A novel inhibitor of plant pathogenic bacteria extracted from propolis residues
|
|
- Maud Simpson
- 5 years ago
- Views:
Transcription
1 A novel inhibitor of plant pathogenic bacteria extracted from propolis residues JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY YAN XU
2 1. Background 2.2 研究内容与方法 Ralstonia solanacearum Ralstonia solanacearum 2 nd Plant pathogenic bacteria in the world Ralstonia solanacearum (Smith) Bacterial wilt Distribution widest; Most harm Hardest to control; Most lost Direct economic losses $50,000,000,000 10
3 The prevention 2.2 研究内容与方法 of plant disease is a worldwide problem Control Traditional Biological Breeding for disease resistance More difficult, Long term A Avirulent strain Crop rotation cycles Geographic restrict, Difficult to promote Natural agent D B Biocontrol bacterium strain Chemical pesticide Toxic very much Pollute the environment C Induced resistance
4 1. Background 2.2 研究内容与方法 Natural source material Caffeic Acids Derivatives Caffeic acid and its ester derivatives are a kind of natural phenolic acids which are widely found in fruits, vegetables, cereals, and bee products, which have biological activities such as antioxidant, anti-inflammatory and scavenging free radicals. Name Abb. R- Caffeic acid CA -- Methyl caffeate MC CH 3 Ethyl caffeate EC CH 2 CH 3 Propyl caffeate PC CH 2 CH 2 CH3 Isopropyl caffeate IpC CH 2 CH 2 CH3 Rhodiola rosea L. Butyl caffeate BC (CH 2 ) 3 CH 3 Amyl caffeate AC (CH 2 ) 4 CH 3 Isoamyl caffeate IaC (CH 2 ) 4 CH 3 Colla Apis, Propolis Hexyl caffeate HexC (CH 2 ) 5 CH 3 Heptyl caffeate HepC (CH 2 ) 6 CH 3 Octyl caffeate OCC (CH 2 ) 7 CH 3 Caffeic acid phenethyl ester CAPE (C 6 H 6 )CH 2 CH 3
5 1. Background Propolis Colla Apis, Propolis 4,000 Y ago Alleviate inflammation by Greek 3,000 Y ago Formalined cadaver by Egyptians Widely application now
6 1. Backgound Propolis Residues Annual production: tons in China Active ingredient: phenolic acid, flavone, terpene Purple gold: antibacterial, antiviral, antioxidant Propolis Residues: tons per year Sforcin J M, Bankova V. Propolis: Is there a potential for the development of new drugs?[j]. Journal of Ethnopharmacology. 2011, 133(2): Cottica S M, Sabik H, Bélanger D, et al. Use of propolis extracts as antioxidant in dairy beverages enriched with conjugated linoleic acid[j]. European Food Research and Technology. 2015, 241(4):
7 2. Inhibitory activity Inhibition rate The inhibitory activity of the extract of propolis residues Chloroform extract(d) and Ethanol extract(f)>50% for 5 Races Ethanol extract(f): GIM1.74:99.73±0.13% RS-5:91.61±4.75% Fig.2.1 Bacterial grow inhibition (%) of various extracts from propolis residue against R. solanacearum strains including GMI1000, GIM1.76, GIM1.74, GIM1.71 and RS-5. Samples: CK, agricultural streptomycin, 0.30mg/mL (proposed dosage); A, B, C, D, E, F were 35% ethanol extract, methanol extract, ethanol extract, chloroform extract, ethyl acetate extract, petroleum ether extract from propolis residues, respectively, their concentrations were all 0.10 mg/ml.(%)
8 2. The antibacterial activities of propolis residues MIC MBC The inhibitory activity on Ralstonia solanacearum of propolis residues extracts Table 2.1 The MIC and MBC concentration (mg/ml) of R. solanacearum treated by 35% ethanol extract and ethanol extract from propolis residue Bacterial Strain 35% Ethanol Extract Ethanol Extract Race MIC MBC MIC MBC GMI GIM GIM GIM RS The antibacterial activity of wax propolis residues is mainly found in ethanol extract.
9 2. The antibacterial activities of propolis residues Swimming motility Pathogenicity Flagellum swimming Realize diffusion Pathogen transmission Fig.2.2 Swimming motility inhibition of the ethanol extract of propolis residue against R. solanacearum strains including GMI1000 (A), GIM1.76 (B), GIM1.74 (C), GIM1.71 (D) and RS-5 (E), respectively mg/ml ethanol extract of propolis residue against R. solanacearum 5 strains: good, RS-5 BEST
10 SEM Effect on the morphology of mulberry Ralstonia solanacearum treated with propolis residues extract Shapes vary in size Growth defect Rough surface Fig. 2.3 SEM images of R. solanacearum RS-5 untreated and treated with the 35% ethanol extract of propolis residues. (A) Untreated (ruler units: 2.0 μm); (B) CK-35% ethanol (ruler units: 500 nm); (C) Treated (ruler units: 2.0 μm); (D) Treated (ruler units: 1.0 μm). Growth inhibition and cell structure change
11 TEM Cell irregular sunken Cytoplasmic density not uniform Substance outflow, appears cavitation Permeability of cell membrane barrier Fig. 2.4 TEM images of R. solanacearum RS-5 untreated and treated with the 35% ethanol extract of propolis residues (A) Untreated; (B) 35% ethanol; (C) 0.20 mg/ml of 35% ethanol extract of propolis residues; (D) 0.20 mg/ml of 35% ethanol extract of propolis residues (ruler units: 0.2 μm).
