Taking inspiration from Nature: Biomimicry and Bioflavonoids from propolis
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- Samson McCarthy
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2 Taking inspiration from Nature: Biomimicry and Bioflavonoids from propolis Propolis is a mixture of substances secreted by plants in order to protect their buds from several exogenous factors like: weather bacteria and microorganisms oxidizing agents ultraviolet radiations Polyphenols The chemical composition of propolis: 4-6% Resins -3% Beeswax, vegetable waxes, oils 3-7% Essential oils, volatile substances 3-7% Sugars 3-5% Polyphenols (Bioflavonoids and Phenolic Acids) Phenolic acids Aglycon bioflavonoids Glycosides The polyphenols portion is the one which is responsible for the protection from exogenous agents. Our research has focused precisely on that part, investigating on possible similarities between the positive effects registered in the vegetable kingdom and the cosmetic efficacy (Biomimicry concept). Flavocare : what is it? FLAVOCARE, standardized extracts from propolis in which the bio-active polyphenolic combination (phenolic acids, aglycon bioflavonoids, glycosides) is preserved, has been obtained through a high efficiency patented process, combining selective extraction and standardization (M.E.D. - Dynamic Multi Extraction). Among the polyphenols there are Apigenin, Chrysin, Galangin, Pinocembrin, Pinobanksin, Quercetin, which represent more than 5% of the total composition (analysis performed with double titration in HPLC-ESI-MS and UV-FolinCiocalteau). Extract in ethanol 5-% of propolis MED Extract at 5-% of propolis Waxes and resins that reduces Poliphenols bioactivities HPLC-ESI-MS FLAVOCARE is a high-quality product that does not contain any impurity which could be present in propolis. We guarantee that every production batch is free of more than 3 molecules of contaminants selected among the substances that are used in the countries of origin of propolis: Pesticides Aflatoxins Heavy Metals Rich in soluble glycosylated polyphenols M.E.D., the innovative method of production patented by BNatural srl, allows to extract active substances of different solubility, ensuring high reproducibility and standardization for each production batch.
3 3 Cosmetic efficacy: Flavocare has been tested by means of in-vitro tests as well as in-vivo tests In-VITRO Test: Bacterial Inhibition Flavocare Hydroglyceric has been tested on some bacterial strains at different concentrations to determine respectively MIC (Minimum Inhibitory Concentration) and MCB (Minimum Bactericidal Concentration). Bacterial Strains MIC ( %) MCB (%) Propionibacterium Acnes,5 4, Candida Albicans 4 Staphylococcus Aureus,5 Staphyloccocus Hominis,5 Flavocare Hydroglyceric does possess a reproduction inhibitory effect on the bacterial species that were analyzed. This activity suggests using it in some specific cosmetic applications, ensuring some specific bacterial inhibition activity, while respecting the bacterial flora naturally present on the epidermis. In-VITRO Test: Anti-inflammatory Activity The anti-inflammatory activity has been evaluated regulating the pro-inflammatory marker (TNFalpha) in: Non-treated cell cultures (negative control, CTR-) Cell cultures in which an event of acute inflammation is induced (positive control, CTR+) Cell cultures in which an event of acute inflammation is induced and treated with Flavocare at the same time p<, 8 8, -9,6% TNFAlpha pg/ml 6 4 5, 5,8 CTR- CTR+ Flavocare,5mg/cm Flavocare has an effective impact on cell culture induced inflammation of human fibroblasts, reducing the release of TNFalpha pro-inflammatory cytokine (monitored through Elisa s essay) and demonstrating a high anti-inflammatory activity. The results are expressed as % variation compared to CTR- (-9,6%).
4 4 In-VIVO Test: Deodorizing Power The deodorizing power, suggested by the encouraging results of the bacterial inhibition tests on the Staphyloccocus Hominis strain, has been evaluated through in-vivo SNIFF TEST. Test protocol consists on observations at 8h and 4h from the application of one deodorant micro-emulsion containing,5% Flavocare hydroglyceric on a panel of subjects. Test were conducted under controlled conditions by asymmetrical application (treated armpit/ non-treated contra-lateral armpit) The objective evaluation has been realized awarding a value on the base of an evaluation scale from to 5, each of the values corresponding to different odor perceptions: = very intense odour = intense odour = moderate odour = low odour = very low odour = no sweat odour The clinical evaluation has been performed by three experts focusing on: Deodorization efficacy Fragrance persistency Bad odours inhibition Objective Evaluation Subjective Evaluation 5 5 Evaluation scale 4 3,7,3 T,3 T8h,63,3 T4h 3,53 Evaluation scale 4 3,5,5 T, T8h,7, T4h 3, FLAVOCARE NOT TREATED The formula containing Flavocare Hydroglyceric has shown an odour reduction of 6%. Flavocare offer an unique opportunity to formulate safe, natural & efficient Deodorants.
