Transmission of HIV-+ via Breast Milk

Transcription

1 ,**1 The Japanese Society for AIDS Research The Journal of AIDS Research : Transmission of HIV-+ via Breast Milk Hidemi TAKAHASHI Department of Microbiology and Immunology, Nippon Medical School : HIV-+ colostrum BrMM : breast milk macrophage DC DC-SIGN -* /* + nevirapine, nevirapine /* - nevirapine AZT -TC. HIV HIV / free virus HIV HIV 0 HIV CD. CCR/ R/ 1 R/ HIV-+ NL AD2 2 : *, + + /,** * IgA colostrum milk Ficoll-Hypaque Flow Cytometry 3 PBMo : lipid particles + flow cytometry CD+. maturation marker CD2- HIV-+ DC-SIGN +*, 0* BrMM : breast milk macrophage GM-CSF - IL-. CD+. CD+. IL

2 H Takahashi : Transmission of HIV-+ via Breast Milk allogenic MLR T IgA GM-CSF IL BrMM GM-CSF, DC-SIGN 3 DC-SIGN HIV +, +,

3 The Journal of AIDS Research Vol. 3 No. +,**1. IL-. BrMM, HIV-+ HIV-+ CD. ++ T CD - CD+. / CD. CD+. BrMM HIV-+ CCR/ CXCR. R/-type NL AD2 CD. CD+. IL-. HIV-+ IL-. 0A CD. CCR/ CXCR. MAGIC-/ +, DC-SIGN IL-. 0B DC-SIGN Free Virus MAGIC-/ HIV-+ DC-SIGN DC-SIGN NL AD2 DC-SIGN BrMM Free Virus wash out DC-SIGN IL-. 1 BrMM DC-SIGN R/-type HIV-+ Free Virus - HIV SIV HIV-+ T +- +-

4 H Takahashi : Transmission of HIV-+ via Breast Milk HIV-+ T ph -.* HIV-+ Free + 2A DC-SIGN HIV-+ - -*.* 2B / CD. J Infect Dis, +3+ : ,**/ 1 DC-SIGN J Infect Dis, +3+ : ,**/ 0 BRMM HIV-+ J Infect Dis, +3+ : ,**/ +. +.

5 The Journal of AIDS Research Vol. 3 No. +,**1 2 HIV J Infect Dis, +3+ : ,**/ Free Virus DC-SIGN HIV-+ T IgA nevirapine - 3 Free Virus IL-. DC-SIGN HIV-+ IL-. DC-SIGN + Nduati R, John G, Mbori-Ngacha D, Richardson B, Overbaugh J, Mwatha A, Ndinya-Achola J, Bwayo J, Onyango FE, Hughes J. Kreiss J : E#ect of breastfeeding and formula feeding on transmission of HIV-+ : a randomized clinical trial. Jama,2- : ,,***., Musoke P, Guay LA, Bagenda D, Mirochnick M, Nakabiito C, Fleming T, Elliott T, Horton S, Dransfield K, Pav JW, Murarka A, Allen M, Fowler MG, Mofenson L, Hom D, Mmiro F, Jackson JB : A phase I/II study of the safety and pharmacokinetics of nevirapine in HIV-+infected pregnant Ugandan women and their neonates (HIVNET **0). Aids +- :.13.20, McIntyre J : Strategies to prevent mother-to-child transmission of HIV. Curr Opin Infect Dis +3 : -- -2,,**0.. Dabis F, Leroy V, Castetbon K, Spira R, Newell ML, Salamon R : Preventing mother-to-child transmission of HIV-+ in Africa in the year,***. Aids +. : +*+1 +*,0,,***. / Shapiro RL, Holland DT, Capparelli E, Lockman S, Thior I, Wester C, Stevens L, Peter T, Essex M, Connor JD, Mirochnick M : Antiretroviral concentrations in breastfeeding infants of women in Botswana receiving antiretroviral treatment. J Infect Dis +3, : 1,* 1,1,,**/. 0 Rousseau CM, Nduati RW, Richardson BA, Steele MS, John-Stewart GC, Mbori-Ngacha DA, Kreiss JK, Over- +/ +/

6 H Takahashi : Transmission of HIV-+ via Breast Milk baugh J : Longitudinal analysis of human immunodeficiency virus type + RNA in breast milk and of its relationship to infant infection and maternal disease. J Infect Dis +21 : ,,**-. 1 Becquart P, Chomont N, Roques P, Ayouba A, Kazatchkine MD, Belec L, Hocini H : Compartmentalization of HIV-+ between breast milk and blood of HIV-infected mothers. Virology -** : +*3 ++1,,**,. 2 Englund G, Theodore TS, Freed EO, Engleman A, Martin MA : Integration is required for productive infection of monocyte-derived macrophages by human immunodeficiency virus type +. J Virol 03 : -,+0 -,+3, +33/. 3 Ichikawa M, Sugita M, Takahashi M, Satomi M, Takeshita T, Araki T, Takahashi H : Breast milk macrophages spontaneously produce granulocyte-macrophage colonystimulating factor and di#erentiate into dendritic cells in the presence of exogenous interleukin-. alone. Immunology +*2 : /,,**-. +* Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y : DC-SIGN, a dendritic cell-specific HIV-+binding protein that enhances trans-infection of T cells. Cell +** : /21 /31,,***. ++ Satomi M, Shimizu M, Shinya E, Watari E, Owaki A, Hidaka C, Ichikawa M, Takeshita T, Takahashi H : Transmission of macrophage-tropic HIV-+ by breast-milk macrophages via DC-SIGN. J Infect Dis +3+ : ,,**/. +, Hachiya A, Aizawa-Matsuoka S, Tanaka M, Takahashi Y, Ida S, Gatanaga H, Hirabayashi Y, Kojima A, Tatsumi M, Oka S : Rapid and simple phenotypic assay for drug susceptibility of human immunodeficiency virus type + using CCR/-expressing HeLa/CD. ( ) cell clone + +* (MAGIC-/). Antimicrob Agents Chemother./ :.3/ /*+,,**+. +- Veazey RS, DeMaria M, Chalifoux LV, Shvetz DE, Pauley DR, Knight HL, Rosenzweig M, Johnson RP, Desrosiers RC, Lackner AA : Gastrointestinal tract as a major site of CD. T cell depletion and viral replication in SIV infection. Science,2* :.,1.-+, Kuribayashi H, Wakabayashi A, Shimizu M, Kaneko H, Norose Y, Nakagawa Y, Wang J, Kumagai Y, Margulies DH, Takahashi H : Resistance to viral infection by intraepithelial lymphocytes in HIV-+ P+2-I+*-specific T- cell receptor transgenic mice. Biochem Biophys Res Commun -+0 : -/0-0-,,**