12 3.Caffic acid and its ester in Propolis residues LC-MS Qualitative identification of caffeic acid derivatives in ethanol extract of propolis residues by LC-MS Fig. 2.5 LC-MS TIC spectra of the standards (A) and the ethanol extract of propolis residue (B) The peak time of CA, PC, BC, CAPE, AC is 0.34 min min min min and min
13 LC-MS CA, CAPE more Fig. 2.6 Mass spectra of caffic acid and its alkyl esters in the ethanol extract of propolis residues. Sample ion peaks: m/z Standard molecular weight [M-H] - : CA-179 PC-221 BC-235 CAPE-283 AC-249
14 LC-MS Table 2.2 Qualitative analysis of caffic acid and its alkyl esters in the propolis residue extract No. t R /min m/z 化合物归属 Chemical compound 化合物缩写 Chemicals abbreviation Caffeic acid CA Table 2.3 Distribution of caffic acid and its alkyl esters in the grading extracts of propolis residue Extracts of propolis residue 35% Ethanol extract CA PC BC CAPE AC Propyl caffeate PC Butyl caffeate BC Caffeic acid phenethyl ester CAPE Amyl caffeate AC Methanol extract Ethanol extract Chloroform extract Ethyl acetate extract Petroleum ether extract Freely soluble in polar solvents.
15 LC-MS/MS Quantitative analysis of main caffeic acid ingredients in propolis residues by LC-MS/MS Table 2.4 Contents of CA and CAPE from grading solvent extracts of propolis residue (mg/g) Extracts of propolis residue Batch CA Std Dev CAPE Std Dev 35% Ethanol extract Methanol extract Ethanol extract Chloroform extract Ethyl acetate extract Petroleum ether extract A B A B A B A B A B A B CA: 1.97mg/g, CAPE: 6.53mg/g Production place of origin OR Conditions of supercritical extraction process-difference
16 SUMMARY Propolis residue of different polar solvent extracts had antibacterial activity on 5 Races of Ralstonia solanacearum Ethanol extract is the best. Qualitative and quantitative analysis of the Caffic acid and its ester compound Based on anion full scanning and selective reaction monitoring mode : LC-MS LC-MS/MS It provides a new way for the development of the natural source of mulberry blight.
17 Thanks for your attention! JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY YAN XU
PROPOLIS AS A SOURCE OF NEW ANTI-PAENIBACILLUS LARVAE SUBSTANCES K. Bilikova 1, M. Popova 2, B, Trusheva 2, V. Bankova 2
PRPLIS AS A SURCE F NEW ANTI-PAENIBACILLUS LARVAE SUBSTANCES K. Bilikova 1, M. Popova 2, B, Trusheva 2, V. Bankova 2 1 Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia 2
More informationPlant Origin and Anti-bacterial Activity of Taiwanese Green Propolis
Plant Origin and Anti-bacterial Activity of Taiwanese Green Propolis Yue-Wen Chen 1, Show-Ru Yeh 1, James Kwei An 2, and Chia-Nan Chen 1 1 Dept. of Animal Science, National I-Lan University, Taiwan 2 Dept.
More informationLC-MS Analysis of Botanicals
Botanical workshop UAB, Sept 11, 26 LC-MS Analysis of Botanicals Jeevan K. Prasain, Ph.D. Department of Pharmacology & Toxicology, UAB Purdue-UAB Botanicals Center for Age-related Disease Applications
More informationBacterial Wilt Tolerance Improvement in Tomato. Dilip R. Panthee Department of Horticultural Science North Carolina State University
Bacterial Wilt Tolerance Improvement in Tomato Dilip R. Panthee Department of Horticultural Science North Carolina State University Bacterial Wilt of Tomato Ralstonia solanacearum is the soil-borne pathogen
More informationTaking inspiration from Nature: Biomimicry and Bioflavonoids from propolis
Taking inspiration from Nature: Biomimicry and Bioflavonoids from propolis Propolis is a mixture of substances secreted by plants in order to protect their buds from several exogenous factors like: weather
More informationTitle: Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L.
