Immunodiagnostic Systems
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1 Immunodiagnostic Systems Manual Immunoassay Product Menu
2 Vitamin D 25-Hydroxy Vitamin D EIA Enzymeimmunoassay for the quantitative IVD AC-57F1 96 Wells determination of 25-hydroxyvitamin D (25-OHD) and other hydroxylated metabolites 5 nmol/l (2 ng/ml) 4 hours total assay time; 45 minutes hands-on time 25-Hydroxy Vitamin D RIA Radioimmunoassay for the quantitative IVD AA-35F1 100 Tubes determination of 25-hydroxyvitamin D (25-OHD) and other hydroxylated metabolites 50 μl 3 nmol/l (1.2 ng/ml) 3 hours total assay time; 60 minutes hands-on time 1,25-Dihydroxy Vitamin D EIA Complete assay system for the purification IVD AC-62F1 96 Wells of 1,25D by immunoextraction followed by quantitation with enzymeimmunoassay 500 µl 6 pmol/l (2.5 pg/ml) Proprietary immunoextraction system 1,25-Dihydroxy Vitamin D RIA Complete assay system for the purification IVD AA-54F1 40 Columns of 1,25D by immunoextraction followed by quantitation with immunoassay AA-54F2 56 Columns 500 µl 2.1 pg/ml (5 pmol/l) Detects both 1,25-(OH)2 D 2 and D 3 Proprietary immunoextraction system No organic waste
3 Bone Turnover Markers Alpha CrossLaps (CTX-I) EIA Quantification of degradation of non- isomerised IVD AC-04F1 96 Wells C-terminal telopeptides of Type l collagen (CTX-I) Urine 0.80 ng/ml 1.5 hours total assay time; 30 minutes hands-on time BoneTRAP (TRAcP 5b) ELISA Quantitative determination of the active isoform IVD SB-TR201A 96 Wells of the tartrate-resistant acid phosphatase form (TRAcP 5b) Human serum, EDTA plasma 100 μl <0.5 U/L TRAcP 5b is derived exclusively from bone-resorbing osteoclasts No accumulation of TRAcP 5b activity into the circulation in renal or hepatic failure N-MID Osteocalcin ELISA Quantitative determination of Osteocalcin IVD AC-11F1 96 Wells 20 μl 0.5 ng/ml Recognises both intact and large N-MID Osteocalcin fragments with equal affinity Ostase + BAP EIA Quantitative measurement of bone specific IVD AC-20F1 96 Wells alkaline phosphatase as an indicator of osteoblastic activity Human serum 50 μl 0.7 μg/l 1 hour 15 minutes total assay time; <30 minutes hands-on time
4 Bone Turnover Markers Serum CrossLaps (CTX-I) ELISA Quantification of degradation products of IVD AC-02F1 96 Wells C-terminal telopeptides of Type l collagen (CTX-I) 50 μl ng/ml 3 hours total assay time; 30 minutes hands-on time Urine BETA CrossLaps (CTX-I) Quantification of degradation products of IVD AC-05F1 96 Wells ELISA C-terminal telopeptides of Type-l collagen (CTX-I) Human urine 10 μl 0.80 μg/l 3 hours total assay time; 30 minutes hands-on time Urine CrossLaps (CTX-I) EIA Quantification of degradation products of IVD AC-03F1 96 Wells C-terminal telopeptides of Type-l collagen (CTX-I) Human urine 15 ul 50 μg/l 3 hours total assay time; 45 minutes hands-on time
5 Cartilage Markers Serum Pre-Clinical CartiLaps Assay for the quantitative determination of degradation RUO AC-08F1 96 Wells (CTX-II) ELISA products of C-terminal telopeptides of type II collagen (CTX-II) 100 μl 1.2 pg/ml Multi-species assay Reflects cartilage degradation activity Urine CartiLaps (CTX-II) EIA Assay for the quantification of degradation IVD AC-10F1 96 Wells products of C-terminal telopeptides of type II collagen (CTX-II) Urine 40 μl 0.20 μg/l May be used as an aid for assessment of structural damage of articular cartilage in patients with RA and OA Urine Pre-Clinical CartiLaps Assay for the quantitative determination of RUO AC-09F1 96 Wells (CTX-II) EIA degradation products of C-terminal telopeptides of type II collagen (CTX-II) Non-human urine or cartilage explant culture supernatant 10 μl 0.75 μg/l Reflects cartilage degradation activity Multi-species application Growth Factors Insulin-like Growth Factor-I (IGF-I) Assay for the quantitative determination of IGF-I IVD AC-27F1 96 Wells ELISA or plasma 3.1 μg/l Method incorporates a sample pre-treatment to avoid binding protein interference Results within 4 hours Insulin-like Growth Factor-I Immunoradiometric assay for the determination IVD CL-BC Tubes (IGF-I) IRMA of IGF-I 1.25 ng/ml 2 monocolonal antibodies with high affinity and specificity are used which recognise two different epitopes of IGF-I Insulin-like Growth Factor Binding Immunoradiometric assay for the determination IVD CL-BC Tubes Protein-3 (IGFBP-3) IRMA of IGFBP-3 10 μl 50 ng/ml A one step solid phase immunoradiometric assay A single IGFBP-3 determination is an excellent screening parameter for Growth Hormone Deficiency
