- Determining the Causes - Dr. Diana Cox-Foster Pennsylvania State University
|
|
- Diane Freeman
- 5 years ago
- Views:
Transcription
1 Colony Collapse Disorder - Determining the Causes - Dr. Diana Cox-Foster Pennsylvania State University
2 Honey Bees in US Agriculture and the Colony Collapse Disorder Essential for pollination of over 100 fruit and vegetable crops in the United States Valued at more than $14.6 billion in the U.S. Varroa mites have resulted in a major decrease in managed colonies in last 18 yrs Recent increased death of colonies -- termed Colony Collapse Disorder (CCD)) CCD affects an estimated 1/4 of all US beekeepers, causing 35 to 100% loss of colonies in operations
3 Symptoms of CCD Rapid decline in number of adult bees Queens present with a few young adult bees, lots of larvae/pupae, and abundant food resources No dead adult bees in or outside the colony No robbing by other bees or invasion by pest insects, suggesting presence of a toxin Infection by an extremely large number of different disease organisms in each adult
4 CCD HEALTHY
5 CCD Working Group Goals Identify potential causal factors common to CCD colonies and not associated with strong, healthy bee colonies Determine how these factors can underlie CCD, by experimentally reproducing CCD symptoms Devise preventative measures to disrupt CCD and ensure strong colonies for pollination
6 Preliminary Work on CCD Description of CCD symptoms Examine known pathogens and parasites Document hive conditions Surveys of Beekeepers Collection of samples from both CCD and non-ccd beekeeping operations Agreement to extensively share samples and data
7 What are the known pathogens of honey bees?
8 Danger, danger, lurking everywhere---
9 A bee s encounter with pathogens and parasites Varroa mites Fungal spores Viruses Nosema Bacteria FOOD Tracheal mites
10 Insect Immunity to Pathogens and Wounding Wounding and melanotic encapsulation Midgut Enzymes and antimicrobial peptides Fat Body Ovary Saliva (glucose oxidase) Hemolymph and blood cells
11 Varroa mite parasitizes the developing pupa and reproduces in the brood cell.
12 How do mites impact honeybee health? Increased incidence of diseases following mite introduction Suppress the immune system of bees, even after removal Infected by some bee viruses Vector viruses
13 Increased incidence of chalkbrood fungal disease in PA following varroa introduction Dennis VanEnglesdorp, State Bee Inspector, October 2005
14 Mites affect specific aspects of honeybee immunity Cellular immunity Lack of key enzyme at adult emergence Decreased enzymes needed for killing reaction Increased cell reaction Antimicrobial reaction Decreased production of antimicrobial peptides
15 Viruses and Varroa--- Role in honey bee disease and colony health???? 18 honey bee viruses Most are picornalike viruses Sacbrood bee virus (SBV) Deformed wing virus (DWV) Kashmir bee virus (KBV) Black queen cell virus (BQCV) Acute bee paralysis virus (ABPV)
16 Bee viruses can be latent or persistent and not cause visible symptoms, as seen with deformed wing virus. No mites, normal wings Mites, normal wings Mites, deformed wings Degree of Wing Deformity Y = * X R 2 = Number of Mites with Bee during Development
17 Deformed-winged bees: A combination of mites and microbial interaction to produce high viral levels? DWV RNA relative to Actin mrna E-3 No injection Saline E. coli Saline E. coli Saline E. coli Without mites, Normal Wings With mites, Normal Wings With mites, Deformed Wings
18 Hypothesized interactions- Varroa mites Immune Normal defenses colonies to without bacteria mites are are decreased able to maintain due to immunosuppression immune defenses by mite to bacteria. feeding Bacteria Viruses replicate Viruses following like DWV, mite SBV, and parasitization. KBV may established Through a an persistent unknown infection, mechanism, that may microbe in the case exposure of DWV triggers be positively a large selected increase by in DWV the in bee. particular. Viruses- DWV, SBV and KBV
19 Percent DWV Hive Hive Hive Hive16 13 w 13 d 13 e 14 w 14 d 14 e 15 w 15 d 15 e 16 w 16 d 16 e Virus prevalence increases during the summer, when mites are not controlled Hive (Day of year) 17 w 17 d 17 e Workers Drones Eggs
20 EVIDENCE OF TRANSMISSION ROUTES Queen Bee Eggs Honey, royal jelly, or bee bread Brood food Larvae Pupae TO OTHER COLONIES VIA CONTAMINATED EQUIPMENT (OR HONEY)? Workers Sperm, Seminal fluid Drones Varroa Mites TO OTHER COLONIES?
21 How does the combination of mites and chemical stress impact viruses in honey bees?
22 Date Treatment date Coumaphos Mite Drop / Day Threshold - 50 mites/day Formic Pad WV Fume Board Untreated
23 % BQCV Infection Airport Apiary 0 Week 2 Week 10 Week Chemical mite controls differentially affect viral infections-- Suppression of DWV versus Activation of BQCV 0 Untreated WV Fume Board Formic Pad Coumaphos
24 What type of pathogens do we know CCD bees have?
25 Photo by Dennis vanengelsdorp
26 Photo by Dennis vanengelsdorp
27 Working Hypotheses for potential causes of CCD Are new or reemerging pathogens responsible for CCD? Are environmental chemicals impacting bees and triggering CCD? Is a combination of stresses weakening bee colonies and allowing stressrelated pathogens to cause final collapse?