Author's response to reviews Title: Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. Authors: Naima Saeed (naima_qau@hotmail.com) Muhammad
More informationANALYSIS OF CARNAUBA WAX ON BPX5
FOO 01 - FOOD ANALYSIS OF CARNAUBA WAX ON BPX5 CARNAUBA WAX Column Part No.: 054118 Phase: BPX5, 0.25 µm film 12 m x 0.32 mm ID Initial Temp: 100 C, 2 min Rate: 10 C/min Final Temp: Detector: Sensitivity:
More informationCHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF FRENCH BFA PROPOLIS EXTRACTS
CHEMICAL CMPSITIN AND ANTIXIDANT ACTIVITY F FENCH BFA PPLIS EXTACTS Séverine BISAD PhD student (University of Angers, France) Supervisor : Pr. Pascal ICHMME CE Ballot-Flurin Apiculteurs (BFA) : Ms Catherine
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL Artepillin C, is it a good marker for quality control of Brazilian Green Propolis? Cui-ping Zhang 1, Xiao-ge Shen 1, Jia-wei Chen 1, Xia-sen Jiang 1, Kai Wang 2, Fu-liang Hu 1 *
More informationJoctorof PhiloSDpky in Jlgriculturt
BIOCHEMICAL AND BIOLOGICAL BASES OF RESISTANCE IN SOLANACEOUS VEGETABLES AGAINST. BACTERIAL WILT INCITED BY. RAlSTONIASOlANACEARUM(SMITH) VABUUCHI 1 Ai. By T. SHEELA PAUL THESIS Submitted in partial fulfilment
More informationExtraction of carotenoids from crustacean waste using organic solvents
Extraction of carotenoids from crustacean waste using organic solvents Hanif Houshmand Kouchi 1, Marzieh Moosavi-Nasab 2, Bahareh Shabanpour 1 1 Department of Fishery, Gorgan University of Agricultural
More informationGROWTH INHIBITION OF STRAIN H37 OF THE HUMAN TUBERCLE
GROWTH INHIBITION OF STRAIN H37 OF THE HUMAN TUBERCLE BACILLUS BY 4-N-ALKYLRESORCINOLS IN THE DEPTH OF A LIQUID, SYNTHETIC, NONPROTEIN CULTURE MEDIUM Colorado Foundation for Research in Tuberculosis at
More informationGC/MS BATCH NUMBER: C90106
GC/MS BATCH NUMBER: C90106 ESSENTIAL OIL: ROMAN CHAMOMILE BOTANICAL NAME: CHAMAEMELUM NOBILE ORIGIN: UK KEY CONSTITUENTS PRESENT IN THIS BATCH OF ROMAN CHAMOMILE OIL % 3-METHYLAMYL ANGELATE 17.8 METHALLYL
More informationANTIBACTERIAL ACTIVITY OF LEAF AND SEED EXTRACTS OF DELONIX REGIA AND ACHYRANTHUS ASPERA AGAINST SELECTED BACTERIAL STRAINS
Int. J. Pharm. Med. & Bio. Sc. 2013 Dhanalakshmi D and Manimegalai K, 2013 Research Paper ISSN 2278 5221 www.ijpmbs.com Vol. 2, No. 2, April 2013 2013 IJPMBS. All Rights Reserved ANTIBACTERIAL ACTIVITY
More informationIn Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata
Human Journals Research Article November 2016 Vol.:5, Issue:1 All rights are reserved by Darshan Singh et al. In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Keywords:
More informationIn vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [6]:28-34 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4
More informationEsters with Improved Hydroalcoholic Solubility. Posted February 15, 2008
Esters with Improved Hydroalcoholic Solubility Tony O Lenick Siltech LLC tolenick@mindspring.com Kevin O Lenick SurfaTech Corporation kevin@surfatech.com Posted February 15, 2008 www.surfatech.com Background
More informationAnalysis of several common. organic acids in tobacco leaf, snus, and moist snuff
Analysis of several common organic acids in tobacco leaf, snus, and moist snuff S. C. Moldoveanu, W. A. Scott R.J. Reynolds Tobacco Co. Background Among the organic acids commonly present in tobacco, are
More informationThe Leading edge of Oleo-Chemical Technology for the Agricultural Industry
The Leading edge of Oleo-Chemical Technology for the Agricultural Industry A material added to a tank mix to aid or modify the action of an agrichemical, or the physical characteristics of the mixture.
