytical chemists need for their work. There is a continuing need for today s researcher to detect small quantities of analytes in complex samples. But what if you need even lower levels of sensitivity and selectivity to add extra confidence to your results? Unleash New Possibilities with Enhanced Detection for IC Applications Combining ion chromatography (IC) with mass spectrometry (M) maximizes the power to detect unexpected coelutions of components and confirm trace components with the analytical confidence of mass spectrometry. Mass spectrometry dramatically improves your IC system capabilities and provides: Higher sensitivity and accurate quantitation Peak confirmation and evaluation of chromatographic peak purity Improved resolution of complex samples eamless integration of M data into your IC workflow

3 Coupling IC to M IC-M is a recommended method for sensitive and selective determinations of ions. Combining ion chromatography with M workflows increases efficiency and sensitivity, and improves resolution in complex samples, leading to more accurate quantitation, improved confidence, and more chemical information about every sample. Thermo Fisher cientific offers several different mass spectrometer options for IC systems: single quadrupole M triple quadrupole M high resolution-accurate mass (HRAM) hybrid quadrupole-rbitrap M As the capabilities of these instruments grow, so does their power to provide information about your compounds of interest. While a single quadrupole M adds sensitivity and peak confirmation to your analyses, a triple quadrupole M/M provides additional sensitivity, peak confirmation, and basic structural elucidation. HRAM rbitrap M adds advanced structural elucidation to IC-M analyses.

4 MRE CNFIDENCE Higher ensitivity and Accurate Quantitation For routine analyses where single digit parts-per-billion (ppb) sensitivities are required, a simple mass detector, such as a single quadrupole mass spectrometer, offers the required sensitivity. Mass detection adds confidence to your quantitation, enabling you to obtain limits of detection (LDs) at least an order of magnitude greater than those provided by conductivity detection. With the use of very specific M selected ion monitoring (IM) detection, ppb-level quantification can be performed with greater confidence on a wide range of real-world samples, such as bottled drinking water. tartrate External tartrate 14 counts*min Calibration curve of tartrate at concentrations from 2 ppb to 1 ppb, r 2 =.9998 Intensity (counts) 3, 8, 1.2e6 Chloride Phosphate.e Anions in a bottled water sample Nitrate Minutes ulfate IM = m/z 35.1 IM = m/z 62.1 IM = m/z 97.1

5 Peak Confirmation In addition to the enhanced detection, if your study requires peak confirmation or peak purity analysis the single quadrupole M detector such as the Thermo cientific IQ EC mass spectrometer will provide complementary molecular weight data, for an increased degree of confidence. 12 A 17 Counts-min AN151 conditions, IonPac A2 column, 1 µl injection, without desolvation solvent B IQ EC, IM Perchlorate, m/z Apex 11 perchlorate can: #222 RT:... % C Perchlorate 8 2 IQ EC, IM m/z 11 6 Counts-min Q u a n uppressed Conductivity µ-min Perchlorate? Min 16 m/z Determination of perchlorate in a Municipal Drinking Water ample. (A) The conductivity channel provides very limited sensitivity towards the perchlorate peak and no confirmatory data. (B) IM m/z 99 provides a very sensitive response towards perchlorate. (C) IM m/z 11 confirms the response as perchlorate by confirming the 3:1 ratio of m/z 99:11 expected for perchlorate. Where unknown chromatographic peaks appear, mass detection enables the IC analytical chemist to quickly and effectively suggest a number of possibilities for these unknown peaks. By scanning across a chromatographic peak with a mass detector, it is possible to detect the appearance of unusual masses.

6 FLEXIBLE IC-M CMBINATIN Thermo Fisher cientific offers two different IC systems, the Thermo cientific Dionex IC-6 HPIC system and the Thermo cientific Dionex Integrion HPIC system, suited for pairing with M detection. Each of these unique IC systems can be used with a variety of mass spectrometers, offering increasing levels of confidence, peak confirmation, and structural elucidation for your analyses. Dionex IC-6 HPIC system: A modular, highly flexible IC system designed to meet the widest range of applications. Dionex Integrion HPIC system A compact, integrated IC system for routine ion analysis demands, ease-of-use and reliability. Thermo cientific IQ EC ingle Quadrupole Mass pectrometer: An economical, benchtop mass detector designed for routine IC-M workflows.