28 Breeding New Genetic Strains of Honey Bees Need for increased genetic resistance to diseases and the impacts of parasites
29 Do we need to be worried about the causal agents of CCD impacting other insect pollinators?
30 SBV DWV HIVE 5 HIVE 46 A B C A B C A single cell of bee bread can have virus present Other Bee Bread Cells w/ DWV HIVE 5 HIVE 46 HIVE 43 in single layers of different colored pollen Hive(Chi Square 1.493, p = ); Color of Pollen (Chi Square=1.452,p = )
31 DWV is NOT associated with a particular color of pollen 50 Percent DWV Black/Brown Brown Light brown Yellow Pollen Color Color of Pollen (Chi Square=1.452,p = )
32 When is the pollen becoming contaminated by the bee viruses?
33 Honey bee viruses --- pollen vs. pollen foragers Pollen DWV SBV DWV- H/Th1 Pollen baskets collected with pollen foragers; foragers either immediately frozen or held 24 hrs with food/h 2 O RNA extracted from pollen and foragers separated into Head/Th1 and Th2,3/Abdomen; RT-PCR performed for DWV, SBV, actin Pollen Foragers DWV- Th/Ab SBV- H/Th1 SBV- Th/Ab Actin- H/Th1 Actin- Th/Ab Retained 24hrs 0 hrs Pollen- DWV 5/12 positive 3/5 from DWV- foragers SBV 11/12 positive Foragers W/positive pollen 0/12 with DWV infection associated with salivary glands (SG) in H/Th1 sample 1/12 with SBV assoc. with SG W/O positive pollen Lack of DWV in pollen of heavily infected H/Th1 of forager
34 Transmission of viruses among pollinators??? Apis mellifera Flowering Plants?? Queen Bee Honey (royal jelly or bee Brood bread) food Eggs Larvae Pupae Bombus or other pollinators Worke rs (Seminal fluid?) Drone s Varroa Mites
35 Deformed wing virus in bumblebees -- can it be acquired from honey bees? Genersch E., Yue C., Fries I., and de Miranda J.R. Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities. Journal of Invertebrate Pathology 91 (2006) 61 63
36 Penn State University Colony Collapse Disorder Working Group Pennsylvania Department of Agriculture USDA-ARS, Bee Research Laboratory, Beltsville, MD Columbia University and Northeast Biodefense Center Florida Department of Agriculture and Consumer Services North Carolina State University University of Illinois, Champaign-Urbana University of Delaware Additional Research Bee Alert Technologies, Inc., Montana and affiliates
37 Summary Extensive collaboration among researchers of diverse expertises and affiliations Federal, State, and Land-Grant University research targeting real-world problems Cooperative Extension providing the bridge between the research community and the beekeepers and those dependent on pollination Challenges in unraveling the causes of CCD Critical need for prevention to ensure the health of bees (and all insect pollinators) and the pollination industry
38 Questions?
Colony Collapse Disorder (CCD) affecting honey bee (Apis mellifera) colonies
Colony Collapse Disorder (CCD) affecting honey bee (Apis mellifera) colonies Dr. Jeff Pettis USDA-ARS Bee Research Lab. Beltsville, Maryland, USA Bee Health and Symptoms of CCD Explore potential causes
More informationPests and Pathogens. Varroa and Viruses. Orlando Yañez Peter Neumann. Institute of Bee Health University of Bern
Pests and Pathogens Varroa and Viruses Orlando Yañez Peter Neumann Institute of Bee Health University of Bern What is causing bees to decline? GM crops Pesticides Poor beekeeping management Pollution Electromagnetic
More informationDeformed Wing Virus (DWV) infection. Impact on honey bee queen health
Federal Departement of Economic Affairs DEA Agroscope Liebefeld-Posieux Research Station ALP Deformed Wing Virus (DWV) infection Impact on honey bee queen health Laurent Gauthier Sept. 18 / 2009 Introduction
More informationThe prime directive. The Four Golden Rules of Beekeeping. Maximize the size the adult population
Presented by Lance Wilson Certified Master Beekeeper University of Georgia/Young Harris The prime directive Maximize the size the adult population WHY? 1. Protects against robbing 2. Prevents SHB and wax
More informationDisease Complex of the Honey Bee
Rick Fell, Department of Entomology, Virginia Tech Bacterial diseases Fungal diseases Viral diseases Protozoan diseases Disease Complex of the Honey Bee Viral diseases: 24 identified; 6 common Bacterial
More informationNational Honey Bee Disease Survey Report
2014 2015 National Honey Bee Disease Survey Report Shayne Madella 1, Karen Rennich 1, Jay Evans 2, Samuel Abban 2, Heather Eversole 1, Ashrafun Nessa 1, Rachel Fahey 1, Dawn Lopez 2, Todd Waters 1, Anthony
More informationMCNAIR PROGRAM HONEY BEE BEHAVIORS & VIRUSES