More informationExtraction Properties of Propolis with Ethanol Concentration
Original Article Journal of Apiculture (3) : 211~216 (2) Extraction Properties of Propolis with Ethanol Concentration Soon Ok Woo*, In-pyo Hong and Sang-mi Han Sericultural & Apicultural Materials Division,
More informationRapeseed pomace as a source of bioactives for health
Rapeseed pomace as a source of bioactives for health Dr. Giovanna Bermano Director, Centre for Obesity Research and Education (CORE) Co-Director, Centre of Natural Products for Health (CNP) Rapeseed Pomace
More informationideal chemical preservatives
Paper No.: 03 Paper Title: FOOD MICROBIOLOGY Module 12: Preservation of foods by chemical methods the ideal chemical preservatives CONTENTS 1. Introduction 2. An ideal antimicrobial preservative and added
More informationThe use of mass spectrometry in lipidomics. Outlines
The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid
More informationSelectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants
Selectivity Comparison of Agilent Poroshell 1 Phases in the Separation of Butter Antioxidants Application Note Food Testing & Agriculture Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd. Abstract
More informationAnalytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products)
Analytical Method for 2, 4, 5-T (Targeted to Agricultural, Animal and Fishery Products) The target compound to be determined is 2, 4, 5-T. 1. Instrument Liquid Chromatograph-tandem mass spectrometer (LC-MS/MS)
More information6. SUMMARY AND CONCLUSION
6. SUMMARY AND CONCLUSION Infectious diseases are one of man s oldest enemies. They continue to be a serious burden around the world, in developing and industrialised countries alike. It is said that every
More informationRapidly Analyze a Wide Range of Glycol Ethers by GC-MS Using the New Rxi -1301Sil MS Column
Rapidly Analyze a Wide Range of Glycol Ethers by GC-MS Using the New Rxi -101Sil MS Column By Rebecca Stevens and Chris English Abstract Chromatographic conditions were developed for a fast GC-MS glycol
More information6 DISCUSSION. Plants and plant products have been used medicinally for various. ailments for years. WHO has estimated that about 36,000 plant
168 6 DISCUSSION Plants and plant products have been used medicinally for various ailments for years. WHO has estimated that about 36,000 plant species are used worldwide for medical purposes out of the
More informationOBJECTIVE. Lipids are largely hydrocarbon derivatives and thus represent
Paper 4. Biomolecules and their interactions Module 20: Saturated and unsaturated fatty acids, Nomenclature of fatty acids and Essential and non-essential fatty acids OBJECTIVE The main aim of this module
More informationPreparation and characterization of Aloe vera extract
Chapter 2 Preparation and characterization of Aloe vera extract 2.1. INTRODUCTION Aloe vera ethanolic extract was prepared according to Noor et.al, 2008 with few modifications. The phytochemical analysis
More informationChapter 4 SUMMARY AND CONCLUSION
Chapter 4 SUMMARY AND CONCLUSION 203 Let food be thy medicine, thy medicine shall be thy food...hippocrates 204 4.1. The inverse association between intake of fruits and vegetables and delayed onset of
More informationInhibitory Effect of Different Solvent Extracts of Vitex negundo L. and Allium sativum L. on Phytopathogenic Bacteria
Available online at www.scholarsresearchlibrary.com Scholars Research Library Archives of Applied Science Research, 2011, 3 (1):1-8 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-508X CODEN
More informationInhibitory effect of different solvent extracts of Vitex negundo L. and Allium sativum L. on phytopathogenic bacteria
Available online at www.scholarsresearchlibrary.com Scholars Research Library Archives of Applied Science Research, 2010, 2 (6):325-331 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-508X
More informationTocopherols Proposed Annotation Change and Additional Listing
October 26, 2016 National Organic Standards Board USDA-AMS 1400 Independent Ave. SW Washington, D.C. 20250 Re: AMS-NOP-16-0049 Docket # AMS-NOP-16-0049 Dear National Organic Standards Board Members: The
More informationAntioxidant and Antimicrobial Activities of Tabebuia Rosea
24 sobiyana et al., Available Online http://www.ijncse.com ISSN Online: 2395-7018 2(6) (2015) 24-30 Antioxidant and Antimicrobial Activities of Tabebuia Rosea ABSTRACT P.Sobiyana *, G.Anburaj, R.Manikandan
More informationAntioxidant and Antimicrobial Activities of the Extracts of Sophora flavescens
Antioxidant and Antimicrobial Activities of the Extracts of Sophora flavescens Cheng-Hong Yang, Chi-Chun Wu, and Li-Yeh Chuang * Abstract The main goal of this study was to determine the antibacterial
More informationDaebong LS Co., Ltd. Nov. 2017
