ab FirePlex mirna Panel Kidney Toxicity
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1 Version 1 Last updated 30 May 2017 ab FirePlex mirna Panel Kidney Toxicity This product is for research use only and is not intended for diagnostic use. Copyright 2017 Abcam. All rights reserved. FirePlex
2 Table of Contents 1. Overview 2 2. FirePlex mirna Panel Kidney Toxicity 3 3. Notes 7 ab mirna Panel Kidney Toxicity 1
3 1. Overview The FirePlex Kidney toxicity panel has been designed to profile expression of 65 mirnas () that have been demonstrated in multiple peer-reviewed studies to be differentially regulated as a part of kidney toxicity or acute kidney injury. Our multiplex mirna assays use our FirePlex particle technology to profile up to 65 mirnas per well. With the same sensitivity as PCR, they can be used either directly with 10 µl of biofluids such as serum or plasma with no need for RNA purification; or with 100 pg of purified RNA. For a full list of validated sample types, and to learn more about the performance and requirements of these assays, visit These mirnas have been chosen based on a detailed study of data in peer-reviewed journals and exhibit differential regulation in plasma, serum, urine, isolated exosomes or total RNA from kidney or kidney-derived cell lines. Included in the panel are general markers for multiple kidneyrelated diseases, markers specific to specific kidney states or treatment responses, markers that may indicate hemolysis contamination in isolated plasma/serum/exosomes and internal controls are included to validate assay performance. It is important to use the normalization procedure in the FirePlex Analysis Workbench Software to identify the mirnas that can optimally be used for normalization with your samples. Three mirnas that are likely to be stably expressed in biofluid samples and may be suitable have been included in this panel. ab mirna Panel Kidney Toxicity 2
4 2. FirePlex mirna Panel Kidney Toxicity To use this product researchers must also purchase the appropriate Core Reagent Kit, either ab for use with purified RNA or ab for use with biofluids (see these kits for the full assay protocol). FirePlex is a registered trade mark in the United States and is an unregistered trade mark elsewhere. This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us. ** Hsa = Homo sapiens (human), Mmu = Mus musculus (mouse), Rno = Rattus norvegicus (rat), Ath = Arabidopsis thaliana (arabidposis), Oan = Ornithorhynchus anatinus (platypus), Cel = Caenorhabditis elegans (worm). # mirna 21 name mirna Sequence (mirbase V21) 1 hsa-let-7c-5p UGAGGUAGUAGGGUAUGG 2 hsa-let-7e-5p UGAGGUAGGAGGGUAUAG 3 hsa-let-7g-5p UGAGGUAGUAGUGUACAG 4 hsa-mir-100-5p AACCCGUAGAUCCGAACG UG 5 hsa-mir-10a-5p UACCCUGUAGAUCCGAAU GUG 6 hsa-mir-122-5p UGGAGUGUGACAAUGGUG UG 7 hsa-mir-125b-5p UCCCUGAGACCCUAACGU GA 8 hsa-mir-126-3p UCGUACCGUGAGUAAUAAUG CG 9 hsa-mir-130a-3p CAGUGCAAUGAAAAGGGC AU 10 hsa-mir-130b-3p CAGUGCAAUGAUGAAAGGG CAU 11 hsa-mir-132-3p UAACAGUCUACAGCCAUGGU CG Sequence homology ** MIMAT ID MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT hsa-mir-134-5p UGUGACUGGGACCAGAG MIMAT ab mirna Panel Kidney Toxicity 3