HONEY BEE BEHAVIORS & VIRUSES Author: Amber E. Haritos Co-authors: Drs. Amy L. Toth and Adam G. Dolezal Iowa State Department of Ecology, Evolution and Organismal Biology BACKGROUND Over 80% of plants
More informationHoney Bee Brood Disease, Detection and Management
Honey Bee Brood Disease, Detection and Management Samuel K. Abban Bee Research Laboratory Beltsville, Maryland Introduction Focus my discussion on the major brood diseases Diagnosis and treatment options
More informationUsing causal analysis for evaluating environmental and health issues
Using causal analysis for evaluating environmental and health issues Charles Menzie Ph.D. 2015 camenzie@exponent.com Risk Assessment Seminar Series at TERA Center Z150329-5264 What has caused biological
More informationHoney bee diseases, pests, and disorders
Honey bee diseases, pests, and disorders David R. Tarpy CES Agent Training Assistant Professor and Extension Apiculturist Department of Entomology, Campus Box 7613 North Carolina State University Raleigh,
More informationPest and Disease Treatments Varroa Mites: (Varroa Destructor) Treatments: European Foulbrood: Treatment:
Pest and Disease Treatments Varroa Mites: (Varroa Destructor) Infested capped drone brood Disfigured adult bees, deformed legs or wings Bees discarding larvae and pupae Pale or dark reddish brown spots
More informationEXPERIMENTAL INFECTION OF WINTER WORKER BEES (APIS MELLIFERA CARNICA) WITH CHRONIC BEE PARALYSIS VIRUS (CBPV), STRAIN M92/2010
EXPERIMENTAL INFECTION OF WINTER WORKER BEES (APIS MELLIFERA CARNICA) WITH CHRONIC BEE PARALYSIS VIRUS (CBPV), STRAIN M92/21 Aleš Gregorc 1, Urška Jamnikar Ciglenečki 2, Mitja Nakrst 1, Ivan Toplak 2 1
More informationHoneybee Diseases and Parasites Bob Arnold March 5, 2008
Honeybee Diseases and Parasites Bob Arnold March 5, 2008 1 Major Honeybee Diseases Bacterial Diseases American Foulbrood-AFB European Foulbrood-EFB Fungal Diseases Chalkbrood Viral Diseases Kashmir Bee
More informationAmerican Foulbrood (AFB) in South Africa
American Foulbrood (AFB) in South Africa Mike Allsopp & Teresa Goszczynska ARC-Plant Protection Research Institute Stellenbosch & Pretoria South Africa AFB in Africa? Until recently there was only one
More informationThe Effect of Oxalic Acid Treatments on Queen Survival and Drone Semen Viability
The Effect of Oxalic Acid Treatments on Queen Survival and Drone Semen Viability Project No.: Project Leader: Project Coordinators: 08-POLL5-Ellis Marion D. Ellis University of Nebraska 312 N. 14thStreet
More informationNew Method for American Foulbrood Disease Control. Hossein Yeganehrad Caspian Apiaries
New Method for American Foulbrood Disease Hossein Yeganehrad Caspian Apiaries Introduction American Foulbrood [AFB] disease is and endemic and epidemic bacterial disease caused by the Paenibacillus larvae.
More informationDETECTION OF HONEY BEE VIRUSES IN JORDAN
DETECTION OF HONEY BEE VIRUSES IN JORDAN Nizar Haddad Bee Research Unit & Biotechnology Unit. National Center for Agricultural Research and Extension. Baqa' 19381 Jordan, drnizarh@yahoo.com. www.jordanbru.info
More informationMiaoqing Shen, Liwang Cui, Nancy Ostiguy and Diana Cox-Foster INTRODUCTION
Journal of General Virology (2005), 86, 2281 2289 DOI 10.1099/vir.0.80824-0 Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee
More informationNosema Hidden Killer. Another explanation for bee losses By Mike Sandridge
Nosema Hidden Killer Another explanation for bee losses By Mike Sandridge CASE STUDY I m going to be your case study A example case Package Bees from GA, on used and new comb Nucs from hives with VA queens
More informationFirst detections of honey bee pathogens in nest of the Asian hornet (Vespa velutina) collected in France
First detections of honey bee pathogens in nest of the Asian hornet (Vespa velutina) collected in France Marie-Pierre Chauzat ANSES. Scientific Affairs Department for Laboratories. Unit of coordination
More informationSpring Management of Honeybees Part 1 Population Dynamics, Varroa
About Capital Bee Supply Spring Management of Honeybees Part 1 Population Dynamics, Varroa Richard Schneider Capital Bee Supply, LLC Columbus, WI 608-444-1493 www.capitalbeesupply.com Manufacturers and
More informationHow to collect raw honeybee pollen from the hive
Published on TECA (http://teca.fao.org) How to collect raw honeybee pollen from the hive SUMMARY: Pollen is collected by the honeybees from the anthers of flowers while they visit them. Pollen is stored
More informationControl of Varroa mites in honey bees through the systemic application of essential oils.