Daebong LS Co., Ltd. Nov. 2017 Jeju N Propolis Extrct AO(H) Active solution for microbial for oral care & wound healing for skin care CONTENTS 1 2 3 4 KEY POINT GENERAL INFORMATION JEJU PROPOLIS SPECIFICATION
More informationFizzy Extraction of Volatile and Semivolatile Compounds into the Gas Phase
SUPPORTING INFORMATION Fizzy Extraction of Volatile and Semivolatile Compounds into the Gas Phase Cheng-Hao Chang, Pawel L. Urban* Department of Applied Chemistry, National Chiao Tung University 1001 University
More informationMolecules 2018, 23, 2472; doi: /molecules
Supplementary Materials: Polyphenol-Rich Extracts from Cotoneaster Leaves Inhibit Pro-Inflammatory Enzymes and Protect Human Plasma Components Against Oxidative Stress in vitro Agnieszka Kicel, Joanna
More informationContents. 01 Welded Syringe Filter Economy Syringe Filter Sterile Syringe Filter Membrane Filter 21
C0000613 C0000613 Contents LABFIL is a product brand under ALWSCI Technologies, which provides wide selection of syringe f ilter and membranes. It is our great pleasure to have customer be satisf ied with
More informationTenofovir disoproxil fumarate (Tenofoviri disoproxili fumaras)
C 19 H 30 N 5 O 10 P. C 4 H 4 O 4 Relative molecular mass. 635.5. Chemical names. bis(1-methylethyl) 5-{[(1R)-2-(6-amino-9H-purin-9-yl)-1-methylethoxy]methyl}-5-oxo-2,4,6,8-tetraoxa-5-λ 5 - phosphanonanedioate
More informationEUDRAGIT E 100, EUDRAGIT E PO and
Technical Information EUDRAGIT E 100, and Specification and Test Methods Ph. Eur. USP/NF JPE Basic Butylated Methacrylate Copolymer Amino Methacrylate Copolymer - NF Aminoalkyl Methacrylate Copolymer E
More informationAntimicrobial Investigation and determination of total phenolic and flavonoid contents of Indian Propolis from Satpuda Hills of Maharashtra
Malaya Journal of Biosciences 2014, 1(4):226-230 ISSN 2348-6236 print /2348-3075 online Malaya Journal of Biosciences RESEARCH ARTICLE www.malayabiosciences.com Open Access Full Text Article Antimicrobial
More information[PHAR06] Anti Helicobacter pylori from extracts of ten species of Phyllanthus sp. with special emphasis on chloroform extracts of Phyllanthus pulcher
The th Annual Seminar of National Science Fellowship [PHAR] Anti Helicobacter pylori from extracts of ten species of Phyllanthus sp. with special emphasis on chloroform extracts of Phyllanthus pulcher
More informationEUDRAGIT L 100 and EUDRAGIT S 100
Technical Information EUDRAGIT L 100 and EUDRAGIT S 100 Specification and Test Methods Ph. Eur. Methacrylic Acid - Methyl Methacrylate Copolymer (1:1) Methacrylic Acid - Methyl Methacrylate Copolymer (1:2)
More informationChapter 3 Preliminary extraction studies
Chapter 3 Preliminary extraction studies 3.1 Introduction The main aim of preliminary extraction studies was to identify the solvents that extracted the largest quantities of material while also extracting
More informationCHAPTER-6 IDENTIFICATION, AND CHARACTERISATION OF DEGRADATION IMPURITY IN VALSARTAN TABLETS
129 CHAPTER-6 IDENTIFICATION, AND CHARACTERISATION OF DEGRADATION IMPURITY IN VALSARTAN TABLETS 130 6.1. Introduction Valsartan is an orally active specific angiotensin II blocker effective in lowering
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(12):519-523 Research Article ISSN : 0975-7384 CDEN(USA) : JCPRC5 Characterization of cyanidin 3-(6-acetylglucoside)-5-(3
More informationSupercritical Carbon Dioxide Extraction Enhances Flavonoids in Water-Soluble Propolis
J. Chin. Inst. Chem. Engrs., Vol. 33, No. 3, 233-241, 2002 Supercritical Carbon Dioxide Extraction Enhances Flavonoids in Water-Soluble Propolis Geng-Shian You [1], Sun-Che Lin [2], Chao-Ruey Chen [3],
More informationApplicationNOTE EXACT MASS MEASUREMENT OF ACTIVE COMPONENTS OF TRADITIONAL HERBAL MEDICINES BY ORTHOGONAL ACCELERATION TIME-OF-FLIGHT.
Objective The aim of the study presented is to determine the presence of target Gingkolides in Gingko biloba leaf extract. The data presented shows Gingkolides determined to be present in the extract analyzed.
More informationAliphatic acids. Cycloaliphatic compounds. Lactone carboxylic acids. Polyketide pathway LICHEN. Shikimic acid. pathway
LICHEN CHEMISTRY The secondary chemicals produced by lichens have attracted the attention of investigators for over 100 years, and information about their structure, biogenic origin and phylogenetic significance
More information*MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN. Department of Botany, GC University, Lahore. ABSTRACT INTRODUCTION
BIOLOGIA (PAKISTAN) 01, 8 (1&), 17-17 PK ISSN 000-09 Antibacterial activity of crude s of different parts of Butea monosperma (Lamk.) Taub. *MIAN SHAHZADA ZIA AHMAD & ZAHEER-UD-DIN KHAN Department of Botany,
More informationThe four levels of protein structure are: primary structure, secondary structure, tertiary structure, and quaternary structure.