5 GGG 13 hsa-mir-138-5p 14 hsa-mir-140-3p 15 hsa-mir-142-3p 16 hsa-mir-143-3p 17 hsa-mir-145-5p 18 hsa-mir-146a-5p 19 hsa-mir-146b-5p 20 mmu-mir-155-5p 21 hsa-mir-15a-5p 22 hsa-mir-15b-5p 23 hsa-mir-16-5p 24 hsa-mir-17-5p 25 hsa-mir-181a-5p 26 mmu-mir-182-5p 27 rno-mir-183-5p 28 hsa-mir-186-5p 29 hsa-mir-18a-5p 30 hsa-mir-191-5p 31 hsa-mir-192-5p 32 hsa-mir-194-5p 33 hsa-mir-19a-3p 34 hsa-mir-200a-3p 35 hsa-mir-200c-3p 36 hsa-mir-203a-3p 37 hsa-mir-206 AGCUGGUGGUGAAUCAG GCCG UACCACAGGGUAGAACCAC GG UGUAGUGUCCUACUAUG GA UGAGAUGAAGCACUGUAGCU C GUCCAGCCCAGGAAUC CCU UGAGAACUGAACCAUGGG UGAGAACUGAACCAUAGG CU AAUGCUAAGUGAUAGG GGU UAGCAGCACAUAAUGGUG UG UAGCAGCACAUCAUGGUA CA UAGCAGCACGUAAAUAGG CG CAAAGUGCACAGUGCAG GUAG AACACAACGCUGUCGGUG AGU UGGCAAUGGUAGAACUCA CACCG UAUGGCACUGGUAGAACA CU CAAAGAACUCCGGG CU UAAGGUGCAUCUAGUGCAGA UAG CAACGGAAUCCCAAAAGCA GCUG CUGACCUAUGAAGACAGC C UGUAACAGCAACUCCAUGUG GA UGUGCAAAUCUAUGCAAAAC UGA UAACACUGUCUGGUAACGAU GU UAAUACUGCCGGGUAAUGAU GGA GUGAAAUGUAGGACCACU AG UGGAAUGUAAGGAAGUGUGU GG MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT ab mirna Panel Kidney Toxicity 4
6 38 hsa-mir-20b-5p 39 hsa-mir-210-3p 40 mmu-mir-212-3p 41 hsa-mir-214-3p 42 hsa-mir-21-5p 43 hsa-mir-218-5p 44 hsa-mir-223-3p 45 hsa-mir-23b-3p 46 hsa-mir-26b-5p 47 hsa-mir-27a-3p 48 hsa-mir-29a-3p 49 hsa-mir-30a-5p 50 hsa-mir-30c-5p 51 hsa-mir-30e-3p 52 hsa-mir-320a 53 hsa-mir-342-3p 54 hsa-mir-34a-5p 55 hsa-mir-34c-5p 56 mmu-mir-378a- 3p 57 hsa-mir-409-3p 58 hsa-mir-455-5p 59 hsa-mir hsa-mir-486-5p 61 mmu-mir-489-3p 62 hsa-mir-494-3p 63 hsa-mir-532-3p CAAAGUGCUCAUAGUGCAG GUAG CUGUGCGUGUGACAGCGGC UGA UAACAGUCUCCAGUCACGG CCA ACAGCAGGCACAGACAGGC AGU UAGCAUCAGACUGAUG GA GUGCGAUCUAACCAUG U UGUCAGUGUCAAAUACCC CA AUCACAGCCAGGGAAC C CAAGUAACAGGAUAGG U CACAGUGGCUAAGCCG C UAGCACCAUCUGAAAUCGGU UA UGUAAACAUCCUCGACUGGA AG UGUAAACAUCCUACACUCUC AGC CUCAGUCGGAUGUACA GC AAAAGCUGGGGAGAGGG CGA UCUCACACAGAAAUCGCACC CGU UGGCAGUGUCAGCUGG GU AGGCAGUGUAGAGCUGAU UGC ACUGGACGGAGUCAGAA GG GAAUGGCUCGGUGAACC CCU UAUGUGCCUGGACUACAU CG UCAGGCUCAGUCCCCUCCC GAU UCCUGUACUGAGCUGCCCC GAG AAUGACACCACAUAUAUGGC AGC UGAAACAUACACGGGAAAC CUC CCUCCCACACCCAAGGC GCA MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT MIMAT ab mirna Panel Kidney Toxicity 5
7 64 hsa-mir-93-5p 65 hsa-mir-98-5p 66 ath-mir167d 67 cel-mir-70-3p 68 oan-mir p CAAAGUGCUGCGUGCAG GUAG UGAGGUAGUAAGGUAG UGAAGCUGCCAGCAUGAUCU GG UAAUACGUCGGGUGUC CAU CCCCACUCUGAGCACACA GC Ath Cel Oan 69 x-control x-control N/A 70 blank blank N/A MIMAT MIMAT MIMAT MIMAT MIMAT ab mirna Panel Kidney Toxicity 6
8 3. Notes ab mirna Panel Kidney Toxicity 7
9 Technical Support Copyright 2017 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. Austria wissenschaftlicherdienst@abcam.com France supportscientifique@abcam.com Germany wissenschaftlicherdienst@abcam.com Spain soportecientifico@abcam.com Switzerland technical@abcam.com Deutsch: Français: UK, EU and ROW technical@abcam.com +44(0) Canada ca.technical@abcam.com US and Latin America us.technical@abcam.com Asia Pacific hk.technical@abcam.com (852) China cn.technical@abcam.com Japan technical@abcam.co.jp +81-(0) Singapore sg.technical@abcam.com Australia au.technical@abcam.com +61-(0) New Zealand nz.technical@abcam.com +64-(0) Copyright 2017 Abcam. All rights reserved. FirePlex
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