Control of Varroa mites in honey bees through the systemic application of essential oils. Project Number: 05-DS-02 Project Leaders: Gordon Wardell, CRADA Partner USDA-ARS Carl Hayden Bee Research Center,
More informationTolerance to virus infections could explain increased winter colony survival observed in. Varroa destructor-resistant honey bees. Sofia Bouro Wallgren
Institutionen för kvinnors och barns hälsa Biomedicinska analytikerprogrammet Examensarbete 15 hp Tolerance to virus infections could explain increased winter colony survival observed in Varroa destructor-resistant
More informationTHE ROLE OF DEFORMED WING VIRUS IN THE MORTALITY OF VARROA INFESTED HONEYBEE COLONIES
THE ROLE OF DEFORMED WING VIRUS IN THE MORTALITY OF VARROA INFESTED HONEYBEE COLONIES Stephen Martin, email: s.j.martin@sheffleld.ac.uk Brenda Ball l, Norman Carreck'' ILaboratory of Apiculture and Social
More informationThe Pennsylvania State University. The Graduate School. Department of Entomology ECOLOGY AND EPIDEMIOLOGY OF RNA VIRUSES IN HYMENOPTERAN POLLINATORS
The Pennsylvania State University The Graduate School Department of Entomology ECOLOGY AND EPIDEMIOLOGY OF RNA VIRUSES IN HYMENOPTERAN POLLINATORS A Dissertation in Entomology by Rajwinder Singh 2011 Rajwinder
More informationEPILOBEE The EU surveillance study in Hungary
EPILOBEE The EU surveillance study in Hungary 2012-2013 Petra Paulus dr. (National Food Chain Safety Office Veterinary Diagnostic Directorate Laboratory of Parasitology, Fish and Bee Diseases) Paris, 9th
More informationVarroa Mite Detection Methods
Varroa Mite Detection Methods Apiculture Bulletin # 222 Updated: 10/15 Effective mite control is dependent on frequent and reliable mite detection. Varroa mites spread rapidly between hives and apiaries
More informationVIRUS TASKFORCE. Proceedings
In partnership with VIRUS TASKFORCE Proceedings Avignon, France, 6-7/04/2017 VIRUS TASKFORCE Topic The research on honey bee viruses is a quickly expanding frontier and there is an increasing need to address
More informationAssessing Pesticide Risks to Bees
U.S. Environmental Protection Agency 1 Assessing Pesticide Risks to Bees Environmental Fate and Effects Division Office of Pesticide Programs January 2015 U.S. Environmental Protection Agency 2 Briefing
More informationIntroduction to Queen Rearing
Introduction to Queen Rearing Heather Blackwell Graduate Research Assistant MSU Apiculture Mississippi Agricultural & Forestry Experiment Station Members of the Colony Drones are reproductive & haploid
More informationTel: ; Fax: URL: Cohen, E., Ph.D. (Head of Department) Shafir, S., Ph.D.
ENTOMOLOGY Tel: 972-8-948-9223; Fax: 972-8-946-6768 URL: http://departments.agri.huji.ac.il/entomology/ STAFF Professors Emeriti: Gerson, U., Ph.D. Lensky, Y., Ph.D. Applebaum, S.W., Ph.D. 1 Professors:
More informationThe Effect of Oxalic Acid Treatments on Drone Semen Viability and Queen Survival, Egg Laying Rate, Egg Viability and Brood Production
The Effect of Oxalic Acid Treatments on Drone Semen Viability and Queen Survival, Egg Laying Rate, Egg Viability and Brood Production Project No.: Project Leader: 08-POLL5-Ellis Marion Ellis University
More informationPrevalence and Seasonal Variations of Six Bee Viruses in Apis mellifera L. and Varroa destructor Mite Populations in France
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Dec. 2004, p. 7185 7191 Vol. 70, No. 12 0099-2240/04/$08.00 0 DOI: 10.1128/AEM.70.12.7185 7191.2004 Copyright 2004, American Society for Microbiology. All Rights
More informationFoulbrood Bee Diseases
Foulbrood Bee Diseases Healthy Brood- Plump, pearly white Healthy Brood Brood diseases prevent the development of normal healthy replacement adult bees. Both European and American Foulbrood diseases are
More informationHONEY BEE NUTRITION Les Jeske Maple Creek Honey Farm Tyler, Texas
HONEY BEE NUTRITION Les Jeske Tyler, Texas Primary Needs of Bees Nutrition Pest Control Nutritional Requirements Water Protein (Amino Acids) Fats/Lipids (Fatty Acids & Sterols) Carbohydrates (Sugar & Starches)
More informationMonitoring it does make a difference by Dewey M. Caron
Monitoring it does make a difference by Dewey M. Caron Varroa mites continue to change the beekeeping landscape. In the good ol days it was far easier to start a bee colony and keep them alive than today.