Proteins Proteins are organic complex nitrogenous compounds of high molecular weight, formed of C, H, O and N. They are formed of a number of amino acids linked together by peptide linkage [-CO-NH-]. Proteins
More informationOrganic Chemistry Part 2
Organic Chemistry Part 2 Benzene Benzene is a special structure C 6 H 6 The carbon-carbon bonds aren t a single or double bond but something in-between Resonance bond CYCLIC HYDROCARBONS Carbon chains
More informationWORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor 6.041 Volume 5, Issue 6, 1884-1891 Research Article ISSN 2278 4357 DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID
More informationChemical Compatibility Guide for: Ammex Gloves
Chemical Compatibility Guide for: Ammex Gloves NOTICE: The guide on the following page(s) was provided by the supplier. New Pig Corporation assumes no responsibility, obligation, or liability in conjunction
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationSupporting Information for
Supporting Information for Tandem Mass Spectrometry Assays of Palmitoyl Protein Thioesterase and Tripeptidyl Peptidase Activity in Dried Blood Spots for the Detection of Neuronal Ceroid Lipofuscinoses
More informationFINISHED PRODUCT SPECIFICATION
Product : Triptorelin Product / Item No : 48005 Specification : PPLI/FPS/TP01-1.1 Synonym : Not Applicable Abbreviation : Not Applicable Molecular weight : 1311.47 Effective Date : APPROVALS Prepared by
More informationPolymer Additive Analysis by EI and APCI
Polymer Additive Analysis by EI and APCI Kate Yu, Eric Block LC/MS Waters Corporation Introduction to polymer additive analysis by LC/MS. Electron Ionization (EI) Advantages Classical spectra Library searchable
More information4. Chanda, S. et al. (2012) evaluated the antioxidant potential of Manilkara zapota (L.)
Review of Literature: 1. Seshadri et al. (2010) screened the methanolic and acetone extracts of Manilkara zapota (L.) seeds for antibacterial activity along with two other plant seeds against various gram
More informationControlled Extraction Study Unknown Elastomer
Controlled Extraction Study Unknown Elastomer Dr. Alice Granger, Keith McKellop, Dr. Daniel L. Norwood IPAC-RS (Boehringer Ingelheim Pharmaceuticals, Inc.) Chemistry: Phase 1 Studies Controlled Extraction
More informationSPE Cartridges. All Finneran Products Are Certified. Conditioning Sample application Wash Elution
All Finneran Products Are Certified Finneran Solid Phase Extraction (SPE) cartridges provide a fast and efficient sample clean-up and concentration prior to analysis through GC, HPLC, and/or other instrument
More informationSummary Chapter 8 CHAPTER 8. Summary. Page 173
CHAPTER 8 Summary Page 173 Chapter 2: Liquid Chromatography/Tandem Mass Spectrometry Method for Quantitative Estimation of PEG 400 and its Applications A rapid sensitive and selective MRM based method
More informationPRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA CORCHORIFOLIA PLANT EXTRACTS
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article PRELIMINARY PHYTOCHEMICAL SCREENING AND IN-VITRO FREE RADICAL SCAVENGING ACTIVITY OF MELOCHIA
More informationJl. Perintis Kemerdekaan KM.5 Makassar 90231, South Sulawesi Indonesia.
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com ANTIMICROBIAL POTENCY OF PASSIFLORA FOETIDA LINN FROM SOUTH SULAWESI INDONESIA AGAINST BACTERIA IN VITRO Andi
More informationOpportunities with Grains for Health: Breeding and Production
Opportunities with Grains for Health: Breeding and Production Dr. Nancy Ames Agriculture and Agri-Food Canada Richardson Centre for Functional Foods and Nutraceuticals Winnipeg, Manitoba, Canada June 25,
More informationAnalytical Challenges in Veterinary Toxicology: Bromethalin
Analytical Challenges in Veterinary Toxicology: Bromethalin Mike Filigenzi, Birgit Puschner, Linda Aston, Robert Poppenga CAHFS-UC Davis School of Veterinary Medicine First things first: Acknowledgements
More information6/9/2015. Unit 15: Organic Chemistry Lesson 15.2: Substituted Hydrocarbons & Functional Groups
