Virbac innovation in Dermatology

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1 Virbac innovation in Dermatology

2 The Virbac Innovation in Dermatology A SYNERGISTIC ASSOCIATION / SPECIFIC ENCAPSULATION TECHNOLOGY SPHERULITES / USE OF "A MULTI-ACTIVE INGREDIENT" CHITOSANIDE (CHITOSAN DERIVATIVE)

3 What are Shperulites? MULTILAMELLAR MICROVESICLES MULTICONCENTRIC STRUCTURE SIMILAR TO AN ONION EACH MEMBRANE = BI-LAYER OF SURFACTANTS MICROSCOPIC AND CONTROLLED SIZE 0.2 to 20 µm NEW AND EXCLUSIVE ACTIVE CARRIER PATENTED TECHNOLOGY (CNRS / VIRBAC) Spherulites structure

4 What are Shperulites? Detail of tensioactive layers and principle of simultaneous encapsulation of hydrophilic and lipophilic active ingredients. LIPOPHILIC COMPARTMENT HYDROPHILIC COMPARTMENT hydrophilic active ingredient lipophilic active ingredient surfactant

5 What are Shperulites? Spherulites in Scanning Electron Microscopy: Cliché Tadek GULIK

6 Comparison Spherulites vs. Liposomes CHARACTERISTICS SPHERULITES LIPOSOMES STRUCTURE MULTILAMELLAR VESICLES ( LAYERS) MONO/OLIGO LAMELLAR VESICLES (1-10 LA YER S) C O M P O S ITIO N O F MEMBRANES WIDE RANGE OF SURFACTANTS (CATIONICS, NON IONICS AMPHOTERICS) PHOSPHOLIPIDS (LEC ITH IN S) ENCAPSULATED IN G R E D IE N TS ACTION LEVEL PHYSICAL STABILITY WIDE RANGE : HYDROPHILIC LIPOPHILIC SMALL MACRO => SIMULTANEOUS ENCAPSULATION ADJUSTABLE: SURFACE SUPERFICIAL DEEP M O L E C U L E S STABLE (FULL VESICLES) M A IN LY : HYDROPHILIC M O LE C U LE S DEEP ACTION UNSTABLE (EMPTY SPACES)

7 Technical Advantages CHRONO-RELEASE / PERSISTENCE OF ACTIVE INGREDIENTS encapsulated ingredients are released progressively during membrane disruption SPECIFIC ACTION LEVEL adjustable penetration depending on the membrane surfactant composition CATIONIC SPHERULITES POSITIVE EXTERNAL CHARGES SURFACE ACTION NON IONIC SPHERULITES NO EXTERNAL CHARGES DEEP ACTION

8 Practical Advantages PROGRESSIVE AND LONG TERM EFFECTS IMPROVED TOLERANCE AND EFFICIENCY

9 What is Chitosanide? CHARACTERISTICS NATURAL BIOPOLYMER DERIVED FROM CHITOSAN (CRUSTACEAN SHELLS) NON TOXIC, NON ALLERGENIC, BIODEGRADABLE MATERIAL

10 Chitosanide A multi-active Ingredient COSMETIC PROPERTIES = FILMING ACTION AND PERSISTENCE High affinity for hair, skin and nails Protective elastic film on the surface = SKIN MOISTURIZING AGENT (Sachetto et al, 1986) Water retention capacity similar to hyaluronic acid = SOOTHING AND SKIN REGENERATING FACTOR (Okamoto et al, 1995)

11 Chitisanide Skin Adhesion mechanism unit glucosamine active ingredient cationic groupment biological surfaces with negative charge keratins

12 Interest of Chitosanide MEDICAL PROPERTIES AND APPLICATIONS = WOUND MANAGEMENT Artificial skin for burn treatment (BESCHITIN R ) Contact lenses for corneal lesions (BARNES HINDS Inc.) = SURGERY Suture Thread = CONTROLLED RELEASE OF DRUGS (FUJI SPINNING Co. Ltd.)

13 Virbac Innovation: Synergistic Association Spherulites + Chitosanide WHY DO WE INCLUDE CHITOSANIDE?» BIOADHESION Cationic molecule CHITOSANIDE IMPROVES» LONG-TERM EFFECT Filming action» STABILITY Structure cohesion VIRBAC PATENT

14 Bio-adhesion Mechanism DISPERSION AND ADHESION OF SPHERULITES FUSION OF SPHERULITES / ACTIVE RELEASE FORMATION OF THE CHITOSANIDE FILM 1 2 3

15 Visualization Tests on Hair (Optical Microscopy) Non treated hair (x400) Treated hair (x400)

16 Visualization Tests on Hair (Optical Microscopy) (x100) Treated hair (x200)

17 Visualization Tests on Hair (Optical Microscopy) Hair after drying (x400)

18 Visualization Tests on Hair (Electronic Microscopy) a b c d e f

19 Persistence of Spherulites (Outdoor Test) a - Before treatment b - After treatment Day 0 c - Day 4 d - Day 10

20 Improved active ingredients diffusion in skin by vehicle Distribution of 14 C-labelled non-ionic Spherulites in canine skin biopsies epidermis Spherulites derma Autoradiography on frozen sections Non-ionic Spherulites diffuse through epidermal cell layers, hair follicle & sebaceous glands Barthe N., Jasmin P., Brouillaud B., Guinez C., Coulon P. & Gatto H. Assessment of the Bio-distribution of non-ionic spherulites in dog skin. Proceedings 16th ESVD-ECVD Congress, Helsinki, Finland, 1999

21 Specificities of Virbac Dermatology Products FREE ACTIVE INGREDIENTS immediate action MICRO-ENCAPSULATED DRUGS long-term effect Deep Action FREE AND MICRO-ENCAPSULATED CHITOSANIDE increased bio-adhesion and persistence protection and hydration