5 5 In-VIVO Test: Reduction of acneic lesion This cosmetic activity, inspired by the bacterial inhibition tests on the Propionibacterium Acnes strain, has been evaluated in-vivo through daily applications (morning/night for 8 days) of an emulsion containing 4% of Flavocare Hydroglyceric. Tests has been conducted on 34 volunteers of both sexes (7 of them constituted the control group who was treated with the placebo), in the age range 8-4 with Acne-Prone Skin. Three different evaluations has been performed: Acneic lesions evaluation, Sebometric evaluation (Sebometer sm85), High resolution picture with,5 light normalization. For the acneic lesion evaluation the IGA-FDA Acne Severity scale was used. Values range between and 4: = skin without lesions 4= severe acne The results after the treatment with Flavocare show a decrease of 6% in acneic lesions Acne Severity scale (IGA),5,5,8 Days T T3 Delta, -6%,8,5, Placebo Flavocare High resolution pictures before and after the treatment: T T3 T T3 Volunteer 6 Volunteer Volunteer Volunteer 4 From the results obtained by sebometric evaluation, Flavocare has demonstrated its effectiveness in the reduction of sebum production. This activity suggests the possibility of using it in other cosmetic fields where the normalization of sebaceous production is crucial. Percentage variation of sebum,% -5,% -,% -5,% -,% -5,% -3,% -35,% -4,% -45,% Sebum reduction mean variation % -6,8% -39,57% Flavocare Placebo
6 6 Why to use FLAVOCARE? UNIQUE CHEMICAL CHARACTERIZATION Integrity of the polyphenols profile of a natural product TITRATED IN POLYPHENOLS Quality and quantity of polyphenols guaranteed for each batch 3 WITHOUT CONTAMINANTS Resins and waxes eliminated and pesticide and polluting substances under control TESTED 4 EFFICACY Antibacterial effective on odour, acne, intimate hygiene. Anti-inflammatory and sebum regulating 5 FLEXIBILITY OF FORMULATION Compatible with all formulations FLAVOCARE HYDROGLYCERIC Natural raw material Multifunctional ingredient: deodorant, reduction of acneic lesions, sebo regulator activities proven by in-vivo test -5 mg/ml total Polyphenols content M.E.D. Innovative Production Process High solubility in acqueous system Antibacterial & Anti-Inflammatory activities tested in-vitro Antioxidant activity by DPPH test
7 7 FLAVOCARE HYDROGLYCERIC Inci Appearance Titration Fields of application Glycerin, Aqua, Propolis Extract Liquid, pale to amber colour, characteristic odour -5 mg/ml total Polyphenols Face care Body care AP/Deo Intimate Hygiene Recommended Dosage Treatment of acneic lesions 4,% Antiodour Products,5% Intimate Hygiene,5% - 4,% Soothing Product,5% - 4,% Technical information Soluble in ethanol, partially soluble in a hydroalcoholic solution Storing Condition In suitable packaging at a temperature between 5 C and 3 C Shelf life Producer Certification 3 years B NATURAL SRL (Corbetta MI) COSMOS Approved FLAVOCARE OIL 3 Inci Appearance Titration Glycine soja oil, Propolis extract Liquid, yellow to brown colour, characteristic odour >3 mg/ml total Polyphenols Recommended Dosage,8-,% FLAVOCARE POWDER Inci Appearance Titration Acacia Senegal Gum, Propolis extract, Sucrose, Silica Powder >mg/ml total Polyphenols Recommended Dosage,5-,4%
8 ciellecomunicazione.it Kialab Srl C/o Insubrias Biopark Via Lepetit, 34 4 Gerenzano (VA) - Italy Tel Fax info@kialab.it 3 a ristampa Although all statements and information in this advertisement are believed to be accurate and reliable, they are presented gratis and for guidance only, and risks and liability for results obtained by use of the products or application of the suggestions described are assumed by the user. The claims and supporting data provided in this publication have not been evaluated for compliance with any jurisdiction s regulatory requirements and the results reported may not be generally true under other conditions or in other matrices. Users must evaluate what claims and information are appropriate and comply with a jurisdiction s regulatory requirements. SELLER MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, BY FACT OR LAW, INCLUDING WARRANTIES OR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.
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