More informationCANADIAN NATIONAL HONEY BEE HEALTH SURVEY
CANADIAN NATIONAL HONEY BEE HEALTH SURVEY Photo Credit: Christy Curran 2016 REPORT British Columbia, Alberta, Manitoba, Ontario, Quebec, New Brunswick, Nova Scotia, Prince Edward Island, Newfoundland &
More informationBEENET: ITALIAN BEEKEEPING MONITORING NETWORK PRELIMINARY DATA
BEENET: ITALIAN BEEKEEPING MONITORING NETWORK PRELIMINARY DATA Porrini C 1, Lodesani M 2, Libertà A 3, Bortolotti L 2, Gallina A 4, Colombo R 2, Sgolastra F 1, Medrzycki P 2, Bozza MA 4, Mutinelli F 4
More informationA pan-european epidemiological study on honeybee colony losses
A pan-european epidemiological study on honeybee colony losses 2012-2013 Marie-Pierre CHAUZAT, Marion LAURENT, Marie-Pierre RIVIERE, SAUGEON, Pascal HENDRIKX and Magali RIBIERE-CHABERT Cécile European
More informationAgricultural Institute of Slovenia, Ljubljana, Slovenia 2. University of Maribor, Faculty of Agriculture and Life Sciences, Slovenia 3
ACTA VET. BRNO 2012, 81: 015 019; doi:10.2754/avb201281010019 Viral infections in queen bees (Apis mellifera carnica) from rearing apiaries Aleš Gregorc 1,2 and Tamás Bakonyi 3 1 Agricultural Institute
More informationPOLYADENYLATION OF RIBOSOMAL RNA IN RESPONSE TO PICORNAVIRUS INFECTION IN HONEY BEES (APIS MELLIFERA) JOHNNY YU THESIS
POLYADENYLATION OF RIBOSOMAL RNA IN RESPONSE TO PICORNAVIRUS INFECTION IN HONEY BEES (APIS MELLIFERA) BY JOHNNY YU THESIS Submitted in partial fulfillment of the requirements for the degree of Master of
More informationMethods APRIL 2018 HOW DO BEES CHOOSE WHAT TO EAT? P:F* Pollen B. Experiment/ Expectation. Pollen A P:F* 3.5. Natural desert. 1) Pollen A = Pollen B
APRIL 2018 How do bees choose what to eat? Authors: Vanessa Corby-Harris, Lucy Snyder, Charlotte Meador and Trace Ayotte Associate Editors: Elitsa Panayotova, Gogi Kalka Abstract Hard working bees play
More informationDoes Nosema ceranae Infect Bees and Contaminate Honey in Australia?
Does Nosema ceranae Infect Bees and Contaminate Honey in Australia? by Michael Hornitzky October 2008 RIRDC Publication No 08/133 RIRDC Project No PRJ-000885 2008 Rural Industries Research and Development
More informationThe EAS Master Beekeeper Program. Part 2. Self-test questions. The oral and written exams
The EAS Master Beekeeper Program Part 2 Self-test questions The oral and written exams THE ORAL EXAM A sample question might go something like this: I took the beekeeping class this year. It is August
More informationDeformed Wing Virus Implicated in Overwintering Honeybee Colony Losses
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Nov. 2009, p. 7212 7220 Vol. 75, No. 22 0099-2240/09/$12.00 doi:10.1128/aem.02227-09 Copyright 2009, American Society for Microbiology. All Rights Reserved. Deformed
More informationAntioxidants in Honey Bee Colonies
Antioxidants in Honey Bee Colonies Project No.: Project Leader: 09-POLL3-Sammataro Dr. Diana Sammataro USDA-ARS Carl Hayden Honey Bee Research Center 2000 East Allen Road Tucson, AZ 85719 (520) 670-6380
More informationHONEY BEE BIOLOGY Apprentice Level Training Texas Master Beekeeper Program
HONEY BEE BIOLOGY Apprentice Level Training Texas Master Beekeeper Program Anatomy Overview Three tagmata 1. Head Sensory 2. Thorax Locomotion 3. Abdomen Digestion and reproduction Anatomy Overview O =
More informationHoney Bee Biology. Elina L. Niño Extension Apiculturist
Honey Bee Biology Elina L. Niño Extension Apiculturist listverse.com E.L. Niño Honey Bee Lab Bernardo Niño Staff Res. Assoc. Charley Nye Laidlaw Facility Mngr. Patricia Bohls PhD Student Cameron Jasper
More informationGrow Your Own Queen Bees Recap. Presented By: Kevin Lewis
Grow Your Own Queen Bees Recap Presented By: Kevin Lewis Queen Rearing Resources: Michael Bush beekeeping website: http://bushfarms.com/bees.htm Queens -A few good queens & Queen Rearing Michael Palmer
More informationGuidelines for a pilot surveillance project on honeybee colony losses
European Union Reference Laboratory for honeybee health Guidelines for a pilot surveillance project on honeybee colony losses Introduction... 2 1.1 Definitions...3 1.2 EU Legal framework...6 1.2.1 EU RL
More informationDifferential feeding of larvae affects caste differentiation in Apis mellifera