7 Mass pectrometer Analyzer Mode of peration M M/M ensitivity IQ EC Quadrupole ingle ion monitoring (IM) TQ Quantis M Triple Quadrupole elected reaction monitoring (RM) TQ Altis M Triple Quadrupole elected reaction monitoring (RM) Q Exactive M rbitrap mass analyzer High Resolution Accurate Mass (HRAM) rbitrap Fusion M rbitrap mass analyzer High Resolution Accurate Mass (HRAM) Peak Confirmation tructural Elucidation Thermo cientific TQ Altis and Thermo cientific TQ Quantis Triple Quadrupole Mass pectrometers: Best-inclass benchtop M designed for quantitative performance, robustness, and value. Thermo cientific Q Exactive Hybrid Quadrupole-rbitrap Mass pectrometer: High-resolution, accuratemass (HRAM) benchtop M that delivers high resolution data for quantitative, screening and confirmation studies. Thermo cientific rbitrap Fusion Mass pectrometer: An instrument combining the best of quadrupole, ion trap and rbitrap mass analysis and ease of use for the highest performing research system particularly suited for life science applications.

8 RELVING CMPLEXITY Increased electivity for Complex amples When you need increased sensitivity and peak confirmation, the increased power of a triple quadrupole M may be the answer. The sensitivity and selectivity of M/M detection makes it a highly preferred method for complex sample matrices. For example, perchlorate in baby formula can be quantified, without enrichment at low parts-per-trillion (ppt) levels using simple sample preparation and a TQ Altis triple quadrupole mass spectrometer for detection. This system provides excellent reproducibility, accuracy, and precision. 1 6 Infant Formula 1 6 Milk m/z 99 ( 35 Cl 4 m/z 83 ( 35 Cl 3 m/z 99 ( 35 Cl 4 m/z 83 ( 35 Cl 3 Perchlorate by 99 Perchlorate by m/z 11 ( 37 Cl 4 m/z 85 ( 37 Cl 3 Perchlorate by 11 RT: AA: N: m/z 11 ( 37 Cl 4 m/z 85 ( 37 Cl 3 Perchlorate by m/z 17 ( 35 Cl 18 4 m/z 89 ( 35 Cl 18 3 m/z 17 ( 35 Cl 18 4 m/z 89 ( 35 Cl 18 3 Internal tandard Time (min) Internal tandard Time (min) Perchlorate in unspiked infant formula and milk samples. Calculated concentrations are in the 1 5 µg/l range.

9 electivity for Co-Eluting Peaks Combining ion chromatography with mass spectrometric detection offers great selectivity. In the example to the right, organic acids were analyzed by IC-M. Using M detection, each of these organic acids was observed as a single peak. By overlaying the individual responses for each recorded mass, it is possible to determine how the co-eluting organic acids contribute to the total chromatographic peak. ntetensity(counts) Response (µ) , 5 25, A tandard mix at.5 mg/l level Pyruvate IM 87 uccinate IM 117 3, Malate IM , RT: pyryvate RT: pyryvate - Area: RT: uccinate/malate RT: ucchate - Area: RT: Malate - Area: 42o18 RT: Citrate Citrate IM minute RT: Citrate - Area: B Response (µ) -5. 7, 18, Animal feed ample Citrate, m/z 191 Intetensity(counts) Lactate, m/z 89 3, 25, 7, Malate, m/z 133 Malonate, m/z 13 uccinate, m/z minute Determination of organic acids in Animal Feed by single quadrupole IC-M. (A) The conductivity channel provides sufficient response for the organic acids, but lacks resolution for malate, malonate and succinate. (B) The highly selective IM channels on the Thermo cientific IQ EC are able to respond individually to the three co-eluting organic acids, providing both quantitative and qualitative data.

10 tructural Elucidation Capabilities IC combined with high-resolution, accurate-mass (HRAM) rbitrap mass spectrometry is an ideal solution for component identification in non-targeted and unknown workflows. For example, identification of lithium-ion battery failure analysis products using HRAM M/M offers four-decimal-point accuracy to distinguish between species. The unique fragmentation signature enhances that confidence. In addition to validating a proposed degradation pathway, confidence in the results avoids spending research time and resources going in an unproductive direction. IC-M Analysis oftware Thermo cientific offers a range of software solutions to support IC-M applications. Thermo cientific Chromeleon Chromatography Data ystem (CD) software is ideal for routine quantitative workflows. Thermo cientific TraceFinder and/or Thermo cientific Xcalibur software is recommended for qualitative workflows (M-H) m/z P.3 ppm P.8 ppm C H 4 4 P.5 ppm H P ( C 3 H 8 4 P m/z ( ppm M/M C 3 H 4 4 P.5 ppm m/z HRAM M/M fragments facilitate identification of unknown compounds (M-H) C 3 H 7 4 m/z ( ppm m/z H ppm ppm H M/M C 3 H ppm C 3 H ppm m/z Find out more at thermofisher.com/icm 218 Thermo Fisher cientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher cientific and its subsidiaries unless otherwise specified. BR7288-EN 218