1-chloropropane 2-methylpropane 1-iodobutane Ethanoic Acid Unit 15: Organic Chemistry Lesson 15.2: Substituted Hydrocarbons & Functional Groups 43 It Ain t Just Hydrocarbons There are all sorts of organic
More informationSeaweed based antioxidants analysis and application
Seaweed based antioxidants analysis and application Ditte B Hermund, PhD student Rósa Jónsdóttir and Hordur G Kristinsson, Matís, Iceland Charlotte Jacobsen and Kristian F Nielsen, DTU, Denmark Background
More informationGC/MS BATCH NUMBER: J10101
GC/MS BATCH NUMBER: J10101 ESSENTIAL OIL: JASMINE ABSOLUTE BOTANICAL NAME: JASMINUM SAMBAC ORIGIN: EGYPT KEY CONSTITUENTS PRESENT IN THIS BATCH OF JASMINE ABSOLUTE OIL (E,E)-α-FARNESENE 13.9 LINALOOL 9.5
More informationFast determination of residual glycerol and glycerides in biodiesel by SFC/MS using the Agilent 1260 Infinity Analytical SFC System
Fast deteration of residual glycerol and glycerides in biodiesel by SFC/MS using the Agilent 126 Infinity Analytical SFC System Application Note Petrochemical Authors Maria Rambla Alegre, Melissa N. Dunkle,
More informationAntiradical activity and polyphenol content of ethanolic extracts of Propolis
RESEARCH PAPER OPEN ACCESS Antiradical activity and polyphenol content of ethanolic extracts of Propolis Njintang Yanou Nicolas 1*, Tatsadjieu Ngoune Leopold 2, Ngakou Albert 1, Danra Dourandi 1, Tchuenguem-Fohouo
More informationAnalysis of Antioxidants in Vegetable Oils Using the Agilent 1260 Infinity Hybrid SFC/UHPLC System with MS Detection
Analysis of Antioxidants in Vegetable Oils Using the Agilent Infinity Hybrid SFC/UHPLC System with MS Detection Application Note Food Analysis & Agriculture Authors Maria Rambla-Alegre, Melissa N. Dunkle,
More informationScreening of Active Components as Scavenger of Radical from Perennial Fujimoto Bean (Hyacinth Bean) Whole Herb by HPLC MS Coupled Radical Reaction
Original Research Paper Acta Chromatographica 28(2016)1, 19 31 DOI: 10.1556/1326.2016.28.1.02 Screening of Active Components as Scavenger of Radical from Perennial Fujimoto Bean (Hyacinth Bean) Whole Herb
More informationTank Armor SERIES 390. Chemical Resistance. 1, 1, 1-Trichloroethane (Trichloroethane) FC, OC. Aluminum Sulfate (49%) IS, TS, SC, FC,
Tank Armor SERIES 390 (IS) - Immersion Service (TS) - Temporary Storage (SC) - Secondary Containment (FC) - Frequent Contact () - Occasional Contact (NR) - Not Recommended (NT) - Not Tested (PC) - Please
More informationIDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF METHANOL
Open Access Research Journal www.pradec.eu Medical and Health Science Journal, MHSJ ISSN: 1804-1884 (Print) 1805-5014 (Online) Volume 12, 2012, pp.70-77 IDENTIFICATION AND ANTIBACTERIAL ACTIVITY OF METHANOL
More informationMatrix-induced Signal Enhancement of Propamocarb in LC-MS/MS
Observations concerning a compound a matrix a method other Matrix-induced Signal Enhancement of Propamocarb in LC-MS/MS Reported by: EURL-SRM Version 1 (last update: 24.10.2012) Brief description of problem/observation/solution:
More informationGC/MS BATCH NUMBER: J10102
GC/MS BATCH NUMBER: J10102 ESSENTIAL OIL: JASMINE ABSOLUTE BOTANICAL NAME: JASMINUM SAMBAC ORIGIN: INDIA KEY CONSTITUENTS PRESENT IN THIS BATCH OF JASMINE ABSOLUTE OIL % (E,E)-α-FARNESENE 8.3 δ-cadinene
More informationMechanochemical Modification of Lignin and Application of the Modified Lignin for Polymer Materials
Mechanochemical Modification of Lignin and Application of the Modified Lignin for Polymer Materials Jinwen Zhang Composite Materials and Engineering Center Washington State University Significance Petroleum-based
More informationSpecific N-Alkylation of Hydroxypyridines Achieved by a Catalyst- and Base-Free Reaction with Organohalides
Supporting Information Specific N-Alkylation of Hydroxypyridines Achieved by a Catalyst- and Base-Free Reaction with Organohalides Bin Feng, Yang Li, Huan Li, Xu Zhang, Huamei Xie, Hongen Cao, Lei Yu,
More informationAnalytical and Preparative SFC Columns
Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...