6 Animal Research CrossLaps for Culture (CTX-I) Quantitative determination of fragments of RUO AC-07F1 96 Wells ELISA type I collagen released into bone cell culture supernatants (CTX-I) Cell culture supernatant 50 μl 0.44 nm 3 hours total assay time; 45 minutes hands-on time Can detect osteoclastic activity on bone slices using culture conditions in multi-well screening formats Mouse Insulin-like Growth Factor-I Assay for the quantitative determination of IGF-I RUO AC-42F1 96 Wells High (IGF-I HS) ELISA Mouse serum or plasma 10 μl 2.8 ng/ml Can be used with knock out (LID or ALSKO) mice, and some tissue and cell culture media Multiple sample types Serum, EDTA or heparinized plasma give similar values and may be used interchangeably MouseTRAP (TRAcP 5b) ELISA Quantitative determination of osteoclast- RUO SB-TR Wells derived tartrate-resistant acid phosphatase form (TRAcP 5b) in mice 0.1 U/L TRAcP 5b is derived exclusively from bone-resorbing osteoclasts No accumulation of TRAcP 5b activity into the circulation in renal or hepatic failure RatLaps (CTX-I) EIA Quantitative determination of fragments of type I RUO AC-06F1 96 Wells collagen in rats/mice and from bone released into cell culture supernatants by osteoclasts (CTX-I) Rat/mouse serum, urine, cell culture supernatant 20 μl 2 ng/ml Possibility of measuring both urine and serum samples Low sample volume 60 minutes hands-on time Rat-MID Osteocalcin EIA Quantitative determination of Osteocalcin from rats RUO AC-12F1 96 Wells, plasma (EDTA, heparin) 20 μl 50 ng/ml Low sample volume Rat/Mouse Insulin-like Growth Assay for the quantitative determination of IGF-I RUO AC-18F1 96 Wells Factor-I (IGF-I) ELISA* or plasma 25 µl (alternative 10 µl) 63 ng/ml Ideal specificity characteristics no cross-reactivity with IGF-II or human IGF-I Co-specific for both rat and mouse IGF-I
7 Animal Research Rat/Mouse PINP EIA Quantitative determination of N-terminal RUO AC-33F1 96 Wells propeptide of type I procollagen (PINP) in rats/mice, plasma (EDTA, heparin) 5 μl 0.7 ng/ml Use in conjunction with IDS RatTRAP Low sample volume RatTRAP (TRAcP 5b) ELISA Quantitative determination of osteoclast-derived RUO SB-TR Wells tartrate-resistant acid phosphatase form (TRAcP 5b) in rats 0.1 U/L TRAcP 5b is derived exclusively from bone-resorbing osteoclasts No accumulation of TRAcP 5b activity into the circulation in renal or hepatic failure Others Bone Slices For the in vitro assessment of osteoclastic RUO DT-1BON 50 Pieces bone resorption Cortical bone slices from the femur of bovine bones Corticosterone EIA Assay for the quantitative determination of RUO AC-14F1 96 Wells corticosterone Rat and mouse serum or plasma 30 μl 0.55 ng/ml Non-extraction - a simple dilution step replaces tedious solvent extraction/evaporation Corticosterone HS Assay for the quantitative determination of RUO AC-15F1 96 Wells (High ) EIA corticosterone or plasma 100 μl for duplicate determination 0.17 ng/ml Multiple species application - ideal tool for researchers investigating metabolic status and stress levels in various animal species Dentine Discs For use as bone resorption substrates RUO AE Discs 5mm diameter wafers of devitalised dentine AE Discs RUO Research Use Only. IVD In Vitro Diagnostic Use. + Ostase is a registered trademark of Hybritech Incorporated, a subsidiary of Beckman Coulter. Inc.
8 For more information on the IDS products, contact your local IDS office or contact IDS UK for the contact information of your local distributor. UK Germany 10 Didcot Way Mainzer Landstrasse 49 Boldon Business Park Frankfurt am Main Boldon Germany Tyne & Wear Tel: +49 (0) NE35 9PD Fax: +49 (0) Tel: +44 (0) Fax: +44 (0) Belgium Scandinavia 101, rue Ernest Solvay International House B 4000 Liége Center Boulevard 5 Belgium 2300 København S Tel: +32 (0) Denmark Fax: +32 (0) Tel: info.be@idsplc.com info.nordic@idsplc.com France USA 153 avenue d Italie 8425 N. 90th Street, Suite Paris Scottsdale, AZ France USA Tel: +33 (0) Tel: Fax: +33 (0) Fax: info.fr@idsplc.com info.us@idsplc.com Order: P.ListIntl, Issue 4, All rights reserved 2012 Immunodiagnostic Systems
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