Differential feeding of larvae affects caste differentiation in Apis mellifera Willem J. Boot, Johan N.M. Calis & Mike Allsopp 1 Laboratory of Entomology, Wageningen Agricultural University, PO Box 8031,
More informationEarly-life experience affects honey bee aggression and resilience to immune challenge
SUPPLEMENTARY INFORMATION Early-life experience affects honey bee aggression and resilience to immune challenge Clare C. Rittschof, Chelsey B. Coombs, Maryann Frazier, Christina M. Grozinger, Gene E. Robinson
More informationLocalization of Deformed Wing Virus (DWV) in the Brains of Apis mellifera (European Honey Bees)
Bucknell University Bucknell Digital Commons Honors Theses Student Theses 2009 Localization of Deformed Wing Virus (DWV) in the Brains of Apis mellifera (European Honey Bees) Karan Samir Shah Bucknell
More informationVirus Detection for Honey Bees: the IVDS Rosetta Stone Project
Virus Detection for Honey Bees: the IVDS Rosetta Stone Project Project No.: Project Leader: 08-POLL9-Wick David Wick President/CEO BVS, Inc. 1620 Rodgers St., Suite 2 Missoula, MT 59870 (406) 369-4214
More informationAlternative Honey Bee Nutrition Beyond Sugar Syrup. Petrusia Kotlar DC, Nancy Ostiguy PhD. Abstract
Alternative Honey Bee Nutrition Beyond Sugar Syrup Petrusia Kotlar DC, Nancy Ostiguy PhD Abstract Feeding honeybees syrup of cane-sugar or HFCS during a nectar dearth fails to provide honeybees with the
More informationPrevalence of multiple viral diseases associated with honey bees colony collapse and control of disorders
International Journal of Zoology Studies ISSN: 2455-7269 www.zoologyjournals.com Volume 1; Issue 2; February 2016; Page No. 29-34 Prevalence of multiple viral diseases associated with honey bees colony
More informationFinal Research Report to The National Honey Board. Ramesh Sagili. Department of Horticulture, Oregon State University, Corvallis, OR
Final Research Report to The National Honey Board Ramesh Sagili Department of Horticulture, Oregon State University, Corvallis, OR 97331-7304 Project title: Comprehensive evaluation of role of nutrition
More informationBee-Pro and Feedbee : A comparison of Capped Brood Areas. Jonathan Hofer. Keystone Honeyhouse
Bee-Pro and Feedbee : A comparison of Capped Brood Areas Jonathan Hofer Keystone Honeyhouse Comparing Feedbee and Bee-Pro 2 Abstract The 2009 Pollen Supplement Study investigated the capped brood area
More informationPrevalence and distribution patterns of seven different honeybee viruses in diseased colonies: a case study from Croatia
Apidologie * INRA, DIB and Springer-Verlag France, 2014 DOI: 10.1007/s13592-014-0287-0 Original article Prevalence and distribution patterns of seven different honeybee viruses in diseased colonies: a
More informationOver recent years, a problem has developed with honey bees which
COLONY COLLAPSE DISORDER AND GM CROPS AND GM C The Bt Cry toxins in genetically modified crops are thought to be responsible, at least in part, for impairing the immune response, memory and navigation
More informationThe prophylaxis of Nosema disease in bees by laboratory methods - microscopic testing of the beehive products
Romanian Biotechnological Letters Vol. x, No. x, 018 Copyright 018 University of Bucharest Printed in Romania. All rights reserved ORIGINAL PAPER The prophylaxis of Nosema disease in bees by laboratory
More informationUlcer Treatment with n Chromosome Royal Jelly. Hossein Yeganehrad Caspian Apiaries
Ulcer Treatment with n Chromosome Royal Jelly Hossein Yeganehrad Caspian Apiaries Introduction Stomach ulcers are mostly caused by the bacteria. Helicobacter pylori. n chromosome is the name given to royal
More informationLab Tuesday: Virus Diseases
Lab Tuesday: Virus Diseases Quiz for Bacterial Pathogens lab (pp 67-73) and Biocontrol of Crown Gall (p. 113-117), Observation of Viral Movement in Plants (p. 119), and Intro section for Viruses (pp. 75-77).
More informationAntioxidants in Wax Cappings of Honey Bee Brood
Antioxidants in Wax Cappings of Honey Bee Brood Project No.: Project Leader: 08-POLL3-Sammataro Diana Sammataro, Ph.D. Research Entomologist Carl Hayden Honey Bee Research Center 2000 East Allen Road Tucson,
More informationTHE IMPACT OF THE NUTRITIVE VALUE OF POLLEN ON THE DEVELOPMENT, REPRODUCTION AND PRODUCTIVITY OF HONEY BEE (APIS MELLIFERA L.)