More informationSecrets to Successful GC-MS/MS Pesticide Method Development
Secrets to Successful GC-MS/MS Pesticide Method Development Katerina Mastovska Nutritional Chemistry and Food Safety Covance Laboratories QuEChERS vs. Traditional Methods Quick, Easy, Cheap, Effective,
More informationApplication Note: 425. Introduction. Goal. Experimental Conditions
Application Note: 425 Determination of Different Classes of Pesticide Residues in Processed Fruits and Vegetables by LC-MS Using the TSQ Quantum Ultra According to EU Directive 91/414 EEC Eleni Botitsi,
More informationEvaluation of a New Ionic Liquid Stationary Phase with PEG Like Selectivity
Evaluation of a New Ionic Liquid Stationary Phase with PEG Like Selectivity L.M.Sidisky, G. A. Baney, J.L. Desorcie, D.L. Shollenberger, G. Serrano, and K.K. Stenerson Supelco, Div. of Sigma-Aldrich Bellefonte,
More informationZillillah, a Guowei Tan, a,b and Zhi Li* a,b. 4 Engineering Drive 4, Singapore Fax: ; Tel:
Highly Active, Stable, and Recyclable Magnetic Nano-size Solid Acid Catalysts: Efficient Esterification of Free Fatty Acid in Grease to Produce Biodiesel Zillillah, a Guowei Tan, a,b and Zhi Li* a,b a
More informationSupporting Information. Effect-directed discovery of bioactive compounds followed by. highly targeted characterization, isolation and identification,
Supporting Information Effect-directed discovery of bioactive compounds followed by highly targeted characterization, isolation and identification, exemplarily shown for Solidago virgaurea Ágnes M. Móricz
More informationSuggested homework problems: 8.8, 8.13, ,
Chapter 8 Outline: Haloalakanes, Halogenation, & Radical reactions Cover 8.1-8.3 on your own 1. Halogenation of Alkanes A. Mechanism of Halogenation B. Regioselectivity & Stereoselectivity of Halogenation
More informationNew GCMS Applications
New GCMS Applications Analysis of Trace Fatty Acid Methyl Esters (FAME) in Jet Fuel, Sample Characterization by GCxGC/FID/MSD, Crude Oil Biomarkers Malgorzata Sierocinska Agilent Technologies Waldbronn
More informationIn vitro study of antibacterial activity of Carissa carandas leaf extracts
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (1):36-40 ISSN : 2249-7412 CODEN (USA): AJPSKY In vitro study of antibacterial activity of Carissa
More informationChapter-8 Conclusion and Future Scope of the Study
Chapter-8 Conclusion and Future Scope of the Study 8.1. Conclusion The present investigation was carried under five clearly focused objectives. Different varieties of Amla were evaluated for their nutritional
More informationIdentification of Imidacloprid Metabolites in Onions Using High Resolution Accurate Mass Spectrometry (LC/Q-TOF MS) and Accurate Mass Tools
Identification of Imidacloprid Metabolites in Onions Using High Resolution Accurate Mass Spectrometry (LC/Q-TOF MS) and Accurate Mass Tools Application ote Food Authors E. Michael Thurman and Imma Ferrer
More informationQuantitative Determination of Free Radical Scavenging, Antitumor and Antimicrobial Activities of Some Myanmar Herbal Plants
2011 International Conference on Biomedical Engineering and Technology IPCBEE vol.11 (2011) (2011) IACSIT Press, Singapore Quantitative Determination of Free Radical Scavenging, Antitumor and Antimicrobial
More informationSubcritical Water Extraction of Polyphenolic Compounds from Terminalia chebula Fruits
Chiang Mai J. Sci. 2008; 35(1) FU-003 103 Chiang Mai J. Sci. 2008; 35(1) : 103-108 www.science.cmu.ac.th/journal-science/josci.html Contributed Paper Subcritical Water Extraction of Polyphenolic Compounds
More informationSeparation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement
Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement Application Note Food Testing & Agriculture Author Edgar Naegele Agilent Technologies,
More informationFundamentals of Organic Chemistry CHEM 109 For Students of Health Colleges Credit hrs.: (2+1)
Fundamentals of Organic Chemistry CHEM 109 For Students of Health Colleges Credit hrs.: (2+1) King Saud University College of Science, Chemistry Department CHEM 109 CHAPTER 7. CARBOXYLIC ACIDS AND THEIR
More informationRAPID CITY, PIERRE & EASTERN RAILROAD RCPE TARIFF 4000 ALCOHOL/ETHANOL LIQUID COMMODITIES ORIGINATING FROM RCPE STATIONS AMENDMENT 12
RAPID CITY, PIERRE & EASTERN RAILROAD RCPE TARIFF 4000 ALCOHOL/ETHANOL LIQUID COMMODITIES ORIGINATING FROM RCPE STATIONS AMENDMENT 12 ISSUED: February 15, 2018 1 P a g e TABLE OF CONTENTS SECTION ONE:
More informationAPPLICATION OF SUPERCRITICAL FLUIDS FOR POLYPHENOLIC COMPOUNDS EXTRACTION FROM EXHAUSTED OLIVE POMACE
Processes APPLICATION OF SUPERCRITICAL FLUIDS FOR POLYPHENOLIC COMPOUNDS EXTRACTION FROM EXHAUSTED OLIVE POMACE Ashley Sthefanía Caballero 1, Juan Miguel Romero-García 2, Eulogio Castro 2, Carlos Ariel
More informationProduction of phenolic antioxidants from apple residue using Rhizopus oligosporus
Available online at http://www.ijabbr.com International journal of Advanced Biological and Biomedical Research Volume 2, Issue 6, 2014: 1937-1942 Production of phenolic antioxidants from apple residue
More informationPAPRIKA EXTRACT SYNONYMS DEFINITION DESCRIPTION FUNCTIONAL USES CHARACTERISTICS
PAPRIKA EXTRACT Prepared at the 77 th JECFA, published in FAO JECFA Monographs 14 (2013), superseding tentative specifications prepared at the 69 th JECFA (2008). An ADI of 0-1.5 mg/kg bw was allocated
More information