685 Bulgarian Journal of Agricultural Science, 20 (No 3) 2014, 685-689 Agricultural Academy THE IMPACT OF THE NUTRITIVE VALUE OF POLLEN ON THE DEVELOPMENT, REPRODUCTION AND PRODUCTIVITY OF HONEY BEE (APIS
More informationPreliminary observations on possible pathogen spill-over from Apis mellifera to Apis cerana
Apidologie (2015) 46:265 275 * The Author(s), 2014. This article is published with open access at Springerlink.com DOI: 10.1007/s13592-014-0320-3 Original article Preliminary observations on possible pathogen
More informationScreening Swedish bees for pathogens shines new light on the. parasite Gregarine
Institutionen för kvinnors och barns hälsa Biomedicinska analytikerprogrammet Examensarbete 15 hp Screening Swedish bees for pathogens shines new light on the parasite Gregarine Nellie Svedin Abstract
More informationA severe sacbrood virus outbreak in a honeybee (Apis mellifera L.) colony: a case report
Case Report Veterinarni Medicina, 60, 2015 (6): 330 335 A severe sacbrood virus outbreak in a honeybee (Apis mellifera L.) colony: a case report C. Roy 1, N. Vidal-Naquet 2, B. Provost 3 1 Veterinary Clinic,
More informationSubcellular Localization of the Non-Structural Proteins 3C and 3CD of the Honeybee Virus Deformed Wing Virus
Bucknell University Bucknell Digital Commons Honors Theses Student Theses 2010 Subcellular Localization of the Non-Structural Proteins 3C and 3CD of the Honeybee Virus Deformed Wing Virus Cierra Nichole
More informationDengue Virus-Danger from Deadly Little Dragon
Molecular Medicine Dengue Virus-Danger from Deadly Little Dragon Dr.G.MATHAN Assistant Professor Department of Biomedical Science Bharathidasan University Tiruchirappalli, Tamil Nadu Vector (A carrier)
More informationPatterns of viral infection in honey bee queens
Journal of General Virology (2013), 94, 668 676 DOI.99/vir.0.047019-0 Patterns of viral infection in honey bee queens Roy Mathew Francis, Steen Lykke Nielsen and Per Kryger Correspondence Per Kryger per.kryger@agrsci.dk
More informationIridovirus and Microsporidian Linked to Honey Bee Colony Decline
Iridovirus and Microsporidian Linked to Honey Bee Colony Decline Jerry J. Bromenshenk 1,7 *, Colin B. Henderson 2,7, Charles H. Wick 3, Michael F. Stanford 3, Alan W. Zulich 3, Rabih E. Jabbour 4, Samir
More informationTEMPERATURE AND CLIMATE IN CHALKBROOD DISEASE José M Flores Departament of Zoology. University of Córdoba. Spain.
TEMPERATURE AND CLIMATE IN CHALKBROOD DISEASE José M Flores Departament of Zoology. University of Córdoba. Spain. http://www.uco.es/apicultura ba1flsej@uco.es CHALKBROOD A. apis SPORES MICELIUM Chalkbrood
More informationThe Impact of Different Protein Content of Pollen on Honey Bee (Apis mellifera L.) Development
American Journal of Entomology 2018; 2(3): 23-27 http://www.sciencepublishinggroup.com/j/aje doi: 10.11648/j.aje.20180203.11 The Impact of Different Protein Content of Pollen on Honey Bee (Apis mellifera
More informationPreliminary report on the use of Viusid vet liquid in the stimulative feeding of bee colonies
UNIVERSIDAD DE SANCTI SPÍRITUS José Martí Pérez (UNISS) Facultad de Ciencias Agropecuarias Preliminary report on the use of Viusid vet liquid in the stimulative feeding of bee colonies Vicente Méndez García*,
More informationEmerging viral disease risk to pollinating insects: ecological, evolutionary and anthropogenic factors
Journal of Applied Ecology 2015, 52, 331 340 doi: 10.1111/1365-2664.12385 REVIEW Emerging viral disease risk to pollinating insects: ecological, evolutionary and anthropogenic factors Robyn Manley, Mike
More informationKevin Hale
Kevin Hale www.haleshoney.com Bee nutrition and feeding is one of the most important things to learn and understand about honey bees Health of the bees is everything Honey bee nutrition plays a critical
More informationChapter 4: Honey Bee Anatomy
Chapter 4: Honey Bee Anatomy University of Florida Department of Entomology & Nematology Dr. Jamie Ellis Created by: Jason Graham, Catherine Nalen, Jamie Ellis External Morphology Honey bees, like all
More informationCurriculum vitae S. Hollis Woodard. Assistant Professor of Entomology, University of California,
Curriculum vitae Assistant Professor of Entomology University of California, Riverside Mailing address: Department of Entomology University of California, Riverside 900 University Avenue Riverside, CA
More informationRecent spread of Varroa destructor virus-1, a honey bee pathogen, in the United States
www.nature.com/scientificreports Received: 22 September 2017 Accepted: 30 November 2017 Published: xx xx xxxx OPEN Recent spread of Varroa destructor virus-1, a honey bee pathogen, in the United States
More informationAssessment a New Pollen Supplement Diet for Honey Bee Colonies and Their Effects on some Biological Activities
International Journal of Agricultural Technology 2016 Vol. 12(1):55-62 Available online http://www.ijat-aatsea.com ISSN 2630-0192 (Online) Assessment a New Pollen Supplement Diet for Honey Bee Colonies
More informationReceived 13 April 2006/Accepted 30 August 2006
JOURNAL OF VIROLOGY, Dec. 2006, p. 11528 11538 Vol. 80, No. 23 0022-538X/06/$08.00 0 doi:10.1128/jvi.00754-06 Copyright 2006, American Society for Microbiology. All Rights Reserved. Prevalence and Phylogeny
More information1 Cell Of Pollen 1 Cell Of Honey 1 Cell Of Water To Make A Bee
Honey Bee Nutri-on Kim Flo2um Editor, Bee Culture Magazine HONEY BEE NUTRITION ENOUGH GOOD FOOD ALL OF THE TIME FOR EVERY BEE IN THE BUNCH 1 Cell Of Pollen 1 Cell Of Honey 1 Cell Of Water To Make A Bee
More informationLab Tuesday: Virus Diseases
Lab Tuesday: Virus Diseases Quiz for Bacterial Pathogens lab (pp 69-75) and Biocontrol of Crown Gall (p. 115-119), Observation of Viral Movement in Plants (p. 121), and Intro section for Viruses (pp. 77-79).
More informationViral and intestinal diseases detected in Apis mellifera in Central and Southern Chile
Viral and intestinal diseases detected in Apis mellifera in Central and Southern Chile Marisol Vargas 1*, Nolberto Arismendi 1, Gustavo Riveros 1, Nelson Zapata 1, Alex Bruna 1, Mayra Vidal 1, Marta Rodríguez
More informationReasonable Expectations
Reasonable Expectations Tom Chester March 19, 2016 Tom s Beekeeping 1 st Session Beekeeping Now and Over the Long Haul In reality there are. Bee Havers Bee Keepers The first ten years I had bees. I didn
More informationVlaams Diergeneeskundig Tijdschrift, 2008, 78
101 Case Report Vlaams Diergeneeskundig Tijdschrift, 2008, 78 First molecular confirmation of deformed wing virus infections of honeybees from a Belgian apiary reveals the presence of black queen cell
More informationDynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies with Colony Collapse Disorder
Viruses 2014, 6, 2012-2027; doi:10.3390/v6052012 Article OPEN ACCESS viruses ISSN 1999-4915 www.mdpi.com/journal/viruses Dynamics of the Presence of Israeli Acute Paralysis Virus in Honey Bee Colonies
More informationEssential Oils and Organic Acids for the Control of Varroa destructor in Honey Bees (Apis mellifera)
Rusty Burlew July 12, 2009 Essential Oils and Organic Acids for the Control of Varroa destructor in Honey Bees (Apis mellifera) It wasn t until 1986 that parasitic Varroa mites were introduced into the
More informationBiosynthesis and Seasonal Variation of Ethyl Oleate, a Primer Pheromone of the Honey Bee (Apis mellifera L.)
Biosynthesis and Seasonal Variation of Ethyl leate, a Primer Pheromone of the Honey Bee (Apis mellifera L.) Carlos Castillo 1,3, Hao Chen 1, Carolyn Graves 1, Alban Maisonnasse 2, Yves Le Conte 2 and Erika
More informationHoney bee colony vitality
Honey bee colony vitality Are we wintering summer bees? Impact of environment on status of the colony J. van der Steen Oct12 Content Vitality / superorganism Status description of the honey bee colony
More informationSelected High and Low Pollen Hoarding Colonies of Apis mellifera for Apple Foraging Preference
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (2017) pp. 2233-2244 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.610.264
More informationT H E H O N E Y B E E T I M E
Vol 1 Iss 7 T H E H O N E Y B E E T I M E S OFFICERS President Dennis Eck denscritters@yahoo.com V i c e P r e s i d e n t Larry Theurer larry1724@aol.com Treasurer Allyson May abjmay99@yahoo.com Secretary
More informationDATA REQUIREMENTS AND RISK ASSESSMENT FOR BEES PLANT PROTECTION. National approach for Belgium
PLANT PROTECTION DATA REQUIREMENTS AND RISK ASSESSMENT FOR BEES National approach for Belgium Federal Public Service Health, Food Chain Safety and Environment Service Plant protection products and Fertilizers
More informationAMERICAN FOULBROOD CONTROL
AMERICAN FOULBROOD CONTROL Dr Mark Goodwin Apicultural Research Unit HortResearch New Zealand This is the first article of a series that has been written for the Management Agency on the American Foulbrood
More informationHelp save the Bumblebee... get more buzz from your garden
Help save the Bumblebee... get more buzz from your garden Over the past seventy years, many kinds of bumblebees have become increasingly scarce; and two species have become extinct. Those species which
More informationKANAWHA VALLEY BEEKEEPERS ASSOCIATION
KANAWHA VALLEY BEEKEEPERS ASSOCIATION MEDICATIONS FOR HEALTHY HIVES API Life VAR API Life VAR is used to combat the Varroa mite. The varroa mite shows no resistance to it and it is not harmful to the bees
More informationTitle: Spread of infectious Chronic bee paralysis virus (CBPV) by honey bee (Apis mellifera L.) faeces ACCEPTED
AEM Accepts, published online ahead of print on 12 October 2007 Appl. Environ. Microbiol. doi:10.1128/aem.01053-07 Copyright 2007, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationLecture 10 Immune System
Lecture 10 Immune System Lecture 10 1. Introduction 2. Nonspecific External Defenses 3. Innate Immune Response 4. Acquired Immune Response 5. Antibiotics and Vaccines 1 The not-so-common cold A cold is
More information